KR20210094353A - Adenovirus vector - Google Patents

Abstract

The present invention relates to an adenoviral vector, in which a specific partial base in an E2B region is deleted, resulting in large foreign gene loading capacity, low probability of generating replication competent adenovirus, and excellent genetic stability.

Description

Adenovirus vector {ADENOVIRUS VECTOR}

The present invention relates to adenoviral vectors.

Human adenovirus is an icosahedral particle without an outer membrane with a size of 60 to 90 nM. To date, more than 60 serotypes have been identified, which are subdivided into 7 subgroups (A, B, C, D, E, F and G) based on hemagglutination properties and sequence homology. The genome (genome) has a length of 34 to 36 kb and is lateralized at both sites by reverse terminal repeats (ITRs). The viral infection cycle is divided into early and late stages, and the cycle progresses with the expression of specific genes according to each period.

With respect to these early gene regions, adenoviral vectors in which some of these regions have been deleted are known. Adenoviral vectors are widely used as gene delivery systems because of their high efficiency of gene delivery into various cells, extensive clinical experience, and easy production. The types include first-generation adenoviruses in which E1 and E3 were removed among viral genes, second-generation adenoviruses in which E2A or E2B genes were removed in addition to E1 and E3, and helper-dependent adenovirus vectors in which the maximum number of viral genes were removed while leaving only ITR. etc.

Limitation of loading of vector DNA in the case of loading several genes simultaneously on the delivery system to increase the efficacy of gene therapy or loading the non-coding region of DNA on the delivery vehicle to increase the expression strength of a promoter or use it as an antigen If there is not enough, it may not be possible to insert the desired DNA part. The maximum size of a gene that can be loaded into a gene delivery system is determined by the size of the genome, which is a unique characteristic of a virus, and how many virus genes are removed when producing the delivery system. In the case of the second-generation adenovirus vector, more E2B genes are removed, so that a vector with a larger capacity for loading foreign genes than a general E1/E3 removal vector can be constructed. However, theoretically, an intact adenovirus vector cannot be obtained by unconditionally removing the non-functional DNA from the E2B gene. there is.

Korean Patent No. 2039631

An object of the present invention is to provide an adenoviral vector having a large capacity for loading a foreign gene and a low risk of generating a replication-competent adenovirus (Replication-Competent Adenovirus).

An object of the present invention is to provide an adenoviral vector with excellent genetic stability and an increased capacity for loading foreign genes.

1. An adenovirus vector in which bases at positions corresponding to positions 7275 to 7881 and 8583 to 9630 in the E2B region sequence of SEQ ID NO: 1 are deleted.

2. The adenoviral vector according to 1, wherein the deletion causes inactivation of a DNA polymerase gene (pol) and a preterminal protein gene (pTP).

3. The adenoviral vector according to 1 above, wherein the adenoviral vector is an adenoviral vector in which bases at positions corresponding to positions 7274 to 7881 and 8583 to 9630 in the sequence of SEQ ID NO: 1 are deleted.

4. The adenoviral vector according to 1 above, wherein the adenoviral vector has a nucleotide deletion at the position corresponding to positions 7275 to 9632 in the sequence of SEQ ID NO: 1.

5. The adenoviral vector according to 1 above, wherein a foreign gene is introduced into at least a part of the deletion site.

6. A method for producing an adenoviral vector, comprising the step of infecting a packaging cell line with the adenoviral vector of any one of 1 to 5 above.

7. The packaging cell line of 6 above, HEK-293 (Human embryonic kidney 293), HEK-293-derived cells, 911, PER.C6, A549 (carcinomic human alveolar basal epithelial cell), HER (Human Embryonic Retinoblast) , HEL 299 (Human lung fibroblast), HeLa (Human cervical cancer cell), CHO (Chinese hamster ovary cell), BHK (Baby hamster kidney cell), 3T3 (Mouse embryonic fibroblast cell), WS1 (Human dermal fibroblast), MRC5 ( Any one selected from the group consisting of Human Lung Fibroblast), WI38 (Human lung fibroblast cell), AE1-2a (Human lung adenocarcinoma), PC12 (Rat Pheochromocytoma cell), Primary human amniocytes, African green monkey kidney cells, and Cocker Spaniel kidney cells One, a method for producing an adenoviral vector.

8. The method of 7, wherein the packaging cell line expresses a DNA polymerase gene (pol) and a preterminal protein gene (pTP).

9. A cell transformed with the adenoviral vector of any one of 1 to 5 above.

The adenoviral vector of the present invention has an increased gene loading space compared to the prior art, and can also carry large foreign genes.

The adenoviral vector of the present invention has excellent genetic stability.

The adenoviral vector of the present invention has a very low probability of generating a replication competent adenovirus during production.

1 is a restriction enzyme mapping result of adenovirus plasmid DNA having the E2B structure of Preparation Examples 1 to 5 and not loaded with a foreign gene (transgene).
2 is a restriction enzyme mapping result of the adenovirus plasmid DNA having the E2B structure of Preparation Examples 1 to 5 and loaded with a foreign gene.
3 and 4 are results of analyzing the expression pattern of the late adenovirus protein after infecting the packaging cell line (293 cells and 293 cells expressing the E2B gene) with an adenovirus vector.
5 is a PCR result of the E2B region of DNA extracted from each vector not loaded with a foreign gene.
6 is a result of PCR analysis of the E2B region to obtain DNA after one passage culture of the adenovirus vector having the E2B structure of Preparation Example 2 and not loaded with a foreign gene.
7 shows the results of PCR analysis of the E2B region by obtaining DNA from each vector loaded with a foreign gene (HER2).
8 is a sequencing result of the PCR product of the E2B region observed during analysis of a vector having the E2B structure of Preparation Example 1 and loaded with a foreign gene.
9 is a result of restriction enzyme mapping obtained by obtaining DNA from a vector loaded with a foreign gene while having the E2B structure of Preparation Example 1.
10 is a result of restriction enzyme mapping obtained by obtaining DNA from a vector loaded with a foreign gene while having the E2B structure of Preparation Examples 3-5.
11 is a band photograph after purification of a vector loaded with a foreign gene while having the E2B structure of Preparation Examples 3-5.
12 shows the results of PCR analysis of the E2B region and the foreign gene region by obtaining DNA from a purified adenovirus vector loaded with a foreign gene while having the E2B structure of Preparation Examples 3-5.

Hereinafter, the present invention will be described in detail.

The present invention relates to adenoviral vectors. Adenovirus vector is also called recombinant adenovirus vector, and generally refers to an adenovirus derived from a virus existing in nature, but from which some genes have been removed depending on the purpose. In the case of adenoviral vectors, the E1 gene essential for replication is generally removed in order for the virus to replicate only in specific cases, and additional viral gene parts may be removed.

In the adenoviral vector of the present invention, bases at positions corresponding to positions 7275 to 7881 and 8583 to 9630 in the sequence of SEQ ID NO: 1 are deleted.

The sequence of SEQ ID NO: 1 is the Human adenovirus 5 complete genome (NCBI Reference Sequence: AY339865, 35934bp), among which the bases correspond to a part of the E2B region (the portion including the sequence of the pTP gene and the sequence of the Pol gene). , Specifically, bases 7275 to 7881 are a part of a DNA polymerase gene (DNA polymerase gene, pol, SEQ ID NO: 2) in the E2B region, and bases 8583 to 9630 are preterminal protein gene (pTP, sequence It is part of number 3). The gene plays an essential role in the replication of viral DNA, and as the bases are deleted, the function of the E2B gene is inactivated in the adenoviral vector of the present invention to lose the ability to replicate viral DNA.

In the adenoviral vector of the present invention, since the specific site is deleted, the function of E2B is completely blocked, so the possibility of RCA generation is low, and the genetic stability is excellent, and since the deleted site is very large, the size of the gene in the vector is large , it can be used for a variety of purposes without limitation, such as containing genes of several species or containing DNA that does not encode a protein depending on the purpose.

The adenoviral vector of the present invention is one in which the bases at positions corresponding to positions 7275 to 7881 and 8583 to 9630 in the sequence of SEQ ID NO: 1 are deleted, and preferably SEQ ID NO: In the sequence of 1, the base at the position corresponding to positions 7275 to 9632 may be deleted.

The corresponding position may mean a corresponding position when the sequences are aligned. In addition to the sequence of SEQ ID NO: 1, there may be DNA of an adenovirus or adenovirus vector having a different sequence. In the case of aligning the adenovirus sequence of another sequence with the sequence of SEQ ID NO: 1, the position corresponding to the sequence of SEQ ID NO: 1 is selected. Able to know. That is, in the adenoviral vector according to the present invention, the base at the position corresponding to the position of SEQ ID NO: 1 is deleted in the adenoviral vector of another sequence as well as the deletion of the base at the position in the sequence of SEQ ID NO: 1 can

In addition, the corresponding position may be identified based on the degree of similarity of neighboring sequences without aligning the sequences. For example, the adenoviral DNA sequence of AC_000008 (SEQ ID NO: 34), AY601635 (SEQ ID NO: 35), and KX868466 (SEQ ID NO: 36) is based on SEQ ID NO: 1 on both sides of the gene deletion region 30bp of the sequence and at least 90% Matches can be found. For example, bases corresponding to the (7245-7274) and (9633-9662) regions are located on both sides of the region in which bases 7275 to 9632 of SEQ ID NO: 1 are deleted. Sequences that are more than 90% identical to this base can be found at (7236-7265) and (9621-9650) of the KX868466 sequence. Based on this, by deleting (7266-9620) of the KX868466 sequence, an adenoviral vector in which the bases at positions corresponding to positions 7275 to 9632 in the sequence of SEQ ID NO: 1 are deleted can be prepared. In addition to the AC_000008 or AY601635 sequence, an adenovirus vector in which the bases at positions corresponding to positions 7275 to 9632 in the sequence of SEQ ID NO: 1 are deleted can be prepared in the same manner as above.

In addition, in the adenoviral vector of the present invention, the bases at positions corresponding to positions 7274 to 7881 and 8583 to 9630 in the sequence of SEQ ID NO: 1 are deleted, or the bases at positions corresponding to positions 7274 to 9632 are deleted. or the bases at positions corresponding to positions 7274 to 7881 and 8583 to 9632 are deleted, or the bases at positions corresponding to positions 7275 to 7881 and 8583 to 9630 are deleted, or 7275 to The bases at positions corresponding to positions 7881 and 8583 to 9632 may be deleted, or the bases at positions corresponding to positions 7274 to 9630 may be deleted.

When adenovirus DNA is manipulated into a vector, a plasmid containing viral DNA is used to facilitate genetic manipulation. For example, if it is desired to construct a vector in which a specific part of the E2B region of a virus is removed, the corresponding part is removed from the plasmid DNA containing the viral DNA using genetic recombination technology. The adenoviral vector of the present invention was produced using the pAdEasy-1 plasmid of Agilent used in the Examples, but it can also be produced based on the adenovirus plasmid included in other kits. For example, the adenovirus vector of the present invention can also be prepared using the plasmid DNA of the adenovirus included in the Adenovirus Expression Vector Kit (Takara), AdenoZAP Kit (OD 260), ViraPower Adenoviral Gateway Expression Kit (Thermo), etc. using the cosmid method. can be produced

In the adenoviral vector of the present invention, the function of the E2B gene is completely blocked, so the possibility of RCA generation is low. In the case of a general adenoviral vector, the E1 gene, which plays an essential role in the expression of other viral genes, is removed, so virus replication does not occur when cells are infected. However, it is known that some replication-competent adenoviruses (RCA) in which the E1 gene is mounted in the genome of a vector are generated as a result of genetic recombination during production. The adenoviral vector of the present invention does not replicate in the body because there is no E2B gene even when E1 is loaded in the vector genome. If both pTP and Pol genes are loaded in the genome of the adenoviral vector of the present invention, in addition to E1, replication in the body may be possible in theory. .

In addition, it is known that, in general E1-removed adenovirus vectors, a phenomenon called leaky gene expression occurs in which viral DNA replication occurs despite the absence of the E1 gene in some cells infected with the vector and, as a result, other viral genes are expressed. However, in the adenovirus vector of the present invention, the gene directly involved in DNA replication is inactivated, so leaky gene expression does not occur.

The adenoviral vector of the present invention has a large number of bases deleted as described above, can carry a larger foreign gene, and has excellent genetic stability at the deletion site. The genetic stability of a vector can be determined by evaluating whether the DNA obtained from the vector has the same structure as the plasmid DNA. Mainly, the modified part of the viral DNA is amplified by PCR to check the size and sequence of the PCR product, and after treatment with restriction enzymes, the overall structure of the viral DNA is checked by comparing it with the expected result.

In addition to the above, the adenoviral vector of the present invention may be one in which at least a portion of the E1 region, the E3 region, or the E4 region is further deleted. The deletion region and length may be appropriately selected so that there is no problem in productivity, genetic stability, etc. while the function of the gene in the region is lost. Or, it may be included in that area. For example, the E2B removal method of the present invention may be applied to a vector in which regions E1, E3, and E4 are not removed. Depending on the region included, the vector may have various characteristics, and since the E2B region is commonly removed, it has a characteristic that cannot be replicated in cells other than the packaging cell line.

The adenoviral vector of the present invention may include a promoter for gene expression. As used herein, the term “promoter” refers to a DNA sequence that regulates the expression of another DNA sequence operably linked to in a suitable host cell, and “operably linked” as used herein refers to a DNA sequence to perform a general function. It refers to a functional linkage between an expression control sequence and a DNA sequence encoding a target protein or RNA. For example, a promoter and a DNA sequence encoding a protein or RNA may be operably linked to affect expression of the coding sequence. The operative linkage of the promoter in the DNA construct or vector can be prepared using genetic recombination techniques well known in the art, and site-specific DNA cleavage and ligation using enzymes generally known in the art. . In the case of a prokaryotic cell as a host, a strong promoter capable of propagating transcription (eg, pLλ promoter, trp promoter, lac promoter, tac promoter, T5 promoter, T7 promoter, PBAD promoter, etc.), for initiation of translation It is common to include a ribosome binding site and a transcription/translation termination sequence. In the case of a eukaryotic cell as a host, the origin of replication contained in the adenovirus plasmid includes the f1 origin of replication, the SV40 origin of replication, the pMB1 origin of replication, the adeno origin of replication, the AAV origin of replication, the CMV origin of replication, and the BBV origin of replication. The present invention is not limited thereto. In addition, a promoter derived from the genome of a mammalian cell (eg, a metallotionine promoter) or a promoter derived from a mammalian virus (eg, adenovirus late promoter, vaccinia virus 7.5K promoter, SV40 promoter, The cytomegalovirus (CMV) promoter and the tk promoter of HSV) can be used and generally have a polyadenylation sequence as a transcription termination sequence.

The plasmid DNA used to construct the adenoviral vector of the present invention may further include an antibiotic resistance region or an origin of replication.

The adenoviral vector of the present invention may be one in which a foreign gene is introduced into the base deletion site.

As used herein, the term "foreign gene" may include genes of a different origin from the adenovirus of the present invention, genes heterologous to the adenovirus of the present invention, and genes heterogeneous to the host when introduced into an appropriate host.

Examples of the foreign gene include DNA encoding a protein, DNA encoding antisense RNA, and DNA encoding a ribozyme. The origin of such exogenous genes is not particularly limited, and examples thereof include microorganisms such as bacteria, yeasts, radiation fungi, filamentous fungi, ascomycetes, and basidiomycetes; plant; insect; Animals, and the like, and may include genes artificially synthesized according to the purpose, but is not limited thereto. It may also contain portions of DNA that do not encode proteins for the purpose of regulating gene expression or for use as antigens.

The adenoviral vector of the present invention can be produced using a packaging cell line capable of vector replication.

As packaging cell lines, HEK-293 (Human embryonic kidney 293), HEK-293-derived cells, 911, PER.C6 ^TM , A549 (carcinomic human alveolar basal epithelial cell), HER (Human Embryonic Retinoblast), known in the art, HEL 299 (Human lung fibroblast), HeLa (Human cervical cancer cell), CHO (Chinese hamster ovary cell), BHK (Baby hamster kidney cell), 3T3 (Mouse embryonic fibroblast cell), WS1 (Human dermal fibroblast), MRC5 (Human Lung Fibroblast), WI38 (Human lung fibroblast cell), AE1-2a (Human lung adenocarcinoma), PC12 (Rat Pheochromocytoma cell), Primary human amniocytes, African green monkey kidney cells, or cocker spaniel kidney cells, etc. It is not limited. The HEK-293-derived cells may be GP2-293 or 293 T cells, but is not limited thereto.

The packaging cell line may be mutated to express the gene of the deleted region in the adenoviral vector.

A method of transducing the gene into a cell can be performed through various methods known in the art. For example, microinjection (Capecchi, MR, Cell, 22:479 (1980); and Harland and Weintraub, J. Cell Biol. 101:1094-1099 (1985)), calcium phosphate precipitation (Graham, FL et al.) ., Virology, 52:456 (1973); and Chen and Okayama, Mol. Cell. Biol. 7:2745-2752 (1987)), electroporation (Neumann, E. et al., EMBO J., 1: 841 (1982); and Tur-Kaspa et al., Mol. Cell Biol., 6:716-718 (1986)), liposome-mediated transfection (Wong, TK et al., Gene, 10:87 (1980)) ); Nicolau and Sene, Biochim. Biophys. Acta, 721:185-190 (1982); and Nicolau et al., Methods Enzymol., 149:157-176 (1987)), DEAE-dextran treatment (Gopal, Mol); Cell Biol., 5:1188-1190 (1985)), and Gene Bambadment (Yang et al., Proc. Natl. Acad. Sci., 87:9568-9572 (1990)). Cells can be transformed to express

The present invention also relates to a method for producing the adenoviral vector described above.

The method of the present invention cuts the plasmid DNA containing the DNA of the adenoviral vector described above with a restriction enzyme such as PacI to obtain a linear viral DNA with an exposed ITR (Inverted Terminal Repeat) portion, and introduces it into a packaging cell line may include the step of As a method for introducing restriction enzyme-treated DNA, the method exemplified above may be used. This process is called rescue, and a viral vector can be produced from the introduced linear viral DNA. Since the viral vector produced once is capable of self-replication in the packaging cell line, the amount gradually increases if cell culture is continued.

As the packaging cell line, those exemplified above may be used.

As described above, the adenoviral vector is inactivated due to the loss of the functions of the DNA polymerase gene (pol, SEQ ID NO: 2) and preterminal protein gene (pTP, SEQ ID NO: 3). , the vector can be cloned by infecting a packaging cell line expressing the two genes.

When the adenoviral vector further deletes at least a portion of the E1 region, the E3 region, or the E4 region in addition to the above, the packaging cell line may further express the gene of the corresponding region.

The packaging cell line may express the genes endogenously or by introduction of a foreign gene.

When the DNA containing the information of the adenoviral vector is introduced into the packaging cell line expressing the genes, the packaging cell line can produce the adenoviral vector.

As the introduction method, the method exemplified above may be used, but is not limited thereto.

Furthermore, the present invention relates to an adenoviral vector produced from the above-mentioned production method.

When DNA encoding the above-described adenoviral vector is introduced into a packaging cell line, the packaging cell line produces an adenoviral vector, which can be isolated and obtained.

The above-described adenovirus vector is a replication-incompetent virus, and may have an adenovirus-derived structure (particularly, E2B portion) in the same structure as that prepared on plasmid DNA without structural abnormality.

The present invention also relates to a cell transformed with the adenoviral vector.

The adenovirus vector may be one in which a foreign gene is introduced into the aforementioned deleted region, and the adenovirus is produced by transforming it into a packaging cell, and may have the foreign gene. Accordingly, the cells transformed with the adenovirus can express the foreign gene.

As the host cell, any type of cell to be transformed can be applied without limitation.

Transformation may be carried out by a method known in the art, may be by the introduction method exemplified above, but is not limited thereto.

Hereinafter, examples will be given to describe the present invention in detail.

Example

1. Preparation of Adenovirus Plasmids

(1) Preparation Example 1

All adenoviral plasmids used for the adenoviral vector construction of this patent were used for AdEasy ^TM XL Adenoviral vector system (Agilent, Cat#240010), which is a commercial adenovirus plasmid construction system. In this system, a plasmid called pAdEasy-1 (SEQ ID NO: 37) containing the adenovirus genome from which E1 has been removed and a plasmid called pShuttle that can be inserted into the E1 part (SEQ ID NO: 38) and the E1 part are inserted into the E1 part to express foreign genes. It consists of a plasmid called pShuttle-CMV (SEQ ID NO: 39) loaded with a promoter capable of In this patent, after loading the partially cut E2B sequence on the pShuttle plasmid, homologous recombination between pAdEasy-1 and the modified shuttle plasmid was induced to create a plasmid in which the E2B portion was removed based on pAdEasy-1. The plasmid to be introduced into the cell during the production of adenovirus vectors later is not a shuttle plasmid, but a pAdEasy-1 based plasmid, and this plasmid is specifically called an adenovirus plasmid. The adenovirus plasmid used in the vector construction of different preparation examples is prepared from each shuttle plasmid loaded with the E2B sequence, and the procedure for inserting the shuttle plasmid into pAdEasy-1 is well presented in the manual of the ^{AdEasy TM XL system, and briefly} The explanation is as follows.

After cutting the shuttle plasmid with PmeI, heat treatment was performed to completely remove the PmeI enzyme activity (65° C., 20 minutes), or CIP (Alkaline Phosphatase, Calf Intestinal) was treated to prevent reattachment of the portion cut with PmeI. BJ5183-AD1, an E.coli containing pAdEasy-1, was transformed with 1 μg of DNA from the obtained reaction solution itself or from the reaction solution purified by phenol/chloroform method by heat shock method (42℃, 90 seconds). . After confirming the structure by cutting the candidate clone with an enzyme suitable for the structure of each preparation example, only the appropriate clone was transferred to XL-10G, amplified, and the structure was further confirmed. Restriction enzymes frequently used for structural identification include PacI, BstXI and DraI. In order to obtain the final DNA, NucleoBond Xtra Midi Plasmid DNA purification kit (MN, Cat# 740410.100) was used.

The vector of Preparation Example 1 is a vector in which (7186..7891) of the pol gene and (9198.. 9639) of the pTP gene are removed. In Preparation Examples, the position to be removed is described based on the position in the sequence of SEQ ID NO: 1, and since the pAdEasy-1 vector has the E1 region removed, if it is based on the pAdEasy-1 vector, the position is as much as the length of the E1 region. will be pulled forward For example, in the case of the (7186..7891) and (9198..9639) parts, the (7367..8072) and (9379..9820) parts are obtained based on the pAdEasy-1 vector.

First, [5'-CCC GTA TAC AGA CTT GAG AGG CCT GTC CTC GAG C-3', SEQ ID NO: 4] and [5'-GCC GTT TAA ACA] using pAxCAwtit2 (Takara), another plasmid including an adenovirus genome, as a template TCG ATA CCG TTC GGA GGC CGA CG-3', SEQ ID NO: 5]. SEQ ID NO: 4 includes a BstZ17I restriction enzyme site (GTATAC) for cloning immediately before the viral complementary sequence, and CCC is attached immediately before it. In SEQ ID NO: 5, a ClaI restriction enzyme site (ATCGAT) and a PmeI restriction enzyme site (GTTTAAAC) for cloning are attached immediately before the sequence complementary to the virus, and GCC is added in front of it. The PCR product was cloned into pGEM®-T Easy (Promega). The PCR amplification product thus obtained corresponds to (5764-7185) of SEQ ID NO: 1 and is referred to as the A region for convenience.

Thereafter, pAxCAwtit2 (Takara) was added to [5'-GCA TTC GAA ACT CCG CCG CCG AGG GAC CTG-3', SEQ ID NO: 6] and [5'-GCA CAT ATG GTT TAA ACG AAA AGC AAA AAA GGG GC-3', SEQ ID NO: 7] by PCR. In SEQ ID NO: 6, there is a BstBI restriction enzyme site (TTCGAA) used for cloning immediately in front of the viral complementary sequence, and GCA is additionally attached immediately in front of it. In SEQ ID NO: 7, there is a PmeI restriction enzyme site (GTTTAAAC) used for cloning immediately in front of the virus complementary sequence, an NdeI restriction enzyme site (CATATG) is immediately in front of it, and GCA is additionally attached in front of it. The PCR amplification product thus obtained corresponds to (9646-11045) of SEQ ID NO: 1 and is referred to as the B region for convenience. The obtained PCR product was cloned into pGEM®-T Easy.

The B region-loaded plasmid was cut with BstBI and PmeI to obtain an insert, and the A region-loaded plasmid was cut with ClaI and PmeI and cloned to prepare a plasmid loaded with both the A and B regions in the T-vector. As a result of combining A and B, the part corresponding to (7186-9645) between them is deleted. Cut the plasmid containing both A and B with BstZ17I and PmeI to obtain the AB region, and cut ^{the pShuttle plasmid included in the AdEasy TM} XL Adenoviral vector system with BstXI and PmeI in the same way, and then ligate the two to induce a deletion in the E2B part shuttle plasmid. was produced. This process was performed using Takara's In-Fusion® HD Cloning Kit, and the following primers were used: [5'-GTC CGT GTC CCC GTA TAC AGA CTT GAG AGG CCT GTC CT -3', SEQ ID NO: 8] and [5′-CTA TTG AAT TCG TTT AAA CGA AAA GCA AAA AAG GGG C-3′, SEQ ID NO: 9].

The shuttle plasmid prepared in this way can induce a deletion in the E2B portion during homologous recombination with pAdEasy-1, but i-Leader (7952-8391 of SEQ ID NO: 1) and adenovirus RNA, a functional portion other than the E2B portion, The portion involved in the splicing (9640-9645 of SEQ ID NO: 1) is deleted. [5'- GCG GCG GAG TAT CGA TAC CGT TCG GAG GC -3', SEQ ID NO: 10] and [5'- GCC TCC GAA CGG TAT CGA TAC TCC GCC GC -3', SEQ ID NO: 11], a ClaI restriction site was inserted between A and B parts by site-directed mutagenesis. Then, the AdEasy-1 plasmid as a template [5'-ATG AAG GGC ACG AGC TGC TT -3', SEQ ID NO: 12] and [5'-ATC AAC GAA TCC ACG TTG CGA C -3', SEQ ID NO: 13] A portion corresponding to (7892 - 9197) of SEQ ID NO: 1 was obtained by PCR and cloned into TOPO plasmid (Doctor protein, Cat #DR02202). Then [5'- GCA TTA ATC GAT ATG AAG GGC ACG AGC TGC TT -3', SEQ ID NO: 14] and [5'-GCA TTA ATC GAT ACC TAC ATC AAC GAA TCC ACG TTG CGA C -3', SEQ ID NO: 15] PCR was performed again on the TOPO plasmid using the primer. The ClaI restriction enzyme site and GTAGGT (SEQ ID NO: 16) at the (9640-9645) position are added to this primer. The PCR product was cut with ClaI and cloned into pGEM®-T Easy previously subjected to site-directed mutagenesis, and a portion involved in splicing of i-Leader and adenovirus RNA was inserted between portions A and B. Thereafter, in order to remove the ClaI restriction enzyme site used for cloning, the plasmid was converted to [5'-gtg gat tcg ttg atg tag gta ctc cgc cgc c-3', SEQ ID NO: 17] and [5'-ggc ggc gga gta cct aca tca acg aat cca c-3', SEQ ID NO: 18] was site-directed mutagenesis. The obtained plasmid was cut with BstXI, PmeI, and XmnI to obtain an insert, and the basic pShuttle plasmid included in the AdEasy-1 system was cut with BstXI and PmeI, and the two cut materials were attached with T4 ligase, respectively, and cloned to prepare a shuttle plasmid.

(2) Preparation Example 2

The vector of Preparation Example 2 has the same gene removal positions as the vector of Preparation Example 1 (7186..7891) and (9198..9639). However, C at position 8299 is replaced with T. Due to this, a stop codon is created in the protein made by i-Leader belonging to E2B and the structure of the protein is changed.

To construct the shuttle plasmid required for the vector construction of Preparation Example 2, the plasmid containing the synthesized sequence (SEQ ID NO: 19) was digested with BstZ17I and PmeI, and the basic pShuttle plasmid included in the AdEasy-1 system was digested with the same enzyme. . Each of the two cut materials was attached with T4 ligase and cloned to obtain a shuttle plasmid, and homologous recombination between the shuttle plasmid and pAdEasy-1 was induced to obtain an adenovirus plasmid in which the E2B part was modified.

(3) Production Example 3

The vector of Preparation Example 3 is a vector from which (7274..7881) and (9198..9630) have been removed. To construct the shuttle plasmid required for the vector construction of Preparation Example 3, artificially synthesized the E2B gene (SEQ ID NO: 20) from which the designated part was removed, and then cloned this gene in the same way as mentioned in the production process of Preparation Example 2 did. Thereafter, homologous recombination between the shuttle plasmid and pAdEasy-1 was induced to obtain an adenovirus plasmid in which the E2B portion was modified.

(4) Preparation Example 4

The vector of Preparation Example 4 is a vector in which (7274..7881) and (8583..9630) are removed, and compared with 3, the beginning and the end of the removal site are the same, but the inside is additionally removed. The shuttle plasmid used to prepare Preparation Example 4 was prepared using the same method as in Preparation Example 2 after synthesizing the sequence of SEQ ID NO: 21. Thereafter, homologous recombination with pAdEasy-1 was induced to obtain an adenovirus plasmid in which the E2B portion was modified.

(5) Production Example 5

The vector of Preparation Example 5 is a vector in which (7275..9632) has been removed, and more portions are removed compared to 4 as well. However, in the vector of Preparation Example 5, the left end of the removal site is 7275, not 7274, and the right end is 9632, not 9630, as in Preparation Example 3 or Preparation Example 4. The reason for this change is to prevent the Pol gene synthesis from continuing because the frame of gene synthesis starting from the pTP gene is not broken. The shuttle plasmid used to prepare Preparation Example 5 was prepared using the same method as in Preparation Example 2 after synthesizing the sequence of SEQ ID NO: 22. Thereafter, homologous recombination with pAdEasy-1 was induced to obtain an adenovirus plasmid in which the E2B portion was modified.

(6) Construction of HER2 loaded adenovirus plasmid

So far, based on the pAdEasy-1 plasmid, a method for constructing a new adenovirus plasmid in which a part of the E2B gene has been removed has been described. Most adenovirus plasmids, including pAdEasy-1, are designed to load a desired foreign gene into the E1 region. Here, using the pShuttle-CMV plasmid included in the AdEasyTM XL Adenoviral vector system (Agilent, Cat#240010), a method for loading the HER2 gene into the E1 part of the adenovirus plasmid in which a part of the E2B gene prepared previously was modified was described.

To obtain the human HER2 gene, RNA was extracted from T47D (ATCC), a breast cancer cell line, using Trizol (Invitrogen) reagent. RT-PCR was performed using the extracted RNA as a template using SuperScript III First-Strand Synthesis System (Invitrogen) to synthesize cDNA. In the synthesized cDNA, the HER2 portion was amplified using [Forward: 5'-CGATCG GGTACC GCCACC ATGGAGCTGGCGGCCTTGTG -3', SEQ ID NO: 23] and [Reverse: 5'-CGATCG AAGCTT TTACTA GATCCCAAAGACCACCCCCAAGA -3', SEQ ID NO: 24]. The amplified portion of HER2 is not the entire HER2 gene, but an extracellular region and a transmembrane region that does not have an intracellular region responsible for HER2 signal transduction (HER2). ECD-TM). In the forward primer, there is a Kozak sequence (Kozak sequence, GCCACC, SEQ ID NO: 25) known to increase gene expression in addition to the PvuI and KpnI restriction sites used for cloning, and the reverse primer includes the PvuI and HindIII restriction enzyme sites used for cloning, as well as the gene synthesis process a stop codon (TTACTA, SEQ ID NO: 26) for terminating the . The ECD-TM portion of the amplified HER2 was cloned into the intermediate plasmid pSP72 using Kpn I and Hind III restriction enzymes to construct pSP72-HER2, and the same Kpn I and Hind III enzymes were used to generate HER2 from pSP72-HER2. Separated to construct pShuttle-CMV-HER2. The process of loading HER2 into the adenovirus plasmid using pShuttle-CMV-HER2 was performed according to the manual ^{of the AdEasy TM XL Adenoviral vector system.}

(7) Confirmation of the structure of the prepared plasmid DNA

In order to confirm that the adenovirus plasmid DNA was constructed as expected, the plasmid DNA was digested with BstXI restriction enzyme and then confirmed by electrophoresis on an agarose gel. 1 is a result of BstXI analysis of the structure of the adenovirus plasmid DNA not loaded with HER2. The figure on the left is an expected result produced using the SNAPGENE program, and 1 to 5 correspond to Preparation Examples 1 to 5, respectively. MW is an abbreviation for molecular weight and is any DNA used to roughly estimate the size of each DNA. In this experiment, 1kb plus DNA ladder (Invitrogen) was used. In the figure on the right, it can be confirmed that a DNA band with the same size as the expected result exists as an actual experimental result. The same experiment was performed on the HER2 loaded plasmid DNA, and it was confirmed that a DNA band having the same size as the expected result was present ( FIG. 2 ). Through this, it was confirmed that there was no abnormality in the structure of the plasmid DNA used to construct the viral vector.

2. Construction of Packaging Cell Lines for Adenoviral Vector Production

The adenoviral vectors of Preparation Examples 1 to 5 are produced from plasmids from which E1 and E2B genes have been further removed as well as E1 removed in general adenovirus vectors. In order to produce a vector from such a plasmid, a cell line that can supplement the E2B gene from the outside is required so that the viral vector can self-replicate. In this patent, the pTP and Pol genes are delivered to HEK 293 cells using a retroviral vector. This produced a production cell line.

(1) cell culture

293 cells were obtained from ATCC, and FBS (Hyclone, Cat No. SH30084.03), L-glutamine (Gibco, Cat No. 25030-081) and Antibiotic- Antimycotic (Gibco, Cat No. 15240-062) was added to the culture medium. The final composition of the culture medium is 87% DMEM + 10% FBS + 4 mM L-glutamine (2%) + 1% Antibiotic-Antimycotic. Usually, subculture was performed about once every 3 to 4 days, and about 5 x 10 ⁵ to 1 x 10 ⁶ cells were placed in a 75-T flask. During subculture, the flask to which the cells were attached was lightly washed with PBS and then treated with Trypsin-EDTA to detach the cells. Cells were released in the separated culture medium, counted by the trypan blue method, and put into a new flask. 293T cells used for retrovirus production were also obtained from ATCC. 293, 293T, and 293E2B 5-2 cells, a production cell line derived from 293, were all cultured in the same manner as above.

(2) Securing a cell population expressing the E2B gene

The retroviral vector loaded with pTP and Pol genes was constructed based on the commercial retroviral plasmid pMSCV system (Clontech). The pTP gene was amplified using pAxCAwtit2 (Takara), a plasmid having an adenovirus genome, as a template. The amplified sequence contains non-coding DNA immediately preceding the protein-making sequence of the pTP gene (SEQ ID NO: 3), and the sequence is as follows (5'-ATG GCCTTGAGCG TCAACGATTG CGCGCGCCTG ACCGGCCAGA GCGTCCCGAC C -3', SEQ ID NO: 27), further contains a Kozak sequence (5'-GCCGCCACC -3', SEQ ID NO: 28) to increase the expression level immediately before the non-coding DNA, and EcoRI, a restriction enzyme site used for cloning, at both ends and BamHI seats are located. The amplified pTP gene was cloned into the pMSCV-Neo plasmid using the corresponding restriction enzyme to complete the pMSCV-pTP-Neo plasmid.

The Pol gene was amplified using the same DNA as a template. The amplified sequence identical to pTP contains non-coding DNA that does not produce a protein immediately before the protein-making sequence of the pol gene (SEQ ID NO: 2), and the sequence is as follows (5'-ATG GCC TTG GCTCAAGCTC ACCGGGCCCG TCGTCTTCAC GCAGAGGCGC) CAGATTCAGG AGATCAACCG CCGCGTCGTC GCGTTCGCCA GCAACCTACG CGCGCAGCAC CAGCTCCTGC CCGCGCGCGG CGCCGACGTG CCCCTGCCCC CTCTCCCGGC GGGTCCGGAG CCCCCCCTAC CTCCGGGGGC TCGCCCGCGT CACCGCTTTT AGATGCATCA TCCAAGGACA CCCCCGCGGC CCACCGCCCG CCGCGCGGTA CCGTAGTCGC GCCGCGGGGA TGCGGCCTCT TGCAAGCCAT CGACGCCGCC ACCAACCAGC CCCTGGAAAT TAGGTATCAC CTGGATCTAG CCCGCGCCCT GACCCGTCT ATGCGAGGTAA ACCTGCAGGA GCTCCCGCCT GACCTGACGC CGCGGGAGCT CCAGACC -3 ', SEQ ID NO: 29), additional non- It contains a Kozak sequence (5'-GCCGCCACC-3', identical to SEQ ID NO: 28) for increasing the expression level immediately before the encoding DNA. Restriction enzyme sites used for cloning, BglII and HpaI, are located at both ends. The amplified pTP gene was cloned into the pMSCV-Hyg plasmid using BglII and HpaI restriction enzymes to complete the pMSCV-Pol-Hyg plasmid.

293T cells were transfected with the two completed retroviral plasmids and pGP and pE, which are components of the Retroviral packaging kit Ampho (Takara), a commercial retroviral production kit, to produce a retroviral vector. For transfection, Mirus' TransIT-293 product was used. Two days after transfection, a culture solution of 293T cells was obtained, purified through a syringe filter having a size of 0.45 μm, and then treated with 293 cells to transform the cells. During transformation, a Pol gene-expressing retroviral vector is first infected, transformed to express the Pol gene, and then the cells are infected with a pTP-expressing retroviral vector and transformed cell population expressing both pTP and Pol. got To select only transformed cells, cells infected with MSCV-pTP-Neo vector were treated with G418 at 600 μg/mL, and cells infected with MSCV-Pol-Hyg vector were treated with Hygromycin at 100 μg/mL.

(3) Securing production cell lines

A well-known limiting dilution method was used to secure one production cell line clone from the thus-obtained cell population. After counting and seeding the 96-well plate so that 0.5 cells per well were seeded, those in which a single cell mass was confirmed when observed under a microscope after about 2 weeks were selected. After securing about 100 such candidate clones, a total of 6 candidate clones having higher productivity than other clones were obtained. After that, for 6 clones, the pTP and Pol genes were well expressed, and the productivity and growth rate were maintained constant while culturing for about 3 months, and the sequences of pTP and Pol and the surrounding retroviral vectors were analyzed for mutations. . Through this, the 293E2B 5-2 cell line, which is the final cell line that passed all the assays, was obtained. All packaging cell lines used in the production of adenoviral vectors in this patent are 293E2B 5-2.

3. Biochemical analysis of adenoviral vectors

(1) Construction of adenoviral vector

Construction of the adenoviral vector was started from the adenoviral plasmid of Example 1. First, the adenovirus plasmid DNA was cut using PacI restriction enzyme to obtain a linear vector DNA including ITR sequences on both sides, and then purified using phenol-chloroform. 2.5 μg of the obtained digested DNA was transfected into the packaging cell line using TransIT-293 reagent (MirusBio). At the time when the cytopathic effect (CPE) is sufficiently visible (it takes about 2 weeks), both the cells and the culture medium were harvested and subjected to freezing (-80 ℃, 1 hour or more) and thawing (37 ℃ constant temperature water bath, 20 minutes) three times. After that, only the supernatant was filtered with a 0.22 μm PVDF membrane, and the process of re-infecting the packaging cells to amplify the viral vector was repeated. The amplification of the viral vector was scale-up to the level of 6-well, T-25, T-75, and T-175, and the culture medium was 5% DMEM solution (92% DMEM + 5% FBS + 4 mM) L-glutamine (2%) + 1% Antibiotic-Antimycotic) was used. The titer of the produced adenovirus vector was _{measured using the well-known TCID 50} method or QuickTiter ^TM adenovirus titer immunoassay kit (Cell Biolabs). The adenoviral vector produced in this way is in the form of a crude mixture of cell-derived material and viral vector.

(2) Virus infection and biochemical analysis

Whether or not the function of the E2B gene was lost due to various genetic modifications introduced in Preparation Example 1 using the produced adenovirus vector was verified by confirming the late gene expression of the virus. 24 hours before infection, the packaging cell lines were inoculated into a collagen type I-coated 6-well plate so ^{that each cell line was 3 x 10 5 cells/well.} One day later, each vector produced in 3-1 was transfected into cells at 1 MOI (Ifu/cell). As a control, the Ad-GFP vector in which the E2B portion was not removed was infected with 293 cells at _{1 MOI (TCID 50 /cell).} The Ad-GFP vector is Invitrogen's pAd/CMV/V5-DEST?? Gateway?? It was produced using Vector Kit. On the third day after infection, the media and cells were harvested, centrifuged, and the protein was obtained from the cell pellet with proprep (Invitrogen, Cat No. 17081). After protein quantification by Bradford assay, SDS-PAGE was performed on 8% gel using 20 μg protein. After transfer to the membrane by the wet transfer method, blocking was performed using a blocking reagent (Bio-Rad) diluted to 5% in TBST. After that, the primary antibody was diluted in the same blocking solution and reacted in an inverter at 4 ℃ for 16 hours. These types and dilution ratios are anti-wt Ad5 antibody (Abfrontier) (1:2000), anti-Ad5 Hexon antibody (Abfrontier, Cat No. LF-MA0177) (1:2000), anti-β-actin antibody (Sigma) Aldrich, Cat No. A1978) (1:10000). For the anti-β-actin antibody, blocking and antibody dilution were performed with an exception of 1% blocking reagent. The next day, the membrane was washed 3 times with TBST and reacted with a secondary antibody suitable for the primary antibody. The secondary antibodies used were anti-rabbit secondary antibody (Abcam, Cat# No.ab6721) and anti-mouse secondary antibody (Abcam, Cat# No.ab6728), and the dilution ratio was slightly different depending on the primary antibody, but 1:2000 - It was diluted with the blocking reagent at the same % as the dilution of the primary antibody between 1:5000. After the reaction with the secondary antibody, the membrane was washed 3 times with TBST, and the result was confirmed by reaction with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare, Cat No. RPN2232).

3 shows the results of analysis of late viral proteins after infecting 293 cells with an E2B-removed adenovirus vector and a control, Ad-GFP vector. When Ad-GFP in which only E1 has been removed is infected with 293 cells including E1, viral DNA replication occurs normally and late virus genes are expressed. You can see this on Lane 2. If the E2B gene other than E1 is further damaged, virus replication does not occur even after infection with 293 cells, so the late virus gene is not expressed. You can see this on Lane 3-7. That is, in the vectors of Preparation Examples 1 to 5, it can be seen that not only the E2B gene was removed, but also the function of the E2B gene was blocked. Even in a virus in which the E2B gene has been deleted, virus replication can proceed if the function of the E2B gene is provided from the outside. 4 shows that the packaging cell line expressing the E2B gene in addition to the E1 gene was infected with the same viral vector as used in FIG. 3 , and it can be confirmed that viral DNA replication occurs in all infected groups and the late gene is expressed.

4. Genetic Stability Analysis of Adenoviral Vectors

(1) PCR structural analysis of the E2B portion of the viral vector that is not loaded with a foreign gene

In order to confirm that the DNA structure of the produced adenovirus vector matches the DNA structure of the plasmid, the adenovirus vector having the E2B structure of Preparation Example 1-5 was infected with the packaging cell line. The adenoviral vector production method and infection method are the same as those mentioned in Example 2.

The infected cells were harvested on the 3rd day after infection, and the genomic DNA of the viral vector was extracted using the Gentra Puregene Cell kit (Qiagen, Cat No. 158767). Using 1 μl of the extracted gDNA as a template, using the primers shown below (Forward primer: 5'-GCTCGGAAGACTATCTGCCT -3' (SEQ ID NO: 30), Reverse primer: 5'-TACTCGCGTCTCAGGTACAC -3' (SEQ ID NO: 31)) E2B region was amplified by PCR. For PCR, PrimeSTAR Max (TaKaRa, Cat No. R045) was used, and it was performed with a Biometra TRIO (Analytikjena) device, and the cycling parameters are as follows: 95 ℃ 5 min once -> [98 ℃ 10 min -> 57 ℃ 5 sec -> 72 ℃ 30 sec] (repeat 30 times) -> 72 ℃ 5 min once. At this time, 100 ng of the adenovirus plasmid used to produce each viral vector was reacted in the same manner to be used as a positive control. In order to check the possibility of external contamination during the PCR experiment, a PCR reaction was performed by adding only water, not plasmid DNA or genomic DNA of a viral vector, and this was indicated as NTC. 10% volume of the reaction was confirmed by electrophoresis on 1% agarose gel. When having the E2B structure of each preparation example, the expected size of the PCR product is 2263 bp (Preparation Example 1), 2263 bp (Preparation Example 2), 2365 bp (Preparation Example 3), 1750 bp (Preparation Example 4), 1048 bp ( Preparation Example 5).

The upper figure and the lower figure of FIG. 5 are taken differently by adjusting only the intensity of the same figure, respectively. No amplification is observed in NTC, and it can be confirmed that PCR amplification products of the same size as plasmid DNA for all viral vectors are observed in PCR using genomic DNA of viral vectors. In order to check whether the PCR amplification product thus obtained corresponds to the actual E2B part, a band of the corresponding size was separated using the Wizard SV gel and PCR clean up system (Promega), and then sequencing was performed to confirm that the actual E2B sequence was correct. . This shows that the E2B portion of the DNA of the viral vector has the same structure as the plasmid. However, looking at the experimental results using the viral vector of Preparation Example 2 shown in lane 5, PCR amplification products of a small size that were not observed when PCR was performed with a plasmid were confirmed when PCR was performed using DNA derived from a viral vector. can be seen (indicated by the red arrow). When it was found that no PCR product different from the expected size was identified in the plasmid, which is the initial material for production, this result suggests that a viral vector with an unintentionally modified E2B part structure was created during the production process.

To confirm the above results, subculture of the adenovirus vector having the E2B structure of Preparation Example 2 was performed. This is amplification by additionally infecting the previously produced viral vector with the packaging cell line. If a viral vector having a different actual structure is mixed, it can be expected to remain the same even after subculture or to be further amplified. The packaging cell line was infected with the viral vector having the E2B structure of Preparation Example 2 at 10 Ifu/ml, and cells and culture medium were harvested on the 3rd day of infection showing CPE, freezing (-80 ° C, 1 hour or more) and thawing (37 ℃ constant temperature water bath, 20 minutes) was repeated 4 times, and then filtered through a 0.22 μm PVDF membrane to complete the first subculture. The initial viral vector was denoted as P0, and the vector after the first subculture was denoted as P1. Cells harvested during subculture were analyzed for the structure of the E2B portion in the same manner as in the experiment of FIG. 5 . In order to obtain the gDNA of the virus, TAKARA miniBEST viral RNA and DNA extraction kit (TAKARA, Cat No. 9766) was additionally used in addition to the previously used Gentra Puregene Cell kit. As a result of the experiment, in the plasmid DNA corresponding to Preparation Example 2, only a band of 2263 bp was observed when PCR was performed, but in addition to the band of 2263 bp in the DNA of the viral vector, the size was smaller than expected regardless of how the viral gDNA was obtained. PCR amplification products were observed together. This result shows that the viral vector having the E2B structure of Preparation Example 2 has low genetic safety, and thus a viral vector having a slightly different structure of the E2B portion is generated during production ( FIG. 6 ).

(2) PCR structural analysis of the E2B portion of the viral vector loaded with a foreign gene (HER2)

The genetic stability of the vector having the E2B structure of Preparation Example 1-5 and loading HER2 as a foreign gene in the E1 region was also observed in the same manner. The method of loading HER2 on the adenovirus plasmid having the E2B structure of Preparation Example 1-5 is presented in Example 1 (6). A viral vector was produced from the adenovirus plasmid thus made, and the production method was presented in (1) of Example 3. The produced viral vector was infected with a packaging cell line, and a PCR reaction was performed to obtain gDNA of the viral vector. The detailed analysis method is the same as (1) of Example 4, and the results are shown in FIG. 7 .

As a result of the experiment, no amplification was observed in NTC, and it was confirmed that PCR amplification products of the same size as the plasmid DNA were observed during PCR using the vector DNA for the remaining viral vectors except for Preparation Example 1. In other words. It can be seen that the DNA of these viral vectors has an E2B portion of the same structure as the plasmid DNA. In the case of the adenoviral vector having the structure of Preparation Example 1 and loaded with HER2, PCR amplification product of a size smaller than expected in addition to the size consistent with the plasmid DNA similar to that previously observed for the E2B structure of Preparation Example 2 in FIGS. 5 and 6 was observed (Figure 7). In the viral vector having the E2B structure of Preparation Example 2 and loaded with HER2, no structural abnormality was observed in the E2B portion of the DNA, unlike the vector without HER2.

The PCR amplification product of a smaller size than the expected size observed in FIG. 7 had a larger amount than was previously confirmed in FIG. 5 , such as having a dark intensity, so a more detailed analysis was performed. In order to confirm the exact sequence of this part, a small-sized PCR band region was extracted and sequenced using Wizard SV gel and PCR clean-up system. For the sequencing method, the PCR product is inserted into the T-vector, and then M13-F (5'-GTAAAACGACGGCCAGT-3', SEQ ID NO: 32), M13-R (5'-GCGGATAACAATTTCACACAGG-3', SEQ ID NO: 33) primers are used. to read the sequence. The obtained sequence was analyzed using SNAPGENE, a DNA sequence analysis program. As a result, it was found that the small size of the PCR product was the E2B part, but a part in the middle was cut ( FIG. 8 ).

Another way to check the DNA structure of a viral vector is to cut the DNA with a restriction enzyme and compare the expected results. Restriction enzyme analysis was performed on the DNA of the viral vector loaded with HER2 while having the E2B structure of Preparation Example 1. Restriction enzymes used for the analysis are BstXⅠ (NEB, Cat No. R0133S) and DraⅠ (NEB, Cat No. R0129S), and 1 μg of viral genomic DNA is mixed with 1 μL of restriction enzyme and reacted in a water bath at 37 ° C for 2 hours. , and observed the results by electrophoresis on 0.6% and 1% agarose gels. The results of electrophoresis on two gels are shown, and on the left of each result, the expected results when the virus was cut with BstXI (1) and Dra I (2) are shown. The expected results were generated using the SNAPGENE program. MW is an abbreviation for molecular weight, and is arbitrary DNA used to roughly estimate the size of each DNA. In this experiment, 1kb plus DNA ladder (Invitrogen) was used.

As a result of the experiment, unexpected bands were observed at about 3 kb when reacting with BstXI, and at about 7 kb when reacting with DraI ( FIG. 9 , red box). This result shows that the structure of the corresponding viral vector is different from that expected from the plasmid, as in the previous PCR experiment.

