KR20220015013A - Composition comprising Lespedeza plant extracts for skin volume augmentation - Google Patents

Abstract

The present invention relates to a composition comprising a Lespedeza plant extract for enhancing the skin volume, and more specifically, to a cosmetic composition including, as an active component, an extract of Lespedeza cuneata, Lespedeza capitata or Lespedeza bicolor which are Lespedeza plants for restoration or expansion of the skin volume. The composition comprising, as an active component, the extract of Lespedeza cuneata, Lespedeza capitata or Lespedeza bicolor, according to the present invention, can promote the proliferation of adipocytes and differentiation into adipocytes so as to form a subcutaneous layer or provide fat cells, thereby increasing adipose tissues. Accordingly, the volume of skin tissues that are depressed or need to be expanded can be restored and expanded. Therefore, the composition comprising, as an active component, a Lespedeza plant extract for the proliferation of adipocytes and differentiation into adipocytes can be usefully employed as a cosmetic composition for restoration and expansion of the skin volume, a skin external application composition for increasing the skin volume, or a cosmetic surgical composition for increasing the skin volume.

Description

Composition comprising Lespedeza plant extracts for skin volume augmentation

The present invention relates to a composition for increasing skin volume comprising an extract of a plant of the genus genus, and more particularly, a plant of the genus genus Lespedeza cuneate , buciclovir ( Lespedeza capitata ) or sagebrush ( Lespedeza bicolor ) extract as an active ingredient It relates to a cosmetic composition for restoration or enlargement of the skin volume, including

In modern society, skin has become a symbolic indicator of health and beauty, and improvement of sagging skin that loses elasticity is emerging as a major concern from a social and aesthetic point of view. In particular, in the case of women, a decrease in breast skin elasticity and a decrease in fat caused by various factors, including hormonal changes, make a woman feel a great loss of femininity. and to enhance elasticity. Due to this trend of social awareness, the necessity and importance of breast firming products are being highlighted more than ever.

The subcutaneous fat layer under the dermis is composed of lobules of adipocytes, which act as a cushion to protect the internal organs of the skin against external heat and absorb shock, and contain nutrients in the form of triglyceride. It is also stored in organelles called lipid droplets and used as an energy source when necessary. However, as we age, the fat production in the subcutaneous fat decreases due to natural factors and various external stimuli, so that the original function of each component cell is not properly performed throughout the skin, resulting in a decrease in skin volume.

Fat grafting is a method currently widely used to improve the skin volume reduction. Fat grafting is a surgical procedure in which fat is harvested using a syringe with a tulip needle-shaped needle with a non-sharpened tip and transplanted into each area of the skin with reduced volume. However, it has been reported that 40-60% of the transplant volume is reabsorbed into the human body after fat grafting (S Eremia et al. , Dermatol Surg. , 26(12):1150-1158, 2000; Fulton JE et al. , Dermatol Clin. , 19(3): 523-530, 2001), Fat grafting in areas with thin skin and low subcutaneous fat raises problems such as palpable cell masses, and the survival rate and survival of the actually transplanted fat There is a problem that re-transplantation is necessary because the period does not meet expectations. Accordingly, there is an increasing demand for a composition capable of sufficiently increasing the subcutaneous fat layer while replacing the fat grafting operation having these problems.

The basic structure of the skin is maintained by subcutaneous adipose tissue, and this subcutaneous adipose tissue plays a role in determining the volume and strength of the skin. can be Therefore, studies using adipocytes are being actively conducted in various fields, and the recent attention in studies using adipocytes is the 3T3L1 pre-adipocyte. Since these preadipocytes can be differentiated into adipocytes through treatment with an inducer such as an inducible growth factor, they are widely used in the study of differentiation into adipocytes.

On the other hand, Lespedeza cuneate G. Don of the genus Lespadeza sp. belongs to the Rosaceae legume and is mainly distributed in Asia such as Korea, Japan, and Taiwan. It has the effect of controlling swelling and boils, preventing enlarged prostate, removing eohyeol, and is also effective for bronchitis, asthma, cough, and phlegm. It is also used for acute gastritis, gastric ulcer, and diarrhea. Since it contains flavonoids such as quercetin, kaempferol, and vitexin, it has various effects such as antioxidant, blood sugar lowering, and anti-inflammatory.

