KR20220071014A - Analysis well strip - Google Patents

Abstract

The present invention relates to a reaction well strip which allows a biochemical reaction or a biological reaction to be smoothly performed. The reaction well strip comprises: a strip body unit formed long in a longitudinal direction; a plurality of reaction body units in which plurality form at least one row and are disposed at regular intervals on the strip body unit and an inlet formed thereon so that at least one object to be analyzed can be injected therein; and a reaction unit formed in the inside of the reaction body unit and fixing a reactant capable of reacting with the object to be analyzed.

Description

Reaction well strip {Analysis well strip}

The present invention relates to a reaction well strip, and more particularly, to a reaction well strip that enables a smooth biochemical reaction or biological reaction.

In recent years, various chemiluminescence (CL) methods have been developed, for example, enzyme immunoassay (ELIA) using an antigen-antibody reaction, and chemiluminescence compounds as tracers for immunoassays. Chemiluminescence immunoassay (CLIA) used for labeling and chemiluminescent enzyme immunoassay (CLEIA), which detects enzyme activity with high sensitivity using a chemiluminescent compound in a detection system, etc. There is this.

In this immunoassay, the presence and concentration of the analyte is confirmed through the selective reaction (biospecific binding) of antigens and antibodies. In this process, materials that do not react selectively are removed through washing before signal measurement.

Through this cleaning, it is possible to increase the sensitivity of the signal by lowering the background signal.

Capillary-driven flow using capillary force uses a phenomenon in which a fluid flows automatically by surface tension without an external pump. Many simple disposable immunoassay products such as pregnancy diagnostic reagents have been developed using this capillary flow. Most of these products use porous materials to induce capillary flow and cannot perform more than two washes.

In addition, US Patent No. 6,271,040 generates capillary flow in a microchannel without using a porous material. However, although capillary force is used in the above products and patents, since the microfluid is cleaned with only a sample solution, there is a problem in that it takes a lot of time and money for analysis.

Therefore, there was an urgent need to develop a technology for a reaction well strip capable of analyzing a large amount or multiple types of analytes at the same time.

The idea of the present invention is to solve these problems, and it is possible to analyze a large amount and various kinds of analytes at the same time, to arrange M rows and N columns on a tray to enable various analyzes, and structurally the reaction of the reaction part An object of the present invention is to provide a reaction well strip that can promote, easily attach and detach from a tray, and apply a structure excellent in strength during attachment and detachment to safely and easily perform analysis.

Reaction well strip according to the spirit of the present invention for solving the above problems, a strip body portion formed long in the longitudinal direction; a plurality of reaction body parts arranged at regular intervals in the strip body part to form at least one row, and having an inlet formed thereon so that at least one analysis target can be input; and a reaction unit that is formed inside the reaction body and may be fixed with a reactant capable of reacting with the analyte.

In addition, according to the present invention, at least one locking protrusion formed in the shape of an arrowhead to be engaged with the tray on the side or both sides of the strip body portion; may further include.

In addition, according to the present invention, a reinforcing rib extending from the strip body portion to reinforce strength and formed between the reaction body portion and other adjacent reaction body portions; may further include.

In addition, according to the present invention, the reinforcing rib, at least one intermediate rib formed in the middle portion of the strip body; and at least one end rib formed at both ends of the strip body, wherein a first length of the intermediate rib may be formed to be longer than a second length of the end rib.

In addition, according to the present invention, the diameter of the inlet is larger than the area of the bottom, and the reaction unit may include a funnel-shaped inclined surface as a whole so that the diameter can be continuously decreased from the inlet to the bottom.

In addition, according to the present invention, the reaction unit may have a radiating protrusion formed in a radial shape around the bottom portion on the inclined surface so as to increase the surface area of the reaction surface.

In addition, according to the present invention, the reflective protrusion may include: a first inclined surface portion formed at a first angle with respect to a horizontal plane in contact with the bottom portion; a second inclined surface part connected to the first inclined surface part and formed at a second angle with respect to the horizontal plane; and a third inclined surface part connected to the second inclined surface part and formed at a third angle with respect to the horizontal plane in contact with the inlet port, wherein the first part to facilitate accommodation of the analysis object through the bottom part The angle may be greater than the second angle, and the second angle may be greater than the third angle.

Also, according to the present invention, the analyte may be a biological sample or antigen, and the reactant may be a binding-inducing substance or antibody capable of inducing bio-specific binding.

In addition, according to the present invention, the reaction body portion is inserted into the cartridge, the inlet is formed at the upper side to facilitate dispensing the analyte from the dispenser through the inlet, the upper body is formed in a cylindrical shape as a whole; and a lower body formed in a funnel shape as a whole under the upper body to facilitate accommodation of the analyte through the bottom.

