KR20200093342A - A device for producing a suspension for fecal microbiota transplantation - Google Patents

Abstract

The present invention relates to a suspension manufacturing apparatus capable of reducing discomfort and sense of repulsion of medical staff, donors, and patients without a manufacturing space such as a separate air purification facility, and collecting, dissolving, and filtering in order to manufacture a fecal suspension for fecal microbiota transplantation while minimizing contact with air during manufacture.

Description

Fecal fluid manufacturing apparatus for fecal microbial transplantation {A DEVICE FOR PRODUCING A SUSPENSION FOR FECAL MICROBIOTA TRANSPLANTATION}

The following descriptions relate to a fecal fluid manufacturing apparatus for fecal microbial transplantation, specifically reducing the discomfort and rejection of medical staff, donors, and patients without a manufacturing space such as a separate air purification facility, and minimizing contact with air during manufacturing. The present invention relates to a suspension manufacturing apparatus capable of collecting, dissolving, and filtering to prepare a fecal suspension for fecal microbial transplantation.

Fecal microbial transplants include Clostridium difficile infection, pseudomembranous enteritis, Crohn's disease, ulcerative colitis, traveler's diarrhea, hypersensitivity syndrome, small intestinal bacterial hyperplasia, non-alcoholic fatty liver, hepatic encephalopathy, hepatobiliary autoimmune disease, joint disease , Influenza virus infection, dementia, depression, multiple sclerosis, obesity, diabetes can be applied to the treatment of various diseases. In order to prepare a suspension for fecal microbial transplantation, in the past, a feces received from a donor at a place equipped with an air purification facility such as a fume hood was prepared and used with a blender. At this time, there was discomfort and rejection in the process of preparing the suspension, and the suspension could not be prepared in a place where the fume discharge hood was not equipped. In addition, due to the high contact with air in the manufacturing process, it could have a great influence on the anaerobic microorganisms in the feces. Therefore, there is a need for a device capable of producing fecal suspension by minimizing contact with air and manufacturing without discomfort and reluctance even in a place without an air purification facility such as a fume exhaust hood.

The present invention relates to a suspension manufacturing apparatus for producing a fecal suspension by performing fecal collection, dissolution, and filtration from a donor while minimizing contact with air in a space-limited place without a separate air purification facility.

The present invention relates to a suspension manufacturing apparatus capable of producing a suspension for fecal microbial transplantation, without the inconvenience and rejection of producing a fecal suspension.

According to an embodiment, the rotating unit is rotated through a rotating handle at an upper end of an axis to which the rotating unit is attached, and the lower end of the shaft is a mixing means coupled to one surface of the fecal collecting means; Fecal collection means for collecting feces from a donor and temporarily holding feces mixed and dissolved by the mixing means in combination with one side of the mixing means; And filtration and storage means combined with one side of the feces collection means and storing the filtered fecal suspension through a filtration net and filter paper, wherein the mixing means, the feces collection means and the filtration and storage means are one by detachment. It can be a suspension preparation device for fecal microbial transplantation, which can be combined.

The rotating unit and the rotating handle of the mixing means may include a plurality, and may be a fecal suspension manufacturing apparatus for fecal microbial transplantation, in which the feces are mixed and dissolved by rotation of each rotating unit corresponding to each rotating handle. have.

Suspension for fecal microbial transplantation, in which a plurality of rotating units attached to the shaft are attached along the axis, and the plurality of rotating units are rotated and dissolved by mixing the feces by the rotating handle on the top of the shaft. It may be a manufacturing device.

The rotating unit attached to the shaft may be a suspension manufacturing apparatus for fecal microbial transplantation, which is composed of a plurality of wires and melts while the feces are mixed by rotation of the wires.

The plurality of wires may be a suspension manufacturing apparatus for fecal microbial transplantation, in which the heights on the axis are disposed at the same place or at different heights on the axis.

The rotating unit may be a fecal fluid manufacturing apparatus for fecal microbial transplantation, having a structure in which a plurality of rotating blades positioned at the lower end of the shaft are bent at a constant angle so as to be symmetrical about the axis.

The filtration and storage means may be a suspension manufacturing apparatus for fecal microbial transplantation, filtering suspended matter from the dissolved feces using a filter screen or filter paper.

