KR20220021111A - Apparatus for concentrating biological material - Google Patents

Abstract

The present invention discloses a disposable biomaterial concentrating device that is light and easy to handle, and can recover only a second module in which a biomaterial is concentrated. The biomaterial concentrating device according to the present invention comprises: a first module having an opening part at an upper part and a porous membrane for filtering a sample; a second module that includes a first passage disposed in a vertical direction with respect to the bottom surface of the first module, a second passage disposed on a side surface of the first passage, and a membrane disposed at the inside of an area with which side surfaces of the first passage and second passage come in contact; and a third module disposed in a lower part of the second passage and including a vacuum pump for applying a negative pressure to the first module and the second module, wherein the biomaterial contained in the sample is concentrated in the membrane.

Description

Biomaterial Concentration Device {APPARATUS FOR CONCENTRATING BIOLOGICAL MATERIAL}

The present invention relates to a disposable biomaterial concentration device.

Modern medicine aims to realize the extension of healthy lifespan.

For this purpose, early detection, early treatment, tracking and prevention of diseases are very important means.

For early detection, early treatment, and tracking of diseases, a method for detecting and analyzing biological materials such as bacteria, viruses, antibodies, proteins, and nano-endoplasmic reticulum has been used.

In the stage of preparing a biological material to detect a trace amount of a biological material, a concentration operation of separating and collecting the target material should be preceded.

In general, centrifugation has been used for concentration, but in the case of a biomaterial made of a thin cell membrane, cells are easily broken, and expensive equipment is required.

Recently, a technique using a nano-sized device for protein analysis has been used.

However, in this case, there is a problem in that it is difficult to manufacture the device and it is relatively expensive, so it is difficult to popularize the device. In addition, there are disadvantages in that a high-sensitivity sensor is required for a protein analysis device or accurate analysis is difficult with a small amount of sample.

On the other hand, there is a method of improving detection accuracy by concentrating a biological material even in a small amount of sample using glass or a heavy and expensive concentrator.

However, glass or heavy and expensive concentrators have disadvantages in that the concentrator is large and heavy, and waste treatment is inefficient.

Therefore, there is a need for research on a concentrating device that efficiently concentrates biomaterials using a small-volume and light-weight concentrating device and efficiently treats waste.

It is an object of the present invention to provide a disposable biomaterial concentration device that is light and easy to handle.

Another object of the present invention is to provide an apparatus for concentrating biomaterials that can be easily disposed of without a phenomenon in which contaminants accumulated in a third module are discharged to the outside or flow backward in an upward direction.

Another object of the present invention is to provide a biomaterial concentrating device capable of preventing contamination of a membrane in which biomaterials are concentrated from the outside.

The objects of the present invention are not limited to the above-mentioned objects, and other objects and advantages of the present invention not mentioned can be understood by the following description, and will be more clearly understood by the examples of the present invention. It will also be readily apparent that the objects and advantages of the present invention may be realized by the means and combinations thereof indicated in the appended claims.

The biomaterial concentrating device according to the present invention includes: a first module having an upper opening and closing opening, a porous membrane for filtering a sample sample; A first passage disposed in a vertical direction with respect to the bottom surface of the first module, a second passage disposed on a side surface of the first passage, and an area in which the side surface of the first passage and the side surface of the second passage contact a second module comprising a membrane; and a third module disposed under the second passage and including a vacuum pump for applying negative pressure to the first module and the second module, wherein the biological material contained in the sample sample is concentrated in the membrane.

The biomaterial concentrating device according to the present invention includes: a first module having an upper opening and closing opening, a porous membrane for filtering a sample sample; A first passage disposed in a vertical direction with respect to the bottom surface of the first module, a second passage disposed on a side surface of the first passage, and an area in which the side surface of the first passage and the side surface of the second passage contact a second module comprising a membrane; and a third module disposed on a side surface of the second passage, disposed in a vertical direction with respect to the side surface of the second passage, and including a vacuum pump for applying a negative pressure to the first module and the second module; The biomaterial contained in the sample sample is concentrated in the membrane.

The biomaterial concentrating device according to the present invention is light and easy to handle, and has the effect of enabling diagnosis and diagnosis by directly using the membrane in which the biomaterial is concentrated, or recovering only the second module or membrane having the membrane.

In addition, the biomaterial concentrating device according to the present invention has the effect of easy disposal without the phenomenon that the accumulated contaminants are discharged to the outside or flow backward in the upward direction.