Summarizing the results of the analysis so far, the viral vectors having the E2B structure of all production examples had the E2B gene function broken, but if they had the E2B structure corresponding to Preparation Examples 1 and 2, they would have been produced in the absence or presence of HER2, respectively. When the structure of the E2B portion of the vector DNA is partially unstable, a phenomenon was observed.

The structure of the viral vector having the E2B structure of Preparation Examples 3 to 5, which has excellent genetic stability while the function of E2B is broken, was further analyzed by a restriction enzyme treatment method. The viral vector loaded with HER2 was first analyzed, and the enzyme used for the analysis was BstXⅠ, and the viral gDNA obtained from each vector was reacted with BstXII in a constant temperature water bath at 37 °C for 3 hours and then electrophoresed on a 1% agarose gel. was confirmed (FIG. 10).

The leftmost figure in FIG. 10 is the expected result prepared using the SNAPGENE program, and 1, 2, and 3 correspond to Preparation Examples 3-5, respectively. In the figure on the right, three photos taken with the same photo intensity adjusted are displayed as the actual experimental results. Results corresponding to Preparation Examples 3 to 5, respectively, from the left except for the size marker for indicating the size of DNA. It can be seen that when the DNA of all three viral vectors was cut with BstXI, bands of the size indicated in the expected results were confirmed. However, when the DNA of the viral vector having the structure of Preparation Examples 3 and 5 was cut, the electrophoresis result was clean, whereas when the viral vector DNA having the structure of Preparation Example 4 was electrophoresed, the overall appearance was messy, and therefore the most The lower band was not clearly visible. In Table 1, the size of the cleaved DNA presented in the expected results is indicated, and whether the DNA of the corresponding size is visually confirmed in the results of FIG. 10 is indicated by O and X. The DNA structure was analyzed in the same manner for vectors having the E2B structure of Preparation Examples 3-5 and not loaded with HER2, and it was confirmed that the same structure as expected was obtained. As a result, it was found that the viral vectors having the E2B structure of Preparation Examples 3-5 had no structural abnormalities in the vector DNA regardless of whether foreign genes were loaded or not.

Expected digested size (bp) 19AD-02
(Production Example 4) 19AD-06
(Production Example 5) 19AD-09
(Production Example 6) 11,920 O 11,920 O 11,920 O 4,254 O 4,254 O 4,254 O 4,210 3,595 O 3,470 O 3,471 O 3,471 3,385 3,385 3,385 2,893 O 2,401 O 2,401 O 2,401 O 1,393 O 1,393 O 1,393 O 960 O 960 O 960 O

5. Purification and Analysis of Adenoviral Vectors

The viral vectors used so far for biochemical or DNA structure analysis of viral vectors are in the form of crude viruses in which viral vectors and cell-derived substances are mixed. When applied to animal experiments or actual humans, a large amount of crude virus vector is obtained and then purified and used. In this example, a large amount of crude virus of the viral vector having the E2B structure of Preparation Examples 3 to 5 was obtained and purification was attempted. As an initial material, an adenovirus vector having the E2B structure of Preparation Example 3-5 and having a HER2 loaded structure was used.

In order to obtain a large amount of crude virus vector, 30 packaging cell lines were prepared in a T-175 flask, and each flask was infected with the crude virus vector at 5 ifu/cell. Cells were seeded into 1.2 X 10 ⁷ cells/T-175 flask, infected on the first day after seeding, and both cells and culture medium were harvested on the third day after infection. After that, freezing (-80 °C, 1 hour or more) and thawing (37 °C constant temperature water bath, 20 to 30 minutes) were repeated three times. After repeating 3 times, centrifugation was performed to secure only the supernatant and filtered through a 0.45 μm membrane.

It is well known that adenoviral vectors can be separated and purified by density difference by making a density gradient using CsCl (cesium chloride) aqueous solutions of different concentrations and centrifuging. Briefly, 4 mL of a 1.4 g/mL aqueous solution of CsCl (Sigma Aldrich, Cat No. C6914) and 3.5 mL of a 1.2 g/mL aqueous solution are placed in an ultracentrifuge tube in order, and then the filtered supernatant is placed on it. About 4.5 mL was added. Ultracentrifuge was performed for 2 to 3 hours at 24000 rpm at 4 °C with the tube almost full. As shown in the photo of FIG. 11, after centrifugation, two bands can be observed. Of these, the lower band was obtained using a syringe. In the case of having the E2B structure of Preparation Examples 3 and 5, a clear band could be identified, and the vector having the E2B structure of Preparation Example 4 was dark, but still had a band ( FIG. 11 ).

In order to proceed with purification once more, 7.5 mL of 1.35 g/mL CsCl solution was put into an ultracentrifuge tube, and the adenovirus vector obtained earlier was added thereto. Ultracentrifuge was performed overnight at 24000 rpm at 4°C with the tube almost full. One band observed after centrifugation was obtained using a syringe. After putting the obtained band in Slide-A-Lyzer G2 Dialysis Cassettes (Thermo Scientific, Cat No. 88251) pre-incubated in dialysis buffer, press the center to remove air bubbles, and then close the entrance to proceed with dialysis. Dialysis was repeated twice in 1X dialysis buffer for 2-3 hours, and dialysis was performed once overnight. The buffer used for overnight dialysis contains 5% sucrose. After overnight dialysis, the adenovirus vector was harvested using a syringe, aliquoted in 10 to 20 μl, and stored at -80°C.

To check whether the purification was successful, the titer of the purified virus (virus particles, number of virus particles) _{was calculated by the well-known A 260} measurement method. In this method, after removing the virus capsid protein using sodium dodecyl sulfate (SDS), the amount of viral DNA is measured by _{irradiating light with a wavelength of 260 nm that DNA can absorb well and measuring the absorbance A 260 value.} How to convert it to a potency. As a result of the measurement, the purified adenovirus vectors having the E2B structure of Preparation Examples 3 and 5 ^{had titers of 5.40 X 10 11} VP/mL and 4.68 X 10 ¹¹ VP/mL, respectively. The absorbance of the viral vector having the structure of Preparation Example 4 was measured, but the exact titer could not be confirmed because the value was below the detection limit of the current measurement method. This result shows that it is possible to secure a purified vector with respect to the viral vector having the E2B structure of Preparation Examples 3, 4, and 5 and loaded with an external gene.

DNA was also obtained from the purified virus and the structures of the E2B region and the HER2 region were analyzed by PCR. First, to obtain adenoviral vector DNA, 5 µL of viral vector was diluted with 95 µL of DPBS (Welgene), and then 5 µL of proteinase K (Qiagen, Material No. 1019499) was added. The DNA of the viral vector was extracted by sequentially reacting at 55 °C for 1 hour and at 94 °C for 30 minutes. The PCR amplification method of the E2B part is the same as that mentioned in (1) of 4. Detection of the loaded foreign gene, HER2, was amplified using the primers shown below (Forward primer: 5'-GGTCTATATAAGCAGAGCTG-3' (SEQ ID NO: 40), Reverse primer: 5'-GTGGTATGGCTGATTATGATCAG-3' (SEQ ID NO: 41)) . For PCR, PrimeSTAR Max (TaKaRa, Cat No. R045) was used, and it was performed with a Biometra TRIO (Analytikjena) device, and the cycling parameters are as follows: 95 ℃ 5 min once -> [98 ℃ 10 min -> 55 ℃ 5 sec -> 72 ℃ 13 sec] (repeat 35 times) -> 72 ℃ 10 min once. At this time, 1 μL of the extracted viral vector DNA was used as a template, and 100 ng of the adenovirus plasmid used to produce each viral vector as a positive control was reacted in the same manner. In order to check the possibility of external contamination during the PCR experiment, a PCR reaction was performed by adding only water, not plasmid DNA or viral DNA, and this was indicated as NTC. 20% volume of the reaction was confirmed by electrophoresis on 1% agarose gel. The expected size of the PCR product of the E2B portion of each Preparation Example is 2365 bp (Preparation Example 3), 1750 bp (Preparation Example 4), and 1048 bp (Preparation Example 5), and the expected size of the PCR product of the HER2 portion is Preparation Example 3, It is 2153 bp in both Preparation Example 4 and Preparation Example 5.

12, it can be confirmed that no amplification is observed in NTC, and PCR amplification products of the same size as the plasmid DNA are observed during PCR amplification using the vector DNA for the vectors having the E2B structure of all preparation examples. This shows that the adenoviral vector having the E2B structure of Preparation Example 3-5 maintains genetic stability even after purification.