Bush clover ( Lespedeza capitata ) belongs to the Rosaceae legume family, and grows mainly in eastern North America. It is a perennial shrub, and in folk records of Native Americans, the roots were used as an antidote to poisoning, and the stems were used to treat neuralgia and rheumatism. In addition, it contains substances that lower blood cholesterol and nitrogen, and the stems and leaves contain flavonoids and alkaloids. As a result of a recent study, it was confirmed that the sagebrush had an effect in preventing arteriosclerosis, etc. in advance by preventing the thickening of the blood vessel wall (Ha SJ et al. , Food & Function , 9(4):2090-2101, 2018).

Lespedeza bicolor is a deciduous shrub belonging to the family Rosaceae and is mainly distributed in Korea, Japan, and China. Stems and leaves are sweet in taste, flat in nature, non-toxic, and mainly act on heart and menopause. It has been known to be effective against waste heat seawater, whooping cough, nosebleeds, and bedridden disease. It is collected in summer and autumn, and fresh ones are used as they are or cut and dried in the sun. The roots are collected from April to October, and are effective in treating heartburn, back pain, rheumatoid muscle and joint pain, bruises, red white scabies, and eucalyptus poison. However, there has been no report on the promotion of adipocyte proliferation and adipocyte differentiation in plants of the genus Sophia.

As a result, the present inventors studied a composition that can sufficiently increase the subcutaneous fat layer while replacing fat grafting surgery. By confirming that it was created, the present invention was completed.

S Eremia et al., Dermatol Surg., 26(12):1150-1158, 2000 Fulton JE et al., Dermatol Clin., 19(3): 523-530, 2001 Ha SJ et al., Food & Function, 9(4):2090-2101, 2018

It is an object of the present invention to restore or restore the skin volume comprising at least one selected from the group consisting of Lespedeza cuneate , buciclovir ( Lespedeza capitata ) and Lespedeza bicolor ( Lespadeza sp.) plant extract as an active ingredient. It is to provide a cosmetic composition for enlargement.

Another object of the present invention is to provide a composition for external application for skin application for increasing skin volume comprising, as an active ingredient, one or more plant extracts of the genus genus Bisuri selected from the group consisting of bisuri, buciclovir and thyme.

Another object of the present invention is to provide a cosmetic composition for increasing skin volume comprising, as an active ingredient, one or more extracts of plants of the genus genus Bisuri selected from the group consisting of bisuri, buciclovir and thyme.

In order to achieve the above object, the present invention is bisuri ( Lespedeza cuneate ), buciclovir ( Lespedeza capitata ) and sagebrush ( Lespedeza bicolor ) At least one selected from the group consisting of ( Lespadeza sp. ) A plant extract comprising a plant extract as an active ingredient Provided is a cosmetic composition for restoring or enlarging skin volume.

In addition, the present invention provides a composition for external application for skin for increasing skin volume comprising, as an active ingredient, one or more plant extracts of the genus Psoriasis selected from the group consisting of bisuri, buciclovir and sari.

In addition, the present invention provides a cosmetic composition for increasing the volume of the skin comprising as an active ingredient at least one plant extract selected from the group consisting of bisuri, buciclovir, and ssiri.

The composition comprising an extract of Lespedeza cuneate , Lespedeza capitata or Lespedeza bicolor as an active ingredient, which is a plant of the genus of the present invention, constitutes the subcutaneous fat layer by promoting the proliferation of adipocytes and differentiation into adipocytes. Alternatively, it is possible to repair and enlarge the volume of skin tissue that is depressed or needs enlargement by providing fat cells to increase adipose tissue. Therefore, the composition for promoting the proliferation and differentiation of adipocytes and the differentiation into adipocytes comprising the plant extract of the genus Pseudomonas according to the present invention as an active ingredient is a cosmetic composition for skin volume restoration and enlargement, a skin external composition for increasing skin volume, or skin It can be usefully used as a composition for molding for increasing volume.

1 is a graph showing the quantification of cell proliferation of 3T3-L1 pre-adipocytes by extracts of the genus Psoriasis ( Lespedeza cuneate ), buciclovir ( Lespedeza capitata ), and lichen ( Lespedeza bicolor ) of the present invention.
Figure 2 is a graph showing the numerical value of the cell proliferation of adipocytes by the extracts of non-suri, buciclovir, and prickly pear of the present invention.
3 is a result of observing the promotion of adipocyte differentiation of 3T3-L1 preadipocytes by the extracts of non-suri, buciclovir, and prickly pear of the present invention by using Oil-red O staining method.
4 is a graph showing the quantification of the promotion of adipocyte differentiation of 3T3-L1 preadipocytes by the extracts of non-suri, buciclovir and P. sirius of the present invention.