The manure paper may be a suspension manufacturing apparatus for fecal microbial transplantation, having a membrane size such that a microorganism contained in the mixed feces penetrates.

According to an embodiment, the mixing means for mixing the feces through a rotating unit whose rotational speed is controlled by a motor; Fecal collection means for collecting feces from a donor and temporarily holding feces mixed and dissolved by the mixing means in combination with one side of the mixing means; And filtration and storage means combined with one side of the feces collection means and storing the filtered fecal suspension through a filtration net and filter paper, wherein the mixing means, the feces collection means and the filtration and storage means are one by detachment. It can be a suspension preparation device for fecal microbial transplantation, which can be combined.

The rotational speed is a preset speed, and may be a suspension manufacturing apparatus for fecal microbial transplantation, which is changed based on the degree of mixing of the feces.

The rotating unit may be a suspension manufacturing apparatus for fecal microbial transplantation in which a plurality is attached along an axis and the feces are mixed by rotation of the plurality of rotating units by the motor.

The rotating unit may be a suspension manufacturing apparatus for fecal microbial transplantation, which is composed of a plurality of wires and the feces are mixed by rotation of the wires.

The plurality of wires may be a suspension manufacturing apparatus for fecal microbial transplantation, in which the heights on the axis are disposed at the same place or at different heights on the axis.

The rotating unit may be a fecal fluid manufacturing apparatus for fecal microbial transplantation, having a structure in which a plurality of rotating blades located at the lower end of the shaft are bent at a constant angle so as to be symmetrical about the axis.

The filtration and storage means may be a suspension manufacturing apparatus for fecal microbial transplantation, filtering suspended matter from the dissolved feces using a filter screen or filter paper.

The manure paper may be a suspension manufacturing apparatus for fecal microbial transplantation, having a membrane size such that a microorganism contained in the mixed feces penetrates.

According to one embodiment, the fecal suspension manufacturing apparatus is capable of producing fecal suspension by minimizing contact with air by performing fecal collection, dissolution, and filtration from a donor in a space-limited place without a separate air purification facility. have.

According to an embodiment, the apparatus for producing a fecal suspension can prepare a suspension for transplanting fecal microorganisms without the inconvenience and reluctance of preparing the suspension.

1 shows an apparatus for producing feces for transplanting feces microorganisms, according to an embodiment.
Figure 2 shows a fecal fluid manufacturing apparatus for fecal microbial transplant, according to another embodiment.
3 shows a mixing means in a fecal fluid production apparatus for fecal microbial transplantation, according to an embodiment.
Figure 4 shows a rotating unit of the mixing means in the fecal fluid production apparatus for fecal microbial transplantation, according to one embodiment.
Figure 5 shows a rotating unit of the mixing means in the fecal fluid manufacturing apparatus for fecal microbial transplant, according to another embodiment.
Figure 6 shows a rotating unit of the mixing means in the fecal fluid production apparatus for fecal microbial transplant, according to another embodiment.
Figure 7 shows a rotating unit of the mixing means in the fecal fluid production apparatus for fecal microbial transplant, according to another embodiment.
Figure 8 shows a feces collection means for collecting feces from a donor and combining it with one side of the mixing means in a feces preparation device for fecal microbial transplantation, according to one embodiment.
9 and 10 show a filtration and storage means in a fecal fluid manufacturing apparatus for fecal microbial transplantation, according to an embodiment.

Hereinafter, embodiments will be described in detail with reference to the accompanying drawings. However, the scope of the patent application is not limited or limited by these embodiments. The same reference numerals in each drawing denote the same members.

Various modifications may be made to the embodiments described below. The examples described below are not intended to be limiting with respect to the embodiments, and should be understood to include all modifications, equivalents, or substitutes thereof.

Terms such as first or second may be used to describe various components, but these terms should be understood only for the purpose of distinguishing one component from other components. For example, the first component may be referred to as the second component, and similarly, the second component may also be referred to as the first component.

The terms used in the examples are only used to describe specific examples, and are not intended to limit the examples. Singular expressions include plural expressions, unless the context clearly indicates otherwise. In this specification, terms such as “include” or “have” are intended to indicate that there are features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, and one or more other features. It should be understood that the existence or addition possibilities of fields or numbers, steps, operations, components, parts or combinations thereof are not excluded in advance.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person skilled in the art to which the embodiment belongs. Terms, such as those defined in a commonly used dictionary, should be interpreted as having meanings consistent with meanings in the context of related technologies, and should not be interpreted as ideal or excessively formal meanings unless explicitly defined in the present application. Does not.

