KR20200131786A - Food composition for treating or alleviating edema by promotion of lymphokinesis - Google Patents

Abstract

Disclosed is a food composition for alleviating edema, which promotes lymphokinesis in the body to improve the environment around cells and helps cell metabolism to recover. The food composition for alleviating edema according to the present invention includes Cnidium officinale, maple syrup, Citrus aurantium, Phyllostachys bambusoides, Kalopanax bark and Coix seed in a powder, liquid or extract form.

Description

Food composition for improving edema by promoting lymph circulation {FOOD COMPOSITION FOR TREATING OR ALLEVIATING EDEMA BY PROMOTION OF LYMPHOKINESIS}

The present invention relates to a food composition for improving edema, and more particularly, to a food composition for improving edema that can help improve the environment around cells by promoting lymphatic circulation in the body and restore normal metabolism of cells.

The inventors of the present invention have found that smooth lymph circulation plays an important role in maintaining a healthy body condition and recovering a diseased body quickly through numerous clinical experiences.

Bodily fluid circulation includes both blood circulation and lymph circulation and is responsible for the physical circulation of substances in the body. Blood circulation plays a pivotal role in material circulation in the body, and lymph circulation plays an auxiliary role in helping material circulation in the cardiovascular system.

The lymphatic system is composed of lymph fluid, lymphatic vessels, and lymph nodes. They are responsible for returning excess fluids that may exist between the tissues of the human body back to the heart. In addition, it plays a role of sending waste products left after cell metabolism from tissues and cell metabolites such as fats and proteins with a high molecular weight that cannot pass through capillaries through the vena cava into the blood. Capillary lymphatic vessels exist adjacent to the capillaries and are distributed throughout the body. Tissue fluid in the tissue enters the capillary lymphatic vessels and becomes lymph fluid. When body fluid enters the lymphatic vessel, it does not pass through the membrane, unlike capillary blood vessels, but through small holes at the end of the capillary lymphatic vessel. Therefore, substances having a large molecular weight that cannot pass through the capillary membrane can easily pass through, and most of the components of tissue fluid are included in lymph fluid. The distal portion of the capillary lymphatic vessel has a structure that makes it difficult for the body fluid to escape from the lymphatic vessel. The composition of lymph fluid is similar to that of tissue fluid and is composed of water, dead cells, fat and protein, salt, sugar, urea, and lymphocytes. Lymphatic vessels are the pathways that carry lymph fluid from tissues to the vena cava. Lymphatic vessels are divided into shallow lymphatic vessels located between the skin and fascia and deep lymphatic vessels located below the fascia, depending on their location. Most of them are made up of shallow lymphatic vessels. Therefore, the superficial lymphatic vessel plays an important role in the lymphatic circulation, and it is possible to promote lymphatic circulation through the lymphatic massage that physically stimulates the skin. The movement of lymphatic fluid is mainly caused by a small power generated by a contraction and relaxation movement of the valve and muscle in the lymphatic vessel. When the muscle contracts, the volume inside the lymphatic vessel decreases as the lymphatic vessel is partially pressed by the thickened muscle. At this time, the lymph fluid does not go in the direction blocked by the valve as much as the volume of the lymphatic vessel is reduced, but moves toward the heart. On the contrary, when the muscles are relaxed, the volume inside the lymphatic vessels increases as the lymphatic vessels that were pressed partially become thinner. At this time, lymph fluid must be replenished by the increased volume, but it cannot come from the direction of the heart blocked by the valve, but moves away from the heart to fill it. Therefore, one section of the valve acts as a small heart-like pump that helps lymphatic circulation according to the movement of the muscles. In addition, since a number of these small pumps are connected in series, muscle movements in long sections such as arms and legs act as a power source that strongly promotes the movement of lymph fluid. Capillary lymphatic vessels, which are closer to the skin than capillaries, are susceptible to infection by pathogenic microorganisms such as bacteria and viruses even with light damage to the skin. To compensate for this, lymph nodes are present before the lymphatic vessels reach the vena cava. Lymph nodes have a mesh structure and the internal mesh structure can be well blocked, so they are mainly distributed under the ears, under the tongue, under the armpits, and in the groin, where there is a lot of movement or large muscle movement. Lymph nodes produce lymphocytes to protect the body from invasion by pathogens, and filter, break down and remove pathogens such as bacteria and viruses, and dead cells and wastes in lymph fluid before returning to the blood. The movement of lymph fluid begins when tissue fluid enters the capillary lymphatic vessels. The capillary lymphatic vessels join the lymphatic vessels, and the lymphatic vessels contain lymphatic valves that block the backflow of lymphatic fluid like valves in a vein and make the lymphatic fluid circulate. Again, the lymph fluid passes through the lymph nodes, which are responsible for preventing microbial invasion, and merges with blood in the vena cava. Thus, lymph circulation begins at the tip of the fingertips, toes and head and proceeds toward the heart, then passes through the lymph nodes in the armpits, groin, under the ears and under the tongue to filter pathogenic microorganisms and waste products, merges with the vena cava and enters the vena cava. This is the circulation of body fluids.

