KR20200115811A - A method of detecting a sample change using a light source control method, the light source control method, and a sample change detection device - Google Patents

Abstract

Disclosed is a sample change detection device. An objective of the present invention is to provide equipment which can replace an expensive spectrometer conventionally used in color and brightness detection. The sample change detection device comprises: a light source to emit light of various wavelength bands; a sample unit which is positioned on the path of the light by the light source, and allows a sample to be arranged thereon; a light detection unit to detect light penetrating the sample unit in various wavelength bands; a light source control unit to receive signal information detected through the light detection unit to adjust the strength of the light applied to the sample unit and applied by the light source; and an optical signal calculation unit to determine an optical change of the sample arranged on the sample unit based on a detection result where the light of which the strength is controlled by the light source control unit penetrates the sample unit. The light source control unit can adjust the strength of the light emitted from the light source to allow the light to have the same output value in various wavelength bands in the light detection unit for the sample of the sample unit.

Description

A method of controlling a light source, a method of detecting a sample change using a method of controlling a light source, and a sample change detection device {A METHOD OF DETECTING A SAMPLE CHANGE USING A LIGHT SOURCE CONTROL METHOD, THE LIGHT SOURCE CONTROL METHOD, AND A SAMPLE CHANGE DETECTION DEVICE}

The present invention relates to a method for controlling a light source, a method for detecting a change in a sample by using the method for controlling a light source, and an apparatus for detecting sample change.

In hospitals, for qualitative/quantitative detection of specific pathogens in body fluids, samples that change color depending on the presence or absence of pathogens or samples whose brightness changes according to the concentration of pathogens are used. In the chemical field or bio field, which has been recently studied, there are many cases of research using changes in color or brightness as a result of the reaction of a sample. Whether or not the color of the sample has changed is now mostly visually checked, which is less accurate. In addition, if the color is similar, it is difficult to see with the naked eye. To verify whether the color or brightness of the sample has changed, most of the ELISA reader or spectrometer equipment is used. However, these devices are large, heavy, and expensive, and because they check and analyze the measurement results using a computer, it is difficult to apply them to real-time diagnosis in a medical or laboratory field. That is, in the existing technology, there was a need to measure the change in color and brightness of a sample, but there was a problem in which it was inaccurate to check with the naked eye and inefficient to measure it with an equipment.

In the present invention, it is intended to provide an equipment capable of replacing an expensive spectrometer used for detecting changes in color and brightness.

In addition, in the present invention, it is not limited to the type of sample, and an object of the present invention is to provide a device capable of detecting an optical change of a sample.

The problem to be solved by the present invention is not limited to the problems mentioned above. Other technical problems that are not mentioned will be clearly understood by those of ordinary skill in the art from the following description.

A device capable of detecting a change in a sample is disclosed.

The sample change detection device may include a light source emitting light of various wavelength bands; A sample unit positioned on a path of light by the light source and in which a sample is disposed; A light detector configured to detect light transmitted through the sample part in various wavelength bands; A light source control unit configured to receive signal information detected through the light detection unit and adjust the intensity of light emitted from the light source applied to the sample unit; And an optical signal calculating unit configured to determine an optical change of the sample disposed in the sample unit based on a detection result of the light whose intensity has been controlled through the light source control unit transmitted through the sample unit.

When the light source control unit adjusts the intensity of light applied to the sample unit, a sample or a reference sample is disposed in the sample unit before the chemical reaction, and the sample unit when the optical signal calculating unit determines the optical change of the sample is Any sample for which an optical change is desired may be placed.

The arbitrary sample may be the sample during the chemical reaction or the sample after the chemical reaction.

The light source control unit may adjust the intensity of the light to have the same output value in various wavelength bands of the light detection unit with respect to the light of each wavelength band emitted from the light source and transmitted through the sample unit.

The light detector may detect the light transmitted through the sample part using an optical sensor capable of measuring in a wavelength band corresponding to a color included in the light source.

The light emits light of at least three colors, and the light source control unit may adjust the intensity at which each light of at least three colors is emitted.

The optical signal calculator includes at least three colors included in the light source in a sample during or after the reaction based on the output of a sample before reaction or a reference sample to which controlled light to have the same output value from the photo detector is applied. It is possible to compare the result of the change in the output of the sensor in each wavelength band corresponding to the above light.

The optical signal calculator may determine whether or not an optical characteristic is changed through a reaction of the sample from the output result of the sensor.