In addition, in the description with reference to the accompanying drawings, the same reference numerals are assigned to the same components regardless of reference numerals, and redundant descriptions thereof will be omitted. In describing the embodiments, when it is determined that detailed descriptions of related known technologies may unnecessarily obscure the subject matter of the embodiments, the detailed descriptions will be omitted.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 shows an apparatus for producing feces for transplanting feces microorganisms, according to an embodiment.

According to one embodiment, the present invention relates to an apparatus for preparing a suspension for treatment in a feces of a donor for Fecal Microbiota Transplantation (FMT), which is emerging as a new treatment method for various diseases. At this time, fecal microbial transplantation refers to a technique of delivering and treating a gut microorganism of a healthy donor to a patient whose disease or gut microbial pathology has changed. Here, the feces described in the present invention is not limited to being replaced by expressions of suspensions, liquids and the like as an example.

The present invention relates to a manufacturing apparatus for producing a suspension by collecting, dissolving and mixing through the mixing of feces received from a donor for such fecal microbial transplantation. In other words, in order to implant feces from a donor, it is necessary to prepare a suspension in a liquid state so that it is easy to spray on the patient's intestine. 1 shows an apparatus for preparing a suspension from feces of donors.

The suspension production apparatus shown in FIG. 1 may include mixing means 110, feces collection means 120, filtration and storage means 130. Here, the mixing means 110 and the feces collection means 120, the filtration and storage means 130 can be detachable, respectively, can be combined into one to prepare a fecal suspension. Here, the apparatus for manufacturing feces may be configured by detaching the mixing means 110 and the feces collection means 120, the filtration and storage means 130, or the mixing means 110 and the feces collection means 120, Filtration and storage means 130 may be integrally configured. Alternatively, the apparatus for manufacturing a fecal fluid is a mixing means 110, a feces collection means 120, a filtration and storage means 130, a mixing means 110 and a filtration and storage means 130 are integral and intermediate fecal collection means Only 120 can be detached, and the detached feces collection means 120 can be used to collect feces from the donor.

The mixing means 110 may dissolve feces received from the donor by mixing. At this time, the mixing means 110 may include a rotating unit, and the feces may be mixed and dissolved by rotation of the rotating unit by the rotating handles 101 and 102. At this time, sterilized physiological saline or distilled water is introduced through the inlet 112 to be used to dissolve solid feces. See FIG. 3 for a specific process for dissolution of feces by mixing.

The feces collection means 120 may collect feces from donors. Specifically, the feces collection means 120 is detachable, and can be used to collect feces from donors. The mixing means 110 and the feces collection means 120 may be combined through a detachable stopper 111, and the feces contained in the feces collection means 120 may be dissolved while being mixed by the mixing means 110. At this time, when the dissolved feces move to the filtration and storage means 130 through the lower end of the feces collection means 120, it may be filtered in the process of moving undissolved debris. 8 for a detailed description of the feces collection means.

The filtration and storage means 130 may be combined with the feces collection means 120 through a detachable stopper 121. The dissolved, filtered suspension can be filtered from the fecal collection means 120 through a filtration net and filter paper to move to the filtration and storage means 130. The suspension introduced into the filtration and storage means 130 can be directly transplanted into the patient by suction with a syringe or the filtration and storage means detached from the feces collection means itself can be stored in a frozen state. 9 and 10 for a detailed description of filtration and storage means.

According to one embodiment, the treatment of Clostridium difficile infection, pseudomembrane enteritis through transplantation of fecal suspension, as well as Crohn's disease, ulcerative colitis, traveler's diarrhea, irritable growth syndrome, hyperplasia of small intestine bacteria , Non-alcoholic fatty liver, hepatic encephalopathy, hepatobiliary autoimmune disease, joint disease, influenza virus infection, dementia, depression, multiple sclerosis, obesity, diabetes, etc. are also effective. Such diseases are only an example through fecal microbial transplantation, and other diseases are also effective for fecal microbial transplantation.

