KR20210048678A - Cosmetic composition comprising extract of Tilia mandshurica - Google Patents

Abstract

The present invention relates to an anti-pollution cosmetic composition containing a Tilia mandshurica extract. The Tilia mandshurica extract has an effect of alleviating or protecting skin cell damage against aldehyde complexes, thereby being usefully used as a cosmetic composition for maintaining skin health from environmental harmful factors such as fine dust and yellow dust.

Description

Cosmetic composition comprising extract of Tilia mandshurica}

The present invention relates to an anti-pollution cosmetic composition containing a chamomile extract, and more particularly, to an anti-pollution cosmetic composition containing a chamomile extract characterized by improving or protecting skin damage to an aldehyde complex. .

As a body part that is directly exposed to the external environment, the skin not only acts as a protective film to protect important organs of our body, but also controls water evaporation and protects the body from external infections. However, no matter how much the skin prevents the penetration of viruses from the outside, stress received from the outside, such as excessive ultraviolet rays and contaminated environments, induces skin irritation and eventually leads to skin aging.

Fine dust refers to small dust with a diameter of less than 10 μm, which is not visible as an airborne substance with various components, and contains toxic substances such as aldehydes, sulfates, and nitrates. In addition, fine dust is produced by photochemical reactions in the atmosphere of yellow dust, automobile fumes, and heavy metals discharged from factory chimneys, and when exposed to a high concentration of fine dust, coughing, eye irritation, skin troubles, and lung inflammation occur.

The fine dust contains volatile organic compounds (VOCs), and these volatile organic compounds collectively refer to liquid or gaseous organic compounds that have a low boiling point and are easily evaporated into the atmosphere. Volatile organic compounds are very diverse, from solvents commonly used in industries to organic gases emitted from plastic drying processes, and most of the hydrocarbons commonly used in the vicinity, such as low boiling point liquid fuels, paraffins, olefins, and aromatic compounds ( http://www.me.go.kr/mamo/web/index.do?menuId=10007, Ministry of Environment Metropolitan Air Quality Administration).

Formaldehyde (FA), a representative pollutant among volatile organic compounds, has an irritating odor and is a colorless, water-soluble gas and is used in the production of urea and phenolic synthetic resins. Formaldehyde causes irritation to the mucous membranes and skin of the upper respiratory tract, eyes, etc., and long-term exposure has been reported to be associated with the development of respiratory obstructive pulmonary disease and lung cancer (Callas, 1996, J Occup Environ Med ).

On the other hand, most of the fine dust also contains heavy metals, and heavy metals refer to heavy metal elements with a specific gravity of 4 or more, such as lead, mercury, cadmium, zinc, and nickel, and these heavy metals are not well excreted when accumulated in the body even in trace amounts and It is recognized as very dangerous because it accumulates and exhibits side effects over a long period of time. In addition, it has been reported that chemical reactions in the air can cause skin irritation, atopy, and skin troubles by additionally generating harmful substances such as nitrogen oxides (NO).

Currently, the method of removing heavy metals in the body is known as a method of discharging heavy metals out of the body by injecting complex compound-forming substances such as EDTA (ethylene diamine tetraacetate) and BAL (British Anti-Lewisite) to bind with the heavy metals accumulated in the body. There is no known effective and easy way to protect skin from heavy metals so far.

Tilia mandshurica (manchurian linden) is a tall tall tree of the hempaceae family and grows up to 20m in height. In Korea, it is distributed in mountainous areas all over the country except Jeju Island. The fruit is a nut, round, with a little angular line at the bottom, dense hairs, and ripens in September to October. It is also planted as a garden tree, wood is used as furniture, bark is a fiber resource, and flowers provide honey to bees. In oriental medicine, flowers are used as a medicinal material, which has an antipyretic effect by making sweat and is also used as a sedative.

As a result of research efforts on a new natural substance that can protect the skin from environmentally harmful factors, which are recently in the spotlight, the present inventors have found that the extract of the grass bark has very excellent efficacy in inhibiting cell damage from aldehyde complexes, which are environmentally harmful substances. By confirming, the present invention was completed.

