KR20210070699A - Cognitive improvable Oggo obtained from fermented and extracted mixture of Red ginseng, Polygala tenuifolia, Poliacocos, and Rehmanneh Glutinosa - Google Patents

Abstract

The present invention relates to a composition for improving cognitive function or memory ability, comprising, as an active ingredient, a fermented mixture of red ginseng, Polygala tenuifolia, Stachys sieboldii, morel and Wolfiporia extensa. Since the fermented mixture of red ginseng, Polygala tenuifolia, morel, Stachys sieboldii and Wolfiporia extensa has an excellent effect of inhibiting the activity of acetylcholinease, which inhibits neurotransmission by destroying acetylcholine and an excellent recovery ability for memory and cognitive ability for the scopolamine-induced memory-impaired animal model, the composition can be used for the prevention or treatment of degenerative cranial nerve diseases such as Alzheimer's dementia, and used as a health functional food composition for alleviating reduced memory, and improving or enhancing learning ability and cognitive function.

Description

Cognitive improvable Oggo obtained from fermented and extracted mixture of Red ginseng, Polygala tenuifolia, Poliacocos, and Rehmanneh Glutinosa}

The present invention relates to an okgo for improving cognitive ability containing a mixed fermented product of red ginseng, raw ginseng, bokryeong, morel and choseokjam as an active ingredient.

With the development of medical technology and the improvement of living standards, the average life expectancy of Koreans has been extended by 1.5 times over the past half century, which has resulted in a rapid increase in the proportion of the elderly population to the total population. According to the 2005 data released by the National Statistical Office, the average life expectancy of Koreans is 78.5 years old, and in 2000, the proportion of the population over 65 years old reached about 7%, indicating that Korea is rapidly entering an aging society. In addition, Korea has already entered an aged society in 2018, and it is predicted that by 2026, it will enter a super-aged society in which the proportion of the elderly population is more than 20%. In addition, the old-age dependency ratio (the number of people aged 65 and over/the number of people aged 15 to 64), which was 12.1% in 2004, is expected to rise to 21.3% in 2020 and 35.7% in 2030. If one elderly person was supported for every 8.2 working-age population in 2004, this would be equivalent to supporting one senior for every 6.8 people in 2010, one senior for every 4.7 people in 2020, and one senior for every 2.8 people in 2030. This means that the possibility of social burden due to a decrease in national economic growth, a decrease in national competitiveness, and an intensification of intergenerational conflict is increasing.

As if reflecting these social prospects and realities, recently, attention has been focused on diseases that occur a lot in the elderly. In particular, it is known that the incidence rate of dementia increases with age, and the incidence rate is about 20% for the elderly aged 75 to 84 and about 50% for the elderly over 85 years of age. Due to the nature of the disease, it is absolutely dependent on the care of others. Therefore, it is emerging as a major social concern. In addition, the amount of economic loss due to dementia is very large, and considering all matters such as labor loss, the social cost of dementia treatment in Korea is estimated to be 3.4 trillion to 4.4 trillion won. In the United States, it is estimated that the cost of treatment for dementia is between $100 billion and $120 billion annually. In other words, as society is gradually aging, senile dementia is emerging as the biggest health problem to be solved by mankind in the 21st century. Accordingly, there is a growing demand for the development of functional substances and foods capable of preventing and treating cognitive disorders including dementia and depression.

Cognitive dysfunction, such as loss of memory and learning ability caused by senile dementia, is caused by damage to acetylcholine neurons at the base of the brain. Drugs that can enhance the function of acetylcholinergic neurons have been developed according to various mechanisms of action, such as agonists, acetylcholine production promoters, and acetylcholinesterase (AChE) inhibitors.

In fact, most Alzheimer's disease drugs currently used in countries are acetylcholinesterase inhibitors, and tacrine and donepezil are also included. In addition, rivastigmin and galatamine are used.

At present, interest in the development of more effective agents (including health functional food ingredients) for the improvement of cognitive or memory deterioration as a degenerative cranial nerve disease is higher than ever. Ultimately, there is a need for research on the development of materials with novel multi-functional effects targeting the mechanisms of cranial nerve cells for the onset of dementia.

