KR20210109074A - Food Composition Comprising Extract of Paullinia cupana, Vitamin and Natural Extract as an Active Ingredients - Google Patents
Food Composition Comprising Extract of Paullinia cupana, Vitamin and Natural Extract as an Active Ingredients Download PDFInfo
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- KR20210109074A KR20210109074A KR1020200023411A KR20200023411A KR20210109074A KR 20210109074 A KR20210109074 A KR 20210109074A KR 1020200023411 A KR1020200023411 A KR 1020200023411A KR 20200023411 A KR20200023411 A KR 20200023411A KR 20210109074 A KR20210109074 A KR 20210109074A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Botany (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
본 발명은 과라나 추출물, 비타민 및 천연물 추출물을 유효성분으로 포함하는 식품 조성물에 관한 것이다.The present invention relates to a food composition comprising a guarana extract, a vitamin and a natural product extract as an active ingredient.
현대의 웰빙 문화의 등장이 대변하듯, 국내 소비자들의 건강에 대한 관심은 과거에 비해 매우 높아졌다. 또한, 과학의 진보가 인류의 수명 연장을 가져옴에 따라 건강기능식품은 미래 식품산업의 돌파구로 부상하게 되었다. As the emergence of modern well-being culture represents, domestic consumers' interest in health has increased significantly compared to the past. In addition, as advances in science bring extension of human lifespan, health functional food has emerged as a breakthrough in the future food industry.
1997년 650억불 규모였던 세계 기능성 식품시장은 2000년 1,380억불로의 높은 성장률은 나타내었으며, 국내 건강기능식품 시장 역시 1997년 IMF 외환 위기 시에 시장이 일시 위축되었으나, 현재까지 꾸준히 시장 규모가 성장하고 있는 추세이다.The global functional food market, which was worth 65 billion dollars in 1997, showed a high growth rate to 138 billion dollars in 2000, and the domestic health functional food market also contracted temporarily during the 1997 Asian financial crisis. there is a trend
건강기능식품 중 하나인 에너지 드링크는 운동이나 노동 등으로 인하여 체내에서 빠져나간 수분과 전해질을 보충해주어 피로회복을 돕는 기능성 드링크를 말하며, 주요 성분으로는 당(탄수화물)을 비롯하여 나트륨, 칼륨, 마그네슘 등의 무기질을 함유하고 비타민 C가 포함되기도 한다. Energy drink, one of health functional foods, refers to a functional drink that helps recover from fatigue by replenishing moisture and electrolytes lost from the body due to exercise or labor. of minerals and also contains vitamin C.
에너지 드링크는 타우린, 카페인 및 영양성분(비타민 C, 아미노산 등)이 함유되어 있어 집중력을 강화시키고 신체의 활력을 높여준다. 다만 신체의 활력을 높여주는 중심 성분이 카페인인 만큼 저녁시간대에 마시면 수면을 방해 받을 수 있는 문제가 있다. 또한, 소비자들은 에너지 드링크에 대해 건강에 안 좋은 것이라는 전반적인 선입견이 있다. Energy drinks contain taurine, caffeine, and nutrients (vitamin C, amino acids, etc.) to strengthen concentration and increase the vitality of the body. However, since caffeine is the main ingredient that increases the vitality of the body, there is a problem that sleep can be disturbed if you drink it in the evening. Also, consumers have a general preconceived notion that energy drinks are unhealthy.
따라서, 기존 에너지 드링크의 문제점을 극복하기 위한 기능성이 개선된 에너지 드링크 개발의 필요성이 있다. Therefore, there is a need to develop an energy drink with improved functionality to overcome the problems of the existing energy drink.
본 발명자들은 기능성이 개선된 에너지 드링크를 개발하고자 예의 연구 노력하였다. 그 결과 과라나 추출물, 비타민 및 천연물 추출물을 포함하는 식품 조성물의 경우 피로 회복 효과, 항산화 효과, DNA 손상 보호 효과를 나타내는 것을 규명함으로써, 본 발명을 완성하게 되었다. The present inventors made intensive research efforts to develop an energy drink with improved functionality. As a result, in the case of a food composition comprising a guarana extract, a vitamin and a natural extract, the present invention was completed by finding that it exhibits a fatigue recovery effect, an antioxidant effect, and a DNA damage protection effect.
따라서, 본 발명의 목적은 과라나 추출물, 비타민 및 천연물 추출물을 유효성분으로 포함하는 식품 조성물을 제공하는 것이다. Accordingly, an object of the present invention is to provide a food composition comprising a guarana extract, a vitamin and a natural product extract as an active ingredient.
본 발명의 일 양태에 따르면, 본 발명은 과라나 추출물, 비타민 및 천연물 추출물을 유효성분으로 포함하는 식품 조성물을 제공한다.According to one aspect of the present invention, the present invention provides a food composition comprising a guarana extract, a vitamin and a natural extract as an active ingredient.
과라나 (Guarana, 학명: Paullinia cupana) 또는 구아라나는 브라질 아마존 분지 원산의 무환자나무과 폴리니아속의 덩굴식물이다. 과라나는 피로회복, 혈액순환, 뇌 활동을 촉진시키는 식물의 열매이며, 커피의 약 3배에 달하는 카페인을 함유하고 있지만 과라나에 함유된 식물성 카페인은 일반 커피나 홍차와는 달리 신경을 자극하지 않는 것이 특징이다. Guarana (scientific name: Paullinia cupana) or Guarana is a vine of the genus Polynia, native to the Amazon Basin of Brazil. Guarana is a fruit of a plant that promotes recovery from fatigue, blood circulation, and brain activity. It contains about three times as much caffeine as coffee. characteristic.
본 명세서에서 사용되는 용어 "추출물"은 당업계에서 조추출물(crude extract)로 통용되는 의미를 갖지만, 광의적으로는 추출물을 추가적으로 분획(fractionation)한 분획물도 포함한다. 즉, 과라나 추출물 또는 천연물 추출물은 추출 용매를 이용하여 얻은 것뿐만 아니라, 여기에 정제과정을 추가적으로 적용하여 얻은 것도 포함한다. 예컨대, 상기 추출물을 일정한 분자량 컷-오프 값을 갖는 한외 여과막을 통과시켜 얻은 분획, 다양한 크로마토그래피 (크기, 전하, 소수성 또는 친화성에 따른 분리를 위해 제작된 것)에 의한 분리 등, 추가적으로 실시된 다양한 정제 방법을 통해 얻어진 분획도 본 발명의 추출물에 포함되는 것이다.As used herein, the term "extract" has the meaning commonly used as a crude extract in the art, but in a broad sense also includes a fraction obtained by additionally fractionating the extract. That is, the guarana extract or natural product extract includes not only those obtained using an extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. The fraction obtained through the purification method is also included in the extract of the present invention.
본 발명의 조성물에서 이용되는 추출물은 과라나에 추출용매를 처리하여 수득하는 경우에는, 다양한 추출용매가 이용될 수 있다. 바람직하게는, 극성용매 또는 비극성 용매를 이용할 수 있다. 극성 용매로서 적합한 것은, (i) 물, (ii) 알코올(바람직하게는, 메탄올, 에탄올, 프로판올, 부탄올, 노말-프로판올, 이소-프로판올, 노말-부탄올), (iii) 아세트산, (iv) DMFO(dimethyl-formamide) 및 (v) DMSO(dimethyl sulfoxide)를 포함한다.When the extract used in the composition of the present invention is obtained by treating Guaranae with an extraction solvent, various extraction solvents may be used. Preferably, a polar solvent or a non-polar solvent may be used. Suitable as polar solvents are: (i) water, (ii) alcohols (preferably methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, n-butanol), (iii) acetic acid, (iv) DMFO (dimethyl-formamide) and (v) DMSO (dimethyl sulfoxide).
비극성 용매로서 적합한 것은, 아세톤, 아세토나이트릴, 에틸 아세테이트, 메틸 아세테이트, 플루오로알칸, 펜탄, 헥산, 2,2,4-트리메틸펜탄, 데칸, 사이클로헥산, 사이클로펜탄, 디이소부틸렌, 1-펜텐, 1-클로로부탄, 1-클로로펜탄, o-자일렌, 디이소프로필 에테르, 2-클로로프로판, 톨루엔, 1-클로로프로판, 클로로벤젠, 벤젠, 디에틸 에테르, 디에틸 설파이드, 클로로포름, 디클로로메탄, 1,2-디클로로에탄, 어닐린, 디에틸아민, 에테르, 사염화탄소 및 THF를 포함한다.Suitable nonpolar solvents are acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, 1- Pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloro methane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride and THF.
본 발명에서 과라나 추출시, 열수 추출법, 냉침 추출법, 환류추출법, 상온 추출법, 또는 초음파 추출법을 사용할 수 있으나 이에 제한되지는 않는다. In the present invention, when extracting guarana, hot water extraction method, cold extraction method, reflux extraction method, room temperature extraction method, or ultrasonic extraction method may be used, but is not limited thereto.
본 발명의 일 구현예에 있어서, 상기 과라나 추출물은 물, 유기용매 및 이들의 혼합 용매로 이루어진 군으로부터 선택되는 어느 하나의 추출 용매로 추출한 것으로서, 상기 유기용매는 알코올, 석유 에테르, 헥산, 벤젠, 클로로포름, 메틸렌클로라이드, 에테르, 에틸아세테이트, 및 아세톤으로 이루어진 군으로부터 선택되는 것이다. In one embodiment of the present invention, the guarana extract is extracted with any one extraction solvent selected from the group consisting of water, an organic solvent, and a mixed solvent thereof, and the organic solvent is alcohol, petroleum ether, hexane, benzene, It is selected from the group consisting of chloroform, methylene chloride, ether, ethyl acetate, and acetone.
