KR20200100305A - Growing method of Portulaca oleracea improved selenium and zinc content - Google Patents

Abstract

Provided is a method of cultivating Portulaca oleracea having increased contents selenium and zinc, which comprises the steps of: preparing a mixed solution by mixing ground selenium and zinc with a brown rice vinegar solution and heating the mixture thereof; mixing the mixed solution with alkaline water and stirring the mixture thereof to ionize selenium and zinc; cooling the ionized mixed solution at room temperature to stabilize the same; mixing the stabilized mixed solution with water to dilute the mixed solution so that an activating solution is liquefied; and applying the liquefied activating solution to Portulaca oleracea. Accordingly, the Portulaca oleracea cultivated according to the present invention has increased contents of selenium and zinc, and thus has improved functionality as an antioxidant agent, an anticancer agent, and an immune enhancing agent, thereby promoting the consumption of the Portulaca oleracea and increasing incomes of farmers who cultivate the Portulaca oleracea.

Description

Growing method of Portulaca oleracea improved selenium and zinc content}

The present invention relates to a purslane cultivation method, and more particularly, to a purslane cultivation method enriched with selenium and zinc components having various physiological activities.

Purslane (Portulaca oleracea L) is a one-year plant, also referred to as five-leaf, Jang-myeong-chae, and machichae, and is an edible, fleshy plant commonly found in roadsides, vegetable gardens, and empty lots. It is distributed all over the world, used in salads in the West, and dried in boiling water in the summer and seasoned with vegetables in winter.

In folk remedies, it has been used as an antidote, antiseptic, anti-scurvy, antispasmodic, diuretic, anthelmintic, and skin soothing agent (Journal of the Korean Society of Medicinal Crop Science, Vol. 8 No. 3, p189, 2000; Korean Journal of Food and Nutrition, Vol. 28, No. 3, p607, 1999), and other muscle relaxation activities, antibacterial, anti-cancer and antioxidant effects have also been reported (Journal of the Korean Culinary Research Society, Vol. 17 No. 6, p565, 2001).

As components of purslane, L-noradrenaline, dopamine, dopa and various organic acids, amino acids, and terpene glycosides (portuloside A) are known, and it has been reported that the content of omega-3 fatty acids such as gamma-linolenic acid is high (Korean organisms Journal of the Korean Society of Engineering, Vol. 18, No. 3, p165, 2003) Purslane with these ingredients and efficacy grows naturally in domestic gardens and hills, but the method to create high value-added by utilizing them is still insufficient. It is an urgent need to develop new processed products that can be utilized.

There are many patent documents using purslane known so far, but in particular, “purslane beverage with anticancer and antibacterial effects” in Korean Patent Application No. 10-1999-0025609, and “Pursade in Korean Patent Application No. 10-1999-0019384” Purslane extract with anti-cancer function, purslane extract having anti-tumor activity, “Chinese herbal medicine extract with anti-tumor activity, and purslane extract with free radical scavenging ability and antioxidant activity” of Korean Patent Application No. 10-1998-0000092 The possibility of using it as an anticancer agent can be seen with the composition”.

1. “Purslane drink with anticancer and antibacterial effect” of Korean Patent Application No. 10-1999-0025609 2. Korean Patent Application No. 10-1999-0019384 "Psoriasis extract with anticancer function" 3. “A composition containing purslane extract having free radical scavenging ability and antioxidant activity” of Korean Patent Application No. 10-2003-0022668

However, purslane or purslane extract has not been commercialized as an anticancer agent. This can be solved by providing purslane with an increased content of minerals, particularly selenium and zinc, which have excellent anticancer activity and physiological activity.

therefore. The technical problem to be achieved by the present invention is to provide a method for cultivating purslane in which selenium and zinc components are enhanced.

In order to achieve the above technical problem, the present invention

Preparing a mixed solution by mixing and heating selenium and zinc in a pulverized state in a brown rice vinegar solution;

Ionizing selenium and zinc by mixing and stirring alkaline water in the mixed solution;

Cooling and stabilizing the ionized mixture at room temperature;

Liquefying the active liquid by diluting the stabilized mixture with water; And

It provides a method of cultivation of purslane enriched with selenium and zinc, characterized in that it comprises the step of fertilizing the liquefied active liquid with purslane.

Purslane cultivated according to the present invention as described above, as the content of selenium and zinc increases, improves its functionality as an antioxidant, anticancer agent, and immune enhancing agent, thereby promoting consumption of purslane, thereby increasing the income of purslane farmers.

Detailed contents for carrying out the present invention as described above will be described.

Purslane has excellent regeneration and fertility so that it can be replaced four times a year. When the environment is difficult, self-fertilization is carried out with a closed pollen receiver, and even if the roots are taken, the attached seeds do not stop growing. Due to its fleshy nature, it is resistant to drying and survives for several days even if it is picked in midsummer. It is regarded as a troublesome weed in farming because of its active reproduction. Especially for sesame seeds, allelopathy is strong.

