KR20220029825A - Slug powder with reduced odor and improved quality and manufacturing method - Google Patents

Slug powder with reduced odor and improved quality and manufacturing method Download PDF

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KR20220029825A
KR20220029825A KR1020200109311A KR20200109311A KR20220029825A KR 20220029825 A KR20220029825 A KR 20220029825A KR 1020200109311 A KR1020200109311 A KR 1020200109311A KR 20200109311 A KR20200109311 A KR 20200109311A KR 20220029825 A KR20220029825 A KR 20220029825A
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slug
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김일두
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01Instant products; Powders; Flakes; Granules
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    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • A23L19/12Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops of potatoes
    • A23L19/15Unshaped dry products, e.g. powders, flakes, granules or agglomerates
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06Enzymes
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    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065Microorganisms
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/40Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
    • A23L3/44Freeze-drying
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00Shaping or working of foodstuffs characterised by the products
    • A23P10/20Agglomerating; Granulating; Tabletting
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
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    • A23V2200/00Function of food ingredients
    • A23V2200/15Flavour affecting agent
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
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    • A23V2250/1638Undefined mineral extract
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    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
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    • A23V2300/00Processes
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    • A23V2300/00Processes
    • A23V2300/31Mechanical treatment
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Abstract

The present invention relates to cicada larva powder with reduced odor and improved quality and a manufacturing method thereof. More specifically, the method comprises: a first step of selecting, washing, and pulverizing cicada larvae as raw materials; a second step of pre-processing cicada larva powder; a third step of mixing a fermented pu'er tea extraction by-product and deep-sea water with the preprocessed cicada larva powder to firstly ferment the mixture; a fourth step of firstly aging the fermented product; a fifth step of mixing the aged product and preprocessed fermented pine needles to secondly ferment the mixture; a sixth step of aging the mixture; a seventh step of sterilizing the product; an eighth step of drying the sterilized product; and a ninth step of packaging and commercializing the product.

Description

냄새 저감과 품질이 향상된 굼벵이 분말 및 그 제조방법{Slug powder with reduced odor and improved quality and manufacturing method}Slug powder with reduced odor and improved quality and manufacturing method

본 발명은 냄새 저감과 품질이 향상된 굼벵이 분말 및 그 제조방법에 관한 것으로, 보다 상세하게는 제 1단계는 원료(굼벵이) 선별, 세척 및 분말화 단계, 제 2단계는 원료의 전(前)처리 단계, 제 3단계는 전(前)처리된 원료에 보이차 추출 부산물 발효물 처리 단계, 제 4단계는 1차 숙성 단계, 제 5단계는 전(前)처리된 발효 솔잎 처리 단계, 제 6단계는 2차 숙성 단계, 제 7단계는 살균 단계, 제 8단계는 건조 단계, 제 9단계는 포장 및 제품 단계를 포함한 냄새 저감과 품질이 향상된 굼벵이제조 방법에 관한 것이다.The present invention relates to slug powder with improved odor reduction and quality and a method for manufacturing the same. Step, Step 3 is a step of processing the pre-treated raw material with Puer tea extraction by-product fermented product, Step 4 is the first ripening step, Step 5 is the pre-treated fermentation pine needle treatment step, Step 6 relates to a method for producing slugs with improved odor reduction and quality, including the secondary aging step, the 7th step sterilization step, the 8th step drying step, and the 9th step packaging and product steps.

굼벵이는 한방에서는 약재로 사용하는 것으로 초가집의 썩은 이엉 속이나 흙 속, 각종 식물의 뿌리 근처에 살며 식품학적으로는 고(高)단백 식품으로 머리는 단단한 반구 모양이며, 피부는 얇으며 몸은 연하고 우윳빛을 띠고 있고 그 효과는 간암 , 간경화, 심장병, 성인병, 파상풍, 악성 종기 및 치매 치료에 있다고 알려져 있다. Slugs are used for medicinal purposes in oriental medicine. They live in the rotting thatch of thatch houses, in the soil, and near the roots of various plants. It has a milky color and its effect is known to be in the treatment of liver cancer, cirrhosis, heart disease, geriatric diseases, tetanus, malignant boils and dementia.

감잎은 식품학적으로 비타민 C와 P, 칼슘 및 탄닌이 풍부하다고 알려져 있다. 이로써 가지고 있는 효과는 피부 및 주름 개선, 노화 방지, 항산화 작용, 혈액 순환 촉진, 감기예방, 고혈압이나 당뇨병 등 각종 성인병 예방 및 치료, 숙취 해소, 여성 생리통 해소 등이 있다고 알려져 있다. Persimmon leaves are known to be rich in vitamins C and P, calcium, and tannins from food sources. It is known to have effects such as skin and wrinkle improvement, anti-aging, antioxidant action, blood circulation promotion, cold prevention, prevention and treatment of various adult diseases such as high blood pressure and diabetes, relieving hangover, and relieving menstrual pain in women.

보이차는 이미 그 기능성은 알려져 있어 함유된 성분으로는 테아닌, 가바(GABA), 플라보노이드, 폴리페놀, 카테킨, 아미노산, 켐페롤, 케르세틴, 밀세틴, 플라보놀 글리코시 등이며 약리적인 효능은 고(古)의학서에 의하면 콜레스테롤 감소, 비만 억제, 면역력 증강, 숙취 제거, 동맥 경화 예방과 치료, 혈압 상승 억제 등으로 알려져 있다. Pu'er's tea is already known for its functionality, so the ingredients contained include theanine, GABA, flavonoids, polyphenols, catechins, amino acids, kaempferol, quercetin, milcetin, flavonol glycosy, etc. ) According to the medical literature, it is known for reducing cholesterol, suppressing obesity, enhancing immunity, removing hangover, preventing and treating arteriosclerosis, and suppressing increase in blood pressure.

솔잎은 우리나라의 전통적 향신료이며, 한국, 일본 및 중국 등지에 분포되어 종류로는 주로 왜송, 조선송(적송과 흑송)으로 사용부위는 주로 잎으로 그 효능으로는 당뇨병 예방, 신경계 질환, 순환계 질환, 위장 질환 및 간장 질환 등이 있다고 알려져 있다. 용도로써는 차(tea), 주스, 송편 및 가공 식품으로 제조 된다. Pine needles are a traditional spice in Korea, and are distributed in Korea, Japan, and China. The types are mainly Japanese pine and Korean pine (red pine and black pine), and the parts used are mainly leaves. It is known that there are gastrointestinal diseases and liver diseases. For use, it is manufactured into tea, juice, songpyeon and processed foods.