본 발명의 일 구체예에 있어서, 상기 알코올은 메탄올, 에탄올, 프로판올, 부탄올, 노말-프로판올, 이소-프로판올, 노말-부탄올 및 이들의 혼합 용매로 이루어진 군으로부터 선택되는 것이다. In one embodiment of the present invention, the alcohol is selected from the group consisting of methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, and a mixed solvent thereof.
본 발명의 일 구체예에 있어서, 상기 에탄올 용액의 농도는 30 내지 100 부피 %이다.In one embodiment of the present invention, the concentration of the ethanol solution is 30 to 100% by volume.
보다 구체적으로 상기 에탄올 용액의 농도는 50 내지 99 부피 %, 60 내지 99 부피 %, 70 내지 99 부피 %, 80 내지 99 부피 %, 90 내지 99 부피 %, 50 내지 98 부피 %, 60 내지 98 부피 %, 70 내지 98 부피 %, 80 내지 98 부피 %, 90 내지 98 부피 %이다. 가장 구체적으로 상기 에탄올 용액의 농도는 95 내지 98 부피 %이다. More specifically, the concentration of the ethanol solution is 50 to 99% by volume, 60 to 99% by volume, 70 to 99% by volume, 80 to 99% by volume, 90 to 99% by volume, 50 to 98% by volume, 60 to 98% by volume , 70 to 98% by volume, 80 to 98% by volume, 90 to 98% by volume. Most specifically, the concentration of the ethanol solution is 95 to 98% by volume.
본 발명에서 이용되는 과라나 추출물은 감압 증류 및 동결 건조 또는 분무 건조 등과 같은 추가적인 과정에 의해 분말 상태로 제조할 수 있다. Guarana extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.
본 명세서에서 사용되는 용어 "천연물 추출물"은 자연계에서 발견되는 살아있는 유기체에 의해 생성되는 화합물 또는 물질로서, 천연물 추출물은 육상식물, 해양생물 또는 미생물의 배양액 등에서 추출될 수 있다. As used herein, the term "natural extract" is a compound or substance produced by a living organism found in nature, and the natural extract may be extracted from a culture medium of land plants, marine organisms, or microorganisms.
본 발명의 식품 조성물에 포함되는 천연물 추출물의 추출 방식에 관한 내용은 상기 과라나 추출물의 추출방식에 관한 내용과 동일하기 때문에, 이 둘 사이에 공통된 내용은 본 명세서의 과도한 복잡성을 피하기 위하여, 그 기재를 생략한다. Since the content regarding the extraction method of the natural extract contained in the food composition of the present invention is the same as the content regarding the extraction method of the guarana extract, the content common between the two is to avoid excessive complexity of the present specification, the description omit
본 명세서에서 사용되는 용어 "유효성분으로 포함하는"이란, 상기 과라나 추출물, 비타민, 천연물 추출물의 효능 또는 활성을 달성하는데 충분한 양을 포함하는 것을 의미한다.As used herein, the term "comprising as an active ingredient" means including an amount sufficient to achieve the efficacy or activity of the guarana extract, vitamins, and natural extracts.
본 발명의 조성물에 포함된 과라나 추출물 및 천연물 추출물은 천연재료로서 세포 독성이 없다. 본 발명의 조성물에 포함된 추출물 및 비타민의 양적 상한은 당업자가 적절한 범위 내에서 선택하여 실시할 수 있다. Guarana extract and natural product extract contained in the composition of the present invention is a natural material and has no cytotoxicity. The upper limit of the quantity of extracts and vitamins contained in the composition of the present invention may be selected by those skilled in the art within an appropriate range.
본 발명의 식품 조성물은 유효성분으로서 상기 추출물 및 비타민뿐 만 아니라, 식품 제조 시에 통상적으로 첨가되는 성분을 포함할 수 있으며, 예를 들어, 단백질, 탄수화물, 지방, 영양소, 조미제 및 향미제를 포함한다. 상술한 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스, 올리고당 등; 및 폴리사카라이드, 예를 들어 덱스트린, 사이클로덱스트린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 향미제로서 천연 향미제 [타우마틴, 스테비아 추출물 (예를 들어 레바우디오시드 A, 글리시르히진 등]) 및 합성 향미제(사카린, 아스파르탐 등)를 사용할 수있다. 예컨대, 본 발명의 식품 조성물이 드링크제로 제조되는 경우에는 본 발명의 과라나 추출물 이외에 구연산, 액상과당, 설탕, 포도당, 초산, 사과산, 과즙 등을 추가로 포함할 수 있다.The food composition of the present invention may include not only the extract and vitamins as active ingredients, but also ingredients commonly added during food production, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. include Examples of the above-mentioned carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatine, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared as a drink, citric acid, high fructose, sugar, glucose, acetic acid, malic acid, fruit juice, etc. may be additionally included in addition to the guarana extract of the present invention.
본 발명의 일 구현예에 있어서, 상기 과라나 추출물, 비타민 및 천연물 추출물은 1:0.1-0.9:0.1-0.9의 중량비로 혼합된다.In one embodiment of the present invention, the guarana extract, vitamins and natural extracts are mixed in a weight ratio of 1:0.1-0.9:0.1-0.9.
보다 구체적으로는 상기 과라나 추출물, 비타민 및 천연물 추출물의 중량비는 1:0.1-0.7:0.1-0.9, 1:0.1-0.5:0.1-0.9, 1:0.1-0.4:0.1-0.9, 1:0.2-0.9:0.1-0.9, 1:0.2-0.7:0.1-0.9, 1:0.2-0.5:0.1-0.9, 1:0.1-0.9:0.2-0.7, 1:0.1-0.9:0.2-0.5, 1:0.1-0.9:0.3-0.7, 1:0.1-0.9:0.3-0.5, 1:0.2-0.7:0.2-0.7, 1:0.2-0.7:0.2-0.5, 1:0.2-0.5:0.2-0.7, 1:0.2-0.5:0.2-0.5, 1:0.2-0.5:0.3-0.7, 1:0.2-0.5:0.3-0.5, 1:0.2-0.5:0.4-0.7, 1:0.2-0.5:0.4-0.6이다. 가장 구체적으로는 1:0.2-0.4:0.4-0.5의 중량비로 혼합된다.More specifically, the weight ratio of the guarana extract, vitamins and natural extracts is 1:0.1-0.7:0.1-0.9, 1:0.1-0.5:0.1-0.9, 1:0.1-0.4:0.1-0.9, 1:0.2-0.9 :0.1-0.9, 1:0.2-0.7:0.1-0.9, 1:0.2-0.5:0.1-0.9, 1:0.1-0.9:0.2-0.7, 1:0.1-0.9:0.2-0.5, 1:0.1-0.9 :0.3-0.7, 1:0.1-0.9:0.3-0.5, 1:0.2-0.7:0.2-0.7, 1:0.2-0.7:0.2-0.5, 1:0.2-0.5:0.2-0.7, 1:0.2-0.5 :0.2-0.5, 1:0.2-0.5:0.3-0.7, 1:0.2-0.5:0.3-0.5, 1:0.2-0.5:0.4-0.7, 1:0.2-0.5:0.4-0.6. Most specifically, it is mixed in a weight ratio of 1:0.2-0.4:0.4-0.5.
본 발명의 일 구현예에 있어서, 상기 비타민은 비타민 A, 비타민 B군, 비타민 C, 비타민 D, 비타민 E, 비타민 K 및 이들의 조합으로 이루어진 군으로부터 선택되는 것이다. In one embodiment of the present invention, the vitamin is selected from the group consisting of vitamin A, vitamin B group, vitamin C, vitamin D, vitamin E, vitamin K, and combinations thereof.
보다 구체적으로, 상기 비타민은 비타민 B군, 비타민 C, 비타민 E 및 이들의 조합으로 이루어진 군으로부터 선택되는 것이다. More specifically, the vitamin is selected from the group consisting of vitamin B group, vitamin C, vitamin E, and combinations thereof.
본 발명의 일 구체예에 있어서, 상기 비타민 B군은 비타민 B1, 비타민 B2, 비타민 B3, 비타민 B4, 비타민 B5, 비타민 B6, 비타민 B7, 비타민 B9, 비타민 B12, 콜린 및 이들의 조합으로 이루어진 군으로부터 선택되는 것이다. In one embodiment of the present invention, the vitamin B group is vitamin B 1 , vitamin B 2 , vitamin B 3 , vitamin B 4 , vitamin B 5 , vitamin B 6 , vitamin B 7 , vitamin B 9 , vitamin B 12 , choline and combinations thereof.
보다 구체적으로, 상기 비타민 B군은 비타민 B1, 비타민 B2, 비타민 B5, 콜린 및 이들의 조합으로 이루어진 군으로부터 선택되는 것이다.More specifically, the vitamin B group is selected from the group consisting of vitamin B 1 , vitamin B 2 , vitamin B 5 , choline, and combinations thereof.
본 발명의 일 구현예에 있어서, 상기 천연물은 오미자, 멜론, 부유, 복숭아, 골드키위, 딸기, 방울토마토, 송본, 참외, 레드키위, 청포도, 태추, 흑토마토, 참다래, 머루열매, 거봉, 사과, 레드글러브, 아오리사과, 배, 귤, 마늘, 적근대, 오이고추, 미나리, 배, 애호박, 오이, 자색감자, 양파, 포도, 시금치, 자색고구마, 당근, 쥬키니호박, 살구, 풋고추, 브로콜리, 새송이버섯, 고구마, 수박, 조선호박 및 이들의 조합으로 이루어진 군으로부터 선택되는 것이다. In one embodiment of the present invention, the natural product is Schisandra, melon, flounder, peach, gold kiwi, strawberry, cherry tomato, songbon, melon, red kiwi, green grape, jujube, black tomato, dandelion fruit, maru fruit, geobong, apple , Red glove, Aori apple, pear, tangerine, garlic, red beet, cucumber pepper, water parsley, pear, zucchini, cucumber, purple potato, onion, grape, spinach, purple sweet potato, carrot, zucchini pumpkin, apricot, green pepper, broccoli, king oyster It is selected from the group consisting of mushrooms, sweet potatoes, watermelons, Korean pumpkins, and combinations thereof.
