KR102569852B1 - Analysis well strip - Google Patents

Abstract

[0001] The present invention relates to a reaction well strip enabling smooth biochemical or biological reactions, comprising: a strip body portion elongated in a longitudinal direction; a plurality of reaction bodies arranged in at least one row at regular intervals on the strip body and having an inlet formed thereon so that at least one analyte can be injected; and a reaction part formed inside the reaction body part and in which a reactant capable of reacting with the analyte may be fixed.

Description

Reaction well strip {Analysis well strip}

The present invention relates to a reaction well strip, and more particularly, to a reaction well strip enabling a biochemical or biological reaction to be smoothly performed.

In recent years, various chemiluminescence (CL) methods have been developed, such as enzyme immunoassay (ELIA), which uses an antigen-antibody reaction, in which chemiluminescent compounds are used as tracers for immunoassays. A chemiluminescence immunoassay (CLIA) used for labeling and a chemiluminescent enzyme immunoassay (CLEIA) that detects enzyme activity with high sensitivity using a chemiluminescent compound in a detection system, etc. there is

In such an immunoassay, the presence and concentration of an analyte are confirmed through biospecific binding of antigens and antibodies. In this process, materials that do not selectively react are removed through washing before signal measurement.

By lowering the background signal through such cleaning, the sensitivity of the signal can be increased.

Capillary-driven flow using capillary force uses a phenomenon in which fluid automatically flows by surface tension without an external pump. Many simple disposable immunoassay products such as pregnancy diagnostic reagents have been developed using such capillary flow. Most of these products use porous materials to induce capillary flow and cannot perform more than two washes.

In addition, US Patent No. 6,271,040 causes capillary flow in a microchannel without using a porous material. However, although the above products and patents use capillary force, there is a problem in that a lot of time and cost are required for analysis because only one sample solution is used to clean the microfluid.

Therefore, there has been an urgent need to develop a technology for a reaction well strip capable of simultaneously analyzing a large amount or multiple types of analytes.

The idea of the present invention is to solve these problems, and it is possible to simultaneously analyze a large amount and a variety of analytes, enable various analyzes by arranging them in M rows and N columns on a tray, and structurally reduce the reaction of the reaction part. It is an object of the present invention to provide a reaction well strip that can be easily attached to and detached from a tray and can be safely and easily analyzed by applying a structure having excellent strength during attachment and detachment.

A reaction well strip according to the spirit of the present invention for solving the above problems includes a strip body portion formed long in a longitudinal direction; a plurality of reaction bodies arranged in at least one row at regular intervals on the strip body and having an inlet formed thereon so that at least one analyte can be injected; and a reaction part formed inside the reaction body part and in which a reactant capable of reacting with the analyte may be fixed.

In addition, according to the present invention, at least one hooking protrusion formed in an arrowhead shape so as to be hooked with the tray on the side or both sides of the strip body part; may further include.

Further, according to the present invention, a reinforcing rib extending from the strip body portion to reinforce strength and formed between the reaction body portion and another neighboring reaction body portion may be further included.

In addition, according to the present invention, the reinforcing ribs, at least one intermediate rib formed in the middle of the strip body portion; and at least one end rib formed at both ends of the strip body portion, wherein a first length of the middle rib may be longer than a second length of the end rib.

In addition, according to the present invention, the diameter of the inlet is larger than the area of the bottom portion, and the reaction portion may include a funnel-shaped inclined surface as a whole so that the diameter continuously decreases from the inlet to the bottom portion.

In addition, according to the present invention, the reaction unit may be formed with radial protrusions formed in a radial shape around the bottom portion on the inclined surface so as to widen the surface area of the reaction surface.

In addition, according to the present invention, the reflective protrusion portion, a first inclined surface portion formed at a first angle relative to the horizontal plane in contact with the bottom portion; a second inclined surface portion connected to the first inclined surface portion and formed at a second angle with respect to the horizontal plane; and a third inclined surface portion connected to the second inclined surface portion and formed at a third angle with respect to the horizontal plane to be in contact with the inlet, and to facilitate accommodating the analysis object through the bottom portion. The angle may be greater than the second angle, and the second angle may be greater than the third angle.

Further, according to the present invention, the analyte may be a biological sample or antigen, and the reactant may be a binding inducing substance or antibody capable of inducing biospecific binding.