On the other hand, ginseng is generally divided into white ginseng and red ginseng according to the processing method. White ginseng is unprocessed ginseng mined in the field, that is, fresh ginseng is dried as it is, and red ginseng is dried and processed by steaming fresh ginseng and dried ginseng. Numerous chemical changes such as amino acid changes and browning are involved. In particular, the heat generated during the manufacturing process of red ginseng creates new ingredients such as ginsenosides Rg2, Rg3, Rhl, and Rh2, which are unique ingredients of red ginseng that do not exist in ginseng. It is known to be involved in action, cancer cell growth inhibitory action, blood pressure lowering action, brain nerve cell protection and learning ability improvement action, antithrombotic action, and antioxidant action.

Polygalatenuifolia willd is a perennial grass of the order Asteraceae, which is used by drying the roots. It has a warm property with a spicy and bitter taste. It is known to have expectorant, sedative, and hypnotic effects, and is widely prescribed for forgetfulness, neurasthenia, palpitations and insomnia. The efficacy of this base paper has been studied in various fields, and neuroprotective effects on the central nervous system, learning ability improvement effects, anti-stress effects, and antidepressant effects have been reported.

Bokryeong is a fungus mass of the fungus and plant parasitic on the root of an evergreen arboreal pine tree. It is parasitic on the roots of pine trees growing in the mountains of Korea, and is distributed in Anhui and Yunnan, China. The sclerotia that are always seen in the oyster mushroom are mostly irregular mass, spherical, flat, oval, and long oval, and there are several types of different sizes. According to the color of the interior, it is divided into Baekbok-ryeong and Jeokbok-ryeong. In general, it is known that Jeokbokryeong is Yi-seup, Baekbokryeong is Geonbi, and Bokshin is not believed, but recently, Jeokbokryeong and Baekbokryeong are not divided and all are called Bokryeong. Bokryeong has a diuretic effect, lowering blood sugar, and relaxing effect on the digestive system.

On the other hand, Korean Patent No. 10-1304707 discloses 'a composition for improving cognitive function containing ginseng as an active ingredient', and Korean Patent Publication No. 10-2017-0054157 discloses 'a composition containing ginseng extract as an active ingredient'. A composition for improving cognitive function and a method for preparing the same are disclosed.

However, there is no disclosure about the 'composition for improving cognitive function or depression using the "mixed fermented product" of red ginseng, raw ginseng, morel, choseokjam and bokryeong as an active ingredient of the present invention. In addition, the specific experimental method (animal test) for the effect of cognitive function was not adequately explained.

In the present invention, the technology of fermenting raw paper, morel, choseokjam and bokryeong together with red ginseng as a complex strain was applied. In addition, the antioxidant effect of the obtained fermented extract was measured and compared, and the cognitive function improvement effect was confirmed by a mouse maze experiment.

Korea Patent Publication No. 10-1304707 (2013.09.06 Announcement) Korean Patent Publication No. 10-2017-0054157 (published on May 17, 2017)

The present invention has been derived by the above requirements, and in the present invention, Lactobacillus plantarum , Lactobacillus buchneri , and Lactobacillus rharmnosus strains are combined to produce red ginseng, raw paper, When a mixture of Morel, Choseokjam and Bokryeong was fermented, compared to the case of using the above three strains respectively, the inhibitory effect of acetylcholine degrading enzyme (acetylchol, nesterase) was excellent, and due to scopolamine induction, The present invention was completed by confirming that the reduced mouse model exhibited remarkable recovery ability for cognitive function and memory.

In order to solve the above problems, the present invention provides a health functional food composition for improving cognitive function or memory ability using a mixed fermented extract of red ginseng, raw paper, morel, choseokjam and bokryeong as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for preventing or treating degenerative cranial nerve disease and depression containing a mixed fermented product of red ginseng, raw ginseng, morel, choseokjam and bokryeong as an active ingredient.

The mixed fermented extract of red ginseng, Wonji, Morel, Choseokjam and Bokryeong of the present invention has an excellent effect of inhibiting the activity of acetylcholine degrading enzyme, which inhibits neurotransmission by destroying acetylcholine, and scopolamine-induced memory impairment. Since the mouse has excellent recovery ability for memory and cognitive ability, it is highly likely to be usefully used for the prevention or treatment of degenerative cranial nerve diseases such as Alzheimer's dementia. In addition, it can be used as a health functional food composition for improving or enhancing the reduced memory, learning ability and cognitive function.