In addition, the biomaterial concentration device according to the present invention can prevent the membrane in which the biomaterial is concentrated from being contaminated from the outside.

In addition to the above-described effects, the specific effects of the present invention will be described together while describing specific details for carrying out the invention below.

1 is a cross-sectional view of a first module 100 according to the present invention.
2 is an exploded cross-sectional view of the second module 200 .
3 is a plan view of the first passage 22 in the second module 200 of the present invention.
4 is a plan view of the second passage 24 in the second module 200 of the present invention.
5 and 6 are diagrams showing examples of shapes of portions to be fused by ultrasonic waves in the first passage 22 and the second passage 24 .
7 is a cross-sectional view of the membrane 28 and the support 30 according to the present invention.
8 and 9 are cross-sectional views of the second module 200 according to the opening/closing control of the valve 32 of the present invention.
10 is a cross-sectional view showing a contracted state (OFF) of the vacuum pump 40 of the present invention.
11 is a cross-sectional view showing an expanded state (ON) of the vacuum pump 40 of the present invention.
12 is a cross-sectional view showing an open state of the locking projection 44 of the present invention.
13 is a cross-sectional view showing a state in which the locking projection 44 of the present invention is closed.
14 is an operation view when the valve 32 and the vacuum pump 40 are OFF in the biomaterial concentrating device of the present invention.
15 is an operation view when the valve 32 is OFF and the vacuum pump 40 is ON in the biomaterial concentrating device of the present invention.
16 is an operation view when the valve 32 and the vacuum pump 40 are ON in the biomaterial concentrating device of the present invention.
17 is a cross-sectional view of an example in which the third module 300 is disposed in a vertical direction with respect to the second module 200 in the biomaterial concentrating apparatus of the present invention.

The above-described objects, features and advantages will be described below in detail with reference to the accompanying drawings, and accordingly, those of ordinary skill in the art to which the present invention pertains will be able to easily implement the technical idea of the present invention. In describing the present invention, if it is determined that a detailed description of a known technology related to the present invention may unnecessarily obscure the gist of the present invention, the detailed description will be omitted. Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings. In the drawings, the same reference numerals are used to indicate the same or similar components.

In the following, that an arbitrary component is disposed on the "upper (or lower)" of a component or "above (or below)" of a component means that any component is disposed in contact with the upper surface (or lower surface) of the component. Furthermore, it may mean that other components may be interposed between the component and any component disposed on (or under) the component.

Also, when it is described that a component is "connected", "coupled" or "connected" to another component, the components may be directly connected or connected to each other, but other components are "interposed" between each component. It should be understood that “or, each component may be “connected,” “coupled,” or “connected,” through another component.

Hereinafter, an apparatus for concentrating a biological material according to some embodiments of the present invention will be described.

In the present invention, in the configuration in which the first module, the second module, and the third module are combined, the biomaterial is concentrated in the membrane located in the second module, and the contaminants accumulated in the third module are disposed of without backflow or external leakage. An object of the present invention is to provide an easy disposable biomaterial concentration device.

The biomaterial concentration apparatus according to the present invention includes a first module 100 , a second module 200 , and a third module 300 .

1 is a cross-sectional view of a first module 100 according to the present invention.

The first module 100 has a predetermined space and height for inputting and filtering a sample sample. And the first module 100 includes a connection passage 12 to be coupled to the second module (200). The connection passage 12 serves to guide the flow of the filtered sample sample to move downward.

A porous membrane 14 for filtering a sample sample is disposed in the first module 100 .

The porous membrane 14 is disposed to remove foreign substances or unnecessary components included in the sample sample in advance.

As the porous membrane 14 is disposed in the first module 100 , since a wide membrane is present, the role of a pre-filter can be efficiently performed. In addition, it is possible to prevent a large negative pressure from being applied due to the debris stuck in the membrane 28 . Accordingly, the sample sample including the biomaterial can easily reach the membrane 28 of the second module 200 .

The pore size of the porous membrane 14 is preferably larger than the pore size of the membrane 28 to be described later.

As the porous membrane 14, a commonly used debris filter may be used.

The first module 100 has a shape in which the upper part is an opening and closing opening in order to input a sample sample. The opening/closing opening is a structure including the opening/closing lid 16 .

The lower portion of the first module 100 may have a shape inclined toward the center like a funnel shape, but is not limited thereto.

In the present invention, the sample may be used without limitation as long as it is a material that can be collected or extracted from a living body, environment, or food.