보다 구체적으로 상기 천연물은 귤, 단감, 딸기, 멜론, 사과, 오미자, 토마토 및 이들의 조합으로 이루어진 군으로부터 선택되는 것이다. More specifically, the natural product is selected from the group consisting of tangerines, sweet persimmons, strawberries, melons, apples, omija, tomatoes, and combinations thereof.
귤은 감귤류에 해당하는 시트러스속 식물이며, 밀감, 한라봉, 천혜향, 청견, 금귤 등이 이에 해당할 수 있다. 귤은 비타민 C와 베타카로틴이 풍부해 면역력을 강화하고, 눈의 건강에 도움을 준다. Tangerine is a plant of the genus citrus that corresponds to the citrus family, and tangerine, hallabong, cheonhyehyang, cheongyeon, kumquat, etc. may correspond to this. Mandarin oranges are rich in vitamin C and beta-carotene, which strengthens immunity and aids in eye health.
단감은(Diospyros kaki)은 비타민 C와 A가 풍부해 특히 일교차가 심한 환절기의 감기 예방에 좋을 뿐만 아니라 전염병 예방과 눈의 생리적 활동에도 유용하게 쓰인다. 또한, 감은 단백질과 지방, 탄수화물, 회분, 인산과 철분 등을 함께 함유하고 있다.Sweet persimmon (Diospyros kaki) is rich in vitamins C and A, so it is not only good for preventing colds, especially during the changing seasons, but also useful for preventing infectious diseases and physiological activities of the eyes. In addition, persimmons contain protein, fat, carbohydrates, ash, phosphoric acid and iron together.
딸기는 장미과에 속하는 다년생 식물로 재배 기간이 길고 노동력이 많이 드는 작물에 속하지만 저온에서 생육이 가능하며 저가온 시설재배로 11월부터 5월까지 수확할 수 있다. 재배딸기에는 플라보노이드(flavonid)류로 퀘르세틴-7-자일로사이드(quercetin-7-xyloside), 프라가린(fragarin), 프로시아니딘 B3(procyanidin B3), 1-O-p-쿠마로일글루코스(1-O-p-coumaroylglucose), 2-헵탄올(2-heptanol) 또는 엘라직산(ellagic acid) 등을 포함하는 것으로 알려져 있다. 이에 딸기를 이용한 항염, 항암, 신장질환 등 질환에 효과가 있다. Strawberry is a perennial plant belonging to the Rosaceae family and belongs to a crop that has a long cultivation period and requires a lot of labor, but can be grown at low temperatures and can be harvested from November to May through low-temperature facility cultivation. Flavonoids in cultivated strawberries include quercetin-7-xyloside, fragarin, procyanidin B3, 1-Op-coumaroylglucose ), 2-heptanol (2-heptanol) or ellagic acid is known to include. Therefore, it is effective in diseases such as anti-inflammatory, anti-cancer, and kidney disease using strawberries.
멜론은 당질의 함량이 높고 철분, 나이아신, 비타민 A, C가 많이 함유가 되어있으며, 혈액의 점도를 낮추어 심장병이나 뇌졸중을 막아주는 항응고제로 효과가 크며, 항암효과가 있는 베타카로틴이 풍부하게 들어 있다.Melon has a high sugar content and contains a lot of iron, niacin, vitamins A and C. It is an anticoagulant that lowers blood viscosity and prevents heart disease or stroke. .
사과는 쌍떡잎식물 장미목 장미과의 낙엽교목의 식물인 사과나무의 열매로서, 유기산과 당류와 같은 기호성 성분 이외에도 식이섬유, 비타민, 미네랄 성분 등을 다량 함유하여 심혈관계 질환 및 암 등 성인병을 예방하는 효과가 있다.Apples are the fruits of the apple tree, a plant of the deciduous tree of the Rosaceae, a dicotyledonous plant, and contain a large amount of dietary fiber, vitamins, and minerals in addition to palatable ingredients such as organic acids and sugars. have.
오미자는 오미자나무의 열매로, 오미자 나무는 지리산, 속리산, 태백산에서 많이 자라는 덩굴성 낙엽 활엽수이다. 오미자는 신맛이 강하고 8~9월에 홍색으로 익으며 길 이는 0.6~1.2 cm이고 여러 개가 포도 송이 모양으로 밑으로 처져 달린다. 오미자는 시잔드린, 고미신, 시트럴, 사과산, 시트르산 등의 성분이 들어 있어 심장을 강하게 하고 혈압을 내리며 면역력을 높여 주어 강장제로 쓴다. 또한, 오미자는 폐 기능을 강하게 하고 진해·거담 작용이 있어서 기침이나 갈증 등을 치료하는 효과가 있다. Omija is the fruit of the Schisandra tree, and the Schisandra tree is a deciduous broad-leaved vine that grows widely in Mt. Jiri, Mt. Songni, and Mt. Taebaek. Omija has a strong sour taste and ripens in red from August to September. Omija contains ingredients such as shizandrine, gomisin, citral, malic acid, and citric acid, and is used as a tonic by strengthening the heart, lowering blood pressure, and increasing immunity. In addition, omija strengthens lung function and has antitussive and expectorant effects, so it is effective in treating cough and thirst.
토마토(Solanum lycopersicum)는 라이코펜(lycopene)이 세포의 산화를 막아 노화를 억제하고, 심혈관 질환과 각 종 암의 예방효과가 있다고 보고되었으며, 비타민 A가 딸기에 비하여 8배 이상 함유되어 있고, 비타민 C는 채소 작물 가운데 가장 많은 양이 함유되어있다. 토마토의 유기산 함량은 citric acid가 0.5 ~ 21%를 차지하며, 유리 아미노산이 0.07 ~ 0.09 % 함유되어 독특한 맛을 주고 노화방지, 퇴행성 질환 억제, 인체면역기능강화의 효능을 가지고 있으며, 루틴(routine) 성분으로 인해 혈관을 튼튼하게 하고 혈압을 내리는 효과가 있다. Tomato (Solanum lycopersicum) has been reported that lycopene inhibits aging by preventing cell oxidation, and has a preventive effect on cardiovascular disease and various cancers. It contains 8 times more vitamin A than strawberries, and vitamin C contains the highest amount among vegetable crops. As for the organic acid content of tomatoes, citric acid accounts for 0.5 to 21%, and free amino acids 0.07 to 0.09% to give a unique taste, prevent aging, suppress degenerative diseases, and strengthen human immune function. Due to its ingredients, it strengthens blood vessels and lowers blood pressure.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 및 귤 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비, 상기 귤 추출물의 중량부는 1 내지 30 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 귤 추출물의 중량부는 3 내지 25, 5 내지 20, 7 내지 15 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 귤 추출물의 중량부는 10 내지 15 중량부이다.In one embodiment of the present invention, the food composition of the present invention is to include a guarana extract, a vitamin and a tangerine extract, compared to 100 parts by weight of the guarana extract, the weight part of the tangerine extract is 1 to 30 parts by weight. More specifically, compared to 100 parts by weight of the guarana extract, the parts by weight of the tangerine extract are 3 to 25, 5 to 20, 7 to 15 parts by weight. Most specifically, compared to 100 parts by weight of the guarana extract, the weight part of the tangerine extract is 10 to 15 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 및 딸기 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비, 상기 딸기 추출물의 중량부는 1 내지 30 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 딸기 추출물의 중량부는 3 내지 25, 5 내지 20, 7 내지 15 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 딸기 추출물의 중량부는 10 내지 15 중량부이다.In one embodiment of the present invention, the food composition of the present invention is to include a guarana extract, a vitamin and a strawberry extract, compared to 100 parts by weight of the guarana extract, the weight part of the strawberry extract is 1 to 30 parts by weight. More specifically, relative to 100 parts by weight of the guarana extract, parts by weight of the strawberry extract are 3 to 25, 5 to 20, and 7 to 15 parts by weight. Most specifically, relative to 100 parts by weight of the guarana extract, the weight part of the strawberry extract is 10 to 15 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 및 오미자 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비, 상기 오미자 추출물의 중량부는 1 내지 30 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 오미자 추출물의 중량부는 3 내지 25, 5 내지 20, 7 내지 15 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 오미자 추출물의 중량부는 7 내지 13 중량부이다. In one embodiment of the present invention, the food composition of the present invention includes a guarana extract, a vitamin, and a Schisandra extract, and 100 parts by weight of the guarana extract, the weight part of the Schisandra extract is 1 to 30 parts by weight. More specifically, compared to 100 parts by weight of the Guarana extract, the weight part of the Schisandra extract is 3 to 25, 5 to 20, 7 to 15 parts by weight. Most specifically, compared to 100 parts by weight of the Guarana extract, the weight part of the Schisandra extract is 7 to 13 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 및 단감 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비, 상기 단감 추출물의 중량부는 1 내지 10 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 단감 추출물의 중량부는 3 내지 9, 4 내지 8 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 단감 추출물의 중량부는 5 내지 7 중량부이다.In one embodiment of the present invention, the food composition of the present invention comprises a guarana extract, a vitamin and a sweet persimmon extract, compared to 100 parts by weight of the guarana extract, the weight part of the sweet persimmon extract is 1 to 10 parts by weight. More specifically, compared to 100 parts by weight of the guarana extract, the weight part of the sweet persimmon extract is 3 to 9, 4 to 8 parts by weight. Most specifically, compared to 100 parts by weight of the guarana extract, the weight part of the sweet persimmon extract is 5 to 7 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 및 토마토 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비, 상기 토마토 추출물의 중량부는 0.5 내지 8 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 토마토 추출물의 중량부는 1 내지 6 중량부, 1.5 내지 4.5 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 토마토 추출물의 중량부는 2 내지 4 중량부이다.In one embodiment of the present invention, the food composition of the present invention is to include a guarana extract, vitamins and tomato extract, compared to 100 parts by weight of the guarana extract, the weight part of the tomato extract is 0.5 to 8 parts by weight. More specifically, compared to 100 parts by weight of the guarana extract, parts by weight of the tomato extract are 1 to 6 parts by weight, 1.5 to 4.5 parts by weight. Most