Breeding is done either by giving up or by seeds. It grows well in soils that are mostly watery, manure, and close to acid. It likes sunlight, so growth is sluggish in shady places. It takes approximately 6-8 weeks from sowing to harvest. For early harvesting, plant a hotbed in early spring and sow. In the open field, it can be sown from late spring to summer. Even if the leaves and stems are cut off, new stems continue to emerge, so they can be harvested in summer.

During the cultivation period of purslane, fertilization is performed on purslane. In the present invention,

Preparing a mixed solution by mixing and heating selenium and zinc in a pulverized state in a brown rice vinegar solution;

Ionizing selenium and zinc by mixing and stirring alkaline water in the mixed solution;

Cooling and stabilizing the ionized mixture at room temperature;

Liquefying the active liquid by diluting the stabilized mixture with water; And

It is characterized in that the liquefied active liquid is fertilized with purslane to cultivate purslane enriched with selenium and zinc components.

Specifically, the cultivation method according to the present invention as described above includes preparing an active liquid containing selenium and zinc components, liquefying the active liquid, and fertilizing the liquefied active liquid with purslane.

The preparing of the active liquid may include preparing a mixed liquid by mixing and heating the pulverized selenium and zinc in a brown rice vinegar liquid; Ionizing selenium and zinc by mixing and stirring alkaline water in the mixed solution; And further mixing alkaline water with the ionized liquid mixture and cooling at room temperature to stabilize.

The step of preparing the mixed solution is a step of mixing and heating the pulverized selenium and zinc in a brown rice vinegar solution, and 20 g of each of the pulverized selenium and zinc in a powder state is added to 500 ml of brown rice vinegar solution and mixed at 100° C. for 10 minutes or more. This is a step of preparing a mixed solution by heating.

The ionizing step is a step of mixing and stirring 20 L of alkaline water in 500 ml of the mixed solution, and the stabilizing step is a step of cooling the ionized mixed solution at room temperature to stabilize selenium and zinc in an ionic state.

On the other hand, the step of liquefying the active liquid is a step of obtaining a liquid ratio by mixing the active liquid with water having a neutral pH and diluting it by 1000 times.

That is, by mixing the active liquid stabilized at room temperature with water, it can be sprayed on the purslane in liquid fertilizer, and herbicides or insect repellents are sprayed in preparation for weed damage, pests, and meteorological disasters that may occur during the growth process of purslane. It is preferable to mix and spray the active liquid according to the invention with herbicides and insect repellents so that comprehensive control can be achieved.

In addition, the step of fertilizing the liquefied active liquid to purslane is not particularly limited, but it is preferable to apply the fertilization by repeatedly spraying 3 to 5 times during the growth process of purslane, and purslane is fertilized after a period of nutrient growth It enters the reproductive growth phase in which young ears differentiate and grow, and the liquefied active liquid is fertilized from the reproductive growth phase and fertilized, and the fertilized ovary develops and the storage material is accumulated in the endosperm by foliar spraying at least 3 to 5 times. It is desirable to fertilize.

Purslane cultivated according to the present invention can have the following effects by increasing the content of selenium and zinc.

Selenium belongs to the oxygen and sulfur family of atomic number 34 and is an essential trace element indispensable to humans and animals. Until World War II, selenium had excellent efficacy, but it was also highly toxic and was recognized only as a toxic substance. In 1957, Dr. Schwarz's'Experiment to prevent liver cirrhosis in rats' led to the growth and reproduction of selenium in humans and animals. The discovery that it is an essential nutrient brought a revolutionary turning point in the perception of selenium.

Then, in 1973, Professor Hoekstra, University of Wisconsin, USA and Germany

In a study by Dr. Flohe's team, the discovery that selenium acts as an antioxidant makes selenium a second-generation.

In recent years, by administering selenium to humans, cancer mortality is reduced by about 50%, and that it has a preventive effect of 37% regardless of all cancer diseases, and is particularly effective in preventing prostate cancer, colon cancer, and lung cancer. The discovery by the Clark team of the University of Korea was another big opportunity, and in recent years, selenium has become an object of worldwide interest, regardless of the academic world or the general public.

The antioxidant activity of selenium is mentioned as a preventive role for selenium disease. Like other vitamins, selenium cannot act as an antioxidant on its own, but it is known that it acts as an antioxidant through selenoprotein or complements and synergizes when administered with vitamin E.

