KR20210065637A - Composition for improving hangover cure and alcoholic liver injury comprising pericarp of Areca catechu L and and Ledebouriella seseloides - Google Patents

Abstract

The present invention relates to a composition for easing a hangover and alleviating alcoholic liver injury comprising a mixed extract of the pericarp from Areca catechu and the root of Ledebouriella seseloides as active ingredients. The composition comprising the mixed extract of the pericarp from Areca catechu and the root of Ledebouriella seseloides of the present invention has excellent antioxidant effects, lowers a blood alcohol concentration as confirmed in a liver damage induction model by acute alcohol administration, enhances the activity of acetaldehyde dehydrogenase, which breaks down acetaldehyde, a toxic substance produced during alcohol metabolism and reduces lipid peroxidation caused by the alcohol administration.

Description

Composition for improving hangover cure and alcoholic liver injury comprising pericarp of Areca catechu L and and Ledebouriella seseloides

The present invention relates to a composition for relieving a hangover and improving alcoholic liver damage using a mixed extract of daabbokki and windbreak.

The liver is the organ in which metabolism in the body occurs most actively among human body organs. Although the liver is classified as a digestive system, it is actually involved in almost everything that happens in our body, including carbohydrate metabolism, amino acid and protein metabolism, fat metabolism, bile acid and bilirubin metabolism, vitamin and mineral metabolism, hormone metabolism, detoxification and bactericidal action.

The liver is located between the digestive system and the systemic circulatory system in the human body, and performs the function of defending the whole body from external substances introduced from the outside. Since ex vivo substances that enter the body pass through the liver once, the liver has a higher risk of being exposed to many toxic substances in addition to nutrients than other organs, so the probability of damage is very high. However, the liver is an organ with excellent regenerative ability, and if there is a slight damage, it recovers sufficiently to normal, but if the damage continues, a part of the liver tissue is completely destroyed and the liver function is reduced. do. When such liver damage becomes chronic, it progresses to liver fibrosis, cirrhosis, or liver cancer regardless of the cause.

On the other hand, there are various causes of liver damage, such as chronic stress-induced fatigue, excessive consumption of food or alcohol containing fat, viral infection, harmful substances such as various drugs, and malnutrition.

It is excellent in the therapeutic effect on such liver damage, and there is a need to develop a new therapeutic agent for liver disease using a natural product that does not have side effects even when administered in large quantities or for a long period of time.

The betel rind is the pericarp of Areca catecthu L, a tree belonging to the palm family, and the seeds of betel nut contain arecoline, arecaine, and guvacin, which are alkaloids, and myristine of fatty acids. It is known to contain ingredients such as myristin acid and palmitin acid (by Bo Bo-seop and Shin Min-gyo, Dohaehyangyak (Cerbal Medicine) Metabolism , Youngrimsa, pp 272-274, 1998). It has a slightly peculiar smell, and the taste is spicy and slightly astringent, and the nature is slightly warm. Daebokpi is used for lowering the qi and is used for chest and stomach stuffiness, edema, and constipation, and it is reported that betel nut has a parasite extermination effect as a pharmacological action.

Windbreak is the windbreak of Ledebouriella seseloides WOLF. = Siler divaricatum (TURCZ.) BENTH, a three-year-old plant belonging to the family Buttercup, and the root of the flora and fauna, and contains essential oils, mannitol, bitter glycosides, etc. It is known to contain (both Bo-seop and Shin Min-gyo, Dohaehyangyak (herbal medicine) dictionary , Youngrimsa, pp 428, 1998). Windbreak is native to northeastern China, Hebei, and Inner Mongolia, and is cultivated as a medicinal crop. In oriental medicine, the biennial root is used as medicine for colds, headaches, sweating and expectoration. It is distributed in Korea, China, Usuri River, Mongolia, and Siberia.

Korean Patent Registration No. 10-0626724 Korean Patent Publication No. 10-2014-0077483 Korean Patent Publication No. 10-2015-0037204 Korean Patent Publication No. 10-2019-0064558

An object of the present invention is to provide a composition that can effectively improve hangover relieving and alcoholic liver damage by using a mixed extract extracted by mixing daebokki, which is a natural drug, and windbreak.

