KR20220058195A - Soybean variety with excellent adaptability to highland and semi-highland regions - Google Patents

Abstract

The present invention relates to a novel soybean variety with improved yield due to excellent adaptability to highland and semi-highland regions and a growing method thereof. A soybean variety produced by the present invention has improved coloration and excellent adaptability to highland and semi-highland regions compared to the existing variety, and is expected to have high utilization value.

Description

Soybean variety with excellent adaptability to highland and semi-highland regions

The present invention relates to a novel soybean line with improved yield due to excellent adaptability in high and semi-alpine regions and a breeding method thereof.

In highland agriculture, which is centered on Daegwallyeong, major short-term crops such as potatoes, Chinese cabbage, and radishes are grown in series. As a result, the soil environment deteriorates, pests and pests multiply, and as a result, the yield decreases, so the vicious cycle of over-injecting pesticides and fertilizers is repeated. In addition, since most of the vegetable cultivation areas in highland regions are located upstream of the water system, various nutrients such as lost soil, nitrogen, and phosphoric acid flow into the river, which is a major cause of environmental problems. To solve this problem, the development of alternative crops that can dramatically reduce the environmental burden while guaranteeing the profitability of existing crops is emerging as a major task in high-altitude agricultural research.

Soybeans are economically important crops around the world, and they are a raw material for traditional foods such as soybean paste and soy sauce eaten by most Koreans every day. Soybean is an eco-friendly crop that grows well even with little fertilizer, and is a very important crop suitable for planting sequence (cropping system) with other crops. In addition, as the effect of useful ingredients has been highlighted, the demand for soybeans as a well-being food of consumers who are sensitive to health functions is increasing. Soybean seeds (seeds) contain 40% of protein, 30% of carbohydrate, and 20% of fat, which is the most important of the three macronutrients required for humans. While there is almost no starch, it has a lot of protein, so it is a crop with superior nutritional value than any other crops on the ground. Soybeans contain most of the vitamins, especially B vitamins (thiamine, riboflavin, and nicotinic acid). On the other hand, the value of vitamin C is recognized in bean sprouts and green beans, and as inorganic components, calcium, phosphorus, iron, and potassium are much higher than that of beef, so beans have been called 'field beef' since ancient times.

These beans are considered to be optimal crops to improve the fertility of high altitudes deteriorated due to continuous cultivation because they fix nitrogen in the atmosphere through a symbiotic relationship with soil microorganisms. However, as described above, soybeans are environmentally friendly, have excellent food value, and are of great importance in high-altitude agriculture. Research and development in Korea is still insufficient (Korean J. Crop Sci., 2008, 53(1): 102-109).

Accordingly, the present inventors have improved cultivation stability, yield and functionality, but improved 'Daepung' soybeans, which yield decreased due to a lower ripening rate when cultivated in high and semi-high altitudes compared to in flatlands, and adaptability in high and semi high altitudes. An attempt was made to obtain this improved new breeding material.

The present inventors have made research efforts to develop a breeding material with improved adaptability in high-altitude and semi-high-altitude areas and improved coloration by using Daepung soybean, which has various advantages. The 10 lines were established, and the present invention was completed based on this.

Accordingly, an object of the present invention is to provide a plant with improved yield in high or semi-alpine regions deposited at the Agricultural Genetic Resource Center of the National Academy of Agricultural Sciences.

Another object of the present invention is to provide seeds and progeny of the seeds according to the plants of the above lineage.

Another object of the present invention is to provide a method for breeding plants of the above lineage.

However, the technical problem to be achieved by the present invention is not limited to the above-mentioned problems, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to achieve the object of the present invention as described above, the present invention provides a plant with improved yield in high or semi-alpine regions selected from the group consisting of the following accession numbers deposited with the Agricultural Genetic Resources Center of the National Academy of Agricultural Sciences. .

Plants of line HD-F13-117 deposited with accession number KACC 98088P;

Plants of line HD-F13-121 deposited with accession number KACC 98089P;

Plants of line HD-F13-132 deposited with accession number KACC 98090P;

Plants of line HD-F13-160 deposited with accession number KACC 98091P;

Plants of line HD-F13-194 deposited with accession number KACC 98092P;

Plants of line HD-F13-202 deposited with accession number KACC 98093P;

Plants of line HD-F13-211 deposited with accession number KACC 98094P;

Plants of line HD-F13-215 deposited with accession number KACC 98095P;

Plants of line HD-F13-236 deposited with accession number KACC 98097P; and

Plants of line HD-F13-264 deposited with accession number KACC 98096P.