Preferably, the biological sample may include blood, plasma, serum, saliva, sputum, urine, nasopharyngeal rub, nasopharyngeal aspirate, cerebrospinal fluid, and the like.

The environmental sample may be a material collected from water, soil, or air.

The sample sample passing through the porous membrane 14 is removed from cells, foreign substances, debris and large components, and contains components to be detected, such as bacteria, viruses, antibodies, proteins, and nano-endoplasmic reticulum, which are specific biological materials.

2 is an exploded cross-sectional view of the second module 200 according to the present invention.

3 is a plan view of the first passage 22 in the second module 200 of the present invention, and FIG. 4 is a plan view of the second passage 24 in the second module 200 of the present invention.

2 to 4 , the second module 200 includes a first passage 22 and a second passage 24 .

The first passage 22 is a space through which the filtered sample sample passes, and is longer than the length of the membrane, which will be described later.

The first passage 22 is disposed in the lower direction and the vertical direction with respect to the bottom surface of the first module 100 .

A second passage 24 is disposed on a side surface of the first passage 22 .

At this time, it is preferable that the second passage 24 is disposed on the side surface of the first passage 22 , so that the end of the first passage 22 and one end of the second passage 24 are coupled.

Here, one end and one end refer to both ends of the passage. Specifically, one end refers to an area corresponding to the center to an area corresponding to the end of the length of the passage. The end refers to the area corresponding to the center to the area corresponding to the other end of the length of the passage.

The first passageway 22 and the second passageway 24 have a space for inserting the membrane 28 . Specifically, the first passage 22 includes a first groove in the outer part. The first groove may be located at an end. The second passage 24 includes a second groove in an outer part thereof. The second groove may be located at one end.

A space is formed by the groove at a portion where the first groove and the second groove are coupled, and the membrane 28 is disposed in the space. In other words, the membrane 28 and the support 30 are disposed inside the area where the side surface of the first passage 22 and the side surface of the second passage 24 contact each other.

In order to insert the membrane 28 and the support 30 , the side surface of the first passage 22 and the side surface of the second passage 24 may be joined by ultrasonic welding. The joined portion may be disassembled again if necessary.

5 and 6 , portions A1, A2, and A3 positioned in the first passage 22 are fused to portions B1, B2, and B3 positioned in the second passage 24 by ultrasonic waves.

Ultrasonic welding is a melting and welding process that is instantaneously melted by frictional heat by ultrasonic vibration. Specifically, the vibrator vibrates by the electrical signal transmitted from the ultrasonic generator. The vibration generated by the vibrator is transmitted to the A1, A2, A3 parts and the B1, B2, B3 parts. The ultrasonic generator transmits an electrical signal for generating vibration in the vibrator, and controls power and ultrasonic vibration of the vibrator.

In the ultrasonically fused portion, if the shapes of A1, A2, and A3 have grooves, the shapes B1, B2, and B3 may have protrusions.

The ultrasonically fused portion may be at least one of A1, A2, and A3, and may be at least any one of B1, B2, and B3. Preferably, there are two to three in the first passage 22, and may be two to three in the second passage 24 so that the number and position of the corresponding part correspond.

The membrane 28 is a place where the biomaterial to be detected, such as bacteria, viruses, antibodies, proteins, nano-vesicles, etc. included in the sample sample, is concentrated while the filtered sample sample passes.

The membrane 28 is longitudinally disposed inside the area where the side surface of the first passageway 22 and the side surface of the second passageway 24 contact each other. That is, the membrane 28 is disposed in a vertical direction with respect to the bottom surface of the first module 100 to collect specific components according to the flow of the sample sample.

7 is a cross-sectional view of the membrane 28 and the support 30 according to the present invention.

As shown in FIG. 7 , a structure in which the membrane 28 and the support 30 are combined is shown.

In FIGS. 7 to 9 and 14 to 16 , the structure in which the membrane 28 and the support 30 are combined is denoted by the reference numeral "26".

Here the membrane 28 is used for protein attachment.

The support 30 prevents the membrane 28 from being adsorbed toward the third module 300 by negative pressure and escaping, and has a function of supporting the membrane 28 to withstand the pressure.

The support 30 may include pores, but is not limited thereto.

The support 30 may be used without limitation as long as it serves to support the thin membrane 28 .

The membrane 28 includes micropores having a size of several nm to several μm in size, and the pore size is an important factor for concentrating the biomaterial.

The pore size of the membrane 28 may range from approximately 0.01 nm to 5 μm.