According to some embodiments of the present invention made as described above, it is possible to analyze a large amount and various kinds of analytes at the same time, and it is possible to enable various analyzes by arranging M rows and N columns on a tray, and structurally the reaction of the reaction part It has the effect of enabling safe and easy analysis by applying a structure that is excellent in strength during attachment and detachment at the same time as it is easy to attach and detach from the tray. Of course, the scope of the present invention is not limited by these effects.

1 is a perspective view illustrating a reaction well strip in use according to some embodiments of the present invention.
FIG. 2 is an external perspective view illustrating the reaction well strip of FIG. 1 ;
FIG. 3 is a partially cut-away perspective view illustrating the reaction well strip of FIG. 2 .
4 is a cross-sectional view illustrating the reaction well strip of FIG. 3 .
5 is an enlarged cross-sectional view of a reaction part of the reaction well strip of FIG. 4 .
6 is a side view illustrating the reaction well strip of FIG. 4 ;
7 is a perspective view illustrating a locking protrusion of the reaction well strip of FIG. 4 .

Hereinafter, various embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Examples of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art, and the following examples may be modified in various other forms, and the scope of the present invention is as follows It is not limited to an Example. Rather, these embodiments are provided so as to more fully and complete the present disclosure, and to fully convey the spirit of the present invention to those skilled in the art. In addition, in the drawings, the thickness or size of each layer is exaggerated for convenience and clarity of description.

The terminology used herein is used to describe specific embodiments, not to limit the present invention. As used herein, the singular form may include the plural form unless the context clearly dictates otherwise. Also, as used herein, “comprise” and/or “comprising” refers to specifying the presence of the recited shapes, numbers, steps, actions, members, elements, and/or groups thereof. and does not exclude the presence or addition of one or more other shapes, numbers, movements, members, elements and/or groups.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described with reference to the drawings schematically illustrating ideal embodiments of the present invention. In the drawings, variations of the illustrated shape can be envisaged, for example depending on manufacturing technology and/or tolerances. Accordingly, embodiments of the spirit of the present invention should not be construed as limited to the specific shape of the region shown in the present specification, but should include, for example, changes in shape caused by manufacturing.

Hereinafter, a reaction well strip according to various embodiments of the present invention will be described in detail with reference to the drawings.

1 is a perspective view showing a reaction well strip 100 in use according to some embodiments of the present invention, FIG. 2 is an external perspective view showing the reaction well strip 100 of FIG. 1 , and FIG. 3 is the reaction of FIG. 2 . It is a partially cut-away perspective view showing the well strip 100 , and FIG. 4 is a cross-sectional view showing the reaction well strip of FIG. 3 .

First, as shown in FIGS. 1 to 4 , the reaction well strip 100 according to some embodiments of the present invention includes a strip body part 110 , a reaction body part 10 , and a reaction part 20 . ) may be included.

For example, as shown in FIGS. 1 to 4 , the strip body part 110 may be a thin rod-shaped structure elongated in the longitudinal direction as being engaged with the tray TR.

In addition, for example, as shown in Figures 1 to 4, the reaction body portion 10, a plurality (eight in the drawing) form at least one row, arranged in the strip body portion 110 at regular intervals It may be a kind of tubular structure in which the input port 10a is formed at the upper side so that at least one or more analysis objects 1 can be put in, and the bottom part 10b is formed at the lower side.

As shown in FIGS. 1 to 4, for example, the reaction body portion 10 may be formed integrally with the strip body portion 110 and the reaction portion 20 to be described later, It may be made of a synthetic resin material such as a plastic or polymer material that does not affect the reaction or has a small effect.

7 is a perspective view illustrating a locking protrusion of the reaction well strip of FIG. 4 .

In addition, for example, as shown in FIGS. 1 to 4 and 7 , the reaction well strip 100 according to some embodiments of the present invention is provided with trays TR on the side or both sides of the strip body 110 . ) and at least one locking protrusion 111 formed in an arrowhead shape to engage and engage and extend from the strip body part 110 to reinforce the strength, and another reaction body part adjacent to the reaction body part 10 It may further include a reinforcing rib 112 formed between (10).

More specifically, for example, as shown in FIGS. 1 to 4 , the reinforcing rib 112 includes at least one intermediate rib 112a formed in the middle portion of the strip body 110 and the strip. It may include at least one end rib (112b) formed on both ends of the body portion (110).