1 is a result of confirming the cell viability after processing various samples in SH-SY5Y cells, a human neuroblastoma cell line. con is the untreated control group, scop is the group treated with scopolamine to induce memory impairment, and S1 is the group treated with red ginseng powder; S2 is Lactobacillus plantarum ( Lactobacillus plantarum ) WIKIM 18 is a group treated with a mixed fermented product of red ginseng, Wonji, Morel, Choseokjam and Bokryeong prepared using the strain; S3 is Lactobacillus buchneri ( Lactobacillus buchneri ) is a group treated with a mixed fermented product of red ginseng, Wonji, Morel, Choseokjam and Bokryeong prepared using a strain; S4 is Lactobacillus rhamnosus ( Lactobacillus rharmnosus ) Red ginseng prepared using a strain, Wonji, Morel, Choseokjam, and a group treated with a mixed fermented product of Bokryeong; S5 is Lactobacillus plantarum WIKIM 18, Lactobacillus bukneri and Lactobacillus rhamnosus strains prepared using a combination of red ginseng, raw paper, Morel, Choseokjam and bokryeong means a group treated with a mixed fermented product. *** means that the cell proliferation effect is statistically significant compared to the group treated with scopolamine, and the p value is less than 0.001.
Figure 2 is the result of confirming the activity inhibitory effect of acetylcholine degrading enzyme (acetylcholnesterase) after treating various samples to SH-SY5Y cells. *** means that the inhibitory effect of acetylcholinease activity is statistically significant compared to the group treated with scopolamine, and the p value is less than 0.001.
Figure 3 shows the movement of a mouse with impaired short-term memory by oral administration of various samples to a mouse model twice a day for 7 days, and treatment with scopolamine on the 14th day after learning the water maze for 5 days. is the result of checking
4 shows a mouse model with various samples orally administered twice a day for 7 days, and treated with scopolamine on the 14th day after learning the water maze for 5 days. The latency time for mice with impaired short-term memory to find the platform. ) is the measurement result. *** means that the recovery ability of short-term memory impairment is statistically significant compared to the group treated with scopolamine, and the p value is less than 0.001.
Figure 5 is a movement movement of mice with impaired long-term memory by oral administration of various samples to a mouse model twice a day for 7 days, and scopolamine treatment on the 21st day after learning the water maze for 5 days is the result of checking
6 is a mouse model by oral administration of various samples twice a day for 7 days, and after learning the water maze for 5 days, treated with scopolamine on the 21st day to measure the length of time the mouse with impaired long-term memory stays to find the platform. It is the result. *** means that the recovery ability of long-term memory loss is statistically significant compared to the group treated with scopolamine, and the p value is less than 0.001.

The terms or words used in the present specification and claims are not to be construed as limited in their ordinary or dictionary meanings, and that the inventor may appropriately define the concepts of the terms in order to best describe his invention. Based on the principle, it should be interpreted as meaning and concept consistent with the technical idea of the present invention.

Therefore, the matters described in the embodiments, reference examples and drawings described in this specification are only the most preferred examples of the present invention and do not represent all of the technical spirit of the present invention, so they can be substituted at the time of the present application. It should be understood that various equivalents and modifications may be made.

In order to achieve the object of the present invention, the present invention provides a health functional food composition for improving cognitive function or memory ability containing a mixed fermented product of red ginseng, raw paper, morel, choseokjam and bokryeong as an active ingredient.

In the health functional food composition of the present invention, the mixture of red ginseng, raw paper, morel, choseokjam and bokryeong may consist of a weight ratio of 1 to 10:1 to 10:1 to 10:1 to 10, preferably 1 It may be composed of a weight ratio of ~9:1 to 5:1 to 4:1 to 4, and more preferably, may be composed of a weight ratio of 6:1:1:1:1, but is not limited thereto.

In addition, the fermented product is Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus buchneri ( Lactobacillus buchneri ) and Lactobacillus rharmnosus ( Lactobacillus rharmnosus ) is prepared by inoculating a mixture of red ginseng, raw paper, morel, choseokjam and bokryeong It may be one, preferably Lactobacillus plantarum, Lactobacillus bukneri and Lactobacillus rhamnosus in a weight ratio of 1 to 10:1 to 10:1 to 10 It may be prepared by inoculating a mixture of 10 , More preferably, Lactobacillus plan tarum, Lactobacillus bukneri and Lactobacillus rhamnosus may be prepared by inoculating a mixture in a weight ratio of 4:3:3, but is not limited thereto.

In addition, the fermented product may further include fruit juice and honey in addition to the active ingredient, but is not limited thereto. The fruit liquid may be prepared by squeezing and concentrating citron, lemon and tangerine, and noni, resveratrol, and the like may also be used.

