KR20220043015A - Biosensor structure for specimen measurement and mehtod for measuring specimen using the same - Google Patents

Abstract

Disclosed are a biosensor structure for sample measurement and a sample measurement method using the same. In the biosensor electrode structure for sample measurement, the biosensor electrode structure according to an embodiment of the present invention is characterized in that a working electrode and a reference electrode, which are electrodes for measuring a sample, are disposed spaced apart from each other in the longitudinal direction of a sample insertion passage. In a portion corresponding to the sample insertion passage, a working protrusion, which is a protrusion of the working electrode, and reference protrusions, which are two protrusions of the reference electrode, are alternately arranged, and the ratio of the area of the working protrusion to the area of the reference protrusions is 1 or more. At least one recognition electrode for recognizing the sample has a structure disposed adjacent to the working electrode or the reference electrode and spaced apart in parallel with the working electrode and the reference electrode. In the at least one recognition electrode, at least one recognition protrusion, which is a protrusion, is disposed at a portion corresponding to the sample insertion passage. Accordingly, measurement accuracy for substances included in the sample can be improved.

Description

Biosensor structure for sample measurement and sample measurement method using the same

The present invention relates to a biosensor structure for sample measurement and a sample measurement technology using the same, and more particularly, to a biosensor structure capable of improving the measurement accuracy for glucose contained in a sample, for example, saliva, and It relates to a sample measurement method using the same.

Quantitative or qualitative analysis of analytes present in a bio sample is chemically and clinically important. For example, measuring blood sugar in blood for diabetic patients or measuring cholesterol, which is a factor in various adult diseases, is a representative example.

As is known in the art, an electrochemical biosensor using the activity of an enzyme is a specific substance in a biological sample (hereinafter, referred to as a 'sample'), for example, saliva or blood, such as glucose in clinical chemistry. In sensor, uric acid sensor, protein sensor, DNA sensor, shicross sensor, liver function test, it is very important to measure enzyme activity such as GOT (Glutamate-Oxaloacetate Transaminase) or GPT (Glutamate-Pyruvate Transaminase) more quickly and reproducibly. . Here, the biosensor is divided into an identification part that identifies a measurement target and a change part that converts it into an electrical signal.

A biological material is used for the identification part, and when the biological material recognizes the measurement target, a chemical change or a physical change occurs. A site that converts such a change into an electrical signal is a change site, and the identification site and the change site are collectively called a biosensor electrode.

The currently commercialized strip-type biosensor measurement method uses the capillary phenomenon, a force stronger than the earth's gravity, which is made possible through plasma or chemical surfactant treatment during the manufacturing process, and introduces a sample into the sample insertion path of the biosensor. The general method is to measure the sample qualitatively or quantitatively after accumulating it in the insertion channel.

In this case, in general, prior to sample measurement, a step of detecting the point of introduction of the sample, which is the point at which the sample is introduced and accumulated in the sample insertion passage, is performed.

In the conventional case, the sample inflow detection signal is applied to the working electrode and the reference electrode of the biosensor to detect the sample inflow time, and after a predetermined time has elapsed, the sample measurement signal is applied to the working electrode and the reference electrode to measure the sample. will be used That is, starting from the time when the sample arrives at the working electrode and the reference electrode, the time point at which the sample is inserted and completely reached the flow path volume formed is calculated in time to determine the sample inflow time. The sample is measured by applying the sample measurement signal to the and the reference electrode.

In this case, an electric double layer (EDL) is formed because the sample inflow detection signal applied to detect the sample inflow point already reacts on the surfaces of the working electrode and the reference electrode, which are important as the measuring electrode. . Here, the electric double layer appears at the boundary between different materials (electrodes, samples, or solutions), and occurs when an electric field is applied to the contact surfaces of different materials. Although the electric double layer capacitance (DLC), which is the capacitance of the electric double layer, is insignificant, it may be included in the current signal measured when the sample detection signal is applied. Accordingly, the measurement signal of the biosensor may be distorted and the measurement result may be affected.

In addition, the measurement after waiting for an arbitrary time after calculating the time from the point of introduction of the sample to the accumulation of the sample in the flow path will be insignificant since the viscosity of each sample is different, but it depends on the speed or time at which the sample is accumulated in the flow path. They may be different and affect the measurement results. Therefore, determining the time of application of the sample measurement signal using temporal control may cause significant problems in measurement accuracy.

Embodiments of the present invention provide a biosensor structure capable of improving measurement accuracy for glucose contained in a sample, for example, saliva, and a method for measuring a sample using the same.