Here, when the strip body part 110 is lifted and forcibly separated from the tray TR of FIG. 1 , the middle part of the strip body part 110 on which an external force is concentrated can be structurally reinforced. The first length L1 of the rib 112a may be longer than the second length L2 of the end rib 112b.

Accordingly, as shown in FIGS. 1 to 4 , a plurality of reaction well strips 100 according to some embodiments of the present invention are prepared and can be easily attached to and detached from the tray TR. The analyte 1 can be analyzed at the same time, and various analyzes can be made by arranging M rows and N columns on the tray TR, structurally it is possible to promote the reaction of the reaction part, and it is easy to attach and detach from the tray At the same time, the analysis can be performed safely and easily by applying a structure that is superior in strength during attachment and detachment.

FIG. 5 is an enlarged cross-sectional view of the reaction part 20 of the reaction well strip 100 of FIG. 4 , and FIG. 6 is a side view illustrating the reaction well strip 100 of FIG. 4 .

1 to 6, more specifically, for example, the reaction body portion 10, the reaction body portion 10, is inserted into the cartridge through the inlet (10a) The inlet 10a is formed at the upper side to facilitate dispensing of the analysis object 1 from the cycle, and the analysis object 1 is received through the upper body 11 and the bottom which are formed in a cylindrical shape as a whole. It may include a lower body 12 formed in the form of a funnel as a whole under the upper body 11 to facilitate. Of course, the upper body 11 and the lower body 12 may be integrally formed.

Here, as shown in FIGS. 1 to 6 , the dispensing of the analyte 1 is made quickly, and after a sufficient reaction time, the inlet 10a so that the reception of the analyte 1 can be smoothly made. ) may have a diameter D1 greater than an area of the bottom 10b.

Therefore, the analyte 1 may be put into the inside of the body 10 through the inlet 10a, and after a certain reaction time, it is accommodated in the bottom part 10b so that the reaction can be smoothly performed. .

In addition, for example, as shown in FIGS. 1 to 6 , the reaction unit 20 is a part formed inside the reaction body unit 10 , and a reaction capable of reacting with the analyte 1 . It may be a part to which the sieve 3 can be fixed.

More specifically, for example, the reaction unit 20 may include an inclined surface 21 having a funnel shape as a whole so that the diameter can be continuously decreased from the inlet 10a to the bottom 10b.

As a result of repeated simulations and experiments, the angle of the inclined surface 21 may be excellent in terms of drainage and wettability to have a range of about 20 to 80 degrees.

Here, in the reaction unit 20, a plurality of radiating protrusions T may be formed on the inclined surface 21 in a radial shape with respect to the bottom portion 10b to increase the surface area of the reaction surface. .

As shown in FIG. 3 , nine of these radiating protrusions T are arranged at an isometric angle, but in addition, a minimum of three to 13 may be formed. Here, in the case of two or less, the effect of increasing the surface area is deteriorated, and in the case of 14 or more, wettability may be deteriorated.

More specifically, for example, as shown in FIGS. 1 to 6 , the reflective protrusion T is in contact with the bottom part 10b and is formed at a first angle K1 with respect to the horizontal plane. The inclined surface portion T1, the second inclined surface portion T2 and the second inclined surface portion T2 connected to the first inclined surface portion T1 and formed at a second angle K2 with respect to the horizontal plane, It may include a third inclined surface portion T3 connected to the inlet 10a and formed at a third angle K3 with respect to the horizontal plane in contact with the inlet port 10a.

Here, the first angle K1 is greater than the second angle K2 so that the unreacted analyte 1 can be easily discharged through the bottom 10b, and the second angle K2 is It may be larger than the third angle K3.

For example, as a result of repeated simulations and experiments, the first angle may be approximately 80 degrees to 90 degrees, the second angle may be approximately 60 degrees to 80 degrees, and the third angle may be approximately 40 degrees to 60 degrees. And it can be excellent in wettability.

Also, for example, the analyte 1 may be a biological sample or antigen, and the reactant 3 may be a binding-inducing substance or antibody capable of inducing bio-specific binding.

Accordingly, when describing a specific method of using the reaction well strip 100 according to some embodiments of the present invention, a binding-inducing material capable of adsorption or biospecific binding in the reaction well strip 100 is produced by IL -6 As a specific antibody selected and fixed on the surface, for example, 1% concentration of glutaraldehyde diluted with 0.1M MES (pH6.1), 30uL sample solution is used to immobilize the surface for 30 minutes. can

Then, it can be washed once for 10 minutes using 30 uL of a cleaning solution composed of 0.1 M MES (pH 6.1). IL-6 specific antibody 10ug/mL, 30uL can be added and a reaction between glutaraldehyde and IL-6 antibody can be induced for 30 minutes.