The health functional food composition for improving cognitive function and depression or memory ability of the present invention may be prepared in any one formulation selected from powder, granule, pill, tablet, capsule, candy, syrup and beverage, but is not limited thereto.

The health functional food composition is not particularly limited as long as it can be ingested to improve depression and cognitive function or memory ability.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The active ingredient may be used appropriately depending on the purpose of its use (prevention or improvement). In general, in the production of food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There is no particular limitation on the type of the health functional food. Examples of foods to which the health functional food composition can be added include sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, dairy products including ice cream, various soups, beverages, alcoholic beverages and vitamin complexes etc., and includes all health foods in the ordinary sense.

In addition, the health functional food composition of the present invention may be prepared as a food, particularly a functional food. The functional food of the present invention includes ingredients commonly added during food production, for example, proteins, carbohydrates, fats, nutrients and seasonings. For example, when manufactured as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates include monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrin, cyclodextrin, etc.), or sugar alcohols ( For example, xylitol, sorbitol, erythritol, etc.) is preferable. As the flavoring agent, natural flavoring agents (eg, taumatine, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid It may further contain a carbonation agent and the like used in beverages. The ratio of these added ingredients is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of degenerative cranial nerve disease containing a mixed fermented product of red ginseng, raw ginseng, morel, choseokjam and bokryeong as an active ingredient.

In the pharmaceutical composition for preventing or treating degenerative cranial nerve disease of the present invention, a disease in which degenerative changes are induced in nerve cells of the central nervous system due to deterioration of cognitive function or memory ability is a degenerative cranial nerve disease, and a representative degenerative cranial nerve disease includes Alzheimer's dementia (Alzheimer's disease), progressive supranuclear palsy, striatonigral degeneration, Huntington's disease, amyotrophic lateral sclerosis (ALS), cortico-basal nuclear degeneration -basal ganlionic degeneration, diffuse Lewy body disease, Parkinson-ALS-dementia complex of Guam, or Pick's disease.

The pharmaceutical composition of the present invention may further include an appropriate carrier, excipient or diluent commonly used in the preparation of pharmaceutical compositions.

The pharmaceutical dosage form of the composition according to the present invention may be used alone or in combination with other pharmaceutically active compounds, as well as in any suitable group.

The pharmaceutical composition according to the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, or sterile injection solutions according to conventional methods, respectively. can Carriers, excipients and diluents that may be included in the pharmaceutical composition of the present invention include lactose, dextrose sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, and a mixture of methylhydroxybenzo. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations include at least one excipient in the pharmaceutical composition, for example, starch, calcium carbonate, sucrose or lactose, gelatin, etc. is prepared by mixing In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral use include suspensions, internal solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, suspensions, emulsions, freeze-dried formulations, and preservatives etc. may be included. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, Witabsol, Macrogol, Tween 61, cacao butter, laugin oil, glycerogelatin, and the like can be used.

A preferred dosage of the composition of the present invention may vary depending on the patient's condition and weight, the degree of disease, drug form, administration route and period, and may be appropriately selected by those skilled in the art. However, for a desirable effect, the mixed fermented product of red ginseng, raw ginseng, morel, choseokjam and bokryeong of the present invention is administered at 0.1 to 100 mg/kg, preferably 10 to 100 mg/kg, per day. good. Administration may be administered once a day, or may be administered in several divided doses. The above dosage does not limit the scope of the present invention in any way.

The composition of the present invention may be administered to mammals such as rats, mice, livestock, and humans by various routes. Any mode of administration can be envisaged, for example, by oral, rectal or intravenous, intramuscular, subcutaneous injection.

Hereinafter, an embodiment of the present invention will be described in detail. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following examples.

Preparation Example 1. Preparation of mixed fermented product of red ginseng, raw paper, morel, choseokjam and bokryeong

Purified water was added to a mixture of powdered Korean red ginseng (from Korea, dried 4 to 6 years old), raw paper, morel, choseokjam, and bokryeong in a weight ratio of 6:1:1:1:1, followed by high-pressure sterilization.

Lactobacillus plan tarum wikim 18 as a fermentation strain (Lactobacillus plantarum WIKIM 18, KFCC 11588P, World Kimchi Research Institute), Lactobacillus Buk Neri (Lactobacillus buchneri, KCCM 40982, ATCC 4005, DSM 20057) and Lactobacillus ramno suspension (Lactobacillus rharmnosus, KCCM 32405, ATCC 7469, DSM 20021) strains were distributed from the World Institute of Kimchi and the Korea Microorganism Conservation Center. After culturing each in MRS broth and centrifuging, the supernatant was removed and the pellet was freeze-dried.

