KR20210011672A - The extracting method of Rubus coreanus and Cosmetic composition containing active fractions of Rubus coreanus - Google Patents

Abstract

The present invention relates to a method for processing a mushroom fortified with vitamin D2 by irradiating LED UV-B to King oyster mushroom or Pleurotus ostreatus. More specifically, the present invention relates to a method for processing a mushroom fortified with vitamin D2 comprising the following steps: 1) washing King oyster mushroom or Pleurotus ostreatus in running water for 10 minutes; 2) drying the same to make 8 to 1% of a moisture content after completing washing; 3) slicing or powdering the dried King oyster mushroom or Pleurotus ostreatus; 4) irradiating LED UV-B to the powdered or sliced mushroom for 10 to 15 minutes; and 5) inducing biosynthesis by leaving mushrooms irradiated with LED UV-B at a temperature of 5 to 18°C for 1.3 to 3 hours.

Description

The vit-D enhancement method of mushrooms using LED UV-B {The extracting method of Rubus coreanus and Cosmetic composition containing active fractions of Rubus coreanus}

The present invention is one of sterols contained in fungal microorganisms such as oyster mushroom and oyster mushroom, and converted to ergocalciferol (vitamin D) by irradiation with LED UV-B on ergosterol, a precursor of vitamin D2. This is an invention related to a method of processing mushrooms enriched with vitamin D2 using LED UV-B.

Oyster mushrooms are the most produced mushrooms, accounting for 35.1% of the production of mushrooms grown in Korea, and have been reported to have excellent nutritional properties, as well as anti-cancer effects, lowering cholesterol, and antioxidant effects. In addition, as a species that grows wildly throughout Central Asia, the Middle East, and the Mediterranean region, it was introduced to Korea in the early 90s, and the name'single mushroom' seems to have been attached to the meaning of'a mushroom similar to a newly introduced pine tree'. The exact name is'big oyster mushroom'. These new pine mushrooms have immunity and anticancer effects, and also strengthen digestion. In addition, as it is rich in vitamin B6, it is effective in improving skin problems such as acne and dry dermatitis, and the pine oyster mushroom is rich in fiber and moisture, low in calories, and is in the spotlight as a diet food because of its satiety.

Vitamin D has two important forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Vitamin D2 is synthesized from yeast and ergosterol, a plant sterol, and vitamin D3 is a precursor of cholesterol in the skin, 7-D. It can be synthesized from hydrocholesterol (7-dehydrocholesterol). In addition, since vitamin D is important for maintaining various physiological functions, a lack of vitamin D increases the risk of disease development. In chronic neurodegenerative diseases, many risk factors for vitamin D deficiency appear, such as aging, obesity, lack of sunlight exposure, living in high latitude areas, and dark skin. Vitamin D deficiency not only increases the incidence of osteoporosis, falls, and hip fractures, but also increases various cancers and autoimmune diseases.If vitamin D is deficient, insulin action becomes dull, causing abdominal obesity, and a weight control point that keeps weight constant. Increases fat mass. Therefore, if vitamin D is insufficient, the incidence of diseases such as obesity, diabetes, heart disease, osteoporosis, degenerative arthritis, and colon cancer increases. In addition, vitamin D was found to increase the risk of rickets, osteoporosis, and multiple sclerosis if not sufficiently supplied as a nutrient essential for bone growth.

However, recently, when exposed to UV rays, vitamin D biosynthesis in the skin is hampered by using various sunscreens or sunscreen products to block UV rays due to concerns about skin aging or skin cancer. . In particular, since menopausal women and the elderly may be extremely limited in vitamin D biosynthesis if they receive less UV irradiation, it is inevitable to supply adequate vitamin D through food, so technologies related to food development containing a large amount of vitamin D are emerging. .