<110> KOLON LIFE SCIENCE, INC. <120> ADENOVIRUS VECTOR <130> 10096 <160> 41 <170> KoPatentIn 3.0 <210> 1 <211> 35934 <212> DNA <213> Homo sapiens <400> 1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagatgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccat gagcaccaac tcgtttgatg gaagcattgt gagctcatat ttgacaacgc 3660 gcatgccccc atgggccggg gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc 3720 ccgtcctgcc cgcaaactct actaccttga cctacgagac cgtgtctgga acgccgttgg 3780 agactgcagc ctccgccgcc gcttcagccg ctgcagccac cgcccgcggg attgtgactg 3840 actttgcttt cctgagcccg cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg 3900 acaagttgac ggctcttttg gcacaattgg attctttgac ccgggaactt aatgtcgttt 3960 ctcagcagct gttggatctg cgccagcagg tttctgccct gaaggcttcc tcccctccca 4020 atgcggttta aaacataaat aaaaaaccag actctgtttg gatttggatc aagcaagtgt 4080 cttgctgtct ttatttaggg gttttgcgcg cgcggtaggc ccgggaccag cggtctcggt 4140 cgttgagggt cctgtgtatt ttttccagga cgtggtaaag gtgactctgg atgttcagat 4200 acatgggcat aagcccgtct ctggggtgga ggtagcacca ctgcagagct tcatgctgcg 4260 gggtggtgtt gtagatgatc cagtcgtagc aggagcgctg ggcgtggtgc ctaaaaatgt 4320 ctttcagtag caagctgatt gccaggggca ggcccttggt gtaagtgttt acaaagcggt 4380 taagctggga tgggtgcata cgtggggata tgagatgcat cttggactgt atttttaggt 4440 tggctatgtt cccagccata tccctccggg gattcatgtt gtgcagaacc accagcacag 4500 tgtatccggt gcacttggga aatttgtcat gtagcttaga aggaaatgcg tggaagaact 4560 tggagacgcc cttgtgacct ccaagatttt ccatgcattc gtccataatg atggcaatgg 4620 gcccacgggc ggcggcctgg gcgaagatat ttctgggatc actaacgtca tagttgtgtt 4680 ccaggatgag atcgtcatag gccattttta caaagcgcgg gcggagggtg ccagactgcg 4740 gtataatggt tccatccggc ccaggggcgt agttaccctc acagatttgc atttccccacg 4800 ctttgagttc agatgggggg atcatgtcta cctgcggggc gatgaagaaa acggtttccg 4860 gggtagggga gatcagctgg gaagaaagca ggttcctgag cagctgcgac ttaccgcagc 4920 cggtgggccc gtaaatcaca cctattaccg gctgcaactg gtagttaaga gagctgcagc 4980 tgccgtcatc cctgagcagg ggggccactt cgttaagcat gtccctgact cgcatgtttt 5040 ccctgaccaa atccgccaga aggcgctcgc cgcccagcga tagcagttct tgcaaggaag 5100 caaagttttt caacggtttg agaccgtccg ccgtaggcat gcttttgagc gtttgaccaa 5160 gcagttccag gcggtcccac agctcggtca cctgctctac ggcatctcga tccagcatat 5220 ctcctcgttt cgcgggttgg ggcggctttc gctgtacggc agtagtcggt gctcgtccag 5280 acgggccagg gtcatgtctt tccacgggcg cagggtcctc gtcagcgtag tctgggtcac 5340 ggtgaagggg tgcgctccgg gctgcgcgct ggccagggtg cgcttgaggc tggtcctgct 5400 ggtgctgaag cgctgccggt cttcgccctg cgcgtcggcc aggtagcatt tgaccatggt 5460 gtcatagtcc agcccctccg cggcgtggcc cttggcgcgc agcttgccct tggaggaggc 5520 gccgcacgag gggcagtgca gacttttgag ggcgtagagc ttgggcgcga gaaataccga 5580 ttccggggag taggcatccg cgccgcaggc cccgcagacg gtctcgcatt ccacgagcca 5640 ggtgagctct ggccgttcgg ggtcaaaaac caggtttccc ccatgctttt tgatgcgttt 5700 cttacctctg gtttccatga gccggtgtcc acgctcggtg acgaaaaggc tgtccgtgtc 5760 cccgtataca gacttgagag gcctgtcctc gagcggtgtt ccgcggtcct cctcgtatag 5820 aaactcggac cactctgaga caaaggctcg cgtccaggcc agcacgaagg aggctaagtg 5880 ggaggggtag cggtcgttgt ccactagggg gtccactcgc tccagggtgt gaagacacat 5940 gtcgccctct tcggcatcaa ggaaggtgat tggtttgtag gtgtaggcca cgtgaccggg 6000 tgttcctgaa ggggggctat aaaagggggt gggggcgcgt tcgtcctcac tctcttccgc 6060 atcgctgtct gcgagggcca gctgttgggg tgagtactcc ctctgaaaag cgggcatgac 6120 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccgc 6180 ggtgatgcct ttgagggtgg ccgcatccat ctggtcagaa aagacaatct ttttgttgtc 6240 aagcttggtg gcaaacgacc cgtagagggc gttggacagc aacttggcga tggagcgcag 6300 ggtttggttt ttgtcgcgat cggcgcgctc cttggccgcg atgtttagct gcacgtattc 6360 gcgcgcaacg caccgccatt cgggaaagac ggtggtgcgc tcgtcgggca ccaggtgcac 6420 gcgccaaccg cggttgtgca gggtgacaag gtcaacgctg gtggctacct ctccgcgtag 6480 gcgctcgttg gtccagcaga ggcggccgcc cttgcgcgag cagaatggcg gtagggggtc 6540 tagctgcgtc tcgtccgggg ggtctgcgtc cacggtaaag accccgggca gcaggcgcgc 6600 gtcgaagtag tctatcttgc atccttgcaa gtctagcgcc tgctgccatg cgcgggcggc 6660 aagcgcgcgc tcgtatgggt tgagtggggg accccatggc atggggtggg tgagcgcgga 6720 ggcgtacat ccgcaaatgt cgtaaacgta gaggggctct ctgagtattc caagatatgt 6780 agggtagcat cttccaccgc ggatgctggc gcgcacgtaa tcgtatagtt cgtgcgaggg 6840 agcgaggagg tcgggaccga ggttgctacg ggcgggctgc tctgctcgga agactatctg 6900 cctgaagatg gcatgtgagt tggatgatat ggttggacgc tggaagacgt tgaagctggc 6960 gtctgtgaga cctaccgcgt cacgcacgaa ggaggcgtag gagtcgcgca gcttgttgac 7020 cagctcggcg gtgacctgca cgtctagggc gcagtagtcc agggtttcct tgatgatgtc 7080 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 7140 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtaagagc ctagcatgta 7200 gaactggttg acggcctggt aggcgcagca tcccttttct acgggtagcg cgtatgcctg 7260 cgcggccttc cggagcgagg tgtgggtgag cgcaaaggtg tccctgacca tgactttgag 7320 gtactggtat ttgaagtcag tgtcgtcgca tccgccctgc tcccagagca aaaagtccgt 7380 gcgctttttg gaacgcggat ttggcagggc gaaggtgaca tcgttgaaga gtatctttcc 7440 cgcgcgaggc ataaagttgc gtgtgatgcg gaagggtccc ggcacctcgg aacggttgtt 7500 aattacctgg gcggcgagca cgatctcgtc aaagccgttg atgttgtggc ccacaatgta 7560 aagttccaag aagcgcggga tgcccttgat ggaaggcaat tttttaagtt cctcgtaggt 7620 gagctcttca ggggagctga gcccgtgctc tgaaagggcc cagtctgcaa gatgagggtt 7680 ggaagcgacg aatgagctcc acaggtcacg ggccattagc atttgcaggt ggtcgcgaaa 7740 ggtcctaaac tggcgaccta tggccatttt ttctggggtg atgcagtaga aggtaagcgg 7800 gtcttgttcc cagcggtccc atccaaggtt cgcggctagg tctcgcgcgg cagtcactag 7860 aggctcatct ccgccgaact tcatgaccag catgaagggc acgagctgct tcccaaaggc 7920 ccccatccaa gtataggtct ctacatcgta ggtgacaaag agacgctcgg tgcgaggatg 7980 cgagccgatc gggaagaact ggatctcccg ccaccaattg gaggagtggc tattgatgtg 8040 gtgaaagtag aagtccctgc gacgggccga acactcgtgc tggcttttgt aaaaacgtgc 8100 gcagtactgg cagcggtgca cgggctgtac atcctgcacg aggttgacct gacgaccgcg 8160 cacaaggaag cagagtggga atttgagccc ctcgcctggc gggtttggct ggtggtcttc 8220 tacttcggct gcttgtcctt gaccgtctgg ctgctcgagg ggagttacgg tggatcggac 8280 caccacgccg cgcgagccca aagtccagat gtccgcgcgc ggcggtcgga gcttgatgac 8340 aacatcgcgc agatgggagc tgtccatggt ctggagctcc cgcggcgtca ggtcaggcgg 8400 gagctcctgc aggtttacct cgcatagacg ggtcagggcg cgggctagat ccaggtgata 8460 cctaatttcc aggggctggt tggtggcggc gtcgatggct tgcaagaggc cgcatccccg 8520 cggcgcgact acggtaccgc gcggcgggcg gtgggccgcg ggggtgtcct tggatgatgc 8580 atctaaaagc ggtgacgcgg gcgagccccc ggaggtaggg ggggctccgg acccgccggg 8640 agagggggca ggggcacgtc ggcgccgcgc gcgggcagga gctggtgctg cgcgcgtagg 8700 ttgctggcga acgcgacgac gcggcggttg atctcctgaa tctggcgcct ctgcgtgaag 8760 acgacgggcc cggtgagctt gaacctgaaa gagagttcga cagaatcaat ttcggtgtcg 8820 ttgacggcgg cctggcgcaa aatctcctgc acgtctcctg agttgtcttg ataggcgatc 8880 tcggccatga actgctcgat ctcttcctcc tggagatctc cgcgtccggc tcgctccacg 8940 gtggcggcga ggtcgttgga aatgcgggcc atgagctgcg agaaggcgtt gaggcctccc 9000 tcgttccaga cgcggctgta gaccacgccc ccttcggcat cgcgggcgcg catgaccacc 9060 tgcgcgagat tgagctccac gtgccgggcg aagacggcgt agtttcgcag gcgctgaaag 9120 aggtagttga gggtggtggc ggtgtgttct gccacgaaga agtacataac ccagcgtcgc 9180 aacgtggatt cgttgatatc ccccaaggcc tcaaggcgct ccatggcctc gtagaagtcc 9240 acggcgaagt tgaaaaactg ggagttgcgc gccgacacgg ttaactcctc ctccagaaga 9300 cggatgagct cggcgacagt gtcgcgcacc tcgcgctcaa aggctacagg ggcctcttct 9360 tcttcttcaa tctcctcttc cataagggcc tccccttctt cttcttctgg cggcggtggg 9420 ggagggggga cacggcggcg acgacggcgc accgggaggc ggtcgacaaa gcgctcgatc 9480 atctccccgc ggcgacggcg catggtctcg gtgacggcgc ggccgttctc gcgggggcgc 9540 agttggaaga cgccgcccgt catgtcccgg ttatgggttg gcggggggct gccatgcggc 9600 agggatacgg cgctaacgat gcatctcaac aattgttgtg taggtactcc gccgccgagg 9660 gacctgagcg agtccgcatc gaccggatcg gaaaacctct cgagaaaggc gtctaaccag 9720 tcacagtcgc aaggtaggct gagcaccgtg gcgggcggca gcgggcggcg gtcggggttg 9780 tttctggcgg aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag acggcggatg 9840 gtcgacagaa gcaccatgtc cttgggtccg gcctgctgaa tgcgcaggcg gtcggccatg 9900 ccccaggctt cgttttgaca tcggcgcagg tctttgtagt agtcttgcat gagcctttct 9960 accggcactt cttcttctcc ttcctcttgt cctgcatctc ttgcatctat cgctgcggcg 10020 gcggcggagt ttggccgtag gtggcgccct cttcctccca tgcgtgtgac cccgaagccc 10080 ctcatcggct gaagcagggc taggtcggcg acaacgcgct cggctaatat ggcctgctgc 10140 acctgcgtga gggtagactg gaagtcatcc atgtccacaa agcggtggta tgcgcccgtg 10200 ttgatggtgt aagtgcagtt ggccataacg gaccagttaa cggtctggtg acccggctgc 10260 gagagctcgg tgtacctgag acgcgagtaa gccctcgagt caaatacgta gtcgttgcaa 10320 gtccgcacca ggtactggta tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc 10380 cagcgtaggg tggccggggc tccgggggcg agatcttcca acataaggcg atgatatccg 10440 tagatgtacc tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg 10500 cggacgcggt tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg 10560 ccggtcaggc gcgcgcaatc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg 10620 ggcactcttc cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt 10680 tcgagccccg tatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc 10740 caggtgtgcg acgtcagaca acgggggagt gctccttttg gcttccttcc aggcgcggcg 10800 gctgctgcgc tagctttttt ggccactggc cgcgcgcagc gtaagcggtt aggctggaaa 10860 gcgaaagcat taagtggctc gctccctgta gccggagggt tattttccaa gggttgagtc 10920 gcgggacccc cggttcgagt ctcggaccgg ccggactgcg gcgaacgggg gtttgcctcc 10980 ccgtcatgca agaccccgct tgcaaattcc tccggaaaca gggacgagcc ccttttttgc 11040 ttttcccaga tgcatccggt gctgcggcag atgcgccccc ctcctcagca gcggcaagag 11100 caagagcagc ggcagacatg cagggcaccc tcccctcctc ctaccgcgtc aggaggggcg 11160 acatccgcgg ttgacgcggc agcagatggt gattacgaac ccccgcggcg ccgggcccgg 11220 cactacctgg acttggagga gggcgagggc ctggcgcggc taggagcgcc ctctcctgag 11280 cggcacccaa gggtgcagct gaagcgtgat acgcgtgagg cgtacgtgcc gcggcagaac 11340 ctgtttcgcg accgcgaggg agaggagccc gaggagatgc gggatcgaaa gttccacgca 11400 gggcgcgagc tgcggcatgg cctgaatcgc gagcggttgc tgcgcgagga ggactttgag 11460 cccgacgcgc gaaccgggat tagtcccgcg cgcgcacacg tggcggccgc cgacctggta 11520 accgcatacg agcagacggt gaaccaggag attaactttc aaaaaagctt taacaaccac 11580 gtgcgtacgc ttgtggcgcg cgaggaggtg gctataggac tgatgcatct gtgggacttt 11640 gtaagcgcgc tggagcaaaa cccaaatagc aagccgctca tggcgcagct gttccttata 11700 gtgcagcaca gcagggacaa cgaggcattc agggatgcgc tgctaaacat agtagagccc 11760 gagggccgct ggctgctcga tttgataaac atcctgcaga gcatagtggt gcaggagcgc 11820 agcttgagcc tggctgacaa ggtggccgcc atcaactatt ccatgcttag cctgggcaag 11880 ttttacgccc gcaagatata ccatacccct tacgttccca tagacaagga ggtaaagatc 11940 gaggggttct acatgcgcat ggcgctgaag gtgcttacct tgagcgacga cctgggcgtt 12000 tatcgcaacg agcgcatcca caaggccgtg agcgtgagcc ggcggcgcga gctcagcgac 12060 cgcgagctga tgcacagcct gcaaagggcc ctggctggca cgggcagcgg cgatagagag 12120 gccgagtcct actttgacgc gggcgctgac ctgcgctggg ccccaagccg acgcgccctg 12180 gaggcagctg gggccggacc tgggctggcg gtggcacccg cgcgcgctgg caacgtcggc 12240 ggcgtggagg aatatgacga ggacgatgag tacgagccag aggacggcga gtactaagcg 12300 gtgatgtttc tgatcagatg atgcaagacg caacggaccc ggcggtgcgg gcggcgctgc 12360 agagccagcc gtccggcctt aactccacgg acgactggcg ccaggtcatg gaccgcatca 12420 tgtcgctgac tgcgcgcaat cctgacgcgt tccggcagca gccgcaggcc aaccggctct 12480 ccgcaattct ggaagcggtg gtcccggcgc gcgcaaaccc cacgcacgag aaggtgctgg 12540 cgatcgtaaa cgcgctggcc gaaaacaggg ccatccggcc cgacgaggcc ggcctggtct 12600 acgacgcgct gcttcagcgc gtggctcgtt acaacagcgg caacgtgcag accaacctgg 12660 accggctggt gggggatgtg cgcgaggccg tggcgcagcg tgagcgcgcg cagcagcagg 12720 gcaacctggg ctccatggtt gcactaaacg ccttcctgag tacacagccc gccaacgtgc 12780 cgcggggaca ggaggactac accaactttg tgagcgcact gcggctaatg gtgactgaga 12840 caccgcaaag tgaggtgtac cagtctgggc cagactattt tttccagacc agtagacaag 12900 gcctgcagac cgtaaacctg agccaggctt tcaaaaactt gcaggggctg tggggggtgc 12960 gggctcccac aggcgaccgc gcgaccgtgt ctagcttgct gacgcccaac tcgcgcctgt 13020 tgctgctgct aatagcgccc ttcacggaca gtggcagcgt gtcccgggac acatacctag 13080 gtcacttgct gacactgtac cgcgaggcca taggtcaggc gcatgtggac gagcatactt 13140 tccaggagat tacaagtgtc agccgcgcgc tggggcagga ggacacgggc agcctggagg 13200 caaccctaaa ctacctgctg accaaccggc ggcagaagat cccctcgttg cacagtttaa 13260 acagcgagga ggagcgcatt ttgcgctacg tgcagcagag cgtgagcctt aacctgatgc 13320 gcgacggggt aacgcccagc gtggcgctgg acatgaccgc gcgcaacatg gaaccgggca 13380 tgtatgcctc aaaccggccg tttatcaacc gcctaatgga ctacttgcat cgcgcggccg 13440 ccgtgaaccc cgagtatttc accaatgcca tcttgaaccc gcactggcta ccgccccctg 13500 gtttctacac cgggggattc gaggtgcccg agggtaacga tggattcctc tgggacgaca 13560 tagacgacag cgtgttttcc ccgcaaccgc agaccctgct agagttgcaa cagcgcgagc 13620 aggcagaggc ggcgctgcga aaggaaagct tccgcaggcc aagcagcttg tccgatctag 13680 gcgctgcggc cccgcggtca gatgctagta gcccatttcc aagcttgata gggtctctta 13740 ccagcactcg caccacccgc ccgcgcctgc tgggcgagga ggagtaccta aacaactcgc 13800 tgctgcagcc gcagcgcgaa aaaaacctgc ctccggcatt tcccaacaac gggatagaga 13860 gcctagtgga caagatgagt agatggaaga cgtacgcgca ggagcacagg gacgtgccag 13920 gcccgcgccc gcccacccgt cgtcaaaggc acgaccgtca gcggggtctg gtgtgggagg 13980 acgatgactc ggcagacgac agcagcgtcc tggatttggg agggagtggc aacccgtttg 14040 cgcaccttcg ccccaggctg gggagaatgt tttaaaaaaa aaaaaagcat gatgcaaaat 14100 aaaaaactca ccaaggccat ggcaccgagc gttggttttc ttgtattccc cttagtatgc 14160 ggcgcgcggc gatgtatgag gaaggtcctc ctccctccta cgagagtgtg gtgagcgcgg 14220 cgccagtggc ggcggcgctg ggttctccct tcgatgctcc cctggacccg ccgtttgtgc 14280 ctccgcggta cctgcggcct accgggggga gaaacagcat ccgttactct gagttggcac 14340 ccctattcga caccacccgt gtgtacctgg tggacaacaa gtcaacggat gtggcatccc 14400 tgaactacca gaacgaccac agcaactttc tgaccacggt cattcaaaac aatgactaca 14460 gcccggggga ggcaagcaca cagaccatca atcttgacga ccggtcgcac tggggcggcg 14520 acctgaaaac catcctgcat accaacatgc caaatgtgaa cgagttcatg tttaccaata 14580 agtttaaggc gcgggtgatg gtgtcgcgct tgcctactaa ggacaatcag gtggagctga 14640 aatacgagtg ggtggagttc acgctgcccg agggcaacta ctccgagacc atgaccatag 14700 accttatgaa caacgcgatc gtggagcact acttgaaagt gggcagacag aacggggttc 14760 tggaaagcga catcggggta aagtttgaca cccgcaactt cagactgggg tttgaccccg 14820 tcactggtct tgtcatgcct ggggtatata caaacgaagc cttccatcca gacatcattt 14880 tgctgccagg atgcggggtg gacttcaccc acagccgcct gagcaacttg ttgggcatcc 14940 gcaagcggca acccttccag gagggcttta ggatcaccta cgatgatctg gagggtggta 15000 acattcccgc actgttggat gtggacgcct accaggcgag cttgaaagat gacaccgaac 15060 agggcggggg tggcgcaggc ggcagcaaca gcagtggcag cggcgcggaa gagaactcca 15120 acgcggcagc cgcggcaatg cagccggtgg aggacatgaa cgatcatgcc attcgcggcg 15180 acacctttgc cacacgggct gaggagaagc gcgctgaggc cgaagcagcg gccgaagctg 15240 ccgccccccgc tgcgcaaccc gaggtcgaga agcctcagaa gaaaccggtg atcaaacccc 15300 tgacagagga cagcaagaaa cgcagttaca acctaataag caatgacagc accttcaccc 15360 agtaccgcag ctggtacctt gcatacaact acggcgaccc tcagaccgga atccgctcat 15420 ggaccctgct ttgcactcct gacgtaacct gcggctcgga gcaggtctac tggtcgttgc 15480 cagacatgat gcaagacccc gtgaccttcc gctccacgcg ccagatcagc aactttccgg 15540 tggtgggcgc cgagctgttg cccgtgcact ccaagagctt ctacaacgac caggccgtct 15600 actcccaact catccgccag tttacctctc tgacccacgt gttcaatcgc tttcccgaga 15660 accagatttt ggcgcgcccg ccagccccca ccatcaccac cgtcagtgaa aacgttcctg 15720 ctctcacaga tcaggggacg ctaccgctgc gcaacagcat cggaggagtc cagcgagtga 15780 ccattactga cgccagacgc cgcacctgcc cctacgttta caaggccctg ggcatagtct 15840 cgccgcgcgt cctatcgagc cgcacttttt gagcaagcat gtccatcctt atatcgccca 15900 gcaataacac aggctggggc ctgcgcttcc caagcaagat gtttggcggg gccaagaagc 15960 gctccgacca acacccagtg cgcgtgcgcg ggcactaccg cgcgccctgg ggcgcgcaca 16020 aacgcggccg cactgggcgc accaccgtcg atgacgccat cgacgcggtg gtggaggagg 16080 cgcgcaacta cacgcccacg ccgccaccag tgtccacagt ggacgcggcc attcagaccg 16140 tggtgcgcgg agcccggcgc tatgctaaaa tgaagagacg gcggaggcgc gtagcacgtc 16200 gccaccgccg ccgacccggc actgccgccc aacgcgcggc ggcggccctg cttaaccgcg 16260 cacgtcgcac cggccgacgg gcggccatgc gggccgctcg aaggctggcc gcgggtattg 16320 tcactgtgcc ccccaggtcc aggcgacgag cggccgccgc agcagccgcg gccattagtg 16380 ctatgactca gggtcgcagg ggcaacgtgt attgggtgcg cgactcggtt agcggcctgc 16440 gcgtgcccgt gcgcacccgc cccccgcgca actagattgc aagaaaaaac tacttagact 16500 cgtactgttg tatgtatcca gcggcggcgg cgcgcaacga agctatgtcc aagcgcaaaa 16560 tcaaagaaga gatgctccag gtcatcgcgc cggagatcta tggccccccg aagaaggaag 16620 agcaggatta caagccccga aagctaaagc gggtcaaaaa gaaaaagaaa gatgatgatg 16680 atgaacttga cgacgaggtg gaactgctgc acgctaccgc gcccaggcga cgggtacagt 16740 ggaaaggtcg acgcgtaaaa cgtgttttgc gacccggcac caccgtagtc tttacgcccg 16800 gtgagcgctc cacccgcacc tacaagcgcg tgtatgatga ggtgtacggc gacgaggacc 16860 tgcttgagca ggccaacgag cgcctcgggg agtttgccta cggaaagcgg cataaggaca 16920 tgctggcgtt gccgctggac gagggcaacc caacacctag cctaaagccc gtaacactgc 16980 agcaggtgct gcccgcgctt gcaccgtccg aagaaaagcg cggcctaaag cgcgagtctg 17040 gtgacttggc acccaccgtg cagctgatgg tacccaagcg ccagcgactg gaagatgtct 17100 tggaaaaaat gaccgtggaa cctgggctgg agcccgaggt ccgcgtgcgg ccaatcaagc 17160 aggtggcgcc gggactgggc gtgcagaccg tggacgttca gatacccact accagtagca 17220 ccagtattgc caccgccaca gagggcatgg agacacaaac gtccccggtt gcctcagcgg 17280 tggcggatgc cgcggtgcag gcggtcgctg cggccgcgtc caagacctct acggaggtgc 17340 aaacggaccc gtggatgttt cgcgtttcag ccccccggcg cccgcgccgt tcgaggaagt 17400 acggcgccgc cagcgcgcta ctgcccgaat atgccctaca tccttccatt gcgcctaccc 17460 ccggctatcg tggctacacc taccgcccca gaagacgagc aactacccga cgccgaacca 17520 ccactggaac ccgccgccgc cgtcgccgtc gccagcccgt gctggccccg atttccgtgc 17580 gcagggtggc tcgcgaagga ggcaggaccc tggtgctgcc aacagcgcgc taccacccca 17640 gcatcgttta aaagccggtc tttgtggttc ttgcagatat ggccctcacc tgccgcctcc 17700 gtttcccggt gccgggattc cgaggaagaa tgcaccgtag gaggggcatg gccggccacg 17760 gcctgacggg cggcatgcgt cgtgcgcacc accggcggcg gcgcgcgtcg caccgtcgca 17820 tgcgcggcgg tatcctgccc ctccttattc cactgatcgc cgcggcgatt ggcgccgtgc 17880 ccggaattgc atccgtggcc ttgcaggcgc agagacactg attaaaaaca agttgcatgt 17940 ggaaaaatca aaataaaaag tctggactct cacgctcgct tggtcctgta actattttgt 18000 agaatggaag acatcaactt tgcgtctctg gccccgcgac acggctcgcg cccgttcatg 18060 ggaaactggc aagatatcgg caccagcaat atgagcggtg gcgccttcag ctggggctcg 18120 ctgtggagcg gcattaaaaa tttcggttcc accgttaaga actatggcag caaggcctgg 18180 aacagcagca caggccagat gctgagggat aagttgaaag agcaaaattt ccaacaaaag 18240 gtggtagatg gcctggcctc tggcattagc ggggtggtgg acctggccaa ccaggcagtg 18300 caaaataaga ttaacagtaa gcttgatccc cgccctcccg tagaggagcc tccaccggcc 18360 gtggagacag tgtctccaga ggggcgtggc gaaaagcgtc cgcgccccga cagggaagaa 18420 actctggtga cgcaaataga cgagcctccc tcgtacgagg aggcactaaa gcaaggcctg 18480 cccaccaccc gtcccatcgc gcccatggct accggagtgc tgggccagca cacacccgta 18540 acgctggacc tgcctccccc cgccgacacc cagcagaaac ctgtgctgcc aggcccgacc 18600 gccgttgttg taacccgtcc tagccgcgcg tccctgcgcc gcgccgccag cggtccgcga 18660 tcgttgcggc ccgtagccag tggcaactgg caaagcacac tgaacagcat cgtgggtctg 18720 ggggtgcaat ccctgaagcg ccgacgatgc ttctgatagc taacgtgtcg tatgtgtgtc 18780 atgtatgcgt ccatgtcgcc gccagaggag ctgctgagcc gccgcgcgcc cgctttccaa 18840 gatggctacc ccttcgatga tgccgcagtg gtcttacatg cacatctcgg gccaggacgc 18900 ctcggagtac ctgagccccg ggctggtgca gtttgcccgc gccaccgaga cgtacttcag 18960 cctgaataac aagtttagaa accccacggt ggcgcctacg cacgacgtga ccacagaccg 19020 gtcccagcgt ttgacgctgc ggttcatccc tgtggaccgt gaggatactg cgtactcgta 19080 caaggcgcgg ttcaccctag ctgtgggtga taaccgtgtg ctggacatgg cttccacgta 19140 ctttgacatc cgcggcgtgc tggacagggg ccctactttt aagccctact ctggcactgc 19200 ctacaacgcc ctggctccca agggtgcccc aaatccttgc gaatgggatg aagctgctac 19260 tgctcttgaa ataaacctag aagaagagga cgatgacaac gaagacgaag tagacgagca 19320 agctgagcag caaaaaactc acgtatttgg gcaggcgcct tattctggta taaatattac 19380 aaaggagggt attcaaatag gtgtcgaagg tcaaacacct aaatatgccg ataaaacatt 19440 tcaacctgaa cctcaaatag gagaatctca gtggtacgaa acagaaatta atcatgcagc 19500 tgggagagtc ctaaaaaaga ctaccccaat gaaaccatgt tacggttcat atgcaaaacc 19560 cacaaatgaa aatggagggc aaggcattct tgtaaagcaa caaaatggaa agctagaaag 1920 tcaagtggaa atgcaatttt tctcaactac tgaggcagcc gcaggcaatg gtgataactt 1980 gactcctaaa gtggtattgt acagtgaaga tgtagatata gaaaccccag acactcatat 197440 ttcttacatg cccactatta aggaaggtaa ctcacgagaa ctaatgggcc aacaatctat 1800 gcccaacagg cctaattaca ttgcttttag ggacaatttt attggtctaa tgtattacaa 19860 cagcacgggt aatatgggtg ttctggcggg ccaagcatcg cagttgaatg ctgttgtaga 19920 tttgcaagac agaaacacag agctttcata ccagcttttg cttgattcca ttggtgatag 19980 aaccaggtac ttttctatgt ggaatcaggc tgttgacagc tatgatccag atgttagaat 20040 tattgaaaat catggaactg aagatgaact tccaaattac tgctttccac tgggaggtgt 20100 gattaataca gagactctta ccaaggtaaa acctaaaaca ggtcaggaaa atggatggga 20160 aaaagatgct acagaatttt cagataaaaa tgaaataaga gttggaaata attttgccat 20220 ggaaatcaat ctaaatgcca acctgtggag aaatttcctg tactccaaca tagcgctgta 20280 tttgcccgac aagctaaagt acagtccttc caacgtaaaa atttctgata acccaaacac 20340 ctacgactac atgaacaagc gagtggtggc tcccgggcta gtggactgct acattaacct 20400 tggagcacgc tggtcccttg actatatgga caacgtcaac ccatttaacc accaccgcaa 20460 tgctggcctg cgctaccgct caatgttgct gggcaatggt cgctatgtgc ccttccacat 20520 ccaggtgcct cagaagttct ttgccattaa aaacctcctt ctcctgccgg gctcatacac 20580 ctacgagtgg aacttcagga aggatgttaa catggttctg cagagctccc taggaaatga 20640 cctaagggtt gacggagcca gcattaagtt tgatagcatt tgcctttacg ccaccttctt 20700 ccccatggcc cacaacaccg cctccacgct tgaggccatg cttagaaacg acaccaacga 20760 ccagtccttt aacgactatc tctccgccgc caacatgctc taccctatac ccgccaacgc 20820 taccaacgtg cccatatcca tcccctcccg caactgggcg gctttccgcg gctgggcctt 20880 cacgcgcctt aagactaagg aaaccccatc actgggctcg ggctacgacc cttattacac 20940 ctactctggc tctataccct acctagatgg aaccttttac ctcaaccaca cctttaagaa 21000 ggtggccatt acctttgact cttctgtcag ctggcctggc aatgaccgcc tgcttacccc 21060 caacgagttt gaaattaagc gctcagttga cggggagggt tacaacgttg cccagtgtaa 21120 catgaccaaa gactggttcc tggtacaaat gctagctaac tataacattg gctaccaggg 21180 cttctatatc ccagagagct acaaggaccg catgtactcc ttctttagaa acttccagcc 21240 catgagccgt caggtggtgg atgatactaa atacaaggac taccaacagg tgggcatcct 21300 acaccaacac aacaactctg gatttgttgg ctaccttgcc cccaccatgc gcgaaggaca 21360 ggcctaccct gctaacttcc cctatccgct tataggcaag accgcagttg acagcattac 21420 ccagaaaaag tttctttgcg atcgcaccct ttggcgcatc ccattctcca gtaactttat 21480 gtccatgggc gcactcacag acctgggcca aaaccttctc tacgccaact ccgccccacgc 21540 gctagacatg acttttgagg tggatcccat ggacgagccc acccttcttt atgttttgtt 21600 tgaagtcttt gacgtggtcc gtgtgcacca gccgcaccgc ggcgtcatcg aaaccgtgta 21660 cctgcgcacg cccttctcgg ccggcaacgc cacaacataa agaagcaagc aacatcaaca 21720 acagctgccg ccatgggctc cagtgagcag gaactgaaag ccattgtcaa agatcttggt 21780 tgtgggccat attttttggg cacctatgac aagcgctttc caggctttgt ttctccacac 21840 aagctcgcct gcgccatagt caatacggcc ggtcgcgaga ctgggggcgt acactggatg 21900 gcctttgcct ggaacccgca ctcaaaaaca tgctacctct ttgagccctt tggcttttct 21960 gaccagcgac tcaagcaggt ttaccagttt gagtacgagt cactcctgcg ccgtagcgcc 22020 attgcttctt cccccgaccg ctgtataacg ctggaaaagt ccacccaaag cgtacagggg 22080 cccaactcgg ccgcctgtgg actattctgc tgcatgtttc tccacgcctt tgccaactgg 22140 ccccaaactc ccatggatca caaccccacc atgaacctta ttaccggggt acccaactcc 22200 atgctcaaca gtccccaggt acagcccacc ctgcgtcgca accaggaaca gctctacagc 22260 ttcctggagc gccactcgcc ctacttccgc agccacagtg cgcagattag gagcgccact 22320 tctttttgtc acttgaaaaa catgtaaaaa taatgtacta gagacacttt caataaaggc 22380 aaatgctttt atttgtacac tctcgggtga ttatttaccc ccacccttgc cgtctgcgcc 22440 gtttaaaaat caaaggggtt ctgccgcgca tcgctatgcg ccactggcag ggacacgttg 22500 cgatactggt gtttagtgct ccacttaaac tcaggcacaa ccatccgcgg cagctcggtg 22560 aagttttcac tccacaggct gcgcaccatc accaacgcgt ttagcaggtc gggcgccgat 22620 atcttgaagt cgcagttggg gcctccgccc tgcgcgcgcg agttgcgata cacagggttg 22680 cagcactgga acactatcag cgccgggtgg tgcacgctgg ccagcacgct cttgtcggag 22740 atcagatccg cgtccaggtc ctccgcgttg ctcagggcga acggagtcaa ctttggtagc 22800 tgccttccca aaaagggcgc gtgcccaggc tttgagttgc actcgcaccg tagtggcatc 22860 aaaaggtgac cgtgcccggt ctgggcgtta ggatacagcg cctgcataaa agccttgatc 22920 tgcttaaaag ccacctgagc ctttgcgcct tcagagaaga acatgccgca agacttgccg 22980 gaaaactgat tggccggaca ggccgcgtcg tgcacgcagc accttgcgtc ggtgttggag 23040 atctgcacca catttcggcc ccaccggttc ttcacgatct tggccttgct agactgctcc 23100 ttcagcgcgc gctgcccgtt ttcgctcgtc acatccattt caatcacgtg ctccttattt 23160 atcataatgc ttccgtgtag acacttaagc tcgccttcga tctcagcgca gcggtgcagc 23220 cacaacgcgc agcccgtggg ctcgtgatgc ttgtaggtca cctctgcaaa cgactgcagg 23280 tacgcctgca ggaatcgccc catcatcgtc acaaaggtct tgttgctggt gaaggtcagc 23340 tgcaacccgc ggtgctcctc gttcagccag gtcttgcata cggccgccag agcttccact 23400 tggtcaggca gtagtttgaa gttcgccttt agatcgttat ccacgtggta cttgtccatc 23460 agcgcgcgcg cagcctccat gcccttctcc cacgcagaca cgatcggcac actcagcggg 23520 ttcatcaccg taatttcact ttccgcttcg ctgggctctt cctcttcctc ttgcgtccgc 23580 ataccacgcg ccactgggtc gtcttcattc agccgccgca ctgtgcgctt acctcctttg 23640 ccatgcttga ttagcaccgg tgggttgctg aaacccacca tttgtagcgc cacatcttct 23700 ctttcttcct cgctgtccac gattacctct ggtgatggcg ggcgctcggg cttgggagaa 23760 gggcgcttct ttttcttctt gggcgcaatg gccaaatccg ccgccgaggt cgatggccgc 23820 gggctgggtg tgcgcggcac cagcgcgtct tgtgatgagt cttcctcgtc ctcggactcg 23880 atacgccgcc tcatccgctt ttttgggggc gcccggggag gcggcggcga cggggacggg 23940 gacgacacgt cctccatggt tgggggacgt cgcgccgcac cgcgtccgcg ctcgggggtg 24000 gtttcgcgct gctcctcttc ccgactggcc atttccttct cctataggca gaaaaagatc 24060 atggagtcag tcgagaagaa ggacagccta accgccccct ctgagttcgc caccaccgcc 24120 tccaccgatg ccgccaacgc gcctaccacc ttccccgtcg aggcaccccc gcttgaggag 24180 gaggaagtga ttatcgagca ggacccaggt tttgtaagcg aagacgacga ggaccgctca 24240 gtaccaacag aggataaaaa gcaagaccag gacaacgcag aggcaaacga ggaacaagtc 24300 gggcgggggg acgaaaggca tggcgactac ctagatgtgg gagacgacgt gctgttgaag 24360 catctgcagc gccagtgcgc cattatctgc gacgcgttgc aagagcgcag cgatgtgccc 24420 ctcgccatag cggatgtcag ccttgcctac gaacgccacc tattctcacc gcgcgtaccc 24480 cccaaacgcc aagaaaacgg cacatgcgag cccaacccgc gcctcaactt ctaccccgta 24540 tttgccgtgc cagaggtgct tgccacctat cacatctttt tccaaaactg caagataccc 24600 ctatcctgcc gtgccaaccg cagccgagcg gacaagcagc tggccttgcg gcagggcgct 24660 gtcatacctg atatcgcctc gctcaacgaa gtgccaaaaa tctttgaggg tcttggacgc 24720 gacgagaagc gcgcggcaaa cgctctgcaa caggaaaaca gcgaaaatga aagtcactct 24780 ggagtgttgg tggaactcga gggtgacaac gcgcgcctag ccgtactaaa acgcagcatc 24840 gaggtcaccc actttgccta cccggcactt aacctacccc ccaaggtcat gagcacagtc 24900 atgagtgagc tgatcgtgcg ccgtgcgcag cccctggaga gggatgcaaa tttgcaagaa 24960 caaacagagg agggcctacc cgcagttggc gacgagcagc tagcgcgctg gcttcaaacg 25020 cgcgagcctg ccgacttgga ggagcgacgc aaactaatga tggccgcagt gctcgttacc 25080 gtggagcttg agtgcatgca gcggttcttt gctgacccgg agatgcagcg caagctagag 25140 gaaacattgc actacacctt tcgacagggc tacgtacgcc aggcctgcaa gatctccaac 25200 gtggagctct gcaacctggt ctcctacctt ggaattttgc acgaaaaccg ccttgggcaa 25260 aacgtgcttc attccacgct caagggcgag gcgcgccgcg actacgtccg cgactgcgtt 25320 tacttatttc tatgctacac ctggcagacg gccatgggcg tttggcagca gtgcttggag 25380 gagtgcaacc tcaaggagct gcagaaactg ctaaagcaaa acttgaagga cctatggacg 25440 gccttcaacg agcgctccgt ggccgcgcac ctggcggaca tcattttccc cgaacgcctg 25500 cttaaaaccc tgcaacaggg tctgccagac ttcaccagtc aaagcatgtt gcagaacttt 25560 aggaacttta tcctagagcg ctcaggaatc ttgcccgcca cctgctgtgc acttcctagc 25620 gactttgtgc ccattaagta ccgcgaatgc cctccgccgc tttggggcca ctgctacctt 25680 ctgcagctag ccaactacct tgcctaccac tctgacataa tggaagacgt gagcggtgac 25740 ggtctactgg agtgtcactg tcgctgcaac ctatgcaccc cgcaccgctc cctggtttgc 25800 aattcgcagc tgcttaacga aagtcaaatt atcggtacct ttgagctgca gggtccctcg 25860 cctgacgaaa agtccgcggc tccggggttg aaactcactc cggggctgtg gacgtcggct 25920 taccttcgca aatttgtacc tgaggactac cacgcccacg agattaggtt ctacgaagac 25980 caatcccgcc cgcctaatgc ggagcttacc gcctgcgtca ttacccaggg ccacattctt 26040 ggccaattgc aagccatcaa caaagcccgc caagagtttc tgctacgaaa gggacggggg 26100 gtttacttgg acccccagtc cggcgaggag ctcaacccaa tccccccgcc gccgcagccc 26160 tatcagcagc agccgcgggc ccttgcttcc caggatggca cccaaaaaga agctgcagct 26220 gccgccgcca cccacggacg aggaggaata ctgggacagt caggcagagg aggttttgga 26280 cgaggaggag gaggacatga tggaagactg ggagagccta gacgaggaag cttccgaggt 26340 cgaagaggtg tcagacgaaa caccgtcacc ctcggtcgca ttcccctcgc cggcgcccca 26400 gaaatcggca accggttcca gcatggctac aacctccgct cctcaggcgc cgccggcact 26460 gcccgttcgc cgacccaacc gtagatggga caccactgga accagggccg gtaagtccaa 26520 gcagccgccg ccgttagccc aagagcaaca acagcgccaa ggctaccgct catggcgcgg 26580 gcacaagaac gccatagttg cttgcttgca agactgtggg ggcaacatct ccttcgcccg 26640 ccgctttctt ctctaccatc acggcgtggc cttcccccgt aacatcctgc attactaccg 26700 tcatctctac agcccatact gcaccggcgg cagcggcagc aacagcagcg gccacacaga 26760 agcaaaggcg accggatagc aagactctga caaagcccaa gaaatccaca gcggcggcag 26820 cagcaggagg aggagcgctg cgtctggcgc ccaacgaacc cgtatcgacc cgcgagctta 26880 gaaacaggat ttttcccact ctgtatgcta tatttcaaca gagcaggggc caagaacaag 26940 agctgaaaat aaaaaacagg tctctgcgat ccctcacccg cagctgcctg tatcacaaaa 27000 gcgaagatca gcttcggcgc acgctggaag acgcggaggc tctcttcagt aaatactgcg 27060 cgctgactct taaggactag tttcgcgccc tttctcaaat ttaagcgcga aaactacgtc 27120 atctccagcg gccacacccg gcgccagcac ctgttgtcag cgccattatg agcaaggaaa 27180 ttccccacgcc ctacatgtgg agttaccagc cacaaatggg acttgcggct ggagctgccc 27240 aagactactc aacccgaata aactacatga gcgcgggacc ccacatgata tcccgggtca 27300 acggaatacg cgcccaccga aaccgaattc tcctggaaca ggcggctatt accaccacac 27360 ctcgtaataa ccttaatccc cgtagttggc ccgctgccct ggtgtaccag gaaagtcccg 27420 ctcccaccac tgtggtactt cccagagacg cccaggccga agttcagatg actaactcag 27480 gggcgcagct tgcgggcggc tttcgtcaca gggtgcggtc gcccgggcag ggtataactc 27540 acctgacaat cagagggcga ggtattcagc tcaacgacga gtcggtgagc tcctcgcttg 27600 gtctccgtcc ggacgggaca tttcagatcg gcggcgccgg ccgctcttca ttcacgcctc 27660 gtcaggcaat cctaactctg cagacctcgt cctctgagcc gcgctctgga ggcattggaa 27720 ctctgcaatt tattgaggag tttgtgccat cggtctactt taaccccttc tcgggacctc 27780 ccggccacta tccggatcaa tttattccta actttgacgc ggtaaaggac tcggcggacg 27840 gctacgactg aatgttaagt ggagaggcag agcaactgcg cctgaaacac ctggtccact 27900 gtcgccgcca caagtgcttt gcccgcgact ccggtgagtt ttgctacttt gaattgcccg 27960 aggatcatat cgagggcccg gcgcacggcg tccggcttac cgcccaggga gagcttgccc 28020 gtagcctgat tcgggagttt acccagcgcc ccctgctagt tgagcgggac aggggaccct 28080 gtgttctcac tgtgatttgc aactgtccta accctggatt acatcaagat ctttgttgcc 28140 atctctgtgc tgagtataat aaatacagaa attaaaatat actggggctc ctatcgccat 28200 cctgtaaacg ccaccgtctt cacccgccca agcaaaccaa ggcgaacctt acctggtact 28260 tttaacatct ctccctctgt gatttacaac agtttcaacc cagacggagt gagtctacga 28320 gagaacctct ccgagctcag ctactccatc agaaaaaaca ccaccctcct tacctgccgg 28380 gaacgtacga gtgcgtcacc ggccgctgca ccacacctac cgcctgaccg taaaccagac 28440 tttttccgga cagacctcaa taactctgtt taccagaaca ggaggtgagc ttagaaaacc 28500 cttagggtat taggccaaag gcgcagctac tgtggggttt atgaacaatt caagcaactc 28560 tacgggctat tctaattcag gtttctctag aatcggggtt ggggttattc tctgtcttgt 28620 gattctcttt attcttatac taacgcttct ctgcctaagg ctcgccgcct gctgtgtgca 28680 catttgcatt tattgtcagc tttttaaacg ctggggtcgc cacccaagat gattaggtac 28740 ataatcctag gtttactcac ccttgcgtca gcccacggta ccacccaaaa ggtggatttt 28800 aaggagccag cctgtaatgt tacattcgca gctgaagcta atgagtgcac cactcttata 28860 aaatgcacca cagaacatga aaagctgctt attcgccaca aaaacaaaat tggcaagtat 28920 gctgtttatg ctatttggca gccaggtgac actacagagt ataatgttac agttttccag 28980 ggtaaaagtc ataaaacttt tatgtatact tttccatttt atgaaatgtg cgacattacc 29040 atgtacatga gcaaacagta taagttgtgg cccccacaaa attgtgtgga aaacactggc 29100 actttctgct gcactgctat gctaattaca gtgctcgctt tggtctgtac cctactctat 29160 attaaataca aaagcagacg cagctttatt gaggaaaaga aaatgcctta atttactaag 29220 ttacaaagct aatgtcacca ctaactgctt tactcgctgc ttgcaaaaca aattcaaaaa 29280 gttagcatta taattagaat aggatttaaa ccccccggtc atttcctgct caataccatt 29340 cccctgaaca attgactcta tgtgggatat gctccagcgc tacaaccttg aagtcaggct 29400 tcctggatgt cagcatctga ctttggccag cacctgtccc gcggatttgt tccagtccaa 29460 ctacagcgac ccaccctaac agagatgacc aacacaacca acgcggccgc cgctaccgga 29520 cttacatcta ccacaaatac accccaagtt tctgcctttg tcaataactg ggataacttg 29580 ggcatgtggt ggttctccat agcgcttatg tttgtatgcc ttattattat gtggctcatc 29640 tgctgcctaa agcgcaaacg cgcccgacca cccatctata gtcccatcat tgtgctacac 29700 ccaaacaatg atggaatcca tagattggac ggactgaaac acatgttctt ttctcttaca 29760 gtatgattaa atgagacatg attcctcgag tttttatatt actgaccctt gttgcgcttt 29820 ttttgtgcgt gctccacatt ggctgcggtt tctcacatcg aagtagactg cattccagcc 29880 ttcacagtct atttgcttta cggatttgtc accctcacgc tcatctgcag cctcatcact 29940 gtggtcatcg cctttatcca gtgcattgac tgggtctgtg tgcgctttgc atatctcaga 30000 caccatcccc agtacaggga caggactata gctgagcttc ttagaattct ttaattatga 30060 aatttactgt gacttttctg ctgattattt gcaccctatc tgcgttttgt tccccgacct 30120 ccaagcctca aagacatata tcatgcagat tcactcgtat atggaatatt ccaagttgct 30180 acaatgaaaa aagcgatctt tccgaagcct ggttatatgc aatcatctct gttatggtgt 30240 tctgcagtac catcttagcc ctagctatat atccctacct tgacattggc tggaacgcaa 30300 tagatgccat gaaccaccca actttccccg cgcccgctat gcttccactg caacaagttg 30360 ttgccggcgg ctttgtccca gccaatcagc ctcgcccacc ttctcccacc cccactgaaa 30420 tcagctactt taatctaaca ggaggagatg actgacaccc tagatctaga aatggacgga 30480 attattacag agcagcgcct gctagaaaga cgcagggcag cggccgagca acagcgcatg 30540 aatcaagagc tccaagacat ggttaacttg caccagtgca aaaggggtat cttttgtctg 30600 gtaaagcagg ccaaagtcac ctacgacagt aataccaccg gacaccgcct tagctacaag 30660 ttgccaacca agcgtcagaa attggtggtc atggtgggag aaaagcccat taccataact 30720 cagcactcgg tagaaaccga aggctgcatt cactcacctt gtcaaggacc tgaggatctc 30780 tgcaccctta ttaagaccct gtgcggtctc aaagatctta ttccctttaa ctaataaaaa 30840 aaaataataa agcatcactt acttaaaatc agttagcaaa tttctgtcca gtttattcag 30900 cagcacctcc ttgccctcct cccagctctg gtattgcagc ttcctcctgg ctgcaaactt 30960 tctccacaat ctaaatggaa tgtcagtttc ctcctgttcc tgtccatccg cacccactat 31020 cttcatgttg ttgcagatga agcgcgcaag accgtctgaa gataccttca accccgtgta 31080 tccatatgac acggaaaccg gtcctccaac tgtgcctttt cttactcctc cctttgtatc 31140 ccccaatggg tttcaagaga gtccccctgg ggtactctct ttgcgcctat ccgaacctct 31200 agttacctcc aatggcatgc ttgcgctcaa aatgggcaac ggcctctctc tggacgaggc 31260 cggcaacctt acctcccaaa atgtaaccac tgtgagccca cctctcaaaa aaaccaagtc 31320 aaacataaac ctggaaatat ctgcacccct cacagttacc tcagaagccc taactgtggc 31380 tgccgccgca cctctaatgg tcgcgggcaa cacactcacc atgcaatcac aggccccgct 31440 aaccgtgcac gactccaaac ttagcattgc cacccaagga cccctcacag tgtcagaagg 31500 aaagctagcc ctgcaaacat caggccccct caccaccacc gatagcagta cccttactat 31560 cactgcctca ccccctctaa ctactgccac tggtagcttg ggcattgact tgaaagagcc 31620 catttataca caaaatggaa aactaggact aaagtacggg gctcctttgc atgtaacaga 31680 cgacctaaac actttgaccg tagcaactgg tccaggtgtg actattaata atacttcctt 31740 gcaaactaaa gttactggag ccttgggttt tgattcacaa ggcaatatgc aacttaatgt 31800 agcaggagga ctaaggattg attctcaaaa cagacgcctt atacttgatg ttagttatcc 31860 gtttgatgct caaaaccaac taaatctaag actaggacag ggccctcttt ttataaactc 31920 agcccacaac ttggatatta actacaacaa aggcctttac ttgtttacag cttcaaacaa 31980 ttccaaaaag cttgaggtta acctaagcac tgccaagggg ttgatgtttg acgctacagc 32040 catagccatt aatgcaggag atgggcttga atttggttca cctaatgcac caaacacaaa 32100 tcccctcaaa acaaaaattg gccatggcct agaatttgat tcaaacaagg ctatggttcc 32160 taaactagga actggcctta gttttgacag cacaggtgcc attacagtag gaaacaaaaa 32220 taatgataag ctaactttgt ggaccacacc agctccatct cctaactgta gactaaatgc 32280 agagaaagat gctaaactca ctttggtctt aacaaaatgt ggcagtcaaa tacttgctac 32340 agtttcagtt ttggctgtta aaggcagttt ggctccaata tctggaacag ttcaaagtgc 32400 tcatcttatt ataagatttg acgaaaatgg agtgctacta aacaattcct tcctggaccc 32460 agaatattgg aactttagaa atggagatct tactgaaggc acagcctata caaacgctgt 32520 tggatttatg cctaacctat cagcttatcc aaaatctcac ggtaaaactg ccaaaagtaa 32580 cattgtcagt caagtttact taaacggaga caaaactaaa cctgtaacac taaccattac 32640 actaaacggt acacaggaaa caggagacac aactccaagt gcatactcta tgtcattttc 32700 atgggactgg tctggccaca actacattaa tgaaatattt gccacatcct cttacacttt 32760 ttcatacatt gcccaagaat aaagaatcgt ttgtgttatg tttcaacgtg tttatttttc 32820 aattgcagaa aatttcaagt catttttcat tcagtagtat agccccacca ccacatagct 32880 tatacagatc accgtacctt aatcaaactc acagaaccct agtattcaac ctgccacctc 32940 cctcccaaca cacagagtac acagtccttt ctccccggct ggccttaaaa agcatcatat 33000 catgggtaac agacatattc ttaggtgtta tattccacac ggtttcctgt cgagccaaac 33060 gctcatcagt gatattaata aactccccgg gcagctcact taagttcatg tcgctgtcca 33120 gctgctgagc cacaggctgc tgtccaactt gcggttgctt aacgggcggc gaaggagaag 33180 tccacgccta catgggggta gagtcataat cgtgcatcag gatagggcgg tggtgctgca 33240 gcagcgcgcg aataaactgc tgccgccgcc gctccgtcct gcaggaatac aacatggcag 33300 tggtctcctc agcgatgatt cgcaccgccc gcagcataag gcgccttgtc ctccgggcac 33360 agcagcgcac cctgatctca cttaaatcag cacagtaact gcagcacagc accacaatat 33420 tgttcaaaat cccacagtgc aaggcgctgt atccaaagct catggcgggg accacagaac 33480 ccacgtggcc atcataccac aagcgcaggt agattaagtg gcgacccctc ataaacacgc 33540 tggacataaa cattacctct tttggcatgt tgtaattcac cacctcccgg taccatataa 33600 acctctgatt aaacatggcg ccatccacca ccatcctaaa ccagctggcc aaaacctgcc 33660 cgccggctat acactgcagg gaaccgggac tggaacaatg acagtggaga gcccaggact 33720 cgtaaccatg gatcatcatg ctcgtcatga tatcaatgtt ggcacaacac aggcacacgt 33780 gcatacactt cctcaggatt acaagctcct cccgcgttag aaccatatcc cagggaacaa 33840 cccattcctg aatcagcgta aatcccacac tgcagggaag acctcgcacg taactcacgt 33900 tgtgcattgt caaagtgtta cattcgggca gcagcggatg atcctccagt atggtagcgc 33960 gggtttctgt ctcaaaagga ggtagacgat ccctactgta cggagtgcgc cgagacaacc 34020 gagatcgtgt tggtcgtagt gtcatgccaa atggaacgcc ggacgtagtc atatttcctg 34080 aagcaaaacc aggtgcgggc gtgacaaaca gatctgcgtc tccggtctcg ccgcttagat 34140 cgctctgtgt agtagttgta gtatatccac tctctcaaag catccaggcg ccccctggct 34200 tcgggttcta tgtaaactcc ttcatgcgcc gctgccctga taacatccac caccgcagaa 34260 taagccacac ccagccaacc tacacattcg ttctgcgagt cacacacggg aggagcggga 34320 agagctggaa gaaccatgtt tttttttttt attccaaaag attatccaaa acctcaaaat 34380 gaagatctat taagtgaacg cgctcccctc cggtggcgtg gtcaaactct acagccaaag 34440 aacagataat ggcatttgta agatgttgca caatggcttc caaaaggcaa acggccctca 34500 cgtccaagtg gacgtaaagg ctaaaccctt cagggtgaat ctcctctata aacattccag 34560 caccttcaac catgcccaaa taattctcat ctcgccacct tctcaatata tctctaagca 34620 aatcccgaat attaagtccg gccattgtaa aaatctgctc cagagcgccc tccaccttca 34680 gcctcaagca gcgaatcatg attgcaaaaa ttcaggttcc tcacagacct gtataagatt 34740 caaaagcgga acattaacaa aaataccgcg atcccgtagg tcccttcgca gggccagctg 34800 aacataatcg tgcaggtctg cacggaccag cgcggccact tccccgccag gaaccatgac 34860 aaaagaaccc acactgatta tgacacgcat actcggagct atgctaacca gcgtagcccc 34920 gatgtaagct tgttgcatgg gcggcgatat aaaatgcaag gtgctgctca aaaaatcagg 34980 caaagcctcg cgcaaaaaag aaagcacatc gtagtcatgc tcatgcagat aaaggcaggt 35040 aagctccgga accaccacag aaaaagacac catttttctc tcaaacatgt ctgcgggttt 35100 ctgcataaac acaaaataaa ataacaaaaa aacatttaaa cattagaagc ctgtcttaca 35160 acaggaaaaa caacccttat aagcataaga cggactacgg ccatgccggc gtgaccgtaa 35220 aaaaactggt caccgtgatt aaaaagcacc accgacagct cctcggtcat gtccggagtc 35280 ataatgtaag actcggtaaa cacatcaggt tgattcacat cggtcagtgc taaaaagcga 35340 ccgaaatagc ccgggggaat acatacccgc aggcgtagag acaacattac agcccccata 35400 ggaggtataa caaaattaat aggagagaaa aacacataaa cacctgaaaa accctcctgc 35460 ctaggcaaaa tagcaccctc ccgctccaga acaacataca gcgcttccac agcggcagcc 35520 ataacagtca gccttaccag taaaaaagaa aacctattaa aaaaacacca ctcgacacgg 35580 caccagctca atcagtcaca gtgtaaaaaa gggccaagtg cagagcgagt atatatagga 35640 ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc cagaaaaccg cacgcgaacc 35700 tacgcccaga aacgaaagcc aaaaaaccca caacttcctc aaatcgtcac ttccgttttc 35760 ccacgttacg tcacttccca ttttaagaaa actacaattc ccaacacata caagttactc 35820 cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc gcgccacgtc acaaactcca 35880 ccccctcatt atcatattgg cttcaatcca aaataaggta tattattgat gatg 35934 <210> 2 <211> 3171 <212> DNA <213> Homo sapiens <400> 2 atggacagct cccatctgcg cgatgttgtc atcaagctcc gaccgccgcg cgcggacatc 60 tggactttgg gctcgcgcgg cgtggtggtc cgatccaccg taactcccct cgagcagcca 120 gacggtcaag gacaagcagc cgaagtagaa gaccaccagc caaacccgcc aggcgagggg 180 ctcaaattcc cactctgctt ccttgtgcgc ggtcgtcagg tcaacctcgt gcaggatgta 240 cagcccgtgc accgctgcca gtactgcgca cgtttttaca aaagccagca cgagtgttcg 300 gcccgtcgca gggacttcta ctttcaccac atcaatagcc actcctccaa ttggtggcgg 360 gagatccagt tcttcccgat cggctcgcat cctcgcaccg agcgtctctt tgtcacctac 420 gatgtagaga cctatacttg gatgggggcc tttgggaagc agctcgtgcc cttcatgctg 480 gtcatgaagt tcggcggaga tgagcctcta gtgactgccg cgcgagacct agccgcgaac 540 cttggatggg accgctggga acaagacccg cttaccttct actgcatcac cccagaaaaa 600 atggccatag gtcgccagtt taggaccttt cgcgaccacc tgcaaatgct aatggcccgt 660 gacctgtgga gctcattcgt cgcttccaac cctcatcttg cagactgggc cctttcagag 720 cacgggctca gctcccctga agagctcacc tacgaggaac ttaaaaaatt gccttccatc 780 aagggcatcc cgcgcttctt ggaactttac attgtgggcc acaacatcaa cggctttgac 840 gagatcgtgc tcgccgccca ggtaattaac aaccgttccg aggtgccggg acccttccgc 900 atcacacgca actttatgcc tcgcgcggga aagatactct tcaacgatgt caccttcgcc 960 ctgccaaatc cgcgttccaa aaagcgcacg gactttttgc tctgggagca gggcggatgc 1020 gacgacactg acttcaaata ccagtacctc aaagtcatgg tcagggacac ctttgcgctc 1080 acccacacct cgctccggaa ggccgcgcag gcatacgcgc tacccgtaga aaagggatgc 1140 tgcgcctacc aggccgtcaa ccagttctac atgctaggct cttaccgttc ggaggccgac 1200 gggtttccga tccaagagta ctggaaagac cgcgaagagt ttgtcctcaa ccgcgagctg 1260 tggaaaaaaa agggacagga taagtatgac atcatcaagg aaaccctgga ctactgcgcc 1320 ctagacgtgc aggtcaccgc cgagctggtc aacaagctgc gcgactccta cgcctccttc 1380 gtgcgtgacg cggtaggtct cacagacgcc agcttcaacg tcttccagcg tccaaccata 1440 tcatccaact cacatgccat cttcaggcag atagtcttcc gagcagagca gcccgcccgt 1500 agcaacctcg gtcccgacct cctcgctccc tcgcacgaac tatacgatta cgtgcgcgcc 1560 agcatccgcg gtggaagatg ctaccctaca tatcttggaa tactcagaga gcccctctac 1620 gtttacgaca tttgcggcat gtacgcctcc gcgctcaccc accccatgcc atggggtccc 1680 ccactcaacc catacgagcg cgcgcttgcc gcccgcgcat ggcagcaggc gctagacttg 1740 caaggatgca agatagacta cttcgacgcg cgcctgctgc ccggggtctt taccgtggac 1800 gcagaccccc cggacgagac gcagctagac cccctaccgc cattctgctc gcgcaagggc 1860 ggccgcctct gctggaccaa cgagcgccta cgcggagagg tagccaccag cgttgacctt 1920 gtcaccctgc acaaccgcgg ttggcgcgtg cacctggtgc ccgacgagcg caccaccgtc 1980 tttcccgaat ggcggtgcgt tgcgcgcgaa tacgtgcagc taaacatcgc ggccaaggag 2040 cgcgccgatc gcgacaaaaa ccaaaccctg cgctccatcg ccaagttgct gtccaacgcc 2100 ctctacgggt cgtttgccac caagcttgac aacaaaaaga ttgtcttttc tgaccagatg 2160 gatgcggcca ccctcaaagg catcaccgcg ggccaggtga atatcaaatc ctcctcgttt 2220 ttggaaactg acaatcttag cgcagaagtc atgcccgctt ttcagaggga gtactcaccc 2280 caacagctgg ccctcgcaga cagcgatgcg gaagagagtg aggacgaacg cgccccccacc 2340 cccttttata gccccccttc aggaacaccc ggtcacgtgg cctacaccta caaaccaatc 2400 accttccttg atgccgaaga gggcgacatg tgtcttcaca ccctggagcg agtggacccc 2460 ctagtggaca acgaccgcta cccctcccac ttagcctcct tcgtgctggc ctggacgcga 2520 gcctttgtct cagagtggtc cgagtttcta tacgaggagg accgcggaac accgctcgag 2580 gacaggcctc tcaagtctgt atacggggac acggacagcc ttttcgtcac cgagcgtgga 2640 caccggctca tggaaaccag aggtaagaaa cgcatcaaaa agcatggggg aaacctggtt 2700 tttgaccccg aacggccaga gctcacctgg ctcgtggaat gcgagaccgt ctgcggggcc 2760 tgcggcgcgg atgcctactc cccggaatcg gtatttctcg cgcccaagct ctacgccctc 2820 aaaagtctgc actgcccctc gtgcggcgcc tcctccaagg gcaagctgcg cgccaagggc 2880 cacgccgcgg aggggctgga ctatgacacc atggtcaaat gctacctggc cgacgcgcag 2940 ggcgaagacc ggcagcgctt cagcaccagc aggaccagcc tcaagcgcac cctggccagc 3000 gcgcagcccg gagcgcaccc cttcaccgtg acccagacta cgctgacgag gaccctgcgc 3060 ccgtggaaag acatgaccct ggcccgtctg gacgagcacc gactactgcc gtacagcgaa 3120 agccgcccca acccgcgaaa cgaggagata tgctggatcg agatgccgta g 3171 <210> 3 <211> 1962 <212> DNA <213> Homo sapiens <400> 3 atggagcact ttttgccgct gcgcaacatc tggaaccgcg tccgcgactt tccgcgcgcc 60 tccaccaccg ccgccggcat cacctggatg tccaggtaca tctacggata tcatcgcctt 120 atgttggaag atctcgcccc cggagccccg gccaccctac gctggcccct ctaccgccag 180 ccgccgccgc actttttggt gggataccag tacctggtgc ggacttgcaa cgactacgta 240 tttgactcga gggcttactc gcgtctcagg tacaccgagc tctcgcagcc gggtcaccag 300 accgttaact ggtccgttat ggccaactgc acttacacca tcaacacggg cgcataccac 360 cgctttgtgg acatggatga cttccagtct accctcacgc aggtgcagca ggccatatta 420 gccgagcgcg ttgtcgccga cctagccctg cttcagccga tgaggggctt cggggtcaca 480 cgcatgggag gaagagggcg ccacctacgg ccaaactccg ccgccgccgc agcgatagat 540 gcaagagatg caggacaaga ggaaggagaa gaagaagtgc cggtagaaag gctcatgcaa 600 gactactaca aagacctgcg ccgatgtcaa aacgaagcct ggggcatggc cgaccgcctg 660 cgcattcagc aggccggacc caaggacatg gtgcttctgt cgaccatccg ccgtctcaag 720 accgcctact ttaattacat catcagcagc acctccgcca gaaacaaccc cgaccgccgc 780 ccgctgccgc ccgccacggt gctcagccta ccttgcgact gtgactggtt agacgccttt 840 ctcgagaggt tttccgatcc ggtcgatgcg gactcgctca ggtccctcgg cggcggagta 900 cctacacaac aattgttgag atgcatcgtt agcgccgtat ccctgccgca tggcagcccc 960 ccgccaaccc ataaccggga catgacgggc ggcgtcttcc aactgcgccc ccgcgagaac 1020 ggccgcgccg tcaccgagac catgcgccgt cgccgcgggg agatgatcga gcgctttgtc 1080 gaccgcctcc cggtgcgccg tcgtcgccgc cgtgtccccc ctccccccacc gccgccagaa 1140 gaagaagaag gggaggccct tatggaagag gagattgaag aagaagaaga ggcccctgta 1200 gcctttgagc gcgaggtgcg cgacactgtc gccgagctca tccgtcttct ggaggaggag 1260 ttaaccgtgt cggcgcgcaa ctcccagttt ttcaacttcg ccgtggactt ctacgaggcc 1320 atggagcgcc ttgaggcctt gggggatatc aacgaatcca cgttgcgacg ctgggttatg 1380 tacttcttcg tggcagaaca caccgccacc accctcaact acctctttca gcgcctgcga 1440 aactacgccg tcttcgcccg gcacgtggag ctcaatctcg cgcaggtggt catgcgcgcc 1500 cgcgatgccg aagggggcgt ggtctacagc cgcgtctgga acgagggagg cctcaacgcc 1560 ttctcgcagc tcatggcccg catttccaac gacctcgccg ccaccgtgga gcgagccgga 1620 cgcggagatc tccaggagga agagatcgag cagttcatgg ccgagatcgc ctatcaagac 1680 aactcaggag acgtgcagga gattttgcgc caggccgccg tcaacgacac cgaaattgat 1740 tctgtcgaac tctctttcag gttcaagctc accgggcccg tcgtcttcac gcagaggcgc 1800 cagattcagg agatcaaccg ccgcgtcgtc gcgttcgcca gcaacctacg cgcgcagcac 1860 cagctcctgc ccgcgcgcgg cgccgacgtg cccctgcccc ctctcccggc gggtccggag 1920 ccccccctac ctccgggggc tcgcccgcgt caccgctttt ag 1962 <210> 4 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 4 cccgtataca gacttgagag gcctgtcctc gagc 34 <210> 5 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 5 gccgtttaaa catcgatacc gttcggaggc cgacg 35 <210> 6 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 6 gcattcgaaa ctccgccgcc gagggacctg 30 <210> 7 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 7 gcacatatgg tttaaacgaa aagcaaaaaa ggggc 35 <210> 8 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 8 gtccgtgtcc ccgtatacag acttgagagg cctgtcct 38 <210> 9 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 9 ctattgaatt cgtttaaacg aaaagcaaaa aaggggc 37 <210> 10 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 10 gcggcggagt atcgataccg ttcggaggc 29 <210> 11 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 11 gcctccgaac ggtatcgata ctccgccgc 29 <210> 12 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 12 atgaagggca cgagctgctt 20 <210> 13 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 13 atcaacgaat ccacgttgcg ac 22 <210> 14 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 14 gcattaatcg atatgaaggg cacgagctgc tt 32 <210> 15 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 15 gcattaatcg atacctacat caacgaatcc acgttgcgac 40 <210> 16 <211> 6 <212> DNA <213> Artificial Sequence <220> <223> ClaI restriction enzyme site <400> 16 gtaggt 6 <210> 17 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 17 gtggattcgt tgatgtaggt actccgccgc c 31 <210> 18 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 18 ggcggcggag tacctacat aacgaatcca c 31 <210> 19 <211> 4145 <212> DNA <213> Artificial Sequence <220> <223> preparation example2 <400> 19 gtatacagac ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct cgtatagaaa 60 ctcggaccac tctgagacaa aggctcgcgt ccaggccagc acgaaggagg ctaagtggga 120 ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa gacacatgtc 180 gccctcttcg gcatcaagga aggtgattgg tttgtaggtg taggccacgt gaccgggtgt 240 tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct cttccgcatc 300 gctgtctgcg agggccagct gttggggtga gtactccctc tgaaaagcgg gcatgacttc 360 tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt 420 gatgcctttg agggtggccg catccatctg gtcagaaaag acaatctttt tgttgtcaag 480 cttggtggca aacgacccgt agagggcgtt ggacagcaac ttggcgatgg agcgcagggt 540 ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca cgtattcgcg 600 cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca ggtgcacgcg 660 ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc cgcgtaggcg 720 ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag aatggcggta gggggtctag 780 ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc ccgggcagca ggcgcgcgtc 840 gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc gggcggcaag 900 cgcgcgctcg tatgggttga gtgggggacc ccatggcatg gggtgggtga gcgcggaggc 960 gtacatgccg caaatgtcgt aaacgtagag gggctctctg agtattccaa gatatgtagg 1020 gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt gcgagggagc 1080 gaggaggtcg ggaccgaggt tgctacgggc gggctgctct gctcggaaga ctatctgcct 1140 gaagatggca tgtgagttgg atgatatggt tggacgctgg aagacgttga agctggcgtc 1200 tgtgagacct accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct tgttgaccag 1260 ctcggcggtg acctgcacgt ctagggcgca gtagtccagg gtttccttga tgatgtcata 1320 cttatcctgt cccttttttt tccacagctc gcggttgagg acaaactctt cgcggtcttt 1380 ccagtactct tggatcggaa acccgtcggc ctccgaacgg tatcgatatg aagggcacga 1440 gctgcttccc aaaggccccc atccaagtat aggtctctac atcgtaggtg acaaagagac 1500 gctcggtgcg aggatgcgag ccgatcggga agaactggat ctcccgccac caattggagg 1560 agtggctatt gatgtggtga aagtagaagt ccctgcgacg ggccgaacac tcgtgctggc 1620 ttttgtaaaa acgtgcgcag tactggcagc ggtgcacggg ctgtacatcc tgcacgaggt 1680 tgacctgacg accgcgcaca aggaagcaga gtgggaattt gagcccctcg cctggcgggt 1740 ttggctggtg gtcttctact tcggctgctt gtccttgacc gtctggctgc tcgaggggag 1800 ttacggtgga tcggaccacc acgccgcgcg agcctaaagt ccagatgtcc gcgcgcggcg 1860 gtcggagctt gatgacaaca tcgcgcagat gggagctgtc catggtctgg agctcccgcg 1920 gcgtcaggtc aggcgggagc tcctgcaggt ttacctcgca tagacgggtc agggcgcggg 1980 ctagatccag gtgataccta atttccaggg gctggttggt ggcggcgtcg atggcttgca 2040 agaggccgca tccccgcggc gcgactacgg taccgcgcgg cgggcggtgg gccgcggggg 2100 tgtccttgga tgatgcatct aaaagcggtg acgcgggcga gcccccggag gtaggggggg 2160 ctccggaccc gccgggagag ggggcagggg cacgtcggcg ccgcgcgcgg gcaggagctg 2220 gtgctgcgcg cgtaggttgc tggcgaacgc gacgacgcgg cggttgatct cctgaatctg 2280 gcgcctctgc gtgaagacga cgggcccggt gagcttgaac ctgaaagaga gttcgacaga 2340 atcaatttcg gtgtcgttga cggcggcctg gcgcaaaatc tcctgcacgt ctcctgagtt 2400 gtcttgatag gcgatctcgg ccatgaactg ctcgatctct tcctcctgga gatctccgcg 2460 tccggctcgc tccacggtgg cggcgaggtc gttggaaatg cgggccatga gctgcgagaa 2520 ggcgttgagg cctccctcgt tccagacgcg gctgtagacc acgccccctt cggcatcgcg 2580 ggcgcgcatg accacctgcg cgagattgag ctccacgtgc cgggcgaaga cggcgtagtt 2640 tcgcaggcgc tgaaagaggt agttgagggt ggtggcggtg tgttctgcca cgaagaagta 2700 cataacccag cgtcgcaacg tggattcgtt gatgtaggta ctccgccgcc gagggacctg 2760 agcgagtccg catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag 2820 tcgcaaggta ggctgagcac cgtggcgggc ggcagcgggc ggcggtcggg gttgtttctg 2880 gcggaggtgc tgctgatgat gtaattaaag taggcggtct tgagacggcg gatggtcgac 2940 agaagcacca tgtccttggg tccggcctgc tgaatgcgca ggcggtcggc catgccccag 3000 gcttcgtttt gacatcggcg caggtctttg tagtagtctt gcatgagcct ttctaccggc 3060 acttcttctt ctccttcctc ttgtcctgca tctcttgcat ctatcgctgc ggcggcggcg 3120 gagtttggcc gtaggtggcg ccctcttcct cccatgcgtg tgaccccgaa gcccctcatc 3180 ggctgaagca gggctaggtc ggcgacaacg cgctcggcta atatggcctg ctgcacctgc 3240 gtgagggtag actggaagtc atccatgtcc acaaagcggt ggtatgcgcc cgtgttgatg 3300 gtgtaagtgc agttggccat aacggaccag ttaacggtct ggtgacccgg ctgcgagagc 3360 tcggtgtacc tgagacgcga gtaagccctc gagtcaaata cgtagtcgtt gcaagtccgc 3420 accaggtact ggtatcccac caaaaagtgc ggcggcggct ggcggtagag gggccagcgt 3480 agggtggccg gggctccggg ggcgagatct tccaacataa ggcgatgata tccgtagatg 3540 tacctggaca tccaggtgat gccggcggcg gtggtggagg cgcgcggaaa gtcgcggacg 3600 cggttccaga tgttgcgcag cggcaaaaag tgctccatgg tcgggacgct ctggccggtc 3660 aggcgcgcgc aatcgttgac gctctagcgt gcaaaaggag agcctgtaag cgggcactct 3720 tccgtggtct ggtggataaa ttcgcaaggg tatcatggcg gacgaccggg gttcgagccc 3780 cgtatccggc cgtccgccgt gatccatgcg gttaccgccc gcgtgtcgaa cccaggtgtg 3840 cgacgtcaga caacggggga gtgctccttt tggcttcctt ccaggcgcgg cggctgctgc 3900 gctagctttt ttggccactg gccgcgcgca gcgtaagcgg ttaggctgga aagcgaaagc 3960 attaagtggc tcgctccctg tagccggagg gttattttcc aagggttgag tcgcgggacc 4020 cccggttcga gtctcggacc ggccggactg cggcgaacgg gggtttgcct ccccgtcatg 4080 caagaccccg cttgcaaatt cctccggaaa cagggacgag cccctttttt gcttttcgtt 4140 taaac 4145 <210> 20 <211> 4247 <212> DNA <213> Artificial Sequence <220> <223> preparation example3 <400> 20 gtatacagac ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct cgtatagaaa 60 ctcggaccac tctgagacaa aggctcgcgt ccaggccagc acgaaggagg ctaagtggga 120 ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa gacacatgtc 180 gccctcttcg gcatcaagga aggtgattgg tttgtaggtg taggccacgt gaccgggtgt 240 tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct cttccgcatc 300 gctgtctgcg agggccagct gttggggtga gtactccctc tgaaaagcgg gcatgacttc 360 tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt 420 gatgcctttg agggtggccg catccatctg gtcagaaaag acaatctttt tgttgtcaag 480 cttggtggca aacgacccgt agagggcgtt ggacagcaac ttggcgatgg agcgcagggt 540 ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca cgtattcgcg 600 cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca ggtgcacgcg 660 ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc cgcgtaggcg 720 ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag aatggcggta gggggtctag 780 ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc ccgggcagca ggcgcgcgtc 840 gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc gggcggcaag 900 cgcgcgctcg tatgggttga gtgggggacc ccatggcatg gggtgggtga gcgcggaggc 960 gtacatgccg caaatgtcgt aaacgtagag gggctctctg agtattccaa gatatgtagg 1020 gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt gcgagggagc 1080 gaggaggtcg ggaccgaggt tgctacgggc gggctgctct gctcggaaga ctatctgcct 1140 gaagatggca tgtgagttgg atgatatggt tggacgctgg aagacgttga agctggcgtc 1200 tgtgagacct accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct tgttgaccag 1260 ctcggcggtg acctgcacgt ctagggcgca gtagtccagg gtttccttga tgatgtcata 1320 cttatcctgt cccttttttt tccacagctc gcggttgagg acaaactctt cgcggtcttt 1380 ccagtactct tggatcggaa acccgtcggc ctccgaacgg taagagccta gcatgtagaa 1440 ctggttgacg gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt atgcctgcgc 1500 ggccttccgg catgaccagc atgaagggca cgagctgctt cccaaaggcc cccatccaag 1560 tataggtctc tacatcgtag gtgacaaaga gacgctcggt gcgaggatgc gagccgatcg 1620 ggaagaactg gatctcccgc caccaattgg aggagtggct attgatgtgg tgaaagtaga 1680 agtccctgcg acgggccgaa cactcgtgct ggcttttgta aaaacgtgcg cagtactggc 1740 agcggtgcac gggctgtaca tcctgcacga ggttgacctg acgaccgcgc acaaggaagc 1800 agagtgggaa tttgagcccc tcgcctggcg ggtttggctg gtggtcttct acttcggctg 1860 cttgtccttg accgtctggc tgctcgaggg gagttacggt ggatcggacc accacgccgc 1920 gcgagcccaa agtccagatg tccgcgcgcg gcggtcggag cttgatgaca acatcgcgca 1980 gatgggagct gtccatggtc tggagctccc gcggcgtcag gtcaggcggg agctcctgca 2040 ggtttacctc gcatagacgg gtcagggcgc gggctagatc caggtgatac ctaatttcca 2100 ggggctggtt ggtggcggcg tcgatggctt gcaagaggcc gcatccccgc ggcgcgacta 2160 cggtaccgcg cggcgggcgg tgggccgcgg gggtgtcctt ggatgatgca tctaaaagcg 2220 gtgacgcggg cgagcccccg gaggtagggg gggctccgga cccgccggga gagggggcag 2280 gggcacgtcg gcgccgcgcg cgggcaggag ctggtgctgc gcgcgtaggt tgctggcgaa 2340 cgcgacgacg cggcggttga tctcctgaat ctggcgcctc tgcgtgaaga cgaggggccc 2400 ggtgagcttg aacctgaaag agagttcgac agaatcaatt tcggtgtcgt tgacggcggc 2460 ctggcgcaaa atctcctgca cgtctcctga gttgtcttga taggcgatct cggccatgaa 2520 ctgctcgatc tcttcctcct ggagatctcc gcgtccggct cgctccacgg tggcggcgag 2580 gtcgttggaa atgcgggcca tgagctgcga gaaggcgttg aggcctccct cgttccagac 2640 gcggctgtag accacgcccc cttcggcatc gcgggcgcgc atgaccacct gcgcgagatt 2700 gagctccacg tgccgggcga agacggcgta gtttcgcagg cgctgaaaga ggtagttgag 2760 ggtggtggcg gtgtgttctg ccacgaagaa gtacataacc cagcgtcgca acgtggattc 2820 gttgataatt gttgtgtagg tactccgccg ccgagggacc tgagcgagtc cgcatcgacc 2880 ggatcggaaa acctctcgag aaaggcgtct aaccagtcac agtcgcaagg taggctgagc 2940 accgtggcgg gcggcagcgg gcggcggtcg gggttgtttc tggcggaggt gctgctgatg 3000 atgtaattaa agtaggcggt cttgagacgg cggatggtcg acagaagcac catgtccttg 3060 ggtccggcct gctgaatgcg caggcggtcg gccatgcccc aggcttcgtt ttgacatcgg 3120 cgcaggtctt tgtagtagtc ttgcatgagc ctttctaccg gcacttcttc ttctccttcc 3180 tcttgtcctg catctcttgc atctatcgct gcggcggcgg cggagtttgg ccgtaggtgg 3240 cgccctcttc ctcccatgcg tgtgaccccg aagcccctca tcggctgaag cagggctagg 3300 tcggcgacaa cgcgctcggc taatatggcc tgctgcacct gcgtgagggt agactggaag 3360 tcatccatgt ccacaaagcg gtggtatgcg cccgtgttga tggtgtaagt gcagttggcc 3420 ataacggacc agttaacggt ctggtgaccc ggctgcgaga gctcggtgta cctgagacgc 3480 gagtaagccc tcgagtcaaa tacgtagtcg ttgcaagtcc gcaccaggta ctggtatccc 3540 accaaaaagt gcggcggcgg ctggcggtag aggggccagc gtagggtggc cggggctccg 3600 ggggcgagat cttccaacat aaggcgatga tatccgtaga tgtacctgga catccaggtg 3660 atgccggcgg cggtggtgga ggcgcgcgga aagtcgcgga cgcggttcca gatgttgcgc 3720 agcggcaaaa agtgctccat ggtcgggacg ctctggccgg tcaggcgcgc gcaatcgttg 3780 acgctctagc gtgcaaaagg agagcctgta agcgggcact cttccgtggt ctggtggata 3840 aattcgcaag ggtatcatgg cggacgaccg gggttcgagc cccgtatccg gccgtccgcc 3900 gtgatccatg cggttaccgc ccgcgtgtcg aacccaggtg tgcgacgtca gacaacgggg 3960 gagtgctcct tttggcttcc ttccaggcgc ggcggctgct gcgctagctt ttttggccac 4020 tggccgcgcg cagcgtaagc ggttaggctg gaaagcgaaa gcattaagtg gctcgctccc 4080 tgtagccgga gggttatttt ccaagggttg agtcgcggga cccccggttc gagtctcgga 4140 ccggccggac tgcggcgaac gggggtttgc ctccccgtca tgcaagaccc cgcttgcaaa 4200 ttcctccgga aacagggacg agcccctttt ttgcttttcg tttaaac 4247 <210> 21 <211> 3632 <212> DNA <213> Artificial Sequence <220> <223> preparation example4 <400> 21 gtatacagac ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct cgtatagaaa 60 ctcggaccac tctgagacaa aggctcgcgt ccaggccagc acgaaggagg ctaagtggga 120 ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa gacacatgtc 180 gccctcttcg gcatcaagga aggtgattgg tttgtaggtg taggccacgt gaccgggtgt 240 tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct cttccgcatc 300 gctgtctgcg agggccagct gttggggtga gtactccctc tgaaaagcgg gcatgacttc 360 tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt 420 gatgcctttg agggtggccg catccatctg gtcagaaaag acaatctttt tgttgtcaag 480 cttggtggca aacgacccgt agagggcgtt ggacagcaac ttggcgatgg agcgcagggt 540 ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca cgtattcgcg 600 cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca ggtgcacgcg 660 ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc cgcgtaggcg 720 ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag aatggcggta gggggtctag 780 ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc ccgggcagca ggcgcgcgtc 840 gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc gggcggcaag 900 cgcgcgctcg tatgggttga gtgggggacc ccatggcatg gggtgggtga gcgcggaggc 960 gtacatgccg caaatgtcgt aaacgtagag gggctctctg agtattccaa gatatgtagg 1020 gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt gcgagggagc 1080 gaggaggtcg ggaccgaggt tgctacgggc gggctgctct gctcggaaga ctatctgcct 1140 gaagatggca tgtgagttgg atgatatggt tggacgctgg aagacgttga agctggcgtc 1200 tgtgagacct accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct tgttgaccag 1260 ctcggcggtg acctgcacgt ctagggcgca gtagtccagg gtttccttga tgatgtcata 1320 cttatcctgt cccttttttt tccacagctc gcggttgagg acaaactctt cgcggtcttt 1380 ccagtactct tggatcggaa acccgtcggc ctccgaacgg taagagccta gcatgtagaa 1440 ctggttgacg gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt atgcctgcgc 1500 ggccttccgg catgaccagc atgaagggca cgagctgctt cccaaaggcc cccatccaag 1560 tataggtctc tacatcgtag gtgacaaaga gacgctcggt gcgaggatgc gagccgatcg 1620 ggaagaactg gatctcccgc caccaattgg aggagtggct attgatgtgg tgaaagtaga 1680 agtccctgcg acgggccgaa cactcgtgct ggcttttgta aaaacgtgcg cagtactggc 1740 agcggtgcac gggctgtaca tcctgcacga ggttgacctg acgaccgcgc acaaggaagc 1800 agagtgggaa tttgagcccc tcgcctggcg ggtttggctg gtggtcttct acttcggctg 1860 cttgtccttg accgtctggc tgctcgaggg gagttacggt ggatcggacc accacgccgc 1920 gcgagcccaa agtccagatg tccgcgcgcg gcggtcggag cttgatgaca acatcgcgca 1980 gatgggagct gtccatggtc tggagctccc gcggcgtcag gtcaggcggg agctcctgca 2040 ggtttacctc gcatagacgg gtcagggcgc gggctagatc caggtgatac ctaatttcca 2100 ggggctggtt ggtggcggcg tcgatggctt gcaagaggcc gcatccccgc ggcgcgacta 2160 cggtaccgcg cggcgggcgg tgggccgcgg gggtgtcctt ggatgatgca taattgttgt 2220 gtaggtactc cgccgccgag ggacctgagc gagtccgcat cgaccggatc ggaaaacctc 2280 tcgagaaagg cgtctaacca gtcacagtcg caaggtaggc tgagcaccgt ggcgggcggc 2340 agcgggcggc ggtcggggtt gtttctggcg gaggtgctgc tgatgatgta attaaagtag 2400 gcggtcttga gacggcggat ggtcgacaga agcaccatgt ccttgggtcc ggcctgctga 2460 atgcgcaggc ggtcggccat gccccaggct tcgttttgac atcggcgcag gtctttgtag 2520 tagtcttgca tgagcctttc taccggcact tcttcttctc cttcctcttg tcctgcatct 2580 cttgcatcta tcgctgcggc ggcggcggag tttggccgta ggtggcgccc tcttcctccc 2640 atgcgtgtga ccccgaagcc cctcatcggc tgaagcaggg ctaggtcggc gacaacgcgc 2700 tcggctaata tggcctgctg cacctgcgtg agggtagact ggaagtcatc catgtccaca 2760 aagcggtggt atgcgcccgt gttgatggtg taagtgcagt tggccataac ggaccagtta 2820 acggtctggt gacccggctg cgagagctcg gtgtacctga gacgcgagta agccctcgag 2880 tcaaatacgt agtcgttgca agtccgcacc aggtactggt atcccaccaa aaagtgcggc 2940 ggcggctggc ggtagagggg ccagcgtagg gtggccgggg ctccgggggc gagatcttcc 3000 aacataaggc gatgatatcc gtagatgtac ctggacatcc aggtgatgcc ggcggcggtg 3060 gtggaggcgc gcggaaagtc gcggacgcgg ttccagatgt tgcgcagcgg caaaaagtgc 3120 tccatggtcg ggacgctctg gccggtcagg cgcgcgcaat cgttgacgct ctagcgtgca 3180 aaaggagagc ctgtaagcgg gcactcttcc gtggtctggt ggataaattc gcaagggtat 3240 catggcggac gaccggggtt cgagccccgt atccggccgt ccgccgtgat ccatgcggtt 3300 accgcccgcg tgtcgaaccc aggtgtgcga cgtcagacaa cgggggagtg ctccttttgg 3360 cttccttcca ggcgcggcgg ctgctgcgct agcttttttg gccactggcc gcgcgcagcg 3420 taagcggtta ggctggaaag cgaaagcatt aagtggctcg ctccctgtag ccggagggtt 3480 attttccaag ggttgagtcg cgggaccccc ggttcgagtc tcggaccggc cggactgcgg 3540 cgaacggggg tttgcctccc cgtcatgcaa gaccccgctt gcaaattcct ccggaaacag 3600 ggacgagccc cttttttgct tttcgtttaa ac 3632 <210> 22 <211> 2930 <212> DNA <213> Artificial Sequence <220> <223> preparation example5 <400> 22 gtatacagac ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct cgtatagaaa 60 ctcggaccac tctgagacaa aggctcgcgt ccaggccagc acgaaggagg ctaagtggga 120 ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa gacacatgtc 180 gccctcttcg gcatcaagga aggtgattgg tttgtaggtg taggccacgt gaccgggtgt 240 tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct cttccgcatc 300 gctgtctgcg agggccagct gttggggtga gtactccctc tgaaaagcgg gcatgacttc 360 tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt 420 gatgcctttg agggtggccg catccatctg gtcagaaaag acaatctttt tgttgtcaag 480 cttggtggca aacgacccgt agagggcgtt ggacagcaac ttggcgatgg agcgcagggt 540 ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca cgtattcgcg 600 cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca ggtgcacgcg 660 ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc cgcgtaggcg 720 ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag aatggcggta gggggtctag 780 ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc ccgggcagca ggcgcgcgtc 840 gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc gggcggcaag 900 cgcgcgctcg tatgggttga gtgggggacc ccatggcatg gggtgggtga gcgcggaggc 960 gtacatgccg caaatgtcgt aaacgtagag gggctctctg agtattccaa gatatgtagg 1020 gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt gcgagggagc 1080 gaggaggtcg ggaccgaggt tgctacgggc gggctgctct gctcggaaga ctatctgcct 1140 gaagatggca tgtgagttgg atgatatggt tggacgctgg aagacgttga agctggcgtc 1200 tgtgagacct accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct tgttgaccag 1260 ctcggcggtg acctgcacgt ctagggcgca gtagtccagg gtttccttga tgatgtcata 1320 cttatcctgt cccttttttt tccacagctc gcggttgagg acaaactctt cgcggtcttt 1380 ccagtactct tggatcggaa acccgtcggc ctccgaacgg taagagccta gcatgtagaa 1440 ctggttgacg gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt atgcctgcgc 1500 ggccttccgg attgttgtgt aggtactccg ccgccgaggg acctgagcga gtccgcatcg 1560 accggatcgg aaaacctctc gagaaaggcg tctaaccagt cacagtcgca aggtaggctg 1620 agcaccgtgg cgggcggcag cgggcggcgg tcggggttgt ttctggcgga ggtgctgctg 1680 atgatgtaat taaagtaggc ggtcttgaga cggcggatgg tcgacagaag caccatgtcc 1740 ttgggtccgg cctgctgaat gcgcaggcgg tcggccatgc cccaggcttc gttttgacat 1800 cggcgcaggt ctttgtagta gtcttgcatg agcctttcta ccggcacttc ttcttctcct 1860 tcctcttgtc ctgcatctct tgcatctatc gctgcggcgg cggcggagtt tggccgtagg 1920 tggcgccctc ttcctcccat gcgtgtgacc ccgaagcccc tcatcggctg aagcagggct 1980 aggtcggcga caacgcgctc ggctaatatg gcctgctgca cctgcgtgag ggtagactgg 2040 aagtcatcca tgtccacaaa gcggtggtat gcgcccgtgt tgatggtgta agtgcagttg 2100 gccataacgg accagttaac ggtctggtga cccggctgcg agagctcggt gtacctgaga 2160 cgcgagtaag ccctcgagtc aaatacgtag tcgttgcaag tccgcaccag gtactggtat 2220 cccaccaaaa agtgcggcgg cggctggcgg tagaggggcc agcgtagggt ggccggggct 2280 ccgggggcga gatcttccaa cataaggcga tgatatccgt agatgtacct ggacatccag 2340 gtgatgccgg cggcggtggt ggaggcgcgc ggaaagtcgc ggacgcggtt ccagatgttg 2400 cgcagcggca aaaagtgctc catggtcggg acgctctggc cggtcaggcg cgcgcaatcg 2460 ttgacgctct agcgtgcaaa aggagagcct gtaagcgggc actcttccgt ggtctggtgg 2520 ataaattcgc aagggtatca tggcggacga ccggggttcg agccccgtat ccggccgtcc 2580 gccgtgatcc atgcggttac cgcccgcgtg tcgaacccag gtgtgcgacg tcagacaacg 2640 ggggagtgct ccttttggct tccttccagg cgcggcggct gctgcgctag cttttttggc 2700 cactggccgc gcgcagcgta agcggttagg ctggaaagcg aaagcattaa gtggctcgct 2760 ccctgtagcc ggagggttat tttccaaggg ttgagtcgcg ggacccccgg ttcgagtctc 2820 ggaccggccg gactgcggcg aacgggggtt tgcctccccg tcatgcaaga ccccgcttgc 2880 aaattcctcc ggaaacaggg acgagcccct tttttgcttt tcgtttaaac 2930 <210> 23 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 23 cgatcgggta ccgccaccat ggagctggcg gccttgtg 38 <210> 24 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 24 cgatcgaagc ttttactaga tcccaaagac cacccccaag a 41 <210> 25 <211> 6 <212> DNA <213> Artificial Sequence <220> <223> Kozak sequence <400> 25 gccacc 6 <210> 26 <211> 6 <212> DNA <213> Artificial Sequence <220> <223> stop codon <400> 26 tacta 6 <210> 27 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> Noncoding DNA <400> 27 atggccttga gcgtcaacga ttgcgcgcgc ctgaccggcc agagcgtccc gacc 54 <210> 28 <211> 9 <212> DNA <213> Artificial Sequence <220> <223> Kozak sequence <400> 28 gccgccacc 9 <210> 29 <211> 426 <212> DNA <213> Artificial Sequence <220> <223> Noncoding DNA <400> 29 atggccttgg ctcaagctca ccgggcccgt cgtcttcacg cagaggcgcc agattcagga 60 gatcaaccgc cgcgtcgtcg cgttcgccag caacctacgc gcgcagcacc agctcctgcc 120 cgcgcgcggc gccgacgtgc ccctgccccc tctcccggcg ggtccggagc cccccctacc 180 tccgggggct cgcccgcgtc accgctttta gatgcatcat ccaaggacac ccccgcggcc 240 caccgcccgc cgcgcggtac cgtagtcgcg ccgcggggat gcggcctctt gcaagccatc 300 gacgccgcca ccaaccagcc cctggaaatt aggtatcacc tggatctagc ccgcgccctg 360 acccgtctat gcgaggtaaa cctgcaggag ctcccgcctg acctgacgcc gcgggagctc 420 cagacc 426 <210> 30 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 30 gctcggaaga ctatctgcct 20 <210> 31 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 31 tactcgcgtc tcaggtacac 20 <210> 32 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 32 gtaaaacgac ggccagt 17 <210> 33 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 33 gcggataaca atttcacaca gg 22 <210> 34 <211> 35938 <212> DNA <213> Homo sapiens <400> 34 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagatgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccat gagcaccaac tcgtttgatg gaagcattgt gagctcatat ttgacaacgc 3660 gcatgccccc atgggccggg gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc 3720 ccgtcctgcc cgcaaactct actaccttga cctacgagac cgtgtctgga acgccgttgg 3780 agactgcagc ctccgccgcc gcttcagccg ctgcagccac cgcccgcggg attgtgactg 3840 actttgcttt cctgagcccg cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg 3900 acaagttgac ggctcttttg gcacaattgg attctttgac ccgggaactt aatgtcgttt 3960 ctcagcagct gttggatctg cgccagcagg tttctgccct gaaggcttcc tcccctccca 4020 atgcggttta aaacataaat aaaaaaccag actctgtttg gatttggatc aagcaagtgt 4080 cttgctgtct ttatttaggg gttttgcgcg cgcggtaggc ccgggaccag cggtctcggt 4140 cgttgagggt cctgtgtatt ttttccagga cgtggtaaag gtgactctgg atgttcagat 4200 acatgggcat aagcccgtct ctggggtgga ggtagcacca ctgcagagct tcatgctgcg 4260 gggtggtgtt gtagatgatc cagtcgtagc aggagcgctg ggcgtggtgc ctaaaaatgt 4320 ctttcagtag caagctgatt gccaggggca ggcccttggt gtaagtgttt acaaagcggt 4380 taagctggga tgggtgcata cgtggggata tgagatgcat cttggactgt atttttaggt 4440 tggctatgtt cccagccata tccctccggg gattcatgtt gtgcagaacc accagcacag 4500 tgtatccggt gcacttggga aatttgtcat gtagcttaga aggaaatgcg tggaagaact 4560 tggagacgcc cttgtgacct ccaagatttt ccatgcattc gtccataatg atggcaatgg 4620 gcccacgggc ggcggcctgg gcgaagatat ttctgggatc actaacgtca tagttgtgtt 4680 ccaggatgag atcgtcatag gccattttta caaagcgcgg gcggagggtg ccagactgcg 4740 gtataatggt tccatccggc ccaggggcgt agttaccctc acagatttgc atttccccacg 4800 ctttgagttc agatgggggg atcatgtcta cctgcggggc gatgaagaaa acggtttccg 4860 gggtagggga gatcagctgg gaagaaagca ggttcctgag cagctgcgac ttaccgcagc 4920 cggtgggccc gtaaatcaca cctattaccg ggtgcaactg gtagttaaga gagctgcagc 4980 tgccgtcatc cctgagcagg ggggccactt cgttaagcat gtccctgact cgcatgtttt 5040 ccctgaccaa atccgccaga aggcgctcgc cgcccagcga tagcagttct tgcaaggaag 5100 caaagttttt caacggtttg agaccgtccg ccgtaggcat gcttttgagc gtttgaccaa 5160 gcagttccag gcggtcccac agctcggtca cctgctctac ggcatctcga tccagcatat 5220 ctcctcgttt cgcgggttgg ggcggctttc gctgtacggc agtagtcggt gctcgtccag 5280 acgggccagg gtcatgtctt tccacgggcg cagggtcctc gtcagcgtag tctgggtcac 5340 ggtgaagggg tgcgctccgg gctgcgcgct ggccagggtg cgcttgaggc tggtcctgct 5400 ggtgctgaag cgctgccggt cttcgccctg cgcgtcggcc aggtagcatt tgaccatggt 5460 gtcatagtcc agcccctccg cggcgtggcc cttggcgcgc agcttgccct tggaggaggc 5520 gccgcacgag gggcagtgca gacttttgag ggcgtagagc ttgggcgcga gaaataccga 5580 ttccggggag taggcatccg cgccgcaggc cccgcagacg gtctcgcatt ccacgagcca 5640 ggtgagctct ggccgttcgg ggtcaaaaac caggtttccc ccatgctttt tgatgcgttt 5700 cttacctctg gtttccatga gccggtgtcc acgctcggtg acgaaaaggc tgtccgtgtc 5760 cccgtataca gacttgagag gcctgtcctc gagcggtgtt ccgcggtcct cctcgtatag 5820 aaactcggac cactctgaga caaaggctcg cgtccaggcc agcacgaagg aggctaagtg 5880 ggaggggtag cggtcgttgt ccactagggg gtccactcgc tccagggtgt gaagacacat 5940 gtcgccctct tcggcatcaa ggaaggtgat tggtttgtag gtgtaggcca cgtgaccggg 6000 tgttcctgaa ggggggctat aaaagggggt gggggcgcgt tcgtcctcac tctcttccgc 6060 atcgctgtct gcgagggcca gctgttgggg tgagtactcc ctctgaaaag cgggcatgac 6120 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccgc 6180 ggtgatgcct ttgagggtgg ccgcatccat ctggtcagaa aagacaatct ttttgttgtc 6240 aagcttggtg gcaaacgacc cgtagagggc gttggacagc aacttggcga tggagcgcag 6300 ggtttggttt ttgtcgcgat cggcgcgctc cttggccgcg atgtttagct gcacgtattc 6360 gcgcgcaacg caccgccatt cgggaaagac ggtggtgcgc tcgtcgggca ccaggtgcac 6420 gcgccaaccg cggttgtgca gggtgacaag gtcaacgctg gtggctacct ctccgcgtag 6480 gcgctcgttg gtccagcaga ggcggccgcc cttgcgcgag cagaatggcg gtagggggtc 6540 tagctgcgtc tcgtccgggg ggtctgcgtc cacggtaaag accccgggca gcaggcgcgc 6600 gtcgaagtag tctatcttgc atccttgcaa gtctagcgcc tgctgccatg cgcgggcggc 6660 aagcgcgcgc tcgtatgggt tgagtggggg accccatggc atggggtggg tgagcgcgga 6720 ggcgtacat ccgcaaatgt cgtaaacgta gaggggctct ctgagtattc caagatatgt 6780 agggtagcat cttccaccgc ggatgctggc gcgcacgtaa tcgtatagtt cgtgcgaggg 6840 agcgaggagg tcgggaccga ggttgctacg ggcgggctgc tctgctcgga agactatctg 6900 cctgaagatg gcatgtgagt tggatgatat ggttggacgc tggaagacgt tgaagctggc 6960 gtctgtgaga cctaccgcgt cacgcacgaa ggaggcgtag gagtcgcgca gcttgttgac 7020 cagctcggcg gtgacctgca cgtctagggc gcagtagtcc agggtttcct tgatgatgtc 7080 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 7140 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtaagagc ctagcatgta 7200 gaactggttg acggcctggt aggcgcagca tcccttttct acgggtagcg cgtatgcctg 7260 cgcggccttc cggagcgagg tgtgggtgag cgcaaaggtg tccctgacca tgactttgag 7320 gtactggtat ttgaagtcag tgtcgtcgca tccgccctgc tcccagagca aaaagtccgt 7380 gcgctttttg gaacgcggat ttggcagggc gaaggtgaca tcgttgaaga gtatctttcc 7440 cgcgcgaggc ataaagttgc gtgtgatgcg gaagggtccc ggcacctcgg aacggttgtt 7500 aattacctgg gcggcgagca cgatctcgtc aaagccgttg atgttgtggc ccacaatgta 7560 aagttccaag aagcgcggga tgcccttgat ggaaggcaat tttttaagtt cctcgtaggt 7620 gagctcttca ggggagctga gcccgtgctc tgaaagggcc cagtctgcaa gatgagggtt 7680 ggaagcgacg aatgagctcc acaggtcacg ggccattagc atttgcaggt ggtcgcgaaa 7740 ggtcctaaac tggcgaccta tggccatttt ttctggggtg atgcagtaga aggtaagcgg 7800 gtcttgttcc cagcggtccc atccaaggtt cgcggctagg tctcgcgcgg cagtcactag 7860 aggctcatct ccgccgaact tcatgaccag catgaagggc acgagctgct tcccaaaggc 7920 ccccatccaa gtataggtct ctacatcgta ggtgacaaag agacgctcgg tgcgaggatg 7980 cgagccgatc gggaagaact ggatctcccg ccaccaattg gaggagtggc tattgatgtg 8040 gtgaaagtag aagtccctgc gacgggccga acactcgtgc tggcttttgt aaaaacgtgc 8100 gcagtactgg cagcggtgca cgggctgtac atcctgcacg aggttgacct gacgaccgcg 8160 cacaaggaag cagagtggga atttgagccc ctcgcctggc gggtttggct ggtggtcttc 8220 tacttcggct gcttgtcctt gaccgtctgg ctgctcgagg ggagttacgg tggatcggac 8280 caccacgccg cgcgagccca aagtccagat gtccgcgcgc ggcggtcgga gcttgatgac 8340 aacatcgcgc agatgggagc tgtccatggt ctggagctcc cgcggcgtca ggtcaggcgg 8400 gagctcctgc aggtttacct cgcatagacg ggtcagggcg cgggctagat ccaggtgata 8460 cctaatttcc aggggctggt tggtggcggc gtcgatggct tgcaagaggc cgcatccccg 8520 cggcgcgact acggtaccgc gcggcgggcg gtgggccgcg ggggtgtcct tggatgatgc 8580 atctaaaagc ggtgacgcgg gcgagccccc ggaggtaggg ggggctccgg acccgccggg 8640 agagggggca ggggcacgtc ggcgccgcgc gcgggcagga gctggtgctg cgcgcgtagg 8700 ttgctggcga acgcgacgac gcggcggttg atctcctgaa tctggcgcct ctgcgtgaag 8760 acgacgggcc cggtgagctt gagcctgaaa gagagttcga cagaatcaat ttcggtgtcg 8820 ttgacggcgg cctggcgcaa aatctcctgc acgtctcctg agttgtcttg ataggcgatc 8880 tcggccatga actgctcgat ctcttcctcc tggagatctc cgcgtccggc tcgctccacg 8940 gtggcggcga ggtcgttgga aatgcgggcc atgagctgcg agaaggcgtt gaggcctccc 9000 tcgttccaga cgcggctgta gaccacgccc ccttcggcat cgcgggcgcg catgaccacc 9060 tgcgcgagat tgagctccac gtgccgggcg aagacggcgt agtttcgcag gcgctgaaag 9120 aggtagttga gggtggtggc ggtgtgttct gccacgaaga agtacataac ccagcgtcgc 9180 aacgtggatt cgttgatatc ccccaaggcc tcaaggcgct ccatggcctc gtagaagtcc 9240 acggcgaagt tgaaaaactg ggagttgcgc gccgacacgg ttaactcctc ctccagaaga 9300 cggatgagct cggcgacagt gtcgcgcacc tcgcgctcaa aggctacagg ggcctcttct 9360 tcttcttcaa tctcctcttc cataagggcc tccccttctt cttcttctgg cggcggtggg 9420 ggagggggga cacggcggcg acgacggcgc accgggaggc ggtcgacaaa gcgctcgatc 9480 atctccccgc ggcgacggcg catggtctcg gtgacggcgc ggccgttctc gcgggggcgc 9540 agttggaaga cgccgcccgt catgtcccgg ttatgggttg gcggggggct gccatgcggc 9600 agggatacgg cgctaacgat gcatctcaac aattgttgtg taggtactcc gccgccgagg 9660 gacctgagcg agtccgcatc gaccggatcg gaaaacctct cgagaaaggc gtctaaccag 9720 tcacagtcgc aaggtaggct gagcaccgtg gcgggcggca gcgggcggcg gtcggggttg 9780 tttctggcgg aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag acggcggatg 9840 gtcgacagaa gcaccatgtc cttgggtccg gcctgctgaa tgcgcaggcg gtcggccatg 9900 ccccaggctt cgttttgaca tcggcgcagg tctttgtagt agtcttgcat gagcctttct 9960 accggcactt cttcttctcc ttcctcttgt cctgcatctc ttgcatctat cgctgcggcg 10020 gcggcggagt ttggccgtag gtggcgccct cttcctccca tgcgtgtgac cccgaagccc 10080 ctcatcggct gaagcagggc taggtcggcg acaacgcgct cggctaatat ggcctgctgc 10140 acctgcgtga gggtagactg gaagtcatcc atgtccacaa agcggtggta tgcgcccgtg 10200 ttgatggtgt aagtgcagtt ggccataacg gaccagttaa cggtctggtg acccggctgc 10260 gagagctcgg tgtacctgag acgcgagtaa gccctcgagt caaatacgta gtcgttgcaa 10320 gtccgcacca ggtactggta tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc 10380 cagcgtaggg tggccggggc tccgggggcg agatcttcca acataaggcg atgatatccg 10440 tagatgtacc tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg 10500 cggacgcggt tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg 10560 ccggtcaggc gcgcgcaatc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg 10620 ggcactcttc cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt 10680 tcgagccccg tatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc 10740 caggtgtgcg acgtcagaca acgggggagt gctccttttg gcttccttcc aggcgcggcg 10800 gctgctgcgc tagctttttt ggccactggc cgcgcgcagc gtaagcggtt aggctggaaa 10860 gcgaaagcat taagtggctc gctccctgta gccggagggt tattttccaa gggttgagtc 10920 gcgggacccc cggttcgagt ctcggaccgg ccggactgcg gcgaacgggg gtttgcctcc 10980 ccgtcatgca agaccccgct tgcaaattcc tccggaaaca gggacgagcc ccttttttgc 11040 ttttcccaga tgcatccggt gctgcggcag atgcgccccc ctcctcagca gcggcaagag 11100 caagagcagc ggcagacatg cagggcaccc tcccctcctc ctaccgcgtc aggaggggcg 11160 acatccgcgg ttgacgcggc agcagatggt gattacgaac ccccgcggcg ccgggcccgg 11220 cactacctgg acttggagga gggcgagggc ctggcgcggc taggagcgcc ctctcctgag 11280 cggtacccaa gggtgcagct gaagcgtgat acgcgtgagg cgtacgtgcc gcggcagaac 11340 ctgtttcgcg accgcgaggg agaggagccc gaggagatgc gggatcgaaa gttccacgca 11400 gggcgcgagc tgcggcatgg cctgaatcgc gagcggttgc tgcgcgagga ggactttgag 11460 cccgacgcgc gaaccgggat tagtcccgcg cgcgcacacg tggcggccgc cgacctggta 11520 accgcatacg agcagacggt gaaccaggag attaactttc aaaaaagctt taacaaccac 11580 gtgcgtacgc ttgtggcgcg cgaggaggtg gctataggac tgatgcatct gtgggacttt 11640 gtaagcgcgc tggagcaaaa cccaaatagc aagccgctca tggcgcagct gttccttata 11700 gtgcagcaca gcagggacaa cgaggcattc agggatgcgc tgctaaacat agtagagccc 11760 gagggccgct ggctgctcga tttgataaac atcctgcaga gcatagtggt gcaggagcgc 11820 agcttgagcc tggctgacaa ggtggccgcc atcaactatt ccatgcttag cctgggcaag 11880 ttttacgccc gcaagatata ccatacccct tacgttccca tagacaagga ggtaaagatc 11940 gaggggttct acatgcgcat ggcgctgaag gtgcttacct tgagcgacga cctgggcgtt 12000 tatcgcaacg agcgcatcca caaggccgtg agcgtgagcc ggcggcgcga gctcagcgac 12060 cgcgagctga tgcacagcct gcaaagggcc ctggctggca cgggcagcgg cgatagagag 12120 gccgagtcct actttgacgc gggcgctgac ctgcgctggg ccccaagccg acgcgccctg 12180 gaggcagctg gggccggacc tgggctggcg gtggcacccg cgcgcgctgg caacgtcggc 12240 ggcgtggagg aatatgacga ggacgatgag tacgagccag aggacggcga gtactaagcg 12300 gtgatgtttc tgatcagatg atgcaagacg caacggaccc ggcggtgcgg gcggcgctgc 12360 agagccagcc gtccggcctt aactccacgg acgactggcg ccaggtcatg gaccgcatca 12420 tgtcgctgac tgcgcgcaat cctgacgcgt tccggcagca gccgcaggcc aaccggctct 12480 ccgcaattct ggaagcggtg gtcccggcgc gcgcaaaccc cacgcacgag aaggtgctgg 12540 cgatcgtaaa cgcgctggcc gaaaacaggg ccatccggcc cgacgaggcc ggcctggtct 12600 acgacgcgct gcttcagcgc gtggctcgtt acaacagcgg caacgtgcag accaacctgg 12660 accggctggt gggggatgtg cgcgaggccg tggcgcagcg tgagcgcgcg cagcagcagg 12720 gcaacctggg ctccatggtt gcactaaacg ccttcctgag tacacagccc gccaacgtgc 12780 cgcggggaca ggaggactac accaactttg tgagcgcact gcggctaatg gtgactgaga 12840 caccgcaaag tgaggtgtac cagtctgggc cagactattt tttccagacc agtagacaag 12900 gcctgcagac cgtaaacctg agccaggctt tcaaaaactt gcaggggctg tggggggtgc 12960 gggctcccac aggcgaccgc gcgaccgtgt ctagcttgct gacgcccaac tcgcgcctgt 13020 tgctgctgct aatagcgccc ttcacggaca gtggcagcgt gtcccgggac acatacctag 13080 gtcacttgct gacactgtac cgcgaggcca taggtcaggc gcatgtggac gagcatactt 13140 tccaggagat tacaagtgtc agccgcgcgc tggggcagga ggacacgggc agcctggagg 13200 caaccctaaa ctacctgctg accaaccggc ggcagaagat cccctcgttg cacagtttaa 13260 acagcgagga ggagcgcatt ttgcgctacg tgcagcagag cgtgagcctt aacctgatgc 13320 gcgacggggt aacgcccagc gtggcgctgg acatgaccgc gcgcaacatg gaaccgggca 13380 tgtatgcctc aaaccggccg tttatcaacc gcctaatgga ctacttgcat cgcgcggccg 13440 ccgtgaaccc cgagtatttc accaatgcca tcttgaaccc gcactggcta ccgccccctg 13500 gtttctacac cgggggattc gaggtgcccg agggtaacga tggattcctc tgggacgaca 13560 tagacgacag cgtgttttcc ccgcaaccgc agaccctgct agagttgcaa cagcgcgagc 13620 aggcagaggc ggcgctgcga aaggaaagct tccgcaggcc aagcagcttg tccgatctag 13680 gcgctgcggc cccgcggtca gatgctagta gcccatttcc aagcttgata gggtctctta 13740 ccagcactcg caccacccgc ccgcgcctgc tgggcgagga ggagtaccta aacaactcgc 13800 tgctgcagcc gcagcgcgaa aaaaacctgc ctccggcatt tcccaacaac gggatagaga 13860 gcctagtgga caagatgagt agatggaaga cgtacgcgca ggagcacagg gacgtgccag 13920 gcccgcgccc gcccacccgt cgtcaaaggc acgaccgtca gcggggtctg gtgtgggagg 13980 acgatgactc ggcagacgac agcagcgtcc tggatttggg agggagtggc aacccgtttg 14040 cgcaccttcg ccccaggctg gggagaatgt tttaaaaaaa aaaaagcatg atgcaaaata 14100 aaaaactcac caaggccatg gcaccgagcg ttggttttct tgtattcccc ttagtatgcg 14160 gcgcgcggcg atgtatgagg aaggtcctcc tccctcctac gagagtgtgg tgagcgcggc 14220 gccagtggcg gcggcgctgg gttctccctt cgatgctccc ctggacccgc cgtttgtgcc 14280 tccgcggtac ctgcggccta ccggggggag aaacagcatc cgttactctg agttggcacc 14340 cctattcgac accacccgtg tgtacctggt ggacaacaag tcaacggatg tggcatccct 14400 gaactaccag aacgaccaca gcaactttct gaccacggtc attcaaaaca atgactacag 14460 cccgggggag gcaagcacac agaccatcaa tcttgacgac cggtcgcact ggggcggcga 14520 cctgaaaacc atcctgcata ccaacatgcc aaatgtgaac gagttcatgt ttaccaataa 14580 gtttaaggcg cgggtgatgg tgtcgcgctt gcctactaag gacaatcagg tggagctgaa 14640 atacgagtgg gtggagttca cgctgcccga gggcaactac tccgagacca tgaccataga 14700 ccttatgaac aacgcgatcg tggagcacta cttgaaagtg ggcagacaga acggggttct 14760 ggaaagcgac atcggggtaa agtttgacac ccgcaacttc agactggggt ttgaccccgt 14820 cactggtctt gtcatgcctg gggtatatac aaacgaagcc ttccatccag acatcatttt 14880 gctgccagga tgcggggtgg acttcaccca cagccgcctg agcaacttgt tgggcatccg 14940 caagcggcaa cccttccagg agggctttag gatcacctac gatgatctgg agggtggtaa 15000 cattcccgca ctgttggatg tggacgccta ccaggcgagc ttgaaagatg acaccgaaca 15060 gggcgggggt ggcgcaggcg gcagcaacag cagtggcagc ggcgcggaag agaactccaa 15120 cgcggcagcc gcggcaatgc agccggtgga ggacatgaac gatcatgcca ttcgcggcga 15180 cacctttgcc acacgggctg aggagaagcg cgctgaggcc gaagcagcgg ccgaagctgc 15240 cgccccccgct gcgcaacccg aggtcgagaa gcctcagaag aaaccggtga tcaaacccct 15300 gacagaggac agcaagaaac gcagttacaa cctaataagc aatgacagca ccttcaccca 15360 gtaccgcagc tggtaccttg catacaacta cggcgaccct cagaccggaa tccgctcatg 15420 gaccctgctt tgcactcctg acgtaacctg cggctcggag caggtctact ggtcgttgcc 15480 agacatgatg caagaccccg tgaccttccg ctccacgcgc cagatcagca actttccggt 15540 ggtgggcgcc gagctgttgc ccgtgcactc caagagcttc tacaacgacc aggccgtcta 15600 ctcccaactc atccgccagt ttacctctct gacccacgtg ttcaatcgct ttcccgagaa 15660 ccagattttg gcgcgcccgc cagcccccac catcaccacc gtcagtgaaa acgttcctgc 15720 tctcacagat cacgggacgc taccgctgcg caacagcatc ggaggagtcc agcgagtgac 15780 cattactgac gccagacgcc gcacctgccc ctacgtttac aaggccctgg gcatagtctc 15840 gccgcgcgtc ctatcgagcc gcactttttg agcaagcatg tccatcctta tatcgcccag 15900 caataacaca ggctggggcc tgcgcttccc aagcaagatg tttggcgggg ccaagaagcg 15960 ctccgaccaa cacccagtgc gcgtgcgcgg gcactaccgc gcgccctggg gcgcgcacaa 16020 acgcggccgc actgggcgca ccaccgtcga tgacgccatc gacgcggtgg tggaggaggc 16080 gcgcaactac acgcccacgc cgccaccagt gtccacagtg gacgcggcca ttcagaccgt 16140 ggtgcgcgga gcccggcgct atgctaaaat gaagagacgg cggaggcgcg tagcacgtcg 16200 ccaccgccgc cgacccggca ctgccgccca acgcgcggcg gcggccctgc ttaaccgcgc 16260 acgtcgcacc ggccgacggg cggccatgcg ggccgctcga aggctggccg cgggtattgt 16320 cactgtgccc cccaggtcca ggcgacgagc ggccgccgca gcagccgcgg ccattagtgc 16380 tatgactcag ggtcgcaggg gcaacgtgta ttgggtgcgc gactcggtta gcggcctgcg 16440 cgtgcccgtg cgcacccgcc ccccgcgcaa ctagattgca agaaaaaact acttagactc 16500 gtactgttgt atgtatccag cggcggcggc gcgcaacgaa gctatgtcca agcgcaaaat 16560 caaagaagag atgctccagg tcatcgcgcc ggagatctat ggccccccga agaaggaaga 16620 gcaggattac aagccccgaa agctaaagcg ggtcaaaaag aaaaagaaag atgatgatga 16680 tgaacttgac gacgaggtgg aactgctgca cgctaccgcg cccaggcgac gggtacagtg 16740 gaaaggtcga cgcgtaaaac gtgttttgcg acccggcacc accgtagtct ttacgcccgg 16800 tgagcgctcc acccgcacct acaagcgcgt gtatgatgag gtgtacggcg acgaggacct 16860 gcttgagcag gccaacgagc gcctcgggga gtttgcctac ggaaagcggc ataaggacat 16920 gctggcgttg ccgctggacg agggcaaccc aacacctagc ctaaagcccg taacactgca 16980 gcaggtgctg cccgcgcttg caccgtccga agaaaagcgc ggcctaaagc gcgagtctgg 17040 tgacttggca cccaccgtgc agctgatggt acccaagcgc cagcgactgg aagatgtctt 17100 ggaaaaaatg accgtggaac ctgggctgga gcccgaggtc cgcgtgcggc caatcaagca 17160 ggtggcgccg ggactgggcg tgcagaccgt ggacgttcag atacccacta ccagtagcac 17220 cagtattgcc accgccacag agggcatgga gacacaaacg tccccggttg cctcagcggt 17280 ggcggatgcc gcggtgcagg cggtcgctgc ggccgcgtcc aagacctcta cggaggtgca 17340 aacggacccg tggatgtttc gcgtttcagc cccccggcgc ccgcgcggtt cgaggaagta 17400 cggcgccgcc agcgcgctac tgcccgaata tgccctacat ccttccattg cgcctacccc 17460 cggctatcgt ggctacacct accgccccag aagacgagca actacccgac gccgaaccac 17520 cactggaacc cgccgccgcc gtcgccgtcg ccagcccgtg ctggccccga tttccgtgcg 17580 cagggtggct cgcgaaggag gcaggaccct ggtgctgcca acagcgcgct accaccccag 17640 catcgtttaa aagccggtct ttgtggttct tgcagatatg gccctcacct gccgcctccg 17700 tttcccggtg ccgggattcc gaggaagaat gcaccgtagg aggggcatgg ccggccacgg 17760 cctgacgggc ggcatgcgtc gtgcgcacca ccggcggcgg cgcgcgtcgc accgtcgcat 17820 gcgcggcggt atcctgcccc tccttattcc actgatcgcc gcggcgattg gcgccgtgcc 17880 cggaattgca tccgtggcct tgcaggcgca gagacactga ttaaaaacaa gttgcatgtg 17940 gaaaaatcaa aataaaaagt ctggactctc acgctcgctt ggtcctgtaa ctattttgta 18000 gaatggaaga catcaacttt gcgtctctgg ccccgcgaca cggctcgcgc ccgttcatgg 18060 gaaactggca agatatcggc accagcaata tgagcggtgg cgccttcagc tggggctcgc 18120 tgtggagcgg cattaaaaat ttcggttcca ccgttaagaa ctatggcagc aaggcctgga 18180 acagcagcac aggccagatg ctgagggata agttgaaaga gcaaaatttc caacaaaagg 18240 tggtagatgg cctggcctct ggcattagcg gggtggtgga cctggccaac caggcagtgc 18300 aaaataagat taacagtaag cttgatcccc gccctcccgt agaggagcct ccaccggccg 18360 tggagacagt gtctccagag gggcgtggcg aaaagcgtcc gcgccccgac agggaagaaa 18420 ctctggtgac gcaaatagac gagcctccct cgtacgagga ggcactaaag caaggcctgc 18480 ccaccacccg tcccatcgcg cccatggcta ccggagtgct gggccagcac acacccgtaa 18540 cgctggacct gcctcccccc gccgacaccc agcagaaacc tgtgctgcca ggcccgaccg 18600 ccgttgttgt aacccgtcct agccgcgcgt ccctgcgccg cgccgccagc ggtccgcgat 18660 cgttgcggcc cgtagccagt ggcaactggc aaagcacact gaacagcatc gtgggtctgg 18720 gggtgcaatc cctgaagcgc cgacgatgct tctgaatagc taacgtgtcg tatgtgtgtc 18780 atgtatgcgt ccatgtcgcc gccagaggag ctgctgagcc gccgcgcgcc cgctttccaa 18840 gatggctacc ccttcgatga tgccgcagtg gtcttacatg cacatctcgg gccaggacgc 18900 ctcggagtac ctgagccccg ggctggtgca gtttgcccgc gccaccgaga cgtacttcag 18960 cctgaataac aagtttagaa accccacggt ggcgcctacg cacgacgtga ccacagaccg 19020 gtcccagcgt ttgacgctgc ggttcatccc tgtggaccgt gaggatactg cgtactcgta 19080 caaggcgcgg ttcaccctag ctgtgggtga taaccgtgtg ctggacatgg cttccacgta 19140 ctttgacatc cgcggcgtgc tggacagggg ccctactttt aagccctact ctggcactgc 19200 ctacaacgcc ctggctccca agggtgcccc aaatccttgc gaatgggatg aagctgctac 19260 tgctcttgaa ataaacctag aagaagagga cgatgacaac gaagacgaag tagacgagca 19320 agctgagcag caaaaaactc acgtatttgg gcaggcgcct tattctggta taaatattac 19380 aaaggagggt attcaaatag gtgtcgaagg tcaaacacct aaatatgccg ataaaacatt 19440 tcaacctgaa cctcaaatag gagaatctca gtggtacgaa actgaaatta atcatgcagc 19500 tgggagagtc cttaaaaaga ctaccccaat gaaaccatgt tacggttcat atgcaaaacc 19560 cacaaatgaa aatggagggc aaggcattct tgtaaagcaa caaaatggaa agctagaaag 1920 tcaagtggaa atgcaatttt tctcaactac tgaggcgacc gcaggcaatg gtgataactt 1980 gactcctaaa gtggtattgt acagtgaaga tgtagatata gaaaccccag acactcatat 197440 ttcttacatg cccactatta aggaaggtaa ctcacgagaa ctaatgggcc aacaatctat 1800 gcccaacagg cctaattaca ttgcttttag ggacaatttt attggtctaa tgtattacaa 19860 cagcacgggt aatatgggtg ttctggcggg ccaagcatcg cagttgaatg ctgttgtaga 19920 tttgcaagac agaaacacag agctttcata ccagcttttg cttgattcca ttggtgatag 19980 aaccaggtac ttttctatgt ggaatcaggc tgttgacagc tatgatccag atgttagaat 20040 tattgaaaat catggaactg aagatgaact tccaaattac tgctttccac tgggaggtgt 20100 gattaataca gagactctta ccaaggtaaa acctaaaaca ggtcaggaaa atggatggga 20160 aaaagatgct acagaatttt cagataaaaa tgaaataaga gttggaaata attttgccat 20220 ggaaatcaat ctaaatgcca acctgtggag aaatttcctg tactccaaca tagcgctgta 20280 tttgcccgac aagctaaagt acagtccttc caacgtaaaa atttctgata acccaaacac 20340 ctacgactac atgaacaagc gagtggtggc tccgggtta gtggactgct acattaacct 20400 tggagcacgc tggtcccttg actatatgga caacgtcaac ccatttaacc accaccgcaa 20460 tgctggcctg cgctaccgct caatgttgct gggcaatggt cgctatgtgc ccttccacat 20520 ccaggtgcct cagaagttct ttgccattaa aaacctcctt ctcctgccgg gctcatacac 20580 ctacgagtgg aacttcagga aggatgttaa catggttctg cagagctccc taggaaatga 20640 cctaagggtt gacggagcca gcattaagtt tgatagcatt tgcctttacg ccaccttctt 20700 ccccatggcc cacaacaccg cctccacgct tgaggccatg cttagaaacg acaccaacga 20760 ccagtccttt aacgactatc tctccgccgc caacatgctc taccctatac ccgccaacgc 20820 taccaacgtg cccatatcca tcccctcccg caactgggcg gctttccgcg gctgggcctt 20880 cacgcgcctt aagactaagg aaaccccatc actgggctcg ggctacgacc cttattacac 20940 ctactctggc tctataccct acctagatgg aaccttttac ctcaaccaca cctttaagaa 21000 ggtggccatt acctttgact cttctgtcag ctggcctggc aatgaccgcc tgcttacccc 21060 caacgagttt gaaattaagc gctcagttga cggggagggt tacaacgttg cccagtgtaa 21120 catgaccaaa gactggttcc tggtacaaat gctagctaac tacaacattg gctaccaggg 21180 cttctatatc ccagagagct acaaggaccg catgtactcc ttctttagaa acttccagcc 21240 catgagccgt caggtggtgg atgatactaa atacaaggac taccaacagg tgggcatcct 21300 acaccaacac aacaactctg gatttgttgg ctaccttgcc cccaccatgc gcgaaggaca 21360 ggcctaccct gctaacttcc cctatccgct tataggcaag accgcagttg acagcattac 21420 ccagaaaaag tttctttgcg atcgcaccct ttggcgcatc ccattctcca gtaactttat 21480 gtccatgggc gcactcacag acctgggcca aaaccttctc tacgccaact ccgccccacgc 21540 gctagacatg acttttgagg tggatcccat ggacgagccc acccttcttt atgttttgtt 21600 tgaagtcttt gacgtggtcc gtgtgcaccg gccgcaccgc ggcgtcatcg aaaccgtgta 21660 cctgcgcacg cccttctcgg ccggcaacgc cacaacataa agaagcaagc aacatcaaca 21720 acagctgccg ccatgggctc cagtgagcag gaactgaaag ccattgtcaa agatcttggt 21780 tgtgggccat attttttggg cacctatgac aagcgctttc caggctttgt ttctccacac 21840 aagctcgcct gcgccatagt caatacggcc ggtcgcgaga ctgggggcgt acactggatg 21900 gcctttgcct ggaacccgca ctcaaaaaca tgctacctct ttgagccctt tggcttttct 21960 gaccagcgac tcaagcaggt ttaccagttt gagtacgagt cactcctgcg ccgtagcgcc 22020 attgcttctt cccccgaccg ctgtataacg ctggaaaagt ccacccaaag cgtacagggg 22080 cccaactcgg ccgcctgtgg actattctgc tgcatgtttc tccacgcctt tgccaactgg 22140 ccccaaactc ccatggatca caaccccacc atgaacctta ttaccggggt acccaactcc 22200 atgctcaaca gtccccaggt acagcccacc ctgcgtcgca accaggaaca gctctacagc 22260 ttcctggagc gccactcgcc ctacttccgc agccacagtg cgcagattag gagcgccact 22320 tctttttgtc acttgaaaaa catgtaaaaa taatgtacta gagacacttt caataaaggc 22380 aaatgctttt atttgtacac tctcgggtga ttatttaccc ccacccttgc cgtctgcgcc 22440 gtttaaaaat caaaggggtt ctgccgcgca tcgctatgcg ccactggcag ggacacgttg 22500 cgatactggt gtttagtgct ccacttaaac tcaggcacaa ccatccgcgg cagctcggtg 22560 aagttttcac tccacaggct gcgcaccatc accaacgcgt ttagcaggtc gggcgccgat 22620 atcttgaagt cgcagttggg gcctccgccc tgcgcgcgcg agttgcgata cacagggttg 22680 cagcactgga acactatcag cgccgggtgg tgcacgctgg ccagcacgct cttgtcggag 22740 atcagatccg cgtccaggtc ctccgcgttg ctcagggcga acggagtcaa ctttggtagc 22800 tgccttccca aaaagggcgc gtgcccaggc tttgagttgc actcgcaccg tagtggcatc 22860 aaaaggtgac cgtgcccggt ctgggcgtta ggatacagcg cctgcataaa agccttgatc 22920 tgcttaaaag ccacctgagc ctttgcgcct tcagagaaga acatgccgca agacttgccg 22980 gaaaactgat tggccggaca ggccgcgtcg tgcacgcagc accttgcgtc ggtgttggag 23040 atctgcacca catttcggcc ccaccggttc ttcacgatct tggccttgct agactgctcc 23100 ttcagcgcgc gctgcccgtt ttcgctcgtc acatccattt caatcacgtg ctccttattt 23160 atcataatgc ttccgtgtag acacttaagc tcgccttcga tctcagcgca gcggtgcagc 23220 cacaacgcgc agcccgtggg ctcgtgatgc ttgtaggtca cctctgcaaa cgactgcagg 23280 tacgcctgca ggaatcgccc catcatcgtc acaaaggtct tgttgctggt gaaggtcagc 23340 tgcaacccgc ggtgctcctc gttcagccag gtcttgcata cggccgccag agcttccact 23400 tggtcaggca gtagtttgaa gttcgccttt agatcgttat ccacgtggta cttgtccatc 23460 agcgcgcgcg cagcctccat gcccttctcc cacgcagaca cgatcggcac actcagcggg 23520 ttcatcaccg taatttcact ttccgcttcg ctgggctctt cctcttcctc ttgcgtccgc 23580 ataccacgcg ccactgggtc gtcttcattc agccgccgca ctgtgcgctt acctcctttg 23640 ccatgcttga ttagcaccgg tgggttgctg aaacccacca tttgtagcgc cacatcttct 23700 ctttcttcct cgctgtccac gattacctct ggtgatggcg ggcgctcggg cttgggagaa 23760 gggcgcttct ttttcttctt gggcgcaatg gccaaatccg ccgccgaggt cgatggccgc 23820 gggctgggtg tgcgcggcac cagcgcgtct tgtgatgagt cttcctcgtc ctcggactcg 23880 atacgccgcc tcatccgctt ttttgggggc gcccggggag gcggcggcga cggggacggg 23940 gacgacacgt cctccatggt tgggggacgt cgcgccgcac cgcgtccgcg ctcgggggtg 24000 gtttcgcgct gctcctcttc ccgactggcc atttccttct cctataggca gaaaaagatc 24060 atggagtcag tcgagaagaa ggacagccta accgccccct ctgagttcgc caccaccgcc 24120 tccaccgatg ccgccaacgc gcctaccacc ttccccgtcg aggcaccccc gcttgaggag 24180 gaggaagtga ttatcgagca ggacccaggt tttgtaagcg aagacgacga ggaccgctca 24240 gtaccaacag aggataaaaa gcaagaccag gacaacgcag aggcaaacga ggaacaagtc 24300 gggcgggggg acgaaaggca tggcgactac ctagatgtgg gagacgacgt gctgttgaag 24360 catctgcagc gccagtgcgc cattatctgc gacgcgttgc aagagcgcag cgatgtgccc 24420 ctcgccatag cggatgtcag ccttgcctac gaacgccacc tattctcacc gcgcgtaccc 24480 cccaaacgcc aagaaaacgg cacatgcgag cccaacccgc gcctcaactt ctaccccgta 24540 tttgccgtgc cagaggtgct tgccacctat cacatctttt tccaaaactg caagataccc 24600 ctatcctgcc gtgccaaccg cagccgagcg gacaagcagc tggccttgcg gcagggcgct 24660 gtcatacctg atatcgcctc gctcaacgaa gtgccaaaaa tctttgaggg tcttggacgc 24720 gacgagaagc gcgcggcaaa cgctctgcaa caggaaaaca gcgaaaatga aagtcactct 24780 ggagtgttgg tggaactcga gggtgacaac gcgcgcctag ccgtactaaa acgcagcatc 24840 gaggtcaccc actttgccta cccggcactt aacctacccc ccaaggtcat gagcacagtc 24900 atgagtgagc tgatcgtgcg ccgtgcgcag cccctggaga gggatgcaaa tttgcaagaa 24960 caaacagagg agggcctacc cgcagttggc gacgagcagc tagcgcgctg gcttcaaacg 25020 cgcgagcctg ccgacttgga ggagcgacgc aaactaatga tggccgcagt gctcgttacc 25080 gtggagcttg agtgcatgca gcggttcttt gctgacccgg agatgcagcg caagctagag 25140 gaaacattgc actacacctt tcgacagggc tacgtacgcc aggcctgcaa gatctccaac 25200 gtggagctct gcaacctggt ctcctacctt ggaattttgc acgaaaaccg ccttgggcaa 25260 aacgtgcttc attccacgct caagggcgag gcgcgccgcg actacgtccg cgactgcgtt 25320 tacttatttc tatgctacac ctggcagacg gccatgggcg tttggcagca gtgcttggag 25380 gagtgcaacc tcaaggagct gcagaaactg ctaaagcaaa acttgaagga cctatggacg 25440 gccttcaacg agcgctccgt ggccgcgcac ctggcggaca tcattttccc cgaacgcctg 25500 cttaaaaccc tgcaacaggg tctgccagac ttcaccagtc aaagcatgtt gcagaacttt 25560 aggaacttta tcctagagcg ctcaggaatc ttgcccgcca cctgctgtgc acttcctagc 25620 gactttgtgc ccattaagta ccgcgaatgc cctccgccgc tttggggcca ctgctacctt 25680 ctgcagctag ccaactacct tgcctaccac tctgacataa tggaagacgt gagcggtgac 25740 ggtctactgg agtgtcactg tcgctgcaac ctatgcaccc cgcaccgctc cctggtttgc 25800 aattcgcagc tgcttaacga aagtcaaatt atcggtacct ttgagctgca gggtccctcg 25860 cctgacgaaa agtccgcggc tccggggttg aaactcactc cggggctgtg gacgtcggct 25920 taccttcgca aatttgtacc tgaggactac cacgcccacg agattaggtt ctacgaagac 25980 caatcccgcc cgccaaatgc ggagcttacc gcctgcgtca ttacccaggg ccacattctt 26040 ggccaattgc aagccatcaa caaagcccgc caagagtttc tgctacgaaa gggacggggg 26100 gtttacttgg acccccagtc cggcgaggag ctcaacccaa tccccccgcc gccgcagccc 26160 tatcagcagc agccgcgggc ccttgcttcc caggatggca cccaaaaaga agctgcagct 26220 gccgccgcca cccacggacg aggaggaata ctgggacagt caggcagagg aggttttgga 26280 cgaggaggag gaggacatga tggaagactg ggagagccta gacgaggaag cttccgaggt 26340 cgaagaggtg tcagacgaaa caccgtcacc ctcggtcgca ttcccctcgc cggcgcccca 26400 gaaatcggca accggttcca gcatggctac aacctccgct cctcaggcgc cgccggcact 26460 gcccgttcgc cgacccaacc gtagatggga caccactgga accagggccg gtaagtccaa 26520 gcagccgccg ccgttagccc aagagcaaca acagcgccaa ggctaccgct catggcgcgg 26580 gcacaagaac gccatagttg cttgcttgca agactgtggg ggcaacatct ccttcgcccg 26640 ccgctttctt ctctaccatc acggcgtggc cttcccccgt aacatcctgc attactaccg 26700 tcatctctac agcccatact gcaccggcgg cagcggcagc ggcagcaaca gcagcggcca 26760 cacagaagca aaggcgaccg gatagcaaga ctctgacaaa gcccaagaaa tccacagcgg 26820 cggcagcagc aggaggagga gcgctgcgtc tggcgcccaa cgaacccgta tcgacccgcg 26880 agcttagaaa caggattttt cccactctgt atgctatatt tcaacagagc aggggccaag 26940 aacaagagct gaaaataaaa aacaggtctc tgcgatccct cacccgcagc tgcctgtatc 27000 acaaaagcga agatcagctt cggcgcacgc tggaagacgc ggaggctctc ttcagtaaat 27060 actgcgcgct gactcttaag gactagtttc gcgccctttc tcaaatttaa gcgcgaaaac 27120 tacgtcatct ccagcggcca cacccggcgc cagcacctgt cgtcagcgcc attatgagca 27180 aggaaattcc cacgccctac atgtggagtt accagccaca aatgggactt gcggctggag 27240 ctgcccaaga ctactcaacc cgaataaact acatgagcgc gggaccccac atgatatccc 27300 gggtcaacgg aatccgcgcc caccgaaacc gaattctctt ggaacaggcg gctattacca 27360 ccacacctcg taataacctt aatccccgta gttggcccgc tgccctggtg taccaggaaa 27420 gtcccgctcc caccactgtg gtacttccca gagacgccca ggccgaagtt cagatgacta 27480 actcaggggc gcagcttgcg ggcggctttc gtcacagggt gcggtcgccc gggcagggta 27540 taactcacct gacaatcaga gggcgaggta ttcagctcaa cgacgagtcg gtgagctcct 27600 cgcttggtct ccgtccggac gggacatttc agatcggcgg cgccggccgt ccttcattca 27660 cgcctcgtca ggcaatccta actctgcaga cctcgtcctc tgagccgcgc tctggaggca 27720 ttggaactct gcaatttatt gaggagtttg tgccatcggt ctactttaac cccttctcgg 27780 gacctcccgg ccactatccg gatcaattta ttcctaactt tgacgcggta aaggactcgg 27840 cggacggcta cgactgaatg ttaagtggag aggcagagca actgcgcctg aaacacctgg 27900 tccactgtcg ccgccacaag tgctttgccc gcgactccgg tgagttttgc tactttgaat 27960 tgcccgagga tcatatcgag ggcccggcgc acggcgtccg gcttaccgcc cagggagagc 28020 ttgcccgtag cctgattcgg gagtttaccc agcgccccct gctagttgag cgggacaggg 28080 gaccctgtgt tctcactgtg atttgcaact gtcctaacct tggattacat caagatcttt 28140 gttgccatct ctgtgctgag tataataaat acagaaatta aaatatactg gggctcctat 28200 cgccatcctg taaacgccac cgtcttcacc cgcccaagca aaccaaggcg aaccttacct 28260 ggtactttta acatctctcc ctctgtgatt tacaacagtt tcaacccaga cggagtgagt 28320 ctacgagaga acctctccga gctcagctac tccatcagaa aaaacaccac cctccttacc 28380 tgccgggaac gtacgagtgc gtcaccggcc gctgcaccac acctaccgcc tgaccgtaaa 28440 ccagactttt tccggacaga cctcaataac tctgtttacc agaacaggag gtgagcttag 28500 aaaaccctta gggtattagg ccaaaggcgc agctactgtg