Republic of Korea Patent Registration No. 10-1464406 (Name of the invention: Composition containing a mulberry leaf extract or a fraction thereof as an active ingredient and its use, Applicant: Cheju National University Industry-Academic Cooperation Foundation, registration date: November 17, 2014) Republic of Korea Patent Registration No. 10-1862093 (Name of the invention: A composition containing extracts of mulberry, cypress, chinensis, and dysentery, and has complex antioxidant and whitening functions, Applicant: Soonchunhyang University Industry-Academic Cooperation Foundation, Registration Date: May 2018 23rd month) Korean Patent Registration No. 10-0811729 (Name of invention: Lifting cosmetic composition that promotes fat decomposition, Applicant: MEL Co., Ltd., registration date: March 3, 2008) Republic of Korea Patent Publication No. 10-2003-0090320 (Name of invention: whitening cosmetic composition, Applicant: Korea Kolmar Co., Ltd. and one other, Publication date: January 21, 2019) Republic of Korea Patent Publication No. 10-2019-0006888 (Name of the invention: Hwanggeun extract, a composition for skin anti-pollution comprising a compound isolated from a fraction of Hwanggeun extract and a fraction of Hwanggeun extract, Applicant: Nadri Cosmetics Co., Ltd., Publication date: November 28, 2003)

Kim Chang-min et al., Study on the composition of the Korean genus Bark-The composition of the bark of the bark of the Japanese bark, The Korean Society of Pharmacy, Vol. 19, No. 3, p174-176, 1988. Callas, 1996, J Occup Environ Med.

It is an object of the present invention to provide a cosmetic composition having an anti-pollution function containing a chamois tree extract. In more detail, it is an object of the present invention to provide an anti-pollution cosmetic composition containing a chamois extract having a function of improving or protecting skin damage to an aldehyde complex.

The present invention relates to a cosmetic composition having an anti-pollution function containing an extract of Tilia mandshurica (manchurian linden).

The chamois tree extract is characterized in that it has an anti-pollution function that suppresses the death of skin cells due to environmentally harmful factors.

The skin cells may include keratinocytes.

The environmental harmful factors may include aldehydes or heavy metals.

The aldehydes may be at least one selected from the group consisting of formaldehyde, acetalaldehyde, propionaldehyde, and benzylaldehyde.

The aldehydes or heavy metals may be contained in fine dust.

The extract may be extracted from the grass bark using water, a lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, or a mixture thereof as an extraction solvent.

Hereinafter, the present invention will be described in detail.

The present invention relates to a cosmetic composition having an anti-pollution function containing a chamois tree extract, and may have an effect of improving or protecting skin damage due to the anti-pollution function. As a raw material sample for the extract, leaves may be used, or a mixture of leaves and branches may be used, and the leaves and branches may be mixed in a weight ratio of 1:0.5-2.

The extract may be extracted using water, a lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, or a mixture thereof as an extraction solvent. The lower alcohol having 1 to 4 carbon atoms may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol.

The extraction solvent used in the preparation of the extract may be 1 to 60 times the volume (1 to 60 liters per 1 kg) based on the weight of the mixture, and preferably 5 to 60 times the volume. Extraction conditions for the extract may be 1 minute to 48 hours at 20 ~ 100 ℃. The process can be repeated up to 1 to 4 times. At this time, ultrasonic treatment may be performed during the extraction process.

In addition, as a conventional method in the art, after dissolving the extract in water, fractions were additionally fractionated using one or more solvents selected from the group consisting of n-hexane, methylene chloride, acetone, chloroform, ethyl acetate, and n-butanol. It can be manufactured with. In addition, according to the commercial method, extraction of organic solvents (alcohol, ether, acetone, hexane, methylene chloride, ethyl acetate, etc.), distribution of organic solvents such as hexane and butanol and water, separation of plant components, etc. It can be used after fractionation or purification using a method alone or a suitable combination of known methods used for extraction.

The manufacturing temperature of the extract may be 20 to 100 ℃, but is not limited thereto. The extraction time is not particularly limited, but it is preferable to extract within 10 minutes to 2 days, and as an extraction device, a conventional extraction device, an ultrasonic grinding extractor, or a fractionator may be used.

When preparing the extract of the present invention, when filtering the extract, the filtration is a process of removing suspended solid particles from the extract. The particles are filtered using cotton, nylon, paper, etc., or ultrafiltration, freeze filtration, centrifugation, etc. It can be used, but is not limited thereto.

The extraction method in the preparation of the extract of the present invention is not particularly limited, and according to the type and extraction method of the extract, for example, cold needle extraction, ultrasonic extraction, reflux cooling extraction, room temperature extraction, hot water extraction, and the like may be used.