The optical signal calculator may determine a real-time optical characteristic change during the reaction of the sample from the output result of the sensor.

The change in optical properties appearing from the output result of the sensor may be any one of a change in color of the sample, a change in brightness of the sample, or a change in real time of the sample.

The sample change detection device may further include a display configured to output a change analysis result of the optical characteristic of the sample.

According to another embodiment of the present invention, a method of controlling a light source for detecting a change due to a chemical reaction of a sample is disclosed.

A method for controlling a light source for detecting a change due to a chemical reaction of a sample, the method comprising: detecting light emitted from an initial uncontrolled light source through the sample to be detected in the form of an electric signal by a light detector; Analyzing the detected signal and controlling the light by feeding back the detected signal so that the detection result for each wavelength band of the light transmitted through the sample has the same value.

The control of the intensity of each color included in the light source may be independently processed.

The step of feeding back and controlling the light so that the detection result for each wavelength band of the light transmitted through the sample has the same value may be repeatedly performed until the same output value appears.

According to another embodiment of the present invention, a method of detecting a change in a sample using the method of controlling a light source is disclosed.

If the output value of the sample has a constant output value in each wavelength band included in the light source, irradiating the controlled light to the sample during or after the reaction to derive an output value in each wavelength band; It is possible to detect a sample change including.

A sample change, characterized in that it is possible to detect a change in a sample, characterized in that it is determined whether or not an optical characteristic of the sample is changed through a portion different from the constant output value by comparing the constant output value and the output value after the reaction in a wavelength band.

By comparing the constant output value and the output value during the reaction in a wavelength band, a change in the optical characteristic during the reaction of the sample in real time may be determined through a portion different from the constant output value.

According to the present invention, it is possible to provide an equipment that can replace the spectrometer used for detecting changes in color and brightness.

According to another effect of the present invention, it is economical because it can be miniaturized at low cost while having a similar level of detection accuracy compared to a spectrometer used for a similar purpose.

According to another effect of the present invention, since the types of samples that can be detected are not limited, equipment applicable to various fields may be provided.

According to another effect of the present invention, not only before and after the reaction of the sample, but also the optical change of the sample during the reaction can be detected.

The effect of the present invention is not limited to the above-described effects. Effects not mentioned will be clearly understood by those of ordinary skill in the art from the present specification and the accompanying drawings.

1 is a block diagram schematically illustrating a sample change detection apparatus according to the present invention.
2 is a diagram showing an embodiment of a sample change detection device according to the present invention.
3 is a diagram for explaining an algorithm in the sample change detection apparatus according to the present invention.
4(a) to 4(c) are diagrams showing an example of a sample before reaction and a result value of a sample after reaction according to the sample change detection method according to the present invention.
5 and 6 are diagrams for explaining an example when a sample change detection device according to the present invention is used.
7 is a flowchart illustrating a sample change detection method according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art can easily implement the embodiments of the present invention. However, the present invention may be implemented in various different forms and is not limited to the embodiments described herein. In addition, in describing a preferred embodiment of the present invention in detail, when it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted. In addition, the same reference numerals are used throughout the drawings for portions having similar functions and functions.

"Including" a certain component means that other components may be further included, rather than excluding other components unless specifically stated to the contrary. Specifically, terms such as "comprises" or "have" are intended to designate the presence of features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, but one or more other features or It is to be understood that the presence or addition of numbers, steps, actions, components, parts or combinations thereof does not preclude the possibility of preliminary exclusion.

Terms such as first and second may be used to describe various components, but the components should not be limited by the terms. These terms are used only for the purpose of distinguishing one component from another component. For example, without departing from the scope of the present invention, a first element may be referred to as a second element, and similarly, a second element may be referred to as a first element.

Singular expressions include plural expressions unless the context clearly indicates otherwise. In addition, shapes and sizes of elements in the drawings may be exaggerated for clearer explanation.

The'~ unit' and'~ module' used throughout this specification are units that process at least one function or operation, and may mean hardware components such as software, FPGA, or ASIC. However,'~ unit' and'~ module' are not meant to be limited to software or hardware. The'~ unit' and the'~ module' may be configured to be in an addressable storage medium, or may be configured to reproduce one or more processors.