As an aspect, the present invention provides a skin volume comprising at least one plant extract selected from the group consisting of Lespedeza cuneate , buciclovir ( Lespedeza capitata ) and Lespedeza bicolor as an active ingredient. It provides a cosmetic composition for restoration or enlargement of

As used herein, the term “skin” refers to a tissue covering the body surface of an animal, and is the broadest concept including tissues covering the body surface, such as a face or body.

In the present invention, the genus plant is not necessarily limited thereto, but is one or more plants selected from the group consisting of non-superior, buciclovir, and cypress.

In the present invention, the use part of the plant of the genus S. genus may use leaves, flowers, roots, stems, seeds, and all or above-ground parts thereof, preferably, the above-ground part or above-ground parts and roots of the genus S. .

In the present invention, the composition is characterized in that it promotes the proliferation of adipocytes and differentiation into adipocytes by using a plant extract of the genus Pseudomonas, specifically, extracts of Bisuri, buciclovir and Psoriasis as active ingredients.

As used herein, the term "adipocyte proliferation" refers to an increase in the number of adipocytes in an animal, preferably a mammal, and "differentiation of adipocytes" refers to an increase in the number of adipocytes in an animal, preferably a mammal. differentiation into adipocytes.

The plant extract of the genus Pseudomonas of the present invention can be used to construct a subcutaneous fat layer by promoting the proliferation and differentiation of adipocytes, or to repair and enlarge the volume of skin tissue that is depressed or needs enlargement by providing adipocytes to increase adipose tissue. can For example, it is possible to restore or enlarge the volume of the skin that is congenitally depressed, or is dented by an accident or surgery, or has a wound. In addition, for cosmetic reasons, areas that require an increase in adipose tissue, for example, face, such as nose, cheekbones, cheeks, forehead, nasolabial folds, and under eyes, as well as body parts such as chest and buttocks, are repaired and enlarged. can do it

The plant extract of the genus Psoriasis of the present invention can form a subcutaneous fat layer by promoting the proliferation of adipocytes and differentiation into adipocytes, or provide adipocytes to improve skin wrinkles and increase skin elasticity.

The composition of the present invention may be used as a cosmetic composition for restoring and enlarging skin volume, an external composition for skin application for increasing skin volume, or a composition for molding for increasing skin volume.

Angiosperm plants used in the present invention is a genus of angiosperm legumes ( Leguminosae ). It has about 30 species, and there are bushes and grasses. It is native to temperate and subtropical regions of eastern North America, East Asia, Southeast Asia, and Australia. Lespadeza cuneate and Bush clover ( Lespedeza capitata ) are perennial woody herbs, with straight stems and thin, short branches with ridges and hairs. The leaves are alternate and are compound leaves with three small leaves. The leaves, roots, and stems are all edible, and it contains flavonoids, tannins, and phenols, so it has anti-inflammatory effects. Lespedeza bicolor is a broad-leaved shrub with fallen leaves, and the height is about 1-2 m. The branches are not straight but curved, and the leaves are three-leaved, opposite, and have petioles. The raceme that hangs on the leaf is longer than the leaf, unlike the sagebrush. The small leaves are round and egg-shaped, with a smooth edge and a length of 2-4 cm. Flowers bloom in late summer, and small red-purple butterfly flowers form raceme inflorescences. The fruit has a flattened shape, and one seed is made inside. It is mainly found in mountainous areas and is widely distributed throughout Korea. Overseas, it is distributed in Japan, the northeastern part of China, and the Ussuri region. Flowers bloom in July-August.

The term "extract" used in the present invention refers to a preparation concentrated by squeezing a herbal medicine with an appropriate leaching solution and evaporating the leachate, but is not limited thereto, but is not limited thereto, but is not limited to an extract obtained by extraction, a diluted or concentrated solution of the extract, or an extract. It may be a dried product obtained by drying, a crude product or a purified product thereof.

In the present invention, the extract can be prepared using general extraction methods, separation and purification methods known in the art. As the extraction method, a method such as hot water extraction, hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction may be used, but is not limited thereto.