In one embodiment of the present invention, the plant lineage may have improved yield by 20% or more in high altitudes or semi high altitudes.

In another embodiment of the present invention, the plant lineage may be a mid-life species or a mid-late species.

In another embodiment of the present invention, the plant lineage may have a weight of 20 to 32 g of white grains.

In another embodiment of the present invention, plants of lineages other than HD-F13-236 may have a yellow color.

In another embodiment of the present invention, the plant lineage may have an average length of 60 to 120 cm, and an average number of narrows of 55 to 100.

In addition, the present invention provides a seed according to the above lineage plant.

The present invention also provides progeny of seeds according to the above lineage plants.

In addition, the present invention provides a method for breeding plants of the above lineage comprising the following steps.

(a) cultivating a hybrid line obtained by artificial crossing with golden soybean as a mating model and daepungkong as a mating parent up to the F9 generation; and

(b) selecting the line with improved yield in the high altitude or semi high altitude area after cultivating the cultivated lineage plant in high altitude, semi high altitude, and flat warm area.

According to the present invention, compared to the existing breeding through backcrossing takes more than 6-7 generations, the present invention uses an intermediate specimen, so it is possible to select individuals more quickly, thereby reducing the period of breeding new varieties by 2-3 generations. There is an advantage in that the scale and efficiency of breeding can be increased by reducing the effort and cost required for crop improvement. In addition, the soybean line produced by the present invention has improved color making and excellent adaptability in high and semi-high altitudes compared to Daepung, which is an existing variety, so its utility value is expected to be high.

The present inventors have made research efforts to develop a breeding material with improved adaptability in high-altitude and semi-high-altitude areas and improved coloration by using Daepung soybean, which has various advantages. The 10 lines were established, and the present invention was completed based on this.

Accordingly, the present invention provides a plant with improved yield in high or semi-alpine regions selected from the group consisting of the following accession numbers deposited with the Agricultural Genetic Resource Center of the National Academy of Agricultural Sciences.

Plants of line HD-F13-117 deposited with accession number KACC 98088P;

Plants of line HD-F13-121 deposited with accession number KACC 98089P;

Plants of line HD-F13-132 deposited with accession number KACC 98090P;

Plants of line HD-F13-160 deposited with accession number KACC 98091P;

Plants of line HD-F13-194 deposited with accession number KACC 98092P;

Plants of line HD-F13-202 deposited with accession number KACC 98093P;

Plants of line HD-F13-211 deposited with accession number KACC 98094P;

Plants of line HD-F13-215 deposited with accession number KACC 98095P;

Plants of line HD-F13-236 deposited with accession number KACC 98097P; and

Plants of line HD-F13-264 deposited with accession number KACC 98096P.

In addition, the present invention provides a seed according to the above lineage plant.

The present invention also provides progeny of seeds according to the above lineage plants.

The present inventors have secured the 10 lines through the following breeding method.

More specifically, in one embodiment of the present invention, 620 lines obtained by artificial crossbreeding with 'Golden' cultivar as the parent and 'Daepung' as the secondary were advanced to the F9 generation by the phylogenetic breeding method. After that, the main agricultural traits were evaluated for 4 years (2016-2019) in the fields of the highland (Daegwallyeong), the semi-highland (Jinbu), and the Pyeongnanji (Gangneung), and 10 strains with excellent color and yield were selected (Example 1) reference).

In another embodiment of the present invention, in order to analyze the agricultural traits of the 10 lines, the intrinsic characteristics and general characteristics were compared with the existing cultivar Daepung, and as a result, among the unique characteristics of the selected 10 lines, one view compared to the Daepung Kong. There was a difference in flower color and hair color in , and in the remaining 9 strains except for HD-F13-236, there was a difference in the color of yellow (pale yellow). In addition, while the flowering and maturation phases were similar to those of Daepung beans, the long length was varied from 63 cm to 113 cm, and the number of narrows per individual was 60 to 96, which was higher than that of Daepung (see Examples 2-1 and 2-2). .

In another embodiment of the present invention, as a result of comparing the results of cultivation in the Jinbu region, which is a semi-highland region with the existing variety Daepungkong, the yield of 10 lines according to the present invention is 20% to 67% compared to that of Daepungkong. It was confirmed that the water was increased (see Example 2-3).