In the embodiments of the present invention, the measurement accuracy of the material contained in the sample can be improved by collecting the sample, removing the interfering material that interferes with the sample measurement from the collected sample, and then measuring the sample through the biosensor. A sample measurement system is provided.

The biosensor electrode structure according to an embodiment of the present invention is a biosensor electrode structure for measuring a sample. are spaced apart from each other in the direction, and a working protrusion that is a protrusion of the working electrode and a reference protrusion that are two protrusions of the reference electrode are alternately arranged in a portion corresponding to the sample insertion passage, and the area of the working protrusion and the The area ratio is greater than or equal to 1, and at least one recognition electrode for recognizing the sample is adjacent to the working electrode or the reference electrode and has a structure in which the working electrode and the reference electrode are spaced apart from each other and spaced apart from the working electrode. The recognition electrode is characterized in that the recognition protrusion, which is at least one protrusion, is disposed on a portion corresponding to the sample insertion passage.

The recognition protrusion may be disposed adjacent to the distal end of the sample insertion passage.

The at least one recognition electrode may perform sample recognition in response to a sample recognition signal applied independently of the sample measurement signal applied to the working electrode and the reference electrode.

Furthermore, the biosensor electrode structure according to an embodiment of the present invention may further include a strip recognition electrode for detecting a moment when the biosensor having the electrode structure of the biosensor is inserted into the measuring device for measurement.

The biosensor structure according to an embodiment of the present invention recognizes a working electrode and a reference electrode, which are electrodes for measuring a sample on an insulating substrate, and a reference electrode, and the sample in the biosensor structure for measuring a sample at least one recognition electrode is disposed for The reference projections, which are two projections of the reference electrode, are alternately arranged, the area of the working projection and the area ratio of the reference projections is 1 or more, and the at least one recognition electrode is adjacent to the working electrode or the reference electrode and is adjacent to the working electrode. and a lower plate having a structure spaced apart from the reference electrode and having a structure in which at least one recognition protrusion, which is a protrusion, is disposed in a portion corresponding to the sample insertion passage. a middle plate having the sample insertion passage formed therein, and into which an enzyme compound is inserted; and an upper plate in which an insertion hole for inserting the sample and an outlet for discharging air are formed in a portion of the insulating substrate corresponding to the sample insertion passage.

The enzyme compound is an enzyme for selectively reacting glucose contained in the saliva when the sample is saliva, and attaching the enzyme to the electrodes disposed on the lower plate. a polymer and a catalyst for accelerating the reaction between the enzyme and the glucose, wherein the enzyme contains at least one of glucose oxidase and glucose dehydrogenase (GDH) in 1 unit ~ 30 units, wherein the polymer contains at least one of Chitosan, PVP, Nafion, polyethylene glycol, poly vinyl pyrrolidone, poly vinyl alcohol, agarose and trehalose by wt 0.01% to 1.0%, and the catalyst may include 1% to 10% of at least one of ferrocene, ferrocene derivative, quinone, quinone derivative, hexaamine ruthenium (III) chloride and ferricyanide.

The catalyst may include 0.001% to 5% of the Prussian blue.

The at least one recognition electrode may perform sample recognition in response to a sample recognition signal applied independently of the sample measurement signal applied to the working electrode and the reference electrode.

A sample measurement system according to an embodiment of the present invention comprises: a sampling device for collecting a sample and a filter for removing an interfering substance that interferes with measuring the sample from the sample collected by the sampling means; A working electrode and a reference electrode, which are electrodes for measuring the sample from which the interference material has been removed, and at least one recognition electrode for recognizing the sample from which the interference material has been removed are disposed, the working electrode and the reference electrode are arranged to be spaced apart from each other in the longitudinal direction of the sample insertion flow path, and in a portion corresponding to the sample insertion flow path, a working protrusion, which is a protrusion of the working electrode, and a reference protrusion, which is two protrusions of the reference electrode, are alternately arranged, The ratio of the area of the working protrusion to the area of the reference protrusions is equal to or greater than 1, and the at least one recognition electrode is adjacent to the working electrode or the reference electrode and has a structure in which the working electrode and the reference electrode are spaced apart from each other in parallel. , a biosensor having an electrode structure in which a recognition protrusion, which is at least one protrusion, is disposed on a portion corresponding to the sample insertion passage; and after the strip of the biosensor is inserted, when the sample from which the interference material has been removed through the collection device is inserted into the biosensor, the interference material is generated based on the sample measurement signal received through the working electrode and the reference electrode. A measuring device for measuring the removed sample is included.