As such, the lymphatic system is closely related to the cardiovascular system and is an auxiliary circulatory system that supports fluid circulation. When substances are exchanged between capillaries and tissues, structurally, the amount of body fluid that flows from the capillaries into the tissue is always greater than the amount that enters the capillaries from the tissue. At this time, if the remaining body fluid accumulates as it is, edema occurs in the tissue and the hydrostatic pressure increases. In addition, blood loses moisture due to insufficient body fluids, making it dry and sticky. If these phenomena are not improved and persist, serious fluid circulation disorders are caused, leading to many problems. The lymphatic system plays a role in improving such imbalance by allowing fluids accumulated in tissues to move to the vena cava through lymphatic vessels, which are channels through which fluids can move from tissues to vena cava. Thus, edema in the human body means a disorder of fluid circulation, and a disorder of fluid circulation means a disorder of lymphatic circulation. In addition, whether improving systemic edema or local edema, improving human body edema means improving fluid circulation disorders, and improving fluid circulation disorders means improving lymphatic circulation disorders.

Lymphatic circulation is the process of collecting stagnant body fluids in every corner of the body and returning them to the blood through the vena cava. In the process, it promotes the discharge of waste products and allows the recycling of what is needed. From the perspective of simply bodily fluid circulation, blood circulation is more important than lymph circulation, but from the perspective of tissue environment that affects cell metabolism, lymph circulation, which is directly involved in the circulation of tissue fluid, is more important than blood circulation. Lymphatic circulation first aids blood circulation in the cardiovascular system and promotes the supply of nutrients, moisture, oxygen, hormones, etc., which are necessary substances for cells, and at the same time quickly removes wastes, toxic substances, and inflammatory substances accumulated in tissues or cells. It helps to create an optimal environment for cells to function normally. In addition, it helps the absorption and movement of ingested medicines or nutrients, and increases the effect by quickly delivering them to the required area. As such, lymph circulation plays a large role in creating the optimal conditions for cells to function, so it can be said that it is an important factor for healthy human body activity.

Lymphatic circulation disorder causes systemic swelling or local swelling because body fluid accumulated in tissues cannot return to blood through lymphatic circulation and remains in the tissues. In other words, it can be concluded that both systemic swelling or local edema of the body is a lymphatic circulation disorder. If such edema is short-term, it is not a big problem, but if it lasts for a long time, it lowers the metabolic function of the affected area and acts as a cause of many diseases. Considering that muscle exercise is the only driver of lymph circulation, all symptoms that can occur due to lack of exercise are symptoms of lymphatic circulation disorder, and all the effects that can be improved by exercise are the effects that can be obtained by promoting lymph circulation. Lymphatic circulation gains circulatory power only through muscle exercise, and it is a reality that the modern people, who have less need for movement for survival, lack the opportunity for muscle movement, so that lymphatic circulation is not smoothly performed. Therefore, it is urgent to find a method to promote lymph circulation for modern people who have insufficient body fluid circulation due to absolute lack of exercise. Nevertheless, a lot of discussions have been made about blood circulation and a lot of countermeasures have been developed, but it is true that the discussion of lymphatic circulation is insufficient compared to its importance. The concept of lymphatic circulation has not yet been recognized as medically important compared to its practical importance, and the development of medicines is also insufficient.

It is an object of the present invention to provide a food composition for improving edema that can help improve the environment around cells by promoting lymphatic circulation in the body and restore normal metabolism of cells.

Another object of the present invention is to provide a food composition for improving edema that may occur due to lymphatic circulation disorders.

The above-described problems of the present invention can be solved by a food composition for improving edema, including cinnamon, maple syrup, crust, tabasheer, haedongpi and uiyiin in powder, liquid or extract form.

The food composition for improving edema of the present invention can be made into pills, granules, or powders for oral administration by mixing maple syrup and tabasheer in a mixed powder of cnidium, crust, haedongpi and uiyiin.