Figure 2 shows a fecal fluid manufacturing apparatus for fecal microbial transplant, according to another embodiment.

According to an embodiment, unlike the rotation handles 101 and 102 shown in FIG. 1, the rotation unit may be rotated by the motor 240. At this time, the rotation speed of the rotating unit by the motor may be changed based on the degree of mixing of the feces. Specifically, when the feces are close to a solid or when the feces are mixed and close to a suspension, the feces can be dissolved while mixing at different rotational speeds.

According to one embodiment, the rotational speed of the motor may be set in advance. Specifically, the rotation speeds of different motors are applied according to a preset time, so that the feces can be mixed by the rotation unit. For example, when the power of the motor is set to on, the rotational speed of the motor applied from 1 minute to after the motor is operated, the rotational speed of the motor applied from 1 minute to 5 minutes, and the motor applied from 5 minutes to 6 minutes. The speed of rotation can be different. At this time, the rotation speed and time of the motor may be set in advance by the user.

3 shows a mixing means in a fecal fluid production apparatus for fecal microbial transplantation, according to an embodiment.

The mixing means can be combined with the fecal collection means through a detachable stopper (307). The mixing means includes a shaft to which the rotating units 311 and 312 are attached, and the upper end of the shaft is connected to the rotating handles 301 and 302 for rotating the rotating units 311 and 312, and the lower end of the shaft is of the fecal collecting means. It can be combined with one side. The shape of the rotation units 311 and 312 shown in FIG. 2 is only an example, and the scope of the present invention is not limited to the shape of the rotation units 311 and 312 shown in FIG. 2, and the feces are rotated through the rotating unit. Other shapes for mixing may also be included in the scope of the present invention.

According to one embodiment, when the rotating handle 301 is rotated, the rotating unit 311 corresponding to the rotating handle 301 may rotate while mixing the donor's feces, or rotate when the rotating handle 302 is rotated The rotating unit 312 corresponding to the handle 302 rotates to mix donor feces. Alternatively, when one rotary handle is rotated, the donor feces can be mixed while the rotation units 311 and 312 are all rotated.

At this time, sterilized physiological saline or distilled water may be input to the input port 304 through the input port stopper 303. The mixing means may dissolve feces while mixing feces with sterilized physiological saline or distilled water. In this process, a process in which feces are dissolved through the transparent plate 306 may be observed. That is, while checking the degree of dissolution of feces through the transparent plate 306, the rotational speed of the rotating unit can be adjusted.

Figure 4 shows a rotating unit of the mixing means in the fecal fluid production apparatus for fecal microbial transplantation, according to one embodiment.

According to one embodiment, the rotating unit may mix and dissolve feces collected from the donor. At this time, the rotating unit may have various shapes, and the shape of the rotating unit shown in FIG. 4 is only an example, and the scope of the present invention is not limited thereto.

A plurality of rotating units may be attached along an axis, and feces may be dissolved while mixing by rotating the rotating unit. For example, the rotating unit attached to the shaft can be rotated, and in order to widen the area in contact with the feces to more easily dissolve the feces, the rotating unit may have a shape as shown in FIG. 4.

The rotation unit 410 and the other rotation unit 420 shown in FIG. 4 may be disposed at the same height and spaced apart from the center of the axis. The rotation unit 410 and the rotation unit 420 shown in FIG. 4 represent a state disposed in an opposite direction of 180 degrees around the axis, and the present invention is not only disposed in an opposite direction of 180 degrees but also arranged by another angle It can also be included in the scope of rights.

The rotation unit 410 and the rotation unit 430 shown in FIG. 4 may be disposed at different heights around the axis. At this time, different heights to be arranged may be set in consideration of feces.

Figure 5 shows a rotating unit of the mixing means in the fecal fluid manufacturing apparatus for fecal microbial transplant, according to another embodiment.

5 shows a shape of a rotation unit different from FIG. 4. At this time, the rotating unit may be composed of wires, and the start and end points of the plurality of wires may be arranged at the same position on the axis. Thus, the feces can be dissolved while mixing by the rotation of the rotating unit composed of wires.

Figure 6 shows a rotating unit of the mixing means in the fecal fluid production apparatus for fecal microbial transplant, according to another embodiment.