In addition, according to the present invention, the reaction body unit is inserted into the cartridge, the inlet is formed in the upper portion to facilitate dispensing of the analysis object from the dispenser through the inlet, and the upper body is formed in a cylindrical shape as a whole; and a lower body entirely formed in a funnel shape below the upper body to facilitate accommodating the analyte through the bottom portion.

According to some embodiments of the present invention made as described above, it is possible to simultaneously analyze a large amount and a variety of analytes, arrange them in M rows and N columns in a tray to enable various analyses, and structurally react the reaction part. can be promoted, and at the same time, it is easy to attach and detach from the tray, and at the same time, it has the effect of safely and easily performing analysis by applying a structure with excellent strength during attachment and detachment. Of course, the scope of the present invention is not limited by these effects.

1 is a perspective view showing a reaction well strip in use according to some embodiments of the present invention.
FIG. 2 is an exterior perspective view illustrating the reaction well strip of FIG. 1 .
FIG. 3 is a partially cut-away perspective view illustrating the reaction well strip of FIG. 2 .
FIG. 4 is a cross-sectional view illustrating the reaction well strip of FIG. 3 .
FIG. 5 is an enlarged cross-sectional view of a reaction part of the reaction well strip of FIG. 4 .
6 is a side view illustrating the reaction well strip of FIG. 4 .
FIG. 7 is a perspective view illustrating a hooking protrusion of the reaction well strip of FIG. 4 .

Hereinafter, various embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art, and the following examples may be modified in many different forms, and the scope of the present invention is as follows It is not limited to the examples. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the spirit of the invention to those skilled in the art. In addition, the thickness or size of each layer in the drawings is exaggerated for convenience and clarity of explanation.

Terms used in this specification are used to describe specific embodiments and are not intended to limit the present invention. As used herein, the singular form may include the plural form unless the context clearly indicates otherwise. Also, when used herein, "comprise" and/or "comprising" specifies the presence of the recited shapes, numbers, steps, operations, elements, elements, and/or groups thereof. and does not exclude the presence or addition of one or more other shapes, numbers, operations, elements, elements and/or groups.

Hereinafter, embodiments of the present invention will be described with reference to drawings schematically showing ideal embodiments of the present invention. In the drawings, variations of the depicted shape may be expected, depending on, for example, manufacturing techniques and/or tolerances. Therefore, embodiments of the inventive concept should not be construed as being limited to the specific shape of the region shown in this specification, but should include, for example, a change in shape caused by manufacturing.

Hereinafter, a reaction well strip according to various embodiments of the present invention will be described in detail with reference to the drawings.

1 is a perspective view showing a reaction well strip 100 in use according to some embodiments of the present invention, FIG. 2 is an external perspective view showing the reaction well strip 100 of FIG. 1, and FIG. 3 is the reaction of FIG. 2 It is a partially cut perspective view showing the well strip 100, and FIG. 4 is a cross-sectional view showing the reaction well strip of FIG.

First, as shown in FIGS. 1 to 4 , the reaction well strip 100 according to some embodiments of the present invention includes a strip body part 110, a reaction body part 10, and a reaction part 20. ) may be included.

For example, as shown in FIGS. 1 to 4 , the strip body portion 110 is coupled to the tray TR and may be a thin rod-shaped structure that is formed long in the longitudinal direction.

In addition, for example, as shown in FIGS. 1 to 4, a plurality of reaction body parts 10 (eight in the drawing) form at least one row and are arranged on the strip body part 110 at regular intervals. It may be a kind of tubular structure in which an inlet 10a is formed on the upper side and a bottom portion 10b is formed on the lower side so that at least one analyte 1 can be injected.

As shown in FIGS. 1 to 4, the reaction body portion 10 may be integrally formed with, for example, the strip body portion 110 and the reaction portion 20 to be described later, It may be made of a synthetic resin material such as plastic or polymer material that does not affect the reaction or has a small effect.

FIG. 7 is a perspective view illustrating a hooking protrusion of the reaction well strip of FIG. 4 .

In addition, for example, as shown in FIGS. 1 to 4 and 7 , the reaction well strip 100 according to some embodiments of the present invention includes trays (TR) on the side or both sides of the strip body 110 . ) and at least one hooking protrusion 111 formed in an arrowhead shape to be engaged with, and other reaction body parts extending from the strip body part 110 to reinforce strength and adjacent to the reaction body part 10. (10) may further include a reinforcing rib 112 formed between them.

More specifically, for example, as shown in FIGS. 1 to 4, the reinforcing rib 112 includes at least one intermediate rib 112a formed in the middle of the strip body portion 110 and the strip At least one end rib 112b formed at both ends of the body portion 110 may be included.