종래의 굼벵이 이용하여 접목시킨 기술로는 등록번호 1016166880000호 “굼벵이를 이용한 기능성 식품 제조 방법”등록 번호 1019383480000호 “굼벵이로부터 지방산 오일 및 수용성 펩타이드를 분리하는 방법” 등록 번호 1019530970000호 “홍삼을 먹은 굼벵이를 이용한 기능성 식품 제조 방법” 등록 번호 101579410000호 “굼벵이 분변물을 이용한 비료 및 이의 제조성 방법”등록 번호 1018885490000호 “굼벵이를 함유하는 다이어트 음료 및 그 제조방법”등이 있고, 감잎을 이용한 특허로는 등록 번호 2018759580000호 “감잎 및 감 종자를 이용한 기능성 차 제조방법”등록 번호 1013819500000호 “감잎 발효차 및 이의 제조방법”등록 번호 1016181210000호 “가을 감잎을 이용한 감잎 장아찌 제조방법”등록 번호 1011207130000호 “천연 섬유의 감잎 염색방법”등록 번호 1013732630000호 “면역기능 증진 활성이 있는 감잎 유래 다당 분획물 및 이의 제조방법”등록 번호 1017455970000호 “발효 감잎, 발효 감잎 추출물 및 이의 용도”등이 있고, 한편, 보이차를 이용한 특허로써는 출원 번호 諺”“금귤 과피를 이용한 보이차” 출원 번호 諺0048727” “보이차 추출물을 유효 성분으로 포함하는 노화로 인한 인지 기능 장애 예방 또는 치료용 약제학적 조성물 ”출원 번호 諺0113863”“보이차 추출물을 함유하는 마스크팩용 바이오셀룰로오스 시트 및 이의 제조 방법”등록 번호 諺”“보이차 함유한 돼지 족발 제조 방법 및 이를 이용하여 제조된 돼지 족발”등록 번호 諺”“보이차고 제조방법”등록 번호 諺”“송이버섯 향과 영양성분이 가미된 송이 보이차와 이의 제조 방법 ”등록 번호 諺”“산양삼과 보이차를 포함하는 차 조성물 및 그의 제조 방법” 등이 있다. As a technique grafted using conventional slugs, registration number 1016166880000 “method of manufacturing functional food using slugs” registration number 1019383480000 “method of separating fatty acid oil and water-soluble peptide from slugs” registration number 1019530970000 “slugs eating red ginseng” Functional food manufacturing method using” Registration No. 101579410000 “Fertilizer using slug feces and manufacturing method thereof” Registration No. 1018885490000 “Diet drink containing slugs and manufacturing method”, etc., registered as a patent using persimmon leaves No. 2018759580000 “Method for manufacturing functional tea using persimmon leaves and persimmon seeds” Registration No. 1013819500000 “Fermented persimmon leaf tea and manufacturing method thereof” Registration No. 1016181210000 “Method for manufacturing pickled persimmon leaves using autumn persimmon leaves” Registration No. 1011207130000 “Natural fiber Persimmon leaf dyeing method” Registration No. 1013732630000 “Polysaccharide fraction derived from persimmon leaf with immune function enhancing activity and manufacturing method thereof” Registration No. 1017455970000 “Fermented persimmon leaf, fermented persimmon leaf extract and its use”, etc. For example, Application No. 諺” “Puer tea using kumquat peel” Application No. 諺0048727” “Pharmaceutical composition for the prevention or treatment of cognitive impairment due to aging that contains Pueri tea extract as an active ingredient” Application No. 諺0113863” Bio-cellulose sheet for mask pack containing extract and manufacturing method thereof”Registration No. There are “Peace pine tea with pine mushroom flavor and nutritional ingredients added to it and its manufacturing method,” “Registration No.

또한, 솔잎을 이용한 특허로는 등록 번호 1019669830000호 “솔잎추출물을 함유하는 대사 증후군 개선용 조성물”등록 번호 101243530000호 “솔잎 추출액을 포함한 모발의 생장 촉진용 샴푸 조성물 및 그 방법” 등록 번호 1013749930000호 “솔잎의 송진 성분 제거 방법”등록 번호 1009529020000호 “숙성 된 솔잎 성분이 함유된 화장비누 조성물 및 그 제조방법”등과 같이 출원 및 등록되었지만 이들은 본 특허와 같이 감잎, 감미숙과, 보이차 추출된 부산물 발효 처리물, 발효 솔잎을 이용한 첨단 가공 기술을 접목한 냄새 저감과 품질이 향상된 굼벵이 분말 생산방법에 관한 기술과 그 사용이 지금까지 보고 된 바가 없었다. In addition, as a patent using pine needles, registration number 1019669830000 “composition for improving metabolic syndrome containing pine needle extract” registration number 101243530000 “shampoo composition for promoting hair growth including pine needle extract and method therefor” registration number 1013749930000 “pine needles” The method of removing the rosin component of “Registration No. 1009529020000” was applied for and registered as “Cosmetic soap composition containing aged pine needles and its manufacturing method”, but these were fermented by-products extracted from persimmon leaves, unripe fruits, and Pu’er tea as in this patent. There has been no report on the technology and use of slug powder production method with improved odor reduction and quality by combining cutting-edge processing technology using water and fermented pine needles.

굼벵이를 소비자 기호도 향상의 목적과 산업화를 위해서 곤충류 자체에 함유한 냄새의 저감과 품질이 향상된 최종 제품의 제조가 요구되는 실정이다. For the purpose of improving consumer preference and industrialization of slugs, it is required to reduce the odor contained in insects and to manufacture final products with improved quality.

따라서 냄새 저감과 품질이 향상된 굼벵이 분말 제조 시 소비자에게 안전성이 인정되는 전통 식품 재료와 이를 이용한 발효 및 친환경 가공 기술의 접목으로 냄새 저감과 품질이 향상된 굼벵이 분말 제조 방법을 개발하는데 그 목적이 있다.Therefore, the purpose of this is to develop a method for producing slug powder with improved odor reduction and improved quality by combining traditional food ingredients that are safe to consumers when manufacturing slug powder with improved odor and quality, and fermentation and eco-friendly processing technology using the same.

이에 본 발명은, 제 1단계는 원료인 굼벵이를 선별, 세척, 분말화하는 단계, 제 2단계는 구비된 페트리디쉬에 감부산물 혼합분말을 넣고 상기 감부산물 혼합분말 위에 필터 페이퍼를 깐 다음, 상기 필터 페이퍼 위에 굼벵이분말을 넣은 후, 감자 플레이크와 증류수가 1~3 : 97~99로 혼합된 감자 플레이크 함유액으로 2분간 스프레이 분사하고, 상기 페트리디쉬를 밀폐시킨 후 항온기로 30℃에서 12~24시간을 유지하는 굼벵이분말을 전처리하는 단계, 제 3단계는 상기 전(前)처리된 굼벵이분말 : 보이차 추출 부산 발효물 : 해양 심층수를 혼합 하여 1차 발효하는 단계, 제 4단계는 발효물을 1차 숙성하는 단계, 제 5단계는 1차 숙성 처리된 숙성물 : 생수 : 전(前)처리된 발효 솔잎을 혼합하여 2차 발효하는 단계, 제 6단계는 2차 발효된 발효물을 2차 숙성하는 단계, 제 7단계는 2차 숙성된 숙성물을 살균 처리하는 단계, 제 8단계는 건조하는 단계, 제 9단계는 포장 및 제품화하는 단계를 포함하는 것을 기술적 특징으로 한다. Accordingly, in the present invention, the first step is a step of selecting, washing, and pulverizing the raw material slugs, and the second step is putting a mixed powder of persimmon by-product in a petri dish provided with a filter paper on the mixed powder of persimmon by-product, and then After putting the slug powder on the filter paper, spray the potato flakes with a mixture of potato flakes and distilled water in a ratio of 1-3: 97-99 for 2 minutes, seal the Petri dish, and then use a thermostat at 30°C for 12 to 24 The step of pre-treating the slug powder that maintains time, the third step is the pre-treated slug powder: Puer tea extract by-product fermented product: the step of primary fermentation by mixing deep sea water, the fourth step is the fermentation of the fermented product The first ripening step, the fifth step is the first aging-treated aged product: bottled water: the second fermentation step by mixing the pre-treated fermented pine needles, the sixth step is the second fermentation of the secondly fermented product The aging step, the 7th step is a step of sterilizing the second-aged aged product, the 8th step is a drying step, and the 9th step is a step of packaging and commercialization.

상기 분말화하는 단계는, 원료인 굼벵이를 선별 후, 마이크로 버블기로 10분간 세척한 다음, 건조기로 60℃에서 8시간 건조하고, 건조된 굼벵이를 진공 믹서기로 10분 분쇄하여 50메쉬 크기로 분말화하는 것을 기술적 특징으로 한다. In the powdering step, the raw material slugs are selected, washed with a micro bubbler for 10 minutes, dried at 60° C. with a dryer for 8 hours, and the dried slugs are pulverized with a vacuum mixer for 10 minutes to powder into a 50 mesh size. is a technical feature.

상기 감부산물 혼합분말은, 감미숙과 : 감잎 : 게르마늄 함유수가 3 : 2 : 5 (w/w/w)로 진공믹서기에 넣어 10분간 혼합한 후, 혼합된 혼합물을 살균된 가아제 3겹을 통과한 추출물에 유산균 0.2 중량%, 펙틴 효소(pectinase) 0.01 중량%을 각각 첨가한 다음, 온기에서 38℃, 8~10시간 건조시킨 후 동결 건조하고, 동결건조된 것을 50메쉬로 분말화하는 것을 기술적 특징으로 한다. The mixed powder of persimmon by-products was mixed with unripe fruit: persimmon leaf: germanium-containing water 3: 2: 5 (w/w/w) in a vacuum mixer for 10 minutes, and then the mixed mixture was passed through three layers of sterilized gauze. After adding 0.2% by weight of lactic acid bacteria and 0.01% by weight of pectinase to the extract, respectively, drying at 38°C in warm temperature for 8 to 10 hours, freeze-drying, and powdering the freeze-dried product into 50 mesh do it with

상기 제1차 발효하는 단계는, 상기 전(前)처리된 굼벵이분말 : 보이차 추출 부산 발효물 : 해양 심층수를 5 : 2~4.5 : 0.5~3로 혼합한 후, 25℃에서 24~72시간, 4℃에서 48시간 숙성하는 것을 기술적 특징으로 한다. The first fermentation step is, after mixing the pre-treated slug powder: Puer tea extract by-product: deep sea water at 5: 2 to 4.5: 0.5 to 3, and then at 25° C. for 24 to 72 hours. , It is technically characterized by aging at 4°C for 48 hours.