Then, 2% BSA, 0.05% TritonX100-0.1M Tris (pH7.6) sample solution was added and reacted twice for 10 minutes, and the functional group of glutaraldehyde not bound on the surface in the reaction well strip 100 (functional group) ) can be removed.

Then, 30 uL of IL-6 antigen is added for each concentration to be analyzed, and binding to the antibody is allowed to occur for 30 minutes. Add 30uL of a cleaning solution composed of 0.05% TritonX100-Tris (pH7.6) and wash once for 10 minutes.

Then, 2ug/mL, 30uL of an antibody-biotin polymer is added and biospecific binding is induced for 30 minutes. Again, 30uL of a cleaning solution composed of 0.05% TritonX100-Tris (pH7.6) can be added and washed once for 10 minutes.

Then, 30 uL of streptavidin-HRP polymer can be added and biospecific binding between the antibody-biotin polymer and the streptavidin-enzyme polymer can occur for 30 minutes.

Subsequently, 30 uL of a cleaning solution composed of 0.05% TritonX100-Tris (pH7.6) is added and washed three times at 10-minute intervals to wash off unbound streptavidin-enzyme.

Finally, 30 uL of a substrate reacting with the enzyme is added and the reaction is performed for 15 minutes, and the amount of IL-6 antigen can be quantified. In this case, an analysis method such as absorption, fluorescence, or chemiluminescence may be determined according to the type of substrate reacting with the enzyme.

Although the present invention has been described with reference to the embodiments shown in the drawings, which are merely exemplary, those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. Therefore, the true technical protection scope of the present invention should be determined by the technical spirit of the appended claims.

TR: tray
1: analyte
2: unreacted analyte
3: reactant
10: reaction body part
10a: inlet
10b: bottom
11: Upper body
12: lower body
20: reaction part
21: slope
T: radial protrusion
T1: first inclined surface portion
T2: second inclined surface portion
T3: third inclined surface portion
K1: first angle
K2: second angle
K3: 3rd angle
100: reaction well strip
110: strip body portion
111: locking protrusion
112: reinforcing rib
112a: middle rib
112b: end rib
L1: first length
L2: second length

Claims (9)

Strip body portion formed to be long in the longitudinal direction;
a plurality of reaction body portions arranged at regular intervals in the strip body portion to form at least one row, and having an inlet formed at the upper side so that at least one analysis object can be input; and
a reaction unit formed inside the reaction body and to which a reactant capable of reacting with the analyte may be fixed;
A reaction well strip comprising a. The method of claim 1,
at least one locking protrusion formed in the shape of an arrowhead so as to be engaged with the tray on the side or both sides of the strip body;
A reaction well strip further comprising a. The method of claim 1,
a reinforcing rib extending from the strip body portion to reinforce strength and formed between the reaction body portion and another adjacent reaction body portion;
A reaction well strip further comprising a. 4. The method of claim 3,
The reinforcing rib is
at least one intermediate rib formed in a middle portion of the strip body; and
at least one end rib formed at both ends of the strip body;
including,
and a first length of the intermediate rib is formed to be greater than a second length of the end rib. The method of claim 1,
The diameter of the inlet is larger than the area of the bottom,
The reaction unit,
A reaction well strip comprising a funnel-shaped inclined surface as a whole so as to continuously decrease in diameter from the inlet to the bottom. 6. The method of claim 5,
The reaction well strip, wherein the reaction well strip is formed with a radiating protrusion formed in a radial shape with respect to the bottom portion on the inclined surface so as to increase the surface area of the reaction surface. 7. The method of claim 6,
The reflective protrusion,
a first inclined surface portion formed at a first angle with respect to a horizontal plane in contact with the bottom portion;
a second inclined surface part connected to the first inclined surface part and formed at a second angle with respect to the horizontal plane; and
A third inclined surface part connected to the second inclined surface part and formed at a third angle with respect to the horizontal plane in contact with the inlet port;
wherein the first angle is greater than the second angle and the second angle is greater than the third angle to facilitate accommodation of the analyte through the bottom portion. The method of claim 1,
The reaction well strip, wherein the analyte is a biological sample or antigen, and the reactant is a binding-inducing substance or antibody capable of inducing bio-specific binding. The method of claim 1,
The reaction body part,
The upper body is inserted into the cartridge and the inlet is formed at the upper side to facilitate dispensing the analyte from the dispenser through the inlet, and is formed in a cylindrical shape as a whole; and
a lower body formed in a funnel shape as a whole under the upper body to facilitate accommodation of the analyte through the bottom;
A reaction well strip comprising a.

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