gggtttatga acaattcaag 28560 caactctacg ggctattcta attcaggttt ctctagaatc ggggttgggg ttattctctg 28620 tcttgtgatt ctctttattc ttatactaac gcttctctgc ctaaggctcg ccgcctgctg 28680 tgtgcacatt tgcatttatt gtcagctttt taaacgctgg ggtcgccacc caagatgatt 28740 aggtacataa tcctaggttt actcaccctt gcgtcagccc acggtaccac ccaaaaggtg 28800 gattttaagg agccagcctg taatgttaca ttcgcagctg aagctaatga gtgcaccact 28860 cttataaaat gcaccacaga acatgaaaag ctgcttattc gccacaaaaa caaaattggc 28920 aagtatgctg tttatgctat ttggcagcca ggtgacacta cagagtataa tgttacagtt 28980 ttccagggta aaagtcataa aacttttatg tatacttttc cattttatga aatgtgcgac 29040 attaccatgt acatgagcaa acagtataag ttgtggcccc cacaaaattg tgtggaaaac 29100 actggcactt tctgctgcac tgctatgcta attacagtgc tcgctttggt ctgtacccta 29160 ctctatatta aatacaaaag cagacgcagc tttattgagg aaaagaaaat gccttaattt 29220 actaagttac aaagctaatg tcaccactaa ctgctttact cgctgcttgc aaaacaaatt 29280 caaaaagtta gcattataat tagaatagga tttaaacccc ccggtcattt cctgctcaat 29340 accattcccc tgaacaattg actctatgtg ggatatgctc cagcgctaca accttgaagt 29400 caggcttcct ggatgtcagc atctgacttt ggccagcacc tgtcccgcgg atttgttcca 29460 gtccaactac agcgacccac cctaacagag atgaccaaca caaccaacgc ggccgccgct 29520 accggactta catctaccac aaatacaccc caagtttctg cctttgtcaa taactgggat 29580 aacttgggca tgtggtggtt ctccatagcg cttatgtttg tatgccttat tattatgtgg 29640 ctcatctgct gcctaaagcg caaacgcgcc cgaccaccca tctatagtcc catcattgtg 29700 ctacacccaa acaatgatgg aatccataga ttggacggac tgaaacacat gttcttttct 29760 cttacagtat gattaaatga gacatgattc ctcgagtttt tatattactg acccttgttg 29820 cgcttttttg tgcgtgctcc acattggctg cggtttctca catcgaagta gactgcattc 29880 cagccttcac agtctatttg ctttacggat ttgtcaccct cacgctcatc tgcagcctca 29940 tcactgtggt catcgccttt atccagtgca ttgactgggt ctgtgtgcgc tttgcatatc 30000 tcagacacca tccccagtac agggacagga ctatagctga gcttcttaga attctttaat 30060 tatgaaattt actgtgactt ttctgctgat tatttgcacc ctatctgcgt tttgttcccc 30120 gacctccaag cctcaaagac atatatcatg cagattcact cgtatatgga atattccaag 30180 ttgctacaat gaaaaaagcg atctttccga agcctggtta tatgcaatca tctctgttat 30240 ggtgttctgc agtaccatct tagccctagc tatatatccc taccttgaca ttggctggaa 30300 acgaatagat gccatgaacc acccaacttt ccccgcgccc gctatgcttc cactgcaaca 30360 agttgttgcc ggcggctttg tcccagccaa tcagcctcgc cccacttctc ccaccccccac 30420 tgaaatcagc tactttaatc taacaggagg agatgactga caccctagat ctagaaatgg 30480 acggaattat tacagagcag cgcctgctag aaagacgcag ggcagcggcc gagcaacagc 30540 gcatgaatca agagctccaa gacatggtta acttgcacca gtgcaaaagg ggtatctttt 30600 gtctggtaaa gcaggccaaa gtcacctacg acagtaatac caccggacac cgccttagct 30660 acaagttgcc aaccaagcgt cagaaattgg tggtcatggt gggagaaaag cccattacca 30720 taactcagca ctcggtagaa accgaaggct gcattcactc accttgtcaa ggacctgagg 30780 atctctgcac ccttattaag accctgtgcg gtctcaaaga tcttattccc tttaactaat 30840 aaaaaaaaat aataaagcat cacttactta aaatcagtta gcaaatttct gtccagttta 30900 ttcagcagca cctccttgcc ctcctcccag ctctggtatt gcagcttcct cctggctgca 30960 aactttctcc acaatctaaa tggaatgtca gtttcctcct gttcctgtcc atccgcaccc 31020 actatcttca tgttgttgca gatgaagcgc gcaagaccgt ctgaagatac cttcaacccc 31080 gtgtatccat atgacacgga aaccggtcct ccaactgtgc cttttcttac tcctcccttt 31140 gtatccccca atgggtttca agagagtccc cctggggtac tctctttgcg cctatccgaa 31200 cctctagtta cctccaatgg catgcttgcg ctcaaaatgg gcaacggcct ctctctggac 31260 gaggccggca accttacctc ccaaaatgta accactgtga gcccacctct caaaaaaacc 31320 aagtcaaaca taaacctgga aatatctgca cccctcacag ttacctcaga agccctaact 31380 gtggctgccg ccgcacctct aatggtcgcg ggcaacacac tcaccatgca atcacaggcc 31440 ccgctaaccg tgcacgactc caaacttagc attgccaccc aaggacccct cacagtgtca 31500 gaaggaaagc tagccctgca aacatcaggc cccctcacca ccaccgatag cagtaccctt 31560 actatcactg cctcaccccc tctaactact gccactggta gcttgggcat tgacttgaaa 31620 gagcccattt atacacaaaa tggaaaacta ggactaaagt acggggctcc tttgcatgta 31680 acagacgacc taaacacttt gaccgtagca actggtccag gtgtgactat taataatact 31740 tccttgcaaa ctaaagttac tggagccttg ggttttgatt cacaaggcaa tatgcaactt 31800 aatgtagcag gaggactaag gattgattct caaaacagac gccttatact tgatgttagt 31860 tatccgtttg atgctcaaaa ccaactaaat ctaagactag gacagggccc tctttttata 31920 aactcagccc acaacttgga tattaactac aacaaaggcc tttacttgtt tacagcttca 31980 aacaattcca aaaagcttga ggttaaccta agcactgcca aggggttgat gtttgacgct 32040 acagccatag ccattaatgc aggagatggg cttgaatttg gttcacctaa tgcaccaaac 32100 acaaatcccc tcaaaacaaa aattggccat ggcctagaat ttgattcaaa caaggctatg 32160 gttcctaaac taggaactgg ccttagtttt gacagcacag gtgccattac agtaggaaac 32220 aaaaataatg ataagctaac tttgtggacc acaccagctc catctcctaa ctgtagacta 32280 aatgcagaga aagatgctaa actcactttg gtcttaacaa aatgtggcag tcaaatactt 32340 gctacagttt cagttttggc tgttaaaggc agtttggctc caatatctgg aacagttcaa 32400 agtgctcatc ttattataag atttgacgaa aatggagtgc tactaaacaa ttccttcctg 32460 gacccagaat attggaactt tagaaatgga gatcttactg aaggcacagc ctatacaaac 32520 gctgttggat ttatgcctaa cctatcagct tatccaaaat ctcacggtaa aactgccaaa 32580 agtaacattg tcagtcaagt tacttaaac ggagacaaaa ctaaacctgt aacactaacc 32640 attacactaa acggtacaca ggaaacagga gacacaactc caagtgcata ctctatgtca 32700 ttttcatggg actggtctgg ccacaactac attaatgaaa tatttgccac atcctcttac 32760 actttttcat acattgccca agaataaaga atcgtttgtg ttatgtttca acgtgtttat 32820 ttttcaattg cagaaaattt caagtcattt ttcattcagt agtatagccc caccaccaca 32880 tagcttatac agatcaccgt accttaatca aactcacaga accctagtat tcaacctgcc 32940 acctccctcc caacacacag agtacacagt cctttctccc cggctggcct taaaaagcat 33000 catatcatgg gtaacagaca tattcttagg tgttatattc cacacggttt cctgtcgagc 33060 caaacgctca tcagtgatat taataaactc cccgggcagc tcacttaagt tcatgtcgct 33120 gtccagctgc tgagccacag gctgctgtcc aacttgcggt tgcttaacgg gcggcgaagg 33180 agaagtccac gcctacatgg gggtagagtc ataatcgtgc atcaggatag ggcggtggtg 33240 ctgcagcagc gcgcgaataa actgctgccg ccgccgctcc gtcctgcagg aatacaacat 33300 ggcagtggtc tcctcagcga tgattcgcac cgcccgcagc ataaggcgcc ttgtcctccg 33360 ggcacagcag cgcaccctga tctcacttaa atcagcacag taactgcagc acagcaccac 33420 aatattgttc aaaatcccac agtgcaaggc gctgtatcca aagctcatgg cggggaccac 33480 agaacccacg tggccatcat accacaagcg caggtagatt aagtggcgac ccctcataaa 33540 cacgctggac ataaacatta cctcttttgg catgttgtaa ttcaccacct cccggtacca 33600 tataaacctc tgattaaaca tggcgccatc caccaccatc ctaaaccagc tggccaaaac 33660 ctgcccgccg gctatacact gcagggaacc gggactggaa caatgacagt ggagagccca 33720 ggactcgtaa ccatggatca tcatgctcgt catgatatca atgttggcac aacacaggca 33780 cacgtgcata cacttcctca ggattacaag ctcctcccgc gttagaacca tatcccaggg 33840 aacaacccat tcctgaatca gcgtaaatcc cacactgcag ggaagacctc gcacgtaact 33900 cacgttgtgc attgtcaaag tgttacattc gggcagcagc ggatgatcct ccagtatggt 33960 agcgcgggtt tctgtctcaa aaggaggtag acgatcccta ctgtacggag tgcgccgaga 34020 caaccgagat cgtgttggtc gtagtgtcat gccaaatgga acgccggacg tagtcatatt 34080 tcctgaagca aaaccaggtg cgggcgtgac aaacagatct gcgtctccgg tctcgccgct 34140 tagatcgctc tgtgtagtag ttgtagtata tccactctct caaagcatcc aggcgccccc 34200 tggcttcggg ttctatgtaa actccttcat gcgccgctgc cctgataaca tccaccaccg 34260 cagaataagc cacacccagc caacctacac attcgttctg cgagtcacac acgggaggag 34320 cgggaagagc tggaagaacc atgttttttt ttttattcca aaagattatc caaaacctca 34380 aaatgaagat ctattagtg aacgcgctcc cctccggtgg cgtggtcaaa ctctacagcc 34440 aaagaacaga taatggcatt tgtaagatgt tgcacaatgg cttccaaaag gcaaacggcc 34500 ctcacgtcca agtggacgta aaggctaaac ccttcagggt gaatctcctc tataaacatt 34560 ccagcacctt caaccatgcc caaataattc tcatctcgcc accttctcaa tatatctcta 34620 agcaaatccc gaatattaag tccggccatt gtaaaaatct gctccagagc gccctccacc 34680 ttcagcctca agcagcgaat catgattgca aaaattcagg ttcctcacag acctgtataa 34740 gattcaaaag cggaacatta acaaaaatac cgcgatcccg taggtccctt cgcagggcca 34800 gctgaacata atcgtgcagg tctgcacgga ccagcgcggc cacttccccg ccaggaacca 34860 tgacaaaaga acccacactg attatgacac gcatactcgg agctatgcta accagcgtag 34920 ccccgatgta agcttgttgc atgggcggcg atataaaatg caaggtgctg ctcaaaaaat 34980 caggcaaagc ctcgcgcaaa aaagaaagca catcgtagtc atgctcatgc agataaaggc 35040 aggtaagctc cggaaccacc acagaaaaag acaccatttt tctctcaaac atgtctgcgg 35100 gtttctgcat aaacacaaaa taaaataaca aaaaaacatt taaacattag aagcctgtct 35160 tacaacagga aaaacaaccc ttataagcat aagacggact acggccatgc cggcgtgacc 35220 gtaaaaaaac tggtcaccgt gattaaaaag caccaccgac agctcctcgg tcatgtccgg 35280 agtcataatg taagactcgg taaacacatc aggttgattc acatcggtca gtgctaaaaa 35340 gcgaccgaaa tagcccgggg gaatacatac ccgcaggcgt agagacaaca ttacagcccc 35400 cataggaggt ataacaaaat taataggaga gaaaaacaca taaacacctg aaaaaccctc 35460 ctgcctaggc aaaatagcac cctcccgctc cagaacaaca tacagcgctt ccacagcggc 35520 agccataaca gtcagcctta ccagtaaaaa agaaaaccta ttaaaaaaac accactcgac 35580 acggcaccag ctcaatcagt cacagtgtaa aaaagggcca agtgcagagc gagtatatat 35640 aggactaaaa aatgacgtaa cggttaaagt ccacaaaaaa cacccagaaa accgcacgcg 35700 aacctacgcc cagaaacgaa agccaaaaaa cccacaactt cctcaaatcg tcacttccgt 35760 tttcccacgt tacgtaactt cccattttaa gaaaactaca attcccaaca catacaagtt 35820 actccgccct aaaacctacg tcacccgccc cgttcccacg ccccgcgcca cgtcacaaac 35880 tccaccccct cattatcata ttggcttcaa tccaaaataa ggtatattat tgatgatg 35938 <210> 35 <211> 35931 <212> DNA <213> Homo sapiens <400> 35 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagatgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccat gagcaccaac tcgtttgatg gaagcattgt gagctcatat ttgacaacgc 3660 gcatgccccc atgggccggg gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc 3720 ccgtcctgcc cgcaaactct actaccttga cctacgagac cgtgtctgga acgccgttgg 3780 agactgcagc ctccgccgcc gcttcagccg ctgcagccac cgcccgcggg attgtgactg 3840 actttgcttt cctgagcccg cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg 3900 acaagttgac ggctcttttg gcacaattgg attctttgac ccgggaactt aatgtcgttt 3960 ctcagcagct gttggatctg cgccagcagg tttctgccct gaaggcttcc tcccctccca 4020 atgcggttta aaacataaat aaaaaaccag actctgtttg gatttggatc aagcaagtgt 4080 cttgctgtct ttatttaggg gttttgcgcg cgcggtaggc ccgggaccag cggtctcggt 4140 cgttgagggt cctgtgtatt ttttccagga cgtggtaaag gtgactctgg atgttcagat 4200 acatgggcat aagcccgtct ctggggtgga ggtagcacca ctgcagagct tcatgctgcg 4260 gggtggtgtt gtagatgatc cagtcgtagc aggagcgctg ggcgtggtgc ctaaaaatgt 4320 ctttcagtag caagctgatt gccaggggca ggcccttggt gtaagtgttt acaaagcggt 4380 taagctggga tgggtgcata cgtggggata tgagatgcat cttggactgt atttttaggt 4440 tggctatgtt cccagccata tccctccggg gattcatgtt gtgcagaacc accagcacag 4500 tgtatccggt gcacttggga aatttgtcat gtagcttaga aggaaatgcg tggaagaact 4560 tggagacgcc cttgtgacct ccaagatttt ccatgcattc gtccataatg atggcaatgg 4620 gcccacgggc ggcggcctgg gcgaagatat ttctgggatc actaacgtca tagttgtgtt 4680 ccaggatgag atcgtcatag gccattttta caaagcgcgg gcggagggtg ccagactgcg 4740 gtataatggt tccatccggc ccaggggcgt agttaccctc acagatttgc atttccccacg 4800 ctttgagttc agatgggggg atcatgtcta cctgcggggc gatgaagaaa acggtttccg 4860 gggtagggga gatcagctgg gaagaaagca ggttcctgag cagctgcgac ttaccgcagc 4920 cggtgggccc gtaaatcaca cctattaccg gctgcaactg gtagttaaga gagctgcagc 4980 tgccgtcatc cctgagcagg ggggccactt cgttaagcat gtccctgact cgcatgtttt 5040 ccctgaccaa atccgccaga aggcgctcgc cgcccagcga tagcagttct tgcaaggaag 5100 caaagttttt caacggtttg agaccgtccg ccgtaggcat gcttttgagc gtttgaccaa 5160 gcagttccag gcggtcccac agctcggtca cctgctctac ggcatctcga tccagcatat 5220 ctcctcgttt cgcgggttgg ggcggctttc gctgtacggc agtagtcggt gctcgtccag 5280 acgggccagg gtcatgtctt tccacgggcg cagggtcctc gtcagcgtag tctgggtcac 5340 ggtgaagggg tgcgctccgg gctgcgcgct ggccagggtg cgcttgaggc tggtcctgct 5400 ggtgctgaag cgctgccggt cttcgccctg cgcgtcggcc aggtagcatt tgaccatggt 5460 gtcatagtcc agcccctccg cggcgtggcc cttggcgcgc agcttgccct tggaggaggc 5520 gccgcacgag gggcagtgca gacttttgag ggcgtagagc ttgggcgcga gaaataccga 5580 ttccggggag taggcatccg cgccgcaggc cccgcagacg gtctcgcatt ccacgagcca 5640 ggtgagctct ggccgttcgg ggtcaaaaac caggtttccc ccatgctttt tgatgcgttt 5700 cttacctctg gtttccatga gccggtgtcc acgctcggtg acgaaaaggc tgtccgtgtc 5760 cccgtataca gacttgagag gcctgtcctc gagcggtgtt ccgcggtcct cctcgtatag 5820 aaactcggac cactctgaga caaaggctcg cgtccaggcc agcacgaagg aggctaagtg 5880 ggaggggtag cggtcgttgt ccactagggg gtccactcgc tccagggtgt gaagacacat 5940 gtcgccctct tcggcatcaa ggaaggtgat tggtttgtag gtgtaggcca cgtgaccggg 6000 tgttcctgaa ggggggctat aaaagggggt gggggcgcgt tcgtcctcac tctcttccgc 6060 atcgctgtct gcgagggcca gctgttgggg tgagtactcc ctctgaaaag cgggcatgac 6120 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccgc 6180 ggtgatgcct ttgagggtgg ccgcatccat ctggtcagaa aagacaatct ttttgttgtc 6240 aagcttggtg gcaaacgacc cgtagagggc gttggacagc aacttggcga tggagcgcag 6300 ggtttggttt ttgtcgcgat cggcgcgctc cttggccgcg atgtttagct gcacgtattc 6360 gcgcgcaacg caccgccatt cgggaaagac ggtggtgcgc tcgtcgggca ccaggtgcac 6420 gcgccaaccg cggttgtgca gggtgacaag gtcaacgctg gtggctacct ctccgcgtag 6480 gcgctcgttg gtccagcaga ggcggccgcc cttgcgcgag cagaatggcg gtagggggtc 6540 tagctgcgtc tcgtccgggg ggtctgcgtc cacggtaaag accccgggca gcaggcgcgc 6600 gtcgaagtag tctatcttgc atccttgcaa gtctagcgcc tgctgccatg cgcgggcggc 6660 aagcgcgcgc tcgtatgggt tgagtggggg accccatggc atggggtggg tgagcgcgga 6720 ggcgtacat ccgcaaatgt cgtaaacgta gaggggctct ctgagtattc caagatatgt 6780 agggtagcat cttccaccgc ggatgctggc gcgcacgtaa tcgtatagtt cgtgcgaggg 6840 agcgaggagg tcgggaccga ggttgctacg ggcgggctgc tctgctcgga agactatctg 6900 cctgaagatg gcatgtgagt tggatgatat ggttggacgc tggaagacgt tgaagctggc 6960 gtctgtgaga cctaccgcgt cacgcacgaa ggaggcgtag gagtcgcgca gcttgttgac 7020 cagctcggcg gtgacctgca cgtctagggc gcagtagtcc agggtttcct tgatgatgtc 7080 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 7140 tttccagtac tcttggatcg gaaacccgtc ggcctccgaa cggtaagagc ctagcatgta 7200 gaactggttg acggcctggt aggcgcagca tcccttttct acgggtagcg cgtatgcctg 7260 cgcggccttc cggagcgagg tgtgggtgag cgcaaaggtg tccctgacca tgactttgag 7320 gtactggtat ttgaagtcag tgtcgtcgca tccgccctgc tcccagagca aaaagtccgt 7380 gcgctttttg gaacgcggat ttggcagggc gaaggtgaca tcgttgaaga gtatctttcc 7440 cgcgcgaggc ataaagttgc gtgtgatgcg gaagggtccc ggcacctcgg aacggttgtt 7500 aattacctgg gcggcgagca cgatctcgtc aaagccgttg atgttgtggc ccacaatgta 7560 aagttccaag aagcgcggga tgcccttgat ggaaggcaat tttttaagtt cctcgtaggt 7620 gagctcttca ggggagctga gcccgtgctc tgaaagggcc cagtctgcaa gatgagggtt 7680 ggaagcgacg aatgagctcc acaggtcacg ggccattagc atttgcaggt ggtcgcgaaa 7740 ggtcctaaac tggcgaccta tggccatttt ttctggggtg atgcagtaga aggtaagcgg 7800 gtcttgttcc cagcggtccc atccaaggtt cgcggctagg tctcgcgcgg cagtcactag 7860 aggctcatct ccgccgaact tcatgaccag catgaagggc acgagctgct tcccaaaggc 7920 ccccatccaa gtataggtct ctacatcgta ggtgacaaag agacgctcgg tgcgaggatg 7980 cgagccgatc gggaagaact ggatctcccg ccaccaattg gaggagtggc tattgatgtg 8040 gtgaaagtag aagtccctgc gacgggccga acactcgtgc tggcttttgt aaaaacgtgc 8100 gcagtactgg cagcggtgca cgggctgtac atcctgcacg aggttgacct gacgaccgcg 8160 cacaaggaag cagagtggga atttgagccc ctcgcctggc gggtttggct ggtggtcttc 8220 tacttcggct gcttgtcctt gaccgtctgg ctgctcgagg ggagttacgg tggatcggac 8280 caccacgccg cgcgagccca aagtccagat gtccgcgcgc ggcggtcgga gcttgatgac 8340 aacatcgcgc agatgggagc tgtccatggt ctggagctcc cgcggcgtca ggtcaggcgg 8400 gagctcctgc aggtttacct cgcatagacg ggtcagggcg cgggctagat ccaggtgata 8460 cctaatttcc aggggctggt tggtggcggc gtcgatggct tgcaagaggc cgcatccccg 8520 cggcgcgact acggtaccgc gcggcgggcg gtgggccgcg ggggtgtcct tggatgatgc 8580 atctaaaagc ggtgacgcgg gcgagccccc ggaggtaggg ggggctccgg acccgccggg 8640 agagggggca ggggcacgtc ggcgccgcgc gcgggcagga gctggtgctg cgcgcgtagg 8700 ttgctggcga acgcgacgac gcggcggttg atctcctgaa tctggcgcct ctgcgtgaag 8760 acgacgggcc cggtgagctt gaacctgaaa gagagttcga cagaatcaat ttcggtgtcg 8820 ttgacggcgg cctggcgcaa aatctcctgc acgtctcctg agttgtcttg ataggcgatc 8880 tcggccatga actgctcgat ctcttcctcc tggagatctc cgcgtccggc tcgctccacg 8940 gtggcggcga ggtcgttgga aatgcgggcc atgagctgcg agaaggcgtt gaggcctccc 9000 tcgttccaga cgcggctgta gaccacgccc ccttcggcat cgcgggcgcg catgaccacc 9060 tgcgcgagat tgagctccac gtgccgggcg aagacggcgt agtttcgcag gcgctgaaag 9120 aggtagttga gggtggtggc ggtgtgttct gccacgaaga agtacataac ccagcgtcgc 9180 aacgtggatt cgttgatatc ccccaaggcc tcaaggcgct ccatggcctc gtagaagtcc 9240 acggcgaagt tgaaaaactg ggagttgcgc gccgacacgg ttaactcctc ctccagaaga 9300 cggatgagct cggcgacagt gtcgcgcacc tcgcgctcaa aggctacagg ggcctcttct 9360 tcttcttcaa tctcctcttc cataagggcc tccccttctt cttcttctgg cggcggtggg 9420 ggagggggga cacggcggcg acgacggcgc accgggaggc ggtcgacaaa gcgctcgatc 9480 atctccccgc ggcgacggcg catggtctcg gtgacggcgc ggccgttctc gcgggggcgc 9540 agttggaaga cgccgcccgt catgtcccgg ttatgggttg gcggggggct gccatgcggc 9600 agggatacgg cgctaacgat gcatctcaac aattgttgtg taggtactcc gccgccgagg 9660 gacctgagcg agtccgcatc gaccggatcg gaaaacctct cgagaaaggc gtctaaccag 9720 tcacagtcgc aaggtaggct gagcaccgtg gcgggcggca gcgggcggcg gtcggggttg 9780 tttctggcgg aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag acggcggatg 9840 gtcgacagaa gcaccatgtc cttgggtccg gcctgctgaa tgcgcaggcg gtcggccatg 9900 ccccaggctt cgttttgaca tcggcgcagg tctttgtagt agtcttgcat gagcctttct 9960 accggcactt cttcttctcc ttcctcttgt cctgcatctc ttgcatctat cgctgcggcg 10020 gcggcggagt ttggccgtag gtggcgccct cttcctccca tgcgtgtgac cccgaagccc 10080 ctcatcggct gaagcagggc taggtcggcg acaacgcgct cggctaatat ggcctgctgc 10140 acctgcgtga gggtagactg gaagtcatcc atgtccacaa agcggtggta tgcgcccgtg 10200 ttgatggtgt aagtgcagtt ggccataacg gaccagttaa cggtctggtg acccggctgc 10260 gagagctcgg tgtacctgag acgcgagtaa gccctcgagt caaatacgta gtcgttgcaa 10320 gtccgcacca ggtactggta tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc 10380 cagcgtaggg tggccggggc tccgggggcg agatcttcca acataaggcg atgatatccg 10440 tagatgtacc tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg 10500 cggacgcggt tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg 10560 ccggtcaggc gcgcgcaatc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg 10620 ggcactcttc cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt 10680 tcgagccccg tatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc 10740 caggtgtgcg acgtcagaca acgggggagt gctccttttg gcttccttcc aggcgcggcg 10800 gctgctgcgc tagctttttt ggccactggc cgcgcgcagc gtaagcggtt aggctggaaa 10860 gcgaaagcat taagtggctc gctccctgta gccggagggt tattttccaa gggttgagtc 10920 gcgggacccc cggttcgagt ctcggaccgg ccggactgcg gcgaacgggg gtttgcctcc 10980 ccgtcatgca agaccccgct tgcaaattcc tccggaaaca gggacgagcc ccttttttgc 11040 ttttcccaga tgcatccggt gctgcggcag atgcgccccc ctcctcagca gcggcaagag 11100 caagagcagc ggcagacatg cagggcaccc tcccctcctc ctaccgcgtc aggaggggcg 11160 acatccgcgg ttgacgcggc agcagatggt gattacgaac ccccgcggcg ccgggcccgg 11220 cactacctgg acttggagga gggcgagggc ctggcgcggc taggagcgcc ctctcctgag 11280 cggcacccaa gggtgcagct gaagcgtgat acgcgtgagg cgtacgtgcc gcggcagaac 11340 ctgtttcgcg accgcgaggg agaggagccc gaggagatgc gggatcgaaa gttccacgca 11400 gggcgcgagc tgcggcatgg cctgaatcgc gagcggttgc tgcgcgagga ggactttgag 11460 cccgacgcgc gaaccgggat tagtcccgcg cgcgcacacg tggcggccgc cgacctggta 11520 accgcatacg agcagacggt gaaccaggag attaactttc aaaaaagctt taacaaccac 11580 gtgcgtacgc ttgtggcgcg cgaggaggtg gctataggac tgatgcatct gtgggacttt 11640 gtaagcgcgc tggagcaaaa cccaaatagc aagccgctca tggcgcagct gttccttata 11700 gtgcagcaca gcagggacaa cgaggcattc agggatgcgc tgctaaacat agtagagccc 11760 gagggccgct ggctgctcga tttgataaac atcctgcaga gcatagtggt gcaggagcgc 11820 agcttgagcc tggctgacaa ggtggccgcc atcaactatt ccatgcttag cctgggcaag 11880 ttttacgccc gcaagatata ccatacccct tacgttccca tagacaagga ggtaaagatc 11940 gaggggttct acatgcgcat ggcgctgaag gtgcttacct tgagcgacga cctgggcgtt 12000 tatcgcaacg agcgcatcca caaggccgtg agcgtgagcc ggcggcgcga gctcagcgac 12060 cgcgagctga tgcacagcct gcaaagggcc ctggctggca cgggcagcgg cgatagagag 12120 gccgagtcct actttgacgc gggcgctgac ctgcgctggg ccccaagccg acgcgccctg 12180 gaggcagctg gggccggacc tgggctggcg gtggcacccg cgcgcgctgg caacgtcggc 12240 ggcgtggagg aatatgacga ggacgatgag tacgagccag aggacggcga gtactaagcg 12300 gtgatgtttc tgatcagatg atgcaagacg caacggaccc ggcggtgcgg gcggcgctgc 12360 agagccagcc gtccggcctt aactccacgg acgactggcg ccaggtcatg gaccgcatca 12420 tgtcgctgac tgcgcgcaat cctgacgcgt tccggcagca gccgcaggcc aaccggctct 12480 ccgcaattct ggaagcggtg gtcccggcgc gcgcaaaccc cacgcacgag aaggtgctgg 12540 cgatcgtaaa cgcgctggcc gaaaacaggg ccatccggcc cgacgaggcc ggcctggtct 12600 acgacgcgct gcttcagcgc gtggctcgtt acaacagcgg caacgtgcag accaacctgg 12660 accggctggt gggggatgtg cgcgaggccg tggcgcagcg tgagcgcgcg cagcagcagg 12720 gcaacctggg ctccatggtt gcactaaacg ccttcctgag tacacagccc gccaacgtgc 12780 cgcggggaca ggaggactac accaactttg tgagcgcact gcggctaatg gtgactgaga 12840 caccgcaaag tgaggtgtac cagtctgggc cagactattt tttccagacc agtagacaag 12900 gcctgcagac cgtaaacctg agccaggctt tcaaaaactt gcaggggctg tggggggtgc 12960 gggctcccac aggcgaccgc gcgaccgtgt ctagcttgct gacgcccaac tcgcgcctgt 13020 tgctgctgct aatagcgccc ttcacggaca gtggcagcgt gtcccgggac acatacctag 13080 gtcacttgct gacactgtac cgcgaggcca taggtcaggc gcatgtggac gagcatactt 13140 tccaggagat tacaagtgtc agccgcgcgc tggggcagga ggacacgggc agcctggagg 13200 caaccctaaa ctacctgctg accaaccggc ggcagaagat cccctcgttg cacagtttaa 13260 acagcgagga ggagcgcatt ttgcgctacg tgcagcagag cgtgagcctt aacctgatgc 13320 gcgacggggt aacgcccagc gtggcgctgg acatgaccgc gcgcaacatg gaaccgggca 13380 tgtatgcctc aaaccggccg tttatcaacc gcctaatgga ctacttgcat cgcgcggccg 13440 ccgtgaaccc cgagtatttc accaatgcca tcttgaaccc gcactggcta ccgccccctg 13500 gtttctacac cgggggattc gaggtgcccg agggtaacga tggattcctc tgggacgaca 13560 tagacgacag cgtgttttcc ccgcaaccgc agaccctgct agagttgcaa cagcgcgagc 13620 aggcagaggc ggcgctgcga aaggaaagct tccgcaggcc aagcagcttg tccgatctag 13680 gcgctgcggc cccgcggtca gatgctagta gcccatttcc aagcttgata gggtctctta 13740 ccagcactcg caccacccgc ccgcgcctgc tgggcgagga ggagtaccta aacaactcgc 13800 tgctgcagcc gcagcgcgaa aaaaacctgc ctccggcatt tcccaacaac gggatagaga 13860 gcctagtgga caagatgagt agatggaaga cgtacgcgca ggagcacagg gacgtgccag 13920 gcccgcgccc gcccacccgt cgtcaaaggc acgaccgtca gcggggtctg gtgtgggagg 13980 acgatgactc ggcagacgac agcagcgtcc tggatttggg agggagtggc aacccgtttg 14040 cgcaccttcg ccccaggctg gggagaatgt tttaaaaaaa aaaaagcatg atgcaaaata 14100 aaaaactcac caaggccatg gcaccgagcg ttggttttct tgtattcccc ttagtatgcg 14160 gcgcgcggcg atgtatgagg aaggtcctcc tccctcctac gagagtgtgg tgagcgcggc 14220 gccagtggcg gcggcgctgg gttctccctt cgatgctccc ctggacccgc cgtttgtgcc 14280 tccgcggtac ctgcggccta ccggggggag aaacagcatc cgttactctg agttggcacc 14340 cctattcgac accacccgtg tgtacctggt ggacaacaag tcaacggatg tggcatccct 14400 gaactaccag aacgaccaca gcaactttct gaccacggtc attcaaaaca atgactacag 14460 cccgggggag gcaagcacac agaccatcaa tcttgacgac cggtcgcact ggggcggcga 14520 cctgaaaacc atcctgcata ccaacatgcc aaatgtgaac gagttcatgt ttaccaataa 14580 gtttaaggcg cgggtgatgg tgtcgcgctt gcctactaag gacaatcagg tggagctgaa 14640 atacgagtgg gtggagttca cgctgcccga gggcaactac tccgagacca tgaccataga 14700 ccttatgaac aacgcgatcg tggagcacta cttgaaagtg ggcagacaga acggggttct 14760 ggaaagcgac atcggggtaa agtttgacac ccgcaacttc agactggggt ttgaccccgt 14820 cactggtctt gtcatgcctg gggtatatac aaacgaagcc ttccatccag acatcatttt 14880 gctgccagga tgcggggtgg acttcaccca cagccgcctg agcaacttgt tgggcatccg 14940 caagcggcaa cccttccagg agggctttag gatcacctac gatgatctgg agggtggtaa 15000 cattcccgca ctgttggatg tggacgccta ccaggcgagc ttgaaagatg acaccgaaca 15060 gggcgggggt ggcgcaggcg gcagcaacag cagtggcagc ggcgcggaag agaactccaa 15120 cgcggcagcc gcggcaatgc agccggtgga ggacatgaac gatcatgcca ttcgcggcga 15180 cacctttgcc acacgggctg aggagaagcg cgctgaggcc gaagcagcgg ccgaagctgc 15240 cgccccccgct gcgcaacccg aggtcgagaa gcctcagaag aaaccggtga tcaaacccct 15300 gacagaggac agcaagaaac gcagttacaa cctaataagc aatgacagca ccttcaccca 15360 gtaccgcagc tggtaccttg catacaacta cggcgaccct cagaccggaa tccgctcatg 15420 gaccctgctt tgcactcctg acgtaacctg cggctcggag caggtctact ggtcgttgcc 15480 agacatgatg caagaccccg tgaccttccg ctccacgcgc cagatcagca actttccggt 15540 ggtgggcgcc gagctgttgc ccgtgcactc caagagcttc tacaacgacc aggccgtcta 15600 ctcccaactc atccgccagt ttacctctct gacccacgtg ttcaatcgct ttcccgagaa 15660 ccagattttg gcgcgcccgc cagcccccac catcaccacc gtcagtgaaa acgttcctgc 15720 tctcacagat cacgggacgc taccgctgcg caacagcatc ggaggagtcc agcgagtgac 15780 cattactgac gccagacgcc gcacctgccc ctacgtttac aaggccctgg gcatagtctc 15840 gccgcgcgtc ctatcgagcc gcactttttg agcaagcatg tccatcctta tatcgcccag 15900 caataacaca ggctggggcc tgcgcttccc aagcaagatg tttggcgggg ccaagaagcg 15960 ctccgaccaa cacccagtgc gcgtgcgcgg gcactaccgc gcgccctggg gcgcgcacaa 16020 acgcggccgc actgggcgca ccaccgtcga tgacgccatc gacgcggtgg tggaggaggc 16080 gcgcaactac acgcccacgc cgccaccagt gtccacagtg gacgcggcca ttcagaccgt 16140 ggtgcgcgga gcccggcgct atgctaaaat gaagagacgg cggaggcgcg tagcacgtcg 16200 ccaccgccgc cgacccggca ctgccgccca acgcgcggcg gcggccctgc ttaaccgcgc 16260 acgtcgcacc ggccgacggg cggccatgcg ggccgctcga aggctggccg cgggtattgt 16320 cactgtgccc cccaggtcca ggcgacgagc ggccgccgca gcagccgcgg ccattagtgc 16380 tatgactcag ggtcgcaggg gcaacgtgta ttgggtgcgc gactcggtta gcggcctgcg 16440 cgtgcccgtg cgcacccgcc ccccgcgcaa ctagattgca agaaaaaact acttagactc 16500 gtactgttgt atgtatccag cggcggcggc gcgcaacgaa gctatgtcca agcgcaaaat 16560 caaagaagag atgctccagg tcatcgcgcc ggagatctat ggccccccga agaaggaaga 16620 gcaggattac aagccccgaa agctaaagcg ggtcaaaaag aaaaagaaag atgatgatga 16680 tgaacttgac gacgaggtgg aactgctgca cgctaccgcg cccaggcgac gggtacagtg 16740 gaaaggtcga cgcgtaaaac gtgttttgcg acccggcacc accgtagtct ttacgcccgg 16800 tgagcgctcc acccgcacct acaagcgcgt gtatgatgag gtgtacggcg acgaggacct 16860 gcttgagcag gccaacgagc gcctcgggga gtttgcctac ggaaagcggc ataaggacat 16920 gctggcgttg ccgctggacg agggcaaccc aacacctagc ctaaagcccg taacactgca 16980 gcaggtgctg cccgcgcttg caccgtccga agaaaagcgc ggcctaaagc gcgagtctgg 17040 tgacttggca cccaccgtgc agctgatggt acccaagcgc cagcgactgg aagatgtctt 17100 ggaaaaaatg accgtggaac ctgggctgga gcccgaggtc cgcgtgcggc caatcaagca 17160 ggtggcgccg ggactgggcg tgcagaccgt ggacgttcag atacccacta ccagtagcac 17220 cagtattgcc accgccacag agggcatgga gacacaaacg tccccggttg cctcagcggt 17280 ggcggatgcc gcggtgcagg cggtcgctgc ggccgcgtcc aagacctcta cggaggtgca 17340 aacggacccg tggatgtttc gcgtttcagc cccccggcgc ccgcgccgtt cgaggaagta 17400 cggcgccgcc agcgcgctac tgcccgaata tgccctacat ccttccattg cgcctacccc 17460 cggctatcgt ggctacacct accgccccag aagacgagca actacccgac gccgaaccac 17520 cactggaacc cgccgccgcc gtcgccgtcg ccagcccgtg ctggccccga tttccgtgcg 17580 cagggtggct cgcgaaggag gcaggaccct ggtgctgcca acagcgcgct accaccccag 17640 catcgtttaa aagccggtct ttgtggttct tgcagatatg gccctcacct gccgcctccg 17700 tttcccggtg ccgggattcc gaggaagaat gcaccgtagg aggggcatgg ccggccacgg 17760 cctgacgggc ggcatgcgtc gtgcgcacca ccggcggcgg cgcgcgtcgc accgtcgcat 17820 gcgcggcggt atcctgcccc tccttattcc actgatcgcc gcggcgattg gcgccgtgcc 17880 cggaattgca tccgtggcct tgcaggcgca gagacactga ttaaaaacaa gttgcatgtg 17940 gaaaaatcaa aataaaaagt ctggactctc acgctcgctt ggtcctgtaa ctattttgta 18000 gaatggaaga catcaacttt gcgtctctgg ccccgcgaca cggctcgcgc ccgttcatgg 18060 gaaactggca agatatcggc accagcaata tgagcggtgg cgccttcagc tggggctcgc 18120 tgtggagcgg cattaaaaat ttcggttcca ccgttaagaa ctatggcagc aaggcctgga 18180 acagcagcac aggccagatg ctgagggata agttgaaaga gcaaaatttc caacaaaagg 18240 tggtagatgg cctggcctct ggcattagcg gggtggtgga cctggccaac caggcagtgc 18300 aaaataagat taacagtaag cttgatcccc gccctcccgt agaggagcct ccaccggccg 18360 tggagacagt gtctccagag gggcgtggcg aaaagcgtcc gcgccccgac agggaagaaa 18420 ctctggtgac gcaaatagac gagcctccct cgtacgagga ggcactaaag caaggcctgc 18480 ccaccacccg tcccatcgcg cccatggcta ccggagtgct gggccagcac acacccgtaa 18540 cgctggacct gcctcccccc gccgacaccc agcagaaacc tgtgctgcca ggcccgaccg 18600 ccgttgttgt aacccgtcct agccgcgcgt ccctgcgccg cgccgccagc ggtccgcgat 18660 cgttgcggcc cgtagccagt ggcaactggc aaagcacact gaacagcatc gtgggtctgg 18720 gggtgcaatc cctgaagcgc cgacgatgct tctgatagct aacgtgtcgt atgtgtgtca 18780 tgtatgcgtc catgtcgccg ccagaggagc tgttgagccg ccgcgcgccc gctttccaag 18840 atggctaccc cttcgatgat gccgcagtgg tcttacatgc acatctcggg ccaggacgcc 18900 tcggagtacc tgagccccgg gctggtgcag tttgcccgcg ccaccgagac gtacttcagc 18960 ctgaataaca agtttagaaa ccccacggtg gcgcctacgc acgacgtgac cacagaccgg 19020 tcccagcgtt tgacgctgcg gttcatccct gtggaccgtg aggatactgc gtactcgtac 19080 aaggcgcggt tcaccctagc tgtgggtgat aaccgtgtgc tggacatggc ttccacgtac 19140 tttgacatcc gcggcgtgct ggacaggggc cctactttta agccctactc tggcactgcc 19200 tacaacgccc tggctcccaa gggtgcccca aatccttgcg aatgggatga agctgctact 19260 gctcttgaaa taaacctaga agaagaggac gatgacaacg aagacgaagt agacgagcaa 19320 gctgagcagc aaaaaactca cgtatttggg caggcgcctt attctggtat aaatattaca 19380 aaggagggta ttcaaatagg tgtcgaaggt caaacaccta aatatgccga taaaacattt 19440 caacctgaac ctcaaatagg agaatctcag tggtacgaaa cagaaattaa tcatgcagct 19500 gggagagtcc taaaaaagac taccccaatg aaaccatgtt acggttcata tgcaaaaccc 19560 acaaatgaaa atggagggca aggcattctt gtaaagcaac aaaatggaaa gctagaaagt 1920 caagtggaaa tgcaattttt ctcaactact gaggcagccg caggcaatgg tgataacttg 1980 actcctaaag tggtattgta cagtgaagat gtagatatag aaaccccaga cactcatatt 197440 tcttacatgc ccactattaa ggaaggtaac tcacgagaac taatggcca acaatctatg 1800 cccaacaggc ctaattacat tgcttttagg gacaatttta ttggtctaat gtattacaac 19860 agcaggggta atatgggtgt tctggcgggc caagcatcgc agttgaatgc tgttgtagat 19920 ttgcaagaca gaaacacaga gctttcatac cagcttttgc ttgattccat tggtgataga 19980 accaggtact tttctatgtg gaatcaggct gttgacagct atgatccaga tgttagaatt 20040 attgaaaatc atggaactga agatgaactt ccaaattact gctttccact gggaggtgtg 20100 attaatacag agactcttac caaggtaaaa cctaaaacag gtcaggaaaa tggatgggaa 20160 aaagatgcta cagaattttc agataaaaat gaaataagag ttggaaataa ttttgccatg 20220 gaaatcaatc taaatgccaa cctgtggaga aatttcctgt actccaacat agcgctgtat 20280 ttgcccgaca agctaaagta cagtccttcc aacgtaaaaa tttctgataa cccaaacacc 20340 tacgactaca tgaacaagcg agtggtggct cccgggctag tggactgcta cattaacctt 20400 ggagcacgct ggtcccttga ctatatggac aacgtcaacc catttaacca ccaccgcaat 20460 gctggcctgc gctaccgctc aatgttgctg ggcaatggtc gctatgtgcc cttccacatc 20520 caggtgcctc agaagttctt tgccattaaa aacctccttc tcctgccggg ctcatacacc 20580 tacgagtgga acttcaggaa ggatgttaac atggttctgc agagctccct aggaaatgac 20640 ctaagggttg acggagccag cattaagttt gatagcattt gcctttacgc caccttcttc 20700 cccatggccc acaacaccgc ctccacgctt gaggccatgc ttagaaacga caccaacgac 20760 cagtccttta acgactatct ctccgccgcc aacatgctct accctatacc cgccaacgct 20820 accaacgtgc ccatatccat cccctcccgc aactgggcgg ctttccgcgg ctgggccttc 20880 acgcgcctta agactaagga aaccccatca ctgggctcgg gctacgaccc ttattacacc 20940 tactctggct ctatacccta cctagatgga accttttacc tcaaccacac ctttaagaag 21000 gtggccatta cctttgactc ttctgtcagc tggcctggca atgaccgcct gcttaccccc 21060 aacgagtttg aaattaagcg ctcagttgac ggggagggtt acaacgttgc ccagtgtaac 21120 atgaccaaag actggttcct ggtacaaatg ctagctaact ataacattgg ctaccagggc 21180 ttctatatcc cagagagcta caaggaccgc atgtactcct tctttagaaa cttccagccc 21240 atgagccgtc aggtggtgga tgatactaaa tacaaggact accaacaggt gggcatccta 21300 caccaacaca acaactctgg atttgttggc taccttgccc ccaccatgcg cgaaggacag 21360 gcctaccctg ctaacttccc ctatccgctt ataggcaaga ccgcagttga cagcattacc 21420 cagaaaaagt ttctttgcga tcgcaccctt tggcgcatcc cattctccag taactttatg 21480 tccatgggcg cactcacaga cctgggccaa aaccttctct acgccaactc cgcccacgcg 21540 ctagacatga cttttgaggt ggatcccatg gacgagccca cccttcttta tgttttgttt 21600 gaagtctttg acgtggtccg tgtgcaccag ccgcaccgcg gcgtcatcga aaccgtgtac 21660 ctgcgcacgc ccttctcggc cggcaacgcc acaacataaa gaagcaagca acatcaacaa 21720 cagctgccgc catgggctcc agtgagcagg aactgaaagc cattgtcaaa gatcttggtt 21780 gtgggccata ttttttgggc acctatgaca agcgctttcc aggctttgtt tctccacaca 21840 agctcgcctg cgccatagtc aatacggccg gtcgcgagac tgggggcgta cactggatgg 21900 cctttgcctg gaacccgcac tcaaaaacat gctacctctt tgagcccttt ggcttttctg 21960 accagcgact caagcaggtt taccagtttg agtacgagtc actcctgcgc cgtagcgcca 22020 ttgcttcttc ccccgaccgc tgtataacgc tggaaaagtc cacccaaagc gtacaggggc 22080 ccaactcggc cgcctgtgga ctattctgct gcatgtttct ccacgccttt gccaactggc 22140 cccaaactcc catggatcac aaccccacca tgaaccttat taccggggta cccaactcca 22200 tgctcaacag tccccaggta cagcccaccc tgcgtcgcaa ccaggaacag ctctacagct 22260 tcctggagcg ccactcgccc tacttccgca gccacagtgc gcagattagg agcgccactt 22320 ctttttgtca cttgaaaaac atgtaaaaat aatgtactag agacactttc aataaaggca 22380 aatgctttta tttgtacact ctcgggtgat tatttacccc cacccttgcc gtctgcgccg 22440 tttaaaaatc aaaggggttc tgccgcgcat cgctatgcgc cactggcagg gacacgttgc 22500 gatactggtg tttagtgctc cacttaaact caggcacaac catccgcggc agctcggtga 22560 agttttcact ccacaggctg cgcaccatca ccaacgcgtt tagcaggtcg ggcgccgata 22620 tcttgaagtc gcagttgggg cctccgccct gcgcgcgcga gttgcgatac acagggttgc 22680 agcactggaa cactatcagc gccgggtggt gcacgctggc cagcacgctc ttgtcggaga 22740 tcagatccgc gtccaggtcc tccgcgttgc tcagggcgaa cggagtcaac tttggtagct 22800 gccttcccaa aaagggcgcg tgcccaggct ttgagttgca ctcgcaccgt agtggcatca 22860 aaaggtgacc gtgcccggtc tgggcgttag gatacagcgc ctgcataaaa gccttgatct 22920 gcttaaaagc cacctgagcc tttgcgcctt cagagaagaa catgccgcaa gacttgccgg 22980 aaaactgatt ggccggacag gccgcgtcgt gcacgcagca ccttgcgtcg gtgttggaga 23040 tctgcaccac atttcggccc caccggttct tcacgatctt ggccttgcta gactgctcct 23100 tcagcgcgcg ctgcccgttt tcgctcgtca catccatttc aatcacgtgc tccttattta 23160 tcataatgct tccgtgtaga cacttaagct cgccttcgat ctcagcgcag cggtgcagcc 23220 acaacgcgca gcccgtgggc tcgtgatgct tgtaggtcac ctctgcaaac gactgcaggt 23280 acgcctgcag gaatcgcccc atcatcgtca caaaggtctt gttgctggtg aaggtcagct 23340 gcaacccgcg gtgctcctcg ttcagccagg tcttgcatac ggccgccaga gcttccactt 23400 ggtcaggcag tagtttgaag ttcgccttta gatcgttatc cacgtggtac ttgtccatca 23460 gcgcgcgcgc agcctccatg cccttctccc acgcagacac gatcggcaca ctcagcgggt 23520 tcatcaccgt aatttcactt tccgcttcgc tgggctcttc ctcttcctct tgcgtccgca 23580 taccacgcgc cactgggtcg tcttcattca gccgccgcac tgtgcgctta cctcctttgc 23640 catgcttgat tagcaccggt gggttgctga aacccaccat ttgtagcgcc acatcttctc 23700 tttcttcctc gctgtccacg attacctctg gtgatggcgg gcgctcgggc ttgggagaag 23760 ggcgcttctt tttcttcttg ggcgcaatgg ccaaatccgc cgccgaggtc gatggccgcg 23820 ggctgggtgt gcgcggcacc agcgcgtctt gtgatgagtc ttcctcgtcc tcggactcga 23880 tacgccgcct catccgcttt tttgggggcg cccggggagg cggcggcgac ggggacgggg 23940 acgacacgtc ctccatggtt gggggacgtc gcgccgcacc gcgtccgcgc tcgggggtgg 24000 tttcgcgctg ctcctcttcc cgactggcca tttccttctc ctataggcag aaaaagatca 24060 tggagtcagt cgagaagaag gacagcctaa ccgccccctc tgagttcgcc accaccgcct 24120 ccaccgatgc cgccaacgcg cctaccacct tccccgtcga ggcacccccg cttgaggagg 24180 aggaagtgat tatcgagcag gacccaggtt ttgtaagcga agacgacgag gaccgctcag 24240 taccaacaga ggataaaaag caagaccagg acaacgcaga ggcaaacgag gaacaagtcg 24300 ggcgggggga cgaaaggcat ggcgactacc tagatgtggg agacgacgtg ctgttgaagc 24360 atctgcagcg ccagtgcgcc attatctgcg acgcgttgca agagcgcagc gatgtgcccc 24420 tcgccatagc ggatgtcagc cttgcctacg aacgccacct attctcaccg cgcgtacccc 24480 ccaaacgcca agaaaacggc acatgcgagc ccaacccgcg cctcaacttc taccccgtat 24540 ttgccgtgcc agaggtgctt gccacctatc acatcttttt ccaaaactgc aagatacccc 24600 tatcctgccg tgccaaccgc agccgagcgg acaagcagct ggccttgcgg cagggcgctg 24660 tcatacctga tatcgcctcg ctcaacgaag tgccaaaaat ctttgagggt cttggacgcg 24720 acgagaagcg cgcggcaaac gctctgcaac aggaaaacag cgaaaatgaa agtcactctg 24780 gagtgttggt ggaactcgag ggtgacaacg cgcgcctagc cgtactaaaa cgcagcatcg 24840 aggtcaccca ctttgcctac ccggcactta acctaccccc caaggtcatg agcacagtca 24900 tgagtgagct gatcgtgcgc cgtgcgcagc ccctggagag ggatgcaaat ttgcaagaac 24960 aaacagagga gggcctaccc gcagttggcg acgagcagct agcgcgctgg cttcaaacgc 25020 gcgagcctgc cgacttggag gagcgacgca aactaatgat ggccgcagtg ctcgttaccg 25080 tggagcttga gtgcatgcag cggttctttg ctgacccgga gatgcagcgc aagctagagg 25140 aaacattgca ctacaccttt cgacagggct acgtacgcca ggcctgcaag atctccaacg 25200 tggagctctg caacctggtc tcctaccttg gaattttgca cgaaaaccgc cttgggcaaa 25260 acgtgcttca ttccacgctc aagggcgagg cgcgccgcga ctacgtccgc gactgcgttt 25320 acttatttct atgctacacc tggcagacgg ccatgggcgt ttggcagcag tgcttggagg 25380 agtgcaacct caaggagctg cagaaactgc taaagcaaaa cttgaaggac ctatggacgg 25440 ccttcaacga gcgctccgtg gccgcgcacc tggcggacat cattttcccc gaacgcctgc 25500 ttaaaaccct gcaacagggt ctgccagact tcaccagtca aagcatgttg cagaacttta 25560 ggaactttat cctagagcgc tcaggaatct tgcccgccac ctgctgtgca cttcctagcg 25620 actttgtgcc cattaagtac cgcgaatgcc ctccgccgct ttggggccac tgctaccttc 25680 tgcagctagc caactacctt gcctaccact ctgacataat ggaagacgtg agcggtgacg 25740 gtctactgga gtgtcactgt cgctgcaacc tatgcacccc gcaccgctcc ctggtttgca 25800 attcgcagct gcttaacgaa agtcaaatta tcggtacctt tgagctgcag ggtccctcgc 25860 ctgacgaaaa gtccgcggct ccggggttga aactcactcc ggggctgtgg acgtcggctt 25920 accttcgcaa atttgtacct gaggactacc acgcccacga gattaggttc tacgaagacc 25980 aatcccgccc gcctaatgcg gagcttaccg cctgcgtcat tacccagggc cacattcttg 26040 gccaattgca agccatcaac aaagcccgcc aagagtttct gctacgaaag ggacgggggg 26100 tttacttgga cccccagtcc ggcgaggagc tcaacccaat ccccccgccg ccgcagccct 26160 atcagcagca gccgcgggcc cttgcttccc aggatggcac ccaaaaagaa gctgcagctg 26220 ccgccgccac ccacggacga ggaggaatac tgggacagtc aggcagagga ggttttggac 26280 gaggaggagg aggacatgat ggaagactgg gagagcctag acgaggaagc ttccgaggtc 26340 gaagaggtgt cagacgaaac accgtcaccc tcggtcgcat tcccctcgcc ggcgccccag 26400 aaatcggcaa ccggttccag catggctaca acctccgctc ctcaggcgcc gccggcactg 26460 cccgttcgcc gacccaaccg tagatgggac accactggaa ccagggccgg taagtccaag 26520 cagccgccgc cgttagccca agagcaacaa cagcgccaag gctaccgctc atggcgcggg 26580 cacaagaacg ccatagttgc ttgcttgcaa gactgtgggg gcaacatctc cttcgcccgc 26640 cgctttcttc tctaccatca cggcgtggcc ttcccccgta acatcctgca ttactaccgt 26700 catctctaca gcccatactg caccggcggc agcggcagca acagcagcgg ccacacagaa 26760 gcaaaggcga ccggatagca agactctgac aaagcccaag aaatccacag cggcggcagc 26820 agcaggagga ggagcgctgc gtctggcgcc caacgaaccc gtatcgaccc gcgagcttag 26880 aaacaggatt tttcccactc tgtatgctat atttcaacag agcaggggcc aagaacaaga 26940 gctgaaaata aaaaacaggt ctctgcgatc cctcacccgc agctgcctgt atcacaaaag 27000 cgaagatcag cttcggcgca cgctggaaga cgcggaggct ctcttcagta aatactgcgc 27060 gctgactctt aaggactagt ttcgcgccct ttctcaaatt taagcgcgaa aactacgtca 27120 tctccagcgg ccacacccgg cgccagcacc tgttgtcagc gccattatga gcaaggaaat 27180 tcccacgccc tacatgtgga gttaccagcc acaaatggga cttgcggctg gagctgccca 27240 agactactca acccgaataa actacatgag cgcgggaccc cacatgatat cccgggtcaa 27300 cggaatacgc gcccaccgaa accgaattct cctggaacag gcggctata ccaccacacc 27360 tcgtaataac cttaatcccc gtagttggcc cgctgccctg gtgtaccagg aaagtcccgc 27420 tcccaccact gtggtacttc ccagagacgc ccaggccgaa gttcagatga ctaactcagg 27480 ggcgcagctt gcgggcggct ttcgtcacag ggtgcggtcg cccgggcagg gtataactca 27540 cctgacaatc agagggcgag gtattcagct caacgacgag tcggtgagct cctcgcttgg 27600 tctccgtccg gacgggacat ttcagatcgg cggcgccggc cgctcttcat tcacgcctcg 27660 tcaggcaatc ctaactctgc agacctcgtc ctctgagccg cgctctggag gcattggaac 27720 tctgcaattt attgaggagt ttgtgccatc ggtctacttt aaccccttct cgggacctcc 27780 cggccactat ccggatcaat ttattcctaa ctttgacgcg gtaaaggact cggcggacgg 27840 ctacgactga atgttaagtg gagaggcaga gcaactgcgc ctgaaacacc tggtccactg 27900 tcgccgccac aagtgctttg cccgcgactc cggtgagttt tgctactttg aattgcccga 27960 ggatcatatc gagggcccgg cgcacggcgt ccggcttacc gcccagggag agcttgcccg 28020 tagcctgatt cgggagttta cccagcgccc cctgctagtt gagcgggaca ggggaccctg 28080 tgttctcact gtgatttgca actgtcctaa ccctggatta catcaagatc tttgttgcca 28140 tctctgtgct gagtataata aatacagaaa ttaaaatata ctggggctcc tatcgccatc 28200 ctgtaaacgc caccgtcttc acccgcccaa gcaaaccaag gcgaacctta cctggtactt 28260 ttaacatctc tccctctgtg atttacaaca gtttcaaccc agacggagtg agtctacgag 28320 agaacctctc cgagctcagc tactccatca gaaaaaacac caccctcctt acctgccggg 28380 aacgtacgag tgcgtcaccg gccgctgcac cacacctacc gcctgaccgt aaaccagact 28440 ttttccggac agacctcaat aactctgttt accagaacag gaggtgagct tagaaaaccc 28500 ttagggtatt aggccaaagg cgcagctact gtggggttta tgaacaattc aagcaactct 28560 acgggctatt ctaattcagg tttctctaga atcggggttg gggttattct ctgtcttgtg 28620 attctcttta ttcttatact aacgcttctc tgcctaaggc tcgccgcctg ctgtgtgcac 28680 atttgcattt attgtcagct ttttaaacgc tggggtcgcc acccaagatg attaggtaca 28740 taatcctagg tttactcacc cttgcgtcag cccacggtac cacccaaaag gtggatttta 28800 aggagccagc ctgtaatgtt acattcgcag ctgaagctaa tgagtgcacc actcttataa 28860 aatgcaccac agaacatgaa aagctgctta ttcgccacaa aaacaaaatt ggcaagtatg 28920 ctgtttatgc tatttggcag ccaggtgaca ctacagagta taatgttaca gttttccagg 28980 gtaaaagtca taaaactttt atgtatactt ttccatttta tgaaatgtgc gacattacca 29040 tgtacatgag caaacagtat aagttgtggc ccccacaaaa ttgtgtggaa aacactggca 29100 ctttctgctg cactgctatg ctaattacag tgctcgcttt ggtctgtacc ctactctata 29160 ttaaatacaa aagcagacgc agctttattg aggaaaagaa aatgccttaa tttactaagt 29220 tacaaagcta atgtcaccac taactgcttt actcgctgct tgcaaaacaa attcaaaaag 29280 ttagcattat aattagaata ggatttaaac cccccggtca tttcctgctc aataccattc 29340 ccctgaacaa ttgactctat gtgggatatg ctccagcgct acaaccttga agtcaggctt 29400 cctggatgtc agcatctgac tttggccagc acctgtcccg cggatttgtt ccagtccaac 29460 tacagcgacc caccctaaca gagatgacca acacaaccaa cgcggccgcc gctaccggac 29520 ttacatctac cacaaataca ccccaagttt ctgcctttgt caataactgg gataacttgg 29580 gcatgtggtg gttctccata gcgcttatgt ttgtatgcct tattattatg tggctcatct 29640 gctgcctaaa gcgcaaacgc gcccgaccac ccatctatag tcccatcatt gtgctacacc 29700 caaacaatga tggaatccat agattggacg gactgaaaca catgttcttt tctcttacag 29760 tatgattaaa tgagacatga ttcctcgagt ttttatatta ctgacccttg ttgcgctttt 29820 ttgtgcgtgc tccacattgg ctgcggtttc tcacatcgaa gtagactgca ttccagcctt 29880 cacagtctat ttgctttacg gatttgtcac cctcacgctc atctgcagcc tcatcactgt 29940 ggtcatcgcc tttatccagt gcattgactg ggtctgtgtg cgctttgcat atctcagaca 30000 ccatccccag tacagggaca ggactatagc tgagcttctt agaattcttt aattatgaaa 30060 tttactgtga cttttctgct gattatttgc accctatctg cgttttgttc cccgacctcc 30120 aagcctcaaa gacatatatc atgcagattc actcgtatat ggaatattcc aagttgctac 30180 aatgaaaaaa gcgatctttc cgaagcctgg ttatatgcaa tcatctctgt tatggtgttc 30240 tgcagtacca tcttagccct agctatatat ccctaccttg acattggctg gaacgcaata 30300 gatgccatga accacccaac tttccccgcg cccgctatgc ttccactgca acaagttgtt 30360 gccggcggct ttgtcccagc caatcagcct cgcccacctt ctcccacccc cactgaaatc 30420 agctacttta atctaacagg aggagatgac tgacacccta gatctagaaa tggacggaat 30480 tattacagag cagcgcctgc tagaaagacg cagggcagcg gccgagcaac agcgcatgaa 30540 tcaagagctc caagacatgg ttaacttgca ccagtgcaaa aggggtatct tttgtctggt 30600 aaagcaggcc aaagtcacct acgacagtaa taccaccgga caccgcctta gctacaagtt 30660 gccaaccaag cgtcagaaat tggtggtcat ggtgggagaa aagcccatta ccataactca 30720 gcactcggta gaaaccgaag gctgcattca ctcaccttgt caaggacctg aggatctctg 30780 cacccttatt aagaccctgt gcggtctcaa agatcttatt ccctttaact aataaaaaaa 30840 aataataaag catcacttac ttaaaatcag ttagcaaatt tctgtccagt ttattcagca 30900 gcacctcctt gccctcctcc cagctctggt attgcagctt cctcctggct gcaaactttc 30960 tccacaatct aaatggaatg tcagtttcct cctgttcctg tccatccgca cccactatct 31020 tcatgttgtt gcagatgaag cgcgcaagac cgtctgaaga taccttcaac cccgtgtatc 31080 catatgacac ggaaaccggt cctccaactg tgccttttct tactcctccc tttgtatccc 31140 ccaatgggtt tcaagagagt ccccctgggg tactctcttt gcgcctatcc gaacctctag 31200 ttacctccaa tggcatgctt gcgctcaaaa tgggcaacgg cctctctctg gacgaggccg 31260 gcaaccttac ctcccaaaat gtaaccactg tgagcccacc tctcaaaaaa accaagtcaa 31320 acataaacct ggaaatatct gcacccctca cagttacctc agaagcccta actgtggctg 31380 ccgccgcacc tctaatggtc gcgggcaaca cactcaccat gcaatcacag gccccgctaa 31440 ccgtgcacga ctccaaactt agcattgcca cccaaggacc cctcacagtg tcagaaggaa 31500 agctagccct gcaaacatca ggccccctca ccaccaccga tagcagtacc cttactatca 31560 ctgcctcacc ccctctaact actgccactg gtagcttggg cattgacttg aaagagccca 31620 tttatacaca aaatggaaaa ctaggactaa agtacggggc tcctttgcat gtaacagacg 31680 acctaaacac tttgaccgta gcaactggtc caggtgtgac tattaataat acttccttgc 31740 aaactaaagt tactggagcc ttgggttttg attcacaagg caatatgcaa cttaatgtag 31800 caggaggact aaggattgat tctcaaaaca gacgccttat acttgatgtt agttatccgt 31860 ttgatgctca aaaccaacta aatctaagac taggacaggg ccctcttttt ataaactcag 31920 cccacaactt ggatattaac tacaacaaag gcctttactt gtttacagct tcaaacaatt 31980 ccaaaaagct tgaggttaac ctaagcactg ccaaggggtt gatgtttgac gctacagcca 32040 tagccattaa tgcaggagat gggcttgaat ttggttcacc taatgcacca aacacaaatc 32100 ccctcaaaac aaaaattggc catggcctag aatttgattc aaacaaggct atggttccta 32160 aactaggaac tggccttagt tttgacagca caggtgccat tacagtagga aacaaaaata 32220 atgataagct aactttgtgg accacaccag ctccatctcc taactgtaga ctaaatgcag 32280 agaaagatgc taaactcact ttggtcttaa caaaatgtgg cagtcaaata cttgctacag 32340 tttcagtttt ggctgttaaa ggcagtttgg ctccaatatc tggaacagtt caaagtgctc 32400 atcttattat aagatttgac gaaaatggag tgctactaaa caattccttc ctggacccag 32460 aatattggaa ctttagaaat ggagatctta ctgaaggcac agcctataca aacgctgttg 32520 gattatgcc taacctatca gcttatccaa aatctcacgg taaaactgcc aaaagtaaca 32580 ttgtcagtca agtttaactta aacggagaca aaactaaacc tgtaacacta accattacac 32640 taaacggtac acaggaaaca ggagacacaa ctccaagtgc atactctatg tcattttcat 32700 gggactggtc tggccacaac tacattaatg aaatatttgc cacatcctct tacacttttt 32760 catacattgc ccaagaataa agaatcgttt gtgttatgtt tcaacgtgtt tatttttcaa 32820 ttgcagaaaa tttcaagtca tttttcattc agtagtatag ccccaccacc acatagctta 32880 tacagatcac cgtaccttaa tcaaactcac agaaccctag tattcaacct gccacctccc 32940 tcccaacaca cagagtacac agtcctttct ccccggctgg ccttaaaaag catcatatca 33000 tgggtaacag acatattctt aggtgttata ttccacacgg tttcctgtcg agccaaacgc 33060 tcatcagtga tattaataaa ctccccgggc agctcactta agttcatgtc gctgtccagc 33120 tgctgagcca caggctgctg tccaacttgc ggttgcttaa cgggcggcga aggagaagtc 33180 cacgcctaca tgggggtaga gtcataatcg tgcatcagga tagggcggtg gtgctgcagc 33240 agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc aggaatacaa catggcagtg 33300 gtctcctcag cgatgattcg caccgcccgc agcataaggc gccttgtcct ccgggcacag 33360 cagcgcaccc tgatctcact taaatcagca cagtaactgc agcacagcac cacaatattg 33420 ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca tggcggggac cacagaaccc 33480 acgtggccat cataccacaa gcgcaggtag attaagtggc gacccctcat aaacacgctg 33540 gacataaaca ttacctcttt tggcatgttg taattcacca cctcccggta ccatataaac 33600 ctctgattaa acatggcgcc atccaccacc atcctaaacc agctggccaa aacctgcccg 33660 ccggctatac actgcaggga accgggactg gaacaatgac agtggagagc ccaggactcg 33720 taaccatgga tcatcatgct cgtcatgata tcaatgttgg cacaacacag gcacacgtgc 33780 atacacttcc tcaggattac aagctcctcc cgcgttagaa ccatatccca gggaacaacc 33840 cattcctgaa tcagcgtaaa tcccacactg cagggaagac ctcgcacgta actcacgttg 33900 tgcattgtca aagtgttaca ttcgggcagc agcggatgat cctccagtat ggtagcgcgg 33960 gtttctgtct caaaaggagg tagacgatcc ctactgtacg gagtgcgccg agacaaccga 34020 gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg acgtagtcat atttcctgaa 34080 gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc cggtctcgcc gcttagatcg 34140 ctctgtgtag tagttgtagt atatccactc tctcaaagca tccaggcgcc ccctggcttc 34200 gggttctatg taaactcctt catgcgccgc tgccctgata acatccacca ccgcagaata 34260 agccacaccc agccaaccta cacattcgtt ctgcgagtca cacacgggag gagcgggaag 34320 agctggaaga accatgtttt tttttttatt ccaaaagatt atccaaaacc tcaaaatgaa 34380 gatctatta gtgaacgcgc tcccctccgg tggcgtggtc aaactctaca gccaaagaac 34440 agataatggc atttgtaaga tgttgcacaa tggcttccaa aaggcaaacg gccctcacgt 34500 ccaagtggac gtaaaggcta aacccttcag ggtgaatctc ctctataaac attccagcac 34560 cttcaaccat gcccaaataa ttctcatctc gccaccttct caatatatct ctaagcaaat 34620 cccgaatatt aagtccggcc attgtaaaaa tctgctccag agcgccctcc accttcagcc 34680 tcaagcagcg aatcatgatt gcaaaaattc aggttcctca cagacctgta taagattcaa 34740 aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc cttcgcaggg ccagctgaac 34800 ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc ccgccaggaa ccatgacaaa 34860 agaacccaca ctgattatga cacgcatact cggagctatg ctaaccagcg tagccccgat 34920 gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg ctgctcaaaa aatcaggcaa 34980 agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca tgcagataaa ggcaggtaag 35040 ctccggaacc accacagaaa aagacaccat ttttctctca aacatgtctg cgggtttctg 35100 cataaacaca aaataaaata acaaaaaaac atttaaacat tagaagcctg tcttacaaca 35160 ggaaaaacaa cccttataag cataagacgg actacggcca tgccggcgtg accgtaaaaa 35220 aactggtcac cgtgattaaa aagcaccacc gacagctcct cggtcatgtc cggagtcata 35280 atgtaagact cggtaaacac atcaggttga ttcacatcgg tcagtgctaa aaagcgaccg 35340 aaatagcccg ggggaataca tacccgcagg cgtagagaca acattacagc ccccatagga 35400 ggtataacaa aattaatagg agagaaaaac acataaacac ctgaaaaacc ctcctgccta 35460 ggcaaaatag caccctcccg ctccagaaca acatacagcg cttccacagc ggcagccata 35520 acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc gacacggcac 35580 cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag agcgagtata tataggacta 35640 aaaaatgacg taacggttaa agtccacaaa aaacacccag aaaaccgcac gcgaacctac 35700 gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc cgttttccca 35760 cgttacgtca cttcccattt taagaaaact acaattccca acacatacaa gttactccgc 35820 cctaaaacct acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc 35880 cctcattatc atattggctt caatccaaaa taaggtatat tattgatgat g 35931 <210> 36 <211> 35918 <212> DNA <213> Homo sapiens <400> 36 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgccggatg tggtaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacgg gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccaag taatatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataattct gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agacttgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgttct attattatag