상기 부산 발효물은, 보이차에서 추출된 부산물 300g을 열풍 건조기로 60℃, 8~10시간 건조 후, 5분간 분말화하여 부산물 분말을 만들고, 탁주 술지게미 500g을 살균된 가아제 2겹을 통과 후 거른 액을 원심 분리기로 3000rpm,10분간 분리하여 발효용 탁주 술지게미를 만든 후, 부산물 분말 10 중량%, 발효용 탁주 술지게미 30 중량%, 멸균수 60 중량%을 혼합한 혼합물을 항온기로 25±2℃에서 24~48시간 발효하는 것을 기술적 특징으로 한다. The fermented by-product is a by-product of 300 g extracted from Pu'er tea, dried with a hot air dryer at 60° C. for 8 to 10 hours, then pulverized for 5 minutes to make a by-product powder, and 500 g of sake lees is passed through two layers of sterilized gauze and filtered. was separated with a centrifuge at 3000rpm for 10 minutes to make takju sake lees for fermentation, and then a mixture of 10% by weight of by-product powder, 30% by weight of takju sake lees for fermentation, and 60% by weight of sterilized water was heated in a thermostat at 25±2℃ 24 It is technically characterized as fermenting for ~48 hours.

상기 2차 발효하는 단계는, 1차 숙성 처리된 숙성물 : 생수 : 전(前)처리된 발효 솔잎을 25~35 : 50~65 : 0~25 (w/w/w)로 혼합한 후, 항온기로 25℃에서 24~72시간, 4℃에서 48시간 숙성 처리하는 것을 기술적 특징으로 한다. In the second fermentation step, the first-aged aged product: mineral water: pre-treated fermented pine needles are mixed at 25-35: 50-65: 0-25 (w/w/w), It is technically characterized by aging at 25°C for 24 to 72 hours and at 4°C for 48 hours with a thermostat.

상기 발효솔잎은, 솔잎 1kg을 수돗물에 3회 세척 및 2~3시간 동안 물기를 제거한 후, 1.0~1.5㎝ 절단하여 스텐통에 해양 심층수 5리터를 함께 혼합하여, 100℃에서 10분 끓여서 1차 표면의 왁스(wax)를 제거 하고, 2차 잔류 왁스(wax)를 제거와 탄닌(tannin)추출을 위해 습식 초음파기기로 28 KHz로 30분 처리한 다음, 항온기로 60℃에서 8~10시간 건조 한 후, 떫은 감즙을 2분간 분무하여 30±2℃에서 24시간 발효하는 것을 기술적 특징으로 한다.The fermented pine needles are, after washing 1 kg of pine needles 3 times in tap water and removing water for 2 to 3 hours, cut 1.0 to 1.5 cm, mix 5 liters of deep sea water in a stainless steel container, boil at 100 ° C for 10 minutes, and boil first To remove the wax on the surface, and to remove the secondary residual wax and extract tannins, it is treated with a wet ultrasonic device at 28 KHz for 30 minutes, and then dried at 60℃ with a thermostat for 8-10 hours. Then, the astringent persimmon juice is sprayed for 2 minutes and fermented at 30±2℃ for 24 hours.

본 발명의 냄새 저감과 품질이 향상된 굼벵이 분말 및 그 제조방법에 의하면, 굼벵이를 이용하여 냄새 저감과 품질이 향상된 굼벵이 분말을 제조 시 소비자 기호도 향상을 위해서 친환경 가공 기술을 접목하여 냄새가 저감 되고 품질이 향상된 최종 고(高) 품질의 굼벵이 분말을 제조함으로써, 새로운 식품 자원으로써의 고(高)부가가치화와 대외 경쟁력을 제공할 수 있는 발명이라 하겠다. According to the slug powder with improved odor reduction and quality of the present invention and its manufacturing method, eco-friendly processing technology is applied to improve consumer preference when slug powder is used to reduce odor and improve quality by using slugs to reduce odor and improve quality. It is an invention that can provide high added value and external competitiveness as a new food resource by manufacturing improved final high quality slug powder.

도 1은 본 발명의 바람직한 실시 예를 나타내는 공정도1 is a process diagram showing a preferred embodiment of the present invention;

본 발명을 첨부된 도면을 참조하여 상세히 설명하면 다음과 같다. The present invention will be described in detail with reference to the accompanying drawings as follows.

이하, 도 1은 본 발명의 냄새 저감과 품질이 향상된 굼벵이 분말 및 그 제조 공정도로서, 본 발명에서는 원료인 굼벵이 선별, 세척, 분말화하는 단계, 상기 굼벵이 분말의 전(前)처리 단계, 전(前)처리된 원료에 보이차 추출 부산 발효물로 1차 발효 처리하는 단계, 1차 숙성 단계, 전(前)처리된 발효솔잎 2차 발효처리하는 단계, 2차 숙성 단계, 살균 단계, 건조 단계, 포장 및 제품화 단계를 포함하며 그의 제조방법을 설명하면 다음과 같다. Hereinafter, FIG. 1 is a slug powder with improved odor reduction and quality of the present invention and a manufacturing process diagram thereof. In the present invention, the raw material slug selection, washing, and powdering steps, the pre-treatment step of the slug powder, the pre- ( Pre) primary fermentation treatment of processed raw materials with fermented by-product of Puer tea extraction, primary aging stage, secondary fermentation of pre-treated fermented pine needles, secondary aging stage, sterilization stage, drying stage , packaging and commercialization steps, and the manufacturing method thereof will be described as follows.

(1) 원료인 굼벵이 선별, 세척, 분말화하는 단계 (1) Steps of screening, washing, and pulverizing raw material slugs

원료인 굼방에를 대구 약령시에서 구입하여 육안으로 보아서 이상이 없는 것을 선별 후 마이크로 버블기(삼원기기, K-25, 한국)로 10분간 세척한 다음, 건조기(대원, W-132, 한국)를 사용하여 60℃에서 8시간 건조 후 진공 믹서기(삼일, WA-234, 한국)로 10분 분쇄하여 체눈 크기-0.05mm, 선의 굵기-0.56mm로 형성된 체(sieve)를 통과 시켜 50메쉬 크기로 분말화 하여 사용하였다. The raw material, Gumbangae, was purchased from Yangnyeong-si, Daegu, and the ones that were not visually abnormal were washed for 10 minutes with a microbubble machine (Samwon Machinery, K-25, Korea), and then dried with a dryer (Daewon, W-132, Korea). After drying at 60℃ for 8 hours, pulverize for 10 minutes with a vacuum mixer (Samil, WA-234, Korea) and pass through a sieve formed with a sieve size -0.05mm and wire thickness -0.56mm to form a powder with a size of 50 mesh was used.

이때, 마이크로 버블은 미세한 기포로써 초미립자 형태로 물속에서 생성되어 기포 직경이 10~50㎛로써 수면으로 일반 기포보다 상승 속도가 느려서 용존 산소의 증가로 악취 제거, 어류 성장 촉진 및 최종 살균 ,탈취효과, 피부병 개선 등이 있는 것으로 알려져 있다. At this time, microbubbles are created in water in the form of ultra-fine particles as fine bubbles, and the bubble diameter is 10-50㎛, and the rate of rise to the water surface is slower than that of general bubbles. It is known to improve skin diseases.

