KR20210091996A - Gamma-Hydroxybutyric Acid Detection Kit and Manufacturing method thereof - Google Patents

Abstract

The present invention relates to a detection kit including fibers coated with a gamma-hydroxybutyric acid detection material and a method for manufacturing the same, and more particularly, to a detection kit including fibers coated with a gamma-hydroxybutyric acid detection material and a method for manufacturing the same, in which it is possible to quickly and conveniently detect gamma-hydroxybutyric acid by adsorbing a gamma-hydroxybutyric acid detection material capable of identifying the presence or absence of gamma-hydroxybutyric acid according to a color change on the fiber when gamma-hydroxybutyric acid is included therein, and excellent portability is also provided. The method for manufacturing a detection kit including a fiber coated with a gamma-hydroxybutyric acid detection material according to the present invention comprises: a detection solution preparation step of preparing a detection solution by mixing a gamma-hydroxybutyric acid detection material and a solvent (S100) and an immersion step of immersing the fiber in the detection solution (S200).

Description

A detection kit comprising fibers coated with a gamma-hydroxybutyric acid detection material and a manufacturing method thereof {Gamma-Hydroxybutyric Acid Detection Kit and Manufacturing method thereof}

The present invention relates to a detection kit comprising a fiber coated with a gamma-hydroxybutyric acid sensing material and a method for manufacturing the same, and more particularly, to gamma-hydroxybutyric acid by color change when gamma-hydroxybutyric acid is included. By adsorbing a gamma-hydroxybutyric acid detection material that can confirm the presence or absence of a gamma-hydroxybutyric acid detection material to the fiber, it is possible to quickly and conveniently detect gamma-hydroxybutyric acid, including fibers coated with a gamma-hydroxybutyric acid detection material with excellent portability. It relates to a detection kit and a method for manufacturing the same.

GHB (Gamma-HydroxyButyric acid) is a central nervous system depressant and has been used as a general anesthetic in the 1960s and 1970s. After that, it has been used for various purposes such as sleep disorders, depression treatment, fat reduction and promotion of muscle development. However, it is known to cause drowsiness, hallucinations, and dizziness, and has been used for crimes in the United States since the 1990s, and was designated and banned as a controlled substance in 2000. Korea was designated as Psychotropic Substances in 2001.

GHB is mixed with food and beverage and used for crime. It is colorless and odorless and difficult to detect. Even when absorbed into the body, the highest concentration is reached within 30 minutes in the blood and within 1 hour in the urine. Since the detection time is short, it is difficult to confirm whether or not GHB is ingested unless it is immediately confirmed.

As a patent document related to GHB detection, Korean Patent Publication No. 10-2012-0015327 discloses a sample diluted by adding an oxalic acid-containing buffer combined with EDTA, and GHB to succinate semialdehyde (SSA) by reducing oxidative cofactors. gamma-hydroxy butyric acid (GHB) in a sample comprising incubating an enzyme capable of a method for measuring the concentration of and an enzyme capable of converting GHB to succinic semialdehyde (SSA), an oxidation cofactor and oxalic acid having a concentration range of 1 to 100 mM, preferably 10 mM, and a concentration range of 0.1 to 1.5 mM, preferably discloses a composition for assaying a sample for GHB and a kit using the same, comprising a buffer containing 0.8 mM EDTA.

Currently, most of the GHB assay reagents used in Korea rely on imported detection kits to detect and confirm them in urine, blood, and hair, and they are expensive and unsatisfactory in terms of accuracy.

As part of the research to develop a detection kit that can easily and quickly check the presence or absence of detection of gamma-hydroxybutyric acid according to the user's needs, the present inventors adsorb the gamma-hydroxybutyric acid detection material to the fiber for portability. This is excellent, and by absorbing a suspected substance containing gamma-hydroxybutyric acid in the fiber, a kit that can confirm the presence or absence of gamma-hydroxybutyric acid by color change was prepared, and the possibility of using it as a detection and detection kit was improved. Confirmation led to the present invention.

Korean Patent Publication No. 10-2012-0015327 (Method, composition and kit suitable for measuring the concentration of gamma-hydroxybutyric acid (GHB) in a sample)

An object of the present invention to solve the above problems is to quickly and quickly adsorb a gamma-hydroxybutyric acid sensing material capable of confirming the presence or absence of gamma-hydroxybutyric acid by color change when gamma-hydroxybutyric acid is included on the fiber. It is an object to provide a detection kit including a fiber coated with a gamma-hydroxybutyric acid sensing material, which can easily detect gamma-hydroxybutyric acid and has excellent portability, and a method for manufacturing the same.

To solve the above problems, there is provided a detection kit comprising fibers coated with a gamma-hydroxybutyric acid sensing material of the present invention.