The filter removes large molecules and foreign substances present in the saliva when the sample is saliva, and separates substances including proteins, amylases and ions that interfere with measuring sugar in the saliva can do.

The biosensor includes a lower plate having the electrode structure; a middle plate having the sample insertion passage formed therein and into which an enzyme compound is inserted; and an upper plate in which an insertion hole for inserting the sample from which the interference material has been removed and an outlet for discharging air are formed in a portion corresponding to the sample insertion passage.

The enzyme compound is an enzyme for selectively reacting glucose contained in the saliva when the sample is saliva, and attaching the enzyme to the electrodes disposed on the lower plate. a polymer and a catalyst for accelerating the reaction between the enzyme and the glucose, wherein the enzyme contains at least one of glucose oxidase and glucose dehydrogenase (GDH) in 1 unit ~ 30uni, wherein the polymer contains at least one of Chitosan, PVP, Nafion, polyethylene glycol, poly vinyl Pyrrolidone, poly vinyl alcohol, agarose and trehalose by wt 0.01% to 1.0%, the catalyst may include 1% to 10% of at least one of ferrocene, ferrocene derivative, quinone, quinone derivative, hexaamine ruthenium (III) chloride and ferricyanide.

The catalyst may include 0.001% to 5% of the Prussian blue.

A method for measuring a sample according to an embodiment of the present invention includes the steps of collecting a sample using a sampling means and removing an interfering substance that interferes with measuring the sample from the sampled sample using a filter; contacting the biosensor with the sample from which the interference material has been removed; and when the contact of the sample from which the interference material has been removed is recognized by at least one recognition electrode of the biosensor, a sample measurement signal is applied to a working electrode and a reference electrode of the biosensor to determine the interference. measuring a response signal to the sample from which the material has been removed, wherein the working electrode and the reference electrode are spaced apart from each other in the longitudinal direction of the sample insertion flow path, and the working electrode in a portion corresponding to the sample insertion flow path A working protrusion that is a protrusion of the reference electrode and a reference protrusion that are two protrusions of the reference electrode are alternately arranged, an area ratio of the working protrusion to an area ratio of the reference protrusions is 1 or more, and the at least one recognition electrode is the working electrode or the reference electrode It has a structure in which it is adjacent to and is spaced apart from the working electrode and the reference electrode in parallel, and at least one recognition protrusion, which is a protrusion, may be disposed in a portion corresponding to the sample insertion passage.

According to embodiments of the present invention, by collecting a sample, for example, saliva, removing an interfering material that interferes with sample measurement from the sampled sample, and then measuring glucose contained in saliva through a biosensor, It is possible to improve the measurement accuracy of substances included in the sample.

According to embodiments of the present invention, after removing an interfering substance that interferes in measuring salivary sugar using a filter, the saliva from which the interference substance is removed is measured through a biosensor equipped with an enzyme compound, The measurement accuracy can be improved.

1 shows an exemplary diagram for explaining the sample measurement system of the present invention.
FIG. 2 shows the configuration of an embodiment of the collecting device shown in FIG. 1 .
FIG. 3 shows an exemplary view of the biosensor structure shown in FIG. 1 .
FIG. 4 shows an exemplary view of the biosensor electrode structure shown in FIG. 3 .
5 shows an exemplary view for explaining the sample measurement method of the present invention.
6 is a view showing an experimental graph in which the concentration of fine sugar is measured by the sample measurement system of the present invention.

Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described below in detail in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but will be embodied in various different forms, and only these embodiments allow the disclosure of the present invention to be complete, and common knowledge in the art to which the present invention pertains It is provided to fully inform those who have the scope of the invention, and the present invention is only defined by the scope of the claims.

The terminology used herein is for the purpose of describing the embodiments, and is not intended to limit the present invention. In this specification, the singular also includes the plural unless specifically stated otherwise in the phrase. As used herein, "comprises" and/or "comprising" refers to the presence of one or more other components, steps, operations and/or elements mentioned. or addition is not excluded.

Unless otherwise defined, all terms (including technical and scientific terms) used herein may be used with the meaning commonly understood by those of ordinary skill in the art to which the present invention belongs. In addition, terms defined in a commonly used dictionary are not to be interpreted ideally or excessively unless specifically defined explicitly.

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to the accompanying drawings. The same reference numerals are used for the same components in the drawings, and repeated descriptions of the same components are omitted.