As an example,'~ unit' and'~ module' are components such as software components, object-oriented software components, class components and task components, processes, functions, properties, Procedures, subroutines, segments of program code, drivers, firmware, microcode, circuits, data, databases, data structures, tables, arrays, and variables. Components and functions provided by'~ unit' and'~ module' may be performed separately by a plurality of elements and'~ unit' and'~ module', or may be integrated with other additional components. .

The present invention provides a device capable of easily detecting a change before, after or during a reaction of a sample using a light source capable of adjusting color and a sensor capable of measuring colors included in the corresponding light source. The device is a device capable of detecting a change in a sample. Based on each output from the optical sensor detected by the sample before reaction or the reference sample passing through the light source, the sample after the reaction or during the reaction is detected through the light source. Through comparison with each output of the optical sensor, it is possible to easily detect the optical change pattern of the sample by analyzing the change in the output of the optical sensor in various wavelength bands.

In the present invention, a light source controller capable of controlling a light source and a light source capable of adjusting color are used to detect a change according to a reaction regardless of the type of sample. In addition, by controlling the light source through feedback using the detected result, it is possible to check the light detection result of the sample in real time, thereby improving the stability and accuracy of the result. The sample change detection device according to the present invention can be miniaturized at low cost while having a detection accuracy similar to that of a spectrometer used for a similar purpose. It is expected to be useful for real-time diagnosis in a medical field. In addition, the types of samples that can be detected are not limited, and detection is possible regardless of the wavelength band, and thus it is expected to be applicable to various fields as chemical sensors and biosensors.

The detectable range of the present invention is not limited to the visible light region, and can be applied to samples in which absorbance changes are detected in the infrared and ultraviolet regions. In the light source and the light detection unit of the present invention, the light source includes an infrared region or an ultraviolet region other than a visible ray region, and an optical sensor capable of detecting light in the region is used to prevent a human eye from seeing. It may also be possible to detect changes in the sample at.

As an example, the light source of the present invention may include at least three colors in a visible light region, an infrared light source, and an ultraviolet light source. As an example, the light detection unit of the present invention may include an optical sensor capable of detecting the at least three colors in a visible light region, an infrared detection sensor and an ultraviolet detection sensor.

Hereinafter, for convenience of explanation, a light detection unit including a light source emitting light in the visible light region and an optical sensor detectable in the visible light region will be described. However, the present invention is not limited to this description.

What will be described below is only an embodiment of the present invention, and is not interpreted as being limited to the parts disclosed in the drawings and description of the invention. Hereinafter, a technical concept and an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

1 is a block diagram schematically illustrating a sample change detection apparatus according to the present invention.

The sample change detection device 1 according to the present invention includes a light source control unit 11 and a light source 10, a sample unit 20, a light detection unit 30, an optical signal calculation unit 40, and a display unit 50. can do.

The light source 10 may be a light source capable of emitting three or more colors. The light source 10 may emit light of various wavelength bands. At least three colors included in the light source 10 according to an embodiment of the present invention may be red, green, and blue. According to an embodiment, when red, green, and blue are output from a light source, these are colors corresponding to the three primary colors of light, and when the three colors are combined with the same intensity, white light is obtained. In addition, if each ratio is finely adjusted, most color combinations that humans can perceive with the eye are possible. By selecting the three primary colors of light from the three colors that can be output by the light source to react with the sample in all wavelength bands, a sample change detection device with general versatility can be provided. However, this is only an example, and the three colors included in the light source may be selected as three colors among colors in the visible light wavelength range. The light source may include four or more colors. The light source 10 of the present invention is capable of emitting light of various colors by combining at least three types of optical signals. The light source 10 of the present invention may additionally include an infrared light source and an ultraviolet light source in at least three types of visible light regions.

The light source 10 may be provided in the form of a light source capable of eliciting multicolored light from one. Alternatively, the light source 10 may be provided in the form of at least three monochromatic lights each capable of deriving one color. When a plurality of light sources 10 are provided, a distance between each light source and a sample included in the sample unit 20 may be provided by being spaced apart by the same distance.

In the sample part 20, a sample may be fixed. The sample part 20 may be located on an optical path of light generated by the light source 10. The sample fixed to the sample part 20 may be a sample before a chemical reaction. Alternatively, the sample fixed to the sample unit 20 may be a sample after a chemical reaction. The sample fixed to the sample part 20 may be a sample during a chemical reaction. Alternatively, the sample fixed to the sample unit 20 may be a reference sample.