The food composition for improving edema of the present invention is a pill for oral administration by mixing maple syrup and tabasheer in a liquid or powdery extract extracted with water, ethanol, or water and ethanol from the mixed powder of Chungoong, Crust, Haedongpi and Uiiin. , Can be made into granules, powders or liquids.

The food composition for improving edema of the present invention includes water, ethanol, or an extract extracted with water and ethanol by mixing cinnamon, maple syrup, crust, tabasheer, haedongpi, and uiyiin at a certain ratio as an active ingredient, and a pill for oral administration , Can be made into granules, powders or liquids.

The food composition for improving edema according to the present invention basically improves the environment around cells by smoothing lymph circulation in the body, helps to restore normal cell metabolism, and allows cells in the body to function normally. It creates the environment. Directly, it promotes lymph circulation in the body and exerts the effect of improving edema.

The promotion of lymphatic circulation according to the present invention basically circulates the body fluids accumulated in the tissues through the lymphatic vessels to merge with blood in the vena cava, and through this, the body fluids are included in the systemic circulation, so the first direct effect is the improvement of edema. Effect. Edema is caused by stagnation of body fluids. The stagnation of body fluids in the human body can be resolved only by lymphatic circulation. Since the circulation of bodily fluid occurs through lymph circulation, stagnation of bodily fluid means a disorder of lymph circulation. Edema manifests as systemic edema or localized edema. Systemic edema can be regarded as a systemic lymphatic circulation disorder, and localized edema can be considered a local lymphatic circulation disorder. Relieving the stagnation of body fluids will eliminate edema. Therefore, it can be directly confirmed that lymphatic circulation is improved from the improvement in edema by the present invention.

1 is a photograph of the product contents of the composition according to the present invention.
2 is a photograph of the product packaging of the composition according to the present invention.
3 is an anatomical view showing the lymphatic vessels of mice used to measure the change in lymph fluid flow rate of the composition according to the present invention.
4 is a chart showing the measurement result of the change in lymph fluid flow rate of the composition according to the present invention.
5 to 8 are diagrams showing the results of the edema improvement test of the composition according to the present invention.

The composition according to the present invention includes cinnamon, maple syrup, crust, tabasheer, haedongpi and uiyiin in the form of powder, liquid or extract.

The composition according to the present invention is composed of the following compositional components and composition ratios.

i) 20-50% by weight of cnidium known to be good for blood circulation (based on the total weight, as follows);

ii) 20-50% by weight of maple syrup that has a sweet taste and is used as a sugar substitute or sweetener, but not known about its pharmacological action;

iii) 10 to 30% by weight of crust known to cause stagnant flags

iv) 10-30% by weight of tabasheer known to lower heat and sputum, and

v) 5-25% by weight of haedongpi, known to pass the meridians well;

vi) 1-20% by weight, which is known to be effective in edema.

If less than 20% by weight of cnidium is added, the treatment and alleviation effect of edema caused by cnidium is insignificant, and when more than 50% by weight of cnidium is added, the treatment and alleviation effect of swelling due to cnidium is increased. Not crazy

If maple syrup is added less than 20% by weight, the treatment and alleviation effect of maple syrup is insignificant. If maple syrup is added in excess of 50% by weight, maple syrup increases the treatment and alleviation effect of edema caused by maple syrup. It does not reach an increase in the amount of added.

If the crust is added less than 10% by weight, the treatment and alleviation effect of crust-induced edema is insignificant, and if the crust is added in excess of 30% by weight, the treatment and alleviation effect of crust-induced edema is increased. Not crazy

If tabasheer is added less than 10% by weight, the treatment and alleviation effect of edema caused by tabasheer is insignificant, and if tabasheer is added in excess of 30% by weight, the increase in the treatment and alleviation effect of edema caused by tabasheer does not reach the increase in the amount of tabasheer added. .

If less than 5% by weight of thawed skin is added, the treatment and alleviation effect of edema caused by thawed skin is insignificant, and if more than 25% by weight of thawed skin is added, the treatment and alleviation effect of edema caused by thawed skin is increased. Not crazy

If less than 1% by weight of Ui-in is added, the treatment and alleviation effect of edema by Ui-in is insignificant, and when more than 20% by weight of Ui-in is added, the treatment and alleviation of edema by Ui-in is increased. Not crazy

The composition according to the present invention can be made into pills, granules, or powders for oral administration by mixing maple syrup and tabasheer in a mixed powder of cnidium, crust, haedongpi, and uiyiin.