specifically, compared to 100 parts by weight of the guarana extract, parts by weight of the tomato extract are 2 to 4 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 C 및 천연 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비, 상기 비타민 C의 중량부는 0.5 내지 8 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 비타민 C의 중량부는 1 내지 6, 1.5 내지 4.5 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 비타민 C의 중량부는 2 내지 4 중량부이다.In one embodiment of the present invention, the food composition of the present invention is to include a guarana extract, vitamin C and a natural extract, compared to 100 parts by weight of the guarana extract, the weight part of the vitamin C is 0.5 to 8 parts by weight. More specifically, compared to 100 parts by weight of the guarana extract, the weight part of the vitamin C is 1 to 6, 1.5 to 4.5 parts by weight. Most specifically, compared to 100 parts by weight of the guarana extract, the weight part of the vitamin C is 2 to 4 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 E 및 천연 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비, 상기 비타민 E의 중량부는 0.05 내지 0.5 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 비타민 E의 중량부는 0.1 내지 0.3 중량부이다. In one embodiment of the present invention, the food composition of the present invention is to include a guarana extract, vitamin E and a natural extract, compared to 100 parts by weight of the guarana extract, the weight part of the vitamin E is 0.05 to 0.5 parts by weight. More specifically, relative to 100 parts by weight of the guarana extract, the weight part of the vitamin E is 0.1 to 0.3 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 B군 및 천연 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비, 상기 비타민 B군의 중량부는 1 내지 60 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 비타민 B군의 중량부는 5 내지 50, 10 내지 45, 15 내지 40 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 비타민 B군의 중량부는 15 내지 35 중량부이다.In one embodiment of the present invention, the food composition of the present invention is to include guarana extract, vitamin B group and natural extract, compared to 100 parts by weight of the guarana extract, the weight part of the vitamin B group is 1 to 60 parts by weight . More specifically, compared to 100 parts by weight of the guarana extract, the parts by weight of the vitamin B group are 5 to 50, 10 to 45, 15 to 40 parts by weight. Most specifically, compared to 100 parts by weight of the guarana extract, the weight part of the vitamin B group is 15 to 35 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 콜린 및 천연 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비, 상기 콜린의 중량부는 5 내지 50 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 콜린의 중량부는 10 내지 40, 15 내지 35, 15 내지 30 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비, 상기 콜린의 중량부는 18 내지 26 중량부이다.In one embodiment of the present invention, the food composition of the present invention is to include a guarana extract, choline and a natural extract, compared to 100 parts by weight of the guarana extract, the weight part of the choline is 5 to 50 parts by weight. More specifically, compared to 100 parts by weight of the guarana extract, the weight part of the choline is 10 to 40, 15 to 35, 15 to 30 parts by weight. Most specifically, compared to 100 parts by weight of the guarana extract, the weight part of the choline is 18 to 26 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 B1, 비타민 B2, 비타민 B5 및 천연 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비 상기 비타민 B1, 비타민 B2, 비타민 B5 의 중량부는 0.05 내지 1 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비 상기 비타민 B1, 비타민 B2, 비타민 B5 의 중량부는 0.1 내지 0.9, 0.2 내지 0.8, 0.3 내지 0.8 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비 상기 비타민 B1, 비타민 B2, 비타민 B5 의 중량부는 0.4 내지 0.8 중량부이다.In one embodiment of the present invention, the food composition of the present invention is to include a guarana extract, vitamin B 1 , vitamin B 2 , vitamin B 5 and a natural extract, and the vitamin B 1 , vitamins compared to 100 parts by weight of the guarana extract B 2 and vitamin B 5 are in an amount of 0.05 to 1 part by weight. More specifically, the amount of vitamin B 1 , vitamin B 2 , and vitamin B 5 based on 100 parts by weight of the guarana extract is 0.1 to 0.9, 0.2 to 0.8, 0.3 to 0.8 parts by weight. Most specifically, the amount of vitamin B 1 , vitamin B 2 , and vitamin B 5 is 0.4 to 0.8 parts by weight relative to 100 parts by weight of the guarana extract.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 B3 및 천연 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비 상기 비타민 B3의 중량부는 0.1 내지 5 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비 상기 비타민 B3의 중량부는 0.2 내지 4, 0.3 내지 3, 0.4 내지 2 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비 상기 비타민 B3의 중량부는 0.5 내지 1.5 중량부이다.In one embodiment of the present invention, the food composition of the present invention comprises a guarana extract, vitamin B 3 and a natural extract, and the weight part of the vitamin B 3 relative to 100 parts by weight of the guarana extract is 0.1 to 5 parts by weight. More specifically, the amount of vitamin B 3 relative to 100 parts by weight of the guarana extract is 0.2 to 4, 0.3 to 3, 0.4 to 2 parts by weight. Most specifically, the weight part of the vitamin B 3 relative to 100 parts by weight of the guarana extract is 0.5 to 1.5 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 B6 및 천연 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비 상기 비타민 B6의 중량부는 0.01 내지 0.2 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비 상기 비타민 B6의 중량부는 0.03 내지 0.17 중량부이다. 가장 구체적으로 상기 과라나 추출물 100 중량부 대비 상기 비타민 B6의 중량부는 0.05 내지 0.15 중량부이다.In one embodiment of the present invention, the food composition of the present invention comprises a guarana extract, vitamin B 6 and a natural extract, and the weight part of the vitamin B 6 relative to 100 parts by weight of the guarana extract is 0.01 to 0.2 parts by weight. More specifically, the weight part of the vitamin B 6 relative to 100 parts by weight of the guarana extract is 0.03 to 0.17 parts by weight. Most specifically, the weight part of the vitamin B 6 relative to 100 parts by weight of the guarana extract is 0.05 to 0.15 parts by weight.
본 발명의 일 구체예에 있어서, 본 발명의 식품 조성물은 과라나 추출물, 비타민 B12 및 천연 추출물을 포함하는 것이며, 상기 과라나 추출물 100 중량부 대비 상기 비타민 B12의 중량부는 0.0005 내지 0.0015 중량부이다. 보다 구체적으로 상기 과라나 추출물 100 중량부 대비 상기 비타민 B12의 중량부는 0.0007 내지 0.0014 중량부이다. In one embodiment of the present invention, the food composition of the present invention comprises a guarana extract, vitamin B 12 and a natural extract, and the weight part of the vitamin B 12 relative to 100 parts by weight of the guarana extract is 0.0005 to 0.0015 parts by weight. More specifically, the weight part of the vitamin B 12 relative to 100 parts by weight of the guarana extract is 0.0007 to 0.0014 parts by weight.
본 발명의 일 구현예에 있어서, 상기 식품 조성물은 피로회복용 조성물인 것이다. In one embodiment of the present invention, the food composition is a composition for recovery from fatigue.
본 발명의 일 구현예에 있어서, 상기 식품 조성물은 항산화용 조성물인 것이다. In one embodiment of the present invention, the food composition is an antioxidant composition.
본 발명의 일 구현예에 있어서, 상기 식품 조성물은 DNA 손상 보호용 조성물인 것이다. In one embodiment of the present invention, the food composition is a composition for protecting DNA damage.
상기 본 발명의 DNA 손상은 산화적 스트레스에 의해 유발된 것이나, 이에 제한되지 않는다. The DNA damage of the present invention is induced by oxidative stress, but is not limited thereto.
본 발명의 일 구현예에 있어서, 상기 조성물의 형태는 분말, 과립, 정제, 캡슐 및 음료로 이루어진 군으로부터 선택되는 것이다. In one embodiment of the present invention, the form of the composition is selected from the group consisting of powders, granules, tablets, capsules, and beverages.
보다 구체적으로 상기 조성물의 형태는 분말 형태인 것이다. More specifically, the composition is in the form of a powder.
본 발명의 실시예에 따르면 과라나 추출물, 비타민, 천연물 추출물을 포함하는 식품 조성물은 H2O2에 의해 유도된 산화 스트레스에 대한 회복 효과가 있으며, 총 플로보노이드 함량이 높은 것을 확인하였다. 또한 본 발명의 조성물은 DPPH 라디칼 소거능 분석 및 ORAC 활성 분석, CAC 분석을 통해 뛰어난 항산화 효과가 있는 것을 확인하였다. According to an embodiment of the present invention, a food composition comprising a guarana extract, a vitamin, and a natural product extract has a recovery effect on oxidative stress induced by H 2 O 2 , and it was confirmed that the total flavonoid content was high. In addition, it was confirmed that the composition of the present invention has an excellent antioxidant effect through DPPH radical scavenging activity analysis, ORAC activity analysis, and CAC analysis.
본 발명의 실시예에 따르면 본 발명의 상기 조성물은 comet assay를 통해 산화적 손상에 의한 DNA 손상 보호 효과가 있음을 확인하였으며, 설문조사를 통해 피로 회복 효과가 있음을 확인하였다. According to an embodiment of the present invention, it was confirmed that the composition of the present invention had an effect of protecting DNA damage due to oxidative damage through comet assay, and it was confirmed that there was a fatigue recovery effect through a survey.