Selenium acts as an antioxidant in the form of selenocysteine. For example, thioredoxin is not only important for the oxidation and reduction of cells, but thioredoxin reductase is another method that synthesizes and regulates DNA by reducing nucleotides during DNA synthesis. It is a fact that it plays an important role. In particular, in the case of P and W in selenoproteins, P represents the prevention of arteriosclerosis and antioxidant activity in plasma and endothelial cells (vascular endothelial cells), and W represents glutathione peroxidase (GPX) to act as an antioxidant on muscle. It is known. Reactive oxygen species (hydrogen peroxide, superoxide anion, hydroxy radicals) occurring in our body are produced by metabolic processes, especially mitochondrial electron transport, antibacterial action, anti-viral, and inflammation, and are produced by aging, cancer, arthritis, diabetes, dementia, and cells. Since it provides a cause of death, it can be said that the role of GPX, one of various antioxidant enzymes, which is a defense mechanism of such active oxygen, is very important. In 1973, Professor Hoechstra of the University of Wisconsin in the U.S. and Ph. It turned out to be.

Selenium's cancer prevention action and immune function enhancement are mentioned. Selenium's cancer-preventing action and immune function-enhancing action are quite different from those of the aforementioned antioxidant action. In other words, when looking at the results of various studies so far, it is known that selenium directly penetrates into cancer cells to kill cancer cells, and directly acts on immune function enhancement when it acts to prevent cancer.

Epidemiologic studies and preclinical studies that inhibit the incidence and growth of cancer have demonstrated that selenium in higher amounts than nutritional standards is effective. A recent study by Clark et al. in the United States shows groundbreaking potential. This is the first double-blind, placebo-controlled experiment designed to test the hypothesis that selenium intake can reduce cancer incidence, and 200 grams of selenium per day is consumed. Those who ate had a 50% lower cancer mortality rate, 37% lower cancer rates, 63% lower prostate cancer rates, 58% lower colon cancer rates, and 46% lower rates of lung cancer. That is, as a result of ingesting 200 g of selenium for 4 and a half years, the mortality and incidence rate related to cancer decreased by 40%.

In addition, an experiment to reveal the relationship between the incidence of prostate cancer and selenium intake was conducted in 34,000 men from the Harvard-Based Health Professionals' Cohort Study. Those with lower selenium intakes had a three-fold or more incidence of prostate cancer. Appeared to be high.

Zinc (Zn) has been recognized as an essential trace element of life since the early 1900s, and deficiency was first recognized in the early 1960s. Zinc is an essential trace mineral for various functions in the body, such as cell growth, maturation of reproductive function, and immunity, and is contained in our body about 1.5 to 2.5 g, of which about 60% is distributed in muscles and the rest is distributed in the skeleton.

Zinc affects the action of insulin, which regulates the influx of glucose into fat cells. It is also related to the activity of hormones such as growth hormone, sex hormone, thyroid hormone, and prolactin. Zinc is also involved in immune function.

Zinc is a component of enzymes and is involved in the synthesis and degradation of carbohydrates, proteins, lipids, and nucleic acids. In particular, it is involved in the synthesis of nucleic acid DNA and RNA, and plays an essential role in the process of cell differentiation, proliferation and gene expression, so that growth, tissue and skeleton formation, reproduction, and immune functions are performed smoothly.

Zinc stabilizes the structure of cell membrane proteins, certain hormones, and gene transcription factors. Cell membrane stability affects receptors, and cell membrane function is very important because these receptors signal all kinds of reactions in the cell.

Zinc plays an important role in stabilizing antioxidant enzymes [copper(Cu), zinc-superoxide dismutase(Zn-SOD)] and is also related to sugar metabolism and insulin action. However, it has been reported that zinc supplementation worsens glucose tolerance and does not affect LDL cholesterol oxidation in type 2 diabetes patients, but it has been reported that zinc supplementation reduces LDL cholesterol oxidation by improving zinc deficiency in type 1 diabetes.

Zinc binds to proteins such as albumin and circulates in the blood, and 30-40% of zinc is exchanged in the liver from portal blood.

Zinc has a low absorption rate in grain-based diets, but is high in meat-based diets. In relation to the estimation of zinc utilization, the World Health Organization (WHO) has three types of meals: one with a high zinc utilization rate of 50-55%, a meal with a moderate 30-35%, and a meal with a low utilization of 15%. Classified as. The general diet in Korea is regarded as a meal with a normal use rate (30%).

A meal with a high zinc utilization rate refers to a refined meal with a large amount of meat intake and a small intake of milled grains, and a meal with a moderate utilization rate is a mixed meal that includes meat and fish and does not depend entirely on unmilled grains. A meal with low zinc utilization refers to a diet mainly based on plant foods that rely mainly on unpolished, non-fermented grain foods.

Zinc induces the synthesis of metallothionein, a protein that binds zinc in the same way ferritin binds iron when absorbed in the small intestine. In addition, oxalate and phytate inhibit zinc absorption because they make zinc and insoluble compounds. Meanwhile, amino acids such as histidine, cystine, and tryptophan form a soluble complex with zinc, thereby improving the absorption of zinc.