tcagctgacg cgcagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tgcgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat tgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gagtctgtaa tgttggcggt 780 gcaggaaggg attgacttat tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtgccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt ttttaatttt tacagttttg tggtttaaag 1200 aattttgtat tgtgattttt taaaaggtcc tgtgtctgaa cctgagcctg agcccgagcc 1260 agaaccggaa cctgcaagac ctacccggcg tcctaaaatg gtgcctgcta tcctgagacg 1320 cccgacatca cctgtgtcta gagaatgcaa tagtagtacg gatagctgtg actccggtcc 1380 ttctaacaca cctcctgaga tacacccggt ggtcccgctg tgccccatta aaccagttgc 1440 cgtgagagtt ggtgggcgtc gccaggctgt ggaatgtatc gaggacttgc ttaacgagtc 1500 tgggcaacct ttggacttga gctgtaaacg ccccaggcca taaggtgtaa acctgtgatt 1560 gcgtgtgtgg ttaacgcctt tgtttgctga atgagttgat gtaagtttaa taaagggtga 1620 gataatgttt aacttgcatg gcgtgttaaa tggggcgggg cttaaagggt atataatgcg 1680 ccgtgggcta atcttggtta catctgacct catggaggct tgggagtgtt tggaagattt 1740 ttctgctgtg cgtaacttgc tggaacagag ctctaacagt acctcttggt tttggaggtt 1800 tctgtggggc tcctcccagg caaagttagt ctgcagaatt aaggaggatt acaagtggga 1860 atttgaagag cttttgaaat cctgtggtga gctgtttgat tctttgaatc tgggtcacca 1920 ggcgcttttc caagagaagg tcatcaagac tttggatttt tccacaccgg ggcgcgctgc 1980 ggctgctgtt gcttttttga gttttataaa ggataaatgg agcgaagaaa cccatctgag 2040 cggggggtac ctgctggatt ttctggccat gcatctgtgg agagcggtgg tgagacacaa 2100 gaatcgcctg ctactgttgt cttccgtccg cccggcaata ataccgacgg aggagcaaca 2160 gcaggaggaa gccaggcggc ggcggcggca ggagcagagc ccatggaacc cgagagccgg 2220 cctggaccct cgggaatgaa tgttgtacag gtggctgaac tgtttccaga actgagacgc 2280 attttaacca ttaacgagga tgggcagggg ctaaaggggg taaagaggga gcggggggct 2340 tctgaggcta cagaggaggc taggaatcta acttttagct taatgaccag acaccgtcct 2400 gagtgtgtta cttttcagca gattaaggat aattgcgcta atgagcttga tatgctggcg 2460 cagaagtatt ccatagagca gctgaccact tactggctgc agccagggga tgattttgag 2520 gaggctatta gggtatatgc aaaggtggca cttaggccag attgcaagta caagattagc 2580 aaacttgtaa atatcaggaa ttgttgctac atttctggga acggggccga ggtggagata 2640 gatacggagg atagggtggc ctttagatgt agcatgataa atatgtggcc gggggtgctt 2700 ggcatggacg gggtggttat tatgaatgtg aggtttactg gtcccaattt tagcggtacg 2760 gttttcctgg ccaataccaa tcttatccta cacggtgtaa gcttctatgg gtttaacaat 2820 acctgtgtgg aagcctggac cgatgtaagg gttcggggct gtgcctttta ctgctgctgg 2880 aagggggtgg tgtgtcgccc caaaagcagg gcttcaatta agaaatgcct ctttgaaagg 2940 tgtaccttgg gtatcctgtc tgagggtaac tccagggtgc gccacaatgt ggcctccgac 3000 tgtggttgct tcatgctagt gaaaagcgtg gctgtgatta agcataacat ggtgtgtggc 3060 aactgcgagg acagggcctc tcagatgctg acctgctcgg acggcaactg tcacctgctg 3120 aagaccattc acgtagccag ccactctcgc aaggcctggc cagtgtttga gcacaacata 3180 ctgacccgct gttccttgca tttgggtaac aggagggggg tgtttctacc ttaccaatgc 3240 aatttgagtc acactaagat attgcttgag cccgagagca tgtccaaggt gaacctgaac 3300 ggggtgtttg acatgaccat gaagatctgg aaggtgctga ggtacgatga gacccgcacc 3360 aggtgcagac cctgcgagtg tggcggtaaa catattagga accagcctgt gatgctggat 3420 gtgaccgagg agctgaggcc cgatcacttg gtgctggcct gcacccgcgc tgagtttggc 3480 tctagcgatg aagatacaga ttgaggtact gaaatgtgtg ggcgtggctt aagggtggga 3540 aagaatatat aaggtggggg tctcatgtag ttttgtatct gttttgcagc agccgccgcc 3600 atgagcgcca actcgtttga tggaagcatt gtgagctcat atttgacaac gcgcatgccc 3660 ccatgggccg gggtgcgtca gaatgtaatg ggctccagca ttgatggtcg ccccgtcctg 3720 cccgcaaact ctactacctt gacctacgag accgtgtctg gaacgccgtt ggagactgca 3780 gcctccgccg ccgcttcagc cgctgcagcc accgcccgcg ggattgtgac tgactttgct 3840 ttcctgagcc cgcttgcaag cagtgcagct tcccgttcat ccgcccgcga tgacaagttg 3900 acggctcttt tggcacaatt ggattctttg acccgggaac ttaatgtcgt ttctcagcag 3960 ctgttggatc tgcgccagca ggtttctgcc ctgaaggctt cctcccctcc caatgcggtt 4020 taaaacataa ataaaaacca gactctgttt ggatttggat caagcaagtg tcttgctgtc 4080 tttatttagg ggttttgcgc gcgcggtagg cccgggacca gcggtctcgg tcgttgaggg 4140 tcctgtgtat tttttccagg acgtggtaaa ggtgactctg gatgttcaga tacatgggca 4200 taagcccgtc tctggggtgg aggtagcacc actgcagagc ttcatgctgc ggggtggtgt 4260 tgtagatgat ccagtcgtag caggagcgct gggcgtggtg cctaaaaatg tctttcagta 4320 gcaagctgat tgccagggga aggcccttgg tgtaagtgtt tacaaagcgg ttaagctggg 4380 atgggtgcat acgtggggat atgagatgca tcttggactg tatttttagg ttggctatgt 4440 tcccagccat atccctccgg ggattcatgt tgtgcagaac caccagcaca gtgtatccgg 4500 tgcacttggg aaatttgtca tgtagcttag aaggaaatgc gtggaagaac ttggagacgc 4560 ccttgtgacc tccaagattt tccatgcatt cgtccataat gatggcaatg ggtccacggg 4620 cggcggcctg ggcgaagata tttctgggat cactaacgtc atagttgtgt tccaggatga 4680 gatcgtcata ggccattttt acaaagcgcg ggcggagggt gccagactgc ggtataatgg 4740 ttccatccgg cccaggggcg tagttaccct cacagatttg catttcccac gctttgagtt 4800 cagatggggg gatcatgtct acctgcgggg cgatgaagaa aaccgtttcc ggggtagggg 4860 agatcagctg ggaagaaagc aggttcctga gcagctgcga cttaccgcag ccggtgggcc 4920 cgtaaatcac acctattacc ggctgcaact ggtagttaag agagctgcag ctgccgtcat 4980 ccctgagcag gggggccact tcgttaagca tgtccctgac tcgcatgttt tccctgacca 5040 aatccgccag aaggcgctcg ccgcccagcg atagcagttc ttgcaaggaa gcaaagtttt 5100 tcaacggttt gaggccgtcc gccgtaggca tgcttttgag cgtttgacca agcagttcca 5160 ggcggtccca cagctcggtc acgtgctcta tggcatctcg atccagcata tctcctcgtt 5220 tcgcgggttg gggcggcttt cgctgtacgg cagtagtcgg tgctcgtcca aacgggccag 5280 ggtcatgtct ttccacgggc gcagggtcct cgtcagcgta gtctgggtca cggtgaaggg 5340 gtgcgctccg ggctgcgcgc tggccagggt gcgcttgagg ctggtcctgc tggtgctgaa 5400 gcgctgccgg tcttcgccct gcgcgtcggc caggtagcat ttgaccatgg tgtcatagtc 5460 cagcccctcc gcggcgtggc ccttggcgcg cagcttgccc ttggaggagg cgccgcacga 5520 ggggcagtgc agacttttaa gggcgtagag cttgggcgcg agaaataccg attccgggga 5580 gtaggcatcc gcgccgcagg ccccgcagac ggtctcgcat tccacgagcc aggtgagctc 5640 tggccgttcg gggtcaaaaa ccaggtttcc cccatgcttt ttgatgcgtt tcttacctct 5700 ggtttccatg agccggtgtc cacgctcggt gacgaaaagg ctgtccgtgt ccccgtatac 5760 agacttgaga ggcctgtcct cgagcggtgt tccgcggtcc tcctcgtata gaaactcgga 5820 ccactctgag acgaaggctc gcgtccaggc cagcacgaag gaggctaagt gggaggggta 5880 gcggtcgttg tccactaggg ggtccactcg ctccagggtg tgaagacaca tgtcgccctc 5940 ttcggcatca aggaaggtga ttggtttata ggtgtaggcc acgtgaccgg gtgttcctga 6000 aggggggcta taaaaggggg tgggggcgcg ttcgtcctca ctctcttccg catcgctgtc 6060 tgcgagggcc agctgttggg gtgagtactc cctctcaaaa gcgggcatga cttctgcgct 6120 aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg cggtgatgcc 6180 tttgagggtg gccgcgtcca tctggtcaga aaagacaatc tttttgttgt caagcttggt 6240 ggcaaacgac ccgtagaggg cgttggacag caacttggcg atggagcgca gggtttggtt 6300 tttgtcgcga tcggcgcgct ccttggccgc gatgtttagc tgcacgtatt cgcgcgcaac 6360 gcaccgccat tcgggaaaga cggtggtgcg ctcgtcgggc accaggtgca cgcgccaacc 6420 gcggttgtgc agggtgacaa ggtcaacgct ggtggctacc tctccgcgta ggcgctcgtt 6480 ggtccagcag aggcggccgc ccttgcgcga acagaatggc ggtagtgggt ctagctgcgt 6540 ctcgtccggg gggtctgcgt ccacggtaaa gaccccgggc agcaggcgcg cgtcgaagta 6600 gtctatcttg catccttgca agtctagcgc ctgctgccat gcgcgggcgg caagcgcgcg 6660 ctcgtatggg ttgagtgggg gaccccatgg catggggtgg gtgagcgcgg aggcgtacat 6720 gccgcaaatg tcgtaaacgt agaggggctc tctgagtatt ccaagatatg tagggtagca 6780 tcttccaccg cggatgctgg cgcgcacgta atcgtatagt tcgtgcgagg gagcgaggag 6840 gtcgggaccg aggttgctac gggcgggctg ctctgctcgg aagactatct gcctgaagat 6900 ggcatgtgag ttggatgata tggttggacg ctggaagacg ttgaagctgg cgtctgtgag 6960 acctaccgcg tcacgcacga aggaggcgta ggagtcgcgc agcttgttga ccagctcggc 7020 ggtgacctgc acgtctaggg cgcagtagtc cagggtttcc ttgatgatgt catacttatc 7080 ctgtcccttt tttttccaca gctcgcggtt gaggacaaac tcttcgcggt ctttccagta 7140 ctcttggatc ggaaacccgt cggcctccga acggtaagag cctagcatgt agaactggtt 7200 gacggcctgg taggcgcagc atcccttttc tacgggtagc gcgtatgcct gcgcggcctt 7260 ccggagcgag gtgtgggtga gcgcaaaggt gtccctaacc atgactttga ggtactggta 7320 tttgaagtca gtgtcgtcgc atccgccctg ctcccagagc aaaaagtccg tgcgcttttt 7380 ggaacgcggg tttggcaggg cgaaggtgac atcgttgaaa agtatctttc ccgcgcgagg 7440 cataaagttg cgtgtgatgc ggaagggtcc cggcacctcg gaacggttgt taattacctg 7500 ggcggcgagc acgatctcgt cgaagccgtt gatgttgtgg cccacgatgt aaagttccaa 7560 gaagcgcggg gtgcccttga tggagggcaa ttttttaagt tcctcgtagg tgagctcctc 7620 aggggagctg agcccgtgtt ctgacagggc ccagtctgca agatgagggt tggaagcgac 7680 gaatgagctc cacaggtcac gggccattag catttgcagg tggtcgcgaa aggtcctaaa 7740 ctggcgacct atggccattt tttctggggt gatgcagtag aaggtaagcg ggtcttgttc 7800 ccagcggtcc catccaaggt ccacggctag gtctcgcgcg gcggtcacca gaggctcatc 7860 tccgccgaac ttcataacca gcatgaaggg cacgagctgc ttcccaaagg cccccatcca 7920 agtataggtc tctacatcgt aggtgacaaa gagacgctcg gtgcgaggat gcgagccgat 7980 cgggaagaac tggatctccc gccaccagtt ggaggagtgg ctgttgatgt ggtgaaagta 8040 gaagtccctg cgacgggccg aacactcgtg ctggcttttg taaaaacgtg cgcagtactg 8100 gcagcggtgc acgggctgta catcctgcac gaggttgacc tgacgaccgc gcacaaggaa 8160 gcagagtggg aatttgagcc cctcgcctgg cgggtttggc tggtggtctt ctacttcggc 8220 tgcttgtcct tgaccgtctg gctgctcgag gggagttatg gtggatcgga ccaccacgcc 8280 gcgcgagccc aaagtccaga tgtccgcgcg cggcggtcgg agcttgatga caacattgcg 8340 cagatgggag ctgtccatgg tctggagctc ccgcggcgac aggtcaggcg ggagctcctg 8400 caggtttacc tcgcatagcc gggtcagggc gcgggctagg tccaggtgat acctgatttc 8460 caggggctgg ttggtggcgg cgtcgatggc ttgcaagagg ccgcatcccc gcggcgcgac 8520 tacggtaccg cgcggcgggc ggtgggccgc gggggtgtcc ttggatgatg catctaaaag 8580 cggtgacgcg ggcgggcccc cggaggtagg gggggctcgg gacccgccgg gagagggggc 8640 aggggcacgt cggcgccgcg cgcgggcagg agctggtgct gcgcgcggag gttgctggcg 8700 aacgcgacga cgcggcggtt gatctcctga atctggcgcc tctgcgtgaa gacgacgggc 8760 ccggtgagct tgaacctgaa agagagttcg acagaatcaa tttcggtgtc gttgacggcg 8820 gcctggcgca aaatctcctg cacgtctcct gagttgtctt gataggcgat ttcggccatg 8880 aactgctcga tctcttcctc ctggagatct ccgcgtccgg ctcgctccac ggtggcggcg 8940 aggtcgttgg agatgcgggc catgagctgc gagaaggcgt tgaggcctcc ctcgttccag 9000 acgcggctgt agaccacgcc cccttcggca tcgcgggcgc gcatgaccac ctgcgcgaga 9060 ttgagctcca cgtgccgggc gaagacggcg tagtttcgca ggcgctgaaa gaggtagttg 9120 agggtggtgg cggtgtgttc tgccacgaag aagtacataa cccagcgtcg caacgtggat 9180 tcgttgatat cccccaaggc ctcaaggcgc tccatggcct cgtagaagtc cacggcgaag 9240 ttgaaaaact gggagttgcg cgccgacacg gttaactcct cctccagaag acggatgagc 9300 tcggcgacag tgtcgcgcac ctcgcgctca aaggctacag gggcctcttc ttcttcaatc 9360 tcctcttcca taagggcctc cccttcttct tcttctggcg gcggtggggg aggggggaca 9420 cggcggcgac gacggcgcac cgggaggcgg tcgacaaagc gctcgatcat ctccccgcgg 9480 cgacggcgca tggtctcggt gacggcgcgg ccgttctcgc gggggcgcag ttggaagacg 9540 ccgcccgtca tgtcccggtt atgggttggc ggggggctgc cgtgcggcag ggatacggcg 9600 ctaacgatgc atctcaacaa ttgttgtgta ggtactccgc caccgaggga cctgagcgag 9660 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa 9720 ggtaggctga gcaccgtggc gggcggcagc gggcggcggt cggggttgtt tctggcggag 9780 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgacagaagc 9840 accatgtcct tgggtccggc ctgctgaatg cgcaggcggt cggccatgcc ccaggcttcg 9900 ttttgacatc ggcgcaggtc tttgtagtag tcttgcatga gcctttctac cggcacttct 9960 tcttctcctt cctcttgtcc tgcatctctt gcatctatcg ctgcggcggc ggcggagttt 10020 ggccgtaggt ggcgccctct tcctcccatg cgtgtgaccc cgaagcccct catcggctga 10080 agcagggcca ggtcggcgac aacgcgctcg gctaatatgg cctgctgcac ctgcgtgagg 10140 gtagactgga agtcatccat gtccacaaag cggtggtatg cgcccgtgtt gatggtgtaa 10200 gtgcagttgg ccataacgga ccagttaacg gtctggtgac ccggctgcga gagctcggtg 10260 tacctgagac gcgagtaagc ccttgagtca aagacgtagt cgttgcaagt ccgcaccagg 10320 tactgatatc ccaccaaaaa gtgcggcggc ggctggcggt agaggggcca gcgtagggtg 10380 gccggggctc cgggggcgag gtcttccaac ataaggcgat gatatccgta gatgtacctg 10440 gacatccagg tgatgccggc ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc 10500 cagatgttgc gcagcggcaa aaagtgctcc atggtcggga cgctctggcc ggtgaggcgt 10560 gcgcagtcgt tgacgctcta gaccgtgcaa aaggagagcc tgtaagcggg cactcttccg 10620 tggtctggtg gataaattcg caagggtatc atggcggacg accggggttc gaaccccgga 10680 tccggccgtc cgccgtgatc catgcggtta ccgcccgcgt gtcgaaccca ggtgtgcgac 10740 gtcagacaac gggagagcgc tccttttggc ttccttccag gcgcggcggc tgctgcgcta 10800 gcttttttgg ccactggccg cgcgcggcgt aagcggttag gctggaaagc gaaagcatta 10860 agtggctcgc tccctgtagc cggagggtta ttttccaagg gttgagtcgc aggacccccg 10920 gttcgagtct cgggccggcc ggactgcggc gaacgggggt ttgcctcccc gtcatgcaag 10980 accccgcttg caaattcctc cggaaacagg gacgagcccc ttttttgctt ttcccagatg 11040 catccggtgc tgcggcagat gcgtccccct cctcagcagc ggcaagagca agagcagcgg 11100 cagacatgca gggcaccctc cccttctcct accgcgtcag gaggggcaac atccgcggct 11160 gacgcggcgg cagatggtga ttacgaaccc ccgcggcgcc gggcccggca ctacctggac 11220 ttggaggagg gcgagggcct ggcgcggcta ggagcgccct ctcctgagcg acacccaagg 11280 gtgcagctga agcgtgacac gcgcgaggcg tacgtgccgc ggcagaacct gtttcgcgac 11340 cgcgagggag aggagcccga ggagatgcgg gatcgaaatt tccacgcagg gcgcgagttg 11400 cggcatggcc tgaaccgcga gcggttgctg cgcgaggagg actttgagcc cgacgcgcgg 11460 accgggatta gtcccgcgcg cgcacacgtg gcagccgccg acctggtaac cgcgtacgag 11520 cagacggtga accaggagat taactttcaa aaaagcttta acaaccacgt gcgcacgctt 11580 gtggcgcgcg aggaggtggc tataggactg atgcatctgt gggactttgt aagcgcgttg 11640 gagcaaaacc caaatagcaa gccgctcatg gcgcagctgt tccttatagt gcagcacagc 11700 agggacaacg aggcattcag ggatgcgctg ctaaacatag tagagcccga gggccgctgg 11760 ctgctcgatt tgataaacat cctgcagagc atagtggtgc aggagcgcag cttgagcctg 11820 gctgacaagg tggccgccat caactattcc atgcttagcc tgggcaagtt ttacgcccgc 11880 aagatatacc atacccctta cgttcccata gacaaggagg taaagatcga ggggttctac 11940 atgcgcatgg cgctgaaggt gcttaccttg agcgacgacc tgggcgttta tcgcaacgag 12000 cgcatccaca aggccgtaag cgtgagccgg cggcgcgagc tgagcgaccg cgagctgatg 12060 cacagcctgc aaagggccct ggctggcacg ggcagcggcg atagagaggc cgagtcctac 12120 tttgacgcgg gcgctgacct gcgctgggcc ccaagccgac gcgccctgga ggcagctggg 12180 gccggacctg ggctggcggt ggcacccgcg cgcgctggca acgtcggcgg cgtggaggaa 12240 tatgacgagg acgatgagta cgagccagag gacggcgagt actaagcggt gatgtttctg 12300 atcagatgat gcaagacgca acggacccgg cggtgcgggc ggcgctgcag agccagccgt 12360 ccggccttaa ctccacggac gactggcgcc aggtcatgga ccgcatcatg tcgctgactg 12420 cgcgcaaccc tgacgcgttc cggcagcagc cgcaggccaa ccggctctcc gcaattctgg 12480 aagcggtggt cccggcgcgc gcaaacccca cgcacgagaa ggtgctggcg atcgtaaacg 12540 cgctggccga aaacagggcc atccggcccg acgaggccgg cctggtctac gacgcgctgc 12600 ttcagcgcgt ggctcgttac aacagcggca acgtgcagac caacctggac cggctggtgg 12660 gggatgtgcg cgaggccgtg gcgcagcgtg agcgcgcgca gcagcagggc aacctgggct 12720 ccatggttgc actaaacgcc ttcctgagta cacagcccgc caacgtgccg cggggacagg 12780 aggactacac caactttgtg agcgcactgc ggctaatggt aactgagaca ccgcaaagtg 12840 aggtgtacca gtccgggcca gactattttt tccagaccag tagacaaggc ctgcagaccg 12900 taaacctgag ccaggctttc aagaacttgc aggggctgtg gggggtgcgg gctcccacag 12960 gcgaccgcgc gaccgtgtct agcttgctga cgcccaactc gcgcctgttg ctgctgctaa 13020 tagcgccctt cacggacagt ggcagcgtgt cccgggacac atacctaggt cacttgctga 13080 cactgtaccg cgaggccata ggtcaggcgc atgtggacga gcatactttc caggagatta 13140 caagtgtcag ccgcgcgctg ggacaggagg acacgggcag cctggaggcg accctaaact 13200 acctgctgac caaccggcgg cagaagatcc cctcgttgca cagtttaaac agcgaggagg 13260 agcgcatctt gcgctatgtg cagcagagcg tgagccttaa cctgatgcgc gacggggtaa 13320 cgcccagcgt ggcgctggac atgaccgcgc gcaacatgga accgggcatg tatgcctcaa 13380 accggccgtt tatcaaccgc ctaatggact acttgcatcg cgcggccgcc gtgaaccccg 13440 agtatttcac caatgccatc ttgaacccgc actggctacc gccccctggt ttctacaccg 13500 ggggattcga ggtgcccgag ggtaacgatg gattcctctg ggacgacata gacgacagcg 13560 tgttttcccc gcaaccgcag accctgctag agttgcaaca gcgcgagcag gcagaggcgg 13620 cgctgcgaaa ggaaagcttc cgcaggccaa gcagcttgtc tgatctaggc gctgcagccc 13680 cgcggtcaga tgctagtagc ccatttccaa gcttgatagg gtctcttacc agcactcgca 13740 ccacccgccc gcgcctgctg ggcgaggagg agtacctaaa caactcgctg ctgcagccgc 13800 agcgcgaaaa gaacctgcct ccagcgtttc ccaacaacgg gatagagagc ctagtggaca 13860 agatgagtag atggaagacg tacgcgcagg agcacaggga tgtgcccggc ccgcgcccgc 13920 ccacccgtcg tcaaaggcac gaccgtcagc ggggtctggt gtgggaggac gatgactcgg 13980 cagacgacag cagcgtcctg gatttgggag ggagtggcaa cccgtttgcg caccttcgcc 14040 ccaggctggg gagaatgttt taaaaaaaaa aaaaaaaagc atgatgcaaa ataaaaaact 14100 caccaaggcc atggcaccga gcgttggttt tcttgtattc cccttagtat gcggcgcgcg 14160 gcgatgtatg aggaaggtcc tcctccctcc tacgagagcg tggtgagcgc ggcgccagtg 14220 gcggcggcgc tgggttctcc cttcgatgct cccctggacc cgccgttcgt gcctccgcgg 14280 tacctgcggc ctaccggggg gagaaacagc atccgttact ctgagttggc acccctattc 14340 gacaccaccc gtgtgtacct ggtggacaac aagtcaacgg atgtggcatc cctgaactac 14400 cagaacgacc acagcaactt tttgaccacg gtcattcaaa acaatgacta cagcccgggg 14460 gaggcaagca cacagaccat caatcttgac gaccggtcgc actggggcgg cgacctgaaa 14520 accatcctgc ataccaacat gccaaatgtg aacgagttca tgtttaccaa taagtttaag 14580 gcgcgggtga tggtgtcgcg ctcgcttact aaggacaatc aggtggagct gaaatacgag 14640 tgggtggagt tcacgctgcc cgagggcaac tactccgaga ccatgaccat agaccttatg 14700 aacaacgcga tcgtggagca ctacttgaaa gtgggcaggc agaacggggt tctggaaagc 14760 gacatcgggg taaagtttga cacccgcaac ttcagactgg ggtttgaccc agtcactggt 14820 cttgtcatgc ctggggtata tacaaacgaa gccttccatc cagacatcat tttgctgcca 14880 ggatgcgggg tggacttcac ccacagccgc ctgagcaact tgttgggtat ccgcaagcgg 14940 caacccttcc aggagggctt taggatcacc tacgatgacc tggagggtgg taacattccc 15000 gcactgttgg atgtggacgc ctaccaggca agcttgaaag atgacaccga acagggcggg 15060 ggtggcgcag gcgacggcaa cagtggcagc ggcgcggaag agaactccaa cgcggcagcc 15120 gcggcaatgc agccggtgga ggacatgaac gatcatgcca ttcgcggcga cacctttgcc 15180 acacgggcgg aggagaagcg cgctgaggcc gaggcagcgg ccgaagctgc cgcccccgct 15240 gcgcaacccg aggtcgaaaa gcctcagaag aaaccggtga ttaaacccct gacagaggac 15300 agcaagaaac gcagttacaa cctaataagt aatgacagca ccttcaccca gtaccgcagc 15360 tggtaccttg catacaacta cggcgaccct cagaccggga tccgctcatg gaccctgctt 15420 tgcactcctg acgtaacctg cggctcggag caggtatact ggtcgttgcc cgacatgatg 15480 caagaccccg tgaccttccg ctccacgcgc cagatcagca actttccggt ggtgggcgcc 15540 gagctgttgc ccgtgcactc caagagcttc tacaacgacc aggccgtcta ctcccagctc 15600 atccgccagt ttacctctct gacccacgtg ttcaatcgct ttcccgagaa ccagattttg 15660 gcgcgcccgc cagcccccac catcaccacc gtcagtgaaa acgttcctgc tctcacagat 15720 cacgggacgc taccgctgcg caacagcatc ggaggagtcc agcgagtgac cattactgac 15780 gccagacgcc gcacctgccc ctacgtttac aaggccctgg gcatagtctc gccgcgcgtc 15840 ctatcgagcc gcactttttg agcaagcatg tccatcctta tatcgcccag caataacaca 15900 ggctggggcc tgcgcttccc aagcaagatg tttggcgggg ccaagaagcg ctccgaccaa 15960 cacccagtgc gcgtgcgcgg gcactaccgc gcgccctggg gcgcgcacaa acgcggccgc 16020 actgggcgca ccaccgtcga tgacgccatc gacgcggtgg tggaggaggc gcgcaactac 16080 acgcccacgc cgccgccagt gtccaccgtg gacgcggcca ttcagaccgt ggtgcgcgga 16140 gcccggcgct atgctaaaat gaagagacgg cggaggcgcg tagcacgtcg ccaccgccgc 16200 cgacccggca ctgccgccca acgcgcggcg gcggccctgc ttaaccgcgc acgtcgcacc 16260 ggccgacggg cggccatgcg ggccgctcga aggctggccg cgggtattgt cactgtgccc 16320 cccaggtcca ggcgacgagc ggccgccgca gcagccgcag ccattagtgc tatgactcag 16380 ggtcgcaggg gcaacgtgta ctgggtgcgc gactcggtta gcggcctgcg cgtgcccgtg 16440 cgcacccgcc ccccgcgcaa ctagattgca agaaaaaact acttagactc gtactgttgt 16500 atgtatccag cggcggcggc gcgcaacgaa gctatgtcca agcgcaaaat caaagaagag 16560 atgctccagg tcatcgcgcc ggagatctat ggccccccga agaaggaaga gcaggattac 16620 aagccccgaa agctaaagcg ggtcaaaaag aaaaagaaag atgatgatga tgaacttgac 16680 gacgaggtgg aactgttgca cgcgaccgcg cccaggcggc gggtacagtg gaaaggtcga 16740 cgcgtaagac gtgttttgcg acccggcacc accgtagtct tacgcccgg tgagcgctcc 16800 acccgcacct acaagcgcgt gtatgatgag gtgtacggcg acgaggacct gcttgagcag 16860 gccaacgagc gcctcgggga gtttgcctac ggaaagcggc ataaggacat gctggcgttg 16920 ccgctggacg agggcaaccc aacacctagc ctaaagccag tgacactgca gcaggtgctg 16980 cccgcgcttg caccgtccga agaaaagcgc ggcctaaagc gcgagtctgg tgacttggcg 17040 cccaccgtgc agctcatggt gcccaagcgc cagcgactgg aagatgtctt ggaaaaaatg 17100 accgtggagc ctgggctgga gcccgaggtc cgcgtgcggc caatcaagca ggtggcgccg 17160 ggactgggcg tgcagaccgt ggacgttcag atacccacca ccagtagcac cagtattgcc 17220 accgccacag agggcatgga gacacaaacg tccccggttg cctcggcgat ggcagatgcc 17280 gcggtgcagg cggccgcggc cgcgtccaaa acctctacgg aggtgcaaac ggacccgtgg 17340 atgtttcgcg tttcagcccc ccggcgtccg cgccgttcga ggaagtacgg cgccgccagc 17400 gcgctactcc ccgaatatgc cctacatcct tccatcgcgc ctacccccgg ctatcgtggc 17460 tacacctacc gccccagaag acgagcaact acccgacgcc gaaccaccac tggaacccgc 17520 cgccgccgtc gccgtcgcca gcccgtgctg gccccgattt ccgtgcgcag ggtggctcgc 17580 gaaggaggca ggaccctggt gctgccaaca gcgcgctacc accccagcat cgtttaaaag 17640 ccggtctttg tggttcttgc agatatggcc ctcacctgcc gcctccgttt cccggtgccg 17700 ggattccgag gaagaatgca ccgtaggagg ggcatggccg gccacggcct gacgggcggc 17760 atgcgtcgtg cgcaccaccg gcggcggcgc gcgtcgcacc gtcgcatgcg cggcggtatc 17820 ctgcccctcc ttattccact gatcgccgcg gcgattggcg ccgtgcccgg aattgcatcc 17880 gtggccttgc aggcgcagag acactgatta aaagcaagtt gcatgtggaa aaatcaaaat 17940 aaaaagtctg gactctcacg ctcgcttggt cctgtaacta ttttgtagaa tggaagacat 18000 caactttgcg tctctggccc cgcgacacgg ctcgcgcccg ttcatgggaa actggcaaga 18060 tatcggcacc agcaatatga gcggtggcgc cttcagctgg ggctcgctgt ggagcggcat 18120 taaaaatttc ggttccaccg ttaagaacta tggcagcaag gcctggaaca gcagcacagg 18180 ccagatgctg agggacaagt tgaaagagca aaatttccaa caaaaggtgg tagatggcct 18240 ggcctctggc attagcgggg tggtggacct ggccaaccag gcagtgcaaa ataagattaa 18300 cagtaagctt gatccccgcc ctcccgtaga ggagcctcca ccggccgtgg agacagtgtc 18360 tccagagggg cgtggcgaaa agcgtccgcg ccccgacagg gaagaaactc tggtgacgca 18420 aatagacgag cctccctcgt acgaggaggc actaaagcaa ggcttgccca ccacccgtcc 18480 catcgcgccc atggctaccg gagtgctggg ccaccacaca cccgtaacgc tggacctgcc 18540 tccccccgcc gacacccagc agaaacctgt gctgccaggc ccgtccgccg ttgttgtaac 18600 ccgtcctagc cgcgcgtccc tgcgccgcgc cgccggcggt ccgcgatcgt tgcggcccgt 18660 agccagtggc aactggcaaa gcacactgaa cagcatcgtg ggtctggggg tgcaatccct 18720 gaagcgccga cgatgcttct gatagctaac gtgtcgtatg tgtgtcatgt atgcgtccat 18780 gtcgccgcca gaggagctgc tgagccgccg cgcgcccgct ttccaagatg gctacccctt 18840 cgatgatgcc gcagtggtct tacatgcaca tctcgggcca ggacgcctcg gagtacctaa 18900 gccccgggct ggtgcagttc gcccgcgcca ccgagacgta cttcagcctg aataacaagt 18960 ttagaaaccc cacggtggcg cctacgcacg acgtgaccac agaccggtct cagcgtttga 19020 cgctgcggtt catccccgtg gaccgcgagg ataccgcata ctcgtacaag gcgcggttca 19080 ccctagctgt gggtgataac cgtgtgctag acatggcttc cacgtacttt gacatccgcg 19140 gcgtgctgga caggggccct acttttaagc cctactctgg cactgcctac aacgccctgg 19200 cccccaaggg tgccccaaat ccttgcgaat gggatgaagc tgctactgct cttgaaatga 19260 acctagaaga agaggacgat gacaacgaag acgaagtaga cgagcaagct gagcagcaaa 19320 aaactcacgt atttgggcag gcgccttatt ctggtataaa tattacaaag gagggtattc 19380 aaataggtaa tgatgctgga acacctaaat atgctgataa aacatttcaa cctgaacctc 19440 aaataggaga atctcagtgg aacgaaacag aaattaatca tgcagctggg agagttctaa 19500 aaaagactac cccaatgaaa ccatgttatg gttcatatgc taaacccaca aatgaaaacg 19560 gagggcaagg aattcttgta aagcaacaaa atggaaagct agaaagtcaa gttgaaatgc 1920 aatttttctc aactactgag gcagccgcag tcaatggtga taacttgact ccaaaagtgg 1980 tattgtatag cgaagacgtg gatatagaaa ctccagacac tcacatttct tacatgccaa 197440 ccattaagga aggtaattca agagaactaa tgggccaaca atctatgcca aataggccca 1800 attacattgc tttcagggac aattttattg gtcttatgta ttataacagc acgggtaaca 19860 tgggagttct ggcgggccaa gcatcgcagt tgaatgctgt tgtagatttg caagacagaa 19920 acacagagtt atcatatcag cttttgcttg attccattgg ggatagaacc aggtactttt 19980 ccatgtggaa tcaggctgtt gacagttatg atccagatgt tagaattatt gaaaatcatg 20040 gaactgaaga tgaacttcct aattactgct ttccacttgg aggcgtaatt aatacagaaa 20100 ctcttgctaa aataaaacct aaagcaggtg aggaaaacaa atgggagaaa gatgccacag 20160 aattttcaga taaaaatgaa ataagagttg gaaataattt tgccatggaa atcaatctaa 20220 atgccaacct gtggagaaat ttcctgtact ccaacatagc gctgtatttg cccgacaagc 20280 taaagtacag tccttccaac gtaaaaattt ctgataaccc aaacacctac gactacatga 20340 acaagcgagt ggtggctccc gggctagtgg actgctacat taaccttgga gcacgctggt 20400 cccttgacta tatggacaac gtcaacccat ttaaccacca ccgcaatgct ggcctgcgct 20460 accgctcaat gttgctgggc aatggtcgct atgtgccctt ccacatccag gtgcctcaga 20520 agttctttgc cattaaaaac ctccttctcc tgccgggctc atacacctac gagtggaact 20580 tcaggaagga tgttaacatg gttctgcaga gctccctagg aaatgaccta agggttgacg 20640 gagccagcat taagtttgat agcatttgcc tttacgccac cttcttcccc atggcccaca 20700 acaccgcctc cacgcttgag gccatgctta gaaacgacac caacgaccag tcctttaacg 20760 actatctctc cgccgccaac atgctctacc ctatacccgc caacgctacc aacgtgccca 20820 tatccatccc ctcccgcaac tgggcggctt tccgcggctg ggccttcacg cgccttaaga 20880 ctaaggaaac cccatcactg ggctcgggct acgaccctta ttacacctac tctggctcta 20940 taccctacct agatggaacc ttttacctca accacacctt taagaaggtg gccattacct 21000 ttgactcttc tgtcagctgg cctggcaatg accgcctgct tacccccaac gagtttgaaa 21060 ttaagcgctc agttgacggg gagggttaca acgttgccca gtgtaacatg accaaagact 21120 ggttcctggt acaaatgcta gctaactata acatggcta ccagggcttc tatatcccag 21180 agagctacaa ggaccgcatg tactccttct ttagaaactt ccagcccatg agccgtcagg 21240 tggtggatga tactaaatac aaggactacc aacaggtggg catcctacac caacacaaca 21300 actctggatt tgttggctac cttgccccca ccatgcgcga aggacaggcc taccctgcta 21360 acttccccta tccgcttata ggcaagaccg cagttgacag cattacccag aaaaagtttc 21420 tttgcgatcg caccctttgg cgcatcccat tctccagtaa ctttatgtcc atgggcgcac 21480 tcacagacct gggccaaaac cttctctacg ccaactccgc ccacgcgcta gcatgactt 21540 ttgaggtgga tcccatggac gagcccaccc ttctttatgt tttgtttgaa gtctttgacg 21600 tggtccgtgt gcaccagccg caccgcggcg tcatcgaaac cgtgtacctg cgcacgccct 21660 tctcggccgg caacgccaca acataaagaa gcaagcaaca tcaacaacag ctgccgccat 21720 gggctccagt gagcaggaac tgaaagccat tgtcaaagat cttggttgtg ggccatattt 21780 tttgggcacc tatgacaagc gctttccagg ctttgtttct ccacacaagc tcgcctgcgc 21840 catagtcaat acggccggtc gcgagactgg gggcgtacac tggatggcct ttgcctggaa 21900 cccgcactca aaaacatgct acctctttga gccctttggc ttttctgacc agcgactcaa 21960 gcaggtttac cagtttgagt acgagtcact cctgcgccgt agcgccattg cttcttcccc 22020 cgaccgctgt ataacgctgg aaaagtccac ccaaagcgta caggggccca actcggccgc 22080 ctgtggacta ttctgctgca tgtttctcca cgcctttgcc aactggcccc aaactcccat 22140 ggatcacaac cccaccatga accttattac cggggtaccc aattccatgc tcaacagtcc 22200 ccaggtacag cccaccctgc gtcgcaacca ggaacagctc tacagcttcc tggagcgcca 22260 ctcgccctac ttccgcagcc acagtgcgca gattaggagc gccacttctt tttgtcactt 22320 gaaaaacatg taaaaataat gtactagaga cactttcaat aaaggcaaat gcttttattt 22380 gtacactctc gggtgattat ttaccccccac ccttgccgtc tgcgccgttt aaaaatcaaa 22440 ggggttctgc cgcgcatcgc tatgcgccac tggcagggac acgttgcgat actggtgttt 22500 agtgctccac ttaaactcag gcacaaccat ccgcggcagc tcggtgaagt tttcactcca 22560 caggctgcgc accatcacca acgcgtttag caggtcgggc gccgatatct tgaagtcgca 22620 gttggggcct ccgccctgcg cgcgcgagtt gcgatacaca gggttgcagc actggaacac 22680 tatcagcgcc gggtggtgca cgctggccag cacgctcttg tcggagatca gatccgcgtc 22740 caggtcctcc gcgttgctca gggcgaacgg agtcaacttt ggtagctgcc ttcccaaaaa 22800 gggcgcgtgc ccaggctttg agttgcactc gcaccgtagt ggcatcaaaa ggtgaccgtg 22860 cccggtctgg gcgttaggat acagcgcctg cataaaagcc ttgatctgct taaaagccac 22920 ctgagccttt gcgccttcag agaagaacat gccgcaagac ttgccggaaa actgattggc 22980 cggacaggcc gcgtcgtgca cgcagcacct tgcgtcggtg ttggagatct gcaccacatt 23040 tcggccccac cggttcttca cgatcttggc cttgctagac tgctccttca gcgcgcgctg 23100 cccgttttcg ctcgtcacat ccatttcaat cacgtgctcc ttatttatca taatgcttcc 23160 gtgtagacac ttaagctcgc cttcgatctc agcgcagcgg tgcagccaca acgcgcagcc 23220 cgtgggctcg tgatgcttgt aggtcacctc tgcaaacgac tgcaggtacg cctgcaggaa 23280 tcgccccatc atcgtcacaa aggtcttgtt gctggtgaag gtcagctgca acccgcggtg 23340 ctcctcgttc agccaggtct tgcatacggc cgccagagct tccacttggt caggcagtag 23400 tttgaagttt gcctttagat cgttatccac gtggtacttg tccatcagcg cgcgcgcagc 23460 ctccatgccc ttctcccacg cagacacgat cggcacactc agcgggttca tcaccgtaat 23520 ttcactttcc gcttcgctgg gctcttcctc ttcctcttgc gtccgcatac cacgcgccac 23580 tgggtcgtct tcattcagcc gccgcactgt gcgcttacct cctttgccat gcttgattag 23640 caccggtggg ttgctgaaac ccaccatttg tagcgccaca tcttctcttt cttcctcgct 23700 gtccacgatc acctctggtg atggcgggcg ctcgggcttg ggagaagggc gcttcttttt 23760 cttcttgggc gcaatggcca aatccgccgc cgaggtcgat ggccgcgggc tgggtgtgcg 23820 cggcaccagc gcgtcttgtg atgagtcttc ctcgtcctcg gactcgatac gccgcctcat 23880 ccgctttttt gggggcgccc ggggaggcgg cggcgacggg gacggggacg acacgtcctc 23940 catggttggg ggacgtcgcg ccgcaccgcg tccgcgctcg ggggtggttt cgcgctgctc 24000 ctcttcccga ctggccattt ccttctccta taggcagaaa aagatcatgg agtcagtcga 24060 gaagaaggac agcctaaccg ccccctctga gttcgccacc accgcctcca ccgatgccgc 24120 caacgcgcct accaccttcc ccgtcgaggc acccccgctt gaggaggagg aagtgattat 24180 cgagcaggac ccaggttttg taagcgaaga cgacgaggac cgctcagtac caacagagga 24240 taaaaagcaa gaccaggaca acgcagaggc aaacgaggaa caagtcgggc ggggggacga 24300 aaggcatggc gactacctag atgtgggaga cgacgtgctg ttgaagcatc tgcagcgcca 24360 gtgcgccatt atctgcgacg cgttgcaaga gcgcagcgat gtgcccctcg ccatagcgga 24420 tgtcagcctt gcctacgaac gccacctatt ctcaccgcgc gtacccccca aacgccaaga 24480 aaacggcaca tgcgagccca acccgcgcct caacttctac cccgtatttg ccgtgccaga 24540 ggtgcttgcc acctatcaca tctttttcca aaactgcaag atacccctat cctgccgtgc 24600 caaccgcagc cgagcggaca agcagctggc cttgcggcag ggcgctgtca tacctgatat 24660 cgcctcgctc gacgaagtgc caaaaatctt tgagggtctt ggacgcgacg agaagcgcgc 24720 ggcaaacgct ctgcaacaag aaaacagcga aaatgaaagt cactgtggag tgctggtgga 24780 actcgagggt gacaacgcgc gcctagccgt gctaaaacgc agcatcgagg tcacccactt 24840 tgcctacccg gcacttaacc taccccccaa ggttatgagc acagtcatga gtgagctgat 24900 cgtgcgccgt gcacagcccc tggagaggga tgcaaatttg caagaacaaa cagaggaggg 24960 cctacccgca gttggcgacg agcagctagc gcgctggctt caaacgcgcg agcctgccga 25020 cttggaggag cgacgcaagc taatgatggc cgcagtgctc gttaccgtgg agcttgagtg 25080 catgcagcgg ttctttgctg acccggagat gcagcgcaag ctagaggaaa cattgcacta 25140 cacctttcga cagggctacg tacgccaggc ctgcaagatc tccaacgtgg agctctgcaa 25200 cctggtctcc taccttggaa ttttgcacga aaaccgcctt gggcaaaacg tgcttcattc 25260 cacgctcaag ggcgaggcgc gccgcgacta cgtccgtgac tgcgtttact tatttctatg 25320 ctacacctgg cagacggcca tgggcgtttg gcagcagtgc ttggaggagt gcaacctcaa 25380 ggagctgcag aaactgctaa agcaaaactt gaaggaccta tggacggcct tcaacgagcg 25440 ctccgtggcc gcgcacctgg cggacatcat tttccccgaa cgcctgctta aaaccctgca 25500 acagggtctg ccagacttca ccagtcaaag catgttgcag aactttagga actttatcct 25560 agagcgctca ggaatcttgc ccgccacctg ctgtgcactt cctagcgact ttgtgcccat 25620 taagtaccgc gaatgccctc cgccgctttg gggccactgc taccttctgc agctagccaa 25680 ctaccttgcc taccactctg acataatgga agacgtgagc ggtgacggtc tactggagtg 25740 tcactgtcgc tgcaacctat gcaccccgca ccgctccctg gtttgcaatt cgcagctgct 25800 taacgaaagt caaattatcg gtacctttga gctgcagggt ccctcgcctg acgaaaagtc 25860 cgcggctccg gggttgaaac tcactccggg gctgtggacg tcggcttacc ttcgcaaatt 25920 tgtacctgag gactaccacg cccacgagat taggttctac gaagaccaat cccgcccgcc 25980 taatgcggag cttaccgcct gcgtcattac ccagggccac attcttggcc aattgcaagc 26040 catcaacaaa gcccgccaag agtttctgct acgaaaggga cggggggttt acttggaccc 26100 ccagtccggc gaggagctca acccaatccc cccgccgccg cagccctatc agcagcagcc 26160 gcgggccctt gcttcccagg atggcaccca aaaagaagct gcagctgccg ccgccaccca 26220 cggacgagga ggaatactgg gacagtcagg cagaggaggt tttggacgag gaggaggagg 26280 acatgatgga agactgggag agcctagacg aggaagcttc cgaggtcgaa gaggtgtcag 26340 acgaaacacc gtcaccctcg gtcgcattcc cctcgccggc gccccagaaa tcggcaaccg 26400 gttccagcat ggctacaacc tccgctcctc aggcgccgcc ggcactgccc gttcgccgac 26460 ccaaccgtag atgggacacc actggaacca gggccggtaa gtccaagcag ccgccgccgt 26520 tagcccaaga gcaacaacag cgccaaggct accgctcatg gcgcgggcac aagaacgcca 26580 tagttgcttg cttgcaagac tgtgggggca acatctcatt cgcccgccgc tttcttctct 26640 accatcacgg cgtggccttc ccccgtaaca tcctgcatta ctaccgtcat ctctacagcc 26700 cctactgcac cggcggcagc ggcagcaaca gcagcggcca cacagaagca aaggcgaccg 26760 gatagcaaga ctctgacaaa gcccaagaaa tccacagcgg cggcagcagc aggaggagga 26820 gcgctgcgtc tggcgcccaa cgaacccgta tcgacccgcg agcttagaaa caagattttt 26880 cccactctgt atgctatatt tcaacagagc aggggccaag aacaagagct gaaaataaaa 26940 aacaggtctc tgcgatccct cacccgcagc tgcctgtatc acaaaagcga agatcagctt 27000 cggcgcacgc tggaagacgc ggaggctctc ttcagtaaat actgcgcgct gactcttaag 27060 gactagtttc gcgccctttc tcaaatttaa gcgcgaaaac tacgtcatct ccagcggcca 27120 cacccggcgc cagcacctgt tgtcagcgcc attatgagca aggaaattcc cacgccctac 27180 atgtggagtt accagccaca aatgggactt gcggctggag ctgcccaaga ctactcaacc 27240 cgaataaact acatgagcgc gggaccccac atgatatccc gggtcaacgg aatacgcgcc 27300 caccgaaacc gaattctcct ggaacaggcg gctattacca ccacacctcg taataacctt 27360 aatccccgta gttggcccgc tgccctggtg taccaggaaa gtcccgctcc caccactgtg 27420 gtacttccca gagacgccca ggccgaagtt cagatgacta actcaggggc gcagcttgcg 27480 ggcggctttc gtcacagggt gcggtcgcca ggacagggta taactcacct gacaatcaga 27540 gggcgaggta ttcagctcaa cgacgagtcg gtgagctcct cgcttggtct ccgtccggac 27600 gggacatttc agatcggcgg cgccggccgc tcttcattca cgcctcgtca ggcaatccta 27660 actctgcaga cctcgtcctc tgagccgcgc tccggaggca ttggaactct gcaatttatt 27720 gaggagtttg tgccatcggt ctactttaac cccttctcgg gacctcccgg ccactatccg 27780 gatcagttta ttcctaactt tgacgcggta aaggactcgg cggacggcta cgactgaatg 27840 ttaagtggag aggcagagca actgcgcctg aaacacctgg tccactgtcg ccgccacaag 27900 tgctttgccc gcgactccgg tgagttttgc tactttgaat tgcccgagga tcatatcgag 27960 ggcccggcgc acggcgtccg gcttaccacc cagggagagc ttgcccgtag cctgattcgg 28020 gagtttaccc agcgccccct gctagttgag cgggacaggg gaccctgtgt tctcactgtg 28080 atttgcaact gtcctaaccc tggattacat caagatcttt gttgccatct ctgtgctgag 28140 tataataaat acagaaatta aaatatactg gggctcctat cgccatcctg taaacgccac 28200 cgtcttcacg cgcccaagca aaccaaggcg aaccttacct ggtactttta acatctctcc 28260 ctctgtgatt tacaacagtt tcaatccaga cggagtgagt ctacgagaga acctctccga 28320 gctcagctac tccatcagaa aaaacaccac cctccttacc tgccgggaac gtacgagtgc 28380 gtcaccggcc gctgcaccac acctaccgcc tgaccgtaaa ccagactttt cccggacaga 28440 cctcaataac tctgtttacc agaacaggag gtgagcttag aaaaccctta gggtattagg 28500 ccaaaggcgc agctactgtg gggtttatga acaattcaag caactctacg ggctattcta 28560 attcagattt ctctagaatc ggggttgggg ttattctctg tcttgtgatt ctctttattc 28620 ttatactaac gcttctctgc ctaaggctcg ccgcctgctg tgtgcacatt tgcatttatt 28680 gtcagctttt taaacgctgg ggtcgccacc caagatgatt aggtacataa tcctaggttt 28740 actcaccctt gcgttagccc acggtaccac ccaaaaggtg gattttaagg agccagcctg 28800 taatgttaca ttcgcagctg aagctaatga gtgcaccact cttataaaat gcaccacaga 28860 acatgaaaag ctgcttattc gccacaaaaa caaaattggc aagtatgctg tttatgctat 28920 ttggcagcca ggtgacacta cagagtataa tgttacagtt ttccagggta aaagtcataa 28980 aacttttatg tatacttttc cattttatga aatgtgcgac attaccatgt acatgagcaa 29040 acagtataag ttgtggcccc cacaaaattg tgtggaaaac actggcactt tctgctgcac 29100 tgctatgcta attacagtgc tcgctttggt ctgtacccta ctctatatta aatacaaaag 29160 cagacgcagc tttattgagg aaaagaaaat gccttaattt actaagttac aaaactaatg 29220 tcaccactaa ctgctttact cgctgcttgc aaaacaaatt caaaaagtta gcattataat 29280 tagaatagga tttaaacccc ccggtcattt cctgctcaat accattcccc tgaacaattg 29340 actctatgtg ggatatgctc cagcactaca accttgaagt caggcttcct ggatgtcagc 29400 atctgacttt ggccagcacc tgtcccgcgg atttgttcca gtccaactac agcgacccac 29460 cctaacagag atgaccaaca caaccaacgc ggccgccgct accggactta catctaccac 29520 aaatacaccc caagtttctg cctttgtcaa taactgggat aacttgggca tgtggtggtt 29580 ctccatagcg cttatgtttg tatgccttat tattatgtgg cttatctgct gcctaaagcg 29640 caaacgcgcc cgaccaccca tctatagtcc catcattgtg ctacacccaa acaatgatgg 29700 aatccataga ttggacggac tgaaacacat gttcttttct cttacagtat gattaaatga 29760 gacatgattc ctcgagtttt tatattactg acccttgttg cgcttttttg tgcgtgctcc 29820 acattggctg cggtttctca catcgaagta gactgcattc cagccttcac agtctatttg 29880 ctttacggat ttgtcaccct cacgctcatc tgcagcctca tcactgtggt catcgccttt 29940 atccagtgca ttgactgggt ctgtgtgcgc tttgcatatc tcagacacca tccccagtac 30000 agggacagga ctatagctga gcttcttaga attctttaat tatgaaattt actgtgactt 30060 ttctgctgat tatttgcacc ctatctgcgt tttgttcccc gacctccaag cctcaaagac 30120 atatatcatg cagattcact cgtatatgga atattccaag ttgctacaat gaaaaaagcg 30180 atctttccga agcctggtta tatgcaatca tctctgttat ggtgttctgc agtaccatct 30240 tagccctagc tatatatccc taccttgaca ttggctggaa cgcaatagat gccatgaacc 30300 acccaacttt ccccgcgccc gctatgcttc cactgcaaca agttgttgcc ggcggctttg 30360 tcccagccaa tcagcctcgc ccaccttctc ccacccccac tgaaatcagc tactttaatc 30420 taacaggagg agatgactga caccctagat ctagaaatgg acggaattat tacagagcag 30480 cgcctgctag aaagacgcag ggcagcggcc gagcaacagc gcatgaatca agagctccaa 30540 gacatggtta acttgcacca gtgcaaaagg ggtatctttt gtctggtaaa gcaggccaaa 30600 gtcacctacg acaataatac caccggacac cgccttagct acaagttgcc aaccaagcgt 30660 cagaaattgg tggtcatggt gggagaaaag cccattacca taactcagca ctcggtagaa 30720 accgaaggct gcattcactc accttgtcaa ggacctgagg atctctgcac ccttattaag 30780 accctgtgcg gtctcaaaga tcttattccc tttaactaat aaaaaaaaaa ataataaagc 30840 atcacttact taaaatcagt tagcaaattt ctgtccagtt tattcagcag cacctccttg 30900 ccctcctccc agctctggta ttgcagcttc ctcctggctg caaactttct ccacaatcta 30960 aatggaatgt cagtttcctc ctgttcctgt ccatccgcac ccactatctt catgttgttg 31020 cagatgaagc gcgcaagacc gtctgaagat accttcaacc ccgtgtatcc atatgacacg 31080 gaaaccggtc ctccaactgt gccttttctt actcctccct ttgtatcccc caatgggttt 31140 caagagagtc cccctggggt actctctttg cgcctatccg aacctctagt tacctccaat 31200 ggcatgcttg cgcttaaaat gggcaacggc ctctctctgg acgaggccgg caaccttacc 31260 tcccaaaatg taaccactgt cagcccacct ctcaaaaaaa ccaagtcaaa cataaacctg 31320 gaaatatctg cacccctcac agttacctca gaagccctaa ctgtggctgc cgccgcacct 31380 ttaatggtcg cgggcaacac actcaccatg caatcacagg ccccgctaac cgtgcacgac 31440 tccaaactta gcattgccac ccaaggacca ctcacagtgt cagaaggaaa gctagccctg 31500 caaacatcag gccccctcac cactaccgat agcagtaccc ttactatcac tgcctcaccc 31560 cctctaacta ctgccactgg tagcttgggc attgacttga aagagcccat ttatacacaa 31620 aatggaaaac taggactaaa gtacggggct cctttgcatg taacagacga cctaaacact 31680 ttgaccgtag caactggtcc aggtgtgact attaataata cttccttgca aactaaagtt 31740 actggagcct tgggttttga ttcacaaggc aatatgcaac ttaatgtagc aggaggacta 31800 aggattgatt ctcaaaacag acgccttata cttgatgtta gttatccgtt tgatgctcaa 31860 aaccaactaa atctaagact aggacagggc cctcttttta taaactcagc ccacgacttg 31920 gatattaact acaacaaagg cctttacttg tttacagctt caaacaattc caaaaagctt 31980 gaggttaacc taagcactgc caaggggttg atgtttgacg ctacagccat agccattaat 32040 gcaggagatg ggcttgaatt tggttcacct aatgcaacaa acacaaatcc cctcaaaaca 32100 aaaattggcc atggcctaga atttgattca aacaaggcta tggttcctaa actaggaact 32160 ggccttagtt ttgacagcac aggtgccatt acagtaggaa acaaaaataa tgataagcta 32220 actttgtgga ccacaccagc tccatctcct aactgtagac taaatgcaga gaaagatgct 32280 aaactcactt tggtcttaac aaaatgtggc agtcaaatac ttgctacagt ttcagttttg 32340 gctgttaaag gcagtttggc tccaatatct ggaacagttc aaagtgctca tcttattata 32400 agatttgacg aaaatggagt gctactaagc aattccttcc tggacccaga atattggaac 32460 tttagaaatg gagatcttac tgaaggcaca gcctatacaa acgctgttgg atttatgcct 32520 aacctatcag cttatccaaa atctcacggt aaaactgcca aaagtaacat tgtcagtcaa 32580 gtttacttaa atggagacaa aactaaacct gtaacactaa ccattacact aaacggtaca 32640 caggaaacag gagacacaac tccaagtgca tactctatgt cattttcatg ggactggtct 32700 ggccacaact acattaatga aatatttgcc acatcctctt acactttttc atacattgcc 32760 caagaataaa gaatcgttgt gttatgtttc aacgtgttta tttttcaatt gcagaaaatt 32820 tcaagtcatt tttcattcag tagtatagcc ccaccaccac atagcttata ttgatcaccg 32880 taccttaatc aaactcacag aaccctagta ttcaacctgc cacctccctc ccaacacaca 32940 gagtacacag tcctttctcc ccggctggcc ttaaaaagca tcatatcatg ggtaacagac 33000 atattcttag gtgttatatt ccacacggtt tcctgtcgag ccaaacgctc atcagtgata 33060 ttaataaact ccccgggcag ctcgcttaag ttcatgtcgc tgtccagctg ctgagccaca 33120 ggctgctgtc caacttgcgg ttgctcaacg ggcggcgaag gggaagtcca cgcctacat 33180 ggggtagagt cataatcgtg catcaggata gggcggtggt gctgcagcag cgcgcgaata 33240 aactgctgcc gccgccgctc cgtcctgcag gaatacaaca tggcagtggt ctcctcagcg 33300 atgattcgca ccgcccgcag cataagacgc cttgtcctcc gggcacagca gcgcaccctg 33360 atctcactta aatcagcaca gtaactgcag cacagcacca caatattgtt caaaatccca 33420 cagtgcaagg cgctgtatcc aaagctcatg gcggggacca cagaacccac gtggccatca 33480 taccacaagc gcaggtagat taagtggcga cccctcataa acacgctgga cataaacatt 33540 acctcttttg gcatgttgta attcaccacc tcccggtacc atataaacct ctgattaaac 33600 atggcgccat ccaccaccat cctaaaccag ctggccaaaa cctgcccgcc ggctatgcac 33660 tgcaaggaac cgggactgga acaatgacag tggagagccc aggactcgta accatggatc 33720 atcatgctcg tcatgatatc aatgttggca caacacaggc acacgtgcat acacttcctc 33780 aggattacaa gctcctcccg cgtcagaacc atatcccagg gaacaaccca ttcctgaatc 33840 agcgtaaatc ccacactgca gggaagacct cgcacgtaac tcacgttgtg cattgtcaaa 33900 gtgttacatt cgggcagcag cggatgatcc tccagtatgg tagcgcgggt ctctgtctca 33960 aaaggaggta ggcgatccct actgtacgga gtgcgccgag acaaccgaga tcgtgttggt 34020 cgtagtgtca tgccaaatgg aacgccggac gtagtcatat ttcctgaagc aaaaccaggt 34080 gcgggcgtga caaacagatc tgcgtctccg gtctcgtcgc ttagctcgct ctgtgtagta 34140 gttgtagtat atccactctc tcaaagcatc caggcgcccc ctggcttcgg gttctatgta 34200 aactccttca tgcgccgctg ccctgataac atccaccacc gcagaataag ccacacccag 34260 ccaacctaca cattcgttct gcgagtcaca cacgggagga gcgggaagag ctggaagaac 34320 catgtttttt ttttttttat tccaaaagat tatccaaaac ctcaaaatga agatctatta 34380 agtgaacgcg ctcccctccg gtggcgtggt caaactctac agccaaagaa cagataatgg 34440 catttgtaag atgttgcaca atggcttcca aaaggcaaac tgccctcacg tccaagtgga 34500 cgtaaaggct aaacccttca gggtgaatct cctctataaa cattccagca ccttcaacca 34560 tgcccaaata attttcatct cgccacctta tcaatatgtc tctaagtaaa tcccgaatat 34620 taagtccggc cattgtaaaa atctgctcca gagcgccctc caccttcagc ctcaagcagc 34680 gaatcatgat tgcaaaaatt caggttcctc acagacctgt ataagattca aaagcggaac 34740 attaacaaaa ataccgcgat cccgtaggtc ccttcgcagg gccagctgaa cataatcgtg 34800 caggtctgca cggaccagcg cggccacttc cccgccagga accatgacaa aagaacccac 34860 actgattatg acacgcatac tcggagctat gctaaccagc gtagccccga tgtaagcttg 34920 ttgcatgggc ggcgatataa aatgcaaggt gctgctcaaa aaatcaggca aagcctcgcg 34980 caaaaaagca agcacatcgt agtcatgctc atgcagataa aggcaggtaa gttccggaac 35040 caccacagaa aaagacacca tttttctctc aaacatgtct gcgggttcct gcataaacac 35100 aaaataaaat aacaaaaaaa aacatttaaa cattagaagc ctgtcttaca acaggaaaaa 35160 caacccttat aagcataaga cggactacgg ccatgccggc gtgaccgtaa aaaaactggt 35220 caccgtgatt aaaaagcacc accgacagtt cctcggtcat gtccggagtc ataatgtaag 35280 actcggtaaa cacatcaggt tggttaacat cggtcagtgc taaaaagcga ccgaaatagc 35340 ccgggggaat acatacccgc aggcgtagag acaacattac agcccccata ggaggtataa 35400 caaaattaat aggagagaaa aacacataaa cacctgaaaa accctcctgc ctaggcaaaa 35460 tagcaccctc ccgctccaga acaacataca gcgcttccac agcggcagcc ataacagtca 35520 gccttaccag taaaaaaacc tattaaaaaa caccactcga cacggcacca gctcaatcag 35580 tcacagtgta aaaagggcca agtacagagc gagtatatat aggactaaaa aatgacgtaa 35640 cggttaaagt ccacaaaaaa cacccagaaa accgcacgcg aacctacgcc cagaaacgaa 35700 agccaaaaaa cccacaactt cctcaaatct tcacttccgt tttcccacga tacgtcactt 35760 cccattttaa aaaaactaca attcccaata catgcaagtt actccgccct aaaacctacg 35820 tcacccgccc cgttcccacg ccccgcgcca cgtcacaaac tccaccccct cattatcata 35880 ttggcttcaa tccaaaataa ggtatattat tgatgatg 35918 <210> 37 <211> 33476 <212> DNA <213> Artificial Sequence <220> <223> pAdEasy-1 <400> 37 ttaattaaca tgcatggatc ctacgtctcg accgatgccc ttgagagcct tcaacccagt 60 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt 120 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg 180 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc 240 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat 300 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg 360 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt 420 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct 480 cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc 540 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt 600 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc 660 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg 720 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc 780 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc 840 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat 900 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga 960 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca 1020 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga 1080 acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc 1140 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca 1200 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg 1260 aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac 1320 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac 1380 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc 1440 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 1500 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 1560 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 1620 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 1680 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 1740 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 1800 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 1860 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 1920 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 1980 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 2040 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 2100 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 2160 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 2220 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 2280 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 2340 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 2400 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 2460 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 2520 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 2580 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 2640 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 2700 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 2760 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 2820 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 2880 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 2940 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 3000 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 3060 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 3120 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 3180 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 3240 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 3300 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 3360 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 3420 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 3480 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 3540 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 3600 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 3660 tctagctacg aattcttcga cagcttcgaa actagaatcg atttcgaaac tagcttaagg 3720 gtgggaaaga atatataagg tgggggtctt atgtagtttt gtatctgttt tgcagcagcc 3780 gccgccgcca tgagcaccaa ctcgtttgat ggaagcattg tgagctcata tttgacaacg 3840 cgcatgcccc catgggccgg ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc 3900 cccgtcctgc ccgcaaactc tactaccttg acctacgaga ccgtgtctgg aacgccgttg 3960 gagactgcag cctccgccgc cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact 4020 gactttgctt tcctgagccc gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat 4080 gacaagttga cggctctttt ggcacaattg gattctttga cccgggaact taatgtcgtt 4140 tctcagcagc tgttggatct gcgccagcag gtttctgccc tgaaggcttc ctcccctccc 4200 aatgcggttt aaaacataaa taaaaaacca gactctgttt ggatttggat caagcaagtg 4260 tcttgctgtc tttatttagg ggttttgcgc gcgcggtagg cccgggacca gcggtctcgg 4320 tcgttgaggg tcctgtgtat tttttccagg acgtggtaaa ggtgactctg gatgttcaga 4380 tacatgggca taagcccgtc tctggggtgg aggtagcacc actgcagagc ttcatgctgc 4440 ggggtggtgt tgtagatgat ccagtcgtag caggagcgct gggcgtggtg cctaaaaatg 4500 tctttcagta gcaagctgat tgccaggggc aggcccttgg tgtaagtgtt tacaaagcgg 4560 ttaagctggg atgggtgcat acgtggggat atgagatgca tcttggactg tatttttagg 4620 ttggctatgt tcccagccat atccctccgg ggattcatgt tgtgcagaac caccagcaca 4680 gtgtatccgg tgcacttggg aaatttgtca tgtagcttag aaggaaatgc gtggaagaac 4740 ttggagacgc ccttgtgacc tccaagattt tccatgcatt cgtccataat gatggcaatg 4800 ggcccacggg cggcggcctg ggcgaagata tttctgggat cactaacgtc atagttgtgt 4860 tccaggatga gatcgtcata ggccattttt acaaagcgcg ggcggagggt gccagactgc 4920 ggtataatgg ttccatccgg cccaggggcg tagttaccct cacagatttg catttcccac 4980 gctttgagtt cagatggggg gatcatgtct acctgcgggg cgatgaagaa aacggtttcc 5040 ggggtagggg agatcagctg ggaagaaagc aggttcctga gcagctgcga cttaccgcag 5100 ccggtgggcc cgtaaatcac acctattacc ggctgcaact ggtagttaag agagctgcag 5160 ctgccgtcat ccctgagcag gggggccact tcgttaagca tgtccctgac tcgcatgttt 5220 tccctgacca aatccgccag aaggcgctcg ccgcccagcg atagcagttc ttgcaaggaa 5280 gcaaagtttt tcaacggttt gagaccgtcc gccgtaggca tgcttttgag cgtttgacca 5340 agcagttcca ggcggtccca cagctcggtc acctgctcta cggcatctcg atccagcata 5400 tctcctcgtt tcgcgggttg gggcggcttt cgctgtacgg cagtagtcgg tgctcgtcca 5460 gacgggccag ggtcatgtct ttccacgggc gcagggtcct cgtcagcgta gtctgggtca 5520 cggtgaaggg gtgcgctccg ggctgcgcgc tggccagggt gcgcttgagg ctggtcctgc 5580 tggtgctgaa gcgctgccgg tcttcgccct gcgcgtcggc caggtagcat ttgaccatgg 5640 tgtcatagtc cagcccctcc gcggcgtggc ccttggcgcg cagcttgccc ttggaggagg 5700 cgccgcacga ggggcagtgc agacttttga gggcgtagag cttgggcgcg agaaataccg 5760 attccgggga gtaggcatcc gcgccgcagg ccccgcagac ggtctcgcat tccacgagcc 5820 aggtgagctc tggccgttcg gggtcaaaaa ccaggtttcc cccatgcttt ttgatgcgtt 5880 tcttacctct ggtttccatg agccggtgtc cacgctcggt