(2) 굼벵이분말의 전(前)처리 단계 (2) Pre-treatment step of slug powder

페트리디쉬(지름×높이= 8.5㎝×㎝)에 0.3~0.4㎝높이로 감부산물(감미숙과 + 감잎) 혼합분말을 골고루 펼쳐 넣고 위에 필터 페이퍼(WhatmanTM No.1) 1장을 깔고 다시 위에 (1)에서 처리된 분말화된 굼벵이분말을 0.5㎝높이로 첨가 후 골고루 펼친 다음, 감자 플레이크(식물성 접착제) : 증류수가 1~3 : 97~99 (w/w)로 혼합된 감자 플레이크 함유액을 2분간 스프레이 분사한 후, 뚜껑을 밀폐시켜 항온기(삼일,WR-129, 한국)에서 30℃, 12~24시간을 유지하여 전 처리하였다.Spread the mixed powder of persimmon by-products (unripe persimmon + persimmon leaves) evenly in a Petri dish (diameter × height = 8.5 cm × cm) to a height of 0.3 to 0.4 cm. After adding the powdered slug powder treated in (1) to a height of 0.5 cm and spreading it evenly, potato flakes (vegetable adhesive): Potato flakes containing distilled water 1-3: 97-99 (w/w) After spraying for 2 minutes, the lid was sealed and pre-treated at 30° C. in a thermostat (Samil, WR-129, Korea) for 12 to 24 hours.

이때, 상기 감부산물(감미숙과+감잎) 혼합분말은, 감미숙과(평균 무게 5~10g) : 감잎(성잎 : 왁스 제거됨 : 100℃에서 1시간 열처리함) : 게르마늄 함유수(시중 구입)를 3 : 2 : 5 (w/w/w)를 진공 믹서기(삼일, WA-234, 한국)에 넣어 10분 혼합 후, 그 혼합물을 살균된 가아제 3겹을 통과시킨 추출물에, 유산균(디에이치 팜, 한국) 0.2 중량%과 펙틴 효소(pectinase) 0.01 중량%을 각각 첨가한 다음, 항온기(삼일,WR-129, 한국)로 38℃, 8~10시간 살균처리 후 이를 동결 건조한 후 50 메쉬로 분말화하였다. At this time, the mixed powder of the persimmon by-product (unripe sweet fruit + persimmon leaves) is: unripe sweet fruit (average weight 5 to 10 g): persimmon leaves (adult leaves: wax removed: heat-treated at 100°C for 1 hour): germanium-containing water (commercially purchased) 3: 2: 5 (w/w/w) was put in a vacuum mixer (Samil, WA-234, Korea) and mixed for 10 minutes, and then the mixture was passed through three layers of sterilized gauze to the extract, lactic acid bacteria (DH palm) , Korea) 0.2% by weight and 0.01% by weight of pectinase are added, respectively, and then sterilized at 38°C for 8-10 hours with a thermostat (Samil, WR-129, Korea), freeze-dried and powdered to 50 mesh got angry

(3) 1차 발효하는 단계 (3) Primary fermentation step

(2)의 전(前)처리된 굼벵이분말 : 보이차 추출 부산 발효물 : 해양 심층수(강원도 속초 생산, 디프스, 한국)를 5 : 2~4.5 : 0.5~3를 혼합하여 20리터 스텐통에 넣은 후 25℃에서 24~72시간, 4℃에서 48시간 숙성 처리하였다. (2) pre-treated slug powder: Puer tea extraction by-product: Deep sea water (produced in Sokcho, Gangwon-do, Deeps, Korea) 5: 2 to 4.5: 0.5 to 3 mixed in a 20 liter stainless steel container After adding, aging was performed at 25°C for 24-72 hours and at 4°C for 48 hours.

이때, 보이차 추출 부산물 발효물은, 보이차 추출된 부산물 300g을 열풍 건조기(대한, K-25, 한국)를 이용하여 60℃, 8~10시간 건조 후 진공 건조기(삼일, DN-32,한국)에서 5분간 분말화(①-보이차 추출된 부산물 분말)하였다. At this time, the fermented by-product of Pu-erh tea extraction is made by drying 300 g of the by-product extracted from Pu-erh tea using a hot air dryer (Daehan, K-25, Korea) at 60° C. for 8 to 10 hours, followed by a vacuum dryer (Samil, DN-32, Korea). ) was powdered for 5 minutes (①-puer tea extracted by-product powder).

탁주 술 지게미 500g을 살균된 가아제 2겹을 통과 후 거른 액을 원심 분리기(비전 과학, VS-4000, 한국)으로 3000rpm, 10분간 분리 후 상등액을 발효용 탁주 술지게미(②)로 하여 사용하고, 희석 액으로는 멸균수(③)로 사용하여, 보이차 추출된 부산물 분말 10 중량%, 발효용 탁주 술 지게미 30 중량%, 멸균수 60 중량%을 혼합하여 항온기(삼일,kj-12, 한국)로 25±2℃에서 24~48시간 발효하였다. After passing 500 g of Takju sake lees through 2 layers of sterilized gauze, the filtered solution is separated by a centrifugal separator (Vision Science, VS-4000, Korea) at 3000rpm for 10 minutes, and the supernatant is used as Takju sake lees for fermentation (②) and diluted As sterilized water (③), 10% by weight of powdered by-product powder extracted from Puerai tea, 30% by weight of fermented takju sake lees, and 60% by weight of sterilized water were mixed and heated to a thermostat (Samil, kj-12, Korea). Fermentation was carried out at ±2°C for 24-48 hours.

이러한 1차 발효하는 단계를 거침으로써 굼벵이분말의 냄새를 저감시킬 수가 있다. It is possible to reduce the smell of slug powder by going through this primary fermentation step.

(4) 1차 숙성 단계 (4) 1st ripening stage

(3)의 발효된 발효물을 4~5℃에서 8~12시간 숙성 하였다. The fermented product of (3) was aged at 4-5°C for 8-12 hours.

(5) 2차 발효하는 단계 (5) secondary fermentation step

(4)의 1차 숙성 처리된 숙성물 : 생수 : 전(前)처리된 발효솔잎을 25~35 : 50~65 : 0~25 (w/w/w)로 혼합하여 5리터 스텐통에 넣고, 항온기(삼일,kj-12, 한국)를 이용하여 25℃에서 24~72시간, 4℃에서 48시간 숙성 처리하였다. Aged product treated with primary aging in (4): Bottled water: Mix pre-treated fermented pine needles at 25-35: 50-65: 0-25 (w/w/w) and put it in a 5-liter stainless steel container , using a thermostat (Samil, kj-12, Korea) was aged for 24 to 72 hours at 25 °C and 48 hours at 4 °C.

이때, 상기 숙성물과 발효솔잎은 다시백(크린ŽN :생활연구소)에 넣어 사용하였다. At this time, the aged product and fermented pine needles were put in a dashi bag (Clean ŽN: Life Research Institute) and used.

한편, 발효솔잎은, 솔잎(경북 봉화산) 1kg을 수돗물에 3회 세척 후 2~3시간 솥쿠리에 담아 물기를 제거한 다음, 1.0~1.5㎝ 정도로 잘라서, 10리터 스텐통에 해양 심층수 5리터를 함께 혼합하여 100℃에서 10분 끓여 1차 표면의 왁스(wax)를 제거하고, 2차 잔류 왁스(wax)를 제거와 탄닌(tannin)추출을 위해, 습식 초음파기기(스텐 통 : 가로×세로×높이=28㎝*?*㎝后㎝, 테콘사, 미국)로 28 KHz로 30분 처리한 다음, 항온기(삼일,kj-12, 한국)를 이용하여 60℃에서 8~10시간 건조 후 떫은 감즙을 2분간 분무하여 30±2℃에서 24시간 발효하였다. On the other hand, for fermented pine needles, wash 1 kg of pine needles (Mt. Bonghwa, Gyeongsangbuk-do) in tap water three times, put them in a pot for 2-3 hours to remove moisture, cut them to 1.0-1.5 cm, and mix with 5 liters of deep sea water in a 10-liter stencil. and boil at 100℃ for 10 minutes to remove the wax on the primary surface, and to remove the secondary residual wax and extract tannins, a wet ultrasonic device (stenn barrel: width × length × height = 28cm*?*cm后cm, Tecon Inc., USA) at 28 KHz for 30 minutes, and then dried at 60℃ for 8-10 hours using a thermostat (Samil, kj-12, Korea), and then astringent persimmon juice 2 It was fermented for 24 hours at 30±2℃ by spraying for minutes.