The plant extract of the genus Pseudomonas of the present invention can be extracted using a conventional extraction solvent known in the art, and the extracted liquid can be used in liquid form, or concentrated and/or dried. In this case, the extraction solvent is, for example, (a) water, (b) an anhydrous or hydrous lower alcohol having 1-4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) a mixture of the lower alcohol and water The solvent, (d) acetone, (e) ethyl acetate, (f) chloroform, or (g) 1,3-butylene glycol can be obtained as an extraction solvent. Preferably, extraction is performed using methanol, ethanol or butanol. Depending on the extraction solvent, the degree of extraction and loss of the active ingredient of the extract may be different, so an appropriate extraction solvent should be selected and used. The extraction method is not particularly limited, and for example, cold extraction, ultrasonic extraction, reflux cooling extraction, and the like.

In addition, the plant extract of the genus Pseudomonas can be obtained through a conventional purification process in addition to the method of extraction with the extraction solvent. For example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. obtained through various additional purification methods It can also be obtained through fractionation.

In one specific implementation, the plant extract of the genus genus can be prepared by collecting the above-ground parts and root parts of the genus genus, washing and drying in the shade, and then immersing the pulverized plant powder of the genus genus in ethanol, ultrasonically extracting it, and filtering.

In the present invention, the "active ingredient" refers to a component that can exhibit the desired activity alone or can exhibit activity together with a carrier that does not have the activity itself.

In the composition of the present invention, the plant extract of the genus Psoriasis may be included in an amount of 0.00001 to 15% by weight, more preferably 0.0001 to 10% by weight, and most preferably 0.001 to 5% by weight, based on the total weight of the composition. At this time, if the content of the extract of Sasiris genus is less than 0.00001% by weight, the effect of differentiation into adipocytes, which is the objective effect of the present invention, cannot be obtained, and if it exceeds 15% by weight, the effect is not proportional as the content increases, so it may be inefficient. And there is a problem that the stability of the formulation is not secured.

On the other hand, the composition comprising the plant extract of the genus Pseudomonas of the present invention as an active ingredient can form a subcutaneous fat layer by promoting the proliferation and differentiation of adipocytes, or restore and enlarge the skin volume by providing adipocytes. In addition, as well as giving volume and elasticity to the skin and improving wrinkles, as it is derived from a natural substance, it has almost no cytotoxicity.

In the present invention, the ingredients included in the "cosmetic composition" may include ingredients commonly used in cosmetic compositions in addition to the active ingredient as an active ingredient, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments and customary adjuvants such as perfumes, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether as carrier components Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester may be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

As another aspect, the present invention provides an external composition for skin application for increasing skin volume, comprising, as an active ingredient, an extract of one or more plants selected from the group consisting of Bisuri, buciclovir, and Sari.

The term "volume" used in the present invention refers to the volume, volume, or volume of the surface or interior of the skin layer, and may mean a sense of volume exposed through the outer surface. Thus, in the case of skin with increased volume or increased volume, the external appearance will be firm, healthy and youthful.

In addition, in the case of damage to the skin due to trauma, wounds or burns caused by external force, scars, dents on the outer surface of the skin, or incisions in the skin or tissue due to surgery, etc. When the volume is increased, the volume of the tissue and the skin layer of the damaged part is increased, and the skin or tissue is restored to have an effect of approaching the original state.

As used in the present invention, the term "increased volume" means that the volume of the skin layer increases and increases, or the shape of the outer surface of the skin improves and tightens to look healthy, or damaged skin tissue is restored to its original state. may mean getting closer to Therefore, when the volume of the skin tissue is increased, the volume of the part of the skin layer that has been stretched increases and the skin surface is stretched together. can

In the present invention, the composition for external application for skin can enhance the volume and elasticity of the face and body by promoting the differentiation of fat cells. When the composition for external application for skin of the present invention is applied to a part of the face and body where volume and elasticity enhancement is required, volume and elasticity are enhanced. Areas that require volume and elasticity enhancement may be areas that are depressed due to aging. For example, there may be mentioned cheekbones that are depressed due to aging. When the skin external composition of the present invention is applied to the depressed cheeks, the volume can be increased by promoting the differentiation of adipocytes. In addition, as another example of a region requiring enhancement of volume and elasticity, the chest and buttock regions may be cited. When the composition for external application for skin of the present invention is applied to the chest and buttocks, there is an effect of making the chest and buttocks elastic and full.

In addition, the composition for external application for skin of the present invention can improve the lines of the face and body. Bisuri, buciclovir or ssiri extract contained as active ingredients promotes the differentiation of fat cells, thereby enhancing the volume and elasticity of the face and body, thereby improving the lines of the face and body damaged due to causes such as aging.