결론적으로 본 발명의 과라나 추출물, 비타민, 천연물 추출물을 포함하는 식품 조성물은 항산화 효과, DNA 손상 보호 효과, 피로 회복 효과가 있다. In conclusion, the food composition comprising the guarana extract, vitamins, and natural extracts of the present invention has an antioxidant effect, a DNA damage protection effect, and a fatigue recovery effect.
본 발명의 특징 및 이점을 요약하면 다음과 같다:The features and advantages of the present invention are summarized as follows:
본 발명은 과라나 추출물, 비타민 및 천연물 추출물을 유효성분으로 포함하는 식품 조성물을 제공한다. The present invention provides a food composition comprising a guarana extract, a vitamin and a natural extract as an active ingredient.
본 발명의 식품 조성물을 이용하는 경우, 피로 회복 효과, 항산화 효과, DNA 손상 보호 효과를 나타낸다. When the food composition of the present invention is used, it exhibits a fatigue recovery effect, an antioxidant effect, and a DNA damage protection effect.
도 1은 MTT assay를 통해 측정한 에너지 드링크 믹스의 산화 스트레스 회복 효과를 다른 시판제품과 비교한 결과를 나타낸다.
도 2는 Folin-Denis법을 통해 총 폴리페놀 함량을 측정하여 분석한 에너지 드링크 믹스의 항산화 효과를 다른 시판제품과 비교한 결과를 나타낸다.
도 3은 Davis법을 통해 총 플라보노이드 함량을 측정하여 분석한 에너지 드링크 믹스의 항산화 효과를 다른 시판제품과 비교한 결과를 나타낸다.
도 4는 DPPH 라디칼 소거능을 측정하여 분석한 에너지 드링크 믹스의 항산화 효과를 다른 시판제품과 비교한 결과를 나타낸다.
도 5는 ORAC 분석법을 이용하여 분석한 에너지 드링크 믹스의 항산화 효과를 다른 시판제품과 비교한 결과를 나타낸다.
도 6은 CAC(Cellular antioxidant capacity)를 측정하여 분석한 에너지 드링크 믹스의 항산화 효과를 다른 시판제품과 비교한 결과를 나타낸다.
도 7은 Comet assay를 통해 Tail DNA의 함량을 측정하여 분석한 에너지 드링크 믹스의 DNA 손상 보호 효과를 다른 시판제품과 비교한 결과를 나타낸다. 1 shows the results of comparing the oxidative stress recovery effect of an energy drink mix measured by MTT assay with other commercially available products.
Figure 2 shows the results of comparing the antioxidant effect of the energy drink mix analyzed by measuring the total polyphenol content through the Folin-Denis method with other commercially available products.
3 shows the results of comparing the antioxidant effect of the energy drink mix analyzed by measuring the total flavonoid content through the Davis method with other commercially available products.
4 shows the results of comparing the antioxidant effect of the energy drink mix analyzed by measuring the DPPH radical scavenging ability with other commercially available products.
5 shows the results of comparing the antioxidant effect of the energy drink mix analyzed using the ORAC method with other commercially available products.
6 shows the results of comparing the antioxidant effect of the energy drink mix analyzed by measuring CAC (Cellular antioxidant capacity) with other commercially available products.
7 shows the results of comparing the DNA damage protection effect of the energy drink mix analyzed by measuring the content of tail DNA through the Comet assay with other commercially available products.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .
실시예Example
본 명세서 전체에 걸쳐, 특정 물질의 농도를 나타내기 위하여 사용되는 "%"는 별도의 언급이 없는 경우, 고체/고체는 (중량/중량) %, 고체/액체는 (중량/부피) %, 그리고 액체/액체는 (부피/부피) %이다.Throughout this specification, "%" used to indicate the concentration of a specific substance is (weight/weight) % for solid/solid, (weight/volume) % for solid/liquid, and Liquid/liquid is (volume/volume) %.
제조예 1: 에너지 드링크 믹스 제조에 사용되는 과라나 추출물의 제조Preparation Example 1: Preparation of guarana extract used for energy drink mix preparation
브라질산 과라나 열매 추출 분말을 구입하여(MOS Mall, 서울, 한국) 5 ℃ 냉장고에 보관하면서 사용하였다. 추출 분말은 95% 에탄올 추출을 하였으며, 농축은 회전진공농축기(EYELA N-1000, Tokyo Rikakikai Co., Tokyo, Japan)로 37 ℃에서 농축하였다. Brazilian Guarana fruit extract powder was purchased (MOS Mall, Seoul, Korea) and used while stored in a refrigerator at 5 °C. The extracted powder was extracted with 95% ethanol, and the concentration was concentrated at 37°C using a rotary vacuum concentrator (EYELA N-1000, Tokyo Rikakikai Co., Tokyo, Japan).
제조예 2: 에너지 드링크 믹스 제조에 사용되는 천연물 분말의 제조Preparation Example 2: Preparation of natural powder used for energy drink mix preparation
에너지 드링크 믹스 제조에 사용할 천연물은 귤, 단감, 딸기, 멜론, 사과, 오미자, 토마토이다. 상기 천연물을 동결 건조한 후 분쇄기로 분쇄하여 분말화를 진행하였으며, 분쇄한 동결건조 분말을 5 ℃냉장고에 보관하면서 사용하였다. 천연물은 95% 에탄올 추출을 하였으며, 농축은 회전진공농축기(EYELA N-1000, Tokyo Rikakikai Co., Tokyo, Japan)로 37 ℃에서 농축하였다.The natural products to be used in the energy drink mix production are tangerines, sweet persimmons, strawberries, melons, apples, omija, and tomatoes. After freeze-drying the natural product, it was pulverized by pulverizing with a grinder, and the pulverized freeze-dried powder was used while being stored in a refrigerator at 5°C. The natural product was extracted with 95% ethanol, and the concentration was concentrated at 37°C with a rotary vacuum concentrator (EYELA N-1000, Tokyo Rikakikai Co., Tokyo, Japan).
제조예 3: 에너지 드링크 믹스의 제조 Preparation Example 3: Preparation of energy drink mix
에너지 드링크 믹스를 제조하기 위해 모든 재료는 분말화된 것을 사용하였으며, 에너지 드링크 믹스의 주된 재료인 과라나, 비타민, 천연물재료를 표 1에 나타난 조성으로 제조하였다.All materials were powdered to prepare the energy drink mix, and guarana, vitamins, and natural ingredients, which are the main ingredients of the energy drink mix, were prepared in the composition shown in Table 1.
실시예 1: 에너지 드링크 믹스의 산화 스트레스 회복 효과 Example 1: Oxidative stress recovery effect of energy drink mix
마우스기원 근세포(C2C12) 세포주(ATCC, Virginia, USA)를 DMEM 배지 (Sigma Aldrich, Missouri, USA)에 10% 소태아 혈청(fetal bovine serum, FBS), 1% 글루코스(glucose), 1% 글루타민(glutamine), 100 U/mL 페니실린(penicillin), 100 μg/mL 스트렙토마이신(streptomycin)을 포함한 배양액으로 95 %의 습도가 유지되는 37 ℃, 5% CO2의 배양기(incubator)에서 배양하였다.Mouse-origin myocyte (C2C12) cell line (ATCC, Virginia, USA) was added to DMEM medium (Sigma Aldrich, Missouri, USA) with 10% fetal bovine serum (FBS), 1% glucose, 1% glutamine ( glutamine), 100 U/mL penicillin, and 100 μg/mL streptomycin in a culture medium containing 95% humidity at 37 °C, 5% CO 2 Incubated in an incubator.
C2C12 세포를 실험 전날 1×105 cells/mL 농도로 96-웰 플레이트(well plate)에 시딩하고, 시료와 1 mM H2O2를 처리하여 24시간 동안 배양하였다. 그 후 4 ℃에 보관 중인 MTT를 5 mg/mL의 농도로 PBS에 녹인 다음 주사기를 이용하여 첨가하고, 피펫을 이용하여 96-웰 미디움(well medium) 부피의 20 % 되는 양 만큼 취하여 각각의 웰에 조심스럽게 첨가하여 플레이트를 바닥에 붙인 상태로 전후좌우로 가볍게 흔들면서 혼합하였다. CO2 배양기에서 4시간 동안 배양한 후 배양액을 제거하고, DMSO 100 μL를 첨가하여 포르마잔(formazan)을 용출시킨 뒤 CO2 배양기에서 30분 동안 배양하였다. 30분 후 540 nm 흡광도에서 측정하여 세포 생존능을 계산하였다.C2C12 cells were seeded in a 96-well plate at a concentration of 1×10 5 cells/mL the day before the experiment , treated with the sample and 1 mM H 2 O 2 , and incubated for 24 hours. Thereafter, MTT stored at 4°C was dissolved in PBS at a concentration of 5 mg/mL and then added using a syringe, and an amount equal to 20% of the volume of 96-well medium was taken using a pipette to each well. was carefully added to the mixture, and the plate was attached to the floor by gently shaking back and forth and left and right. After culturing for 4 hours in a CO 2 incubator, the culture solution was removed, and formazan was eluted by adding 100 μL of DMSO, followed by incubation in a CO 2 incubator for 30 minutes. Cell viability was calculated by measuring at 540 nm absorbance after 30 min.
산화 스트레스 회복 효과를 측정하기 위해 실시한 MTT reduction 분석 결과, 제조예 3의 에너지 드링크 믹스의 세포생존율(26.3 ± 1.3 %)은 1 mM H2O2로 산화적 스트레스를 유도한 PC (양성대조군)보다 유의적으로 23.5%만큼 높았다 (표 2, 도 1). As a result of the MTT reduction analysis performed to measure the oxidative stress recovery effect, the cell viability (26.3 ± 1.3%) of the energy drink mix of Preparation Example 3 was higher than that of PC (positive control group) induced by oxidative stress with 1 mM H 2 O 2 significantly as high as 23.5% (Table 2, Figure 1).
