KR20210068802A - Magnetic Drive Anaerobic Fermentor - Google Patents

Abstract

The present invention discloses a magnetic drive anaerobic fermenter consisting of a fermentation tank (110), an anaerobic module (120), a magnetic drive module (130), a heater module (140), and a control module (150). The fermentation tank and the magnetic drive module are mutually removable, and anaerobic microorganisms are cultivated while maintaining the watertightness of the fermentation tank so as not to be contaminated from the external environment. By precisely creating an oxygen-free environment in the fermentation tank, microorganisms can be cultivated.

Description

Magnetic Drive Anaerobic Fermentor

The present invention relates to a magnetic drive anaerobic fermenter, and more particularly, it is possible to configure the fermenter and the magnetic drive module to be mutually detachable, and to culture the anaerobic microorganisms while maintaining the watertightness of the fermenter so as not to be contaminated from the external environment. It relates to magnetic drive anaerobic fermenters.

In general, a sampler is used to culture microorganisms through a fermentor capable of various microbial anaerobic biological processes and collect a culture solution sample.

On the other hand, as a related prior art, Korean Patent Publication No. 0913775 is disclosed. A conventional biological incubator includes a reaction vessel for culturing, a jacket for controlling the temperature of the reaction vessel, and a method for stirring the liquid in the reaction vessel. It consists of a culture tank including a stirrer, a control valve connected to the reaction vessel or the jacket, and a culture condition control unit for controlling the temperature of the reaction vessel, the motor of the agitator, and the control valve, so as to facilitate the control of the biological incubator.

However, since the agitator is driven through the mechanical connection of the motor, perfect watertightness or airtightness is difficult, so the possibility of contamination by external air exists, and it is impossible to apply a culture tank of various capacities.

Accordingly, a fermenter technology capable of minimizing the possibility of contamination by watertightening the stirrer and applying a culture tank of various capacities is required.

Korean Patent Publication No. 0913775 (Biological Incubator, 2009.08.26)

The technical problem to be achieved by the spirit of the present invention is to configure the fermenter and the magnetic drive module to be mutually detachable, and to culture the anaerobic microorganisms while maintaining the watertightness of the fermenter so as not to be contaminated from the external environment, magnetic drive To provide an anaerobic fermenter.

In order to achieve the above object, the present invention is composed of a cylindrical transparent container having an upper end covered with an upper plate and a middle plate and a lower end covered with a lower plate, and a support for mutually supporting the middle plate and the lower plate, a fermenter forming a space for performing anaerobic fermentation; A plurality of gas supply ports formed on the upper end of the upper plate of the fermenter and supplying various gases to the inside of the transparent container to provide a culture environment, a nitrogen sparger for supplying nitrogen, and an air supply for supplying compressed air A port, an inoculum for supplying microorganisms, a sampling port for supplying the cultured microorganism culture solution from the transparent container to the sampler, and a feed pump for pumping the corresponding gas to the gas supply port and the nitrogen sparger, respectively, anaerobic module; A rotating shaft formed on the central axis of the transparent container, a turbine impeller formed radially symmetrically to the rotating shaft, an upper magnetic drive coupled to the end of the rotating shaft at the upper end of the lower plate, and the upper magnetic at the lower end of the lower plate a magnetic drive module comprising: a lower magnetic drive spaced apart from the drive; and a servomotor rotating the lower magnetic drive; a heater coupled to a lower end of the lower plate and a heater module in which a heater block and a Teflon heat-resistant layer are laminated; and a control module for controlling the fermenter, the anaerobic module, the magnetic drive module, and the heater module, respectively, performing carbon dioxide and nitrogen substitution, and controlling nitrogen and oxygen concentrations; including, the fermenter and the magnetic drive The module is mutually detachable and provides a magnetic drive anaerobic fermenter for culturing anaerobic microorganisms while maintaining the watertightness of the fermenter.

In addition, it may further include a plurality of baffles formed radially in the height direction on the inner wall of the transparent container and the baffles for coupling the baffles to each other.