In order to solve the above problems, the method for manufacturing a detection kit including a fiber coated with a gamma-hydroxybutyric acid sensing material of the present invention is a sensing solution prepared by mixing a gamma-hydroxybutyric acid sensing material and a solvent It includes a manufacturing step (S100) and an immersion step (S200) of immersing the fiber in the sensing solution.

As the fiber, any one of linen, nylon, and a combination thereof is used.

As described above, according to the sensing kit including the fiber coated with the gamma-hydroxybutyric acid sensing material and the manufacturing method thereof according to the present invention, when gamma-hydroxybutyric acid is included, the gamma-hydroxybutyric acid is changed by color change. By adsorbing a gamma-hydroxybutyric acid sensing material that can check the presence or absence of ric acid to the fiber, it is possible to quickly and easily detect gamma-hydroxybutyric acid, and has excellent portability.

1 is a flow chart showing a method of manufacturing a detection kit including a fiber coated with a gamma-hydroxybutyric acid detection material of the present invention.
Figure 2 confirms the reaction with GHB Orange of GABA, an analog of GHB, (A) is a 25mM GHB Orange solution under visible light, (B) is a 25mM GHB Orange solution under UV light, (C) is 25mM under visible light A mixture of GHB Orange solution and GABA solution and (D) show a mixture of 25 mM GHB Orange solution and GABA solution under UV light.
3 is a comparison of the degree of dyeing with GHB Orange according to the type of fiber.
Figure 4 shows the change according to the concentration of the detection solution and the number of staining days, (A) shows the fluorescence intensity under visible light, (B) shows the fluorescence intensity under UV light.
Figure 5 shows the change in fluorescence when immersed in the GABA solution in linen yarn treated with the sensing solution, (A) shows the fluorescence intensity under visible light, (B) shows the fluorescence intensity under UV light.
6 is an observation of the color of the filtrate after dyeing 7 types of fibers in the fiber selection experiment, and shows the dyeing filtrate of 80 cotton, 40 cotton, and nylon from the left.

Specific features and advantages of the present invention will be described in detail below with reference to the accompanying drawings. Prior to this, if it is determined that the detailed description of the function and its configuration related to the present invention may unnecessarily obscure the gist of the present invention, the detailed description will be omitted.

The present invention relates to a detection kit comprising a fiber coated with a gamma-hydroxybutyric acid sensing material and a method for manufacturing the same, and more particularly, to gamma-hydroxybutyric acid by color change when gamma-hydroxybutyric acid is included. By adsorbing a gamma-hydroxybutyric acid detection material that can confirm the presence or absence of a gamma-hydroxybutyric acid detection material to the fiber, it is possible to quickly and conveniently detect gamma-hydroxybutyric acid, including fibers coated with a gamma-hydroxybutyric acid detection material with excellent portability. It relates to a detection kit and a method for manufacturing the same.

1 is a flowchart showing a method of manufacturing a detection kit including a fiber coated with a gamma-hydroxybutyric acid detection material of the present invention.

According to the present invention, the method for manufacturing a detection kit comprising a fiber coated with a gamma-hydroxybutyric acid detection material includes a detection solution preparation step (S100) of mixing a gamma-hydroxybutyric acid detection material and a solvent to prepare a detection solution and an immersion step (S200) of immersing the fiber in the sensing solution.

The detection solution preparation step (S100) is a step of preparing a detection solution by mixing a gamma-hydroxybutyric acid detection material and a solvent, and GHB Orange is used as the detection material for gamma-hydroxybutyric acid (hereinafter abbreviated as GHB). do.

GHB Orange causes fluorescence quenching upon reaction with GHB. More specifically, the mechanism of operation of GHB Orange is when hydrogen bonds with the carboxyl group of GHB and the hydroxyl group of GHB orange, the electrons in GHB ride hydrogen bonds to GHB By increasing the electron concentration of Orange to cause a decrease in fluorescence, the presence or absence of GHB detection can be confirmed by visually observing the presence or absence of fluorescence quenching and color change.

The detection solution may have a concentration of 5 to 100 mM, and as the solvent for forming the detection solution, any one of distilled water, an organic solvent, and a combination thereof may be used. Preferably, distilled water and ethanol are 1: A mixed solvent mixed in a volume ratio of 1 may be used.

In the immersion step (S200), the fiber is immersed in the sensing solution to adsorb the sensing material to the fiber. The fiber may be any one of linen, nylon, and a combination thereof, and may be dried after immersion treatment for 12 hours to 10 days.

More preferably, the fiber may be subjected to a surface modification treatment to remove and reduce hydroxyl groups on the surface, and the surface modification treatment may include any one of hot air drying, hydrophobic modification, and a combination thereof. By minimizing the hydroxyl group of the fiber, the carboxyl group of GHB can improve the hydroxyl group and hydrogen bonding properties of the GHB sensing material.