gacgaaaagg ctgtccgtgt 5940 ccccgtatac agacttgaga ggcctgtcct cgagcggtgt tccgcggtcc tcctcgtata 6000 gaaactcgga ccactctgag acaaaggctc gcgtccaggc cagcacgaag gaggctaagt 6060 gggaggggta gcggtcgttg tccactaggg ggtccactcg ctccagggtg tgaagacaca 6120 tgtcgccctc ttcggcatca aggaaggtga ttggtttgta ggtgtaggcc acgtgaccgg 6180 gtgttcctga aggggggcta taaaaggggg tgggggcgcg ttcgtcctca ctctcttccg 6240 catcgctgtc tgcgagggcc agctgttggg gtgagtactc cctctgaaaa gcgggcatga 6300 cttctgcgct aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg 6360 cggtgatgcc tttgagggtg gccgcatcca tctggtcaga aaagacaatc tttttgttgt 6420 caagcttggt ggcaaacgac ccgtagaggg cgttggacag caacttggcg atggagcgca 6480 gggtttggtt tttgtcgcga tcggcgcgct ccttggccgc gatgtttagc tgcacgtatt 6540 cgcgcgcaac gcaccgccat tcgggaaaga cggtggtgcg ctcgtcgggc accaggtgca 6600 cgcgccaacc gcggttgtgc agggtgacaa ggtcaacgct ggtggctacc tctccgcgta 6660 ggcgctcgtt ggtccagcag aggcggccgc ccttgcgcga gcagaatggc ggtagggggt 6720 ctagctgcgt ctcgtccggg gggtctgcgt ccacggtaaa gaccccgggc agcaggcgcg 6780 cgtcgaagta gtctatcttg catccttgca agtctagcgc ctgctgccat gcgcgggcgg 6840 caagcgcgcg ctcgtatggg ttgagtgggg gaccccatgg catggggtgg gtgagcgcgg 6900 aggcgtacat gccgcaaatg tcgtaaacgt agaggggctc tctgagtatt ccaagatatg 6960 tagggtagca tcttccaccg cggatgctgg cgcgcacgta atcgtatagt tcgtgcgagg 7020 gagcgaggag gtcgggaccg aggttgctac gggcgggctg ctctgctcgg aagactatct 7080 gcctgaagat ggcatgtgag ttggatgata tggttggacg ctggaagacg ttgaagctgg 7140 cgtctgtgag acctaccgcg tcacgcacga aggaggcgta ggagtcgcgc agcttgttga 7200 ccagctcggc ggtgacctgc acgtctaggg cgcagtagtc cagggtttcc ttgatgatgt 7260 catacttatc ctgtcccttt tttttccaca gctcgcggtt gaggacaaac tcttcgcggt 7320 ctttccagta ctcttggatc ggaaacccgt cggcctccga acggtaagag cctagcatgt 7380 agaactggtt gacggcctgg taggcgcagc atcccttttc tacgggtagc gcgtatgcct 7440 gcgcggcctt ccggagcgag gtgtgggtga gcgcaaaggt gtccctgacc atgactttga 7500 ggtactggta tttgaagtca gtgtcgtcgc atccgccctg ctcccagagc aaaaagtccg 7560 tgcgcttttt ggaacgcgga tttggcaggg cgaaggtgac atcgttgaag agtatctttc 7620 ccgcgcgagg cataaagttg cgtgtgatgc ggaagggtcc cggcacctcg gaacggttgt 7680 taattacctg ggcggcgagc acgatctcgt caaagccgtt gatgttgtgg cccacaatgt 7740 aaagttccaa gaagcgcggg atgcccttga tggaaggcaa ttttttaagt tcctcgtagg 7800 tgagctcttc aggggagctg agcccgtgct ctgaaagggc ccagtctgca agatgagggt 7860 tggaagcgac gaatgagctc cacaggtcac gggccattag catttgcagg tggtcgcgaa 7920 aggtcctaaa ctggcgacct atggccattt tttctggggt gatgcagtag aaggtaagcg 7980 ggtcttgttc ccagcggtcc catccaaggt tcgcggctag gtctcgcgcg gcagtcacta 8040 gaggctcatc tccgccgaac ttcatgacca gcatgaaggg cacgagctgc ttcccaaagg 8100 cccccatcca agtataggtc tctacatcgt aggtgacaaa gagacgctcg gtgcgaggat 8160 gcgagccgat cgggaagaac tggatctccc gccaccaatt ggaggagtgg ctattgatgt 8220 ggtgaaagta gaagtccctg cgacgggccg aacactcgtg ctggcttttg taaaaacgtg 8280 cgcagtactg gcagcggtgc acgggctgta catcctgcac gaggttgacc tgacgaccgc 8340 gcacaaggaa gcagagtggg aatttgagcc cctcgcctgg cgggtttggc tggtggtctt 8400 ctacttcggc tgcttgtcct tgaccgtctg gctgctcgag gggagttacg gtggatcgga 8460 ccaccacgcc gcgcgagccc aaagtccaga tgtccgcgcg cggcggtcgg agcttgatga 8520 caacatcgcg cagatgggag ctgtccatgg tctggagctc ccgcggcgtc aggtcaggcg 8580 ggagctcctg caggtttacc tcgcatagac gggtcagggc gcgggctaga tccaggtgat 8640 acctaatttc caggggctgg ttggtggcgg cgtcgatggc ttgcaagagg ccgcatcccc 8700 gcggcgcgac tacggtaccg cgcggcgggc ggtgggccgc gggggtgtcc ttggatgatg 8760 catctaaaag cggtgacgcg ggcgagcccc cggaggtagg gggggctccg gacccgccgg 8820 gagagggggc aggggcacgt cggcgccgcg cgcgggcagg agctggtgct gcgcgcgtag 8880 gttgctggcg aacgcgacga cgcggcggtt gatctcctga atctggcgcc tctgcgtgaa 8940 gacgacgggc ccggtgagct tgaacctgaa agagagttcg acagaatcaa tttcggtgtc 9000 gttgacggcg gcctggcgca aaatctcctg cacgtctcct gagttgtctt gataggcgat 9060 ctcggccatg aactgctcga tctcttcctc ctggagatct ccgcgtccgg ctcgctccac 9120 ggtggcggcg aggtcgttgg aaatgcgggc catgagctgc gagaaggcgt tgaggcctcc 9180 ctcgttccag acgcggctgt agaccacgcc cccttcggca tcgcgggcgc gcatgaccac 9240 ctgcgcgaga ttgagctcca cgtgccgggc gaagacggcg tagtttcgca ggcgctgaaa 9300 gaggtagttg agggtggtgg cggtgtgttc tgccacgaag aagtacataa cccagcgtcg 9360 caacgtggat tcgttgatat cccccaaggc ctcaaggcgc tccatggcct cgtagaagtc 9420 cacggcgaag ttgaaaaact gggagttgcg cgccgacacg gttaactcct cctccagaag 9480 acggatgagc tcggcgacag tgtcgcgcac ctcgcgctca aaggctacag gggcctcttc 9540 ttcttcttca atctcctctt ccataagggc ctccccttct tcttcttctg gcggcggtgg 9600 gggagggggg acacggcggc gacgacggcg caccgggagg cggtcgacaa agcgctcgat 9660 catctccccg cggcgacggc gcatggtctc ggtgacggcg cggccgttct cgcgggggcg 9720 cagttggaag acgccgcccg tcatgtcccg gttatgggtt ggcggggggc tgccatgcgg 9780 cagggatacg gcgctaacga tgcatctcaa caattgttgt gtaggtactc cgccgccgag 9840 ggacctgagc gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca 9900 gtcacagtcg caaggtaggc tgagcaccgt ggcgggcggc agcgggcggc ggtcggggtt 9960 gtttctggcg gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat 10020 ggtcgacaga agcaccatgt ccttgggtcc ggcctgctga atgcgcaggc ggtcggccat 10080 gccccaggct tcgttttgac atcggcgcag gtctttgtag tagtcttgca tgagcctttc 10140 taccggcact tcttcttctc cttcctcttg tcctgcatct cttgcatcta tcgctgcggc 10200 ggcggcggag tttggccgta ggtggcgccc tcttcctccc atgcgtgtga ccccgaagcc 10260 cctcatcggc tgaagcaggg ctaggtcggc gacaacgcgc tcggctaata tggcctgctg 10320 cacctgcgtg agggtagact ggaagtcatc catgtccaca aagcggtggt atgcgcccgt 10380 gttgatggtg taagtgcagt tggccataac ggaccagtta acggtctggt gacccggctg 10440 cgagagctcg gtgtacctga gacgcgagta agccctcgag tcaaatacgt agtcgttgca 10500 agtccgcacc aggtactggt atcccaccaa aaagtgcggc ggcggctggc ggtagagggg 10560 ccagcgtagg gtggccgggg ctccgggggc gagatcttcc aacataaggc gatgatatcc 10620 gtagatgtac ctggacatcc aggtgatgcc ggcggcggtg gtggaggcgc gcggaaagtc 10680 gcggacgcgg ttccagatgt tgcgcagcgg caaaaagtgc tccatggtcg ggacgctctg 10740 gccggtcagg cgcgcgcaat cgttgacgct ctagcgtgca aaaggagagc ctgtaagcgg 10800 gcactcttcc gtggtctggt ggataaattc gcaagggtat catggcggac gaccggggtt 10860 cgagccccgt atccggccgt ccgccgtgat ccatgcggtt accgcccgcg tgtcgaaccc 10920 aggtgtgcga cgtcagacaa cgggggagtg ctccttttgg cttccttcca ggcgcggcgg 10980 ctgctgcgct agcttttttg gccactggcc gcgcgcagcg taagcggtta ggctggaaag 11040 cgaaagcatt aagtggctcg ctccctgtag ccggagggtt attttccaag ggttgagtcg 11100 cgggaccccc ggttcgagtc tcggaccggc cggactgcgg cgaacggggg tttgcctccc 11160 cgtcatgcaa gaccccgctt gcaaattcct ccggaaacag ggacgagccc cttttttgct 11220 tttcccagat gcatccggtg ctgcggcaga tgcgcccccc tcctcagcag cggcaagagc 11280 aagagcagcg gcagacatgc agggcaccct cccctcctcc taccgcgtca ggaggggcga 11340 catccgcggt tgacgcggca gcagatggtg attacgaacc cccgcggcgc cgggcccggc 11400 actacctgga cttggaggag ggcgagggcc tggcgcggct aggagcgccc tctcctgagc 11460 ggcacccaag ggtgcagctg aagcgtgata cgcgtgaggc gtacgtgccg cggcagaacc 11520 tgtttcgcga ccgcgaggga gaggagcccg aggagatgcg ggatcgaaag ttccacgcag 11580 ggcgcgagct gcggcatggc ctgaatcgcg agcggttgct gcgcgaggag gactttgagc 11640 ccgacgcgcg aaccgggatt agtcccgcgc gcgcaacacgt ggcggccgcc gacctggtaa 11700 ccgcatacga gcagacggtg aaccaggaga ttaactttca aaaaagcttt aacaaccacg 11760 tgcgtacgct tgtggcgcgc gaggaggtgg ctataggact gatgcatctg tgggactttg 11820 taagcgcgct ggagcaaaac ccaaatagca agccgctcat ggcgcagctg ttccttatag 11880 tgcagcacag cagggacaac gaggcattca gggatgcgct gctaaacata gtagagcccg 11940 agggccgctg gctgctcgat ttgataaaca tcctgcagag catagtggtg caggagcgca 12000 gcttgagcct ggctgacaag gtggccgcca tcaactattc catgcttagc ctgggcaagt 12060 tttacgcccg caagatatac catacccctt acgttcccat agacaaggag gtaaagatcg 12120 aggggttcta catgcgcatg gcgctgaagg tgcttacctt gagcgacgac ctgggcgttt 12180 atcgcaacga gcgcatccac aaggccgtga gcgtgagccg gcggcgcgag ctcagcgacc 12240 gcgagctgat gcacagcctg caaagggccc tggctggcac gggcagcggc gatagagagg 12300 ccgagtccta ctttgacgcg ggcgctgacc tgcgctgggc cccaagccga cgcgccctgg 12360 aggcagctgg ggccggacct gggctggcgg tggcacccgc gcgcgctggc aacgtcggcg 12420 gcgtggagga atatgacgag gacgatgagt acgagccaga ggacggcgag tactaagcgg 12480 tgatgtttct gatcagatga tgcaagacgc aacggacccg gcggtgcggg cggcgctgca 12540 gagccagccg tccggcctta actccacgga cgactggcgc caggtcatgg accgcatcat 12600 gtcgctgact gcgcgcaatc ctgacgcgtt ccggcagcag ccgcaggcca accggctctc 12660 cgcaattctg gaagcggtgg tcccggcgcg cgcaaacccc acgcacgaga aggtgctggc 12720 gatcgtaaac gcgctggccg aaaacagggc catccggccc gacgaggccg gcctggtcta 12780 cgacgcgctg cttcagcgcg tggctcgtta caacagcggc aacgtgcaga ccaacctgga 12840 ccggctggtg ggggatgtgc gcgaggccgt ggcgcagcgt gagcgcgcgc agcagcaggg 12900 caacctgggc tccatggttg cactaaacgc cttcctgagt acacagcccg ccaacgtgcc 12960 gcggggacag gaggactaca ccaactttgt gagcgcactg cggctaatgg tgactgagac 13020 accgcaaagt gaggtgtacc agtctgggcc agactatttt ttccagacca gtagacaagg 13080 cctgcagacc gtaaacctga gccaggcttt caaaaacttg caggggctgt ggggggtgcg 13140 ggctcccaca ggcgaccgcg cgaccgtgtc tagcttgctg acgcccaact cgcgcctgtt 13200 gctgctgcta atagcgccct tcacggacag tggcagcgtg tcccgggaca catacctagg 13260 tcacttgctg acactgtacc gcgaggccat aggtcaggcg catgtggacg agcatacttt 13320 ccaggagatt acaagtgtca gccgcgcgct ggggcaggag gacacgggca gcctggaggc 13380 aaccctaaac tacctgctga ccaaccggcg gcagaagatc ccctcgttgc acagtttaaa 13440 cagcgaggag gagcgcattt tgcgctacgt gcagcagagc gtgagcctta acctgatgcg 13500 cgacggggta acgcccagcg tggcgctgga catgaccgcg cgcaacatgg aaccgggcat 13560 gtatgcctca aaccggccgt ttatcaaccg cctaatggac tacttgcatc gcgcggccgc 13620 cgtgaacccc gagtatttca ccaatgccat cttgaacccg cactggctac cgccccctgg 13680 tttctacacc gggggattcg aggtgcccga gggtaacgat ggattcctct gggacgacat 13740 agacgacagc gtgttttccc cgcaaccgca gaccctgcta gagttgcaac agcgcgagca 13800 ggcagaggcg gcgctgcgaa aggaaagctt ccgcaggcca agcagcttgt ccgatctagg 13860 cgctgcggcc ccgcggtcag atgctagtag cccatttcca agcttgatag ggtctcttac 13920 cagcactcgc accacccgcc cgcgcctgct gggcgaggag gagtacctaa acaactcgct 13980 gctgcagccg cagcgcgaaa aaaacctgcc tccggcattt cccaacaacg ggatagagag 14040 cctagtggac aagatgagta gatggaagac gtacgcgcag gagcacaggg acgtgccagg 14100 cccgcgcccg cccacccgtc gtcaaaggca cgaccgtcag cggggtctgg tgtgggagga 14160 cgatgactcg gcagacgaca gcagcgtcct ggatttggga gggagtggca acccgtttgc 14220 gcaccttcgc cccaggctgg ggagaatgtt ttaaaaaaaa aaaagcatga tgcaaaataa 14280 aaaactcacc aaggccatgg caccgagcgt tggttttctt gtattcccct tagtatgcgg 14340 cgcgcggcga tgtatgagga aggtcctcct ccctcctacg agagtgtggt gagcgcggcg 14400 ccagtggcgg cggcgctggg ttctcccttc gatgctcccc tggacccgcc gtttgtgcct 14460 ccgcggtacc tgcggcctac cggggggaga aacagcatcc gttactctga gttggcaccc 14520 ctattcgaca ccacccgtgt gtacctggtg gacaacaagt caacggatgt ggcatccctg 14580 aactaccaga acgaccacag caactttctg accacggtca ttcaaaacaa tgactacagc 14640 ccgggggagg caagcacaca gaccatcaat cttgacgacc ggtcgcactg gggcggcgac 14700 ctgaaaacca tcctgcatac caacatgcca aatgtgaacg agttcatgtt taccaataag 14760 tttaaggcgc gggtgatggt gtcgcgcttg cctactaagg acaatcaggt ggagctgaaa 14820 tacgagtggg tggagttcac gctgcccgag ggcaactact ccgagaccat gaccatagac 14880 cttatgaaca acgcgatcgt ggagcactac ttgaaagtgg gcagacagaa cggggttctg 14940 gaaagcgaca tcggggtaaa gtttgacacc cgcaacttca gactggggtt tgaccccgtc 15000 actggtcttg tcatgcctgg ggtatataca aacgaagcct tccatccaga catcattttg 15060 ctgccaggat gcggggtgga cttcacccac agccgcctga gcaacttgtt gggcatccgc 15120 aagcggcaac ccttccagga gggctttagg atcacctacg atgatctgga gggtggtaac 15180 attcccgcac tgttggatgt ggacgcctac caggcgagct tgaaagatga caccgaacag 15240 ggcgggggtg gcgcaggcgg cagcaacagc agtggcagcg gcgcggaaga gaactccaac 15300 gcggcagccg cggcaatgca gccggtggag gacatgaacg atcatgccat tcgcggcgac 15360 acctttgcca cacgggctga ggagaagcgc gctgaggccg aagcagcggc cgaagctgcc 15420 gccccccgctg cgcaacccga ggtcgagaag cctcagaaga aaccggtgat caaacccctg 15480 acagaggaca gcaagaaacg cagttacaac ctaataagca atgacagcac cttcacccag 15540 taccgcagct ggtaccttgc atacaactac ggcgaccctc agaccggaat ccgctcatgg 15600 accctgcttt gcactcctga cgtaacctgc ggctcggagc aggtctactg gtcgttgcca 15660 gacatgatgc aagaccccgt gaccttccgc tccacgcgcc agatcagcaa ctttccggtg 15720 gtgggcgccg agctgttgcc cgtgcactcc aagagcttct acaacgacca ggccgtctac 15780 tcccaactca tccgccagtt tacctctctg acccacgtgt tcaatcgctt tcccgagaac 15840 cagattttgg cgcgcccgcc agccccccacc atcaccaccg tcagtgaaaa cgttcctgct 15900 ctcacagatc acgggacgct accgctgcgc aacagcatcg gaggagtcca gcgagtgacc 15960 attactgacg ccagacgccg cacctgcccc tacgtttaca aggccctggg catagtctcg 16020 ccgcgcgtcc tatcgagccg cactttttga gcaagcatgt ccatccttat atcgcccagc 16080 aataacacag gctggggcct gcgcttccca agcaagatgt ttggcggggc caagaagcgc 16140 tccgaccaac acccagtgcg cgtgcgcggg cactaccgcg cgccctgggg cgcgcacaaa 16200 cgcggccgca ctgggcgcac caccgtcgat gacgccatcg acgcggtggt ggaggaggcg 16260 cgcaactaca cgccccacgcc gccaccagtg tccacagtgg acgcggccat tcagaccgtg 16320 gtgcgcggag cccggcgcta tgctaaaatg aagagacggc ggaggcgcgt agcacgtcgc 16380 caccgccgcc gacccggcac tgccgcccaa cgcgcggcgg cggccctgct taaccgcgca 16440 cgtcgcaccg gccgacgggc ggccatgcgg gccgctcgaa ggctggccgc gggtattgtc 16500 actgtgcccc ccaggtccag gcgacgagcg gccgccgcag cagccgcggc cattagtgct 16560 atgactcagg gtcgcagggg caacgtgtat tgggtgcgcg actcggttag cggcctgcgc 16620 gtgcccgtgc gcacccgccc cccgcgcaac tagattgcaa gaaaaaacta cttagactcg 16680 tactgttgta tgtatccagc ggcggcggcg cgcaacgaag ctatgtccaa gcgcaaaatc 16740 aaagaagaga tgctccaggt catcgcgccg gagatctatg gccccccgaa gaaggaagag 16800 caggattaca agccccgaaa gctaaagcgg gtcaaaaaga aaaagaaaga tgatgatgat 16860 gaacttgacg acgaggtgga actgctgcac gctaccgcgc ccaggcgacg ggtacagtgg 16920 aaaggtcgac gcgtaaaacg tgttttgcga cccggcacca ccgtagtctt tacgcccggt 16980 gagcgctcca cccgcaccta caagcgcgtg tatgatgagg tgtacggcga cgaggacctg 17040 cttgagcagg ccaacgagcg cctcggggag tttgcctacg gaaagcggca taaggacatg 17100 ctggcgttgc cgctggacga gggcaaccca acacctagcc taaagcccgt aacactgcag 17160 caggtgctgc ccgcgcttgc accgtccgaa gaaaagcgcg gcctaaagcg cgagtctggt 17220 gacttggcac ccaccgtgca gctgatggta cccaagcgcc agcgactgga agatgtcttg 17280 gaaaaaatga ccgtggaacc tgggctggag cccgaggtcc gcgtgcggcc aatcaagcag 17340 gtggcgccgg gactgggcgt gcagaccgtg gacgttcaga tacccactac cagtagcacc 17400 agtattgcca ccgccacaga gggcatggag acacaaacgt ccccggttgc ctcagcggtg 17460 gcggatgccg cggtgcaggc ggtcgctgcg gccgcgtcca agacctctac ggaggtgcaa 17520 acggacccgt ggatgtttcg cgtttcagcc ccccggcgcc cgcgccgttc gaggaagtac 17580 ggcgccgcca gcgcgctact gcccgaatat gccctacat cttccattgc gcctaccccc 17640 ggctatcgtg gctacaccta ccgccccaga agacgagcaa ctacccgacg ccgaaccacc 17700 actggaaccc gccgccgccg tcgccgtcgc cagcccgtgc tggccccgat ttccgtgcgc 17760 agggtggctc gcgaaggagg caggaccctg gtgctgccaa cagcgcgcta ccaccccagc 17820 atcgtttaaa agccggtctt tgtggttctt gcagatatgg ccctcacctg ccgcctccgt 17880 ttccccggtgc cgggattccg aggaagaatg caccgtagga ggggcatggc cggccacggc 17940 ctgacgggcg gcatgcgtcg tgcgcaccac cggcggcggc gcgcgtcgca ccgtcgcatg 18000 cgcggcggta tcctgcccct ccttattcca ctgatcgccg cggcgattgg cgccgtgccc 18060 ggaattgcat ccgtggcctt gcaggcgcag agacactgat taaaaacaag ttgcatgtgg 18120 aaaaatcaaa ataaaaagtc tggactctca cgctcgcttg gtcctgtaac tattttgtag 18180 aatggaagac atcaactttg cgtctctggc cccgcgacac ggctcgcgcc cgttcatggg 18240 aaactggcaa gatatcggca ccagcaatat gagcggtggc gccttcagct ggggctcgct 18300 gtggagcggc attaaaaatt tcggttccac cgttaagaac tatggcagca aggcctggaa 18360 cagcagcaca ggccagatgc tgagggataa gttgaaagag caaaatttcc aacaaaaggt 18420 ggtagatggc ctggcctctg gcattagcgg ggtggtggac ctggccaacc aggcagtgca 18480 aaataagatt aacagtaagc ttgatccccg ccctcccgta gaggagcctc caccggccgt 18540 ggagacagtg tctccagagg ggcgtggcga aaagcgtccg cgccccgaca gggaagaaac 18600 tctggtgacg caaatagacg agcctccctc gtacgaggag gcactaaagc aaggcctgcc 18660 caccacccgt cccatcgcgc ccatggctac cggagtgctg ggccagcaca cacccgtaac 18720 gctggacctg cctccccccg ccgacaccca gcagaaacct gtgctgccag gcccgaccgc 18780 cgttgttgta acccgtccta gccgcgcgtc cctgcgccgc gccgccagcg gtccgcgatc 18840 gttgcggccc gtagccagtg gcaactggca aagcacactg aacagcatcg tgggtctggg 18900 ggtgcaatcc ctgaagcgcc gacgatgctt ctgatagcta acgtgtcgta tgtgtgtcat 18960 gtatgcgtcc atgtcgccgc cagaggagct gctgagccgc cgcgcgcccg ctttccaaga 19020 tggctacccc ttcgatgatg ccgcagtggt cttacatgca catctcgggc caggacgcct 19080 cggagtacct gagccccggg ctggtgcagt ttgcccgcgc caccgagacg tacttcagcc 19140 tgaataacaa gtttagaaac cccacggtgg cgcctacgca cgacgtgacc acagaccggt 19200 cccagcgttt gacgctgcgg ttcatccctg tggaccgtga ggatactgcg tactcgtaca 19260 aggcgcggtt caccctagct gtgggtgata accgtgtgct ggacatggct tccacgtact 19320 ttgacatccg cggcgtgctg gacaggggcc ctacttttaa gccctactct ggcactgcct 19380 acaacgccct ggctcccaag ggtgccccaa atccttgcga atgggatgaa gctgctactg 19440 ctcttgaaat aaacctagaa gaagaggacg atgacaacga agacgaagta gacgagcaag 19500 ctgagcagca aaaaactcac gtatttgggc aggcgcctta ttctggtata aatattacaa 19560 aggagggtat tcaaataggt gtcgaaggtc aaacacctaa atatgccgat aaaacatttc 1920 aacctgaacc tcaaatagga gaatctcagt ggtacgaaac agaaattaat catgcagctg 1980 ggagagtcct aaaaaagact accccaatga aaccatgtta cggttcatat gcaaaaccca 197440 caaatgaaaa tggagggcaa ggcattcttg taaagcaaca aaatggaaag ctagaaagtc 1800 aagtggaaat gcaatttttc tcaactactg aggcagccgc aggcaatggt gataacttga 19860 ctcctaaagt ggtattgtac agtgaagatg tagatataga aaccccagac actcatattt 19920 cttacatgcc cactattaag gaaggtaact cacgagaact aatgggccaa caatctatgc 19980 ccaacaggcc taattacatt gcttttaggg acaattttat tggtctaatg tattacaaca 20040 gcacgggtaa tatgggtgtt ctggcgggcc aagcatcgca gttgaatgct gttgtagatt 20100 tgcaagacag aaacacagag ctttcatacc agcttttgct tgattccatt ggtgatagaa 20160 ccaggtactt ttctatgtgg aatcaggctg ttgacagcta tgatccagat gttagaatta 20220 ttgaaaatca tggaactgaa gatgaacttc caaattactg ctttccactg ggaggtgtga 20280 ttaatacaga gactcttacc aaggtaaaac ctaaaacagg tcaggaaaat ggatgggaaa 20340 aagatgctac agaattttca gataaaaatg aaataagagt tggaaataat tttgccatgg 20400 aaatcaatct aaatgccaac ctgtggagaa atttcctgta ctccaacata gcgctgtatt 20460 tgcccgacaa gctaaagtac agtccttcca acgtaaaaat ttctgataac ccaaacacct 20520 acgactacat gaacaagcga gtggtggctc ccgggctagt ggactgctac attaaccttg 20580 gagcacgctg gtcccttgac tatatggaca acgtcaaccc atttaaccac caccgcaatg 20640 ctggcctgcg ctaccgctca atgttgctgg gcaatggtcg ctatgtgccc ttccacatcc 20700 aggtgcctca gaagttcttt gccattaaaa acctccttct cctgccgggc tcatacacct 20760 acgagtggaa cttcaggaag gatgttaaca tggttctgca gagctcccta ggaaatgacc 20820 taagggttga cggagccagc attaagtttg atagcatttg cctttacgcc accttcttcc 20880 ccatggccca caacaccgcc tccacgcttg aggccatgct tagaaacgac accaacgacc 20940 agtcctttaa cgactatctc tccgccgcca acatgctcta ccctataccc gccaacgcta 21000 ccaacgtgcc catatccatc ccctcccgca actgggcggc tttccgcggc tgggccttca 21060 cgcgccttaa gactaaggaa accccatcac tgggctcggg ctacgaccct tattacacct 21120 actctggctc tataccctac ctagatggaa ccttttacct caaccacacc tttaagaagg 21180 tggccattac ctttgactct tctgtcagct ggcctggcaa tgaccgcctg cttaccccca 21240 acgagtttga aattaagcgc tcagttgacg gggagggtta caacgttgcc cagtgtaaca 21300 tgaccaaaga ctggttcctg gtacaaatgc tagctaacta taacattggc taccagggct 21360 tctatatccc agagagctac aaggaccgca tgtactcctt ctttagaaac ttccagccca 21420 tgagccgtca ggtggtggat gatactaaat acaaggacta ccaacaggtg ggcatcctac 21480 accaacacaa caactctgga tttgttggct accttgcccc caccatgcgc gaaggacagg 21540 cctaccctgc taacttcccc tatccgctta taggcaagac cgcagttgac agcattaccc 21600 agaaaaagtt tctttgcgat cgcacccttt ggcgcatccc attctccagt aactttatgt 21660 ccatgggcgc actcacagac ctgggccaaa accttctcta cgccaactcc gcccacgcgc 21720 tagacatgac ttttgaggtg gatcccatgg acgagcccac ccttctttat gttttgtttg 21780 aagtctttga cgtggtccgt gtgcaccagc cgcaccgcgg cgtcatcgaa accgtgtacc 21840 tgcgcacgcc cttctcggcc ggcaacgcca caacataaag aagcaagcaa catcaacaac 21900 agctgccgcc atgggctcca gtgagcagga actgaaagcc attgtcaaag atcttggttg 21960 tgggccatat tttttgggca cctatgacaa gcgctttcca ggctttgttt ctccacacaa 22020 gctcgcctgc gccatagtca atacggccgg tcgcgagact gggggcgtac actggatggc 22080 ctttgcctgg aacccgcact caaaaacatg ctacctcttt gagccctttg gcttttctga 22140 ccagcgactc aagcaggttt accagtttga gtacgagtca ctcctgcgcc gtagcgccat 22200 tgcttcttcc cccgaccgct gtataacgct ggaaaagtcc acccaaagcg tacaggggcc 22260 caactcggcc gcctgtggac tattctgctg catgtttctc cacgcctttg ccaactggcc 22320 ccaaactccc atggatcaca accccaccat gaaccttatt accggggtac ccaactccat 22380 gctcaacagt ccccaggtac agcccaccct gcgtcgcaac caggaacagc tctacagctt 22440 cctggagcgc cactcgccct acttccgcag ccacagtgcg cagattagga gcgccacttc 22500 tttttgtcac ttgaaaaaca tgtaaaaata atgtactaga gacactttca ataaaggcaa 22560 atgcttttat ttgtacactc tcgggtgatt atttaccccc acccttgccg tctgcgccgt 22620 ttaaaaatca aaggggttct gccgcgcatc gctatgcgcc actggcaggg acacgttgcg 22680 atactggtgt ttagtgctcc acttaaactc aggcacaacc atccgcggca gctcggtgaa 22740 gttttcactc cacaggctgc gcaccatcac caacgcgttt agcaggtcgg gcgccgatat 22800 cttgaagtcg cagttggggc ctccgccctg cgcgcgcgag ttgcgataca cagggttgca 22860 gcactggaac actatcagcg ccgggtggtg cacgctggcc agcacgctct tgtcggagat 22920 cagatccgcg tccaggtcct ccgcgttgct cagggcgaac ggagtcaact ttggtagctg 22980 ccttcccaaa aagggcgcgt gcccaggctt tgagttgcac tcgcaccgta gtggcatcaa 23040 aaggtgaccg tgcccggtct gggcgttagg atacagcgcc tgcataaaag ccttgatctg 23100 cttaaaagcc acctgagcct ttgcgccttc agagaagaac atgccgcaag acttgccgga 23160 aaactgattg gccggacagg ccgcgtcgtg cacgcagcac cttgcgtcgg tgttggagat 23220 ctgcaccaca tttcggcccc accggttctt cacgatcttg gccttgctag actgctcctt 23280 cagcgcgcgc tgcccgtttt cgctcgtcac atccatttca atcacgtgct ccttatttat 23340 cataatgctt ccgtgtagac acttaagctc gccttcgatc tcagcgcagc ggtgcagcca 23400 caacgcgcag cccgtgggct cgtgatgctt gtaggtcacc tctgcaaacg actgcaggta 23460 cgcctgcagg aatcgcccca tcatcgtcac aaaggtcttg ttgctggtga aggtcagctg 23520 caacccgcgg tgctcctcgt tcagccaggt cttgcatacg gccgccagag cttccacttg 23580 gtcaggcagt agtttgaagt tcgcctttag atcgttatcc acgtggtact tgtccatcag 23640 cgcgcgcgca gcctccatgc ccttctccca cgcagacacg atcggcacac tcagcgggtt 23700 catcaccgta atttcacttt ccgcttcgct gggctcttcc tcttcctctt gcgtccgcat 23760 accacgcgcc actgggtcgt cttcattcag ccgccgcact gtgcgcttac ctcctttgcc 23820 atgcttgatt agcaccggtg ggttgctgaa acccaccatt tgtagcgcca catcttctct 23880 ttcttcctcg ctgtccacga ttacctctgg tgatggcggg cgctcgggct tgggagaagg 23940 gcgcttcttt ttcttcttgg gcgcaatggc caaatccgcc gccgaggtcg atggccgcgg 24000 gctgggtgtg cgcggcacca gcgcgtcttg tgatgagtct tcctcgtcct cggactcgat 24060 acgccgcctc atccgctttt ttgggggcgc ccggggaggc ggcggcgacg gggacgggga 24120 cgacacgtcc tccatggttg ggggacgtcg cgccgcaccg cgtccgcgct cgggggtggt 24180 ttcgcgctgc tcctcttccc gactggccat ttccttctcc tataggcaga aaaagatcat 24240 ggagtcagtc gagaagaagg acagcctaac cgccccctct gagttcgcca ccaccgcctc 24300 caccgatgcc gccaacgcgc ctaccacctt ccccgtcgag gcacccccgc ttgaggagga 24360 ggaagtgatt atcgagcagg acccaggttt tgtaagcgaa gacgacgagg accgctcagt 24420 accaacagag gataaaaagc aagaccagga caacgcagag gcaaacgagg aacaagtcgg 24480 gcggggggac gaaaggcatg gcgactacct agatgtggga gacgacgtgc tgttgaagca 24540 tctgcagcgc cagtgcgcca ttatctgcga cgcgttgcaa gagcgcagcg atgtgcccct 24600 cgccatagcg gatgtcagcc ttgcctacga acgccaccta ttctcaccgc gcgtaccccc 24660 caaacgccaa gaaaacggca catgcgagcc caacccgcgc ctcaacttct accccgtatt 24720 tgccgtgcca gaggtgcttg ccacctatca catctttttc caaaactgca agatacccct 24780 atcctgccgt gccaaccgca gccgagcgga caagcagctg gccttgcggc agggcgctgt 24840 catacctgat atcgcctcgc tcaacgaagt gccaaaaatc tttgagggtc ttggacgcga 24900 cgagaagcgc gcggcaaacg ctctgcaaca ggaaaacagc gaaaatgaaa gtcactctgg 24960 agtgttggtg gaactcgagg gtgacaacgc gcgcctagcc gtactaaaac gcagcatcga 25020 ggtcacccac tttgcctacc cggcacttaa cctacccccc aaggtcatga gcacagtcat 25080 gagtgagctg atcgtgcgcc gtgcgcagcc cctggagagg gatgcaaatt tgcaagaaca 25140 aacagaggag ggcctacccg cagttggcga cgagcagcta gcgcgctggc ttcaaacgcg 25200 cgagcctgcc gacttggagg agcgacgcaa actaatgatg gccgcagtgc tcgttaccgt 25260 ggagcttgag tgcatgcagc ggttctttgc tgacccggag atgcagcgca agctagagga 25320 aacattgcac tacacctttc gacagggcta cgtacgccag gcctgcaaga tctccaacgt 25380 ggagctctgc aacctggtct cctaccttgg aattttgcac gaaaaccgcc ttgggcaaaa 25440 cgtgcttcat tccacgctca agggcgaggc gcgccgcgac tacgtccgcg actgcgttta 25500 cttatttcta tgctacacct ggcagacggc catgggcgtt tggcagcagt gcttggagga 25560 gtgcaacctc aaggagctgc agaaactgct aaagcaaaac ttgaaggacc tatggacggc 25620 cttcaacgag cgctccgtgg ccgcgcacct ggcggacatc attttccccg aacgcctgct 25680 taaaaccctg caacagggtc tgccagactt caccagtcaa agcatgttgc agaactttag 25740 gaactttatc ctagagcgct caggaatctt gcccgccacc tgctgtgcac ttcctagcga 25800 ctttgtgccc attaagtacc gcgaatgccc tccgccgctt tggggccact gctaccttct 25860 gcagctagcc aactaccttg cctaccactc tgacataatg gaagacgtga gcggtgacgg 25920 tctactggag tgtcactgtc gctgcaacct atgcaccccg caccgctccc tggtttgcaa 25980 ttcgcagctg cttaacgaaa gtcaaattat cggtaccttt gagctgcagg gtccctcgcc 26040 tgacgaaaag tccgcggctc cggggttgaa actcactccg gggctgtgga cgtcggctta 26100 ccttcgcaaa tttgtacctg aggactacca cgccccacgag attaggttct acgaagacca 26160 atcccgcccg cctaatgcgg agcttaccgc ctgcgtcatt acccagggcc acattcttgg 26220 ccaattgcaa gccatcaaca aagcccgcca agagtttctg ctacgaaagg gacggggggt 26280 ttacttggac ccccagtccg gcgaggagct caacccaatc cccccgccgc cgcagcccta 26340 tcagcagcag ccgcgggccc ttgcttccca ggatggcacc caaaaagaag ctgcagctgc 26400 cgccgccacc cacggacgag gaggaatact gggacagtca ggcagaggag gttttggacg 26460 aggaggagga ggacatgatg gaagactggg agagcctaga cgaggaagct tccgaggtcg 26520 aagaggtgtc agacgaaaca ccgtcaccct cggtcgcatt cccctcgccg gcgccccaga 26580 aatcggcaac cggttccagc atggctacaa cctccgctcc tcaggcgccg ccggcactgc 26640 ccgttcgccg acccaaccgt agatgggaca ccactggaac cagggccggt aagtccaagc 26700 agccgccgcc gttagcccaa gagcaacaac agcgccaagg ctaccgctca tggcgcgggc 26760 acaagaacgc catagttgct tgcttgcaag actgtggggg caacatctcc ttcgcccgcc 26820 gctttcttct ctaccatcac ggcgtggcct tcccccgtaa catcctgcat tactaccgtc 26880 atctctacag cccatactgc accggcggca gcggcagcaa cagcagcggc cacacagaag 26940 caaaggcgac cggatagcaa gactctgaca aagcccaaga aatccacagc ggcggcagca 27000 gcaggaggag gagcgctgcg tctggcgccc aacgaacccg tatcgacccg cgagcttaga 27060 aacaggattt ttcccactct gtatgctata tttcaacaga gcaggggcca agaacaagag 27120 ctgaaaataa aaaacaggtc tctgcgatcc ctcacccgca gctgcctgta tcacaaaagc 27180 gaagatcagc ttcggcgcac gctggaagac gcggaggctc tcttcagtaa atactgcgcg 27240 ctgactctta aggactagtt tcgcgccctt tctcaaattt aagcgcgaaa actacgtcat 27300 ctccagcggc cacacccggc gccagcacct gttgtcagcg ccattatgag caaggaaatt 27360 cccacgccct acatgtggag ttaccagcca caaatgggac ttgcggctgg agctgcccaa 27420 gactactcaa cccgaataaa ctacatgagc gcgggacccc acatgatatc ccgggtcaac 27480 ggaatacgcg cccaccgaaa ccgaattctc ctggaacagg cggctattac caccacacct 27540 cgtaataacc ttaatccccg tagttggccc gctgccctgg tgtaccagga aagtcccgct 27600 cccaccactg tggtacttcc cagagacgcc caggccgaag ttcagatgac taactcaggg 27660 gcgcagcttg cgggcggctt tcgtcacagg gtgcggtcgc ccgggcaggg tataactcac 27720 ctgacaatca gagggcgagg tattcagctc aacgacgagt cggtgagctc ctcgcttggt 27780 ctccgtccgg acgggacatt tcagatcggc ggcgccggcc gctcttcatt cacgcctcgt 27840 caggcaatcc taactctgca gacctcgtcc tctgagccgc gctctggagg cattggaact 27900 ctgcaattta ttgaggagtt tgtgccatcg gtctacttta accccttctc gggacctccc 27960 ggccactatc cggatcaatt tattcctaac tttgacgcgg taaaggactc ggcggacggc 28020 tacgactgaa tgttaagtgg agaggcagag caactgcgcc tgaaacacct ggtccactgt 28080 cgccgccaca agtgctttgc ccgcgactcc ggtgagtttt gctactttga attgcccgag 28140 gatcatatcg agggcccggc gcacggcgtc cggcttaccg cccagggaga gcttgcccgt 28200 agcctgattc gggagtttac ccagcgcccc ctgctagttg agcgggacag gggaccctgt 28260 gttctcactg tgatttgcaa ctgtcctaac cctggattac atcaagatcc tctagttaat 28320 gtcaggtcgc ctaagtcgat taactagagt acccggggat cttattccct ttaactaata 28380 aaaaaaaata ataaagcatc acttacttaa aatcagttag caaatttctg tccagtttat 28440 tcagcagcac ctccttgccc tcctcccagc tctggtattg cagcttcctc ctggctgcaa 28500 actttctcca caatctaaat ggaatgtcag tttcctcctg ttcctgtcca tccgcaccca 28560 ctatcttcat gttgttgcag atgaagcgcg caagaccgtc tgaagatacc ttcaaccccg 28620 tgtatccata tgacacggaa accggtcctc caactgtgcc ttttcttact cctccctttg 28680 tatcccccaa tgggtttcaa gagagtcccc ctggggtact ctctttgcgc ctatccgaac 28740 ctctagttac ctccaatggc atgcttgcgc tcaaaatggg caacggcctc tctctggacg 28800 aggccggcaa ccttacctcc caaaatgtaa ccactgtgag cccacctctc aaaaaaacca 28860 agtcaaacat aaacctggaa atatctgcac ccctcacagt tacctcagaa gccctaactg 28920 tggctgccgc cgcacctcta atggtcgcgg gcaacacact caccatgcaa tcacaggccc 28980 cgctaaccgt gcacgactcc aaacttagca ttgccaccca aggacccctc acagtgtcag 29040 aaggaaagct agccctgcaa acatcaggcc ccctcaccac caccgatagc agtaccctta 29100 ctatcactgc ctcaccccct ctaactactg ccactggtag cttgggcatt gacttgaaag 29160 agcccattta tacacaaaat ggaaaactag gactaaagta cggggctcct ttgcatgtaa 29220 cagacgacct aaacactttg accgtagcaa ctggtccagg tgtgactatt aataatactt 29280 ccttgcaaac taaagttact ggagccttgg gttttgattc acaaggcaat atgcaactta 29340 atgtagcagg aggactaagg attgattctc aaaacagacg ccttatactt gatgttagtt 29400 atccgtttga tgctcaaaac caactaaatc taagactagg acagggccct ctttttataa 29460 actcagccca caacttggat attaactaca acaaaggcct ttacttgttt acagcttcaa 29520 acaattccaa aaagcttgag gttaacctaa gcactgccaa ggggttgatg tttgacgcta 29580 cagccatagc cattaatgca ggagatgggc ttgaatttgg ttcacctaat gcaccaaaca 29640 caaatcccct caaaacaaaa attggccatg gcctagaatt tgattcaaac aaggctatgg 29700 ttcctaaact aggaactggc cttagttttg acagcacagg tgccattaca gtaggaaaca 29760 aaaataatga taagctaact ttgtggacca caccagctcc atctcctaac tgtagactaa 29820 atgcagagaa agatgctaaa ctcactttgg tcttaacaaa atgtggcagt caaatacttg 29880 ctacagtttc agttttggct gttaaaggca gtttggctcc aatatctgga acagttcaaa 29940 gtgctcatct tattataaga tttgacgaaa atggagtgct actaaacaat tccttcctgg 30000 acccagaata ttggaacttt agaaatggag atcttactga aggcacagcc tatacaaacg 30060 ctgttggatt tatgcctaac ctatcagctt atccaaaatc tcacggtaaa actgccaaaa 30120 gtaacattgt cagtcaagtt tacttaaacg gagacaaaac taaacctgta acactaacca 30180 ttacactaaa cggtacacag gaaacaggag acacaactcc aagtgcatac tctatgtcat 30240 tttcatggga ctggtctggc cacaactaca ttaatgaaat atttgccaca tcctcttaca 30300 ctttttcata cattgcccaa gaataaagaa tcgtttgtgt tatgtttcaa cgtgtttatt 30360 tttcaattgc agaaaatttc aagtcatttt tcattcagta gtatagcccc accaccacat 30420 agcttataca gatcaccgta ccttaatcaa actcacagaa ccctagtatt caacctgcca 30480 cctccctccc aacacacaga gtacacagtc ctttctcccc ggctggcctt aaaaagcatc 30540 atatcatggg taacagacat attcttaggt gttatattcc acacggtttc ctgtcgagcc 30600 aaacgctcat cagtgatatt aataaactcc ccgggcagct cacttaagtt catgtcgctg 30660 tccagctgct gagccacagg ctgctgtcca acttgcggtt gcttaacggg cggcgaagga 30720 gaagtccacg cctacatggg ggtagagtca taatcgtgca tcaggatagg gcggtggtgc 30780 tgcagcagcg cgcgaataaa ctgctgccgc cgccgctccg tcctgcagga atacaacatg 30840 gcagtggtct cctcagcgat gattcgcacc gcccgcagca taaggcgcct tgtcctccgg 30900 gcacagcagc gcaccctgat ctcacttaaa tcagcacagt aactgcagca cagcaccaca 30960 atattgttca aaatcccaca gtgcaaggcg ctgtatccaa agctcatggc ggggaccaca 31020 gaacccacgt ggccatcata ccacaagcgc aggtagatta agtggcgacc cctcataaac 31080 acgctggaca taaacattac ctcttttggc atgttgtaat tcaccacctc ccggtaccat 31140 ataaacctct gattaaacat ggcgccatcc accaccatcc taaaccagct ggccaaaacc 31200 tgcccgccgg ctatacactg cagggaaccg ggactggaac aatgacagtg gagagcccag 31260 gactcgtaac catggatcat catgctcgtc atgatatcaa tgttggcaca acacaggcac 31320 acgtgcatac acttcctcag gattacaagc tcctcccgcg ttagaaccat atcccaggga 31380 acaacccatt cctgaatcag cgtaaatccc acactgcagg gaagacctcg cacgtaactc 31440 acgttgtgca ttgtcaaagt gttacattcg ggcagcagcg gatgatcctc cagtatggta 31500 gcgcgggttt ctgtctcaaa aggaggtaga cgatccctac tgtacggagt gcgccgagac 31560 aaccgagatc gtgttggtcg tagtgtcatg ccaaatggaa cgccggacgt agtcatattt 31620 cctgaagcaa aaccaggtgc gggcgtgaca aacagatctg cgtctccggt ctcgccgctt 31680 agatcgctct gtgtagtagt tgtagtatat ccactctctc aaagcatcca ggcgccccct 31740 ggcttcgggt tctatgtaaa ctccttcatg cgccgctgcc ctgataacat ccaccaccgc 31800 agaataagcc acacccagcc aacctacaca ttcgttctgc gagtcacaca cgggaggagc 31860 gggaagagct ggaagaacca tgtttttttt ttattccaaa agattatcca aaacctcaaa 31920 atgaagatct attaagtgaa cgcgctcccc tccggtggcg tggtcaaact ctacagccaa 31980 agaacagata atggcatttg taagatgttg cacaatggct tccaaaaggc aaacggccct 32040 cacgtccaag tggacgtaaa ggctaaaccc ttcagggtga atctcctcta taaacattcc 32100 agcaccttca accatgccca aataattctc atctcgccac cttctcaata tatctctaag 32160 caaatcccga atattaagtc cggccattgt aaaaatctgc tccagagcgc cctccacctt 32220 cagcctcaag cagcgaatca tgattgcaaa aattcaggtt cctcacagac ctgtataaga 32280 ttcaaaagcg gaacattaac aaaaataccg cgatcccgta ggtcccttcg cagggccagc 32340 tgaacataat cgtgcaggtc tgcacggacc agcgcggcca cttccccgcc aggaaccatg 32400 acaaaagaac ccacactgat tatgacacgc atactcggag ctatgctaac cagcgtagcc 32460 ccgatgtaag cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca 32520 ggcaaagcct cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag 32580 gtaagctccg gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt 32640 ttctgcataa acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta 32700 caacaggaaa aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt 32760 aaaaaaactg gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag 32820 tcataatgta agactcggta aacacatcag gttgattcac atcggtcagt gctaaaaagc 32880 gaccgaaata gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca 32940 taggaggtat aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct 33000 gcctaggcaa aatagcaccc tcccgctcca gaacaacata cagcgcttcc acagcggcag 33060 ccataacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac 33120 ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag 33180 gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa 33240 cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt 33300 tcccacgtta cgtcacttcc cattttaaga aaactacaat tcccaacaca tacaagttac 33360 tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc 33420 caccccctca ttatcatatt ggcttcaatc caaaataagg tatattattg atgatg 33476 <210> 38 <211> 6621 <212> DNA <213> Artificial Sequence <220> <223> pShuttle <400> 38 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactggtacc gcggccgcct 360 cgagtctaga gatatcgaat tcaagcttgt cgactcgaag atctgggcgt ggttaagggt 420 gggaaagaat atataaggtg ggggtcttat gtagttttgt atctgttttg cagcagccgc 480 cgccgccatg agcaccaact cgtttgatgg aagcattgtg agctcatatt tgacaacgcg 540 catgccccca tgggccgggg tgcgtcagaa tgtgatgggc tccagcattg atggtcgccc 600 cgtcctgccc gcaaactcta ctaccttgac ctacgagacc gtgtctggaa cgccgttgga 660 gactgcagcc tccgccgccg cttcagccgc tgcagccacc gcccgcggga ttgtgactga 720 ctttgctttc ctgagcccgc ttgcaagcag tgcagcttcc cgttcatccg cccgcgatga 780 caagttgacg gctcttttgg cacaattgga ttctttgacc cgggaactta atgtcgtttc 840 tcagcagctg ttggatctgc gccagcaggt ttctgccctg aaggcttcct cccctcccaa 900 tgcggtttaa aacataaata aaaaaccaga ctctgtttgg atttggatca agcaagtgtc 960 ttgctgtctt tatttagggg ttttgcgcgc gcggtaggcc cgggaccagc ggtctcggtc 1020 gttgagggtc ctgtgtattt tttccaggac gtggtaaagg tgactctgga tgttcagata 1080 catgggcata agcccgtctc tggggtggag gtagcaccac tgcagagctt catgctgcgg 1140 ggtggtgttg tagatgatcc agtcgtagca ggagcgctgg gcgtggtgcc taaaaatgtc 1200 tttcagtagc aagctgattg ccaggggcag gcccttggtg taagtgttta caaagcggtt 1260 aagctgggat gggtgcatac gtggggatat gagatgcatc ttggactgta tttttaggtt 1320 ggctatgttc ccagccatat ccctccgggg attcatgttg tgcagaacca ccagcacagt 1380 gtatccggtg cacttgggaa atttgtcatg tagcttagaa ggaaatgcgt ggaagaactt 1440 ggagacgccc ttgtgacctc caagattttc catgcattcg tccataatga tggcaatggg 1500 cccacgggcg gcggcctggg cgaagatatt tctgggatca ctaacgtcat agttgtgttc 1560 caggatgaga tcgtcatagg ccatttttac aaagcgcggg cggagggtgc cagactgcgg 1620 tataatggtt ccatccggcc caggggcgta gttaccctca cagatttgca tttccccacgc 1680 tttgagttca gatggggggga tcatgtctac ctgcggggcg atgaagaaaa cggtttccgg 1740 ggtaggggag atcagctggg aagaaagcag gttcctgagc agctgcgact taccgcagcc 1800 ggtgggcccg taaatcacac ctattaccgg gtgcaactgg tagttaagag agctgcagct 1860 gccgtcatcc ctgagcaggg gggccacttc gttaagcatg tccctgactc gcatgttttc 1920 cctgaccaaa tccgccagaa ggcgctcgcc gcccagcgat agcagttctt gcaaggaagc 1980 aaagtttttc aacggtttga gaccgtccgc cgtaggcatg cttttgagcg tttgaccaag 2040 cagttccagg cggtcccaca gctcggtcac ctgctctacg gcatctcgat ccagcatatc 2100 tcctcgtttc gcgggttggg gcggctttcg ctgtacggca gtagtcggtg ctcgtccaga 2160 cgggccaggg tcatgtcttt ccacgggcgc agggtcctcg tcagcgtagt ctgggtcacg 2220 gtgaaggggt gcgctccggg ctgcgcgctg gccagggtgc gcttgaggct ggtcctgctg 2280 gtgctgaagc gctgccggtc ttcgccctgc gcgtcggcca ggtagcattt gaccatggtg 2340 tcatagtcca gcccctccgc ggcgtggccc ttggcgcgca gcttgccctt ggaggaggcg 2400 ccgcacgagg ggcagtgcag acttttgagg gcgtagagct tgggcgcgag aaataccgat 2460 tccggggagt aggcatccgc gccgcaggcc ccgcagacgg tctcgcattc cacgagccag 2520 gtgagctctg gccgttcggg gtcaaaaacc aggtttcccc catgcttttt gatgcgtttc 2580 ttacctctgg tttccatgag ccggtgtcca cgctcggtga cgaaaaggct gtccgtgtcc 2640 ccgtatacag acttgagagg gagtttaaac gaattcaata gcttgttgca tgggcggcga 2700 tataaaatgc aaggtgctgc tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac 2760 atcgtagtca tgctcatgca gataaaggca ggtaagctcc ggaaccacca cagaaaaaga 2820 caccattttt ctctcaaaca tgtctgcggg tttctgcata aacacaaaat aaaataacaa 2880 aaaaacattt aaacattaga agcctgtctt acaacaggaa aaacaaccct tataagcata 2940 agacggacta cggccatgcc ggcgtgaccg taaaaaaact ggtcaccgtg attaaaaagc 3000 accaccgaca gctcctcggt catgtccgga gtcataatgt aagactcggt aaacacatca 3060 ggttgattca tcggtcagtg ctaaaaagcg accgaaatag cccgggggaa tacatacccg 3120 caggcgtaga gacaacatta cagcccccat aggaggtata acaaaattaa taggagagaa 3180 aaacacataa acacctgaaa aaccctcctg cctaggcaaa atagcaccct cccgctccag 3240 aacaacatac agcgcttcac agcggcagcc taacagtcag ccttaccagt aaaaaagaaa 3300 acctattaaa aaaacaccac tcgacacggc accagctcaa tcagtcacag tgtaaaaaag 3360 ggccaagtgc agagcgagta tatataggac taaaaaatga cgtaacggtt aaagtccaca 3420 aaaaacaccc agaaaaccgc acgcgaacct acgcccagaa acgaaagcca aaaaacccac 3480 aacttcctca aatcgtcact tccgttttcc cacgttacgt aacttcccat tttaagaaaa 3540 ctacaattcc caacacatac aagttactcc gccctaaaac ctacgtcacc cgccccgttc 3600 ccacgccccg cgccacgtca caaactccac cccctcatta tcatattggc ttcaatccaa 3660 aataaggtat attattgatg atgttaatta acatgcatgg atccatatgc ggtgtgaaat 3720 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac 3780 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 3840 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 3900 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 3960 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 4020 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 4080 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 4140 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 4200 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 4260 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 4320 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 4380 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 4440 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 4500 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 4560 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 4620 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtata 4680 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 4740 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 4800 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 4860 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 4920 aactttatcc gcctccatcc agtctattataa ttgttgccgg gaagctagag taagtagttc 4980 gccagttaat agtttgcgca acgttgttgc cattgctgca gccatgagat tatcaaaaag 5040 gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg 5100 gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca 5160 ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag 5220 cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa 5280 ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga 5340 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcaccgcag gttctccggc 5400 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 5460 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 5520 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac 5580 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 5640 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 5700 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 5760 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 5820 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 5880 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 5940 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 6000 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 6060 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 6120 catcgccttc tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa 6180 tcagctcatt ttttaaccaa taggccgaaa tcggcaacat cccttataaa tcaaaagaat 6240 agaccgcgat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 6300 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 6360 catcacccaa atcaagtttt ttgcggtcga ggtgccgtaa agctctaaat cggaacccta 6420 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 6480 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 6540 taaccaccac acccgcgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg 6600 atcgaattaa ttcttaatta a 6621 <210> 39 <211> 7469 <212> DNA <213> Artificial Sequence <220> <223> pShuttle-CMV <400> 39 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt 360 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 420 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 480 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 540 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 600 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 660 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 720 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 780 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 840 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 900 agagctggtt tagtgaaccg tcagatccgc tagagatctg gtaccgtcga cgcggccgct 960 cgagcctaag cttctagata agatatccga tccaccggat ctagataact gatcataatc 1020 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 1080 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 1140 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 1200 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcggatctgg gcgtggttaa 1260 gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag 1320 ccgccgccgc catgagcacc aactcgtttg atggaagcat tgtgagctca tatttgacaa 1380 cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat gggctccagc attgatggtc 1440 gccccgtcct gcccgcaaac tctactacct tgacctacga gaccgtgtct ggaacgccgt 1500 tggagactgc agcctccgcc gccgcttcag ccgctgcagc caccgcccgc gggattgtga 1560 ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc ttcccgttca tccgcccgcg 1620 atgacaagtt gacggctctt ttggcacaat tggattcttt gacccgggaa cttaatgtcg 1680 tttctcagca gctgttggat ctgcgccagc aggtttctgc cctgaaggct tcctcccctc 1740 ccaatgcggt ttaaaacata aataaaaaac cagactctgt ttggatttgg atcaagcaag 1800 tgtcttgctg tctttattta ggggttttgc gcgcgcggta ggcccgggac cagcggtctc 1860 ggtcgttgag ggtcctgtgt attttttcca ggacgtggta aaggtgactc tggatgttca 1920 gatacatggg cataagcccg tctctggggt ggaggtagca ccactgcaga gcttcatgct 1980 gcggggtggt gttgtagatg atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa 2040 tgtctttcag tagcaagctg attgccaggg gcaggccctt ggtgtaagtg tttacaaagc 2100 ggttaagctg ggatgggtgc atacgtgggg atatgagatg catcttggac tgtattttta 2160 ggttggctat gttcccagcc atatccctcc ggggattcat gttgtgcaga accaccagca 2220 cagtgtatcc ggtgcacttg ggaaatttgt catgtagctt agaaggaaat gcgtggaaga 2280 acttggagac gcccttgtga cctccaagat tttccatgca ttcgtccata atgatggcaa 2340 tgggcccacg ggcggcggcc tgggcgaaga tatttctggg atcactaacg tcatagttgt 2400 gttccaggat gagatcgtca taggccattt ttacaaagcg cgggcggagg gtgccagact 2460 gcggtataat ggttccatcc ggcccagggg cgtagttacc ctcacagatt tgcatttccc 2520 acgctttgag ttcagatggg gggatcatgt ctacctgcgg ggcgatgaag aaaacggttt 2580 ccggggtagg ggagatcagc tgggaagaaa gcaggttcct gagcagctgc gacttaccgc 2640 agccggtggg cccgtaaatc acacctatta ccggctgcaa ctggtagtta agagagctgc 2700 agctgccgtc atccctgagc aggggggcca cttcgttaag catgtccctg actcgcatgt 2760 tttccctgac caaatccgcc agaaggcgct cgccgcccag cgatagcagt tcttgcaagg 2820 aagcaaagtt tttcaacggt ttgagaccgt ccgccgtagg catgcttttg agcgtttgac 2880 caagcagttc caggcggtcc cacagctcgg tcacctgctc tacggcatct cgatccagca 2940 tatctcctcg tttcgcgggt tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc 3000 cagacgggcc agggtcatgt ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt 3060 cacggtgaag gggtgcgctc cgggctgcgc gctggccagg gtgcgcttga ggctggtcct 3120 gctggtgctg aagcgctgcc ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat 3180 ggtgtcatag tccagcccct ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga 3240 ggcgccgcac gaggggcagt gcagactttt gagggcgtag agcttgggcg cgagaaatac 3300 cgattccggg gagtaggcat ccgcgccgca ggccccgcag acggtctcgc attccacgag 3360 ccaggtgagc tctggccgtt cggggtcaaa aaccaggttt cccccatgct ttttgatgcg 3420 tttcttacct ctggtttcca tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt 3480 gtccccgtat acagacttga gagggagttt aaacgaattc aatagcttgt tgcatgggcg 3540 gcgatataaa atgcaaggtg ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa 3600 gcacatcgta gtcatgctca tgcagataaa ggcaggtaag ctccggaacc accacagaaa 3660 aagacaccat ttttctctca aacatgtctg cgggtttctg cataaacaca aaataaaata 3720 acaaaaaaac atttaaacat tagaagcctg tcttacaaca ggaaaaacaa cccttataag 3780 cataagacgg actacggcca tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa 3840 aagcaccacc gacagctcct cggtcatgtc cggagtcata atgtaagact cggtaaacac 3900 atcaggttga ttcacatcgg tcagtgctaa aaagcgaccg aaatagcccg ggggaataca 3960 tacccgcagg cgtagagaca acattacagc ccccatagga ggtataacaa aattaatagg 4020 agagaaaaac acataaacac ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg 4080 ctccagaaca acatacagcg cttccacagc ggcagccata acagtcagcc ttaccagtaa 4140 aaaagaaaac ctattaaaa aacaccactc gacacggcac cagctcaatc agtcacagtg 4200 taaaaaaggg ccaagtgcag agcgagtata tataggacta aaaaatgacg taacggttaa 4260 agtccacaaa aaacacccag aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa 4320 aaacccacaa cttcctcaaa tcgtcacttc cgttttccca cgttacgtca cttcccattt 4380 taagaaaact acaattccca acacatacaa gttactccgc cctaaaacct acgtcacccg 4440 ccccgttccc acgccccgcg ccacgtcaca aactccaccc cctcattatc atattggctt 4500 caatccaaaa taaggtatat tattgatgat gttaattaac atgcatggat ccatatgcgg 4560 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 4620 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 4680 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 4740 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 4800 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 4860 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 4920 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 4980 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 5040 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 5100 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 5160 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 5220 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 5280 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 5340 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 5400 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 5460 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 5520 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 5580 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 5640 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 5700 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 5760 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 5820 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagc catgagatta 5880 tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc 5940 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag 6000 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg 6060 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc 6120 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct 6180 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 6240 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 6300 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 6360 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 6420 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 6480 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 6540 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 6600 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 6660 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 6720 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 6780 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 6840 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 6900 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 6960 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt 7020 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaccatcc cttataaatc 7080 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 7140 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 7200 acgtgaacca tcaccctaat caagtttttt gtggtcgagg tgccgtaaag cactaaatcg 7260 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 7320 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 7380 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc 7440 cattcaggat cgaattaatt cttaattaa 7469 <210> 40 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 40 ggtctatata agcagagctg 20 <210> 41 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 41 gtggtatggc tgattatgat cag 23