상기 2차 발효하는 단계를 1차 발효와 더불어 냄새를 더욱 저감시킬 수가 있다. In the second fermentation step, it is possible to further reduce the smell along with the first fermentation.

(6) 2차 숙성 단계 (6) Second ripening stage

(5)의 처리된 발효물을 4~5℃에서 8~12시간 숙성 하였다. The fermented product treated in (5) was aged at 4-5° C. for 8-12 hours.

(7) 살균 단계 (7) Sterilization step

(6)의 숙성된 내용물을 고압 증기 살균기(삼원, K-25, 한국)를 이용하여 121℃, 1.5기압, 15분간 처리하여 살균하였다. The aged contents of (6) were sterilized by treatment at 121°C, 1.5 atmospheres, and 15 minutes using a high-pressure steam sterilizer (Samwon, K-25, Korea).

(8) 건조 단계 (8) drying step

(7)의 살균된 내용물을 60~70℃에서 8~12시간 열풍 건조하였다. The sterilized contents of (7) were dried with hot air at 60-70° C. for 8-12 hours.

(9) 포장 및 제품화 단계 (9) Packaging and commercialization stage

본 단계는 (9)을 포장 및 제품화 단계이다. This step (9) is the packaging and commercialization step.

이하, 도면을 참고하여 본 발명의 바람직한 실시 예는 다음과 같다. Hereinafter, preferred embodiments of the present invention with reference to the drawings are as follows.

(1) 전(前)처리된 굼벵이분말에 보이차 추출 부산 발효물의 혼합 비율에 의한 굼벵이 분말의 관능검사 실시 예 (1) Example of sensory test of slug powder by mixing ratio of fermented by-product of Puer tea extraction to pre-treated slug powder

본 발명은 전(前)처리된 굼벵이분말에 보이차 추출 부산 발효물의 혼합 비율에 의한 굼벵이 분말의 관능검사 실시 측정은 표1과 같이 나타났다. In the present invention, the sensory test measurement of slug powder by the mixing ratio of the by-product fermented Puer tea extraction to the pre-treated slug powder is shown in Table 1.

전(前)처리된 굼벵이분말에 보이차 추출 부산 발효물의 혼합 비율 에 의한 굼벵이 분말의 관능검사 Sensory test of slug powder by mixing ratio of fermented by-product of Puerto tea extraction to pre-treated slug powder 물(생수) : 전(前)처리된 굼벵이분말
: 보이차 추출 부산 발효물 (w/w/w)
Water (mineral water): pre-treated slug powder
: Pu'er tea extraction by-product (w/w/w)
색상colour taste incense 종합적인
기호도
comprehensive
symbol
5 : 2 : 35:2:3 3.23.2 3.23.2 3.23.2 3.13.1 5 : 3 : 2 5:3:2 3.13.1 3.23.2 3.43.4 3.63.6 5 : 4 : 15: 4: 1 3.63.6 4.64.6 4.34.3 4.84.8 5 : 4.5 : 0.5 5:4.5:0.5 3.13.1 3.13.1 3.33.3 3.43.4

1) 주어진 값은 실험 3회 평균값, 관능검사요원(10명)에 의한 5점법(아주 나쁨:1점, 나쁨:2점, 보통:3점, 좋음:4점, 아주 좋음:5점)1) The given value is the average value of three experiments, and a 5-point method by sensory inspectors (10 people) (Very poor: 1 point, Bad: 2 points, Normal: 3 points, Good: 4 points, Very good: 5 points)

① 관능 평가 ① Sensory evaluation

표 1을 참고하면 색상, 맛, 향, 종합적인 기호도 평가는 30~60세 성인 남녀 10명의 패널 요원에게 5점 강도법(효과 없음 :1점, 효과 조금 있음 :2점, 보통임 :3점, 조금 좋음 :4점, 매우 좋음 :5점)으로 평점하여 처리하였다. Referring to Table 1, the color, taste, aroma, and overall preference evaluation were conducted using a 5-point intensity method (no effect: 1 point, slightly effective: 2 points, moderate: 3 points) for 10 male and female adult males and females aged 30 to 60 years. , a little good: 4 points, very good: 5 points).

② 전(前)처리된 원료에 보이차 추출 부산 발효물의 혼합 비율에 의한 굼벵이 분말의 관능검사 결과 ② Sensory test result of slug powder according to the mixing ratio of the by-product fermented Puer tea extraction to the pre-treated raw material

표 1을 참고하면 전(前)처리된 굼벵이분말에 보이차 추출 부산 발효물의 혼합 비율에 의한 굼벵이 분말의 관능검사 결과 색상, 맛, 향, 종합적인 기호도에서 물(생수) : 전(前)처리된 굼벵이분말 : 보이차 추출 부산 발효물(w/w/w)의 혼합 비율은 5 : 4 : 1(w/w/w) 처리구가 가장 좋았다. Referring to Table 1, as a result of sensory examination of slug powder by the mixing ratio of the by-product fermented Puer tea extraction to the pre-treated slug powder, water (mineral water): pre-treatment The mixing ratio of dried slug powder: Puer tea extract by-product (w/w/w) was the best in the 5: 4: 1 (w/w/w) treatment group.

(2) 전前)처리된 굼벵이분말에 보이차 추출 부산 발효물의 혼합 비율에 의한 굼벵이분말의 당도, pH 및 산도 측정 실시 예 (2) Example of measurement of sugar content, pH and acidity of slug powder by mixing ratio of by-product fermented by-product of Puer tea extraction to treated slug powder

본 발명은 전前)처리된 원료에 보이차 추출 부산 발효물의 혼합 비율에 의한 굼벵이 분말의 당도, pH 및 산도 측정 은 표2와 같이 나타났다. In the present invention, the measurement of sugar content, pH and acidity of slug powder by the mixing ratio of the by-product fermented Puer tea extraction to the pre-treated raw material is shown in Table 2.

전前)처리된 굼벵이분말에 보이차 추출 부산 발효물 혼합 비율에 의한 굼벵이 분말의 당도, pH 및 산도Sugar content, pH and acidity of slug powder according to the mixing ratio of the by-product fermented product of Puerto tea extraction to the pre-treated slug powder 물(생수) : 전(前)처리된 굼벵이분말
: 보이차 추출 부산 발효물 (w/w/w)
Water (mineral water): pre-treated slug powder
: Pu'er tea extraction by-product (w/w/w)
당도
(Brix)
sugar content
( Brix)
pH pH 산도(%) Acidity (%)
5 : 2 : 35:2:3 4.014.01 5.875.87 1.031.03 5 : 3 : 2 5:3:2 3.493.49 5.895.89 1.021.02 5 : 4 : 15: 4: 1 3.443.44 5.885.88 1.041.04 5 : 4.5 : 0.5 5:4.5:0.5 3.433.43 5.915.91 1.031.03

1) 주어진 값은 실험 3회 평균값.1) The given value is the average value of three experiments.

① 당도, pH 와 산도 측정 ① Measurement of sugar content, pH and acidity

표 2를 참고하면 당도는 시료 500 ㎕(마이크로 리터)를 취하여 당도계(Hand refractometer, kruss, 독일)을 사용하여 당도(브릭스 , Brix)를 측정 하였다. pH와 산도 측정은 굼벵이 분말을 증류수로 5배 희석 후 pH는 pH 미터(Metrohm Ltd., 스위스)로 측정하였고 , 산도 측정은 굼벵이 분말 희석액 10ml를 취하여 pH가 8.3에 도달할 때까지 0.1N NaOH 용액으로 적정하여 이때 소비된 NaOH 용액의 양을 젖산(%)양으로 환산하였다.Referring to Table 2, for sugar content, 500 μl (microliter) of a sample was taken and sugar content (Brix , Brix) was measured using a saccharometer (Hand refractometer, kruss, Germany). The pH and acidity were measured with a pH meter (Metrohm Ltd., Switzerland) after diluting the slug powder 5 times with distilled water. For the pH and acidity measurement, 10 ml of the slug powder diluted solution was taken and 0.1N NaOH solution until the pH reached 8.3. was titrated to convert the amount of NaOH solution consumed at this time into the amount of lactic acid (%).