Zinc is excreted through the pancreas through the intestines and into feces. It is also excreted in small amounts through urine and sweat.

The functionality of health functional foods ingested for the purpose of supplementing zinc, which is insufficient in daily meals, is a trace element present in all tissues of the body and is involved in the metabolism of nucleic acids and amino acids.

Blood and hair tests are used to measure the level of zinc in the body. The most widely used method is to measure zinc in blood, but in recent years, it is relatively easy and accurate to measure zinc in hair, so it is widely used as an indicator of zinc in the body.

Zinc is widely contained in foods, so extreme zinc deficiency is rare. Moderate zinc deficiency is commonly observed in diabetics. This is associated with increased excretion of zinc in the patient's urine.

Factors that cause zinc deficiency include insufficient intake of foods with low zinc content, decreased absorption of zinc due to decreased digestive function, and increased zinc needs due to surgery, burns, pregnancy, lactation, malignant tumors, etc. have.

When zinc is deficient, damage to the lining of the gastrointestinal tract or lung tissue is common, and it affects the function of multinuclear lymphocytes and natural killer cells. If zinc deficiency is severe, loss of appetite, growth retardation, skin changes, and immune function decline, etc., while severe zinc deficiency results in hypogonadism and dwarfism.

In particular, infants and young children may develop zinc deficiency because their zinc demand increases with rapid growth. Since a lack of zinc can cause chronic or acute diarrhea, a vicious cycle can be repeated as the body continues to lack zinc through diarrhea. Zinc deficiency in children can reduce immunity, cause frequent respiratory infections and wheezing when breathing.

Accordingly, in some countries, supplementation of zinc to children's diets has helped reduce the incidence of acute and chronic diarrhea and lower respiratory tract infections. Studies have shown that taking antibiotics and zinc together accelerates recovery from severe pneumonia.

Zinc is known to protect respiratory epithelial cells, suppress inflammatory reactions, and increase immunity. Therefore, when zinc is deficient, immunity against various pathogens such as viruses, bacteria, fungi, and parasites decreases, and thus, it is easily exposed to infectious diseases such as colds. Long-term use of more than 20 times the recommended amount of zinc (100-300 mg per day) leads to decreased copper and iron nutritional status, impaired immunity, and lowered HDL cholesterol. The maximum intake of zinc proposed by the World Health Organization (WHO) is 45 mg for adults and 35 mg for adult women.

Experimental example 1: Analysis of selenium content containing purslane

After harvesting purslane prepared according to the present invention, the selenium content was analyzed. About 500 g was collected, washed with distilled water, and dried to prepare a sample for analysis. 0.2 g of the obtained purslane sample was treated with mixed acid (nitric acid 5 ml + sulfuric acid 5 ml), heated (160℃/6hr), cooled, evaporated until gel, and filled with distilled water as much as the initial volume, and then inductively coupled plasma mass It was analyzed with a spectrophotometer (ICP-Mass spectrometer, ELAN6100, Perkin Elmer, USA; EPA Methods 2008, Rev 54).

The selenium content of the control group that was not fertilized with the active solution according to the present invention was 0.082-0.258 mg/kg, but the selenium content in the test group applied with the active solution according to the present invention was 0.467-0.816 mg/kg, according to the present invention. It was confirmed that the content of selenium was remarkably increased.

Experimental example 2: Analysis of zinc content containing purslane

After harvesting purslane prepared according to the present invention, the zinc content was analyzed. About 500 g was collected, washed with distilled water, and dried to prepare a sample for analysis.

In the zinc content test, the water-soluble zinc content was analyzed using the Dithizone method in accordance with the fertilizer quality inspection method and some amendments to the sampling standards (notified by the Rural Development Administration).

In the case of the present invention, the zinc content of the control group without application of the active solution according to the present invention was 1.012-1.052 mg/kg, but the zinc content in the test group fertilized with the active solution according to the present invention was 3.127-3.816 mg/kg. It was confirmed that the zinc content was remarkably increased.

In the above, the present invention has been described by way of example, but this is only exemplary, and those of ordinary skill in the art will understand that various modifications and equivalent embodiments are possible therefrom. Therefore, the true scope of protection of the present invention should be limited only by the appended claims.

Claims (2)

Preparing a mixed solution by mixing and heating selenium and zinc in a pulverized state in a brown rice vinegar solution;
Ionizing selenium and zinc by mixing and stirring alkaline water in the mixed solution;
Cooling and stabilizing the ionized mixture at room temperature;
Liquefying the active liquid by diluting the stabilized mixture with water; And
Purslane cultivation method comprising the step of fertilizing the liquefied active liquid with purslane.
The method of claim 1, wherein the step of fertilizing the liquefied active liquid to the crop is fertilized by repeatedly spraying and applying 3 to 5 times during the growth process of purslane.