In the embodiments of the present invention, a sample, for example, saliva is collected, an interfering material that interferes with the measurement of the sample is removed from the sample, and then glucose contained in the saliva is measured through a biosensor. An object of the present invention is to provide a biosensor capable of improving the measurement accuracy of contained substances and a sample measurement system using the same.

Here, the filter for removing interfering substances removes large molecules and foreign substances present in saliva when the sample is saliva, and substances that interfere with measuring sugars in saliva, such as proteins, amylases and ions can be separated.

In the biosensor, a working electrode, a reference electrode, and at least one recognition electrode for recognizing a sample, which are electrodes for measuring a sample, are disposed, and the working electrode and the reference electrode are disposed in the longitudinal direction of the sample insertion flow path. The projections of the working electrode (hereinafter, referred to as “working projections”) and the two projections of the reference electrode (hereinafter referred to as “reference projections”) are alternated in the portion corresponding to the sample insertion passage. arranged, the area of the working protrusion and the area ratio of the reference protrusions is equal to or greater than 1, and at least one recognition electrode is adjacent to the working electrode or the reference electrode and has a structure in which it is spaced apart from the working electrode and the reference electrode in parallel, and the sample insertion flow path It may have an electrode structure in which at least one protrusion (hereinafter, referred to as “recognition protrusion”) is disposed on a portion corresponding to .

In this case, the electrode structure of the biosensor may further include a strip recognition electrode for detecting a moment when the biosensor is inserted into the measuring device for measurement.

In such a biosensor, an enzyme compound is inserted into a middle plate in which a sample insertion path is formed, and the enzyme compound may include an enzyme, a polymer, and a catalyst.

In this case, the enzyme can selectively react glucose contained in saliva when the sample is saliva, and the polymer is for attaching the enzyme to the electrodes disposed in the biosensor, and the catalyst is It may be for accelerating the reaction between enzymes and glucose.

The present invention will be described with reference to FIGS. 1 to 6 as follows.

1 shows an exemplary view for explaining the sample measurement system of the present invention, and as shown in FIG. 1 , the sample measurement system 100 according to an embodiment of the present invention includes a collection device 110 and a bio It includes a sensor 200 and a measuring device 300 .

As shown in FIG. 2, the collection device 100 includes a sample collection unit 110 including a sample collection swab 111 for collecting a sample, a filter 120 and a compression tube ( 130).

At this time, when the sample is saliva, the sample collection unit 110 may collect saliva through the sample collection swab 111 , and the saliva collected by the sample collection unit 110 is inserted into the compression tube 130 . After the interfering material contained in the saliva is removed through the filter 120 , it may be provided to the outside through the compression tube 130 , that is, to the biosensor 200 .

That is, the recollection apparatus 100 compresses the sample collection unit 110 in the compression tube 130 in the direction of the compression tube 130 after the saliva is collected, and the collected saliva is contained in the saliva through the filter 120. Interfering substances can be removed, large molecules and foreign substances present in saliva can be removed, and substances that interfere with measuring sugar in saliva such as proteins, amylases and ions can be separated.

The filter 120 may be composed of at least one or more layers. Of course, in the filter 120 in the present invention, an additional material may be added to the above-described material in consideration of the degree of removal of the interference material to be removed from the sample, and the thickness of the manufactured layer may vary. In addition, when the filter 120 is composed of a plurality of layers, layers made of different materials may be sequentially stacked to form a filter.

The biosensor 200 has a function of sensing information, for example, glucose, to be measured from the sample from which the interference material has been removed when the sample collected by the collection device 100 is inserted through the collection device 100 . to perform, the biosensor strip 200 is inserted into the measuring device 300, the sample is recognized based on the sample recognition signal received from the measuring device 300, and the sample measurement signal applied separately from the sample recognition signal A response signal to the glucose contained in the sample is provided to the measurement device 300 through the

As shown in FIG. 3 , the biosensor 200 may include an upper plate 230 , a middle plate 220 and a lower plate 210 , and the lower plate 210 has a biosensor electrode as shown in FIG. 4 . structure is formed.

Specifically, a sample insertion passage is formed in the middle plate 220 , and an enzyme compound 221 is inserted into the sample insertion passage.

Here, since the sample insertion flow path is obvious to those skilled in the art, a detailed description thereof will be omitted.

The enzyme compound 221 is an enzyme for selectively reacting glucose contained in the sample, a polymer for attaching the enzyme to electrodes disposed on the lower plate, and the enzyme and glucose It may include a catalyst (catalyst) for accelerating the reaction.