The prepared extract includes, but is not limited to, hot air drying, vacuum drying, freeze drying, vacuum drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying, and the like. In some cases, a process of pulverizing the final dried extract may be added.

In addition, the extract can be used after purification using column chromatography. The chromatography includes silica gel column chromatography, LH-20 column chromatography, ion exchange resin chromatography, and medium pressure liquid chromatography. chromatography), thin layer chromatography (TLC), silica gel vacuum liquid chromatography, and high performance liquid chromatography.

The extract of the present invention is characterized by having an effect of inhibiting increased cell death due to environmental harmful factors. The environmental harmful factors may be aldehydes, and the aldehydes may be at least one selected from the group consisting of formaldehyde, acetalaldehyde, propionaldehyde, and benzylaldehyde. In addition, the environmentally harmful factors may be fine dust, vehicle exhaust gas, yellow dust, benzopyrene or heavy metals, and aldehydes may be included or mixed with these environmentally harmful factors. In addition, the heavy metals are metals having a specific gravity of 4 or more, and may have a specific gravity of up to 23, such as mercury (13.5), cadmium (8.7), lead (11.3), and copper (8.9). The environmentally harmful factor may include an aldehyde.

The present invention can provide a cosmetic comprising the cosmetic composition. The cosmetic composition may contain 0.001 to 20% by weight of the cosmetic composition containing the extract, based on the total weight of the cosmetic composition 100% by weight.

The formulation of the cosmetic is not particularly limited, but preferably skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence , Nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

The cosmetic composition of the present invention may further include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

The water-soluble vitamin may be any one that can be blended in cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, etc. And their salts (thiamine hydrochloride, sodium ascorbate, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-magnesium salt, etc.) can also be used in the water-soluble vitamins that can be used in the present invention. Included. Water-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a culture of microorganisms, an enzyme method, or a chemical synthesis method.

As an oil-soluble vitamin, any one can be used as long as it can be blended into a cosmetic, but preferably, vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), etc. are mentioned. , Their derivatives (ascorbine palmitate, ascorbine stearate, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinic acid, vitamin E, DL-pantotenyl alcohol, D-pantotenyl alcohol, pantotenyl ethyl Ether, etc.) are also included in the oil-soluble vitamin used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as a microbial transformation method, a purification method from a culture of microorganisms, an enzyme or chemical synthesis method.

The polymer peptide may be any one as long as it can be blended into a cosmetic, but preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like are exemplified. The polymeric peptide can be purified and obtained by a conventional method such as a purification method from a culture medium of a microorganism, an enzyme method, or a chemical synthesis method, or it can be used after being purified from natural products such as dermis of pigs and cattle, silk fibers of silkworms, etc.

As the polymeric polysaccharide, any one can be used as long as it can be blended in a cosmetic, but preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfuric acid, or a salt thereof (sodium salt, etc.) are exemplified. For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

The sphingo lipid may be any one as long as it can be blended into a cosmetic, but preferably, ceramide, phytosphingosine, sphingoglycolipid, or the like can be mentioned. Sphingo lipids are usually purified from mammals, fish, shellfish, yeast, plants, etc. by a conventional method or can be obtained by chemical synthesis.

The seaweed extract may be any one as long as it can be blended in cosmetics, but preferably brown algae extract, red algae extract, green algae extract, etc. are mentioned.Also, carrageenan, arginic acid, sodium arginate purified from these seaweed extracts, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying from seaweed by a conventional method.

In the cosmetic composition of the present invention, other ingredients to be blended in conventional cosmetics may also be blended.

Other ingredients that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, Blood circulation accelerators, cold sensation agents, restrictors, and purified water.

Examples of oil and fat components include ester oils and fats, hydrocarbon oils, silicone oils, fluorine oils, animal oils and vegetable oils.

