KR20210094704A - Active Molecular Chitosan Raw Material and Manufacturing Method Thereof - Google Patents
Active Molecular Chitosan Raw Material and Manufacturing Method Thereof Download PDFInfo
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- KR20210094704A KR20210094704A KR1020200008219A KR20200008219A KR20210094704A KR 20210094704 A KR20210094704 A KR 20210094704A KR 1020200008219 A KR1020200008219 A KR 1020200008219A KR 20200008219 A KR20200008219 A KR 20200008219A KR 20210094704 A KR20210094704 A KR 20210094704A
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- 229920001661 Chitosan Polymers 0.000 title claims abstract description 28
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 7
- 239000002994 raw material Substances 0.000 title 1
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 15
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 14
- 230000006196 deacetylation Effects 0.000 claims abstract description 7
- 238000003381 deacetylation reaction Methods 0.000 claims abstract description 7
- 229920002101 Chitin Polymers 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000004042 decolorization Methods 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 239000003963 antioxidant agent Substances 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000005115 demineralization Methods 0.000 description 2
- 230000002328 demineralizing effect Effects 0.000 description 2
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 2
- 241000238017 Astacoidea Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000003544 deproteinization Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/51—Polysaccharide
- A23V2250/511—Chitin, chitosan
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Sustainable Development (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
본 발명은 항산화 활성 및 항균 활성이 증진된 키토산의 제조 방법에 관한 것으로, 더욱 상세하게는 건조된 게The present invention relates to a method for producing chitosan with enhanced antioxidant activity and antibacterial activity, and more particularly, dried crab
껍질을 분쇄한 후 산을 처리하여 탈미네랄화 (demineralization)하는 단계; 상기 탈미네랄화된 게 껍질 분말에Demineralization (demineralization) by treating the acid after pulverizing the peel; In the demineralized crab shell powder
염기를 처리하고 고압 멸균 (autoclaving)하여 단백질 제거 (deproteinization)한 후 키틴을 수득하는 단계; 및Obtaining chitin after protein removal (deproteinization) by treating the base and autoclaving; and
상기 수득된 키틴에 염기를 처리하고 고압 멸균하여 탈아세틸화 (deacetylation)한 후 탈색 (decoloration)하는The obtained chitin is treated with a base and subjected to high pressure sterilization to deacetylation and then decoloration.
단계를 포함하여 제조하는 것을 특징으로 하는 항산화 활성 및 항균 활성이 증진된 키토산의 제조 방법, 상기Method for producing chitosan with enhanced antioxidant activity and antibacterial activity, characterized in that it comprises the steps of:
방법에 의해 제조된 항산화 활성 및 항균 활성이 증진된 키토산 및 상기 항산화 활성 및 항균 활성이 증진된 키Chitosan with enhanced antioxidant activity and antibacterial activity prepared by the method, and a key with enhanced antioxidant activity and antibacterial activity
토산을 함유하는 가공식품에 관한 것이다.It relates to processed foods containing local acids.
키토산 (Chitosan)은 게, 새우 및 가재와 같은 갑각류 껍질의 주요 성분인 키틴[폴리β-(1→4)-N-아세틸-D-글루Chitosan is a major component of the shells of crustaceans such as crab, shrimp and crayfish, chitin [polyβ-(1→4)-N-acetyl-D-glue).
코사민]을 탈아세틸화하여 얻어지는 천연 무독성 바이오폴리머이다. 지난 수십 년간 키토산은 항미생물성, 항종Cosamine] is a natural non-toxic biopolymer obtained by deacetylation. Over the past few decades, chitosan has been shown to have antimicrobial and antimicrobial properties.
양성, 항산화성 및 혈중콜레스테롤 저하 작용과 같은 다양한 생물학적 활성으로 인해 많은 주목을 받아 왔다.It has received much attention due to its various biological activities such as benign, antioxidant and blood cholesterol lowering action.
키토산은 또한 수분과 지방 흡수, 유화작용 및 색소 결합 등의 특성을 지닌 것으로 보고되고 있다 (Li 및 Xia,Chitosan is also reported to have properties such as water and fat absorption, emulsification and pigment binding (Li and Xia,
International Journal of Biological Macromolecules, 48, 768-772, 2011).International Journal of Biological Macromolecules, 48, 768-772, 2011).