생존율cell
survival rate
NC: 정상대조군, PC: 1 mM H2O2로 산화적 스트레스를 유도한 양성대조군, A: 시판제품 A, B: 시판제품 B, C: 제조예 3의 에너지 드링크 믹스NC: normal control, PC: positive control induced by oxidative stress with 1 mM H 2 O 2 A: Commercial products A, B: Commercial products B, C: Energy drink mix of Preparation Example 3
a-c; 각 그룹별 유의성을 표시함(p<0.05)a-c; Significance for each group was indicated (p<0.05)
실시예 2: 에너지 드링크 믹스의 항산화 효과 분석Example 2: Analysis of antioxidant effect of energy drink mix
실시예 2-1. 에너지 드링크 믹스의 총 폴리페놀(polyphenol) 함량 분석Example 2-1. Analysis of total polyphenol content in energy drink mixes
총 폴리페놀 함량은 Folin-Denis법에 따라 비색 정량하였다. 시료 1 mL에 증류수 1 mL를 가하여 희석하고 1 N Folin-Ciocalteu's phenol reagent 2 mL을 가하여 실온에서 3분간 방치한 후, 10% Na2CO3용액 2 mL을 가하여 이 혼합액을 1시간 동안 상온에서 방치하였다. 1시간 후 혼합액을 13,400 g에서 5분간 원심분리하고 상층액 200 μL를 취하여 ELISA 리더(Sunrise, Tecan Co. Ltd., Grφdig, Austria)를 사용하여 690 nm에서 흡광도를 측정하였다. 표준물질로 갈릭산(gallic acid)을 사용하여 검량선을 작성하고, 총 페놀 함량은 mg GAE/100 g (mg garlic acid equivalents/100 g) 단위로 나타내었다.The total polyphenol content was colorimetrically quantified according to the Folin-Denis method. Add 1 mL of distilled water to 1 mL of sample to dilute, add 2 mL of 1 N Folin-Ciocalteu's phenol reagent, leave at room temperature for 3 minutes, add 2 mL of 10% Na 2 CO 3 solution, and leave the mixture at room temperature for 1 hour. did. After 1 hour, the mixture was centrifuged at 13,400 g for 5 minutes, 200 μL of the supernatant was taken, and absorbance was measured at 690 nm using an ELISA reader (Sunrise, Tecan Co. Ltd., Grφdig, Austria). A calibration curve was prepared using gallic acid as a standard material, and the total phenol content was expressed in mg GAE/100 g (mg garlic acid equivalents/100 g).
제조예 3의 에너지 드링크 믹스의 총 폴리페놀 함량은 300.5 ± 1.3 mg GAE/100 g 으로 나타났으며, 시판제품 A와 시판제품 B보다 유의적으로 낮았다 (표 3, 도 2).The total polyphenol content of the energy drink mix of Preparation Example 3 was 300.5 ± 1.3 mg GAE/100 g, which was significantly lower than that of commercial products A and B (Table 3, FIG. 2 ).
A: 시판제품 A, B: 시판제품 B, C: 제조예 3의 에너지 드링크 믹스A: Commercial products A, B: Commercial products B, C: Energy drink mix of Preparation Example 3
a-c; 각 그룹별 유의성을 표시함(p<0.05)a-c; Significance for each group was indicated (p<0.05)
실시예 2-2. 에너지 드링크 믹스의 총 플라보노이드(flavonoid) 함량의 분석Example 2-2. Analysis of total flavonoid content of energy drink mixes
총 플라보노이드 함량은 Davis법에 따라 비색 정량하였다. 시료 0.1 mL에 디에틸 글리콜(diethyl glycol) 1 mL을 가하여 실온에서 5분간 방치한 후, 1 N NaOH 용액 0.1 mL을 가하여 이 혼합액을 30분 동안 상온에서 방치하였다. 반응이 끝난 혼합액은 UV/VIS spectrometer-(UV 1601, shimadzu, Kyoto, Japan)를 사용하여 420 nm에서 흡광도를 측정하였다. 표준물질로 퀘르세틴(quercetin)을 사용하여 검량선을 작성하고, 총 플라보노이드 함량은 mg QE/100 g (mg quercetin equivalents/100 g) 단위로 나타내었다. Total flavonoid content was colorimetrically quantified according to the Davis method. 1 mL of diethyl glycol was added to 0.1 mL of the sample, left at room temperature for 5 minutes, 0.1 mL of 1 N NaOH solution was added, and the mixture was left at room temperature for 30 minutes. The absorbance of the reaction mixture was measured at 420 nm using a UV/VIS spectrometer-(UV 1601, shimadzu, Kyoto, Japan). A calibration curve was prepared using quercetin as a standard material, and the total flavonoid content was expressed in mg QE/100 g (mg quercetin equivalents/100 g).
제조예 3의 에너지 드링크 믹스의 총 플라보노이드 함량은 1076.8 ± 0.0 mg QE/100 g 으로 시판제품 A (474.2 ± 1.6 QE/100 g)와 시판제품 B (298.8 ± 0.4 QE/100 g) 보다 각각 127.1 %, 260.4 %만큼 유의적으로 높았다 (표 4, 도 3). The total flavonoid content of the energy drink mix of Preparation Example 3 was 1076.8 ± 0.0 mg QE/100 g, which was 127.1% of the commercial product A (474.2 ± 1.6 QE/100 g) and the commercial product B (298.8 ± 0.4 QE/100 g), respectively. , was significantly higher by 260.4% (Table 4, Fig. 3).
A: 시판제품 A, B: 시판제품 B, C: 제조예 3의 에너지 드링크 믹스 A: Commercial products A, B: Commercial products B, C: Energy drink mix of Preparation Example 3
a-c; 각 그룹별 유의성을 표시함(p<0.05)a-c; Significance for each group was indicated (p<0.05)
실시예 2-3. 에너지 드링크 믹스의 DPPH 라디칼 소거능 분석Example 2-3. Analysis of DPPH radical scavenging activity of energy drink mix
DPPH 라디칼 소거능은 Mensor 등의 방법을 변형하여 측정하였다. 시료 20 μL에 0.4 mM DPPH 에탄올 용액 80 μL을 가하여 10초 동안 잘 혼합한 후 상온에서 10분간 반응시키고, 반응이 끝난 혼합액은 ELISA 리더(Sunrise, Tecan Co. Ltd., Grφdig, Austria)를 이용하여 492 nm에서 흡광도를 측정하였다. DPPH radical scavenging ability was measured by modifying the method of Mensor et al. Add 80 μL of 0.4 mM DPPH ethanol solution to 20 μL of the sample, mix well for 10 seconds, and then react at room temperature for 10 minutes. Absorbance was measured at 492 nm.
제조예 3의 에너지 드링크 믹스의 DPPH 라디칼 소거능은 65.7 ± 0.7 %로 시판제품 B(64.2 ± 0.6 %)와 동일한 활성을 나타내었으며, 시판제품 A (25.1 ± 3.3 %) 보다 161.8 %만큼 유의적으로 높았다 (표 5, 도 4). The DPPH radical scavenging ability of the energy drink mix of Preparation Example 3 was 65.7 ± 0.7%, indicating the same activity as that of commercial product B (64.2 ± 0.6%), and was significantly higher than that of commercial product A (25.1 ± 3.3%) by 161.8%. (Table 5, Figure 4).
A: 시판제품 A, B: 시판제품 B, C: 제조예 3의 에너지 드링크 믹스A: Commercial products A, B: Commercial products B, C: Energy drink mix of Preparation Example 3
a-b; 각 그룹별 유의성을 표시함(p<0.05)a-b; Significance for each group was indicated (p<0.05)
실시예 2-4. 에너지 드링크 믹스의 산소 라디칼 흡수 용량(Oxygen radical absorbance capacity, ORAC) 활성 분석Example 2-4. Oxygen radical absorbance capacity (ORAC) activity analysis of energy drink mixes
퍼옥실 라디칼(Peroxyl radical) 소거능을 통한 항산화 활성 측정은 퍼옥실 라디칼 소거능(peroxyl radical scavenging capacity, ORACROO) 분석법을 이용하였다. 시료 50 μL에 버퍼 100 μL, 퍼옥실 라디칼 생성을 위한 80 nM AAPH 50 μL 및 80 nM 플루오레스세인(fluorescein) 50 μL를 가한 후 GENios 형광판 리더(fluorescence plate reader, Tecan Trading AG, Salzburg, Austria)를 이용하여 여기 파장(excitation wavelength) 485 nm, 방사 파장(emission wavelength) 535 nm에서 매 2 분마다 2시간 동안 측정하였다. 최종 반응 농도 1 μM의 트롤록스(trolox)를 표준물질로 사용하고, ORAC 값은 각 시료의 형광이 감소하는 곡선 아래 부분의 총 면적(net area under the curve)을 산출하여 1 μM 트롤록스 등가물(trolox equivalents, TE)로 나타내었다. Antioxidant activity was measured through peroxyl radical scavenging ability using peroxyl radical scavenging capacity (ORAC ROO ) analysis. 100 μL of buffer, 50 μL of 80 nM AAPH for generation of peroxyl radicals, and 50 μL of 80 nM fluorescein were added to 50 μL of sample, followed by a GENios fluorescence plate reader (Tecan Trading AG, Salzburg, Austria). It was measured for 2 hours every 2 minutes at an excitation wavelength of 485 nm and an emission wavelength of 535 nm using the The final reaction concentration of 1 μM trolox was used as a standard, and the ORAC value was calculated as the net area under the curve in which the fluorescence of each sample decreased, and 1 μM Trolox equivalent ( trolox equivalents, TE).