Hereinafter, a sensing kit including fibers coated with a gamma-hydroxybutyric acid sensing material according to the present invention will be described.

The detection kit including fibers coated with a gamma-hydroxybutyric acid sensing material according to the present invention is a gamma-hydroxybutyric acid capable of confirming the presence or absence of gamma-hydroxybutyric acid by color change when gamma-hydroxybutyric acid is included. By adsorbing the ric acid sensing material to the fiber, it is possible to quickly and simply detect gamma-hydroxybutyric acid, and has excellent portability.

GHB Orange is used as the sensing material for gamma-hydroxybutyric acid (hereinafter, abbreviated as GHB), and GHB Orange causes fluorescence quenching when reacting with GHB. More specifically, the operating mechanism of GHB Orange is the carboxyl of GHB. When a group and a hydroxyl group of GHB orange are hydrogen-bonded, electrons in GHB ride hydrogen bonds and increase the electron concentration of GHB Orange to cause a decrease in fluorescence. GHB is detected by visually observing the presence or absence of fluorescence quenching and color change. presence can be checked.

In order to adsorb the GHB sensing material to the fiber, a sensing solution is prepared and the fiber is immersed. At this time, the sensing solution may have a concentration of 5 to 100 mM, and the solvent for forming the sensing solution is distilled water, organic Any one of a solvent and a combination thereof may be used, and preferably, a mixed solvent in which distilled water and ethanol are mixed in a volume ratio of 1:1 may be used.

The fiber may be any one of linen, nylon, and a combination thereof, and may be dried after immersion treatment for 12 hours to 10 days.

More preferably, the fiber may be subjected to a surface modification treatment to remove and reduce hydroxyl groups on the surface, and the surface modification treatment may include any one of hot air drying, hydrophobic modification, and a combination thereof. By minimizing the hydroxyl group of the fiber, the carboxyl group of GHB can improve the hydroxyl group and hydrogen bonding properties of the GHB sensing material.

The sensing kit according to the present invention is provided in a form in which the fiber coated with the gamma-hydroxybutyric acid sensing material is accommodated inside the bracelet, pad, and inhalation container, so it is excellent in portability, The presence or absence of hydroxybutyric acid can be confirmed.

More specifically, in the case of the inhalation container, the fiber coated with the gamma-hydroxybutyric acid sensing material is stored therein, and the gamma-hydroxybutyric acid sensing target liquid is sucked into the inside of the gamma-hydroxybutyric acid sensing material by the principle of dropper suction. -The change in fluorescence quenching can be confirmed by allowing the hydroxybutyric acid sensing material to react with the liquid to be detected.

A valve-shaped structure may be provided in the passage through which the liquid to be sensed is introduced to prevent the liquid to be sensed flowing into the inside from being discharged to the outside.

Hereinafter, the present invention will be described in detail below with reference to a preferred embodiment. However, the following examples are intended to specifically illustrate the present invention, and are not limited thereto.

1. Experimental verifiability of GHB analogues

Since it is difficult to obtain GHB, an experiment was performed using GABA, which is an analog of GHB, and the fluorescence quenching effect was confirmed by mixing GHB Orange solution and GABA to confirm the possibility of experimental verification.

A 25 mM GHB Orange solution was prepared using a solvent mixed with distilled water: ethanol in a volume ratio of 1: 1, and a GABA solution having 250 mg/ml was prepared, but ethanol was added to have an ethanol content similar to that of a commercially available beverage. Changes according to concentrations (0%, 5%, 40%) were confirmed.

Figure 2 confirms the reaction with GHB Orange of GABA, an analog of GHB, (A) is a 25mM GHB Orange solution under visible light, (B) is a 25mM GHB Orange solution under UV light, (C) is 25mM under visible light A mixture of GHB Orange solution and GABA solution and (D) shows a mixture of 25 mM GHB Orange solution and GABA solution under UV light.

It can be seen that there is a clear difference in visible light and UV light before/after adding the GABA solution. Therefore, it was found that GABA - GHB Orange reacted in the liquid phase, but it was difficult to visually observe the degree of fluorescence quenching according to the content of ethanol. As a result, fluorescence quenching of GHB Orange occurred even using GABA, which is an analogue, not GHB, and it was determined that experimental verification would be possible.

2. Fiber Selection

Linen, wool, 40 cotton, 80 cotton, nylon, Poly DTY, and Poly spun yarns were prepared in order to select the optimal fiber for use in the detection of GHB by adsorbing GHB Orage.

3 is a comparison of the degree of dyeing with GHB Orange according to the type of fiber.

In the case of cotton fibers, some activity was shown when dyed with GHB Orange, but only when irradiated with UV light, cotton was excluded. A test was performed using a GABA solution, but no reaction occurred. Therefore, it was excluded from subsequent experiments.

