KR20200086091A - Pipette tip for preventing loss of sample - Google Patents

Abstract

The present invention relates to a pipette tip for preventing loss of a sample. Particularly, the pipette tip includes: a top portion configured to be attached to a pipette and having a hollow cylindrical inner part; a medium portion connected to the top portion and having a hollow inner part in the form of a combination of two truncated cones; and a bottom portion connected to the medium portion, having a hollow cylindrical inner part, and provided with 1-50 pores having a diameter of 0.1 mm and perforated on the section of the bottom end. Since the pipette tip for preventing loss of a sample has pores having a diameter of 0.1 mm and perforated on the bottom end, it can prevent loss of a sample, such as spheroids or organoids, during the exchange of a culture medium, cleaning of cells or transfer of tissues, and thus can improve inaccurate test results caused by loss of a sample according to the related art. In addition, the pipette tip according to the present invention is amenable to mass production with low costs, and thus shows high cost-efficiency.

Description

Pipette tip for preventing loss of sample}

The present invention relates to a pipette tip for preventing the loss of a sample, specifically, a sample that can prevent the sample from being lost in an experiment using a sample such as a small tissue, a spheroid, or an organoid. It relates to a pipette tip for prevention.

Micro-pipettes are basic devices used to measure or transfer small amounts of samples in the chemical, life science and medical fields. It is widely used in all areas where relatively small amounts of liquid (eg in the microliter range) must be metered, for example in molecular biology. Once used, the pipette is left in a very small amount due to the sample inside, so it is prohibited to reuse it to secure the stability of the precision analysis test. Therefore, as a countermeasure for this, a disposable pipette tip is inserted into the tip of the pipette.

In general, pipette tips are classified into three products according to their capacity, and various products with special functions are also sold and used. Currently, three products, standard micro pipettes of 10 µl, 200 µl, and 1000 µl, account for approximately 60% of the total market, and functional products account for approximately 40%.

On the other hand, in recent years, the culture of spheroids, which are three-dimensional cell tissues having the same function as in vivo, has attracted attention, and induces cell aggregation to mimic cancer, and the use of insulin for the treatment of diabetes. In order to induce normal secretion, a method of transplanting aggregated cells is used in transplanting islet cells, and mass production of spheroids is required. In addition, as the stem cell research matures, various methods have been attempted to culture embryonic stem cells in three dimensions and apply them to studies of various differentiation mechanisms.

Thus, these three-dimensional cell culture methods include the effects of regenerative medicine, hybrid artificial organs, bio-useful substance production, investigation and exploration of functions of biological tissues or organs, screening of new drugs, and endocrine disrupting substances. It is expected to be used in industries in each field, such as alternative methods for evaluating animal experiments and cell chips with sensor function.

However, in such a three-dimensional cell culture or experiment, when a commercially available pipette tip is used when replacing the medium, spheroid or organoid cells are attached to the pipette tip and the cell sample is frequently lost. There is a problem and it is necessary to develop a new pipette tip to prevent this.

Korean Registered Patent No. 10-1785441

Accordingly, the present inventors have completed the present invention by developing a new type of pipette tip that can prevent the loss of samples such as spheroid or organoid cells and is economically inexpensive.

Accordingly, an object of the present invention is to provide a pipette tip for preventing sample loss, which can prevent the loss of a sample, such as a spheroid or organoid cell.

In order to achieve the above object, the present invention is an upper portion with an internal hollow cylindrical portion attached to the pipette; A middle part connected to the upper part and having an empty inside in a form of a combination of two cut cones; And it is connected to the middle portion and the inside is cylindrically hollow, and the end section is provided with a pipette tip for preventing sample loss, including 1 to 50 holes having a diameter of 0.1 mm.

In one embodiment of the invention, the sample may be a tissue, a spheroid, or an organoid.

In one embodiment of the present invention, the upper end is detachable with the pipette, the outer diameter is 5mm to 7mm, the first inner diameter may be 3.5mm to 4.5mm.

In one embodiment of the present invention, the second inner diameter of the middle portion may be 2.5 mm to 3.4 mm, and the third inner diameter may be 0.5 mm to 2.4 mm.

In one embodiment of the present invention, the pipette tip may have a total length of 10 mm to 30 mm.

In one embodiment of the present invention, the pipette tip may be made of plastic.

In one embodiment of the invention, the plastic is polyethylene (polyethylene, PE), polypropylene (polypropylene, PP), polystyrene (polystyrene, PS), polyethylene terephthalate (polyethylene terephthalate, PET), polyamides (polyamides, PA ), polyester (PES), polyvinyl chloride (PVC), polyurethane (polyurethanes, PU), polycarbonate (PC), polyvinylidene chloride (PVDC), polytetrafluor It may be selected from the group consisting of ethyl (polytetrafluoroethylene, PTFE), polyetheretherketone (PEEK) and polyetherimide (PEI).