The composition according to the present invention is a liquid or powder extract extracted with water, ethanol, or water and ethanol from the mixed powder of Chungoong, Crust, Haedongpi, and Uiiin, by mixing maple syrup and tabasheer for internal use as pills, granules, powders, or It can be made liquid.

The composition according to the present invention includes, as an active ingredient, an extract extracted with water, ethanol, or water and ethanol by mixing in a certain ratio of cinnamon, maple syrup, crust, tabasheer, haedongpi, and uiyiin, as an active ingredient, as an oral pill, granule, powder or It can be made liquid.

Cheongung is a dried rootstock of a parsley family, such as Gunggung or Sangungi. The taste is spicy and the nature is warm.

Maple syrup is concentrated by collecting sap from Canadian maple trees and is widely used as a substitute for sugar.

The crust is a mature fruit of a light-tangerine tree, which is known to cause stagnation to spin and improve indigestion.

Tabasheer, It is a collection of bamboo sap that is evaporated by strong heat in the process of making charcoal from bamboo.

Haedongpi is dried bark of the Ogalpiaceae family.

Uii-in is the dried yeomun seeds of the rice family plant, Yulmu. Yulmu is planted in various places in Korea. After the seeds are ripe in the fall, cut the outpost and pat dry, shake off the seeds, and peel the seeds.

Among them, tabasheer, haedongpi, and maple syrup have the most direct and powerful effect on the treatment and alleviation of edema, regardless of their component ratio. Therefore, if one component of tabasheer, haedongpi, and maple syrup is omitted, the treatment and alleviation effect of edema is remarkably lowered. Cheongung, Jigak, and Ui-in supplement and strengthen the healing and alleviating effects of tabasheer, haedongpi, and maple syrup edema. The composition according to the present invention exerts various effects such as improving edema, improving inflammation and pain, removing bruises, regulating body temperature, improving cellular environment, and promoting drug delivery, because it exerts a synergistic effect by adding crest, perception, and uijin. to be.

Since the promotion of lymphatic circulation is to create an optimal environment for metabolic activity of cells, the composition according to the present invention can be used for almost all symptoms arising from an improper cell metabolic environment. In a narrow range, it can be used not only to improve edema, improve pain, improve inflammation, control body temperature, remove bruises, and promote drug delivery, but if expanded widely, it can be used for all symptoms that can be caused by lack of exercise, which is the cause of lymphatic circulation. have. In addition, the composition according to the present invention can be used in almost all fields for preventing diseases, improving symptoms of diseases, and treating diseases.

It does not cause problems even if it is mixed with drugs or functional foods with other functions, but rather helps the absorption and delivery of active ingredients, and improves the cellular environment of the site causing symptoms, thus increasing the efficacy of the active ingredients. For example, when the composition according to the present invention is taken together with vitamin C or a fat-soluble blood circulation agent, an effect of reliably increasing their effect is obtained.

<Example 1: C4U-1>

In order to confirm the clinical experiment and drug efficacy of the composition according to the present invention, the composition for oral administration was prepared several times in the following amount.

Make a mixed powder of 2,700g of Chungoong powder, 1,400g of crust powder, 1,100g of haedongpi powder, 800g of Uuiin powder, and add 2,600g of maple syrup and 1,400g of tabasheer to make about 10,000g in a small ring with a diameter of 3-5mm. And packaged them in units of 4.8g.

<Example 2: C4U-2>

About 1,000 g of syrup was made by mixing 220 g of thawed blood extract, 500 g of maple syrup, and 280 g of tabasheer, and these were packaged in units of 4.8 g.

<Example 3: C4U-3>

Chungoong extract 540g, crust extract 300g, and Uuiyiin extract 160g were mixed to make about 1,000g of syrup, and these were packaged in units of 4.8g.

[Test for evaluating the change in the flow rate of lymphatic fluid by collecting lymphatic fluid from the rat mesenteric lymphatic vessel]

1) Purpose of the experiment: To evaluate the increase in lymph flow rate of C4U-1, C4U-2, and C4U-3 samples, we established an experimental method to collect lymph from the mesenteric lymphatic vessels of rats, and the effect of each sample on the lymphatic flow rate and This was to evaluate the effect of promoting lymphatic circulation.