The present inventors deposited each of the 10 strains obtained through the above example at the Agricultural Genetic Resource Center of the National Academy of Agricultural Sciences and were given an accession number as of September 25, 2020, and the accession number is as described above.

In the present invention, the plants of each lineage may have improved yield in high altitudes or semi high altitudes.

As used herein, the term “high altitude” refers to an area that is 600 m or higher above sea level based on the altitude, and “quasi-high altitude” refers to an area that is 400 to 600 m above sea level.

The plant of the above lineage according to the present invention is a mid-life or late-end species, and the weight of the white grain may be 20 to 32 g, and preferably 20.9 to 30.8 g, but is not limited thereto.

In the present invention, 9 line plants except for HD-F13-236 may have a yellow color.

As used in the present invention, the term “hilum color” is also called navel color, and is one of the characteristics of beans that affect the appearance quality and processing aptitude of beans, and refers to the color of the eye area of soybean seeds. In the present invention, the yellow color means more preferably pale yellow.

The plant of the lineage according to the present invention may have an average length of 60 to 120 cm, and an average number of narrows may be 55 to 100, preferably, an average length of 63 to 113 cm and an average number of narrows may be 60 to 96, but limited to this it is not

As another aspect of the present invention, the present invention provides a method for breeding a plant of the above line, comprising the following steps.

(a) cultivating a hybrid line obtained by artificial crossing with golden soybean as a mating model and daepungkong as a mating parent up to the F9 generation; and

(b) selecting the line with improved yield in the high altitude or semi high altitude area after cultivating the cultivated lineage plant in high altitude, semi high altitude, and flat warm area.

As used in the present invention, the term “mating parent” refers to the parent used as a mating material to foster excellent offspring in breeding. The "crossing copy" means used as a breeding material having the trait to be introduced, and in the present invention, the Daepungkong variety nurtured by the cross of Baegunkong/Shinpaldalkong No.2 was used.

Hereinafter, preferred examples are presented to help the understanding of the present invention. However, the following examples are only provided for easier understanding of the present invention, and the contents of the present invention are not limited by the following examples.

[Example]

Example 1. Cultivation of soybean lines with excellent adaptability to high altitudes and semi-high altitudes

‘Daepung’ soybeans are a variety with high utility value due to their high functional ingredients, planting stability and yield, but when grown in high and semi-high altitudes, the ripening rate is lowered compared to the plains and the yield decreases. In addition, there is a limitation in that the preference of distributors and consumers is low because daepoong beans have a brown color. Accordingly, the present inventors tried to develop a breeding material with improved color making and adaptability in high and semi-highland areas while maintaining the excellent characteristics of Daepung.

More specifically, for color improvement of Daepung beans, 620 lines obtained by artificial crossbreeding with the ‘Golden’ variety as the parent and ‘Daepung’ as the parent were advanced to the F9 generation by the phylogenetic breeding method. Afterwards, 10 strains with excellent color quality and yield were selected by evaluating major agricultural traits for 4 years (2016-2019) in the fields of the highland (Daegwallyeong), semi-highland (Jinbu), and Pyeongnanji (Gangneung). The main agricultural traits of the 10 selected lines are summarized in Table 1 below.

For reference, the quantity average for 3 years from 2016 to 2018 is presented as a ratio to the quantity of large winds in each region, and the average value among 100 grains for 3 years from 2016 to 2018 is also shown. In addition, the number of days of growth represents the average value of the number of days of growth evaluated in the Jinbu area over the same three years.

genealogy discoloration Quantity (ratio to large winds) In 100 grains (g) number of days of growth Daegwallyeong banality Gangneung Daegwallyeong banality Gangneung big wind Brown 1.00 1.00 1.00 20.94 23.24 23.64 147 days crew yellow 0.84 0.99 0.99 23.63 27.68 28.13 148 days HD-F13-117 yellow 1.14 1.00 1.23 24.99 24.47 25.31 137 days HD-F13-121 yellow 0.64 0.73 0.93 21.24 24.23 24.66 135 days HD-F13-132 yellow 0.87 1.25 1.31 19.76 22.42 22.71 159 days HD-F13-160 yellow 0.89 1.10 1.19 26.14 29.30 32.38 144 days HD-F13-194 yellow 1.54 1.32 1.06 23.16 24.55 23.22 134 days HD-F13-202 yellow 1.09 1.17 1.31 18.18 20.92 21.65 135 days HD-F13-211 yellow 1.30 0.95 1.26 24.25 26.89 26.86 143 days HD-F13-215 yellow 1.27 1.17 1.00 26.85 30.79 27.73 135 days HD-F13-236 Brown 1.85 1.11 1.12 25.02 28.90 28.82 141 days HD-F13-264 yellow 1.09 1.17 1.31 23.58 30.00 29.31 156 days

Example 2. Agricultural trait analysis of existing varieties and breeding lines

The present inventors compared various characteristics with Daepungkong, an existing variety, in order to analyze the agricultural traits of the 10 breeding lines obtained in Example 1.