In the case of nylon, the dyeing in nylon was the best, and when the dyeing solution after dyeing was observed, it was found that GHB Orange moved to nylon well enough to turn white. Fluorescence was not lost when tested using a GABA solution, so it was excluded from subsequent experiments.

In the case of linen, when dyeing was performed using GHB Orange, a pink color appeared when observed with the naked eye, and a strong orange fluorescence was emitted when irradiated with UV light. When the test was performed using a GABA solution, it was observed that a decrease in fluorescence occurred to some extent. Therefore, it was decided to proceed with GHB Orange staining using linen.

3. Visual change by concentration of detection solution and number of days of staining

GHB Orange solution was prepared with 25mM, 50mM, and 100mM, and linen samples that were dyed for 1, 3 and 5 days were prepared to check the degree of staining according to the number of staining days. In addition, the degree of dyeing according to the drying method was also confirmed.

Figure 4 shows the change according to the concentration of the detection solution and the number of staining days, (A) shows the fluorescence intensity under visible light, (B) shows the fluorescence intensity under UV light. In addition, the degree of dyeing according to the drying method was indicated as 'not' when the solution was not washed and dried, and 'wiped' when the solution was cleaned and dried. As a result, it was confirmed that the color according to the dyeing time, concentration, and drying method was slightly different in the visible light, but the fluorescence intensity was hardly different.

Figure 5 shows the change in fluorescence when immersed in the GABA solution on linen yarn treated with the sensing solution, (A) shows the fluorescence intensity under visible light, (B) shows the fluorescence intensity under UV light.

As a result, it was possible to observe that there was no significant change in fluorescence in almost all samples,

Therefore, it was decided to establish a hypothesis and test the reason why the fluorescence reduction of GHB Orange did not occur in linen.

The mechanism of operation of GHB Orange is that when hydrogen bonds with the carboxyl group of GHB and the hydroxyl group of GHB orange, electrons in GHB ride hydrogen bonds to increase the electron concentration of GHB Orange, resulting in a decrease in fluorescence. Therefore, it was hypothesized that the phenomenon that the fluorescence reduction of GHB Orange did not occur even though the GABA solution was added did not increase the electron concentration of GHB Orange attached to the linen.

The conclusion of testing the hypothesis based on the chemical structure of linen is that -OH group present in linen first bonds with the carboxyl group of GABA, so that GABA cannot reach GHB Orange' or 'The concentration of electrons in GHB Orange is caused by linen. Two hypotheses were established: GABA does not supply enough electrons to cause fluorescence quenching even if GABA supplies electrons to GHB Orange. In other words, it is concluded that the electron exchange between GABA-GHB Orange is weakened by linen and disturbs the sensitivity of the fluorescent sensor.

6 shows the color of the filtrate after dyeing 7 types of fibers in the fiber selection experiment, and shows the dyeing filtrate of 80 cotton, 40 cotton, and nylon from the left.

In 80 cotton water, it has the same color as the initial solution, but in GHB Orange solution and 40 cotton water, the color of the filtrate changed to purple like a solution reacted with GABA even though it was in contact with each other. A change in concentration could be observed.

Through this experiment, it is possible to verify the activity of GHB Orange and its reactivity with GABA in various fibers, thereby providing basic knowledge for the manufacture of fluorescent detecting products using GHB Orange. Through this, it was expected that information on material selection could be provided when manufacturing products using materials, thereby speeding up product development in the future.

As described above, the present invention has been mainly described with reference to the accompanying drawings, but those of ordinary skill in the art to which the present invention pertains within the scope not departing from the technical spirit and scope described in the claims of the present invention. Various modifications or variations of the present invention can be practiced. Accordingly, the scope of the present invention should be construed by the appended claims including examples of many such modifications.

Claims (4)

A detection kit comprising a fiber coated with a gamma-hydroxybutyric acid sensing material capable of confirming the presence or absence of gamma-hydroxybutyric acid by fluorescence quenching.
The method of claim 1,
The fiber is characterized in that any one of linen, nylon, and a combination thereof is used.
A detection kit comprising fibers coated with a gamma-hydroxybutyric acid detection material.
A sensing solution preparation step (S100) of preparing a sensing solution by mixing a gamma-hydroxybutyric acid sensing material and a solvent;
characterized in that it comprises an immersion step (S200) of immersing the fiber in the sensing solution
A method for manufacturing a detection kit comprising fibers coated with a gamma-hydroxybutyric acid detection material.
4. The method of claim 3,
The fiber is characterized in that any one of linen, nylon, and a combination thereof is used.
A method for manufacturing a detection kit comprising fibers coated with a gamma-hydroxybutyric acid detection material.

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