2) Experiment method

(1) Animal treatment and sample administration

To check lymphatic vessels, 1.5 ml of peanut oil is administered orally to mice (Male, Sprague-Dawley Rat, body weight 240, 250g) that have been fasted for 24 hours. After 30 minutes of administration of Peanut oil, C4U-1 was administered orally by taking an amount equivalent to 1,666 mg to form a powder, and then dispersing it in a small amount of purified water. C4U-2 and C4U-3 were administered orally by taking 3,636 mg accurately, divided into two. As a control group, purified water in the same amount as C4U-2 and C4U-3 was orally administered. The mice were anesthetized by administering 1 ml of urethane intraperitoneally 30 minutes after the sample administration.

(2) Lymphatic fluid collection and lymphatic fluid flow measurement

As shown in Figure 3, the peritoneum of the anesthetized rat was incised 3-4 cm. At this time, the small intestine was slightly moved to the side of the abdominal cavity and then protected with gauze moistened with warm physiological saline. A fine tube made of polyethylene (0.5 mm inner diameter and 0.9 mm outer diameter) was inserted into a white and shiny lymphatic vessel (see FIG. 3) located vertically in the vena cava under the liver, and lymphatic fluid was collected for 1 hour. After 1 hour, the microtube was removed, and the weight of the flow rate of lymph fluid flowing into the microtube was measured with a microbalance.

3) Experiment result

The test results are shown graphically in FIG. 4. As can be seen with reference to Figure 4, The flow rate of the lymph fluid collected for 1 hour without administration to the rat was 1.1 mg by weight, and there was little change in the flow rate of the lymph fluid even when measured after administration of purified water. However, after administration of C4U-1, C4U-2, and C4U-3, the flow rate increased significantly to 1.8 mg (63.6% increase), 1.6 mg (45.4% increase), and 1.3 mg (18.2% increase). It was found that samples 1, C4U-2, and C4U-3 can significantly increase the flow rate of lymph fluid. This change in flow rate was noticeable even during lymphatic collection. Through this, it can be seen that when C4U-1, C4U-2, and C4U-3 are administered, lymphatic circulation is promoted. In particular, it can be seen that the increase rate of lymphatic circulation promotion of C4U-2 is more than twice that of C4U-3, and the increase rate of lymphatic circulation promotion of C4U-1 is more than three times of that of C4U-3. Able to know. That is, it was confirmed that the extracts of Haedongpi, Maple syrup, and tabasheer had a greater effect on promoting lymphatic circulation than the extracts of Chungoong, Jigak and Uiiin, and when they were all mixed, the effect of promoting lymphatic circulation was synergistic.

[Carrageenan-induced foot edema test using Sprague-Dawley rat]

1) Test purpose

The easiest way to check the effect of promoting lymph circulation is the edema test. This is because almost all conditions that cause edema involve stagnation of lymph fluid. Therefore, it is possible to check the lymphatic circulation effect as a method to check the improvement of edema.

2) Test substance

C4U-1, C4U-2, and C4U-3 were used as test materials, and Diclofenac Sodium was used as a positive control material. As excipient 1, sterile water for injection (Daehan sterile distilled water-source: Daehan Pharmaceutical Industries Co., Ltd.) was used, and as excipient 2, 0.9% saline (sterilized physiological saline solution-source: Daehan Pharmaceutical Industries, Ltd.) was used.

3) Test method

(1) Test preparation

Specific pathogen-free (SPF) rats, Hsd:Sprague Dawley®™ SD®™ (producer and source: Coretech Co., Ltd. (181-21, Jinwi-ro, Jinwi-myeon, Pyeongtaek-si, Gyeonggi-do), male 6 weeks old, 30 males were used. Rats were chosen because they are widely used in various drug efficacy and toxicity tests, and abundant test data are accumulated, and it is easy to interpret and evaluate test results using these data. , During the administration and observation period (red), an individual identification card was attached to the breeding box, and an animal room usage record was attached to the entrance of the breeding room. 3 ℃, relative humidity 55±15%, ventilation frequency 10-20 times/hr, lighting time 12 hours (8 am to 8 pm off) and illuminance 150 to 300 Lux. During the breeding period, environmental conditions, such as temperature/humidity, ventilation frequency, and illuminance, etc. in the animal room were measured regularly, as for feed, DreamBio (Gwangnaru, Gwangjin-gu, Seoul, Korea) was used as feed for experimental animals produced by Cargill Agripurina. Rho 507) and freely ingested water, using a polycarbonate drinking water bottle, and a wooden rug supplied from Coretech Co., Ltd. (406 Dongcheon-ri, Jinwi-myeon, Pyeongtaek-si, Gyeonggi-do). During the period of acclimatization and experimentation, no more than 5 animals/carriers were raised in a polycarbonate breeding box for rodents (W 235 x L 380 x H 175 mm), and the breeding boxes, rugs, and water bottles were exchanged at least once a week. The weights of animals determined to be healthy were measured and grouped by a random method so that the average weight of each group was distributed as uniformly as possible according to the ranked weight.