As ester-based fats and oils, tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, adipine Diisopropyl acid, isoalkyl neopentanoate, glyceryl tri(caprylic, capric acid) glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, pentaelisitol tetra2-ethylhexanoate , Cetyl caprylate, decyl laurate, hexyl laurate, decyl myristic acid, myristic myristic acid, cetyl myristate, stearyl stearate, decyl oleate, ricinooleate cetyl, isostea laurate Reel, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate cetoste Aryl, 2-ethylhexanoate stearyl, isostearate hexyl, dioctanoate ethylene glycol, dioleate ethylene glycol, dicaprylic acid propylene glycol, di(caprylic acid) propylene glycol, dicaprylic acid propylene glycol, dica Neopentyl glycol prinate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate , Octyl isononate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerololeic acid ester, polyglycerin isostearic acid ester, Triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, di malic acid Isostearyl, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, Cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, pitsteryl isostearate, pitsteryl oleate, 12-stealoyl And esters such as isocetyl hydroxystearate, stearyl 12-stealoylhydroxystearate, and isostearyl 12-stealoylhydroxystearate.

Examples of hydrocarbon-based fats and oils include hydrocarbon-based fats such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, and petrolatum.

Silicone-based fats and oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane/methylcetyloxysiloxane copolymer, dimethylsiloxane/methylsteaoxysiloxane copolymer, alkyl And modified silicone oil and amino-modified silicone oil.

As fluorine-based fats and oils, perfluoropolyether, etc. are mentioned.

Animal or plant oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, bird flower oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed Oil, Palm Oil, Cucuine Nut Oil, Wheat Germ Oil, Rice Germ Oil, Shea Butter, Walnut Colostrum Oil, Marker Demi Anut Oil, Meadow Home Oil, Egg Yolk Oil, Tallow Oil, Horse Oil, Mink Oil, Orange Lapi Oil, Jojoba Oil , Animal or plant fats such as canderry wax, carnauba wax, liquid lanolin, and hydrogenated castor oil.

Examples of the moisturizer include water-soluble low-molecular moisturizers, fat-soluble molecular moisturizers, water-soluble polymers, and fat-soluble polymers.

As water-soluble low molecular weight moisturizers, serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), polypropylene Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactate, and the like.

As a fat-soluble low molecular weight moisturizer, cholesterol, cholesterol ester, etc. are mentioned.

Examples of water-soluble polymers include carboxyvinyl polymer, polyaspartic acid salt, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, and the like. I can.

Examples of the oil-soluble polymer include polyvinylpyrrolidone/eicosene copolymer, polyvinylpyrrolidone/hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollient agent include long-chain acyl glutamate cholesteryl ester, hydroxystearate cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, and the like.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hydrogenated castor oil, POE castor oil, POE·POP (polyoxyethylene·polyoxypropylene) copolymer, POE·POP alkyl ether, polyether-modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactants, fatty acid soap, alpha-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkyl amide sulfate, alkyl phosphate, POE alkyl phosphate, alkyl amide Phosphate, alkyloylalkyltaurine salt, N-acylamino acid salt, POE alkylether carboxylate, alkylsulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkylphosphoric acid ester, etc. have.

Cationic surfactants include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyldimethylbenzyl ammonium chloride, behenyl trimethyl ammonium bromide, and chloride. Benzalkonium, diethylaminoethyl amide stearate, dimethylaminopropyl amide stearate, lanolin derivative quaternary ammonium salt, and the like.

Examples of amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, amideamine type, etc. And amphoteric surfactants.

Examples of organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, and aluminum oxide. , Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine, chromium oxide, chromium hydroxide, calamine, and complexes thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene-styrene copolymer, Organic pigments, such as silk powder, cellulose, CI pigment yellow, and CI pigment orange, and complex pigments of these inorganic pigments and organic pigments, etc. are mentioned.

Examples of the organic powder include metal soaps such as calcium stearate; Alkyl phosphate metal salts, such as sodium zinc cetylate, zinc laurylate, and calcium laurylate; Acylamino acid polyvalent metal salts, such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroyl glycine calcium; Amide sulfonic acid polyvalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N, such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoyl lizine, N-alpha-paritoylolnitine, N-alpha-lauroylarginine, N-alpha-hardened tallow fatty acid acylarginine, etc. -Acyl basic amino acid; N-acyl polypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene/styrene copolymer, ethylene tetrafluoride, and the like.

UV absorbers include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicate, butyl phenyl salicylate, homomentyl salicylate, benzyl cinnamate. , Paramethoxycinnamic acid-2-ethoxyethyl, paramethoxycinnamic acid octyl, diparamethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxycinnamic acid isopropyl, diisopropyl·diisopropyl cinnamic acid ester mixture, right Cannic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1 ,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

As a disinfectant, hinokitiol, triclosan, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinphylithione, benzalkonium chloride, photosensitization Sodium No. 301, mononitroguarous sodium, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and elisorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, pmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Examples of alcohol include higher alcohols such as cetyl alcohol.

