KR20210059236A - Method of producing the extract of rutin using functional buckwheat buds - Google Patents

Abstract

The present invention relates to a method for managing a rutin extract using functional buckwheat sprouts, which provides an advantage of using, as a functional material for food and bio industries, the residue which is remained after buckwheat seeds are germinated to be cultivated into sprouts and an extraction process is performed for using the sprouts as a material for food and the like. According to an embodiment of the present invention, the method comprises: 1) a hot water treatment step of adding distilled water in an amount equivalent to ten times of the weight of buckwheat sprouts and heating the mixture at a temperature of 80 to 90°C for 11 to 13 hours; 2) an extract acquisition step of using a filter paper to acquire an extract from a buckwheat sprout extract completing the process of the step 1); 3) a buckwheat sprout residue drying step of drying buckwheat sprout residues acquired by drying the buckwheat sprout residues not filtered in the extract acquisition step of the step 2) at a temperature of 45 to 55°C for 23 to 25 hours in a hot air machine; and 4) a following extract acquisition step of adding distilled water ten times of the weight of the dried buckwheat sprout residue acquired in the buckwheat sprout residue drying step of the step 3) and repeating the processes of the hot water treatment step, the extract acquisition step, and the residue drying step.

Description

Manufacturing method of rutin extract using functional buckwheat sprout {METHOD OF PRODUCING THE EXTRACT OF RUTIN USING FUNCTIONAL BUCKWHEAT BUDS}

The content disclosed in the present specification relates to a method for preparing a rutin extract using functional buckwheat sprouts, and in particular, after germinating buckwheat and cultivating it as a sprout, it undergoes an extraction process for use as a material such as food, and the functional substance using the remaining residue. It relates to a method for producing a rutin extract using functional buckwheat sprouts for use as a material in food and bio industries.

Unless otherwise indicated herein, the content described in this section is not prior art to the claims of this application, and inclusion in this section is not admitted to be prior art.

Two types of buckwheat are cultivated, general buckwheat and tatari buckwheat, and in general, many processed foods using general buckwheat are produced.

Such, buckwheat contains rutin, which is a functional substance related to improvement of vascular-related diseases, and tatari buckwheat contains 10 times or more of the content of rutin, which is a functional substance, compared to general buckwheat.

Rutin is a functional food additive for preventing oil and fat oxidation and maintaining the flavor of food in the food industry, and is used in confectionery, beverages, noodles, frozen foods, retort foods, and the like.

In addition, rutin is a health that performs functions such as blood pressure lowering products, anti-aging products, nitric oxide scavengers, capillary protective agents, products for preventing high blood pressure, vitamin C supplements, pulmonary bleeding preventive agents, purpura preventive agents, retinal bleeding prevention, DNA damage protective agents, etc. It is also used as a raw material for functional foods.

Rutin has a characteristic that its content increases when cultivated as sprouts by germination than in the form of buckwheat grains. Since it is poorly soluble in water, alcoholic solvents such as methanol or ethanol are generally used for extraction of rutin.

On the other hand, buckwheat contains cellulose, which is a major component of cell membranes of higher plants and is a carbohydrate that is widely distributed in a large amount in nature. It is rarely digested and excreted outside the body. However, it is known that an appropriate amount of fiber has health promotion effects such as strengthening the intestine.

As described above, buckwheat contains rutin and fiber, so it is one of the healthiest foods that it is called'herbal medicine on the table'.It is one of the food and bio industries that efficiently extracts rutin and dietary fiber from this buckwheat. There is a need for a method that can be used as a material.

1. Korean Patent Registration No. 10-0646126 (2006.11.08.) 2. Korean Patent Registration No. 10-0361632 (2002.11.06.)

After germination of buckwheat buckwheat and cultivation as a sprout, it undergoes an extraction process to be used as a material for food, etc., and the production of rutin extract using functional buckwheat sprouts for use as a material for food and bio industry as a functional substance by using the remaining residue I want to provide a way.

The method for producing a rutin extract using functional buckwheat sprouts according to the embodiment includes: 1) a washing step of washing buckwheat seeds, and 2) an immersion step of immersing the buckwheat seeds after the process of step 1) is completed for 23 to 25 hours, and , 3) a germination step of germinating the buckwheat seeds that have completed the process of step 2) for 23 to 25 hours in dark conditions, and 4) the germinated buckwheat seeds that have completed the process of step 3) at 7 to 9 under bright conditions. There is provided a method for producing a rutin extract using functional buckwheat sprouts, characterized in that buckwheat sprouts having an average length of 10 cm are obtained through a cultivation step of daily cultivation.