FIG. 6 shows the shape of the rotation unit different from FIG. 5. As in Fig. 5, the rotating unit may be composed of wire. However, unlike FIG. 4, the start and end points of the wire may be arranged at different positions of the axis. Thus, each wire can rotate different regions, and feces can be dissolved while mixing.

7 shows a rotating unit of a mixing means in a fecal fluid production apparatus for fecal microbial transplantation, according to another embodiment. In the case of a mixing means rotating by a motor, a plurality of rotating units having a shape of a rotating blade as shown in FIG. 7 may be included.

7, the rotation units 710 to 730 have a rotation blade shape that is symmetrical about an axis, and the rotation unit 720 disposed on the rotation unit 710 rotates a different area from the rotation unit 710 Can. In addition, the rotation unit 730 represents a state arranged at 90-degree intervals with the rotation units 710 and 720, and the present invention can include a state arranged at a different angle as well as a state arranged at 90 degrees. .

A and B of the rotation units 710 to 730 may have a structure bent at a constant angle as shown in FIG. 7, and at this time, the angles of the rotation units 710 to 730 may be different from each other.

Figure 8 shows a feces collection means for collecting feces from a donor and combining it with one side of the mixing means in a feces preparation device for fecal microbial transplantation, according to one embodiment.

Fecal collection means can be detached and used to collect feces from donors. At this time, the sealing stoppers 810 and 811 may serve to seal the feces collected inside the manure means. The sealing stopper 810 may block the smell of feces that may give a feeling of rejection, and the sealing stopper 811 may prevent leakage of feces due to a malfunction of the handle 820. The sealing stoppers 810 and 811 combined with the feces collecting means may be in the form of rubber, or may have a form of sealing by turning.

After removing the sealing stopper 810, the corresponding portion may be combined with one surface of the mixing means, and after removing the sealing stopper 811, the corresponding portion may be combined with one surface of the filtering and storage means.

The fecal collection means is attached to mixing means, filtration and storage means, and can be used to make a fecal suspension. At this time, the feces collected in the feces collection means may be dissolved while being mixed by the mixing means, and the dissolved feces may be filtered while moving through a filtering screen and a filtering device to produce a fecal suspension.

At this time, the handle 820 may serve to open and close the passage between the feces collection means and the filtration and storage means. That is, when the handle is closed (821), the dissolved fecal liquid in the feces collection means cannot be moved to the storage means, and when the handle is opened (822), the dissolved feces in the feces collection means can be moved to the storage means.

At this time, the handle 820 shown in FIG. 8 is for expressing that the opening and closing role between the manure means and the storage means. Accordingly, the scope of the present invention is not limited to the shape of the handle shown in FIG. 8, and other forms capable of opening and closing the passage between the manure means and the storage means may also be included in the scope of the present invention.

9 and 10 show filtering and storage means in a suspension manufacturing apparatus for fecal microbial transplantation, according to an embodiment.

The filtration and storage means may include a suspension that is filtered dissolved feces when the handle shown in FIG. 8 is opened. At this time, the filter screens 950, 1050 (for example, wire, gold, etc.) can be used to filter out large sized debris, and through the filter paper 960, 1060, small sized debris can be filtered out.

At this time, the filter network 950 and the filter paper 960 may have a flat shape as shown in FIG. 9, or the filter network 1050 and the filter paper 1060 may have a concave downward shape as shown in FIG. Can have Specifically, the center portion of the filter network 1050 and the filter paper 1060 may have a concave shape downward. As such, in the case of a concave downward direction, it is possible to better filter the suspended solids contained in the fecal fluid moving according to gravity.

The dissolved feces can be filtered, moving from the feces collection means to the filtration and storage means. For filtration, filtration nets 950 and 1050 and manure papers 960 and 1060 may be used, and the manure papers 960 and 1060 may have a membrane size such that microorganisms contained in dissolved feces penetrate. Thus, the microorganisms contained in the dissolved feces can move through the manure paper (960, 1060), and the residue can be filtered out of the manure paper (960, 1060).

Suspensions containing fecal microorganisms in the storage means may be divided into certain doses. Specifically, the syringes 970 and 1070 may be connected to the solution suction portions 940 and 1040 of the storage means using hoses 980 and 1080 and detachable stoppers 930 and 1030. Therefore, the suspension can be divided into a certain volume through a syringe.

