KR20200099326A - Method for extracting isoflavones from soybean - Google Patents

Abstract

Introduced is a method for extracting isoflavones contained in soybean, in which dried and pulverized beans are extracted for 3 to 5 hours under reflux using 60 to 75% ethanol mixed with distilled water or distilled water as a solvent. It is possible to extract a large amount of aglycone components with high bioactivity, and the extracted isoflavone components can be used for food, beverage, etc.

Description

Method for extracting isoflavones from soybean}

The present invention relates to a method for extracting isoflavones contained in soybeans.

For modern people who are exposed to stimulating changing external environments, wrong lifestyles, and various stresses, skin aging prevention is a subject of constant and continuous interest, regardless of age or sex.

The appearance of skin aging is wrinkles. Aging that progresses through exposure to ultraviolet rays, which is an exogenous aging process, is mainly due to the expression of Matrix Metallo Proteases (MMPs), especially MMP-1. MMP-1 is an enzyme that decomposes collagen, and it is required to search for a physiologically active component to inhibit the expression of MMP-1 in order to improve skin wrinkles.

It is known that when the female hormone estrogen decreases, moisture and collagen in the skin decrease, leading to skin aging and wrinkles in the skin. Isoflavones have a molecular structure similar to estrogen and are known to have similar efficacy in our body. It is expected to prevent and improve skin aging, such as inhibition of MMP-1 expression using isoflavones that are naturally present in soybeans.

Soybeans are not only the largest source of protein in Korean diet, but are also used as a raw material for numerous chemical products. It consists of 8.6% moisture, 40% protein, 18% fat, 3.5% fiber, 4.6% ash, 4.4% pentosan, and 7% sugar.

Isoflavones are flavonoid-based substances derived from soybeans, and are composed of non-glycosides such as genistein, daidzein, glycitein, and their glycosides. Soybean isoflavone content is about 0.1 to 0.4%, and most of it is known to be in the form of glycosides such as genistin and daizin.

Research on isoflavones is being actively conducted both at home and abroad, and its main effects appear to be antioxidants, suppression of cancer induction, anti-inflammatory activity, and prevention of myocardial infarction and arteriosclerosis.

According to the article on the variation of isoflavone content in domestic soybean genetic resources [Journal of the Korean Society of Crop Science, 51(S):146~151(2006)], the average content of 43 major varieties of Korean soybeans was 484.4±223.9μg/ g, genistein is 448.4±156.2 μg/g, and glycitane is 84.9±48.7 μg/g.The average daidzein per 100 g of soybeans is 48.44 mg, genistein is 44.84 mg, and glycitane is 8.49 mg.

Because the content of isoflavones in the form of non-glycosylated soybeans is extremely low, isoflavones are extracted from soybeans using solvents that are not applicable for food, such as hydrochloric acid (1N-HCL), methanol, and hexane. Most of them were analyzed for ingredients. There is a need for an improved method for extracting isoflavones that can utilize isoflavones naturally present in soybeans for food or beverages.

The present invention is based on the recognition of the prior art as described above, to provide a more improved soybean-containing isoflavone extraction method so that the isoflavone component extracted from soy can be used as food.

The problem to be solved in the present invention is not necessarily limited to the above-mentioned matters, and other problems that are not already mentioned may be understood by the matters described below.

The soybean-containing isoflavone extraction method according to the present invention for achieving the above object is characterized in that after pulverizing the dried seonyu soybeans, the pulverized soybeans are subjected to reflux extraction using distilled water or ethanol mixed with distilled water as a solvent.

The ethanol mixed solvent is preferably a solvent of 60 to 75% ethanol in which a distillation value is mixed, and the extraction time is preferably 2 to 5, furthermore, 3 to 5 hours.

According to the soybean-containing isoflavone extraction method according to the present invention as described above, a relatively large amount of the isoflavone component can be extracted from the soybean, and since a chemical solvent such as hexane is not used, the extracted isoflavone component can be used as food. .

By reflux extraction, it is possible to extract a relatively large amount of non-glycoside components with high bioactivity than before.

1 shows an extraction method according to various examples of the present invention and a yield of isoflavones according thereto.
2 shows the yield of isoflavones by solvent conditions in the results of FIG. 1.
3 shows the yield of soflavones by extraction time from the results of FIG. 1.

Hereinafter, examples will be described in more detail in order to understand various characteristic aspects of the present invention. In the accompanying drawings, the same or equivalent components or parts may be indicated by the same reference numerals as possible for convenience of description, and the drawings are exaggerated and schematically illustrated for clear understanding and description of the features of the present invention. Can be.

In order to derive an optimal isoflavone extraction method that can be used for food or beverage, a study was conducted to extract isoflavones from soybeans using various methods. Finally, reflux extraction, cold needle extraction, and sonication extraction were selected as meaningful extraction methods, and among them, reflux extraction was concluded to be the most suitable.

Hereinafter, a method for extracting isoflavones containing soybeans will be described.

According to a joint research report on the development of new varieties of summer crops in 2004 and 2005, Seonyu beans have a higher composition of daidzein, genistein, glycitein and amino acids, which are non-glycosides of isoflavones, compared to the standard cultivar Taekwang. It is known as a variety that is fast and has a higher yield of 9% and can be grown anywhere in the country.

Seonyu soybeans with a high content of non-glycosylated isoflavones with anti-aging activity meet the search for the optimal extraction conditions for total isoflavone content, and furthermore, isoflavone content in food development and functional beverage development using extracts containing isoflavones It is expected to have competitiveness in In the experimental examples below, the soybeans were made from Jeonnam-san Seonyu.