According to an embodiment, in the method for producing buckwheat sprouts obtained using the method for producing buckwheat sprouts of claim 1, 1) a temperature of 80 to 90 degrees Celsius by adding distilled water in an amount equal to 10 times the weight of the buckwheat sprouts. The buckwheat sprout extract, which has been heated for 12 hours, is not filtered in the step of obtaining an extract by using a filter paper and 3) obtaining an extract of the buckwheat sprout extract obtained by using a filter paper, and 3) the step of obtaining the extract of step 2). The buckwheat sprout residue drying step of obtaining a dried buckwheat sprout residue by hot air drying the uncooked buckwheat sprout residue at a temperature of 45°C to 55°C for 23 to 25 hours in a hot air machine, and 4) drying the buckwheat sprout residue of step 3). The dried buckwheat sprout residue obtained in the step is added with distilled water having a weight of 10 times the weight of the dried buckwheat sprout residue, and the step of obtaining the hot water treatment step, the step of obtaining the extract, and the step of repeating the steps of the step of drying the residue.

According to an embodiment, the buckwheat sprout is tatari buckwheat.

In the method for producing rutin extract using functional buckwheat sprouts as described above, the buckwheat sprouts are germinated and cultivated as sprouts, followed by an extraction process to be used as a material for food, etc., and the remaining residue is used as a functional substance. It has the advantage that it can be used as an industrial material.

1 is a table showing HPLC conditions for rutin analysis of a method for preparing rutin extract using functional buckwheat sprouts according to an embodiment of the present disclosure.
Figure 2 is a table showing the content of rutin analyzed under the HPLC conditions of Figure 1;
3 is an example showing the table of FIG. 2 in the form of a bar graph.
Figure 4 is a graph showing the thermal stability of the extract.

Advantages and features of the present invention, and a method of achieving them will become apparent with reference to the embodiments described later in detail together with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms, and only these embodiments make the disclosure of the present invention complete, and the general knowledge in the technical field to which the present invention pertains. It is provided to completely inform the scope of the invention to the possessor, and the invention is only defined by the scope of the claims. The same reference numerals refer to the same components throughout the specification.

In describing embodiments of the present invention, if it is determined that a detailed description of a known function or configuration may unnecessarily obscure the subject matter of the present invention, a detailed description thereof will be omitted. In addition, terms to be described later are terms defined in consideration of functions in an embodiment of the present invention, which may vary according to the intention or custom of users or operators. Therefore, the definition should be made based on the contents throughout the present specification.

The method for producing a rutin extract using functional buckwheat sprouts according to an embodiment of the present disclosure is to use tatari buckwheat, and 1) a washing step of washing buckwheat seeds, and 2) the buckwheat seeds having completed the process of step 1). A immersion step of immersing the buckwheat seeds for 23 to 25 hours, 3) a germination step of germinating the buckwheat seeds for 23 to 25 hours in dark conditions, and 4) the germination of the step 3) completed. After the cultivation step of cultivating the grown buckwheat seeds under bright conditions for 7 to 9 days, buckwheat sprouts with an average length of 10 cm are obtained.

And, in the manufacturing method of the buckwheat sprout obtained using the method for producing buckwheat sprouts of the present disclosure, 1) distilled water in an amount equal to 10 times the weight of the buckwheat sprouts is added to a temperature of 80 degrees Celsius to 90 degrees Celsius. A hydrothermal treatment step of heating for ~13 hours, 2) the extract of the buckwheat sprout extract obtained by using a filter paper, and 3) the filter paper in the step of obtaining the extract of step 2). The unfiltered buckwheat sprout residue is hot air-dried at a temperature of 45 degrees Celsius to 55 degrees Celsius for 23 to 25 hours in a hot air machine to obtain a dried buckwheat sprout residue, and 4) obtained by the step of obtaining the extract of step 3). The buckwheat sprout residue is added with distilled water 10 times the weight of the buckwheat sprout residue, and the process of the hydrothermal treatment step, the extract liquid acquisition step, and the buckwheat sprout residue drying step are repeated.

At this time, in the subsequent extraction liquid acquisition step, the dried residue is obtained by adding 10 times of distilled water again as in the aforementioned extraction process to obtain a secondary extract through the same extraction process, and the remaining residue of the secondary extract through the same process. A third extract is prepared.

At this time, it is obvious that hot water extraction is better as the temperature of the distilled water increases in the hot water extraction step, but there is a risk that the protein structure may be denatured when heated to a certain temperature or higher. As shown in Figure 4, as a result of examining the rutin content by extracting hot water at a temperature ranging from 70 degrees Celsius to 90 degrees Celsius, there is no significant difference in the rutin content, so the rutin has stability against heat even at a temperature of 90 degrees Celsius.

In addition, extraction of rutin is generally performed using alcohol solvents such as methanol or ethanol, but rutin extracted using alcohol solvents cannot be used in food, or additional equipment or processes are required to use it. , Rutin is extracted using distilled water so that it can be applied directly to food without additional equipment or processes.

Meanwhile, FIG. 1 is a table showing HPLC conditions for rutin analysis of a method for preparing rutin extract using functional buckwheat sprout according to an embodiment of the present disclosure, and FIG. 2 is a table showing rutin content analyzed under the HPLC conditions of FIG. 1; 3 is an example showing the table of FIG. 2 in the form of a bar graph.