The pipette tip for preventing the loss of a sample according to the present invention has a hole of 0.1 mm at its lower end, in an experiment using a spheroid or organoid sample, a tip when replacing the medium ) Has the effect of preventing the sample from being lost due to the accompanying spheroid or organoid, thereby preventing inaccurate experimental results due to the loss of the conventional sample, and also has an economical effect that can be produced in large quantities at a low cost. .

1 is a view showing the structure and size of the pipette tip according to the present invention.
FIG. 2 is a view showing a pipette tip according to the present invention as viewed vertically from the bottom.
3 is a view showing a cross section when the pipette tip according to the present invention is viewed vertically from the top.
4 is a view showing a perspective view of a pipette tip according to the present invention.
5 is a view showing the contents of the use of the pipette tip according to the present invention.

The present invention relates to a pipette tip for preventing sample loss, and the pipette tip for preventing sample loss according to the present invention includes a top portion having a cylindrical interior and an empty portion attached to the pipette; A middle part connected to the upper part and having an empty inside in a form of a combination of two cut cones; And a lower end which is connected to the middle end and is hollow in a cylindrical shape, and has 1 to 50 pores having a diameter of 0.1 mm on the end section.

In particular, the pipette tip for preventing sample loss according to the present invention can prevent the loss of the samples in an experiment process dealing with samples such as tissue or spheroids or organoids larger in size than ordinary cells. It was made to be.

In the above, "tissue" is one of the units constituting an organism, and refers to a cell mass having a three-dimensional shape having the same shape or function.

In addition, the “spheroid” has a small “spheroid” shape created through three-dimensional cell culture, and the spheroid is a small “tissue” that is divided into the outside and the inside, and functions are divided. It takes the form of simulating the functional unit of. Spheroid culture is not only similar in nature and nature to animals or human tissues, but is also being developed as a platform that can be applied to research by applying it. For example, spheroids made using cancer cells have a more similar form to tumor tissues in vivo, so the efficacy of anticancer agents is more accurate than that of flat cultured cancer cells. It has been reported that when hepatocellular carcinoma cells are cultured as spheroids, hepatotoxicity test results closer to in vivo data can be obtained than results from the second plane.

The term “organoid” used in the present invention refers to a cell mass having a 3D conformation, and refers to a reduced and simplified version of an organ manufactured through an artificial culture process that is not collected and obtained from animals or the like. . The origin of the cells constituting it is not limited. Organoids can be derived from tissue, embryonic stem cells or induced pluripotent stem cells, and can be cultured in three dimensions due to their ability to regenerate and differentiate themselves. The organoid may have an environment that is allowed to interact with the surrounding environment during the cell growth process. Therefore, 3D organoids can be used as a good model for the development of therapeutic agents for diseases because they can almost completely simulate organs that actually interact in vivo.

The term “cell” used in the present invention is a functional and structural basic unit of all living organisms, mammary epithelial cells, epithelial cells, skin cells, endothelial cells, vascular cells, bone cells, osteoblasts, chondrocytes, fibroblasts. , Muscle cells, adipocytes, nerve cells, immune cells, blood cells, connective tissue cells, and various adult cells, as well as stem cells and hematopoietic cells, cells that can differentiate into a variety of cells are meant to include all of the It is not limited to kind.

The term “three-dimensional cell culture” used in the present invention is a three-dimensional cell culture method capable of cultivating tissues similar to real tissues by simulating the environment in vivo in consideration of the spatial organization of cells. Various types of supports such as hydrogels, capsules, and sponges are used for dimensional cell culture. Such a support requires biocompatibility, biodegradability, mechanical strength, and cell activity control ability to provide a basis for cells to attach and proliferate and differentiate.

The term “pipette tip” used in the present invention refers to a disposable container that is used to move a certain amount of liquid to be contaminated without being contaminated. The specific structure and size of the preventive pipette tip is shown in FIG. 1.

If the structure of the pipette tip for preventing sample loss of the present invention is described in more detail, it is largely composed of an upper portion, a middle portion, and a lower portion, and the upper portion can be attached to the pipette and the inside is cylindrically hollow. In addition, the upper end can be attached and detached with a pipette, the outer diameter is 5mm to 7mm, preferably 6mm. In addition, the first inner diameter of the upper end may be 3.5mm to 4.5mm, preferably 4mm.

The middle part is connected to the upper part and the inside is empty in the form of two cut cones combined. In the form of the middle end, one conical shape is connected to the upper end, and the other conical shape is connected to the lower end.

Since the middle part has the same structure as the shape of two cones combined, the inside diameters of different sizes are used, and the second inner diameter of the middle part is the diameter of the boundary portion where the two cut cone shapes cut together are 2.5 mm to 3.4mm, and preferably 3mm, but is not limited to this if it is within a range smaller than the first inner diameter.