(2) Composition of test group

The composition and dosage of the test group were determined as follows. Test substance 1 represents C4U-1, test substance 2 represents C4U-2, and test substance 3 represents C4U-3. As described above, the positive control material is Diclofenac Sodium.

(3) Preparation of test substance for administration

In the case of Carrageenan, after weighing the required amount, it was prepared by dissolving it at a concentration of 1% (10mg/mL) in sterile physiological saline as an excipient, and used as an edema inducing substance.

In the case of a test substance, at the request of the client, weigh the required amount according to the following information provided by the client, and add sterile water for injection, prepare it to a concentration of 400 mg (based on the main ingredient)/10 mL, and use it for the test. I did.

Test substance 1 (C4U-1)

Formulation: Pill

Prescription: 240 mg of active ingredient in 1000 mg of pills, 760 mg of excipient

Dosage: 400 mpk as main ingredient (about 1666 mg as powder)

Test substance 2 (C4U-2)

Formulation: Syrup

Prescription: 110 mg of active ingredient in 1000 mg of syrup, 890 mg of excipient

Dosage: 400 mpk as the main ingredient (about 3636 mg as a syrup)

Test substance 3 (C4U-3)

Formulation: Syrup

Prescription: 110 mg of active ingredient in 1000 mg of syrup, 890 mg of excipient

Dosage: 400 mpk as the main ingredient (about 3636 mg as a syrup)

In the case of a positive control substance, the required amount was taken, and then prepared to a concentration of 30 mg/10 mL by adding sterile water for injection, and used for the test.

(4) administration

In the case of the test substance, oral administration, which is the intended clinical route, was selected. In the case of Carrageenan, an edema inducing substance, intradermal administration to the sole of the foot was selected. In the case of the test substance, a single dose was administered 2 hours before the administration of the trigger substance (Carrageenan), and the trigger substance was also administered once. For the test substance and the positive control substance, calculate and administer 10 mL/kg based on the body weight measured on the day of the test. In the case of Carrageenan, 50 μL was administered intradermally to the sole of the right hind leg. In the case of oral administration, animals were fixed by the cervical skin fixation method, and administered directly into the stomach using a sonde for oral administration. In the case of Carrageenan, 50 μL was administered intradermally to the sole of the right hind leg. Animals for administration of the test substance were fasted for 16 hours or more before administration of the test substance.

(5) Observation and inspection items

*Before carrageenan administration and 3 hours after injection, measure the volume of the hind limb with a leg edema meter.

It was measured using, and the edema inhibition (%) was calculated using the calculation formula presented below.

* The edema rate (%) and edema inhibition (%) of each test group were calculated.

Edema rate (%) = {(Vt-Vn)/Vn} X 100

Vt: foot volume after a certain time after injection of Carrageenan,

Vn: Foot volume before Carrageenan injection

Edema inhibition (%) = (average edema rate of inducing control group. Edema rate of each individual) / (edema rate of inducing control group) x 100

4) Test result

The test results are shown graphically in FIGS. 5 to 8.

5 is a result of measuring the volume of the hind limb using a lower extremity swelling meter before injection of the test substance and administration of Carrageenan. It can be seen that the volume is about 1 mL in all groups (G1-G6). 6 is a result of measuring the volume of the hind limb 3 hours after administration of Carrageenan to the G2-G6 group using a lower limb edema meter, and FIG. 7 is a difference between the measurement result of FIG. 6 and the measurement result of FIG. 5 (edema Increase volume). FIG. 8 is a table showing the results of calculating the edema rate using the measured value of FIG. 7 and calculating the edema suppressing power (%) using the edema rate.

As can be seen in FIG. 8, it can be seen that the edema inhibitory power of G4(C4U-1) is close to 17%, which is the edema inhibitory power of Diclofenac Sodium as a positive control material. It can be seen that the edema suppressing power of G5(C4U-2) was around 8%, and that of G6(C4U-3) was around 4%. It can be seen that C4U-1, in which the components of C4U-2 and C4U-3 are combined, exhibits a synergistic effect of improving edema. In addition, the lymph promoting effect of the present invention can be confirmed through the effect of improving edema.