In addition, the blending ingredients that may be added are not limited thereto, and any of the above ingredients can be blended within a range that does not impair the object and effect of the present invention, but is preferably 0.01 to 5% by weight, more preferably, based on the total weight. May be blended in 0.01-3% by weight.

The cosmetic composition of the present invention may take the shape of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetics in addition to the above extracts as active ingredients, for example, conventional auxiliary agents and carriers such as stabilizers, solubilizers, vitamins, pigments and fragrances. Includes.

When the cosmetic formulation of the present invention is a paste, cream or gel, animal fibers, plant fibers, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide are used as carrier components. Can be.

When the cosmetic formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, Propellants such as propane/butane or dimethyl ether.

When the cosmetic formulation of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the cosmetic formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol as carrier components, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, crystallites Sex cellulose, aluminum metahydroxide, bentonite, agar or tracanth, and the like can be used.

When the cosmetic formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty diethanolamides, vegetable oils, linoline derivatives or ethoxylated glycerol fatty acid esters may be used.

In yet another aspect, the present invention provides a pharmaceutical composition for improving or protecting skin damage having an anti-pollution function, containing the extract of the chamomile tree. The content of the extract contained in the pharmaceutical composition is not particularly limited. The pharmaceutical compositions may be formulated and used in the form of oral dosage forms, such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and sterile injectable solutions, respectively, according to a conventional method. Carriers, excipients, and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oils. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations include at least one excipient, such as starch, calcium carbonate, sucrose or lactose, in the extract of the present invention, It is prepared by mixing gelatin or the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, liquid solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as humectants, sweeteners, fragrances, and preservatives may be included. . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized formulations, and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

The dosage of the pharmaceutical composition of the present invention will vary depending on the age, sex, and weight of the subject to be treated, the specific disease or pathological state to be treated, the severity of the disease or pathological state, the route of administration, and the judgment of the prescriber. Dosage determination based on these factors is within the level of one of skill in the art, and dosages generally range from 0.01 mg/kg/day to approximately 2000 mg/kg/day. A more preferred dosage is from 1 mg/kg/day to 500 mg/kg/day. Administration may be administered once a day, or may be divided several times. The above dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention can be administered to mammals such as mice, livestock, and humans by various routes. All modes of administration can be expected and can be administered, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or cerebrovascular injection. Since the extract of the present invention has little toxicity and side effects, it is a drug that can be safely used even when taken for a long time for prophylactic purposes.

In addition, the present invention provides a health functional food for improving or protecting skin damage with an anti-pollution function, including a chalcedony tree extract and a food supplementary additive. The Chalpwood extract may be added in an amount of 0.001 to 100% by weight to the health functional food of the present invention. The health functional food of the present invention includes forms such as tablets, capsules, pills or liquids, and foods to which the extract of the present invention can be added include, for example, various drinks, meats, sausages, bread, candy, There are snacks, noodles, ice cream, dairy products, soups, ion drinks, beverages, alcoholic beverages, gum, tea and vitamin complexes.

The present invention relates to an anti-pollution cosmetic composition containing a chamois tree extract. The chamois tree extract can be usefully used as a cosmetic composition for maintaining skin health from environmentally harmful factors such as fine dust and yellow dust because it has an effect of improving or protecting skin cells against an aldehyde complex.

Figure 1 is a graph showing the cell viability of the extract of keratinocytes against keratinocytes.
2 is a graph showing cell viability by concentration of an aldehyde complex for keratinocytes.
Figure 3 is a graph showing the cell survival rate after pretreatment of keratinocytes extract from the keratinocytes and treatment with an aldehyde complex.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to sufficiently convey the spirit of the present invention to those skilled in the art so that the contents introduced herein are thorough and complete.

<Example 1. Preparation of barley tree extract>

The leaves of Tilia mandshurica used as a sample were washed with distilled water. The sample was finely pulverized using a harvested bean and extracted using a 70% (v/v) ethanol aqueous solution. In the extraction process, the sample was sufficiently immersed in an aqueous solution of 70% (v/v) ethanol 10 times the volume of 100 g of the sample, followed by extraction while stirring at room temperature (25° C.) for 24 hours. After collecting the extract, An aqueous ethanol solution was added to the remaining sample, and the same process was repeated to perform extraction a total of 3 times.