In order to achieve the above object, the present invention provides a composition for relieving a hangover and improving alcoholic liver damage, which contains a mixed extract of daabbokki and windbreak as an active ingredient.

In the composition, it is preferable that the cover and windbreak are mixed in a weight ratio of 1:1.

In the composition, the extract is preferably extracted using 70% ethanol as an extraction solvent.

In the composition, the mixed extract of the daebokgi and windbreak,

Putting a mixture of robes and windbreaks in 8-12 times (v/w) 70% ethanol and immersing them at 55-65° C. for 20-28 hours; obtaining an extract after immersion; filtering the obtained extract; And it is preferably prepared by a method comprising the step of concentrating and drying the filtered extract under reduced pressure.

The composition containing the mixed extract of periwinkle and fenugreek of the present invention has excellent antioxidant effect, lowers blood alcohol concentration as confirmed in the liver damage induction model by acute alcohol administration, and acetone, a toxic substance produced in the process of alcohol metabolism It increases the activity of acetaldehyde dehydrogenase that decomposes aldehydes and has the effect of reducing lipid peroxidation caused by alcohol administration.

Therefore, the composition of the present invention can be effectively used to relieve hangover and improve alcoholic liver damage.

1 is a result of comparing the effect of a mixed extract on blood alcohol concentration and ALDH activity according to the mixing ratio of daabapki and windbreak in a liver injury induction model by acute alcohol administration.
Figure 2 is a result of comparing the effect of a mixed extract of daabbokki and Bangpung on blood alcohol concentration and ALDH activity according to the extraction solvent in a liver injury induction model by acute alcohol administration.
Figure 3 is a result of comparing the antioxidant effect of the extract of the mixed extract of daabbokki and periwinkle with the single extract of daabbokki and bangpung.
4 is a result of comparing the effect of a mixed extract of daabbokki and bangpung on lipid peroxidation in a liver injury induction model by acute alcohol administration with a single extract of daabbokki and bangpung.

The present invention relates to a composition for relieving a hangover and improving alcoholic liver damage containing a mixed extract of abalone and fenugreek as an active ingredient.

It is preferable to extract by mixing the robes and windbreaks in a weight ratio of 1:2 to 2:1, and it is particularly preferable to extract by mixing them in a weight ratio of 1:1.

It is preferable to use 70% ethanol as an extraction solvent during extraction. As the extraction method, a method commonly used for extraction may be used.

The mixed extract of the periwinkle and windbreak is put in 8-12 times (v/w) 70% ethanol and immersed at 55-65° C. for 20-28 hours, and the extract is obtained after immersion It is more preferable to prepare by filtering the obtained extract and concentrating and drying the filtered extract under reduced pressure.

The step of obtaining the extract by immersion can be repeated 2-3 times, and in the case of repeating, the obtained extract is collected together and then subjected to a filtration process.

Since the composition of the present invention is excellent in the effect of relieving hangover and improving alcoholic liver damage, it can be used in pharmaceutical compositions, health foods, etc. for hangover relieving and improvement of alcoholic liver damage.

The present invention will be described in more detail with reference to the following examples. These examples illustrate the present invention, but the scope of the present invention is not limited thereto.

<Example 1>

100 g of a mixture obtained by mixing robes and windbreaks purchased from Human Hub (http://www.humanherb.co.kr/) in a 1:1 weight ratio in 1,000 ml of 70% ethanol at 60°C for 24 hours immersed.

After immersion, an extract was obtained at room temperature, and 1,000 ml of 70% ethanol was added thereto and extracted twice more to collect the extract.

The collected extract was filtered, and the filtered filtrate was concentrated under reduced pressure to evaporate the solvent using a vacuum rotary evaporator (Nihon Seiko, Japan, VR-205c) and dried to obtain a 70% ethanol extract.

<Example 2>

A 70% ethanol extract was obtained in the same manner as in Example 1, except that 100 g of a mixture obtained by mixing sagebrush and windbreak in a weight ratio of 1:2 was used as a raw material.

<Example 3>

A 70% ethanol extract was obtained in the same manner as in Example 1, except that 100 g of a mixture obtained by mixing robes and windbreaks in a weight ratio of 2:1 was used as a raw material.

<Comparative Example 1>

A 70% ethanol extract was obtained in the same manner as in Example 1, except that 100 g of periwinkle was used as a raw material.