적정 산도(%) = 0.1N-NaOH 소모량 ×0.1N-NaOH 팩트 ×0.09 / 시료 중량(g) ×100 Titration acidity (%) = 0.1N-NaOH consumption ×0.1N-NaOH fact ×0.09 / sample weight (g) ×100

② 전前)처리된 원료에 보이차 추출 부산 발효물 혼합 비율에 의한 굼벵이 분말의 당도, pH 및 산도 측정 결과 ② Measurement result of sugar content, pH and acidity of slug powder by mixing ratio of by-product fermented product extracted from Puerhu tea to pre)treated raw materials

표 2에서 나타나듯이 pH와 산도(%) 는 모든 처리구에서 큰 차이가 나타나지 아니하였고, 당도는 물(생수) : 전(前)처리된 굼벵이분말 : 보이차 추출 부산 발효물(w/w/w) 의 혼합 비율은 5 : 2 : 3(w/w/w) 처리구가 가장 높은 값을 나타냈다. As shown in Table 2, there was no significant difference in pH and acidity (%) in all treatment groups, and sugar content was water (mineral water): pre-treated slug powder: Puerhu tea extract by-product (w/w/w) ) of the mixing ratio of 5: 2: 3 (w/w/w) treatment showed the highest value.

(3) 전(前)처리된 굼벵이분말에 보이차 추출 부산 발효물 혼합 비율에 의한 굼벵이 분말의 항산화 효과 실시 예 (3) Example of antioxidant effect of slug powder by mixing ratio of by-product fermented by-product of Puer tea extraction to pre-treated slug powder

본 발명은 전(前)처리된 굼벵이분말에 보이차 추출 부산물 발효물의 혼합 비율에 의한 굼벵이 분말의 항산화 효과는 표 3과 같이 나타났다. In the present invention, the antioxidant effect of the slug powder by the mixing ratio of the fermented Puerto tea extract by-product to the pre-treated slug powder is shown in Table 3.

전(前)처리된 원료에 보이차 추출 부산 발효물 혼합 비율에 의한 굼벵이 분말의 항산화 효과Antioxidant effect of slug powder by the mixing ratio of Puer tea extraction by-product ferment to pre-treated raw materials 물(생수) : 전(前)처리된 굼벵이분말
: 보이차 추출 부산 발효물 (w/w/w)
Water (mineral water): pre-treated slug powder
: Pu'er tea extraction by-product (w/w/w)
DPPH
(%Inhibition)
DPPH
(%Inhibition)
총 폴리페놀
(㎍/g)
total polyphenols
(μg/g)
5 : 2 : 35:2:3 70.5170.51 20.9120.91 5 : 3 : 2 5:3:2 65.3365.33 17.3217.32 5 : 4 : 15: 4: 1 55.2055.20 16.3416.34 5 : 4.5 : 0.5 5:4.5:0.5 50.2950.29 15.2115.21

1) 주어진 값은 실험 3회 평균값.1) The given value is the average value of three experiments.

① 항산화 효과 측정 ① Measurement of antioxidant effect

DPPH(2,2-diphenyl-1-picryl-hydrazyl-hydrate) 라디컬 소거능은 브로이스의 방법으로 DPPH 용액은 0.1%의 농도로 사용하였고 다음과 같이 측정하였다. 엘리자(ELISA) 마이크로리더기를 사용하여 흡광도를 측정하였다. DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity was measured as follows. Absorbance was measured using an ELISA microreader.

또한, 총 페놀함량은 Folin-Ciocalteu법을 이용하여 측정하였다. 측정기기로는 엘리자(ELISA) 마이크로리더기(Infinite F50,Tecan, Switzerland)를 사용하였다. In addition, the total phenol content was measured using the Folin-Ciocalteu method. As a measuring device, an ELISA microreader (Infinite F50, Tecan, Switzerland) was used.

② 전(前)처리된 굼벵이분말에 보이차 추출 부산 발효물의 혼합 비율에 의한 굼벵이 분말의 항산화 효과 측정 결과 ② Measurement result of antioxidant effect of slug powder by mixing ratio of fermented by-product of Puer tea extraction to pre-treated slug powder

표 2를 참고하면 전(前)처리된 굼벵이분말에 보이차 추출 부산 발효물 혼합 비율에 의한 굼벵이 분말의 항산화 효과는 디피피에이치(DPPH)와 총 폴리페놀 함량은 물(생수) : 전(前)처리된 굼벵이분말 : 보이차 추출 부산 발효물(w/w/w) 의 혼합 비율은 5 : 2 : 3 (w/w/w) 처리구가 가장 높은 값을 나타냈다. Referring to Table 2, the antioxidant effect of the slug powder by the mixing ratio of the by-product fermented extract extracted from Pu'er to the pre-treated slug powder is DPPH, and the total polyphenol content is water (mineral water): before. The mixing ratio of the treated slug powder: Puer tea extract by-product (w/w/w) showed the highest value in the 5: 2: 3 (w/w/w) treatment group.

(4) 전(前)처리된 발효솔잎 첨가량에 따른 굼벵이 분말의 관능검사 실시 예 (4) Example of sensory test of slug powder according to the amount of pre-treated fermented pine needles added

본 발명은 전(前)처리된 발효 솔잎 첨가량에 따른 굼벵이 분말의 관능검사 측정은 표 4와 같이 나타났다. In the present invention, the sensory test measurements of slug powder according to the amount of pre-treated fermented pine needles are shown in Table 4.

전(前)처리된 발효솔잎 첨가량에 따른 굼벵이 분말의 관능검사Sensory test of slug powder according to the amount of pre-treated fermented pine needles added 생수: 1차 숙성 처리된 숙성물
: 전(前)처리된 발효 솔잎 (w/w/w)
Bottled water: Aged water that has undergone primary aging
: Pre-treated fermented pine needles (w/w/w)
색상colour taste incense 종합적인
기호도
comprehensive
symbol
65 : 35 : 065:35:0 3.23.2 3.33.3 3.43.4 3.23.2 60 : 30 : 1060:30:10 3.83.8 4.74.7 4.34.3 4.74.7 55 : 30 : 1555:30:15 3.63.6 4.34.3 4.14.1 4.04.0 50 : 25 : 2550:25:25 3.23.2 3.43.4 3.43.4 3.53.5

1) 주어진 값은 3회 평균값, 관능검사요원(10)명에 의한 5점법(아주 나쁨:1점, 나쁨:2점, 보통:3점, 좋음:4점, 아주 좋음:5점)1) The given value is the average of 3 times, and a 5-point method by 10 sensory inspectors (Very poor: 1 point, Bad: 2 points, Normal: 3 points, Good: 4 points, Very good: 5 points)

① 관능 평가 ① Sensory evaluation

표 4를 참고하면 색상, 맛 향, 종합적인 기호도 평가는 30~60세 성인 남녀 10명의 패널 요원에게 5점 강도법(효과 없음 :1점, 효과 조금 있음 :2점, 보통임 :3점, 조금 좋음 :4점, 매우 좋음 :5점)으로 평점하여 처리하였다. Referring to Table 4, the color, taste, and overall preference evaluation was conducted using a 5-point intensity method (no effect: 1 point, slightly effective: 2 points, moderate: 3 points, Somewhat good: 4 points, very good: 5 points).

② 전(前)처리된 발효솔잎 첨가량에 따른 굼벵이분말의 관능검사 측정 결과 표 4를 참고하면 발효 솔잎 첨가량에 따른 굼벵이 분말의 관능검사 결과 색상, 맛, 향, 종합적인 기호도에서 물(생수) : 1차 숙성된 숙성물 : 발효 솔잎 =60% : 30% : 10% 처리구가 가장 좋았다. ② Sensory test results of slug powder according to the amount of pre-treated fermented pine needles Refer to Table 4, the sensory test results of slugs powder according to the amount of added fermented pine needles Water (mineral water): The first aged material: Fermented pine needles = 60%: 30%: 10% The treatment group was the best.

(5) 전(前)처리된 발효 솔잎 첨가량에 따른 굼벵이 분말의 당도, pH 및 산도 측정 실시 예 (5) Example of measurement of sugar content, pH and acidity of slug powder according to the amount of pre-treated fermented pine needles added

본 발명은 전(前)처리된 발효 솔잎 첨가량에 따른 굼벵이 분말의 당도, pH 및 산도 측정은 표 5와 같이 나타났다. In the present invention, the measurement of sugar content, pH and acidity of slug powder according to the amount of pre-treated fermented pine needles added is shown in Table 5.