In the pore size range, there is an effect that the biomaterial is efficiently highly concentrated in the membrane 28 .

The thickness of the membrane 28 and the support 30 may be the same as each other, or the thickness of the support 30 may be relatively thicker, but is not limited thereto.

The shape of the membrane 28 and the support 30 may be a right angle or a circular shape, but is not limited thereto.

The length d ₁ of the membrane 28 and the support 30 may be approximately 5 to 20 mm, but is not limited thereto.

Each of the membrane 28 and the support 30 is made of nylon (Nylon), nitrocellulose (NC), polyester (PE), polycellponate (PS), polyester cellphone (PES), polytetrafluoroethylene (PTFE) , polyvinylidene difluoride (PVDF), polypropylene (PP), cellulose acetate (CA), regenerated cellulose (RC), glass fiber, carbon nanotube (CNT), graphene, and ceramics It may include one or more types.

As such, the membrane 28 has an effect of highly concentrating even a very small biomaterial. In addition, the membrane 28 enables high concentration of biomaterials within a short time.

In order to easily take out the membrane 28 after concentration in the biomaterial concentration device of the present invention is performed, an opening/closing window 27 may be disposed on the side surface of the second module 200 .

When the membrane 28 is taken out by opening the opening/closing window 27, the membrane 28 can be safely separated without damage or scratches.

The second module 200 further includes a valve 32 .

8 and 9 are cross-sectional views of the second module 200 according to the opening/closing control of the valve 32 of the present invention.

As shown in FIGS. 8 and 9 , the valve 32 is disposed under the first passage 22 and opens and closes by moving vertically from the lower surface of the first passage 22 to the position of the membrane 28 . is regulated

As shown in FIG. 8 , when the valve 32 moves upward to the position of the membrane 28 , the valve 32 is closed (OFF). When the valve 32 is closed, since the valve 32 blocks the front of the membrane 28 , the sample sample that has passed through the first module 100 cannot access the membrane 28 and is stored in the first passage 22 . will be as

As shown in FIG. 9 , on the contrary, when the valve 32 is located on the lower surface of the first passage 22 , the valve 32 is opened (ON). When the valve 32 is opened, the sample sample passing through the first module 100 passes through the membrane 28 and concentration proceeds.

Likewise, the valve 32 may be selectively used by opening and closing the valve 32 according to the washing solution injection step, the intermediate reaction solution treatment step, and the detection solution treatment step as well as the sample sample.

The third module 300 includes a vacuum pump 40 disposed under the second passage 24 .

A vacuum pump 40 is disposed in the third module 300 having a volume. The vacuum pump 40 applies negative pressure to the first module 100 and the second module 200 through expansion.

When a negative pressure is formed by operating the vacuum pump 40 from the contracted state (OFF) to the expanded state (ON), the sample sample filtered in the first module 100 passes through the membrane 28 of the second module 200 . will do At this time, the biological material (bacteria, virus, antibody, protein, etc.) included in the sample is attached to the membrane 28 and concentrated.

10 is a cross-sectional view showing a contracted state (OFF) of the vacuum pump 40 of the present invention.

As shown in FIG. 10 , the vacuum pump 40 discharges air to indicate a contracted state (OFF). In this case, by gravity, the sample may be attached to and passed through the membrane 28 while passing through the first passage 22 .

11 is a cross-sectional view showing an expanded state (ON) of the vacuum pump 40 of the present invention.

As shown in FIG. 11 , the vacuum pump 40 sucks air to indicate an expanded state (ON).

When only concentration is performed, it is possible to proceed with concentration in the state shown in FIG. 11 . In case of direct diagnosis without separating each module when proceeding step by step, the length at which the stopper is pulled can be adjusted step by step as steps such as washing, blocking, and reagent reaction are added for each dose.

By the suction force by the vacuum pump 40 together with gravity, the sample sample may be attached to and passed through the membrane 28, and the concentration efficiency of the biological material may be improved. In addition, it is possible to concentrate very quickly.

In order to show the efficiency of concentration of the biological material, it is preferable that the volume of the vacuum pump 40 is large. For this purpose, it is preferable that the volume of the third module 300 is at least one time greater than the volume of the first module 100 .

The magnitude of the negative pressure formed by the vacuum pump 40 may be adjusted according to the stopper 42 . With respect to the vacuum pump 40 , as the stopper 42 is pulled downward, the volume of the vacuum pump 40 becomes the maximum value.

The stopper 42 has a bar shape. The stopper 42 is disposed in the longitudinal direction under the vacuum pump 40 and penetrates the bottom surface of the third module 300 .