The reference sample means a sample having the same optical properties as the sample before the chemical reaction. If the reference sample and the sample before the chemical reaction are subjected to a chemical reaction in the same way, a chemical reaction occurs in the sample before the chemical reaction, but the reference sample does not undergo a chemical reaction and maintains the same optical properties as the sample before the chemical reaction. That is, the reference sample is a sample used as a reference.

According to an embodiment, some of the samples may not exhibit optical changes after a chemical reaction. This sample is a sample showing the same optical properties as the sample before the chemical reaction even when the chemical reaction proceeds and is completed. Such a case may occur because the concentration of the reactant contained in the sample is lower than a degree to cause a change in optical properties, or the reaction proceeds under a temperature condition different from the temperature condition in which an optimal chemical reaction can occur.

The sample fixed to the sample unit 20 may be a sample having a colored type. Or it may be an intangible sample. The sample unit 20 may further include a fixing device for fixing the sample so that the position of the colored or intangible sample does not change the optical path during the measurement process. Light emitted from the light source 10 may pass through the sample unit 20 and pass through the sample fixed to the sample unit. The sample unit 20 may be located between the path of the light source 10 and the light detection unit 30.

The light detection unit 30 may include a sensor capable of simultaneously measuring at least three or more colors. The optical sensor included in the optical detection unit 30 may be a digital sensor. This allows the output to be expressed as a unitless number. Referring to FIG. 4, it can be seen that the output is output without a unit. However, depending on the type of optical sensor, a sensor that outputs an analog signal such as voltage or current may also be used.

According to an aspect of the present invention, the light detection unit 30 may be started to enable detection in a corresponding wavelength region by including the number of colors of light output from the light source 10 and the number of sensors corresponding to the number of colors. The optical sensor can detect and detect at least three color optical signals simultaneously. Accordingly, the optical sensor included in the optical detection unit 30 can measure at all wavelengths within the visible light band. A photo detector or a photo diode may be used as an optical sensor included in the photo detector 30. As described above, the light detection unit 30 may further include a sensor capable of detecting an infrared region and an ultraviolet region even outside the visible region. The light detection unit 30 may detect an output value of light at a wavelength corresponding to each light source and transmit it to the light source control unit 11 or the optical signal calculation unit 40.

The light source controller 11 is connected to the light source 10 and may control the intensity of light having at least three or more respective colors included in the light source. The light source control unit 11 may adjust the intensity of light emitted from the light source 10 based on the detection signal detected by the light detection unit 30. The light source control unit 11 may adjust the intensity of each color of the light source 10 so that the output detected by each optical sensor of the light detection unit 30 has the same value. When the light source control unit 11 adjusts the intensity of light applied to the sample unit 20, a sample before a chemical reaction may be disposed in the sample unit or a reference sample may be disposed. It can be used as a reference value before reaction by controlling the intensity of light based on a reference sample or a sample before a chemical reaction.

The optical signal calculating unit 40 may determine whether or not the sample is optically changed by using the detection result of the sample before reaction and the sample after reaction. The optical signal calculation unit 40 is based on the detection result of the light controlled to have the same value as the output detected by the light detection unit 30 through the light source control unit 11 through the sample after the reaction or the sample during the reaction. It is possible to determine whether the optical change of The optical signal calculating unit 40 may further include a circuit unit for outputting and calculating a detection result of the sample. The optical signal calculating unit 40 may analyze the optical change of the sample by measuring the sample before the reaction and comparing the measurement results of the sample after the reaction. The optical signal calculating unit 40 may analyze real-time color and brightness changes during the reaction.

When the optical signal calculating unit 40 determines the optical change of the sample, an arbitrary sample for which the optical change is to be determined may be disposed in the sample unit 20. The arbitrary sample may be a sample during a chemical reaction or a sample after a chemical reaction. That is, the optical signal calculator 40 may derive an optical change pattern of the sample by comparing the output of the sample during the reaction or the sample after the chemical reaction based on the output of the sample before the chemical reaction or the reference sample.

The display unit 50 may output a result calculated by the optical signal calculating unit 40. The output result of the display unit 50 may be a result of a color change of the sample. The output result of the display unit 50 may be a result of a change in brightness of the sample. The output result of the display unit 50 may be a result of a change in absorbance of the sample. The output result of the display unit 50 may be a time taken until the reaction of the sample is completed. Alternatively, whether the brightness and color of the sample changes from the start of the reaction can be output in the form of a graph. The user of the sample change detection device 1 may check whether the sample is changed by viewing the result displayed on the display unit 50.