전(前)처리된 발효 솔잎 첨가량에 따른 굼벵이 분말의 당도, pH 및 산도 Sugar content, pH and acidity of slug powder according to the amount of pre-treated fermented pine needles added 생수 : 1차 숙성된 숙성물
: 전(前)처리된 발효 솔잎 (w/w/w)
Bottled water: 1st aged water
: Pre-treated fermented pine needles (w/w/w)
당도
(Brix)
sugar content
( Brix)
pH pH 산도(%) Acidity (%)
65 : 35 : 065:35:0 3.403.40 5.335.33 1.211.21 60 : 30 : 1060:30:10 3.433.43 5.435.43 1.221.22 55 : 30 : 1555:30:15 3.333.33 5.545.54 1.191.19 50 : 25 : 2550:25:25 3.013.01 5.445.44 1.181.18

① 당도, pH 와 산도 측정 ① Measurement of sugar content, pH and acidity

표 5를 참고하면 당도는 시료 500 ㎕(마이크로 리터)를 취하여 당도계(Hand refractometer, kruss, 독일)을 사용하여 당도(브릭스 , Brix)를 측정 하였다. pH와 산도 측정은 굼벵이 분말을 증류수로 5배 희석 후 pH는 pH 미터(Metrohm Ltd., 스위스)로 측정하였고 , 산도 측정은 굼벵이 분말 희석액 10ml를 취하여 pH가 8.3에 도달할 때까지 0.1N NaOH 용액으로 적정하여 이때 소비된 NaOH 용액의 양을 젖산(%)양으로 환산하였다.Referring to Table 5, the sugar content (Brix, Brix) was measured using a saccharometer (Hand refractometer, kruss, Germany) after taking 500 μl (microliter) of the sample. The pH and acidity were measured with a pH meter (Metrohm Ltd., Switzerland) after diluting the slug powder 5 times with distilled water. For the pH and acidity measurement, 10 ml of the slug powder diluted solution was taken and 0.1N NaOH solution until the pH reached 8.3. was titrated to convert the amount of NaOH solution consumed at this time into the amount of lactic acid (%).

적정 산도(%) = 0.1N-NaOH 소모량 ×0.1N-NaOH 팩트 ×0.09 / 시료 중량(g) ×100 Titration acidity (%) = 0.1N-NaOH consumption ×0.1N-NaOH fact ×0.09 / sample weight (g) ×100

② 전(前)처리된 발효 솔잎 첨가량에 따른 굼벵이 분말의 당도, pH 및 산도 측정 결과 ② Measurement result of sugar content, pH and acidity of slug powder according to the amount of pre-treated fermented pine needles added

표 5에서 나타나듯이 pH(5.33~5.54)와 산도(1.18~1.22%)는 모든 처리구에서 큰 차이가 나타나지 아니하였고, 당도는 생수 : 전(前)처리 원료 분말 : 전(前)처리된 발효 솔잎(w/w/w)의 혼합 비율은 60 : 30 : 10(w/w/w) 처리구가 가장 높은 값을 나타냈다. As shown in Table 5, there was no significant difference in pH (5.33~5.54) and acidity (1.18~1.22%) in all treatment groups, and sugar content in mineral water: pre-treated raw material powder: pre-treated fermented pine needles The mixing ratio of (w/w/w) was 60: 30: 10 (w/w/w) treatment group showed the highest value.

(6) 전(前)처리된 발효 솔잎 첨가량에 따른 굼벵이 분말의 항산화 효과 실시 예 (6) Example of antioxidant effect of slug powder according to the amount of pre-treated fermented pine needles added

본 발명은 전(前)처리된 발효 솔잎 첨가량에 따른 굼벵이 분말의 항산화 효과는 표 6과 같이 나타났다. In the present invention, the antioxidant effect of slug powder according to the amount of pre-treated fermented pine needles added is shown in Table 6.

전(前)처리된 발효 솔잎 첨가량에 따른 굼벵이 분말의 항산화 효과Antioxidant effect of slug powder according to the amount of pre-treated fermented pine needles added 생수 : 1차 숙성된 숙성물 :
전(前)처리된 발효 솔잎 (w/w/w)
Bottled water: 1st aged water:
Pre-treated fermented pine needles (w/w/w)
DPPH
(%Inhibition)
DPPH
(%Inhibition)
총 폴리페놀
(㎍/g)
total polyphenols
(μg/g)
65 : 35 : 065:35:0 50.5050.50 10.9210.92 60 : 30 : 1060:30:10 55.3155.31 13.3813.38 55 : 30 : 1555:30:15 64.2164.21 15.3115.31 50 : 25 : 2550:25:25 60.5560.55 13.0113.01

1) 주어진 값은 실험 3회 평균값.1) The given value is the average value of three experiments.

① 항산화 효과 측정 ① Measurement of antioxidant effect

DPPH(2,2-diphenyl-1-picryl-hydrazyl-hydrate) 라디컬 소거능은 브로이스의 방법으로 DPPH 용액은 0.1%의 농도로 사용하였고 다음과 같이 측정하였다. 엘리자(ELISA) 마이크로리더기를 사용하여 흡광도를 측정하였다. DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity was measured as follows. Absorbance was measured using an ELISA microreader.

또한, 총 페놀함량은 Folin-Ciocalteu법을 이용하여 측정하였다. 측정기기로는 엘리자(ELISA) 마이크로리더기(Infinite F50,Tecan, Switzerland)를 사용하였다. In addition, the total phenol content was measured using the Folin-Ciocalteu method. As a measuring device, an ELISA microreader (Infinite F50, Tecan, Switzerland) was used.

② 전(前)처리된 발효 솔잎 첨가량에 따른 굼벵이 분말의 항산화 효과 측정 결과 표 6을 참고하면 발효 솔잎 첨가량에 따른 굼벵이 분말의 항산화 효과는 디피피에이치(DPPH)와 총 폴리페놀 함량은 생수 : 전(前)처리 원료 분말 : 전(前)처리된 발효 솔잎 (w/w/w)의 혼합 비율은 55 : 30 : 15 (w/w/w) 처리구가 가장 높은 값을 나타냈다. ② As a result of measuring the antioxidant effect of slug powder according to the amount of pre-treated fermented pine needles, refer to Table 6, the antioxidant effect of slug powder according to the amount of added fermented pine needles is DPPH, and the total polyphenol content is mineral water: The mixing ratio of pre-treated raw material powder: pre-treated fermented pine needles (w/w/w) showed the highest value in 55:30:15 (w/w/w) treatment.

Claims (8)