And the stopper 42 has a plurality of locking projections 44 are arranged at regular intervals on the outer peripheral surface.

When negative pressure is formed according to the vacuum pump 40 , the stopper 42 moves downward. When the concentration step, the intermediate reaction solution treatment step, etc. are sequentially performed, the pulling length of the stopper 42 may be adjusted according to the set step.

At this time, the length of the movement in the downward direction is adjusted according to the position of the plurality of locking projections (44). The locking protrusion 44 prevents the stopper 42 from moving upward, and serves to prevent a reverse flow of contaminants accumulated in the vacuum pump 40 .

To this end, the locking protrusion 44 may include an elastic material such as rubber or silicone material. When the locking protrusion 44 is made of an inelastic material such as glass, the stopper 42 cannot move downward because of the locking protrusion 44 .

12 is a cross-sectional view showing an open state of the locking projection 44 of the present invention.

As shown in FIG. 12 , the locking protrusion 44 maintains an open state.

If, as shown in FIG. 10, the stopper 42 is pulled by the indicated length (d ₂ ) while the locking protrusion 44 is located inside the third module 300, the locking protrusion as much as the pulled length (d ₂ ) ( 44) is located outside the third module 300 .

Since the locking protrusion 44 maintains an open state, the stopper 42 does not move back into the third module 300 .

13 is a cross-sectional view showing a state in which the locking projection 44 of the present invention is closed.

As shown in FIG. 13 , when the locking protrusion 44 is closed, it is located on the bottom surface of the third module 300 .

As such, the disposable biomaterial concentration device of the present invention has the advantage that the first module, the second module and the third module are integrally combined, and it is light and easy to handle.

The biomaterial is concentrated only on the membrane disposed in the vertical direction on the second module.

In particular, since the sample sample passes only in one direction, there is an effect of easy disposal without a phenomenon in which the contaminants accumulated in the third module are discharged to the outside or flow backward in the upward direction.

In addition, it is possible to prevent contamination of the membrane in which the biomaterial is concentrated from the outside.

Furthermore, in the present invention, detection and diagnosis are possible by recovering only the second module in which the biomaterial is concentrated, and it can also be used in a method of testing a gene.

The operation process of the biomaterial concentrating device of the present invention will be described in detail as follows.

14 is an operation view when the valve 32 and the vacuum pump 40 are OFF in the biomaterial concentrating device of the present invention.

In FIG. 14 , the structure in which the membrane 28 and the support 30 are combined is denoted as 26 .

The operation state of FIG. 14 may be a state before the sample sample is input.

15 is an operation view when the valve 32 is OFF and the vacuum pump 40 is ON in the biomaterial concentrating device of the present invention.

In FIG. 15 , the structure in which the membrane 28 and the support 30 are combined is denoted as 26 .

15 , only the valve 32 is in a closed state, and the material injected according to the steps may not adhere to the membrane 28 coupled to the support 30 .

16 is an operation view when the valve 32 and the vacuum pump 40 are ON in the biomaterial concentrating device of the present invention.

In FIG. 16 , the structure in which the membrane 28 and the support 30 are combined is denoted as 26 .

In the operation of FIG. 16 , the filtered sample sample passes through the membrane 28 after the sample sample is injected through the first module 100 . At this time, the biological material included in the sample sample is highly concentrated while passing through the innumerable pores of the membrane 28 . The remaining components and contaminants other than the biological material flow down through the second passage 24 and accumulate in the vacuum pump 40 of the third module 300 .

For example, when the valve 32 is ON and the vacuum pump 40 is ON, urine is filtered through the porous membrane 14 . As the filtered urine passes through the membrane 28 , proteins and the like contained in the urine are attached to the membrane 28 . Thereafter, when another sample is injected into the porous membrane 14 and reacted, the detection protein present in the sample is also attached to the membrane 28 .

In order to prevent such adhesion, unreacted proteins may be passed through the membrane 28 . Accordingly, it is possible to minimize the non-specific reaction in the next step, the detection action.

In the detection solution treatment step, Gold-Ab or fluorescence-Ab may be used.

Gold-Ab is a reagent mainly used in the detection method, and in case of a positive reaction, it is displayed in red.

The detection reagent may be stored in another container, and only the membrane 28 or the second module 200 on which the membrane 28 is disposed is separated and then reacted with the detection reagent to perform detection. In addition, by extracting the gene, it is possible to detect pathogens present by PCR, RT-PCR, sequencing, and the like.