2 is a diagram showing an embodiment of a sample change detection device according to the present invention.

According to FIG. 2, the sample change detection device 1 may include a certain platform. The platform may be a platform capable of maintaining a constant optical path. The platform may be located on the sample unit 20. The platform may maintain a constant optical path during a sample change detection process.

The sample change detection device 1 according to an exemplary embodiment of the present invention may be provided in a closed configuration. The inner surface of the sample change detection device 1 may be formed of a material capable of minimizing a loss that occurs while the light from the light source 10 travels along a path. The outer surface of the sample change detection device 1 may be formed of a material for preventing the external optical signal from being transmitted into the sample change detection device 1. That is, when the light source 10 is irradiated to the sample, the sample change detection device 1 may not be affected by any external influence, and may be formed by treating the surface so as to increase accuracy by preventing light loss from inside. The sample change detection device 1 may be configured to maintain a constant optical path and block noise signals in the vicinity. The sample change detection device 1 can create an optimal light path condition and a dark room environment.

According to the embodiment of FIG. 2, the detection result of the light detection unit 30 may be fed back through the light source control unit 11. In the light source control unit 11, the intensity of each light emitted from the light source 10 can be independently adjusted. As an example, it is assumed that each color included in the light source 10 is red, green, and blue, and the light source controller 11 applies the same intensity to each color included in each light source. At this time, the three colors will be combined with the same intensity and output as white light. When the intensity applied to each color is different, the output color may be variously changed according to fine adjustment. However, even if the intensity is the same and the output is the same, when the corresponding light is transmitted through the sample, the characteristics of the output light may vary according to the characteristics of the sample. In the present invention, the intensity of each light applied to the light source 10 through the light source control unit 11 is controlled to display the same output for the sample before the reaction or the reference sample. By detecting the difference in change, it is possible to easily detect the change in the sample.

For example, when light is emitted with the same intensity of each light source, white light is output, but if it is incident on the sample before reaction, the color or brightness is likely to change. In this state, if the initial light is incident on the sample after the reaction without controlling the light source, the color or brightness changes again when the characteristics of the sample change, making it difficult to notice the change on the graph. However, in the present invention, by controlling the light source control unit 11 through feedback from the light detection unit 30 so that the same output value, for example, the output of white light appears with respect to the sample before reaction or the reference sample, a reference value is set and changes are easily made. There are features that can be detected. That is, it is easy to check and compare the degree of change in each output of the sample after the reaction compared to the sample before reaction. In addition, when light is first incident on a sample, in the process of controlling the light so that the same output according to the reaction of the sample after setting all of the light intensity constant, there is an effect of confirming the characteristics of the sample before the reaction. .

The optical signal calculation unit 40 matches each output by the sample before the reaction through the light source control unit 11 equally, and then measures the output of the optical sensor in real time while proceeding the reaction of the sample, and it takes until the reaction is completed. It is possible to analyze the optical change pattern of the sample over time, such as whether the time, color, and brightness change steadily from the beginning to the end of the reaction or rapidly change after a certain period of time.

A detailed sequence according to the sample change detection method in the present invention is shown in FIG. 3. 3 is a diagram for explaining an algorithm in the sample change detection apparatus according to the present invention. Contents for describing the sample change detection method in the present invention as a relationship between the light source control unit 11 and the light source 10, the sample unit 20, the light detection unit 30, and the optical signal operation unit 40 are disclosed. have.

The sample change detection method according to the present invention may be processed in the following order. The sample before reaction or the reference sample is fixed to the sample part 20. Initial light is incident on the fixed sample. The initial light at this time may be a light source in which the intensity of each color included in the light source is set to the same value. Before adjusting the intensity of each color of the light source 10, the reason for setting the intensity of each light source 10 equally is when the intensity of each color of the light source is adjusted to the reference value after measuring the sample before reaction. This is to easily grasp how much each has changed compared to the initial period. During control by the light source control unit 11, if the intensity of each of the individual colors of the light source 10 increases, the absorbance decreases in the band near the corresponding wavelength, and if the intensity decreases, the absorbance in the band near the wavelength. It can be seen as an increase. That is, when the intensity of each light source is adjusted to be the same and then the process of controlling the light source so that the reference value is output, it is possible to know about what kind of absorbance distribution the sample before reaction has in the visible light wavelength band.