On the other hand, the present disclosure is to evaluate the content of rutin in the primary, secondary, and tertiary extracts prepared through the process as described above, by interacting with the stationary phase (separating tube) and the sample and the mobile phase (liquid). It utilizes an analysis method through high performance liquid chromatography (HPLC), a method of separating into a single component.

Here, liquid chromatography, unlike gas chromatography, has the advantage of not only being able to analyze a non-volatile sample but also a sample having a large molecular weight because there is no limitation on the molecular weight that can be analyzed. It also has the advantage of being able to recover. In addition, liquid chromatography, like gas chromatography, is inexpensive, and for this reason, it is widely used in various fields such as food science, environmental science, and analysis of natural products as well as new drug development.

At this time, in the present disclosure, the content of rutin in the primary, secondary, and tertiary extracts was HPLC (U-3000, Thermo Fisher Scientific Inc., MA., USA) for quantitative analysis of rutin, and the standard material rutin was used. (rutin hydrate HPLC grade, ≥94%, Sigma-Aldrich, USA) was dissolved in water and used for quantitative analysis through a standard curve. At this time, the HPLC conditions for the routine analysis of the present disclosure are as shown in the table shown in FIG. 1, and the mobile phase was analyzed in an isocratic state in a ratio of methanol: acetonitrile: acetic acid 2.5% 2:2:6. .

According to the present disclosure as described above, as shown in Figs. 2 and 3, the rutin content of the residue is extracted for 12 hours using 10-fold hot water extraction on the shoots in the case of the primary extract, and the rutin content is 217.2758 ppm. Was measured. In addition, in the case of the secondary extract, the rutin content was measured to be 268.7013 ppm, and the reason that the secondary extract showed a higher content than the primary extract was that the primary extract was made from undried shoots, so the water content was high. This is because the amount of shoots that are actually extracted is small (the moisture content of the shoots reaches about 95%). And, the third extract was measured to be 62.014ppm.

On the other hand, in the present disclosure, the dietary fiber analysis of the primary, secondary, and tertiary extracts was performed using the NDF (Natural Detergent Fiber) method. This method is a method of applying dissolution and aerosolization. Samples suitable for this method are suitable for green leafy vegetables, roots, and wild vegetables, and starch-rich samples must be separately decomposed and removed. If it contains a lot of pectin, it is determined separately. shall.

To this end, in the present disclosure, the residue of the primary extract is pulverized and 1 g is placed in a 500 ml Erlenmeyer flask, and 100 ml of NDF reagent and 1 ml of isoamyl alcohol are added and boiled for 60 minutes. After that, the remaining material was filtered through a suction filtration device, dried in a 100°C drying oven, and incinerated for 1 hour, and the weight was measured.

According to the present disclosure according to the above, the content of dietary fiber was measured to be 2.10%.

In the method for producing rutin extract using functional buckwheat sprouts as described above, the buckwheat sprouts are germinated and cultivated as sprouts, followed by an extraction process to be used as a material for food, etc., and the remaining residue is used as a functional substance. It has the advantage that it can be used as an industrial material.

The disclosed contents are only examples, and various changes may be made by those of ordinary skill in the art without departing from the gist of the claims claimed in the claims, so the scope of protection of the disclosed contents is limited to the above-described specific It is not limited to the examples.

Claims (3)

In the manufacturing method of rutin extract using functional buckwheat sprout,
1) a hydrothermal treatment step of heating for 11 to 13 hours at a temperature of 80 to 90 degrees Celsius by adding distilled water in an amount equal to 10 times the weight of the buckwheat sprouts;
2) an extract obtaining step of obtaining an extract from the buckwheat sprout extract after the process of step 1) is completed using a filter paper;
3) Drying the buckwheat sprout residue, which was not filtered in the step of obtaining the extract of step 2), with hot air drying for 23 to 25 hours at a temperature ranging from 45 degrees Celsius to 55 degrees Celsius in a hot air blower to obtain the dried buckwheat sprout residue. step;
4) A subsequent extract that repeats the processes of the hot water treatment step, the extract obtaining step, and the residue drying step by adding 10 times the weight of distilled water to the dried buckwheat sprout residue obtained in the drying step of the buckwheat sprout residue in step 3). Obtaining step; Method for producing a rutin extract using a functional buckwheat sprout, characterized in that consisting of.
The method of claim 1, wherein the buckwheat sprout,
1) washing step of washing buckwheat seeds;
2) an immersion step of immersing the buckwheat seeds on which the process of step 1) is completed for 23 to 25 hours;
3) a germination step of germinating the buckwheat seeds on which the process of step 2) is completed for 23 to 25 hours in dark conditions;
4) A cultivation step of cultivating the germinated buckwheat seeds after the process of step 3) is completed for 7 to 9 days under bright conditions; buckwheat sprouts having an average length of 10 cm are obtained. Manufacturing method.
The method according to claim 1 or 2,
The buckwheat sprout is a method for producing a rutin extract using functional buckwheat sprouts, characterized in that the buckwheat sprouts are tatari buckwheat sprouts.

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