KR20200103718A - 인간 피부 섬유아세포의 분리 방법 - Google Patents
인간 피부 섬유아세포의 분리 방법 Download PDFInfo
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- KR20200103718A KR20200103718A KR1020207019897A KR20207019897A KR20200103718A KR 20200103718 A KR20200103718 A KR 20200103718A KR 1020207019897 A KR1020207019897 A KR 1020207019897A KR 20207019897 A KR20207019897 A KR 20207019897A KR 20200103718 A KR20200103718 A KR 20200103718A
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- fibroblasts
- human skin
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- cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0656—Adult fibroblasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6881—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from skin
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/415—Wnt; Frizzeled
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Rheumatology (AREA)
- Epidemiology (AREA)
- Developmental Biology & Embryology (AREA)
- Dermatology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1721738.1A GB201721738D0 (en) | 2017-12-22 | 2017-12-22 | Method |
| GB1721738.1 | 2017-12-22 | ||
| PCT/GB2018/053760 WO2019122931A1 (en) | 2017-12-22 | 2018-12-21 | Method for isolating human dermal fibroblasts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20200103718A true KR20200103718A (ko) | 2020-09-02 |
Family
ID=61131714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020207019897A Ceased KR20200103718A (ko) | 2017-12-22 | 2018-12-21 | 인간 피부 섬유아세포의 분리 방법 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US20210095252A1 (https=) |
| EP (1) | EP3728565A1 (https=) |
| JP (2) | JP7475685B2 (https=) |
| KR (1) | KR20200103718A (https=) |
| GB (1) | GB201721738D0 (https=) |
| WO (1) | WO2019122931A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230074037A (ko) * | 2021-11-18 | 2023-05-26 | 의료법인 성광의료재단 | 세포 노화 바이오 마커 syne2 또는 hmgb2, 및 이를 이용한 세포 노화를 검출하는 방법 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB202104130D0 (en) * | 2021-03-24 | 2021-05-05 | King S College London | Method |
| CN115058388A (zh) * | 2022-03-14 | 2022-09-16 | 福建医科大学附属协和医院 | 间充质干细胞创面修复优势功能亚群及其鉴定和应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2801979B1 (fr) | 1999-12-03 | 2002-02-08 | Oreal | Utilisation d'un anticorps specifique des fibroblastes papillaires comme marqueur de qualite d'une peau |
| US9074190B2 (en) | 2003-10-07 | 2015-07-07 | Biomaster, Inc. | Cell differentiation of adipose-derived precursor cells |
| US20070065415A1 (en) * | 2005-09-16 | 2007-03-22 | Kleinsek Donald A | Compositions and methods for the augmentation and repair of defects in tissue |
| JP6042904B2 (ja) | 2011-11-25 | 2016-12-14 | シャネル パフュームズ ビューテ | 乳頭状および網状の線維芽細胞の新規マーカーおよびその使用 |
-
2017
- 2017-12-22 GB GBGB1721738.1A patent/GB201721738D0/en not_active Ceased
-
2018
- 2018-12-21 EP EP18829964.8A patent/EP3728565A1/en active Pending
- 2018-12-21 JP JP2020534824A patent/JP7475685B2/ja active Active
- 2018-12-21 KR KR1020207019897A patent/KR20200103718A/ko not_active Ceased
- 2018-12-21 US US16/956,500 patent/US20210095252A1/en not_active Abandoned
- 2018-12-21 WO PCT/GB2018/053760 patent/WO2019122931A1/en not_active Ceased
-
2023
- 2023-10-19 US US18/490,714 patent/US20240279609A1/en active Pending
- 2023-12-26 JP JP2023218733A patent/JP2024038101A/ja active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230074037A (ko) * | 2021-11-18 | 2023-05-26 | 의료법인 성광의료재단 | 세포 노화 바이오 마커 syne2 또는 hmgb2, 및 이를 이용한 세포 노화를 검출하는 방법 |
| KR20230074030A (ko) * | 2021-11-18 | 2023-05-26 | 의료법인 성광의료재단 | 세포 노화 바이오 마커 mmp15, acsl5 또는 cd36, 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR20230074038A (ko) * | 2021-11-18 | 2023-05-26 | 의료법인 성광의료재단 | 세포 노화 바이오 마커 cdca7l 또는 wdr76, 및 이를 이용한 세포 노화를 검출하는 방법 |
| KR20230074029A (ko) * | 2021-11-18 | 2023-05-26 | 의료법인 성광의료재단 | 세포 노화 바이오 마커 cdca7l, wdr76 또는 h2bc8, 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR20230074027A (ko) * | 2021-11-18 | 2023-05-26 | 의료법인 성광의료재단 | 세포 노화 바이오 마커 ezh2, tmpo 또는 amot, 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR20230074028A (ko) * | 2021-11-18 | 2023-05-26 | 의료법인 성광의료재단 | 세포 노화 바이오 마커 gas2l3, syne2 또는 hmgb2, 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR20230074035A (ko) * | 2021-11-18 | 2023-05-26 | 의료법인 성광의료재단 | 세포 노화 바이오 마커 ezh2 또는 tmpo, 및 이를 이용한 세포 노화를 검출하는 방법 |
| KR20230074040A (ko) * | 2021-11-18 | 2023-05-26 | 의료법인 성광의료재단 | 세포 노화 바이오 마커 acsl5 또는 cd36, 및 이를 이용한 세포 노화를 검출하는 방법 |
| KR20230074031A (ko) * | 2021-11-18 | 2023-05-26 | 의료법인 성광의료재단 | 세포 노화 바이오 마커 pld1, cyp26b1 또는 gsap, 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019122931A1 (en) | 2019-06-27 |
| GB201721738D0 (en) | 2018-02-07 |
| JP2021507714A (ja) | 2021-02-25 |
| US20240279609A1 (en) | 2024-08-22 |
| JP2024038101A (ja) | 2024-03-19 |
| EP3728565A1 (en) | 2020-10-28 |
| JP7475685B2 (ja) | 2024-04-30 |
| US20210095252A1 (en) | 2021-04-01 |
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