At this time, since the intensity of each color of the light source after pre-measurement will increase or decrease in the process of being adjusted to the reference value, the intensity of each color of the initial light source can be freely changed. It may be desirable to set it around the middle of the maximum intensity that is present.

The light detection unit 30 detects a signal in which the initial light reacts by the sample or reference sample before reaction, and transmits the detected signal to the light source control unit 11 in the form of an electric signal. The light source control unit 11 may adjust the intensity of each light included in the light source 10 so that the detection result for each wavelength by the light detection unit 30 for the initial light transmitted through the sample before the reaction or the reference sample is the same. . As a result of adjusting the intensity of light through the light source control unit 11, it is determined whether the output value of the signal detected by the light detection unit 30 is the same value. By analyzing the output result of the optical sensor of the light detection unit 30, it is determined whether the output value after applying the initial light to the sample before reaction or the reference sample is the same as the value set as the reference. When controlling the light source control unit 11 to output a constant reference value, it is easy to check and compare the degree of change of each output of the sensor of the sample after the reaction compared to the sample before the reaction. In this case, the reference value may be a specific value of the output of the optical sensor of the optical detection unit 30. The specific value may be an intermediate value between a maximum value and a minimum value that the sensor output can have. There is no limit to the value that can be the reference value, but some of the following points should be considered. In the process of adjusting the intensity of each light source so that each output of the sensor becomes the same value after pre-measurement, the intensity of any one of at least three colors included in the light source becomes too weak or too strong. The measurement result of the sample after the reaction may be unreliable. Therefore, it is not desirable to set too specific reference values.

The photodetector 30 compares the output values of each optical sensor included in the photodetector 30 with each other, and generates a signal to control the light source so that the samples before reaction have the same values as measured by each optical sensor. You can give feedback. In this case, the light source control unit 11 may independently adjust the intensity of each color included in the light source 10. When the above-described process is repeated and the output values from each optical sensor detected by the light detection unit 30 are all the same, the light to which the adjusted intensity is applied is regarded as controlled light.

The sample after the reaction or the sample during the reaction is fixed to the sample unit 20 to store the value of the signal output from the light detection unit 30, and the optical signal calculating unit 40 stores a reference value that is a result of the sample before reaction or the reference sample, The optical change of the sample is determined by comparing the results of the sample after the reaction or the sample during the reaction. Result processing for the sample before reaction and the sample after reaction may be measured repeatedly under the same conditions by controlled light.

That is, according to FIG. 3, the light source control unit can control the light source by receiving feedback from the result from the light detection unit, so that a more accurate result value can be detected.

4 is a diagram illustrating an example of a sample before reaction and a result value of a sample after reaction according to the sample change detection method according to the present invention. The results shown in FIG. 4 mean the average value of the sensor output, and the lines shown up and down mean the standard deviation of the sensor output measured repeatedly. The Y-axis of the graph means the output value of the digital sensor. Units are expressed as ADUs (Analog-to-Digital Units).

Fig. 4(a) shows the results when the controlled light is applied to the sample before the reaction. As described above, since the light source control unit 11 has the characteristic that the intensity of each color is adjusted so that the same value is output for the sample before reaction, the result value when the controlled light is applied to the sample before reaction is It can be measured to have the same value in the band. According to FIG. 4A, it can be seen that the output value by the optical sensor is derived as 100 in all areas of blue, green, and red. In this case, it corresponds to the reference value set to 100.

Fig. 4(b) shows the results when the controlled light is applied to the sample after the reaction. According to the output graph, it can be seen that the output value by the optical sensor is about 90 in blue, about 110 in green, and about 87 in red. According to the above change, it can be seen that the output value of green is increased compared to the reference value of 100, and the output of red and blue is decreased compared to the reference value of 100. Through the above results, it means that the color of the sample after the reaction has changed so that light having a wavelength band near green is better transmitted and less light having a wavelength band near red and blue is transmitted compared to the sample before reaction. do. That is, it can be seen that the absorbance of the sample after the reaction decreases in the wavelength band near green compared to the sample before the reaction, and the absorbance increases in the red and blue wavelength bands. From these results, it can be seen that the color of the sample was changed through a chemical reaction.