When the composition according to the present invention is used as a composition for external application for skin, it is not particularly limited in its formulation depending on body parts. Specifically, for example, it may be a transdermal dosage form such as a lotion, ointment, gel, cream, patch or spray.

The composition for external application for skin according to the present invention contains Bisuri, buciclovir, or Psoriasis extract as an active ingredient in an amount of 0.00001 to 15% by weight, more preferably 0.0001 to 10% by weight, and most preferably 0.001 to 5% by weight, based on the total weight of the composition for external application for skin. % can be included.

In addition, in the composition for external application for skin according to each formulation, a person skilled in the art can appropriately select and mix ingredients other than the above-mentioned Bisuri, buciclovir, or Sari extract of the present invention according to the formulation or purpose of use of other external preparations for skin without difficulty. In this case, a synergistic effect may occur when applied simultaneously with other raw materials.

Specifically, the present invention was the first to discover that, during the differentiation of 3T3-L1 cells, which are adipocytes, into adipocytes, a plant extract of the genus Pseudomonas, specifically, an ethanol extract of Bisuri, buciclovir, or Psoriasis, promotes differentiation into adipocytes. , based on this, provides a composition for external application for skin for increasing skin volume to which an ethanol extract of Bisuri, buciclovir or Sari is added.

The composition for external application for skin of the present invention has the advantage of not only providing elasticity and volume to a woman's breast at the same time, but also having an excellent bust firming effect by activating the fat cells constituting the subcutaneous fat layer or forming most of the breast.

In addition, the use of the ethanol extract of Bisuri, buciclovir or ssiri of the present invention is not limited to the bust firming effect of the chest region, but as described above, a new concept of skin aging prevention function and elasticity enhancement for aging and loss of elasticity skin There is an advantage that can be applied to the manufacture of functional cosmetics.

As another aspect, the present invention provides a cosmetic composition for increasing skin volume comprising, as an active ingredient, one or more plant extracts of the genus Pseudomonas selected from the group consisting of nonsuri, buciclovir, and Sari.

The application site of the cosmetic or cosmetic composition for promoting differentiation of adipocytes according to the present invention includes all adipose tissue of the body, and is not limited to a specific site, for example, breast and buttocks of a woman with a lot of fat. may include In addition, the effect of increasing the adipose tissue includes an effect of increasing adipose tissue for cosmetic or cosmetic purposes, but is not limited thereto.

According to one embodiment of the present invention, there is provided a method for restoring and enlarging the volume of the skin using an extract of bisuri, buciclovir or sirius.

In addition, according to one embodiment of the present invention, there is provided a method for improving skin wrinkles and increasing skin elasticity by using a non-suri, buciclovir or siri extract.

In the present invention, the composition comprising, as an active ingredient, a plant extract of the genus Pseudomonas, which promotes the proliferation and differentiation of adipocytes, is used for repair and enlargement of skin depressions or wounds, or for cosmetic or cosmetic enlargement of volume. can be used for The site of application of such a composition may include any adipose tissue of the body. For example, it may include not only a face such as a forehead, nose, cheeks, cheekbones, nasolabial region, under the eyes, but also breasts and buttocks of a woman with a lot of fat.

In addition, the composition comprising, as an active ingredient, a plant extract of the genus Psoriasis, which promotes the proliferation of adipocytes and differentiation into adipocytes, of the present invention can be used to improve skin wrinkles and increase skin elasticity.

On the other hand, the composition comprising the plant extract of the genus Pseudomonas of the present invention as an active ingredient is derived from a natural substance, is harmless to the human body, and has almost no toxicity and side effects, so it can be used with confidence even when used for a long period of time. It can be safely applied to a composition for external use on the skin or a composition for cosmetic use.

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

Example 1: Preparation of plant genus Bisuri, buciclovir and extract

After cleaning the above-ground parts and roots of the genus Psoriasis ( Lespedeza cuneate ), buciclovir ( Lespedeza capitata ), and lichen ( Lespedeza bicolor ), dry them in the shade at 20°C to 35°C, and then each so that the particle size is 1 mm or less. crushed. After that, 100 g of the pulverized plant bisusu, buciclovir, and 100 g of the powder of the Psoriasis were immersed in a 70% ethanol solvent, and then ultrasonically extracted for 48 hours. Then, the obtained extract was filtered using filter paper (Advantec, No. 2), respectively. The filtrate was concentrated under reduced pressure to prepare ethanol extracts of nonsuri, buciclovir, and sari.