First, the differences between the breeding line and the existing variety according to the present invention are summarized and shown in Table 2 below, and the comparative analysis results for each characteristic are as follows.

breed name upbringing process flower color belly color Wear side height (cm) Quantity per object (g) In 100 grains (g) Existing varieties
(Daepoong Bean) White Beans/
Shinpaldalkong No.2 White soak 18 25.2 23.3 HD-F13-117 gold / big wind White buffalo 10 37.6 24.5 HD-F13-121 gold / big wind purple buffalo 17 30.3 24.2 HD-F13-132 gold / big wind purple buffalo 22 37.1 22.4 HD-F13-160 gold / big wind purple buffalo 12 38.9 29.3 HD-F13-194 gold / big wind purple buffalo 11 38.9 24.6 HD-F13-202 gold / big wind White buffalo 11 36.6 20.9 HD-F13-211 gold / big wind purple buffalo 10 40.3 26.9 HD-F13-215 gold / big wind White buffalo 12 30.5 30.8 HD-F13-236 gold / big wind White soak 11 42.2 28.9 HD-F13-264 gold / big wind White buffalo 9 36.7 30.0

2-1. Comparison of unique characteristics

The intrinsic characteristics between the breeding line and the existing variety were compared, and the results are shown in Table 3 below. More specifically, the intrinsic characteristics of the 10 selected lines are finite elongation, the leaf shape is ovate, and the shape of the seed is oval. The flower color is white (117, 202, 215, 236, 264) and purple (121, 132, 160, 194, 211), and in the case of color (navel color), the remaining 9 strains are pale yellow except for HD-F13-236. It was confirmed that there was a clear difference from the light brown daepung beans.

breed name new form flower color leaf type hair color seed color seed shape discoloration detachment soybean finite White ovoid gray yellow rectangle soak I HD-F13-117 finite White ovoid Brown yellow rectangle buffalo I HD-F13-121 finite purple ovoid gray yellow rectangle buffalo I HD-F13-132 finite purple ovoid gray yellow rectangle buffalo I HD-F13-160 finite purple ovoid Brown yellow rectangle buffalo I HD-F13-194 finite purple ovoid Brown yellow rectangle buffalo I HD-F13-202 finite White ovoid Brown yellow rectangle buffalo I HD-F13-211 finite purple ovoid Brown yellow rectangle buffalo I HD-F13-215 finite White ovoid Brown yellow rectangle buffalo I HD-F13-236 finite White ovoid Brown yellow rectangle soak I HD-F13-264 finite White ovoid Brown yellow rectangle buffalo I

2-2. Comparison of general characteristics

In order to compare the general characteristics between the breeding line and the existing variety, the results performed in Jinbu-myeon, Pyeongchang-gun, Gangwon-do, sown on June 13, 2019, are summarized and shown in Table 4 below. As a result, it was confirmed that the flowering and maturation periods of the 10 selected lines were mid-to-mid-late species like Daepungkong, a comparative variety, and the length was 63cm (HD-F13-215) to 113cm (HD-F13-132). . The number of narrows per individual was 60 (HD-F13-215) to 96 (HD-F13-132), and it was found that all 10 lines were higher than the Daepung.