At this time, the catalyst may contain 1% to 10% of at least one of ferrocene, a ferrocene derivative, a quinone, a quinone derivative, hexaamine ruthenium (III) chloride and a ferricyanide (Ferricyanide) If Russian blue is used, the catalyst may contain 0.001% to 5% of Prussian blue), and the enzyme contains at least one of glucose oxidase and glucose dehydrogenase (GDH) in 1 unit. It may contain ~ 30 units, and the polymer may contain 0.01% to 1.0% by weight of at least one of Chitosan, PVP, Nafion, polyethylene glycol, poly vinyl pyrrolidone, poly vinyl alcohol, agarose and trehalose. there is.

The upper plate 230 is formed on the middle plate 220, and the upper plate 230 has an insertion hole for inserting a sample collected through a collection device in a portion corresponding to the sample insertion passage on the insulating substrate and an outlet for discharging air. is formed

On the lower plate 210 , a working electrode 211 and a reference electrode 212 which are electrodes for measuring a sample on the insulating substrate 211 and at least one recognition electrode 213 for recognizing a sample are provided. ), the working electrode 211 and the reference electrode 212 are spaced apart from each other in the longitudinal direction of the sample insertion flow path, and the working protrusion 211a of the working electrode 211 and The two reference protrusions 212a of the reference electrode 212 are alternately arranged, the area ratio of the working protrusion 211 to the area of the reference protrusions 212 is 1 or more, and at least one recognition electrode 213 is the working protrusion 211 . It has a structure in which the working electrode 211 and the reference electrode 212 are disposed adjacent to the electrode 211 or the reference electrode 212 and spaced apart parallel to each other, and at least one recognition protrusion 213a is formed in a portion corresponding to the sample insertion passage. ) has an electrode structure in which it is disposed.

Here, the lower plate 210 may be formed by overlapping a plurality of metal layers having such an electrode structure. For example, the lower plate 210 is made of a first metal layer 210-2 made of copper, a second metal layer 210-3 made of nickel, and gold having a biosensor electrode structure on the insulating substrate 210-1. The formed third metal layers 210 - 4 may overlap each other.

Furthermore, as shown in FIG. 4, the biosensor electrode structure may further include a strip recognition electrode 214 for detecting whether the biosensor strip is inserted in the measuring device at a portion where the biosensor strip is inserted into the measuring device. there is.

In addition, the working electrode 211 and the reference electrode 212 are spaced apart from each other in the longitudinal direction of the sample insertion passage, and the electrode width in the direction of the measuring device 300 is wider than the electrode width in the sample insertion passage. and one working protrusion 211a is disposed between the two reference protrusions 212a and may be configured to be alternately arranged with each other. In this case, since the area of the working protrusion 211a is formed to be at least larger than the area of the two reference protrusions 212a, it is possible to increase the measurement accuracy of the sample. That is, since salivary sugar is detected to be lower than blood sugar, by making the area of the working protrusion 211a wider, when saliva comes into contact, the response signal by the sample measurement signal can be accurately measured, thereby improving the measurement accuracy of salivary sugar.

In addition, while the recognition electrode 213 is disposed between the reference electrode 212 and the working electrode 211 , the recognition protrusion 213a may be formed at the distal end of the sample insertion passage.

The distance between the working protrusion 211a and the reference protrusion 212a, the distance between the reference protrusion 212a and the recognition protrusion 213a, and the width of each protrusion can be determined in consideration of the measurement accuracy of the sample, and the It may be decided by an individual or a business.

The recognition electrode 214 receives a sample recognition signal for recognizing a sample from the measurement device 300 when the biosensor strip is inserted into the measurement device 300 , thereby receiving a response signal to the sample recognition signal in the measurement device 300 . can recognize the sample.

The reference electrode 212 and the working electrode 211 may also receive a sample measurement signal for measuring a sample from the measurement device 300 when the biosensor strip 200 is inserted into the measurement device 300 , and measure the sample. The signal may be received separately from the sample recognition signal, and the point at which the sample measurement signal is received may be the point at which the biosensor strip 200 is inserted into the measuring device 300 , and the sample is recognized by the recognition electrode 213 . It may have been the time Of course, the measuring device 300 may recognize whether the biosensor strip 200 is inserted through the strip recognition electrode 214 provided in the biosensor strip 200 .