According to an embodiment, when controlled light is applied to the sample after the reaction, a result value as shown in FIG. 4(c) may appear. In the case of Fig. 4(c), the output value is constant, but the absolute value of the output value is changed. In this case, it means a change to better transmit light in all wavelength bands of red, blue, and green. That is, it can be seen that the absorbance of the sample after reaction decreased in all visible wavelength bands compared to the sample before reaction. From these results, it can be seen that the brightness of the sample has changed through the chemical half, and it can be seen that the sample has changed brighter from the change in absorbance in each of the wavelength bands.

That is, when the sample change detection device according to the present invention is used, the change in absorbance in a specific wavelength band within various wavelength bands can be measured in real time as the reaction of the sample proceeds and the component or concentration of the sample is changed, By using this, you can simply grasp the color change, brightness change, degree and trend of the sample.

5 and 6 are diagrams for explaining an example when a sample change detection device according to the present invention is used.

5 is a diagram of a sample before a chemical reaction, and FIG. 6 is a diagram of a sample after a chemical reaction. In FIGS. 5 and 6, a wavelength of approximately 450 nm is regarded as a blue output wavelength band of the optical sensor, and 550 nm and 650 nm are considered as a green and red output wavelength band of the optical sensor. 5(a) is a result of measuring a sample before the reaction using a spectrometer. 5(b) is a result of measuring a sample before reaction using the sample change detection device 1 according to the present invention. As a result of measuring the absorbance of the sample before reaction, the absorbance of blue and red is relatively high, and the absorbance of green is relatively low. In other words, as a result of the pre-measurement of the sensor for the sample before reaction, the blue and red light sensor output is low, and the green light sensor output is high. 5(c) is an output of the sample change detection device 1 that appears as a result of adjusting each color of a light source so that the blue, red, and green outputs of the optical sensor are all the same. The reference value at this time was set to 100.

6(a) is a result of measuring a sample after reaction using a spectrometer. 6(b) is a result of measuring a sample after the reaction using the sample change detection device 1 according to the present invention. As a result of measuring the absorbance of the sample after the reaction, the absorbance of blue and green decreased, and the absorbance of red was increased compared to the sample before reaction. Therefore, as a result of the measurement of the sample after the reaction, it can be seen that the output of the optical sensor was measured higher than the reference value for blue and green, and the output of the optical sensor was measured lower than the reference value for red.

That is, when using an existing spectrometer, the same results as in FIGS. 5(a) and 6(a) appear, and optical changes can be known through analysis of the graph, but since this is expensive, the sample change detection of the present invention By deriving the same results as in Figs. 5(b) and 6(b) through the device 1, there is an effect that the optical change of the sample can be easily detected. Even when compared with the results using the spectrometer of FIGS. 5(a) and 6(a), the same results are shown, and there is an effect that can be used economically and less reaction time compared to the spectrometer.

7 is a flowchart illustrating a sample change detection method according to the present invention.

With respect to the pre-reaction sample, an uncontrolled initial light source passes through the pre-reaction sample or the reference sample. In this case, by matching the intensity of each color included in the light source to be the same, the output of the sample before reaction or the reference sample may be measured in advance. Through the feedback of the measured signal, the light source control unit adjusts the intensity of each color so that the output value of the sample before reaction or the reference sample has the same value. (S20) At this time, it is checked whether each output value from each optical sensor is output as the same value. (S30) At this time, if the output value is not the same, the corresponding value is fed back again, and this process is repeated until the same result is obtained. If the output values are the same, the intensity of the light is set to the controlled light. Thereafter, controlled light is incident on the sample after the reaction or the sample during the reaction to detect the reaction. (S40) Since the result of the controlled light incident on the sample before the reaction or the reference sample will be the same output value as above, based on this, the output value of the light detection unit 30 for the sample after the reaction or the sample during the reaction is compared. By doing so, it is possible to determine whether or not the optical change of the sample or the change of optical properties of the sample in real time (S50)

The sample change detection device according to the present invention can detect changes in color and brightness according to changes in components and concentrations of a sample regardless of the type of sample, such as ions, polymers, and DNA. In addition, it is possible to check the change in absorbance in a specific wavelength band in real time, regardless of the type of sample, as well as the color of the sample before and after the reaction, and through what process. In addition, changes in color and intensity of fluorescence of a fluorescent sample can be detected according to the condition setting. When the sample change detection apparatus and method according to the present invention is used, it can be applied to various fields as a chemical sensor and a bio sensor that is inexpensive, has high accuracy, and has portability.