Example 2: Effect of proliferating adipocytes and adipocytes of non-suuri, buciclovir, and extracts of Psoriasis plant

In order to investigate the effect of adipocyte proliferation of ethanol extracts of Bisuri, buciclovir, and Psoriasis plant, the following experiment was carried out. Adipocytes (3T3-L1) were purchased from ATCC (American Tissue Culture Collection, USA) and cultured in DMEM medium supplemented with 10% BCS (Bovine Calf Serum). In order to differentiate into adipocytes, 1 × 10 ⁵ cells per well were inoculated in a 6-well plate with DMEM medium containing 10% BCS, and then the cells were confluent in the wells under 5% CO ₂ and 37°C conditions. Incubated until growth. When the cells were grown in a saturated state, additional culture was performed for 2 days, and MDI (isobutylmethylxanthin 0.5 mM, dexamethason 1 μM, insulin 1 μg/mL) cocktail was treated. . After 3 days, it was replaced with a medium containing insulin 1 μg/mL, and again after 3 days, it was replaced with a DMEM medium containing 10% FBS. After replacing the differentiated adipocytes with serum-free DMEM medium, each of the ethanol extracts of the genus Psoriasis, buciclovir, and Psoriasis of Example 1 were treated at different concentrations, and then cultured again for 72 hours. On the other hand, undifferentiated preadipocytes were also treated with each concentration of non-suri, buciclovir, and ethanol extracts of the genus Psoriasis, and then cultured for 72 hours. After culturing for 72 hours, it was exchanged with DMEM medium containing 1 μg/mL MTT and reacted for 3 hours. After the reaction, the medium was removed, 1 mL of DMSO was put into each well to dissolve the formazan formed in the cells, and the absorbance was measured at 570 nm.

As a result, it was confirmed that the proliferation of pre-adipocytes (3T3-L1 fibroblast) and adipocytes (3T3-L1 adipocyte) was promoted by each of the ethanol extracts of Psoriasis, buciclovir, and Psoriasis (Fig. 1 and Fig. 2). .

Example 3: Adipocyte differentiation-promoting effect of nonsuri, buciclovir, and extracts of the genus Psoriasis plant

In order to examine the adipocyte differentiation-promoting effect of ethanol extracts of Bisuri, buciclovir, and Psoriasis plant, the following experiment was carried out. It was differentiated into adipocytes as in Example 2, and each extract was treated with the MDI cocktail from the point of inducing differentiation, and then re-treated at the same concentration whenever the culture medium was replaced. The amount of fat accumulated in the differentiated cells was stained by Oil Red O staining method, dissolved with isopropyl alcohol, and absorbance (Optical Density) was measured at 540 nm.

As a result, it was confirmed that when the immature adipocytes 3T3-L1 were treated with MDI, differentiation into adipocytes was induced and intracellular lipids were accumulated. It was shown that the ethanol extract of S. sari promotes the accumulation of lipids in cells ( FIGS. 3 and 4 ).

Example 4: Safety confirmation experiment on human skin of the plant genus Bisuri, buciclovir and extract

Example 4-1: Preparation of external preparations for skin including bisusu, buciclovir, and extract

As confirmed in Example 3, in order to confirm that the ethanol extracts of Psoriasis, buciclovir and Psoriasis, which were found to be excellent in promoting effect on adipocyte lipid production, are safe for human skin, Psuridae bisusu, buciclovir and Ethanol An external preparation for skin containing the extract was prepared and a skin safety verification experiment was performed by this.

An external preparation for skin containing the plant bisusu, buciclovir, and ethanol extract of Psoriasis was prepared with the content of ingredients shown in Table 1 below.

For the preparation of external preparations for skin, purified water, glycerin, and butylene glycol were mixed and dissolved at 70° C. (aqueous part), and the remaining components except for the three components and trimethanolamine were dissolved at 70° C. (oil phase part). The oil part was added to the water part, and the mixture was first emulsified by stirring with a homomixer (Tokushu Kika, Japan), and trimethanolamine was finally added thereto. After removing the bubbles generated in the mixed solution, the mixture was cooled to room temperature to prepare an external preparation for skin.

ingredient content (%) control test group Purified water 72 71 glycerin 8.0 8.0 butylene glycol 4.0 4.0 Pseudomonas Plant Extract (70% EtOH) 0 1.0 Caprylic/Capri Triglycerides 8.0 8.0 squalane 5.0 5.0 Cetearyl Glucuside 1.5 1.5 Sorbitan Stearate 0.4 0.4 cetearyl alcohol 1.0 1.0 trimethanolamine 0.1 0.1 gross weight 100 100

Example 4-2: cumulative skin irritation test

By applying each of the external skin preparations prepared in Example 4-1 to the upper forearm area for a total of 9 24 hour cumulative patches every other day for 30 healthy adults using each of the external skin preparations prepared in Example 4-1, the ethanol extract Whether or not it irritates the skin was measured.