The sample measurement signal applied to the reference electrode 212 and the working electrode 211 is a signal corresponding to a predetermined potential difference between the reference electrode 212 and the working electrode 211, and by applying a different preset voltage, the reference electrode and the working electrode A sample measurement signal can be applied to the electrode.

When the biosensor strip 200 is inserted, the measuring device 300 recognizes that the biosensor strip 200 is inserted through the strip recognition electrode 214 provided in the biosensor strip 200, and recognizes the biosensor. By providing the sample recognition signal to the electrode 213, it is determined whether the sample has been in contact with the biosensor, and when it is determined that the sample has been contacted, the sample measurement signal is provided to the reference electrode 212 and the working electrode 212 to measure Receive the response signal of the sample and measure the sample.

Here, the measuring device 300 may measure salivary sugar when the sample is saliva.

In addition, the measuring device 300 may provide a result value for the salivary sugar measured through the display means, or may additionally provide a color for the glucose level.

5 is an exemplary view for explaining the method for measuring a sample of the present invention, and the process for measuring salivary sugar is as follows.

When the sample measurement method is described with reference to FIG. 5, as shown in FIGS. 5A and 5B, after collecting the subject's saliva using the sample collection unit of the collection device, the sample collection unit is inserted into a compression tube equipped with a filter. do

And, as shown in FIGS. 5c and 5d, after inserting the biosensor strip having the biosensor electrode structure of the present invention into the measuring device, the interfering material is removed from the saliva collected by the collecting and collecting unit through compression of the collecting device. It is filtered or removed and inserted into the insertion hole of the biosensor.

At this time, the measuring device provides a sample recognition signal to the recognition electrode of the biosensor by recognizing that the biosensor strip is inserted, and provides a sample measurement signal to the reference electrode and the working electrode when saliva is inserted and recognized through the insertion hole can

When the sample measurement signal is applied to the electrodes of the biosensor, as shown in FIG. 5E , a response signal to the sample measurement signal is received, thereby providing a measurement result for the response signal through the display means.

Here, the measurement result provided through the display means may provide a result of several steps depending on the measurement value, and the numerical range and the result according to the salivary sugar are obvious to those skilled in the art, so the detailed description is omitted.

As described above, the biosensor, the sample measuring system, and the sample measuring method according to the embodiments of the present invention collect a sample, for example, saliva, remove an interfering material that interferes with the sample measurement from the collected sample, and then use the biosensor. By measuring glucose contained in saliva through the

In addition, in the biosensor, sample measurement system, and sample measurement method according to embodiments of the present invention, an enzyme compound is provided in saliva from which the interference material is removed after removing an interfering substance that interferes with measuring salivary sugar using a filter By measuring through an established biosensor, it is possible to improve the measurement accuracy for salivary sugar.

In particular, in the biosensor, the sample measuring system and the sample measuring method according to the embodiments of the present invention, 1 unit to 30 units of at least one of glucose oxidase and glucose dehydrogenase (GDH) is used in the enzyme compound. Enzyme, Chitosan, PVP, Nafion, polyethylene glycol, poly vinyl pyrrolidone, poly vinyl alcohol, a polymer in which at least one of agarose and trehalose is used in wt 0.01% to 1.0%, ferrocene, By including a catalyst in which at least one of ferrocene derivatives, quinones, quinone derivatives, hexaamineruthenium (III) chloride and ferricyanide is used in 1% to 10% (Prussian blue is used) If it is, the catalyst may contain 0.001% to 5% of Prussian blue), and as shown in FIG. 6 , it may be possible to measure the concentration of fine sugar.

The device described above may be implemented as a hardware component, a software component, and/or a combination of the hardware component and the software component. For example, devices and components described in the embodiments may include, for example, a processor, a controller, an arithmetic logic unit (ALU), a digital signal processor, a microcomputer, a field programmable array (FPA), It may be implemented using one or more general purpose or special purpose computers, such as a programmable logic unit (PLU), microprocessor, or any other device capable of executing and responding to instructions. The processing device may execute an operating system (OS) and one or more software applications running on the operating system. A processing device may also access, store, manipulate, process, and generate data in response to execution of the software. For convenience of understanding, although one processing device is sometimes described as being used, one of ordinary skill in the art will recognize that the processing device includes a plurality of processing elements and/or a plurality of types of processing elements. It can be seen that can include For example, the processing device may include a plurality of processors or one processor and one controller. Other processing configurations are also possible, such as parallel processors.