After mixing the three types of fermented strain powder in a weight ratio of 4:3:3, 1 part by weight was inoculated with respect to 100 parts by weight of the sterilized mixture of red ginseng, raw paper, morel, choseokjam and bokryeong, and cultured at 30°C for 2 days. did. Finally, a mixed fermented product of red ginseng, Wonji, Morel, Choseokjam and Bokryeong was obtained and lyophilized to powder. As a comparative control, each sample was prepared as shown in Table 1 below, and each fermented product (S2 to S4) was prepared by using only three fermentation strains as a raw material, and lyophilized to powder. .

To 30 mg of the freeze-dried sample powder (hereinafter referred to as 'S1, S2, S3, S4, or S5'), 1 ml of DMSO (dimethyl sulfoxide) was added to each and sufficiently dissolved. Each sample dissolved in DMSO was centrifuged to obtain a supernatant, filtered through a 0.25 μm filter, and then a stock solution (30 mg/ml) was prepared and used in the following examples.

Example 1. Measurement of cell viability by MTT assay

For the samples prepared in Preparation Example 1, MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-2Htetrazolium bromide) analysis on nerve cells according to scopolamine treatment The protective effect was confirmed. Various samples prepared in Preparation Example 1 were treated in SH-SY5Y cells, a human neuroblastoma cell line, treated with scopolamine, which induces cranial nerve disease. As a result, while the cell viability decreased due to the scopolamine treatment as shown in FIG. 1, when other samples except for sample S4 of the present invention were treated, the cell viability was similar to that of the control (Con), and in particular, lacto Bacillus plantarum WIKIM 18, Lactobacillus bukneri and a mixed fermented product (S5) of red ginseng, raw paper, Morel, Choseokjam and Bokryeong prepared by inoculating a combination of Lactobacillus bukneri and Lactobacillus rhamnosus strains are the above three strains alone. It was confirmed that the neuroprotective effect was excellent from the result of remarkably increasing the cell viability compared to the mixed fermented products (S2 to S4) of red ginseng, raw paper, morel, choseokjam and bokryeong. (Figure 1. Measurement result of cell viability by MTT analysis)

Example 2. Measurement of Antioxidant Efficacy by DPPH Experiment

Living things, including humans, obtain energy through respiration and convert 2% of absorbed oxygen into free radical free radicals during metabolism. In the living body, antioxidant enzymes (substances) exist as a defense mechanism against these free radicals, and a mechanism for self-protection works.

By using the free radical substance DPPH (2.2-diphenyl-1-picrylhydrazyl), the degree of radical reduction can be measured with a spectrophotometer, and the antioxidant efficacy can be determined by indirectly measuring the antioxidant activity of the material.

Based on fermented red ginseng extract and 6:1:1:1:1 solution of Wonji, Bokryeong, Morel, and Choseokjam, each concentration was prepared. A DPPH solution (0.5 mM, 0.1972 g/L) was prepared, and 1:1 mixed with ethanol was used. Mix 600 µL of sample and 0.5 mM,600 µL of DPPH. After reaction in the dark for 20-30 minutes, dispense 200 μL to 96 well plate. Absorbance was measured at 517 nm, and a comparative graph of sample concentration and absorbance was obtained.

Fig. 1 shows the results of the DPPH antioxidant experiment obtained for a solution in which the fermented red ginseng extract and Wonji, Bokryeong, Morel, and Choseokjam were mixed in a ratio of 6:1:1:1:1.

The value of t1 in FIG. 1 was obtained as 20.0, which is usually _{equivalent to IC 50} , which is interpreted as having a large antioxidant effect.

Example 3. Measurement of the inhibitory effect on the activity of acetylcholinesterase

Acetylcholinesterase is known as an enzyme that inhibits neurotransmission by destroying acetylcholine. Therefore, after processing the various samples prepared in Preparation Example 1 in SH-SY5Y cells, a human neuroblastoma cell line treated with scopolamine, which induces cranial nerve disease, acetylcholine degradation using an ELISA kit (CUSABIO) The inhibitory effect on the activity of the enzyme was confirmed. As a result, as shown in FIG. 2, the production of acetylcholinease was rapidly increased due to scopolamine treatment, but when the samples of the present invention were treated, the production of acetylcholinease was reduced, especially Lactobacillus plan. The mixed fermented product (S5) of red ginseng, Wonji, Morel, Choseokjam and Bokryeong prepared by inoculating a combination of Tarum WIKIM 18, Lactobacillus bukneri, and Lactobacillus rhamnosus strains was obtained by using the above three strains alone. It was confirmed that the production of acetylcholinease was significantly reduced compared to the mixed fermented products (S2 to S4) of the prepared red ginseng, raw paper, morel, choseokjam and bokryeong.