If the suspension, divided by a given dose, is not immediately transplanted to the patient, the suspension can be stored at a temperature capable of storing bacteria without deterioration and can be transplanted to the patient whenever necessary. At this time, the suspension may be sprayed into the patient's large intestine or duodenum through a colonoscopy or gastroscopy, and fecal microorganisms from healthy donors may be implanted into the patient's intestines.

Claims (16)

Mixing means for rotating the rotating unit through a rotating handle on the top of the shaft to which the rotating unit is attached, the lower end of the shaft is coupled to one surface of the fecal collection means;
Feces collection means including feces that are dissolved while the feces collected from the donor are mixed by the rotating unit of the mixing means; And
Filtration and storage means combined with one side of the feces collection means and storing the suspension filtered by the filter net and filter paper
Including,
The mixing means, the feces collection means and the filtration and storage means can be combined by desorption, feces liquid manufacturing apparatus for fecal microbial transplant.
According to claim 1,
A rotating unit and a rotating handle of the mixing means include a plurality of, and the fecal solution manufacturing apparatus for fecal microbial transplantation, wherein the feces are mixed and dissolved by rotation of each rotating unit corresponding to each rotating handle.
According to claim 1,
A plurality of rotating units attached to the shaft is attached along the shaft,
Fecal fluid manufacturing apparatus for fecal microbial transplantation, wherein the plurality of rotating units are rotated by the rotating handle on the upper end of the shaft to dissolve while the feces are mixed and dissolved.
According to claim 1,
The rotating unit attached to the shaft is composed of a plurality of wires,
Fecal fluid manufacturing apparatus for fecal microbial transplantation, wherein the feces are mixed and dissolved by rotation of the wire.
According to claim 4,
The plurality of wires,
The height on the axis is the same, or
Fecal fluid manufacturing apparatus for the fecal microbial transplant, which is disposed at different heights on the axis.
According to claim 1,
The rotating unit,
Fecal fluid manufacturing apparatus for fecal microbial transplantation, having a structure in which a plurality of rotating blades located at the lower end of the shaft is bent at a constant angle so as to be symmetric about the axis.
According to claim 1,
The filtration and storage means,
A fecal fluid manufacturing apparatus for fecal microbial transplantation that filters the dissolved feces using a filter net or filter paper and stores the dissolved feces.
The method of claim 7,
The filter paper,
A device for producing feces for transplanting feces microorganisms having a membrane size such that the microorganisms contained in the mixed feces are permeable.
Mixing means for mixing the feces through a rotating unit whose rotational speed is controlled by a motor;
Fecal collection means comprising feces collected from a donor and combined with one side of said mixing means to be mixed and dissolved by said mixing means; And
Filtration and storage means combined with one side of the feces collection means and storing the suspension filtered by the filter net and filter paper
Including,
The mixing means, the feces collection means and the storage means can be combined by detachment, feces liquid manufacturing apparatus for fecal microbial transplant.
The method of claim 9,
The rotational speed is a preset speed, and is changed based on the degree of mixing of the feces, a fecal fluid manufacturing apparatus for fecal microbial transplantation.
The method of claim 9,
A plurality of the rotation unit is attached along the axis,
A fecal fluid manufacturing apparatus for fecal microbial transplantation in which the feces are mixed by the rotation of the plurality of rotating units by the motor.
The method of claim 9,
The rotating unit is composed of a plurality of wires,
The feces are mixed by the rotation of the wire, feces liquid manufacturing apparatus for fecal microbial transplant.
The method of claim 12,
The plurality of wires,
The height on the axis is the same, or
Fecal fluid manufacturing apparatus for the fecal microbial transplant, which is disposed at different heights on the axis.
The method of claim 9,
The rotating unit,
Fecal fluid manufacturing apparatus for fecal microbial transplantation, having a structure in which a plurality of rotating blades located at the lower end of the shaft is bent at a constant angle so as to be symmetric about the axis.
The method of claim 9,
The filtration and storage means,
A fecal fluid manufacturing apparatus for fecal microbial transplantation that filters the dissolved feces and stores the feces using a filter net or filter paper.
The method of claim 15,
The filter paper,
A device for producing feces for transplanting feces microorganisms having a membrane size such that the microorganisms contained in the mixed feces are permeable.

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