For example, in Korean Patent No. 1663143, a method for obtaining vitamin D2 from shiitake is characterized by comprising adding alcohol to shiitake powder, followed by reflux extraction to obtain shiitake extract, and irradiating UV to the shiitake extract. Is disclosed, and domestic registration patent No. 1168747 divides oyster mushroom into inoculation stage, germination induction stage, and growth stage, and then ergo by irradiating blue visible light or green visible light through LED lighting according to each stage. An invention related to a method for cultivating oyster mushrooms with enhanced sterol content is disclosed, and in Korean Patent No. 0637833, after slicing the mushrooms, UV-resistant at a temperature of 25 to 35°C and a relative humidity of 60 to 80% is disclosed. A method for producing mushrooms with increased vitamin D2 content by irradiating B is disclosed, and Korean Patent No. 1588105 discloses germinated brown rice with an LED (light emitting diode) to produce germinated brown rice with increased antioxidant activity compared to the control. A method is disclosed, and a method for producing oyster mushrooms having an increased content of vitamin D2 without color change by irradiating UV-B with an intensity of 0.9 to 1.2 W/m2 to oyster mushrooms has been developed in Korean Patent No. 1349615.

However, as a result of analyzing the above-described prior inventions, it was found that the synthesis of vitamin D2 was very insignificant when conventional ultraviolet rays were irradiated on mushrooms. Accordingly, the inventors of the present invention have found that when the mushrooms are irradiated with LED UV-B (LED mid-wavelength ultraviolet rays), the synthesis of vitamin D2 is more difficult than when irradiating conventional ultraviolet rays or LED UV-A or LED UV-C. It was confirmed to increase and the present invention was completed.

Domestic Patent No. 1663143 Domestic registered patent No. 1168747 Domestic registered patent No. 0637833 Domestic registered patent 1349615 Domestic Patent No. 1588105

It is an object of the present invention to irradiate LED UV-B on the oyster mushroom or oyster mushroom.

In addition, the present invention is to wash and dry mushrooms, sliced or powdered, and then irradiated with LED UV-B for 10 to 15 minutes.

In addition, an object of the present invention is to give a time required for biosynthesis by allowing the mushroom to be left as it is for 1.5 to 3 hours after irradiation with LED UV-B.

In order to achieve the above object, the process of irradiating LED UV-B on mushrooms is listed in order,

① Washing oyster mushrooms or oyster mushrooms in running water for 10 minutes;

② After washing is complete, drying so that the moisture content is 8%~1%;

③ slicing or pulverizing the dried pine mushroom or oyster mushroom;

④ irradiating the powder or sliced mushrooms with LED UV-B for 10 to 15 minutes;

⑤ step of inducing biosynthesis by leaving the mushrooms after the LED UV-B irradiation at a temperature of 5 to 18° C. for 1.5 to 3 hours;

It is in the processing of mushrooms enriched with vitamin D2 by going through.

As a result of irradiating the LED UV-B according to the present invention on the powder of oyster mushroom or oyster mushroom, the synthesis of vitamin D2 was increased compared to the case of irradiation with conventional ultraviolet radiation or LED UV-A or LED UV-C, and vitamin D2 By ingesting the enhanced processed mushrooms of the present invention, in particular, osteoporosis in menopausal women and the elderly was alleviated, the rate of weight gain was reduced, and it was confirmed that it is effective in diabetes, heart disease, and degenerative arthritis.

1 is a processing process diagram of mushrooms.

LED (Light Emitting Diode) is a semiconductor device that emits light by passing an electric current through compounds such as gallium arsenide, and since the development of red LED in the United States in 1968, the color of light varies according to the properties of materials such as yellow, green, blue, and white. In February 2000, Professor Seong-ju Park of Gwangju Institute of Science and Technology succeeded in developing the world's first LED using amorphous silicon quantum dots in April 2001. LEDs using amorphous silicon quantum dots are quantum dots. It is a method of freely adjusting the size of the quantum dot, and has the advantage of freely realizing full-color light if only the size of the quantum dot is adjusted.Also, while the existing lighting has a wide wavelength range, it is accurate in the case of LED. Since it implements a very narrow wavelength range, it is very useful for promoting plant growth or preventing pests. LED UV is divided into UV-C, UV-B, and UV-A according to the wavelength of the ultraviolet rays generated by a high-tech semiconductor light source that artificially emits ultraviolet rays existing in nature, and double UV-A using ultraviolet rays has a wavelength. Although the sterilization power is weak at 320~400nm, it is harmless to the human body, while UV-C has a strong sterilization power with a wavelength of 100~280nm. UV-B is a medium-wavelength ultraviolet ray that is an intermediate region between UV-A and UV-C, and has a wavelength of 280 to 315 nm, and is used for cosmetic suntan, skin disease treatment, medical use, etc. It provides a method of processing mushrooms with increased content of vitamin D2, characterized in that irradiation on oyster mushrooms. In the synthesis of vitamin D by ultraviolet rays, as shown in [Table 1] below, ergocalciferol, vitamin D2, is synthesized when ergosterol, which is a plant sterol, is irradiated with ultraviolet rays.