Example 4. Spatial memory recovery effect through the Morris water maze test

The animals used in this experiment were 10-week-old BLAB/C male mice with an average body weight of 26 g, and three animals were placed in one breeding case, and constant temperature (23±2° C.) and constant humidity (50-55%) were maintained, They were bred in the same laboratory environment in which the day and night were alternated at 12-hour intervals, and feed and water were provided so that they could ingest them autonomously. After allowing these mice to undergo an environmental adaptation period for one week, each sample was orally administered at 100 mg/kg twice a day, and the control group (con) was orally administered with the same amount of distilled water. After 5 days of underwater maze learning, the platform was removed and the degree of spatial memory was measured according to how accurately the mice remembered the platform location. Short-term memory was measured on the 14th day after learning the underwater maze, and long-term memory on the 21st day. did. 30 minutes before the memory ability measurement, scopolamine (SCOP, scopolamine) 2mg/kg was administered by IP (intraperitoneal). As a result, as shown in FIGS. 3 and 4, in the group treated with scopolamine alone, compared to the control group (Con) that was not treated with anything, the mouse not only attempted numerous direction changes to find the position of the platform, but also caused memory impairment. The length of the copper wire was complicated and the retention time (54 seconds) was increased. On the other hand, in the treatment group of the samples (S1 to S4) of the present invention, the number of direction changes and the length of the copper wire were somewhat reduced, and it was found that the retention time was also reduced. Furthermore, among the samples of the present invention, Lactobacillus plantarum WIKIM 18, Lactobacillus bukneri and a mixed fermented product of red ginseng, raw paper, Morel, Choseokjam and Bokryeong prepared using a combination of Lactobacillus bukneri and Lactobacillus rhamnosus strain (S5) In the case of oral administration, it was confirmed that the short-term memory ability damaged by scopolamine was remarkably recovered compared to the case where each of the three strains was used alone (S2 to S4).

Similarly, in the long-term memory analysis results, when the mixed fermented product (S5) of red ginseng, Wonji, Morel, Choseokjam and Bokryeong of the present invention was orally administered among the samples, long-term memory ability damaged due to scopolamine was remarkably recovered. was known to have been

The above description is merely illustrative of the technical spirit of the present invention, and various modifications and variations will be possible without departing from the essential characteristics of the present invention by those skilled in the art to which the present invention pertains.

Therefore, the embodiments disclosed in the present invention are not intended to limit the technical spirit of the present invention, but to explain, and the scope of the technical spirit of the present invention is not limited by these embodiments. The protection scope of the present invention should be construed by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (6)

A health functional food composition for improving cognitive function or memory ability, comprising a mixed fermented product of red ginseng, raw paper, morel, choseokjam and bokryeong as active ingredients
According to claim 1,
A health functional food composition for improving cognitive function or memory ability, characterized in that the red ginseng, raw paper, morel, choseokjam and bokryeong are mixed in a weight ratio of 1-10:1-10:1-10:1-10.
According to claim 1,
The fermented product is Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus buchneri ( Lactobacillus buchneri ) and Lactobacillus rharmnosus to a mixture of red ginseng, raw paper, morel, choseokjam and bokryeong prepared by inoculating a combination of Lactobacillus rharmnosus A health functional food composition for improving cognitive function or memory ability, characterized in that.
According to claim 1,
The composition is a health functional food composition for improving cognitive function or memory, characterized in that it is prepared in any one formulation selected from gel sticks, powders, granules, pills, tablets, capsules, candy, syrup and beverages.
A pharmaceutical composition for the prevention or treatment of depression and degenerative cranial nerve diseases, containing a mixed fermented product of red ginseng, raw ginseng, morel, choseokjam and bokryeong as active ingredients.
6. The method of claim 5,
In addition to the active ingredient, a pharmaceutical composition for preventing or treating depression and degenerative cranial nerve disease, characterized in that it further comprises a pharmaceutically acceptable carrier, excipient or diluent.

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