For reflux extraction, the dried beans are pulverized with a grinder, and then 100 g of the crushed beans are put in 2L of distilled water (DW) equivalent to 20 times the amount of distilled water or alcohol mixed with distilled water, that is, ethanol (EtoOH). Scientific equipment, MS-E106, Korea) was used to repeat the process of extracting hot water. Distilled water was extracted by dividing into various conditions after reaching 100°C, and extraction with an ethanol mixed solvent was extracted for several hours after reaching 70-80°C. After extraction, the components were analyzed after filtration using a filter paper.

In the ultrasonic extraction, the dried beans are pulverized in the same manner as in the previous reflux extraction, and then hot water and solvent extraction are performed by dividing into various conditions in a constant temperature water bath at 40℃ using an ultrasonic cleaner (Sungdong Ultrasonic, SDd-350H, Korea). It proceeded in a way that was performed repeatedly. After extraction, the components were analyzed after filtration using a paper filter.

The ultrasonic extraction was carried out by pulverizing the dried beans with a grinder in the same manner as in the previous reflux extraction, and then repeatedly extracting under various conditions over 3 to 5 days in a 4°C refrigerated condition using an Erlenmeyer flask. After extraction, the components were analyzed after filtration using a paper filter.

Table 1 below shows several representative extraction conditions among various experimental examples, divided by extraction method, solvent, and time.

Table 2 below shows the yield of isoflavones according to the extraction conditions of Table 1. In order to adjust the deviation of the yield for each extraction condition, the average value was described after repeated extraction three times each.

Figure 1 shows the yield of isoflavones by extraction method. As shown in FIG. 1, the reflux extraction was found to have the highest yield, and the yield was significantly in the order of ultrasonic extraction and cold needle extraction, but it can be seen that there is no significant difference in yield under the two conditions of ultrasonic extraction and cold needle extraction.

Figure 2 shows the yield of isoflavones by method for each solvent. As shown in FIG. 2, 100% hot water extraction showed the highest yield, and when ethanol solvent was used, the yield was lower compared to hot water extraction, and in the two conditions of ethanol solvent 60% and 70%, the yield was large. There was no difference.

Figure 3 shows the isoflavone yield by extraction time. As shown in FIG. 3, there was no significant difference in yield between 3 hours and 5 hours in the reflux extraction method of the ethanol solvent, but the hot water extraction showed a relatively large difference regardless of the extraction method.

When the results are summarized in the above extraction method, solvent-specific method and extraction time, reflux extraction is the best extraction method. Although ultrasonic extraction showed a high yield of isoflavone extraction, large-scale mass production by ultrasonic extraction is likely to be difficult if the isoflavone extracted from soybeans is to be applied as food or beverage. On the other hand, 100% hot water extraction, which has a relatively strong polarity, shows a high yield, so it is judged that soybeans occupy a lot of components corresponding to relatively polarity.

Table 3 below shows the results of component analysis for six isoflavones extracted by the reflux extraction method.

As shown in Table 3, when looking at the results of the isoflavone content, samples with a relatively high total content of isoflavones in the form of glycosides were 10-A, 10-B, 10-C, 10-D, 10-J, 10-Q, 10- It can be seen that in the 60% EtOH reflux extraction method, the 60% EtOH cold needle extraction method, and the 100% distilled water ultrasonic extraction method with R, etc., relatively large amounts are detected regardless of the extraction time. In particular, the 60% EtOH cold needle extraction method for samples 10-C and 10-D is judged to be the most optimized extraction method for extracting isoflavones in the form of glycosides.

It can be seen that the samples with a relatively high total content of isoflavones in the form of non-glycosides, which are active ingredients, are 10-E, 10-F, 10-G, and 10-H, which appear to show a relatively high content. . The 70% EtOH reflux extraction method corresponding to 10-G and 10-H has a relatively high total content of isoflavones in the form of glycosides as well as the total content of isoflavones in the form of a glycoside as an active ingredient. Of these, the 70% EtOH reflux 5hr extraction method, sample 10-H, showed a relatively high content of isoflavones in the form of glycosides as well as in the non-glycoside form, and is the most optimized for extracting isoflavones naturally present in soybeans. It is considered an extraction method.

Based on the 70% EtOH reflux 5-hour extraction method established as the optimum extraction condition for isoflavones, the extraction time was increased to add 7 hours to establish a clearer extraction condition, and the amount of solvent was increased by 30 times and 40 times the amount of the raw material. Research was conducted by adding conditions, and the results are shown in Tables 4 and 5 below.

As shown in Table 4 and Figure 5, the content of isoflavones in the form of glycosides was relatively higher as the extraction time and the amount of solvent increased, and the isoflavones in the form of non-glycosides were extracted at 7 hours than the established optimal extraction conditions. It can be seen that a relatively higher content was shown, but the difference in content change did not appear significantly with the amount of the solvent.

Overall, the content of isoflavones may appear relatively high when the extraction time and the amount of solvent are increased compared to the 70% EtOH reflux 5 hours established as the optimum extraction condition for isoflavones, but 70% compared to the time and cost invested in commercialization. EtOH reflux conditions for 5 hours can be determined to be more suitable extraction conditions for productivity.

Although shown and described above with respect to specific embodiments of the present invention, it is necessary to understand that the present invention can be variously modified or modified within the scope not departing from the technical spirit of the invention described in the following claims.

Claims (1)

Soybean-containing isoflavone extraction method, characterized in that after pulverizing the dried Seonyu beans, using a reflux device to extract the pulverized beans using distilled water or 60-75% ethanol mixed with distilled water as a solvent for 3 to 5 hours .

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