The patching method used a pin chamber (Finn chamber, Epitest Ltd, Finland). After 15 μl of each of the above skin external preparations was dripped into the chamber, patching was performed. The degree of the reaction shown on the skin each time was scored using the following [Equation 1], and the results are shown in Table 2 below.

[Equation 1]

Average reactivity = [(response index × reactivity / total number of subjects × highest score (4 points)) × 100)] ÷ (number of tests) (9 times)

At this time, in the reactivity, ± is given a score of 1 point, + is 2 points, and ++ is 4 points, and when the average reactivity is less than 3, it was judged as a safe composition.

test substance Number of subjects who responded average reactivity 1 week 2 weeks 3 weeks Primary Secondary tertiary 4th 5th 6th 7th 8th 9th ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ control - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.00 Test group (non-repair) - - - One - - One - - - - - - - - - - - - - - - - - - - - 0.19 Test group (buciclovir) - - - - - - One - - - - - - - - - - - - - - - - - - - - 0.09 Test group (Sari) One - - One - - One - - - - - - - - - - - - - - - - - - - - 0.28 number of people tested 30 30 30 30 30 30 30 30 30

In the case of the test group in Table 2, the number of persons corresponding to ±, +, and ++ was 1, 0, and 0, respectively, and the rest did not respond. If calculated according to Equation 1 described above, each [(1×2)/(30×4)]×100/9=0.19, [(1×1)/(30×4)]×100/9=0.09, [(1×3)/(30×4)]×100/9=0.28, with an average reactivity of 0.13 and less than 3, which was judged to be a safe composition.

As described in Table 2 above, the external skin preparations containing the plant genus Bisuri, buciclovir and ethanol extract (test group) of the genus Psoriasis did not show a distinct cumulative irritation pattern like the external skin preparation in the control group, and were judged to be safe substances for human skin. became

Formulation Example 1: Preparation of cosmetics

Formulation Example 1-1: Preparation of softening lotion

As shown in Table 3 below, a softening lotion containing an ethanol extract of Bisuri, buciclovir, or Saari, which is a plant of the genus Psoriasis, as an active ingredient was prepared according to a conventional method.

ingredient weight% genus plant extract 0.01 glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 carboxyvinyl polymer 0.1 ethanol 10.0 triethanolamine 0.1 Preservatives, trace colors, trace fragrances and trace purified water 82.79 sum 100.0

Formulation Example 1-2: Preparation of nutrient lotion

As shown in Table 4 below, a nutrient lotion containing an ethanol extract of Bisuri, buciclovir, or Saari, which is a plant of the genus Psoriasis, as an active ingredient was prepared according to a conventional method.

ingredient weight% genus plant extract 0.01 beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 liquid paraffin 5.0 squalane 5.0 Caprylic/Capric Triglycerides 5.0 glycerin 3.0 butylene glycol 3.0 propylene glycol 3.0 carboxyvinyl polymer 0.1 triethanolamine 0.2 Preservatives, micro pigments, micro fragrances and micro-purified water 69.69 sum 100.0

Formulation Example 1-3: Preparation of nutritional cream

As shown in Table 5 below, a nourishing cream containing ethanol extracts of Bisuri and buciclovir as an active ingredient, which are plants of the genus Psoriasis, was prepared according to a conventional method.

ingredient weight% genus plant extract 0.01 beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 liquid paraffin 10.0 squalane 5.0 Caprylic/Capric Triglycerides 5.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Preservatives, micro pigments, micro fragrances and micro-purified water 56.79 sum 100.0

Formulation Example 1-4: Preparation of massage cream

As shown in Table 6 below, a massage cream containing Bisuri, buciclovir, or ethanol extract of Psoriasis plant as an active ingredient was prepared according to a conventional method.

ingredient weight% genus plant extract 0.01 beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 liquid paraffin 40.0 squalane 5.0 Caprylic/Capric Triglycerides 4.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Preservatives, micro pigments, micro fragrances and micro-purified water 27.49 sum 100.0