<Comparative Example 2>

A 70% ethanol extract was obtained in the same manner as in Example 1, except that 100 g of windbreak was used as a raw material.

<Comparative Example 3>

A hot water extract was obtained in the same manner as in Example 1, except that hot water was used as the extraction solvent.

<Experimental Example 1>

Effect by mixing ratio of abalone and windbreak in a liver injury induction model by acute alcohol administration

As the experimental animal, a 7-week-old male Sprague-Dawley rat was used.

As an experimental sample, a 1:1 mixed extract (Example 1), a 1:2 mixed extract (Example 2), a biceps skin and a windbreak, a 2:1 mixed extract (Example 3) ), a single extract of periwinkle (Comparative Example 1) and a single extract of windbreak (Comparative Example 2) were dissolved in physiological saline and administered orally at a concentration of 200 mg/kg by 0.5 ml each. The same amount of physiological saline was orally administered to the positive control group (PC).

30 minutes after administration of the experimental sample, 4 g of 50% ethanol per kg of body weight was orally administered to induce liver damage by acute alcohol administration, thereby obtaining a liver injury induction model (in vivo) by acute alcohol administration.

In the liver injury induction model by the acute alcohol administration, ethanol was orally administered, and blood was collected from the abdominal vena cava after 45 minutes, 3 hours, and 8 hours.

The blood was left at room temperature for 30 minutes and then centrifuged (3,000 rpm, 10 minutes) to collect only the serum, and analyze the ethanol in serum using an ethanol assay kit, followed by an acetaldehyde dehydrogenase kit. (acetaldehyde assay kit) was used to analyze ALDH (acetaldehyde dehydrogenase) activity. The results are shown in FIG. 1 .

As shown in FIG. 1, in the liver injury induction model by acute alcohol administration, the ethanol administration group showed a high blood alcohol concentration, and the experimental group administered with a 1:1 mixed extract of daabbokki extract and pericardium alone It was confirmed that the blood alcohol concentration was 60.5% and 105.2% lower, respectively, compared to the experimental group to which the extract was administered. In addition, it was confirmed that the ALDH activity was highest in the experimental group administered with 1:1 mixed extracts of periwinkle and persimmon.

<Experimental Example 2>

Effect of Extraction Solvent in Acute Alcohol Induction Induction Model

Experiments were carried out in the same manner as in Experimental Example 1, except that 70% ethanol extracts of periwinkle and windbreak (Example 1) and hot-water extracts of pericardium and windbreak (Comparative Example 3) were used as experimental samples.

As shown in FIG. 2 showing the results, the extract using 70% ethanol as an extraction solvent in the serum 8 hours after alcohol administration decreased blood alcohol concentration by about 60.5% and ALDH activity increased by about 52.6% compared to the hot water extract. was confirmed to be

<Experimental Example 3>

antioxidant effect

The antioxidant effect of the mixed extract of periwinkle and windbreak of the present invention was confirmed by DPPH analysis, ABTS radical analysis, FRAP analysis, and total phenol content as follows.

As a sample, the mixed extract of the inner peritoneum of Example 1 and the peritoneum extract of Comparative Example 1 and the windbreak extract of Comparative Example 2 were used. Vitamin C (Vit C) was used for control.

(1) DPPH radical scavenging activity (DPPH assay)

The radical scavenging activity for DPPH was measured according to the method of Blois MS (1958). To 160 μl of each sample, 80 μl of 0.2 mM DPPH (1,1-diphenyl-2-picrylhydrazyl) was added and stirred. After stirring, it was allowed to stand for 30 minutes, and then absorbance was measured at 517 nm using a microplate reader.

(2) ABTS radical scavenging activity (ABTS assay)

A 7mM ABTS solution and 2.45mM potassium persulfate (final concentration) were mixed and reacted for 12 hours in a shaking incubator blocked from light at room temperature to generate radicals. After generating radicals, it was diluted with ethanol (100%) so that the absorbance at 415 nm was 0.7±0.02. After mixing 250 μl ABTS solution and 28 μl sample and reacting at room temperature for 5 minutes, absorbance at 415 nm was measured using a spectrophotometer. Trolox was used as a positive control. The measured absorbance was calculated by Equation 1 below.