Here, when the strip body portion 110 is forcibly separated by lifting the strip body portion 110 from the tray TR of FIG. 1, the middle portion of the strip body portion 110 where external force is concentrated can be structurally reinforced. The first length L1 of the rib 112a may be longer than the second length L2 of the end rib 112b.

Therefore, as shown in FIGS. 1 to 4 , since a plurality of reaction well strips 100 according to some embodiments of the present invention are prepared and can be easily attached to and detached from the tray TR, a large amount and a variety of The analyte 1 can be analyzed simultaneously, and various analyzes can be made possible by arranging the objects to be analyzed in M rows and N columns on the tray TR, the reaction of the reaction part can be structurally accelerated, and the tray can be easily detached from the tray. At the same time, analysis can be performed safely and easily by applying a structure with excellent strength during attachment and detachment.

FIG. 5 is an enlarged cross-sectional view of the reaction unit 20 of the reaction well strip 100 of FIG. 4 , and FIG. 6 is a side view of the reaction well strip 100 of FIG. 4 .

As shown in FIGS. 1 to 6, more specifically, for example, the reaction body 10 is inserted into the cartridge and passed through the inlet 10a. The inlet 10a is formed at the top to facilitate dispensing of the analysis object 1 from the cycle, and the analysis object 1 is accommodated through the upper body 11 and the bottom portion formed in a cylindrical shape as a whole. For ease of use, a lower body 12 formed in a funnel shape as a whole may be included below the upper body 11 . Of course, the upper body 11 and the lower body 12 may be integrally formed.

Here, as shown in FIGS. 1 to 6, the dispensing of the analyte 1 is performed quickly and after a sufficient reaction time, the inlet 10a can be smoothly accommodated. The diameter D1 of ) may be greater than the area of the bottom portion 10b.

Therefore, the analyte 1 may be introduced into the body 10 through the inlet 10a, and after a certain reaction time, the analyte 1 may be accommodated in the bottom part 10b and the reaction may be smoothly performed. .

In addition, for example, as shown in FIGS. 1 to 6, the reaction part 20 is a part formed inside the reaction body part 10 and can react with the analyte 1. It may be a part to which the sieve 3 can be fixed.

More specifically, for example, the reaction part 20 may include a funnel-shaped inclined surface 21 as a whole so that the diameter continuously decreases from the inlet 10a to the bottom part 10b.

As a result of repeated simulations and experiments, the angle of the inclined surface 21 may be excellent in drainage and wettability when it has a range of about 20 degrees to 80 degrees.

Here, in the reaction part 20, a plurality of radial protrusions T formed in a radial shape around the bottom part 10b may be formed on the inclined surface 21 so as to widen the surface area of the reaction surface. .

As shown in FIG. 3, 9 of the radial protrusions T are arranged in an equal angle, but in addition, at least 3 to 13 may be formed. Here, in the case of two or less, the effect of increasing the surface area is reduced, and in the case of 14 or more, wettability may be deteriorated.

More specifically, for example, as shown in FIGS. 1 to 6, the reflective protrusion T is formed at a first angle K1 with respect to the horizontal plane in contact with the bottom portion 10b. An inclined surface part T1, a second inclined surface part T2 connected to the first inclined surface part T1 and formed at a second angle K2 with respect to the horizontal plane, and the second inclined surface part T2 It may include a third inclined surface part T3 connected to the inlet 10a and formed at a third angle K3 with respect to the horizontal plane.

Here, the first angle K1 is greater than the second angle K2 so that the unreacted analyte 1 is easily discharged through the bottom portion 10b, and the second angle K2 is It may be greater than the third angle K3.

For example, as a result of repeated simulations and experiments, the first angle may be approximately 80 to 90 degrees, the second angle may be approximately 60 to 80 degrees, and the third angle may be approximately 40 to 60 degrees. and excellent in wettability.

In addition, for example, the analyte 1 may be a biological sample or antigen, and the reactant 3 may be a binding inducing substance or antibody capable of inducing biospecific binding.

Therefore, when describing a specific method of using the reactive well strip 100 according to some embodiments of the present invention, a binding inducer capable of adsorption or biospecific binding within the reactive well strip 100 can be IL. -6 Specific antibody is selected and fixed to the surface. For example, a 30uL sample solution of 1% glutaraldehyde diluted with 0.1M MES (pH6.1) is used to immobilize the antibody for 30 minutes. can

Subsequently, it may be washed once for 10 minutes using 30uL of a cleaning solution composed of 0.1M MES (pH 6.1). A reaction between glutaaldehyde and IL-6 antibody can be induced by adding 10 μg/mL or 30 μL of an IL-6 specific antibody for 30 minutes.