제조예 3의 에너지 드링크 믹스의 ORAC 활성은 50 μg/mL의 농도에서 3.86 ± 0.05 1 μM TE로 시판제품 A(3.51 ± 0.28 %)와 유의적인 차이는 없었으나 10.0 %만큼 높았으며, 시판제품 B(3.02 ± 0.29 %)보다 27.8 %만큼 유의적으로 높았다 (표 6, 도 5). The ORAC activity of the energy drink mix of Preparation Example 3 was 3.86 ± 0.05 1 µM TE at a concentration of 50 µg/mL, and there was no significant difference from the commercial product A (3.51 ± 0.28 %), but was as high as 10.0 %, and the commercial product B (3.02 ± 0.29%) by 27.8% (Table 6, Fig. 5).
A: 시판제품 A, B: 시판제품 B, C: 제조예 3의 에너지 드링크 믹스A: Commercial products A, B: Commercial products B, C: Energy drink mix of Preparation Example 3
a-b; 각 그룹별 유의성을 표시함(p<0.05)a-b; Significance for each group was indicated (p<0.05)
실시예 2-5. 에너지 드링크 믹스의 세포 항산화 능력(Cellular antioxidant capacity, CAC) 분석Example 2-5. Cellular antioxidant capacity (CAC) analysis of energy drink mixes
인체기원 간세포(HepG2) 세포주(KCLB, 서울, 한국)를 DMEM 배지(Sigma Aldrich, Missouri, USA)에 10% 소태아혈청(FBS), 1% 글루코스, 1% 글루타민, 100 U/mL 페니실린, 100 μg/mL 스트렙토마이신을 포함한 배양액으로 95%의 습도가 유지되는 37 ℃, 5% CO2의 배양기에서 배양하였다. Human-origin hepatocyte (HepG2) cell line (KCLB, Seoul, Korea) was added to DMEM medium (Sigma Aldrich, Missouri, USA) with 10% fetal bovine serum (FBS), 1% glucose, 1% glutamine, 100 U/mL penicillin, 100 It was cultured in a culture medium containing μg/mL streptomycin at 37° C., maintained at 95% humidity, in an incubator of 5% CO 2 .
HepG2 세포를 5×105 cells/mL로 계산하여 96-웰 플레이트(Greiner, 655090)에 100 μL씩 가하여 37 ℃, 5% CO2의 배양기에서 배양하였다. 24시간 후 배지를 제거하고 형광에 안정한 HBSS(Hank's balanced salt solution)를 각 웰에 200 μL 분주한 후 최종 농도가 100 μg/mL이 되도록 각 시료 2 μL를 처리하여 30분 동안 배양하였다. HBSS 50 μL로 각 웰을 세척하고 다시 HBSS 200 μL 분주한 후 퍼옥실 라디칼 발생기(peroxyl radical generator)로 8 mM AAPH 2 μL를 가하여 30 분 동안 배양하였다. 이 때 AAPH는 매번 신선하게 제조하여 사용하였다. 형광 프로브(probe)로 40 mM DCFH-DA 2 μL를 가한 후 빛을 차단하여 30 분 동안 배양한 후 FLUOstar OPTIMA 마이크로 플레이트 리더(BMG Lab Technology; 485 nm 여기 파장 및 538 nm 방사 파장)를 이용하여 형광의 발생 정도를 측정하였다. 대조군은 DCFH-DA만을 처리하였으며, AAPH와 DCFH-DA를 처리하여 양성 대조군으로 하였다. 대조군의 형광값을 100 %로 하여 각 시료와 양성 대조군인 AAPH의 상대적인 형광 값을 비교하였다.HepG2 cells were counted at 5×10 5 cells/mL, and 100 μL of each was added to a 96-well plate (Greiner, 655090) and cultured in an incubator at 37° C., 5% CO 2 . After 24 hours, the medium was removed, 200 µL of fluorescence-stable Hank's balanced salt solution (HBSS) was dispensed into each well, and 2 µL of each sample was treated to a final concentration of 100 µg/mL and incubated for 30 minutes. Each well was washed with 50 μL of HBSS, then 200 μL of HBSS was again dispensed, and 2 μL of 8 mM AAPH was added with a peroxyl radical generator and incubated for 30 minutes. At this time, AAPH was prepared fresh each time and used. After adding 2 μL of 40 mM DCFH-DA as a fluorescent probe, blocking the light and incubating for 30 minutes, fluorescence using a FLUOstar OPTIMA microplate reader (BMG Lab Technology; 485 nm excitation wavelength and 538 nm emission wavelength) was measured. As a control group, only DCFH-DA was treated, and AAPH and DCFH-DA were treated as a positive control. The relative fluorescence values of each sample and the positive control, AAPH, were compared by setting the fluorescence value of the control to 100%.
모든 제품의 CAC 활성은 PC (양성대조군)와 비교하여 유의적으로 감소하였으며, 에너지 드링크 믹스의 CAC 활성은 127.2 ± 1.4 %로 PC보다 70.1% 감소하여 AAPH 라디칼으로부터 세포를 보호하는 높은 효과를 보였다. 또한 에너지 드링크 믹스의 CAC 활성은 시판제품 A 및 시판제품 B보다 각각 25.8 %, 16.6 %만큼 높았다 (표 7, 도 6). The CAC activity of all products was significantly reduced compared to PC (positive control), and the CAC activity of the energy drink mix was 127.2 ± 1.4%, which was 70.1% lower than that of PC, showing a high effect of protecting cells from AAPH radicals. In addition, the CAC activity of the energy drink mix was 25.8% and 16.6% higher than those of commercial product A and commercial product B, respectively (Table 7, FIG. 6).
NC: 정상대조군, PC: AAPH로 산화적 스트레스를 유도한 양성대조군, A: 시판제품 A, B: 시판제품 B, C: 제조예 3의 에너지 드링크 믹스NC: normal control group, PC: positive control group induced by oxidative stress with AAPH, A: commercial product A, B: commercial product B, C: energy drink mix of Preparation Example 3
a-d; 각 그룹별 유의성을 표시함(p<0.05)a-d; Significance for each group was indicated (p<0.05)
실시예 3: 에너지 드링크 믹스의 DNA 손상 보호 효과 분석Example 3: Analysis of DNA damage protection effect of energy drink mix
인체기원 간세포(HepG2) 세포주를 DMEM 배지에 10% 소태아 혈청(FBS), 1 % 글루코스, 1 % 글루타민, 100 U/mL 페니실린, 100 μg/mL 스트렙토마이신을 포함한 배양액으로 95 %의 습도가 유지되는 37 ℃, 5% CO2 배양기에서 배양하였다.Human-derived hepatocyte (HepG2) cell line is cultured in DMEM medium containing 10% fetal bovine serum (FBS), 1% glucose, 1% glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin, maintaining 95% humidity. 37 ° C., 5% CO 2 Incubated in an incubator.
분리한 간세포에 시료를 농도별(1, 5, 10, 50 μg/mL)로 처리하여 37 ℃에서 30분간 반응시키고, 반응이 끝난 후 간세포를 PBS로 세척한 후 인위적인 산화적 스트레스를 유발하기 위하여 200 μM의 H2O2를 4 ℃에서 5분간 처리한 후 PBS로 세척하였다. 500 g에서 5분간 원심분리한 후 얻어진 세포를 0.7% 저융점 아가로즈(low melting agarose, LMA)에 골고루 섞고 1% 정상 융점 아가로즈(normal melting agarose, NMA)가 프리코팅된 슬라이드에 놓고 커버글라스를 덮어 골고루 분산시킨 후 4 ℃에 20분간 방치하였다. 겔이 굳으면 커버글라스를 벗기고 다시 0.7% LMA 용액 75 μL로 중층하여 4 ℃에 20분간 방치하였다. 겔이 굳으면 차가운 알칼리 용해 버퍼(2.5M NaCl, 100 mM Na2EDTA, 10 mM Tris, 1% sodium lauryl sarcosine, 1% Triton X-100, 10% DMSO)에 슬라이드를 담가 4 ℃에서 암실조건으로 1시간 동안 침지시켜 DNA 이중가닥을 풀어주었음. 용해가 끝난 후, 슬라이드를 4 ℃의 전기영동 버퍼 (300 mM NaOH, 10 mM Na2EDTA, pH>13)에 20분간 방치하여 DNA의 알칼리 불안정 위치(alkali labile sites)가 드러나도록 풀어준 후, 25 V/300±3 mA의 전압으로 20분간 전기영동을 실시하였다. 전기영동이 끝난 후 슬라이드를 차가운 중성용액(0.4 M Tris buffer, pH 7.4)에 5분간 3회, 에탄올에 5분간 1회 세척한 후 건조하였다. 슬라이드를 브로민화 에티듐(20 μg/mL)으로 염색하고, DNA 손상도는 형광현미경(LEICA DMLB, Wetzlar, Germany)의 CCD camera(Nikon, Tokyo, Japan)를 통해 보내진 세포핵 이미지를 comet image analyzing system(Komet version 5.0, Kinetic Imaging, Liverpool, UK)을 이용하여 분석하였다.In order to induce artificial oxidative stress after treating the separated hepatocytes with samples by concentration (1, 5, 10, 50 μg/mL) and reacting at 37 °C for 30 minutes, after the reaction is completed, hepatocytes are washed with PBS. 200 μM of H 2 O 2 was treated at 4° C. for 5 minutes, and then washed with PBS. After centrifugation at 500 g for 5 minutes, the obtained cells are mixed evenly in 0.7% low melting agarose (LMA), and then placed on a slide precoated with 1% normal melting agarose (NMA) and placed on a cover glass. Covered and evenly dispersed, then left at 4 °C for 20 minutes. When the gel hardened, the cover glass was removed, layered again with 75 µL of 0.7% LMA solution, and left at 4 °C for 20 minutes. When the gel hardens, immerse the slides in cold alkaline lysis buffer (2.5M NaCl, 100 mM Na 2 EDTA, 10 mM Tris, 1% sodium lauryl sarcosine, 1% Triton X-100, 10% DMSO) in the dark at 4 °C. DNA double strands were released by immersion for 1 hour. After dissolution, the slides were left in an electrophoresis buffer (300 mM NaOH, 10 mM Na 2 EDTA, pH>13) at 4 ° C for 20 minutes to release alkali labile sites of DNA. Electrophoresis was performed for 20 minutes at a voltage of 25 V/300±3 mA. After electrophoresis, the slides were washed three times for 5 minutes in cold neutral solution (0.4 M Tris buffer, pH 7.4) and once in ethanol for 5 minutes, and then dried. The slides were stained with ethidium bromide (20 μg/mL), and the DNA damage level was measured using a CCD camera (Nikon, Tokyo, Japan) of a fluorescence microscope (LEICA DMLB, Wetzlar, Germany). (Komet version 5.0, Kinetic Imaging, Liverpool, UK) was used for analysis.