Software may comprise a computer program, code, instructions, or a combination of one or more thereof, which configures a processing device to operate as desired or is independently or collectively processed You can command the device. The software and/or data may be any kind of machine, component, physical device, virtual equipment, computer storage medium or apparatus, to be interpreted by or to provide instructions or data to the processing device. may be embodied in The software may be distributed over networked computer systems and stored or executed in a distributed manner. Software and data may be stored in one or more computer-readable recording media.

The method according to the embodiment may be implemented in the form of program instructions that can be executed through various computer means and recorded in a computer-readable medium. The computer-readable medium may include program instructions, data files, data structures, etc. alone or in combination. The program instructions recorded on the medium may be specially designed and configured for the embodiment, or may be known and available to those skilled in the art of computer software. Examples of the computer-readable recording medium include magnetic media such as hard disks, floppy disks and magnetic tapes, optical media such as CD-ROMs and DVDs, and magnetic such as floppy disks. - includes magneto-optical media, and hardware devices specially configured to store and execute program instructions, such as ROM, RAM, flash memory, and the like. Examples of program instructions include not only machine language codes such as those generated by a compiler, but also high-level language codes that can be executed by a computer using an interpreter or the like.

As described above, although the embodiments have been described with reference to the limited embodiments and drawings, various modifications and variations are possible from the above description by those skilled in the art. For example, the described techniques are performed in an order different from the described method, and/or the described components of the system, structure, apparatus, circuit, etc. are combined or combined in a different form than the described method, or other components Or substituted or substituted by equivalents may achieve an appropriate result.

Therefore, other implementations, other embodiments, and equivalents to the claims are also within the scope of the following claims.

Claims (14)