[Viscosity Evaluation of Lymphatic Fluid Collected from Mesenteric Lymphatic Vessels in Rats]

1) When C4U-1, C4U-2, and C4U-3 were administered, it was expected that there would be a change in the viscosity of the lymph fluid, and the viscosity of the lymph fluid collected after administration of the sample to the rat was evaluated.

2. Experiment method

1) Animal treatment and sample administration

(1) To 40 rats (Sprague-Dawley rat, male, weight 190-200g) that had been fasted for 24 hours, TPA (12-O-tetradecanoylphorbol-13-acetate) was used in the 4 points of the ears and lower extremities of the rat. Caused skin irritation.

(2) 6 hours after the induction of inflammation, 40 rats were divided into 4 groups of A, B, C, D by 10, and then powdered for groups B, C, and D excluding group A (control group). C4U-1, C4U-2, C4U-3 1,666 mg each was dispersed in purified water (4.5 ml) and administered orally.

(4) 30 minutes after the administration of C4U-1, C4U-2, C4U-3 as above, the rat was anesthetized with chloroform, and 1 ml of urethane was then administered intraperitoneally to maintain the anesthesia of the rat during the experiment. I did.

2) Lymphatic collection

(1) After anesthetizing the rat, the peritoneum of the rat was incised 3-4 cm. After that, the small intestine was placed outside the abdominal cavity and covered with gauze soaked in warm physiological saline to prevent drying.

(2) Insert a polyethylene microtube (ID 0.5 mm, OD 0.9 mm) into a lymphatic vessel located vertically in the vena cava under the liver, and extract the lymph fluid leaking out of the tube into each group A, B, C, D. I collected not much.

3) Measurement of the viscosity of lymph fluid

(1) 2,000 μl of lymph fluid was taken for each group, and a rheometer (model name: HAAKE RheoStress 1, Thermo Fisher Scientific Co. Ltd.) equipped with a 35 mm diameter parallel plate (P TiL 30, Thermo Fisher Scientific Co. Ltd., USA). , USA) was used to measure the viscosity of the lymph fluid. At this time, the gap between the two plates was set to 1.0 mm and the temperature of the lower plate was set to 20℃.

(3) After putting about 2,000 μl of the sample between the two plates, the sample was stabilized for 5 minutes.

(4) The strain value of the sample was measured while applying a shear stress of 0.1 Pa for 500 seconds.

Measured.

(5) After the measurement was completed, the zero shear viscosity was calculated.

* 3. Experiment results

As shown in FIG. 11, in the case of the control group (group A), the zero shear viscosity was measured to be 2.21 mPa·s on average, and C4U-1 (group B), C4U-1 (group C), and C4U-3 (group D). ), the zero shear viscosity averaged 1.78 mPa·s (about 19.5% decrease in viscosity), 1.85 mPa·s (about 16.3% decrease in viscosity), and 1.93 mPa·s (about 12.7% decrease in viscosity). ) Was measured.

[Clinical test]

Hereinafter, unless otherwise described, the composition according to the present invention is C4U-1 according to Example 1.

<Measurement of edema improvement effect>

Edema refers to a condition in which fluid such as lymph fluid or tissue exudate is accumulated in tissue due to lymphatic circulation disorders and excessively exists. When swelling occurs in the skin and soft tissues, it swells clinically and has a loose feeling, and when pressed, the skin temporarily dents. When lymphatic circulation is smooth, these symptoms disappear. Therefore, improvement of edema means improvement of lymphatic circulation. When edema improvement is confirmed, it can be concluded that there is an improvement in lymphatic circulation.

50 female patients who are 50 years of age or older with edema of the lower extremities were selected, and these patients were asked to take the composition according to the present invention twice a day, two capsules at a time, and after taking 7 consecutive days, each patient They were asked to measure i) change in weight (using a weight scale), ii) change in circumference length of the area with the most edema among the thighs, calves, and insteps (using a plastic tape measure), and iii) edema status (visual observation). The results are shown in Table 3 below.

From the measurement results below, it was confirmed that the circumferential length of the most severe part of lower extremity edema was decreased in 45 (90%) of 50 patients, and weight was decreased in 40 (80%) patients. And, it was confirmed that the edema condition was alleviated in 46 patients (92%). It was confirmed that the circumference of the edema area decreased in the range of 1.0% to 10.0% of the circumference before administration. It was confirmed that the body weight decreased in the range of 0.5% to 4.0% of the body weight before administration. Body fluids (lymphatic or tissue exudate) stagnated in the lower extremity tissues are excreted into the stool through urine or bile, resulting in weight change. Improvements in swelling are usually accompanied by significant weight loss.