본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 분쇄된 게 껍질로부터 제조된 키토산 (g-키토산) 및 온전The present invention has been derived from the above needs, and chitosan (g-chitosan) prepared from pulverized crab shells and whole
한 게 껍질로부터 제조된 키토산 (e-키토산)의 이화학적 및 기능적 특성이 다르게 나타나는 것을 회분 함량, 탈The physicochemical and functional properties of chitosan (e-chitosan) prepared from crab shells are different.
아세틸화도 (deacetylation degree), 용해도 및 점도 등을 분석하여 확인하였고, g-키토산의 항산화 활성 및 항It was confirmed by analyzing deacetylation degree, solubility and viscosity, etc., and the antioxidant activity and antibacterial activity of g-chitosan
균 활성이 e-키토산보다 우수하게 나타나는 것을 확인함으로써, 본 발명을 완성하였다.By confirming that the bacterial activity appears superior to e-chitosan, the present invention was completed.
상기 수득된 키틴에 염기를 처리하고 고압 멸균하여 탈아세틸화 (deacetylation)한 후, 건조하여 탈색The obtained chitin is treated with a base and subjected to high pressure sterilization to deacetylation, followed by drying and decolorization.
(decoloration)하는 단계를 포함하여 제조하는 것을 특징으로 하는 항산화 활성 및 항균 활성이 증진된 키토산Chitosan with enhanced antioxidant activity and antibacterial activity, characterized in that it is prepared including the step of (decoloration)
의 제조 방법을 제공한다.It provides a manufacturing method of
본 발명의 분쇄된 게 껍질로부터 제조된 키토산 (g-키토산)은 온전한 게 껍질로부터 제조된 키토산에 비해 회분Chitosan (g-chitosan) prepared from crushed crab shells of the present invention is ash compared to chitosan prepared from intact crab shells.
함량은 낮은 반면, 탈아세틸화도 (deacetylation degree), 용해도 및 점도가 높고, 항산화 활성 및 항균 활성이While the content is low, deacetylation degree, solubility and viscosity are high, and antioxidant and antibacterial activity are high.
뛰어나기 때문에 항균 조성물 또는 식품 등의 가공소재로 적당한 키토산을 제공할 수 있는 효과가 있다.Because it is excellent, there is an effect of providing suitable chitosan as an antibacterial composition or processing material such as food.
건조된 게 껍질을 분쇄하는 단계Crushing dried crab shells
본 발명의 일 구현 예에 따른 항산화 활성 및 항균 활성이 증진된 키토산 제조 방법에서, 상기 항산화 활성 및In the method for producing chitosan with enhanced antioxidant activity and antibacterial activity according to an embodiment of the present invention, the antioxidant activity and
항균 활성이 증진된 키토산은 온전한 게 껍질로부터 제조된 키토산에 비해 항산화 활성 및 항균 활성이 증진된Chitosan with enhanced antibacterial activity has improved antioxidant and antibacterial activity compared to chitosan prepared from intact crab shells.
것을 의미한다.means that
DPPH (2,2-디페닐-1-피크릴하이드라질)DPPH (2,2-diphenyl-1-picrylhydrazyl)
Claims (1)
(decoloration)하는 단계를 포함하여 제조하는 것을 특징으로 하는 항산화 활성 및 항균 활성이 증진된 키토산
의 제조 방법.The obtained chitin is treated with a base and subjected to high-pressure sterilization to deacetylation, followed by drying and decolorization.
Chitosan with enhanced antioxidant activity and antibacterial activity, characterized in that it is prepared including the step of (decoloration)
manufacturing method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200008219A KR20210094704A (en) | 2020-01-22 | 2020-01-22 | Active Molecular Chitosan Raw Material and Manufacturing Method Thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200008219A KR20210094704A (en) | 2020-01-22 | 2020-01-22 | Active Molecular Chitosan Raw Material and Manufacturing Method Thereof |
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| Publication Number | Publication Date |
|---|---|
| KR20210094704A true KR20210094704A (en) | 2021-07-30 |
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| KR1020200008219A KR20210094704A (en) | 2020-01-22 | 2020-01-22 | Active Molecular Chitosan Raw Material and Manufacturing Method Thereof |
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| KR (1) | KR20210094704A (en) |
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2020
- 2020-01-22 KR KR1020200008219A patent/KR20210094704A/en unknown
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