Then, 2% BSA, 0.05% TritonX100-0.1M Tris (pH7.6) sample solution was added and reacted twice for 10 minutes. ) can be removed.

Subsequently, 30 μL of IL-6 antigen was added for each concentration to be analyzed, and binding with the antibody was allowed to occur for 30 minutes. 30uL of cleaning solution consisting of 0.05% TritonX100-Tris (pH7.6) can be added and washed once for 10 minutes.

Subsequently, 2ug/mL and 30uL of antibody-biotin polymer were added and biospecific binding was induced for 30 minutes. After adding 30uL of cleaning solution consisting of 0.05% TritonX100-Tris (pH7.6), it can be cleaned once for 10 minutes.

Then, 30 uL of streptavidin-HRP polymer is then added and biospecific binding between the antibody-biotin polymer and the streptavidin-enzyme polymer can be allowed to occur for 30 minutes.

Subsequently, 30 uL of a washing solution composed of 0.05% TritonX100-Tris (pH 7.6) was added and washed three times at 10-minute intervals to wash away unbound streptavidin-enzyme.

Finally, 30uL of a substrate that reacts with the enzyme is added, reacted for 15 minutes, and the amount of IL-6 antigen can be quantified. At this time, analysis methods such as absorption, fluorescence, and chemiluminescence may be determined according to the type of substrate reacting with the enzyme.

Although the present invention has been described with reference to the embodiments shown in the drawings, this is merely exemplary, and those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. Therefore, the true technical protection scope of the present invention should be determined by the technical spirit of the appended claims.

TR: tray
1: analysis object
2: unreacted analyte
3: reactant
10: reaction body
10a: inlet
10b: bottom part
11: upper body
12: lower body
20: reaction unit
21: slope
T: Radial protrusion
T1: first slope portion
T2: second slope portion
T3: third slope portion
K1: first angle
K2: second angle
K3: 3rd angle
100: reaction well strip
110: strip body
111: locking protrusion
112: reinforcing rib
112a: middle rib
112b: end rib
L1: first length
L2: second length

Claims (9)

A strip body portion formed long in the longitudinal direction;
a plurality of reaction bodies arranged in at least one row at regular intervals on the strip body and having an inlet formed thereon so that at least one analyte can be injected; and
a reaction part formed inside the reaction body part and in which a reactant capable of reacting with the analyte may be fixed;
including,
At least one hooking protrusion formed in an arrowhead shape to be hooked with the tray on the side or both sides of the strip body;
Including more,
reinforcing ribs extending from the strip body to reinforce strength and formed between the reaction body and another neighboring reaction body;
and more
The reinforcing ribs,
at least one intermediate rib formed in an intermediate portion of the strip body portion; and
at least one end rib formed at both ends of the strip body;
including,
wherein the first length of the middle rib is longer than the second length of the end rib. delete delete delete According to claim 1,
The diameter of the inlet is larger than the area of the bottom,
The reaction part,
A reaction well strip comprising a funnel-shaped inclined surface as a whole to continuously decrease in diameter from the inlet to the bottom. According to claim 5,
Wherein the reaction part comprises radial protrusions formed in a radial shape around the bottom part on the inclined surface so as to increase a surface area of the reaction surface. According to claim 6,
The reflective protrusion,
a first inclined surface portion formed at a first angle with respect to a horizontal plane in contact with the bottom portion;
a second inclined surface portion connected to the first inclined surface portion and formed at a second angle with respect to the horizontal plane; and
A third inclined surface portion connected to the second inclined surface portion and formed at a third angle with respect to the horizontal plane to be in contact with the inlet; includes,
wherein the first angle is greater than the second angle and the second angle is greater than the third angle so as to facilitate accommodating the analyte through the bottom portion. According to claim 1,
Wherein the analyte is a biological sample or antigen, and the reactant is a binding inducing substance or antibody capable of inducing biospecific binding. According to claim 1,
The reaction body part,
an upper body which is inserted into the cartridge, has the inlet formed thereon to facilitate dispensing of the analyte from the dispenser through the inlet, and is generally formed in a cylindrical shape; and
a lower body entirely formed in a funnel shape below the upper body to facilitate accommodating the analyte through the bottom portion;
A reaction well strip comprising a.

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