It should be understood that the above embodiments have been presented to aid the understanding of the present invention, and do not limit the scope of the present invention, and various deformable embodiments are also within the scope of the present invention. The technical protection scope of the present invention should be determined by the technical idea of the claims, and the technical protection scope of the present invention is not limited to the literal description of the claims itself, but a scope that has substantially equal technical value. It should be understood that it extends to the invention of.

Sample change detection device: 1
Light source: 10
Light source control unit: 11
Sample part: 20
Light detection unit: 30
Optical signal operation unit: 40
Display: 50

Claims (17)

A light source emitting light of various wavelength bands;
A sample unit positioned on a path of light by the light source and in which a sample is disposed;
A light detector configured to detect light transmitted through the sample part in various wavelength bands;
A light source control unit configured to receive signal information detected through the light detection unit and adjust the intensity of light emitted from the light source applied to the sample unit; And
And an optical signal calculating unit configured to determine an optical change of the sample disposed in the sample unit based on a detection result of the light whose intensity is controlled through the light source control unit transmitted through the sample unit.
The method of claim 1,
When the light source control unit adjusts the intensity of light applied to the sample unit, a sample before a chemical reaction or a reference sample is disposed in the sample unit,
A sample change detection device in which an arbitrary sample for which an optical change is to be determined is disposed in the sample unit when the optical signal calculating unit determines the optical change of the sample.
The method of claim 2,
Any of the above samples
A sample change detection device which is the sample during a chemical reaction or the sample after a chemical reaction.
The method according to any one of claims 1 to 3,
The light source control unit
With respect to the light for each wavelength detected by the light detection unit after being emitted from the light source and passing through the sample unit,
A sample change detection device that adjusts the intensity of the light so that the light detection unit has the same output value in various wavelength bands.
The method of claim 4,
The light detection unit,
A sample change detection device that detects light that has passed through the sample part by using an optical sensor that can be measured in a wavelength band corresponding to a color included in the light source.
The method of claim 5,
The light emits at least 3 colors or more,
The light source control unit is a sample change detection device for adjusting the intensity of each light emitted from the light source at least three colors or more.
The method of claim 6,
The optical signal calculation unit,
Each corresponding to at least three colors of light included in the light source in the sample during or after the reaction, based on the output of the pre-reaction sample or the reference sample to which the controlled light to have the same output value from the light detection unit is applied A sample change detection device that compares the change results of the sensor output in the wavelength band of.
The method of claim 7,
The optical signal calculation unit,
A sample change detection device that determines whether or not an optical characteristic is changed through a reaction of the sample from the output result of the sensor.
The method of claim 7,
The optical signal calculation unit,
A sample change detection device that determines a change in optical characteristics in real time during a reaction of the sample from the output result of the sensor.
The method of claim 7,
The change in optical characteristics appearing from the output result of the sensor is any one of a change in color of the sample, a change in brightness of the sample, or a change in real time of the sample.
The method of claim 8,
The sample change detection device,
A sample change detection device further comprising a; display unit for outputting a change analysis result of the optical characteristic of the sample.
In the method of controlling a light source for detecting a change due to a chemical reaction of a sample,
Detecting the light emitted from the uncontrolled initial light source through the sample in the form of an electric signal by the light detection unit;
And controlling the light by analyzing the detected signal and feeding back the detected signal so that the detection result for each wavelength band of the light transmitted through the sample has the same value.
The method of claim 12,
The light source control method, characterized in that the control of the intensity of each color included in the light source is independently processed.
The method of claim 12,
The controlling of the light by feeding back the light so that the detection result for each wavelength band of the light transmitted through the sample has the same value is repeatedly performed until the same output value is displayed.
In the method of detecting a sample change using any one of the light source control method of claim 12 to 14,
If the output value of the sample has a constant output value in each wavelength band included in the light source, irradiating the controlled light to the sample during or after the reaction to derive an output value in each wavelength band; A method of detecting a change in a sample comprising a.
The method of claim 15,
Comparing the constant output value and the output value after the reaction in a wavelength band, and determining whether or not an optical characteristic of the sample is changed through a portion different from the constant output value.
The method of claim 15,
The method of detecting a sample change, characterized in that by comparing the constant output value and the output value during reaction in a wavelength band, and determining real-time optical property change during the reaction of the sample through a portion different from the constant output value.

Images