As for terms used in the present invention, general terms that are currently widely used are selected, and in certain cases, there may be terms arbitrarily selected by the applicant, but this is not a simple term, but a meaning described or used in the detailed description of the invention. The meaning should be grasped in consideration.

The processing method of mushrooms enriched with vitamin D2 using LED UV-B according to the present invention ① Washing oyster mushrooms or oyster mushrooms in running water for 10 minutes; ② After washing is complete, drying so that the moisture content is 8%~1%; ③ slicing or pulverizing the dried pine mushroom or oyster mushroom; ④ irradiating the powder or sliced mushrooms with LED UV-B for 10 to 15 minutes; ⑤ LED UV-B irradiation of the mushrooms are left at a temperature of 5 ~ 18 ℃ 1.5 hours to 3 hours to induce biosynthesis; it is characterized by including.

In step ① above, wash the foreign substances on the prepared mushrooms with running water, and in step ②, dry them using hot air or microweb (MW). At this time, a drying temperature of 55 to 65°C is suitable, and drying is performed for 12 to 15 hours. If the drying temperature is 55°C or less, it takes a long time to dry, and if the drying temperature is 65°C or more, there is a disadvantage that the synthesis rate of vitamin-D is lowered because there is a risk of mushrooms ripening. The mushrooms are sliced or powdered to facilitate the irradiation of LED UV-B on the dried mushrooms. In the case of sliced mushrooms, a thickness of 2 to 3 mm is appropriate, and when powdered, a particle size of 50 to 100 mesh is appropriate, and when the powder is more than a slice, the synthesis rate of vitamin-D is higher. And in the present invention, when the LED UV-B is irradiated and left as it is for 1.5 to 3 hours, the synthesis rate of vitamin-D was found to be high.

Hereinafter, examples of the present invention will be described in detail. However, the following examples are only illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

Experiment method

1. Drying of mushrooms

Each 100 g of white pine and oyster mushrooms before drying were hot-air dried in an oven at 60°C for 12 hours to form dry matter (DM, Dry Matter). As can be seen from Table 2, the moisture content of the fresh oyster mushroom and the fresh oyster mushroom was around 90%, and as a result of drying them at 60°C for 12 hours, the moisture content was around 10%. All of them were irradiated with LED UV-B and general UV-B for 10 to 15 minutes before and after drying.

Dry weight Weight before drying g Weight after drying g moisture % building % Pine mushroom 100 18.6±4.0 89.8±1.4 10.2±1.4 Oyster mushroom 100 33.4±6.2 91.7±0.3 8.3±0.3

2. Measurement of vitamin-D content change according to UV irradiation

First, after irradiating LED UV-B for 0 to 25 minutes on the oyster mushrooms and oyster mushrooms before and after drying in [Table 2], the result of measuring the content of vitamin-D is as shown in [Table 3], and in [Table 2] The results of measuring the content of vitamin-D after irradiation with UV-B for 0 to 25 minutes before drying and after drying are shown in Table 4.