In addition, it may further include a foam sensor for detecting bubbles generated when the culture solution is stirred by the fermenter, a gas sensor for detecting each gas, a DO sensor for detecting DO, and a pH sensor for detecting pH have.

In addition, the turbine impeller, a form breaker blade fixed to the upper end of the rotary shaft, a turbine impeller blade fixed to the middle to agitate the culture medium, and a magnetic turbine impeller blade fixed radially to the upper magnetic drive. .

In addition, a spindle-shaped shaft bearing in contact with the bearing port coupled to the lower plate may be interposed at one end of the rotating shaft.

In addition, it may further include a plunger coupled to the lower end of one side of the heater block for detecting the seating of the fermenter to the magnetic drive module, and a microswitch for driving the heater module by the plunger.

In addition, the control module may include a communication module to enable remote control through an external device.

In addition, it may further include a holder for stacking and storing reagent bottles for culture.

According to the present invention, the fermenter and the magnetic drive module are configured to be mutually detachable, the anaerobic microorganisms are cultivated while the fermenter is kept watertight so as not to be contaminated from the external environment, and an oxygen-free environment is precisely created in the fermenter. This allows for culturing microorganisms, facilitates batch/fed-batch/continuous fermentation, establishes various culture environments, enables remote control, and has the effect of applying fermenters of various capacities.

1 is a perspective view of a magnetic drive anaerobic fermenter according to an embodiment of the present invention.
Figure 2 shows a side view of the magnetic drive anaerobic fermenter of Figure 1;
3 and 4 are exploded perspective views of the magnetic drive anaerobic fermenter of FIG. 1 .
5 to 7 are views showing the separation of the fermenter and the magnetic drive module of the magnetic drive anaerobic fermenter of FIG. 1 .
FIG. 8 shows a cross-sectional structure of the main configuration of FIG. 7 .
9 is an exploded perspective view of the magnetic drive module of the magnetic drive anaerobic fermenter of FIG. 1 .

Hereinafter, embodiments of the present invention having the above-described characteristics will be described in more detail with reference to the accompanying drawings.

1 to 9, the fermenter 110, the anaerobic module 120, the magnetic drive module 130, the heater module 140 constituting the magnetic drive anaerobic fermenter according to the embodiment of the present invention, and , the control module 150 will be described in detail as follows.

First, the fermenter 110, as shown in FIGS. 5 and 6, the upper end is covered with the upper plate 111 and the middle plate 112, and the lower end is a cylindrical transparent container covered with the lower plate 113 ( vessel) 114, and a support 115 for mutually supporting the middle plate 112 and the lower plate 113. The magnetic drive module ( 130) and formed to be separated.

Here, the transparent container 114 may be formed to be watertightly coupled by the middle plate 112 and the lower plate 113 and the ring-shaped silicon packings 116a and 116b, respectively.

In addition, a pair of handles 117 are formed on both sides of the upper plate 111 of the fermenter 110, so that the fermenter 110 of various sizes of 1.5 ml to 15 L can be easily attached to and detached from the magnetic drive module 130 at the top. can be configurable.

In addition, as shown in FIG. 6, a plurality of baffles 118a radially formed on the inner wall of the transparent container 114 in the height direction and a baffle rib for coupling the baffles 118a to each other (baffle rib) ( 118b) can be further included to increase the stirring effect of the culture medium.

Next, the anaerobic module 120, as shown in FIG. 7, is formed on the upper end of the upper plate 111 of the fermenter 110, to the inside of the transparent container 114, to provide an anaerobic microorganism culture environment, oxygen And a plurality of gas supply ports 121 for supplying or removing various gases of carbon dioxide, a nitrogen sparger 122 for supplying nitrogen, and an air supply port for supplying compressed air by a compressor (not shown) (123), and an inoculum (inoculum) 124 for supplying microorganisms, and a sampling port (sampling port) for supplying the cultured microbial culture solution to a sampler (not shown) for collecting a culture solution sample from the transparent container 114 . ), and a feed pump 126 (see FIG. 3 ) for pumping the corresponding gas to the gas supply port 121 and the nitrogen sparger 122, respectively, to replace carbon dioxide and nitrogen through valve control. and control the nitrogen concentration and oxygen concentration.