Claims (9)

An adenovirus vector in which bases at positions corresponding to positions 7275 to 7881 and 8583 to 9630 in the E2B region in the sequence of SEQ ID NO: 1 are deleted.
The adenoviral vector according to claim 1, wherein the deletion causes inactivation of a DNA polymerase gene (pol) and a preterminal protein gene (pTP).
The adenoviral vector according to claim 1, wherein the adenoviral vector has bases deleted at positions corresponding to positions 7274 to 7881 and 8583 to 9630 in the sequence of SEQ ID NO: 1.
The adenoviral vector according to claim 1, wherein the adenoviral vector has a base deleted at positions corresponding to positions 7275 to 9632 in the sequence of SEQ ID NO: 1.
The adenoviral vector according to claim 1, wherein a foreign gene is introduced into at least a part of the deletion site.
A method for producing an adenoviral vector comprising the step of infecting a packaging cell line with the adenoviral vector of any one of claims 1 to 5.
The method of claim 6, wherein the packaging cell line is HEK-293 (Human embryonic kidney 293), HEK-293-derived cells, 911, PER.C6, A549 (carcinomic human alveolar basal epithelial cell), HER (Human Embryonic Retinoblast), HEL 299 (Human lung fibroblast), HeLa (Human cervical cancer cell), CHO (Chinese hamster ovary cell), BHK (Baby hamster kidney cell), 3T3 (Mouse embryonic fibroblast cell), WS1 (Human dermal fibroblast), MRC5 (Human Lung Fibroblast), WI38 (Human lung fibroblast cell), AE1-2a (Human lung adenocarcinoma), PC12 (Rat Pheochromocytoma cell), Primary human amniocytes, African green monkey kidney cells, and any one selected from the group consisting of Cocker Spaniel kidney cells Phosphorus, manufacturing method.
The method according to claim 7, wherein the packaging cell line expresses a DNA polymerase gene (pol) and a preterminal protein gene (pTP).
A cell transformed with the adenoviral vector of any one of claims 1 to 5.

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