In the biosensor electrode structure for sample measurement,
A working electrode and a reference electrode, which are electrodes for measuring a sample, are disposed to be spaced apart from each other in the longitudinal direction of the sample insertion passage,
In a portion corresponding to the sample insertion passage, a working protrusion, which is a protrusion of the working electrode, and a reference protrusion, which are two protrusions of the reference electrode, are alternately arranged, and the area of the working protrusion and the area ratio of the reference protrusions is 1 or more,
At least one recognition electrode for recognizing the sample has a structure in which the working electrode or the reference electrode is adjacent to and spaced apart from the working electrode and the reference electrode in parallel,
Wherein the at least one recognition electrode has at least one recognition protrusion disposed on a portion corresponding to the sample insertion passage,
Biosensor electrode structure, characterized in that.
According to claim 1,
The recognition protrusion
The biosensor electrode structure, characterized in that it is disposed adjacent to the distal end of the sample insertion passage.
According to claim 1,
The at least one recognition electrode is
A biosensor structure, characterized in that the sample recognition is performed in response to a sample recognition signal applied independently of the sample measurement signal applied to the working electrode and the reference electrode.
According to claim 1,
Strip recognition electrode for detecting the moment when the biosensor having the electrode structure of the biosensor is inserted into the measuring device for measurement
Biosensor structure, characterized in that it further comprises.
In the biosensor structure for sample measurement,
A working electrode and a reference electrode, which are electrodes for measuring a sample, and at least one recognition electrode for recognizing the sample are disposed on the insulating substrate, and the working electrode and the reference electrode are provided in a sample insertion path are spaced apart from each other in the longitudinal direction of the , and in a portion corresponding to the sample insertion passage, a working protrusion, which is a protrusion of the working electrode, and a reference protrusion, which are two protrusions of the reference electrode, are alternately arranged, and the area of the working protrusion and the reference The area ratio of the protrusions is equal to or greater than 1, and the at least one recognition electrode is adjacent to the working electrode or the reference electrode and has a structure in which the working electrode and the reference electrode are spaced apart from each other in parallel, and corresponding to the sample insertion channel. a lower plate having a structure in which at least one recognition protrusion, which is a protrusion, is disposed on the portion;
a middle plate having the sample insertion passage formed therein, and into which an enzyme compound is inserted; and
A top plate in which an insertion hole for inserting the sample and an outlet for discharging air are formed in a portion corresponding to the sample insertion path on the insulating substrate
A biosensor structure comprising a.
6. The method of claim 5,
The enzyme compound is
When the sample is saliva, an enzyme for selectively reacting glucose contained in the saliva, a polymer for attaching the enzyme to the electrodes disposed on the lower plate and a catalyst for accelerating the reaction between the enzyme and the glucose,
The enzyme is
Contains 1 unit to 30 units of at least one of glucose oxidase and glucose dehydrogenase (GDH),
The polymer is
Chitosan, PVP, Nafion, polyethylene glycol, poly vinyl Pyrrolidone, poly vinyl alcohol, and at least one of agarose (agarose) and trehalose (trehalose) in wt 0.01% to 1.0%,
The catalyst is
A biosensor structure comprising 1% to 10% of at least one of ferrocene, ferrocene derivative, quinone, quinone derivative, hexaamine ruthenium (III) chloride and ferricyanide.
7. The method of claim 6,
The catalyst is
A biosensor structure comprising 0.001% to 5% of the Prussian blue.
6. The method of claim 5,
The at least one recognition electrode is
A biosensor structure, characterized in that the sample recognition is performed in response to a sample recognition signal applied independently of the sample measurement signal applied to the working electrode and the reference electrode.
a sampling device comprising: a sampling unit for collecting a sample;
A working electrode and a reference electrode, which are electrodes for measuring the sample from which the interference material has been removed, and at least one recognition electrode for recognizing the sample from which the interference material has been removed are disposed, the working electrode and the reference electrode are arranged to be spaced apart from each other in the longitudinal direction of the sample insertion flow path, and in a portion corresponding to the sample insertion flow path, a working protrusion, which is a protrusion of the working electrode, and a reference protrusion, which is two protrusions of the reference electrode, are alternately arranged, The ratio of the area of the working protrusion to the area of the reference protrusions is equal to or greater than 1, and the at least one recognition electrode is adjacent to the working electrode or the reference electrode and has a structure in which the working electrode and the reference electrode are spaced apart from each other in parallel. , a biosensor having an electrode structure in which a recognition protrusion, which is at least one protrusion, is disposed on a portion corresponding to the sample insertion passage; and
After the strip of the biosensor is inserted, when the sample from which the interference material has been removed through the collection device is inserted into the biosensor, the interference material is removed based on the sample measurement signal received through the working electrode and the reference electrode. Measuring device that measures the sample
A sample measurement system comprising a.
10. The method of claim 9,
the filter is
When the sample is saliva, large molecules and foreign substances present in the saliva are removed, and substances containing proteins, amylases and ions that interfere with the measurement of sugar in the saliva are separated. A sample measurement system with
10. The method of claim 9,
The biosensor
a lower plate having the electrode structure;
a middle plate having the sample insertion passage formed therein, and into which an enzyme compound is inserted; and
A top plate in which an insertion hole for inserting a sample from which the interference material has been removed and an outlet for discharging air are formed in a portion corresponding to the sample insertion passage
A sample measurement system comprising a.
12. The method of claim 11,
The enzyme compound is
When the sample is saliva, an enzyme for selectively reacting glucose contained in the saliva, a polymer for attaching the enzyme to the electrodes disposed on the lower plate and a catalyst for accelerating the reaction between the enzyme and the glucose,
The enzyme is
Contains 1 unit to 30 units of at least one of glucose oxidase and glucose dehydrogenase (GDH),
The polymer is
Chitosan, PVP, Nafion, polyethylene glycol, poly vinyl Pyrrolidone, poly vinyl alcohol, and at least one of agarose (agarose) and trehalose (trehalose) in wt 0.01% to 1.0%,
The catalyst is
A sample measurement system comprising 1% to 10% of at least one of ferrocene, ferrocene derivative, quinone, quinone derivative, hexaamineruthenium(III) chloride, and ferricyanide.
13. The method of claim 12,
The catalyst is
The sample measurement system, characterized in that it contains 0.001% to 5% of the Prussian blue.
collecting a sample using a collection means and removing an interfering substance that interferes with measuring the sample from the sampled sample using a filter;
contacting the biosensor with the sample from which the interference material has been removed; and
When the contact of the sample from which the interference material has been removed is recognized by at least one recognition electrode of the biosensor, a sample measurement signal is applied to a working electrode and a reference electrode of the biosensor to apply the interference material Measuring the response signal for the removed sample
including,
The working electrode and the reference electrode are disposed to be spaced apart from each other in the longitudinal direction of the sample insertion flow path, and in a portion corresponding to the sample insertion flow path, the working protrusion, which is a protrusion of the working electrode, and the reference protrusion, which are two protrusions of the reference electrode, are alternated. arranged, wherein the ratio of the area of the working protrusion to the area of the reference protrusions is 1 or more, and the at least one recognition electrode is adjacent to the working electrode or the reference electrode and is spaced apart from the working electrode and the reference electrode in parallel A method for measuring a sample, wherein the recognition protrusion, which is at least one protrusion, is disposed in a portion corresponding to the sample insertion passage.

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