Next, the magnetic drive module 130, as shown in Figs. 4 and 6, the longitudinal rotation shaft 131 formed on the central axis of the transparent container 114, and radially symmetrical to the rotation shaft 131 The formed turbine impeller, the upper magnetic drive 133 coupled to the end of the rotary shaft 131 from the upper end of the lower plate 113, and the upper magnetic drive 133 from the lower end of the lower plate 113 It is composed of a lower magnetic drive 134 spaced apart and a servo motor 135 for rotationally driving the lower magnetic drive 134, without direct contact, the upper magnetic drive 133 and the lower magnetic drive 134 Stirring can be performed by rotating the turbine impeller by the attractive force of

Here, the turbine impeller is a form breaker blade (132a) for removing bubbles formed on the surface of the culture medium fixed to the top of the rotary shaft 131, and a turbine impeller blade, which is a main blade fixed to the middle and agitating the culture medium. (132b) and the magnetic turbine impeller blade (132c) radially fixed to the upper magnetic drive 133 is spaced apart and configured, it is possible to agitate the culture medium while removing unnecessary bubbles generated during fermentation.

On the other hand, as shown in enlarged view in FIG. 9 , a spindle-shaped shaft bearing 137 in contact with the bearing port 136 coupled to the lower plate 113 is interposed at one end of the rotating shaft 131, so that the magnetic drive rotation can be facilitated.

Accordingly, the fermenter 110 and the magnetic drive module 130 are configured to be mutually detachable, and the anaerobic microorganisms can be cultured while the fermenter 110 is kept watertight so as not to be contaminated from the external environment.

Next, the heater module 140, as shown in FIGS. 4 and 9, a heater 141 coupled to the lower end of the lower plate 113, a heater block 142 and a Teflon heat-resistant layer 143 are laminated. However, the heater 141 is composed of a mica heater (mica), so as to maintain the temperature for the optimal culture environment of the fermenter 110.

On the other hand, a plunger 144 coupled to the lower end of one side of the heater block 142 and sensing the seating of the lower plate 113 of the fermenter 110 to the magnetic drive module 130 and the plunger 144 by By further including a microswitch 145 for driving the heater module 140, the magnetic drive module 130 or the heater module 140 may be driven only when the fermenter 110 is seated.

Next, the control module 150 controls the fermenter 110, the anaerobic module 120, the magnetic drive module 130, and the heater module 140, respectively, controls the stirring speed, performs carbon dioxide and nitrogen substitution, , nitrogen concentration and oxygen concentration are controlled.

In addition, by the control module 150, the anaerobic microorganism fermentation process data can be stored externally through the USB port 171 (refer to FIG. 1) and can be converted into a database, including a communication module (not shown), an external device It is also possible to control the fermentation process through remote control through an external controller (not shown).

On the other hand, as shown in FIG. 7 , a foam sensor 161 for detecting bubbles generated when the culture solution is stirred by the fermenter 110 and a gas sensor 162 for detecting a gas, for example, a carbon dioxide concentration or a nitrogen concentration, respectively. And, by further including a DO sensor 163 for detecting DO (dissolved oxygen), and a pH sensor 164 for detecting pH, it is possible to precisely control the culture process or fermentation process.

In addition, as shown in FIGS. 1 and 2, it further includes a holder 172 for stacking and storing reagent bottles necessary for culturing anaerobic microorganisms adjacent to each other, thereby providing convenience to the experimenter.

Therefore, by the configuration of the magnetic drive anaerobic fermenter as described above, the fermenter and the magnetic drive module are configured to be mutually detachable, and the anaerobic microorganisms are cultured while maintaining the watertightness of the fermenter so as not to be contaminated from the external environment, Accurately create an oxygen-free environment in the fermenter to culture microorganisms, facilitate batch/fed-batch/continuous fermentation, establish a variety of culture environments, perform remote control, and apply fermenters of various capacities can do.