DNA 손상 보호 효과를 측정하기 위해 실시한 comet assay 결과, 제조예 3의 에너지 드링크 믹스는 PC (양성대조군)보다 Tail DNA (%)가 75.2 %만큼 유의적으로 감소하였다. 또한, 제조예 3의 에너지 드링크 믹스는 Tail DNA (%)가 시판제품 A보다 46.0 %만큼, 시판제품 B보다 29.4 %만큼 감소하는 효과를 보였다. As a result of the comet assay performed to measure the DNA damage protection effect, the energy drink mix of Preparation Example 3 significantly reduced tail DNA (%) by 75.2% compared to PC (positive control). In addition, the energy drink mix of Preparation Example 3 showed the effect of reducing the tail DNA (%) by 46.0% than the commercial product A and by 29.4% than the commercial product B.
NC: 정상대조군, PC: 1 mM H2O2로 산화적 스트레스를 유도한 양성대조군, A: 시판제품 A, B: 시판제품 B, C: 제조예 3의 에너지 드링크 믹스NC: normal control, PC: positive control induced by oxidative stress with 1 mM H 2 O 2 A: Commercial products A, B: Commercial products B, C: Energy drink mix of Preparation Example 3
a-c; 각 그룹별 유의성을 표시함(p<0.05)a-c; Significance for each group was indicated (p<0.05)
실시예 4: 설문조사를 통한 에너지 드링크 믹스의 항피로 효과 분석Example 4: Analysis of anti-fatigue effect of energy drink mix through survey
연구 수행 6시간 전(14:00)부터 카페인이 함유된 모든 음료 및 식품의 섭취를 금하였고, 18:00에 피실험자에게 동일한 저녁식사를 제공하였다. 에너지 드링크 믹스 및 위약 드링크 믹스 섭취 시간은 22:00이였다. All beverages and foods containing caffeine were prohibited from 6 hours before the study (14:00), and the same dinner was provided to the subjects at 18:00. The intake time of the energy drink mix and the placebo drink mix was 22:00.
피실험자는 에너지 드링크 믹스와 위약 드링크 믹스 섭취 전(21:30~21:50, 1회), 섭취 직후(22:00) 및 섭취 후 1시간 간격으로 다음날 오전 4:00까지 총 6회 '피로도 항목 설문지(1967년 <일본산업위생협회 산업피로위원회>가 제안하고, 1970년 동 협회에서 확정한 피로자각 증상표(일본노동과학연구소 발행, 1970))'를 작성하였다. 오전 4:00 이후 각자 휴식을 취하고 오전 7:00에 '피로도 항목 설문지'와 '에너지 드링크 믹스 섭취 효과 항목 설문지'를 작성하였다. Subjects were asked to 'fatigue items' 6 times before (21:30~21:50, once), immediately after ingestion (22:00), and at 4:00 a.m. the next day after ingestion of the energy drink mix and placebo drink mix at 1 hour intervals. A questionnaire (a list of symptoms of fatigue perception suggested by the Industrial Fatigue Committee of the Japan Industrial Hygiene Association in 1967 and confirmed by the association in 1970 (published by the Japan Labor Science Research Institute, 1970)) was prepared. After 4:00 am, each took a break and completed the 'Fatigue Questionnaire' and 'Effect of Energy Drink Mix Intake Questionnaire' at 7:00 am.
연구수행시간인 오전 4:00 이전에 5분 이하의 졸음이 발생한 경우에는 연구를 계속 진행하도록 하며, 오전 4:00 이전에 수면을 취한 경우에는 오전 7:00에 수면 시간을 기록하고, '피로도 항목 설문지'와 '에너지 드링크 믹스 섭취 효과 항목 설문지'를 작성하였다. 일주일의 휴약기간을 거친 후 에너지 드링크 믹스 또는 위약 드링크 믹스를 교차 섭취하여 위와 동일한 실험을 실행하였다.If drowsiness of 5 minutes or less occurred before 4:00 am, the study time, the study was continued. 'Item Questionnaire' and 'Energy Drink Mix Intake Effect Item Questionnaire' were completed. After a week-long drug-free period, the same experiment as above was performed by cross-ingesting an energy drink mix or a placebo drink mix.
피로도 자각증상은 신체적 증상, 정신적 증상, 신경감각적 증상으로 구분하였으며, 증상에 따라 각각의 항목은 10개 문항으로 구성하였다. 증상이 있으면 0점, 증상이 없으면 1점을 부여하여 피로도가 최고일 때 0점, 최저일 때 10점으로 나타내었다.The subjective symptoms of fatigue were divided into physical symptoms, mental symptoms, and neurosensory symptoms, and each item consisted of 10 items according to the symptoms. 0 points were given if symptoms were present and 1 point was given if there were no symptoms.
에너지 드링크 믹스 섭취 후 피로도 자각 증상에 대한 설문조사를 실시한 결과, 신체적 증상에서는 에너지 드링크 믹스 섭취 1시간 후(9.00 ± 1.41), 2시간 후(8.33 ± 2.55), 5시간 후(8.33 ± 2.55), 9시간 후(8.22 ± 1.79)에 위약군보다 유의적으로 높은 항피로 효과를 나타내었다. 신경감각적 증상에서도 에너지 드링크 믹스 섭취 1시간 후(10.00 ± 0.00), 2시간 후(10.00 ± 0.00), 3시간 후(9.78 ± 0.67)에 위약군보다 유의적으로 높은 항피로 효과를 나타내었다(표 9). As a result of conducting a survey on the symptoms of fatigue after consuming an energy drink mix, the physical symptoms showed that 1 hour (9.00 ± 1.41), 2 hours (8.33 ± 2.55), 5 hours (8.33 ± 2.55), and 5 hours (8.33 ± 2.55), After 9 hours (8.22 ± 1.79), the anti-fatigue effect was significantly higher than that of the placebo group. In neurosensory symptoms, the anti-fatigue effect was significantly higher than that of the placebo group at 1 hour (10.00 ± 0.00), 2 hours (10.00 ± 0.00), and 3 hours (9.78 ± 0.67) after ingestion of the energy drink mix (Table 9). ).
가장 효과가 높은 섭취 1시간 후 및 섭취 2시간 후의 에너지 드링크 믹스군의 피로도 분석결과를 7점 척도로 나타내었을 때 신체적 증상항목에서 섭취 1시간 후 1.5점, 섭취 2시간 후 2.3점이었으며, 신경감각적 증상항목에서 섭취 1시간 후 1점, 섭취 2시간 후 1점을 나타내었다.When the fatigue analysis result of the energy drink mix group 1 hour after ingestion and 2 hours after ingestion, which had the highest effect, was expressed on a 7-point scale, in the physical symptom category, 1.5 points after 1 hour of ingestion and 2.3 points after 2 hours after ingestion were obtained, and In the symptom item, 1 point after 1 hour of ingestion and 1 point after 2 hours of ingestion were indicated.
*; 각 그룹의 시간대별 유의성을 표시함(p<0.05)*; Indicate the significance of each group by time period (p<0.05)
Claims (11)
A food composition comprising guarana extract, vitamins and natural extracts as active ingredients.
The food composition according to claim 1, wherein the guarana extract, vitamins and natural extract are mixed in a weight ratio of 1:0.1-0.9:0.1-0.9.
The method according to claim 1, wherein the guarana extract and the natural extract are extracted with any one extraction solvent selected from the group consisting of water, an organic solvent, and a mixed solvent thereof, and the organic solvent is alcohol, petroleum ether, hexane, benzene. , chloroform, methylene chloride, ether, ethyl acetate, and will be selected from the group consisting of acetone, the food composition.
The food composition according to claim 1, wherein the alcohol is selected from the group consisting of methanol, ethanol, propanol, butanol, n-propanol, iso-propanol, n-butanol, and mixed solvents thereof.
The food composition of claim 4, wherein the ethanol is an ethanol solution of 50 to 99% by volume.
The food composition according to claim 1, wherein the vitamin is selected from the group consisting of vitamin B group, vitamin C, vitamin E, and combinations thereof.
The food composition according to claim 1, wherein the natural product is selected from the group consisting of tangerines, sweet persimmons, strawberries, melons, apples, omija, tomatoes, and combinations thereof.
The food composition according to claim 1, wherein the food composition is a composition for recovery from fatigue.
The food composition according to claim 1, wherein the food composition is an antioxidant composition.
The food composition according to claim 1, wherein the food composition is a composition for protecting DNA damage.
The food composition according to claim 1, wherein the form of the composition is selected from the group consisting of powders, granules, tablets, capsules and beverages.
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