Measurement of changes in vitamin-D content according to LED UV-B irradiation (ug/100g mushroom (wet weight)) Raw mushroom (LED UV-B irradiation) Dried mushrooms (LED UV-B irradiation) 0 minutes 5 minutes 10 minutes 15 minutes 20 minutes 25 minutes 0 minutes 5 minutes 10 minutes 15 minutes 20 minutes 25 minutes Bird 0 0.02 0.05 0.09 0.12 0.25 0.1 1.4 2.5 2.9 3.2 4.4 Oyster 0 0.01 0.04 0.06 0.10 0.11 0.08 1.2 2.2 2.4 2.9 4.0

Measurement of changes in vitamin-D content according to general UV-B irradiation (ug/100g mushroom (wet weight)) Raw mushrooms (normal UV-B irradiation) Dried mushrooms (normal UV-B irradiation) 0 minutes 5 minutes 10 minutes 15 minutes 20 minutes 25 minutes 0 minutes 5 minutes 10 minutes 15 minutes 20 minutes 25 minutes Bird 0 0.00 0.01 0.03 0.09 0.12 0.0 0.5 1.0 1.5 1.9 2.0 Oyster 0 0.00 0.01 0.02 0.08 0.09 0.0 0.4 0.9 1.2 1.4 1.8

As can be seen from the above [Table 3] and [Table 4], compared to the general UV-B irradiation, when the LED UV-B is irradiated, the content of vitamin-D was found to increase, compared to the dry mushroom When the mushrooms were irradiated with LED UV-B, the content of vitamin-D was increased.

3. Osteoporosis experiment using matsutake mushroom irradiated with LED UV-B

Animal experiments were conducted using the extract obtained by extracting the white pine mushrooms irradiated with LED UV-B for 25 minutes with ethanol. In the animals used in the experiment, 21 7-week-old Balb/c female mice (weight 20 g) with ovaries removed were fed diets lacking vitamin-D and calcium to induce osteoporosis. These mice were dissolved in distilled water according to the concentration and administered as shown in [Table 5].

The results of measuring the weight change in each of the samples are shown in [Table 6].

According to <Figure 1>, the weight gain of the negative control (ovarian excision group) increased the most, and the weight gain decreased in the case of the administration group administered with the mushroom extract with increased vitamin-D, thus reducing osteoporosis and resulting in weight gain. It was estimated that the speed was moderate.

<Figure 1> Changes in body weight following administration of mushroom extract

<Figure 2> Bone density measurement by micro CT

According to <Figure 2>, the bone mineral density was the lowest when the negative control (ovarian extraction group) and the mushroom extract without vitamins (CM100) were administered, and the bone density when the mushroom extract with increased vitamin-D was administered. It can be seen that a significant increase in is observed, and osteoporosis is alleviated, and thus an increase in bone density can be confirmed.

<Figure 3> Bone density staining of femur

According to <Figure 3>, when the negative control (ovarian extract group) and the vitamin-free mushroom extract (CM100) were administered, the formation of trabecular bone was microscopic, and osteoporosis was seriously formed, and was the lowest. In the case of the administration group to which the mushroom extract with increased vitamin-D was administered, it was confirmed that the bone mineral density was significantly increased, so that osteoporosis was alleviated and the bone mineral density was increased.

Claims (4)

Washing the oyster mushroom or oyster mushroom in running water for 10 minutes;
After washing is completed, drying the moisture content to be 8% ~ 1%;
Slicing or powdering the dried king matsutake or oyster mushroom;
Irradiating the powder or sliced mushrooms with LED UV-B for 10 to 15 minutes;
Inducing biosynthesis by leaving the mushrooms after the LED UV-B irradiation at a temperature of 5 to 18° C. for 1.5 to 3 hours;
The processing method of mushrooms enriched with vitamin D2, characterized in that consisting of. The method of claim 1,
A method of processing mushrooms enriched with vitamin D2, characterized in that the oyster mushrooms or oyster mushrooms are dried using hot air or microweb (MW). The method according to claim 1 or 2,
A method of processing mushrooms enriched with vitamin D2, characterized in that the oyster mushrooms or oyster mushrooms are dried for 12 to 15 hours at 55 to 65°C. The method of claim 1,
A method of processing mushrooms enriched with vitamin D2, characterized in that powdered oyster mushroom or oyster mushroom.

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