제 1단계는 원료인 굼벵이를 선별, 세척, 분말화하는 단계,
제 2단계는 구비된 페트리디쉬에 감부산물 혼합분말을 넣고 상기 감부산물 혼합분말 위에 필터 페이퍼를 깐 다음, 상기 필터 페이퍼 위에 굼벵이분말을 넣은 후, 감자 플레이크와 증류수가 1~3 : 97~99로 혼합된 감자 플레이크 함유액으로 2분간 스프레이 분사하고, 상기 페트리디쉬를 밀폐시킨 후 항온기로 30℃에서 12~24시간을 유지하는 굼벵이분말을 전처리하는 단계,
제 3단계는 상기 전(前)처리된 굼벵이분말 : 보이차 추출 부산 발효물 : 해양 심층수를 혼합 하여 1차 발효하는 단계,
제 4단계는 발효물을 1차 숙성하는 단계,
제 5단계는 1차 숙성 처리된 숙성물 : 생수 : 전(前)처리된 발효 솔잎을 혼합하여 2차 발효하는 단계,
제 6단계는 2차 발효된 발효물을 2차 숙성하는 단계,
제 7단계는 2차 숙성된 숙성물을 살균 처리하는 단계,
제 8단계는 건조하는 단계,
제 9단계는 포장 및 제품화하는 단계를 포함하는 것을 특징으로 하는 냄새 저감과 품질이 향상된 굼벵이 분말 제조 방법.
The first step is to select, wash, and powder the raw material slugs,
In the second step, put the mixed powder of persimmon by-product in the provided petri dish, put filter paper on the mixed powder of persimmon by-product, put slug powder on the filter paper, and then put potato flakes and distilled water in a ratio of 1-3: 97-99. Spraying the mixed potato flake-containing solution for 2 minutes, sealing the Petri dish, and then pre-treating the slug powder that is maintained at 30° C. for 12 to 24 hours with a thermostat;
The third step is a step of primary fermentation by mixing the pre-treated slug powder: Puer tea extract by-product: deep sea water;
The fourth step is a step of first aging the fermented product,
Step 5 is a step of secondary fermentation by mixing the first-aged aged product: mineral water: pre-treated fermented pine needles;
The sixth step is a step of second aging the second fermented fermented product,
The seventh step is a step of sterilizing the second-aged aged product,
The eighth step is a step of drying,
The ninth step is a method for producing slug powder with improved odor reduction and quality, characterized in that it includes a step of packaging and commercialization.
제 1항에 있어서,
상기 분말화하는 단계는,
원료인 굼벵이를 선별 후, 마이크로 버블기로 10분간 세척한 다음, 건조기로 60℃에서 8시간 건조하고, 건조된 굼벵이를 진공 믹서기로 10분 분쇄하여 50메쉬 크기로 분말화하는 것을 특징으로 하는 냄새 저감과 품질이 향상된 굼벵이 분말 제조 방법.
The method of claim 1,
The powdering step is
After screening the raw slugs, washed with a microbubble machine for 10 minutes, dried at 60° C. for 8 hours with a dryer, and pulverized for 10 minutes with a vacuum mixer to reduce odor, characterized in that the dried slugs are pulverized to a size of 50 mesh. and improved quality slug powder manufacturing method.
제 1항에 있어서,
상기 감부산물 혼합분말은,
감미숙과 : 감잎 : 게르마늄 함유수가 3 : 2 : 5 (w/w/w)로 진공믹서기에 넣어 10분간 혼합한 후, 혼합된 혼합물을 살균된 가아제 3겹을 통과한 추출물에 유산균 0.2 중량%, 펙틴 효소(pectinase) 0.01 중량%을 각각 첨가한 다음, 온기에서 38℃, 8~10시간 건조시킨 후 동결 건조하고, 동결건조된 것을 50메쉬로 분말화하는 것을 특징으로 하는 냄새 저감과 품질이 향상된 굼벵이 분말 제조 방법.
The method of claim 1,
The persimmon by-product mixed powder is,
Unripe sweet fruit: Persimmon leaf: Germanium-containing water 3: 2: 5 (w/w/w) put in a vacuum mixer and mixed for 10 minutes, then, the mixed mixture was passed through 3 layers of sterilized gauze and 0.2 wt% of lactic acid bacteria, Odor reduction and quality improved, characterized in that 0.01 wt% of pectinase is added, respectively, and then freeze-dried at 38°C for 8 to 10 hours at warm temperature, and the freeze-dried product is powdered into 50 mesh Method for making slug powder.
제 1항에 있어서,
상기 제1차 발효하는 단계는,
상기 전(前)처리된 굼벵이분말 : 보이차 추출 부산 발효물 : 해양 심층수를 5 : 2~4.5 : 0.5~3로 혼합한 후, 25℃에서 24~72시간, 4℃에서 48시간 숙성하는 것을 특징으로 하는 냄새 저감과 품질이 향상된 굼벵이 분말 제조방법.
The method of claim 1,
The first fermentation step is,
The pre-treated slug powder: Puer tea extraction by-product: Mixing deep sea water in a ratio of 5: 2 to 4.5: 0.5 to 3, followed by aging at 25 ° C for 24 to 72 hours and at 4 ° C for 48 hours A method for producing slug powder with improved odor and improved quality.
제 4항에 있어서,
상기 부산 발효물은,
보이차에서 추출된 부산물 300g을 열풍 건조기로 60℃, 8~10시간 건조 후, 5분간 분말화하여 부산물 분말을 만들고,
탁주 술지게미 500g을 살균된 가아제 2겹을 통과 후 거른 액을 원심 분리기로 3000rpm,10분간 분리하여 발효용 탁주 술지게미를 만든 후,
부산물 분말 10 중량%, 발효용 탁주 술지게미 30 중량%, 멸균수 60 중량%을 혼합한 혼합물을 항온기로 25±2℃에서 24~48시간 발효하는 것을 특징으로 하는 냄새 저감과 품질이 향상된 굼벵이 분말 제조 방법.
5. The method of claim 4,
The by-product fermented product,
300 g of by-products extracted from Pu'er tea are dried with a hot air dryer at 60°C for 8 to 10 hours, and then pulverized for 5 minutes to make by-product powder,
After passing 500 g of Takju sake lees through 2 layers of sterilized gauze, the filtered liquid was separated by a centrifugal separator at 3000 rpm for 10 minutes to make Takju sake lees for fermentation.
Manufacture of slug powder with improved odor and quality, characterized by fermenting a mixture of 10% by weight of by-product powder, 30% by weight of sake lees for fermentation, and 60% by weight of sterilized water at 25±2℃ in a thermostat for 24-48 hours method.
제 1항에 있어서,
상기 2차 발효하는 단계는,
1차 숙성 처리된 숙성물 : 생수 : 전(前)처리된 발효 솔잎을 25~35 : 50~65 : 0~25 (w/w/w)로 혼합한 후, 항온기로 25℃에서 24~72시간, 4℃에서 48시간 숙성 처리하는 것을 특징으로 하는 냄새 저감과 품질이 향상된 굼벵이 분말 제조 방법.
The method of claim 1,
The second fermentation step is
Aged product with primary aging treatment: Bottled water: After mixing pre-treated fermented pine needles at 25~35: 50~65: 0~25 (w/w/w), in a thermostat at 25°C, 24-72 A method for producing slug powder with improved odor reduction and quality, characterized in that it is aged for 48 hours at 4°C.
제 6항에 있어서,
상기 발효솔잎은,
솔잎 1kg을 수돗물에 3회 세척 및 2~3시간 동안 물기를 제거한 후,
1.0~1.5㎝ 절단하여 스텐통에 해양 심층수 5리터를 함께 혼합하여, 100℃에서 10분 끓여서 1차 표면의 왁스(wax)를 제거 하고,
2차 잔류 왁스(wax)를 제거와 탄닌(tannin)추출을 위해 습식 초음파기기로 28 KHz로 30분 처리한 다음, 항온기로 60℃에서 8~10시간 건조 한 후, 떫은 감즙을 2분간 분무하여 30±2℃에서 24시간 발효하는 것을 특징으로 하는 냄새 저감과 품질이 향상된 굼벵이 분말 제조 방법.
7. The method of claim 6,
The fermented pine needles,
After washing 1 kg of pine needles in tap water 3 times and removing water for 2-3 hours,
Cut 1.0~1.5cm, mix 5 liters of deep sea water in a stainless steel container, boil at 100℃ for 10 minutes to remove wax on the primary surface,
In order to remove the secondary residual wax and extract tannins, it is treated with a wet ultrasonic device at 28 KHz for 30 minutes, then dried at 60°C for 8 to 10 hours with a thermostat, and then sprayed with astringent persimmon juice for 2 minutes. A method for producing slug powder with improved odor and improved quality, characterized by fermenting at 30±2℃ for 24 hours.
제 1항 내지 제 7항 중 어느 한 항의 제조방법으로 제조되는 것을 특징으로 하는 냄새 저감과 품질이 향상된 굼벵이 분말.
A slug powder with improved odor and improved quality, characterized in that it is manufactured by the manufacturing method according to any one of claims 1 to 7.
KR1020200109311A 2020-08-28 2020-08-28 Slug powder with reduced odor and improved quality and manufacturing method KR102490386B1 (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101935915B1 (en) * 2017-10-26 2019-01-07 박달수 Food containing cricket and snail mixture and method of manufacture
KR20200048282A (en) * 2018-10-29 2020-05-08 채은희 How to make seasoned meat containing omija extract
KR20200081550A (en) * 2018-12-27 2020-07-08 김덕지 Composition containing an fermented extract of protaetia brevitarsis seulensis as an active ingredient and manufacturing the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101935915B1 (en) * 2017-10-26 2019-01-07 박달수 Food containing cricket and snail mixture and method of manufacture
KR20200048282A (en) * 2018-10-29 2020-05-08 채은희 How to make seasoned meat containing omija extract
KR20200081550A (en) * 2018-12-27 2020-07-08 김덕지 Composition containing an fermented extract of protaetia brevitarsis seulensis as an active ingredient and manufacturing the same

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