The extract obtained by this method was first filtered using sterile gauze, and the filtrate was filtered again using a filter paper (Whatman No. 2: Watman Ltd., Maidstone, Kent, UK) to filter out pulverized samples and the like. Next, the filtrate was concentrated under reduced pressure at room temperature (25° C.) using a rotary vacuum concentrator, and the remaining solvent was removed by lyophilization to obtain an extract. The obtained lyophilized extract was stored at -20°C, and the extract obtained by this method was dissolved in DMSO (dimethyl sulfoxide) whenever necessary for the experiment and used.

<Example 2. Confirmation of cytotoxicity of the extracts of the barberry tree itself>

HaCaT (Human ketatinocyte) was used to confirm the cytotoxicity of the extracts of the chamomile tree itself. To this end, keratinocytes were cultured in a 96-well plate using DMEM (Dulbeco's Modified Eagle's Media) containing 10% (v/v) fetal bovine serum (FBS) for 18 hours, and then keratinocytes without FBS. It was replaced with 154CF, a sterile liquid medium for culture. Next, the extract prepared in Example 1 was treated with 100 µg/ml in each well and cultured for 24 hours. Thereafter, 10 µl of CCK-8 reagent (Dojindo) was added per well, incubated for 3 hours, and absorbance was measured at 450 nm. The value of% was confirmed and it was confirmed that there was no cytotoxicity.

<Example 3. Confirmation of Cytotoxicity of Aldehyde Complex>

The same experiment as in Example 2 was carried out, but instead of treating the extract of the Serrata after replacing the 154CF medium, an aldehyde complex containing formaldehyde, acetalaldehyde, propionaldehyde, and benzylaldehyde in the same molar ratio was prepared by concentration (0.05, 0.1, 0.2, 0.5, 1, 2 mM), and cultured for 24 hours, and cytotoxicity was confirmed with CCK-8 reagent.

As a result, as shown in FIG. 2, when the aldehyde complex was treated with 1 to 2 mM, the cell viability was lowered to about 23 to 28%, and it was confirmed that this concentration is suitable for use as a cell damage model.

<Example 4. Cytotoxicity confirmation of aldehyde complex>

The same experiment as in Example 2 was carried out, but after the replacement of the 154CF medium, after the 154CF medium was replaced, each well was first treated with 100 µg/ml for 30 minutes, and then the aldehyde complex was mixed to 1 mM in the culture medium treated with the serrata. Incubated again for 24 hours, and cytotoxicity was confirmed with CCK-8 reagent.

As a result, as shown in FIG. 3, in the group treated with the aldehyde complex alone, the cell survival rate was lowered to 22.4%, but it was confirmed that the survival rate of keratinocytes recovered to 71.3% in the group treated with the extract of keratinocytes.

<Example 5. Tilia Comparison of anti-pollution efficacy of genus plants>

After preparing an extract by the method of Example 1 using Tilia amurensis and Tilia taquetii , cytotoxicity was confirmed at a concentration of 100 μg/ml as in Example 4, and the results shown in Table 1 below. Got it.

sample Cell viability (%) Untreated group 100.0 The extract of the chamomile tree of Example 1 71.3 Blood tree extract 29.1 Mulberry leaf extract 34.1

<Example 6. Measurement of heavy metal adsorption property>

Adsorption of heavy metals in the extract of the grasshopper [Journal of the Korea Academia-Industrial cooperation Society Vol. 13, No. 2 pp. 934-940, 2012] was measured as follows. Each 100 ml of 20 ppm lead and cadmium ion solution was taken into an Erlenmeyer flask, 0.5 to 1 g of sample powder was injected thereto, and then adsorbed by shaking at a speed of about 200 rpm in a shaker. After a certain period of time after adsorption, shaking was stopped, and vacuum suction was filtered using a 0.45 μm membrane filter paper. At this time, heavy metal ions adhering to the inner wall of the Erlenmeyer flask and the surface of the adsorbent were washed several times with distilled water and filtered. Each filtrate containing ions was quantified to 200 ml, and the amount of heavy metals contained in the filtrate was quantified by ICP-OES (Inductively Coupled Plasma-OES, TJA polyscan 61E). Converted. In addition, after adsorption, the heavy metal adsorbed on the filter paper was placed in a 250 mL Erlenmeyer flask with 50 mL of 0.5N nitric acid solution while on the filter paper, and shaken at 200 rpm for 30 minutes to recover the adsorbed heavy metal. The shaken solution was filtered through a filter paper, and the amount of heavy metal contained in the filtrate was measured by AA (Atomic Absorption Spectrophotometer, contrAA-300, analytik-jena, Germany). At this time, the concentrations of Pb(II) and Cd(II) ions were measured at wavelengths of 228.8 nm and 324.0 nm, respectively, and the recovery rate of the Pb(II) and Cd(II) ions adsorbed to the extract of the Pb(II) ions obtained as a result. Is shown in Table 2.