From these results, it can be confirmed that the edema of the present invention is improved, that is, the lymphatic circulation is improved.

Measurement item

Number of patients (persons)
Patient rate


Changes in the circumference of the thigh, calves, or instep where the swelling is most severe
The circumference of the edema area has decreased.

45
90%
There is no change in the circumference of the edema

4
8%

The circumference of the edema area has increased.

One
2%



Weight change
I lost weight.

40
80%
No change in weight

9
18%
Gained weight

One
2%



Edema condition
The edema condition has been alleviated.

46
92%
No change in edema condition

4
8%
The edema condition worsened.

0
0%

<Clinical example for individual patients>

The following clinical trial results are, of course, physiologically predicted results when lymph circulation is promoted, and are confirmed through clinical practice.

1) Clinical trial 1-Improvement of edema (43 years old, female)

After surgery for thyroid cancer, the patient continued to take the thyroid drug Synthroid for a long period of time and had symptoms of swelling of the whole body as well as the face and fingers every morning due to weak poisoning. It was observed with the naked eye that the swelling of the face disappeared after 7 days and the swelling of the whole body disappeared after 5 days as the composition according to the present invention began to be eaten as b.i.d. It could be confirmed that the weight was also reduced by 2.0 kg. The patient himself stated that his body became lighter, his face became smaller, and his skin was improved. Treatment of edema generally limits salt intake or administering diuretics, but does not produce satisfactory effects. According to the present invention, it was confirmed that a very rapid edema improvement effect appeared. Since edema occurs due to stagnation of body fluids, and stagnation of body fluids is caused by lymphatic circulation disorders, the improvement in edema means that the lymphatic circulation is improved.

2) Clinical trial 2-Improvement of edema (54 years old, female)

This patient was a 54-year-old female pharmacist with frequent allergies and swelling. Due to degenerative arthritis, there was always swelling in the knee. I was bitten by a mosquito a few days ago, but I left it. After two days, my wrist became swollen and itchy. When I applied the steroid ointment, it spread largely, swelling and fever. Two packets of the composition according to the present invention were taken, and two packets were again taken after 5 hours. The next morning, little swelling of the wrist was observed. After that, 1 packet of b.i.d was given for 5 more days, and the swelling of the knee was no longer observed. Since edema occurs due to stagnation of body fluids, and stagnation of body fluids is caused by lymphatic circulation disorders, the improvement in edema means that the lymphatic circulation is improved.

3) Clinical trial 3-Improvement of edema (40 years old, female)

A 40-year-old female patient who was overweight and complained of frequent swelling of the insteps and soles of the feet and pain in the back neck and shoulders. The composition according to the present invention was taken for 5 days in t.i.d. Five days after taking the drug, the swelling of the back and soles completely disappeared, and he stated that he felt a little less weight. It was determined that a large amount of systemic waste products were discharged by promoting lymphatic circulation.

4) Clinical trial 4-Improvement of edema (72 years old, female)

It was a female patient with signs of cracked skin due to swelling of the lower extremities for more than 10 years. My skin was sensitive, so I felt pain even when I touched my hand slightly. Entelon Tab (150mg), marketed as a circulatory drug, was taken for several years, but there was no improvement. This patient was allowed to take the composition according to the present invention for 20 days at a time t.i.d. After 20 days, it was visually confirmed that the edema was reduced to the extent that wrinkles appeared on the calf skin.

<Manufacturing and marketing>

The composition according to the present invention was prepared in the following composition ratios and packaging units.

A mixed powder of 27% by weight of Chungoong powder, 14% by weight of crust powder, 11% by weight of defrosted skin powder, and 8% by weight of Uuiin powder was prepared. After making into a freeze-dried ring, it was packaged in a stick of 4.8 g.

1 is a photograph of the product contents of the composition according to the present invention, and FIG. 2 is a photograph of the product packaging of the composition according to the present invention.

From May 1, 2017 to March 31, 2018, more than 6,000,000 stick packages (4.8g per stick package) were sold through 2,000 pharmacies in Korea. As a result of tracking the results of administration to 2,000 edema patients through pharmacists participating in the sales, 1,960 (about 98%) patients had a weight loss effect of 1.0% to 5.0% along with edema relief.

Claims (1)

A food composition for improving swelling by promoting lymphatic circulation, characterized in that it contains cinnamon, maple syrup, crust, tabasheer, haedongpi and uiyiin in powder, liquid or extract form.

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