Alternatively, detection may be performed by injecting and reacting a detection reagent that specifically reacts with the biological material concentrated in the membrane 28 into the first module 100 and the second module 200 disposed in the concentration device.

17 is a cross-sectional view of an example in which the third module 300 is disposed in a vertical direction with respect to the second module 200 in the biomaterial concentrating apparatus of the present invention.

In FIG. 17 , the structure in which the membrane 28 and the support 30 are combined is denoted as 26 .

As shown in FIG. 17 , in the apparatus for concentrating biomaterials according to another embodiment of the present invention, the upper part is an opening and closing opening, and a porous membrane for filtering a sample sample is disposed in a first module 100 , the first module 100 ) a first passage disposed in a vertical direction with respect to the bottom surface, a second passage disposed on a side surface of the first passage, and a membrane disposed inside an area where the side surface of the first passage and the side surface of the second passage contact A second module 200 including, and disposed on the side surface of the second passage 24, disposed in a vertical direction with respect to the side surface of the second passage 24, the first module 100 and the second and a third module 300 including a vacuum pump for applying a negative pressure to the module 200 .

In particular, in the structure in which the third module 300 is disposed in a vertical direction with respect to the side surface of the second module 200, the end (lower region) of the second passage has an “L” shape and is formed with the third module 300 and It has a connected structure.

The first module, the second module, and the vacuum pump are the same as described above.

As described above, the present invention has been described with reference to the illustrated drawings, but the present invention is not limited by the embodiments and drawings disclosed in this specification, and various methods can be obtained by those skilled in the art within the scope of the technical spirit of the present invention. It is obvious that variations can be made. In addition, although the effects according to the configuration of the present invention have not been explicitly described and described while describing the embodiments of the present invention, it is natural that the effects predictable by the configuration should also be recognized.

100: first module
200: second module
300: third module
12: connecting passage
14: porous membrane
22: first passage
24: second passage
27: open and close window
28: membrane
30: support
32: valve
40: vacuum pump
42: stopper
44: snag

Claims (10)

a first module having an upper opening and closing opening and having a porous membrane for filtering a sample sample;
A first passage disposed in a vertical direction with respect to the bottom surface of the first module, a second passage disposed on a side surface of the first passage, and an area in which the side surface of the first passage and the side surface of the second passage contact a second module comprising a membrane; and
a third module disposed under the second passage and including a vacuum pump for applying negative pressure to the first module and the second module; and
A biological material concentrating device in which the biological material contained in the sample sample is concentrated on the membrane.
According to claim 1,
The second passage is disposed on the side of the first passage, the biomaterial concentration device is arranged so that the end of the first passage and the end of the second passage are coupled.
According to claim 1,
and a valve disposed under the first passage and configured to open and close by moving in a vertical direction from the lower surface of the first passage to the location of the membrane.
According to claim 1,
The membrane is a biological material concentrating device disposed in the longitudinal direction therein.
5. The method of claim 4,
The membrane is made of nylon (Nylon), nitrocellulose (NC), polyester (PE), polycellponate (PS), polyester cellphone (PES), polytetrafluoroethylene (PTFE), polyvinylidene difluoride ( PVDF), polypropylene (PP), cellulose acetate (CA), regenerated cellulose (RC), glass fiber, carbon nanotubes (CNT), graphene (graphene) and biomaterials including at least one of ceramic concentrating device.
According to claim 1,
The volume of the third module is at least 1 times greater than the volume of the first module or more biological material concentrating device.
According to claim 1,
A biological material concentrating device comprising a stopper disposed in the longitudinal direction at the lower portion of the vacuum pump and having locking projections disposed on an outer circumferential surface at regular intervals.
8. The method of claim 7,
The stopper moves in the lower direction, but the length of moving in the lower direction is controlled according to the position of the locking protrusion.
According to claim 1,
The first module, the second module and the third module are integrated biomaterial concentration device.
a first module having an upper opening and closing opening and having a porous membrane for filtering a sample sample;
A first passage disposed in a vertical direction with respect to the bottom surface of the first module, a second passage disposed on a side surface of the first passage, and an area in which the side surface of the first passage and the side surface of the second passage contact a second module comprising a membrane; and
A third module disposed on the side surface of the second passage, disposed in a vertical direction with respect to the side surface of the second passage, and including a vacuum pump for applying a negative pressure to the first module and the second module; and
A biological material concentrating device in which the biological material contained in the sample sample is concentrated on the membrane.

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