The embodiments described in this specification and the configurations shown in the drawings are only the most preferred embodiment of the present invention, and do not represent all the technical spirit of the present invention, so various equivalents that can be substituted for them at the time of the present application It should be understood that there may be water and variations.

110: fermenter 111: top plate
112: middle plate 113: bottom plate
114: transparent container 115: support
116a,b: silicone packing 117: handle
118a: Bestlive 118b: Bestlive
120: anaerobic module 121: gas supply port
122: nitrogen sparger 123: air supply port
124: inoculum 125: sampling port
126: feed pump 130: magnetic drive module
131: rotating shaft 132a: foam breaker blade
132b: turbine impeller blade 132c: magnetic turbine impeller blade
133: upper magnetic drive 134: lower magnetic drive
135: servo motor 136: bearing port
137: shaft bearing 140: heater module
141: heater 142: heater block
143: Teflon heat-resistant layer 144: plunger
145: micro switch 150: control module
161: form sensor 162: gas detection sensor
163: DO sensor 164: pH sensor
171: USB port 172: cradle

Claims (8)

A fermenter comprising: a cylindrical transparent container having an upper end covered with an upper plate and a middle plate and a lower end covered with a lower plate, and a support for mutually supporting the middle plate and the lower plate to form a space for performing anaerobic fermentation;
A plurality of gas supply ports formed on the upper end of the upper plate of the fermenter and supplying various gases to the inside of the transparent container to provide a culture environment, a nitrogen sparger for supplying nitrogen, and an air supply for supplying compressed air A port, an inoculum for supplying microorganisms, a sampling port for supplying the cultured microorganism culture solution from the transparent container to the sampler, and a feed pump for pumping the corresponding gas to the gas supply port and the nitrogen sparger, respectively, anaerobic module;
A rotating shaft formed on the central axis of the transparent container, a turbine impeller formed radially symmetrically to the rotating shaft, an upper magnetic drive coupled to the end of the rotating shaft at the upper end of the lower plate, and the upper magnetic at the lower end of the lower plate a magnetic drive module comprising: a lower magnetic drive spaced apart from the drive; and a servomotor for rotationally driving the lower magnetic drive;
a heater coupled to a lower end of the lower plate, a heater block and a heater module in which a Teflon heat-resistant layer is laminated; and
A control module for controlling the fermenter, the anaerobic module, the magnetic drive module, and the heater module, respectively, performing carbon dioxide and nitrogen substitution, and controlling nitrogen and oxygen concentrations; including, the fermenter and the magnetic drive module Is mutually detachable, magnetic drive anaerobic fermenter for culturing anaerobic microorganisms while maintaining the watertightness of the fermenter.
According to claim 1,
Magnetic drive anaerobic fermenter, characterized in that it further comprises a plurality of baffles radially formed on the inner wall of the transparent container in the height direction and the baffles for mutually coupling the baffles.
According to claim 1,
It further comprises a foam sensor for detecting bubbles generated when the culture solution is stirred by the fermenter, a gas sensor for detecting each gas, a DO sensor for detecting DO, and a pH sensor for detecting pH which is a magnetic drive anaerobic fermenter.
4. The method of claim 3,
The turbine impeller is a form breaker blade fixed to the upper end of the rotating shaft, a turbine impeller blade fixed to the middle stage to stir the culture solution, and a magnetic turbine impeller blade fixed radially to the upper magnetic drive. , magnetic drive anaerobic fermenter.
According to claim 1,
A magnetic drive anaerobic fermenter, characterized in that a spindle-shaped shaft bearing in contact with the bearing port coupled to the lower plate is interposed at one end of the rotating shaft.
According to claim 1,
Magnetic drive anaerobic fermenter further comprising: a plunger coupled to the lower end of one side of the heater block to detect the seating of the fermenter into the magnetic drive module; and a microswitch for driving the heater module by the plunger .
According to claim 1,
The control module, including a communication module, magnetic drive anaerobic fermenter, characterized in that to enable remote control through an external device.
According to claim 1,
Magnetic drive anaerobic fermenter, characterized in that it further comprises a cradle for stacking and storing reagent bottles for culture.

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