Time(min) Recovery rate (%) Pb(II) Cd(II) One 42 13 2 51 19 5 59 28 10 68 39 30 80 50 60 86 57

Referring to the results of Table 2, it can be seen that the recovery rate of lead and cadmium ions remarkably increases as the adsorption reaction time of the extracts of the barberry increases. Through this result, it can be confirmed that when the extract of the chamomile tree is supplied to the skin as a cosmetic composition, it has a function of protecting skin cells by adsorbing heavy metals in the atmosphere.

<Cosmetic formulation example 1. Preparation of flexible lotion>

As shown in the composition of Table 3 below, a flexible lotion (skin, 100g) containing the extract obtained in Example 1 was prepared according to a conventional method.

Raw material Content (g) Example 1 extract 0.3 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Polyoxyethylene (60) hardened castor oil 1.0 ethanol 10.0 Triethanolamine 0.1 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 2. Preparation of nutrient lotion>

As shown in the composition of Table 4 below, a nutrient lotion (lotion, 100 g) containing the extract obtained in Example 1 was prepared according to a conventional method.

Raw material Content (g) Example 1 extract 0.1 Sitosterol 1.7 Polyglyceryl 2-oleate 1.5 Ceteares 1.2 cholesterol 1.5 Dicetylphosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 10.0 Carboxyvinyl polymer 0.2 Xanthan gum 0.3 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 3. Preparation of nutrient cream>

As shown in the composition of Table 5 below, a nutritional cream (100g) containing the extract obtained in Example 1 was prepared according to a conventional method.

Raw material Content (g) Example 1 extract 0.5 Sitosterol 4.0 Polyglyceryl 2-oleate 3.0 Ceramide 0.7 Ceteares-4 2.0 cholesterol 3.0 Dicetylphosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic Formulation Example 4. Preparation of Essence>

As shown in the composition of Table 6 below, an essence (100 g) containing the extract obtained in Example 1 was prepared according to a conventional method.

Raw material Content (g) Example 1 extract 0.5 Sitosterol 1.7 Polyglyceryl 2-oleate 1.5 Ceteares-4 2.0 cholesterol 3.0 Dicetylphosphate 0.4 Concentrated glycerin 5.0 Sunflower Oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

<Cosmetic formulation example 5. Preparation of foundation>

As shown in the composition of Table 7 below, a foundation (100 g) containing the extract obtained in Example 1 was prepared according to a conventional method.

Raw material Content (g) Example 1 extract 0.2 Beeswax 2.0 Cyclomethicone 2.0 Floating paraffin 5.0 Squalane 5.0 Stearic acid 2.0 Lipophilic glycerin monostearate 3.0 Caprylic/Capric Triglyceride 4.0 glycerin 4.0 Propylene glycol 3.0 Butylene glycol 3.0 Triethanolamine 1.0 Aluminum magnesium silicate 0.5 Pigment 12.0 antiseptic a very small amount Spices a very small amount Purified water Balance

Claims (6)

Chalpwood ( Tilia mandshurica , manchurian linden) anti-pollution cosmetic composition comprising the extract. The method of claim 1,
The anti-pollution cosmetic composition, characterized in that the extract of the chalcedony tree has an effect of inhibiting the death of skin cells due to environmentally harmful factors. The method of claim 1,
Anti-pollution cosmetic composition, characterized in that the skin cells are keratinocytes. The method of claim 1,
The environmentally harmful factor is an anti-pollution cosmetic composition, characterized in that it contains aldehydes or heavy metals. The method of claim 4,
Anti-pollution cosmetic composition, characterized in that the aldehydes or heavy metals are contained in fine dust. The method of claim 1,
The extract is an anti-pollution cosmetic composition, characterized in that the extract is extracted with water, lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, or a mixture thereof as an extraction solvent.

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