Formulation Example 1-5: Preparation of pack

As shown in Table 7 below, a pack containing an ethanol extract of Bisuri, buciclovir or Psoriasis, which are plants of the genus Psoriasis, as an active ingredient was prepared according to a conventional method.

ingredient weight% genus plant extract 0.01 polyvinyl alcohol 13.0 Sodium Carboxymethylcellulose 0.2 allantoin 0.1 ethanol 5.0 nonylphenyl ether 0.3 Preservatives, micro pigments, micro fragrances and micro-purified water 81.39 sum 100.0

Formulation Example 2: Preparation of a pharmaceutical formulation

Formulation Example 2-1: Preparation of powder

As shown in Table 8 below, it was prepared by filling an airtight bag with a powder mixed with Bisuri, buciclovir, or ethanol extract of Psoriasis plant as an active ingredient, according to a conventional method.

ingredient content (g) genus plant extract 2 lactose One

Formulation Example 2-2: Preparation of tablets

As shown in Table 9 below, tablets containing Bisuri, buciclovir, or ethanol extract of Psoriasis, which are plants of the genus Psoriasis, as an active ingredient were prepared by tableting according to a conventional method.

ingredient Content (mg) genus plant extract 100 corn starch 100 lactose 100 Magnesium Stearate 2

Formulation Example 2-3: Preparation of capsules

As shown in Table 10 below, capsules containing ethanol extracts of Bisuri, buciclovir or Psoriasis, which are plants of the genus Psoriasis, as an active ingredient were prepared by filling in gelatin capsules according to a conventional method.

ingredient Content (mg) genus plant extract 100 corn starch 100 lactose 100 Magnesium Stearate 2

Formulation Example 3: Preparation of external preparation for skin

Formulation Example 3-1: Preparation of gel

As shown in Table 11 below, a gel containing an ethanol extract of Bisuri, buciclovir, or Psoriasis, which are plants of the genus Psoriasis, as an active ingredient was prepared according to a conventional method.

ingredient weight% genus plant extract 1.00 Butylene Glycol 2.00 glycerin 1.00 Disodium EDIT 0.02 PEG-60 Hydrogenated Castor Oil 1.00 Cocamidopropyl Betaine 4.00 Lauryl Glucoside 6.00 Polysorbate 20 1.00 Sodium Benzoate 0.40 caprylic glycol 0.20 sodium lactate 0.50 Hydroxyethyl Cellulose 0.7 Purified water 82.18 sum 100.0

Formulation Example 3-2: Preparation of ointment

As shown in Table 12 below, an ointment containing an ethanol extract of Bisuri, buciclovir or Psoriasis, which is a plant of the genus Psoriasis, as an active ingredient was prepared according to a conventional method.

ingredient weight% genus plant extract 1.0 diethanolamine 1.5 polyvinylptolidone 5.0 propylene glycol 30.0 Purified water 62.5 sum 100.0

Claims (8)

Bisuri ( Lespedeza cuneate ), buciclovir ( Leapedeza capitata ), and at least one selected from the group consisting of Lespedeza bicolor ) A cosmetic composition for restoring or enlarging skin volume comprising a plant extract selected from the group consisting of Lespadeza sp . .
The cosmetic composition according to claim 1, wherein the skin is the skin of the nose, cheeks, cheeks, forehead, nasolabial region, under the eyes, chest or buttocks.
The cosmetic composition according to claim 1, wherein the composition is for promoting adipocyte proliferation or adipocyte differentiation.
The cosmetic composition of claim 1, wherein the composition is for improving skin wrinkles.
The cosmetic composition of claim 1, wherein the composition is for increasing skin elasticity.
The cosmetic composition according to claim 1, wherein the extract is included in an amount of 0.00001 to 15% by weight based on the total weight of the composition.
Bisuri ( Lespedeza cuneate ), buciclovir ( Leapedeza capitata ) and at least one selected from the group consisting of Lespedeza bicolor ) An external composition for skin application for increasing skin volume, comprising, as an active ingredient, one or more plant extracts of the genus Lespadeza sp.
Bisuri ( Lespedeza cuneate ), buciclovir ( Leapedeza capitata ) and at least one selected from the group consisting of Lespedeza bicolor ) A cosmetic composition for increasing skin volume comprising a plant extract of Lespadeza sp. as an active ingredient.

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