Flowering period (Month) Maturity (Month) Length (cm) uniform Daylight Savings narrow water (dog) Wear side height (cm) soybean 7.30. 10.19. 68 River 14 51 18 HD-F13-117 7.26. 10.11. 78 River 14 74 10 HD-F13-121 7.29. 10.18. 89 middle 16 72 17 HD-F13-132 8.10. 10.21. 113 River 18 96 22 HD-F13-160 7.27. 10.18. 92 River 16 62 12 HD-F13-194 7.29. 10.16. 65 River 14 85 11 HD-F13-202 7.30. 10.18. 90 River 16 86 11 HD-F13-211 7.31. 10.18. 81 approximately 13 75 10 HD-F13-215 7.27. 10.11. 63 River 12 60 12 HD-F13-236 7.30. 10.19. 77 middle 13 86 11 HD-F13-264 7.30. 10.21. 79 River 12 66 9

2-3. Quantity comparison

Finally, the yield was compared between the breeding line and the existing variety, and the results are shown in Table 5 below. As a result of the analysis, it was confirmed that the yield of the 10 strains selected in the present invention was increased by 20% (HD-F13-121) to 67% (HD-F13-236) compared to Daepung beans during vinyl mulching cultivation in Jinbu in 2019. In addition, in 100 grains, it was found that 20.9g (HD-F13-202) to 30.8g (HD-F13-215) were present in various ways from neutral to allele.

Quantity per object (g) Indices(%) In 100 grains (g) Indices(%) soybean 25.2 100 23.3 100 HD-F13-117 37.6 149 24.5 105 HD-F13-121 30.3 120 24.2 104 HD-F13-132 37.1 147 22.4 96 HD-F13-160 38.9 154 29.3 126 HD-F13-194 38.9 154 24.6 106 HD-F13-202 36.6 145 20.9 90 HD-F13-211 40.3 160 26.9 115 HD-F13-215 30.5 121 30.8 132 HD-F13-236 42.2 167 28.9 124 HD-F13-264 36.7 146 30.0 129

The description of the present invention stated above is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. There will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Agricultural Genetic Resource Center, National Academy of Agricultural Sciences KACC98088P 20200925 Agricultural Genetic Resource Center, National Academy of Agricultural Sciences KACC98089P 20200925 Agricultural Genetic Resource Center, National Academy of Agricultural Sciences KACC98090P 20200925 Agricultural Genetic Resource Center, National Academy of Agricultural Sciences KACC98091P 20200925 Agricultural Genetic Resource Center, National Academy of Agricultural Sciences KACC98092P 20200925 Agricultural Genetic Resource Center, National Academy of Agricultural Sciences KACC98093P 20200925 Agricultural Genetic Resource Center, National Academy of Agricultural Sciences KACC98094P 20200925 Agricultural Genetic Resource Center, National Academy of Agricultural Sciences KACC98095P 20200925 Agricultural Genetic Resource Center, National Academy of Agricultural Sciences KACC98097P 20200925 Agricultural Genetic Resource Center, National Academy of Agricultural Sciences KACC98096P 20200925

Claims (9)

Plants with improved yield in high or semi-alpine regions selected from the group consisting of the following accession numbers deposited at the Agricultural Genetic Resource Center of the National Academy of Agricultural Sciences:
Plants of line HD-F13-117 deposited with accession number KACC 98088P;
Plants of line HD-F13-121 deposited with accession number KACC 98089P;
Plants of line HD-F13-132 deposited with accession number KACC 98090P;
Plants of line HD-F13-160 deposited with accession number KACC 98091P;
Plants of line HD-F13-194 deposited with accession number KACC 98092P;
Plants of line HD-F13-202 deposited with accession number KACC 98093P;
Plants of line HD-F13-211 deposited with accession number KACC 98094P;
Plants of line HD-F13-215 deposited with accession number KACC 98095P;
Plants of line HD-F13-236 deposited with accession number KACC 98097P; and
Plants of line HD-F13-264 deposited with accession number KACC 98096P.
The method of claim 1,
The plant lineage is characterized in that the yield is increased by 20% or more in high altitudes or semi high altitudes.
The method of claim 1,
The plant lineage is a plant, characterized in that it is a mid-life or mid-late species.
The method of claim 1,
The plant of the lineage is characterized in that 20 to 32 g of white grains, plants.
The method of claim 1,
Plants except for the HD-F13-236 are characterized in that the color is yellow.
The method of claim 1,
The plant lineage has an average length of 60 to 120 cm, and an average narrow number of 55 to 100, plants.
A seed according to any one of claims 1 to 6 of the line plant.
The progeny of a seed according to any one of claims 1 to 6 of the lineage plant.
A method for breeding plants according to claim 1, comprising the steps of:
(a) cultivating a hybrid line obtained by artificial crossing with golden soybean as a mating model and daepungkong as a mating parent up to the F9 generation; and
(b) selecting a line with improved yield in a high altitude or semi high altitude area after developing and cultivating the cultivated plant line in a high altitude, semi high altitude, and flat warm area.