(3) FRAP assay

This is an analysis method using the principle that iron is oxidized to trivalent in TPTZ (Ferric 2,4,6-tripyridyl-s-triazine) reagent by oxidation reaction and converted into a colored product.

A 1 mM solution (0.278 g FeSO ₄ .7H ₂ O/L) was prepared using ferrous sulphate and used as a standard solution, and the FRAP solution was 300 mM acetate buffer (pH3.6) 200 ml, 10 mM TPTZ solution 20 ㎖, 20mM FeCl ₃ solution 20㎖, was prepared by mixing 24㎖ sterile water was used by maintaining at 37 ℃. After mixing 170 μl of sterile water per well, 7 μl of standard solution or sample solution and 30 μl of FRAP solution in a 96-well plate, absorbance was measured at 593 nm after 4 minutes. The FRAP level was calculated through linear regression analysis of the absorbance value for a standard solution.

(4) Total phenol content

Total phenol content was determined by Folin-Ciocalteu assay. This method uses a mixture of tungstate and molybdate in an alkaline solution (5-10% soluble sodium carbonate). The phenolic compound is oxidized _{to form O 2} ^- and reacts with molybdate to form molybdenum oxide. To form (molybdenum oxide) (MoO ⁴⁺ ), the material thus formed was measured at 750 nm, and the value was expressed as gallic acid equivalents (GAE).

(5) Results

The results of confirming the antioxidant effect are shown in FIG. 3 . As shown in the results of Figure 3, as a result of testing the effect on major factors related to antioxidants, the DPPH radical scavenging activity, ABTS radical scavenging activity, FRAP value, and total phenol content were all mixed extracts of Korean radish and windbreak (1:1). ratio) showed a higher activity than the extracts of Daebokpi and Bangpung alone.

<Experimental Example 4>

Effects on lipid peroxidation in a liver injury induction model by acute alcohol administration

As experimental samples, a 1:1 mixed extract (Example 1), a single extract (Comparative Example 1), and a single extract (Comparative Example 2) of perilla perilla and perilla perforum were 10 µg/ml, 50 µg/ml, 100 µg, respectively. The experiment was conducted in the same manner as in Experimental Example 1 except that it was used at a concentration of μg/ml and 200 μg/ml, and lipid peroxidation in the rat tissue used in the experiment was measured as follows.

After the liver tissue was perfused (perfusion), the liver weight was measured. Ice cold PBS corresponding to 10% of the measured liver tissue weight was added, and the tissue was suspended using a sonicator.

The suspension was centrifuged at 10,000×g for 5 minutes to obtain a supernatant. 50 μl of the supernatant and 500 μl of 0.15M KCl were mixed well, and then 100 μl of _{20mM FeCl 3 was added.} The mixture was incubated at 37° C. for 30 minutes, and 1 ml of the reaction termination solution (0.25N ice-cold HCl + 15% TCA + 0.38% TBA + 0.05% BHT) was added to terminate the reaction.

After incubation at 90° C. for 30 minutes, centrifugation was performed at 10,000×g for 5 minutes. The absorbance at 535 nm was measured for the pink sample obtained after centrifugation using a spectrophotometer.

The lipid peroxidation value was _{calculated as a percentage compared with FeCl 3 ,} and the results are shown in FIG. 4 .

As shown in Figure 4, the lipid peroxidation value of the experimental group administered with the mixed extract of periwinkle and periwinkle was reduced by about 30.8%, 43.1% compared to the experimental group administered with the extract alone and pericardium alone at a concentration of 200 μg/ml. was confirmed to be

Claims (4)

A composition for relieving a hangover and improving alcoholic liver damage, containing a mixed extract of abalone skin and windbreak as an active ingredient. According to claim 1,
The composition, characterized in that the cover and windbreak are mixed in a weight ratio of 1:2 to 2:1. According to claim 1,
The composition is characterized in that the extract is extracted using 70% ethanol as an extraction solvent. According to claim 1,
The mixed extract of the abalone skin and windbreak,
Putting a mixture of robes and windbreaks in 8-12 times (v/w) 70% ethanol and immersing them at 55-65° C. for 20-28 hours;
obtaining an extract after immersion;
filtering the obtained extract; and
A composition, characterized in that it is prepared by a method comprising the step of concentrating and drying the filtered extract under reduced pressure.

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