In addition, the third inner diameter of the middle portion may be 0.5 mm to 2.4 mm, preferably 1 mm, as the inner diameter of the boundary portion from the middle portion to the lower end portion, but is not limited to this if it is within a range smaller than the second inner diameter. .

In addition, the lower part according to the present invention is connected to the middle part, the inside is hollow in a cylindrical shape, and 1 to 50 pores with a diameter of 0.1 mm are drilled in the end section. The spheroid or organoid is cultured at the pipette tip when replacing the medium in an experiment using a spheroid or organoid sample generated by 3D cell culture due to the hole formed in the end cross section. It can be effectively prevented from coming up from the medium with a pipette tip and losing the cell sample.

As described above, the pipette tip for preventing sample loss according to the present invention, which includes the upper part, the middle part, and the lower part, may have a total length of 10 mm to 30 mm, and preferably 20 mm, but is not limited thereto.

In the present invention, the material of the pipette tip may be plastic, and the plastic is not limited to this, but polyethylene (polyethylene, PE), polypropylene (polypropylene, PP), polystyrene (polystyrene, PS), polyethylene terephthalate (polyethylene terephthalate, PET), polyamides (PA), polyester (PES), polyvinyl chloride (PVC), polyurethane (polyurethanes, PU), polycarbonate (PC), poly Polyvinylidene chloride (PVDC), polytetrafluoroethylene (PTFE), polyetheretherketone (polyetheretherketone, PEEK) and polyetherimide (polyetherimide, PEI) may be used. Preferably, a polypropylene (PP) material may be used to mold the entire mold.

The pipette tip for preventing the loss of a sample devised in the present invention can be usefully used for the following applications (see FIG. 5).

It can be used to replace the medium in a 3D cell culture using tissue, spheroid or organoid as a sample. When using an existing tip, the sample is sucked up into the tip in the process of removing the culture medium with the tip, resulting in loss of the sample. However, the pipette tip of the present invention has a hole of 0.1mm to prevent loss of the sample.

In addition, the pipette tip of the present invention can be used for washing a sample using a tip. The sample can be inserted into the pipette tip and passed through a medium or a washing solution through a hole at the bottom to perform a washing process without losing the sample in the pipette tip. have.

In addition, the pipette tip of the present invention can be used for the purpose of moving the cleared tissue. When performing the clearing process in a microscopic observation experiment through staining of tissues, spheroids or organoids, the samples are cleared and not easily visible. Since there is a problem in that a large amount is lost when the sample is moved using the existing tip, the pipette tip of the present invention has little sample loss even for the transparent sample.

Therefore, the pipette tip for preventing sample loss of the present invention is difficult to handle with a conventional tip and can minimize the loss of a sample for easily lost cell samples, leading to a more accurate experiment result, and the sample loss of the present invention The pipette tip for prevention can be manufactured in one piece by connecting the upper part, the middle part and the lower part by using one material, and thus it is possible to mass-produce at a low price, which is advantageous economically.

So far, the present invention has been focused on the preferred embodiments. Those skilled in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered in terms of explanation, not limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent range should be interpreted as being included in the present invention.

Claims (7)

An upper portion of which the inside is cylindrical and empty as a site attached to the pipette;
A middle part connected to the upper part and having an empty inside in a form of a combination of two cut cones; And
It is connected to the middle portion and the inside is cylindrically hollow, and includes a lower end with 1 to 50 pores having a diameter of 0.1 mm on the end section.
Pipette tips to prevent sample loss. According to claim 1,
The sample is a tissue (sissue), a spheroid (spheroid), or an organoid (organoid), characterized in that the sample loss prevention pipette tip (tip). According to claim 1,
The upper portion is detachable with the pipette, the outer diameter is 5mm to 7mm, the first inner diameter is characterized in that 3.5mm to 4.5mm, pipette tip for preventing sample loss (tip). According to claim 1,
A pipette tip for preventing sample loss, characterized in that the second inner diameter of the middle part is 2.5 mm to 3.4 mm, and the third inner diameter is 0.5 mm to 2.4 mm. According to claim 1,
The pipette tip is characterized in that the total length is 10mm to 30mm, the pipette tip for preventing sample loss (tip). According to claim 1,
The pipette tip, characterized in that made of plastic, a pipette tip (tip) for preventing sample loss. The method of claim 6,
The plastic is polyethylene (PE), polypropylene (polypropylene, PP), polystyrene (polystyrene, PS), polyethylene terephthalate (polyethylene terephthalate, PET), polyamides (polyamides, PA), polyester (polyester, PES) , Polyvinyl chloride (PVC), polyurethane (polyurethanes, PU), polycarbonate (PC), polyvinylidene chloride (PVDC), polytetrafluoroethylene (PTFE), polyether A pipette tip for preventing sample loss, characterized in that it is selected from the group consisting of polyetheretherrketone (PEEK) and polyetherimide (PEIK).

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