KR101785441B1 - Improved pipette tip - Google Patents

Improved pipette tip Download PDF

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Publication number
KR101785441B1
KR101785441B1 KR1020150035857A KR20150035857A KR101785441B1 KR 101785441 B1 KR101785441 B1 KR 101785441B1 KR 1020150035857 A KR1020150035857 A KR 1020150035857A KR 20150035857 A KR20150035857 A KR 20150035857A KR 101785441 B1 KR101785441 B1 KR 101785441B1
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KR
South Korea
Prior art keywords
pipette tip
filter
ribs
mounting shaft
upper body
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Application number
KR1020150035857A
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Korean (ko)
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KR20160111120A (en
Inventor
박용성
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주식회사 르메디
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Priority to KR1020150035857A priority Critical patent/KR101785441B1/en
Publication of KR20160111120A publication Critical patent/KR20160111120A/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/0275Interchangeable or disposable dispensing tips
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/021Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/022Capillary pipettes, i.e. having very small bore

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

An improved pipette tip is disclosed that meters a microliter range of liquid mounted on a mounting shaft at the tip of a micropipette. In order to prevent the pressure loss of the air from being mounted on the mounting shaft, at least one annular wrinkle is formed in the upper part of the upper part, and a conical shape is formed in the lower part of the upper part. And a lower body formed with a graduation for the pipette tip. According to the present invention, the structure of the pipette tip can be improved to improve the durability, accuracy, and user's convenience.

Description

Improved pipette tips {IMPROVED PIPETTE TIP}

The present invention relates to an improved pipette tip, and more particularly to an improved pipette tip which is mounted on a micropipette for metering a small amount of liquid, thereby improving the durability, precision, ≪ / RTI >

Micro-pipettes are the basic device used to sample or transfer small quantities of samples in the chemical, life sciences, and medical fields. It is widely used in all areas where a relatively small amount (e.g. microliter range) of liquid, such as in molecular biology, has to be weighed. Once the pipette is used, the sample will remain on the inside of the pipette, leaving a very small amount. Therefore, reuse is prohibited in order to ensure the stability of the precision analysis test. Therefore, a disposable pipette tip, which can be replaced at the tip of the pipette, is used as an improvement measure.

The micropipette may be in the form of a pipetting unit or a pipetting robot or a manual pipetting unit or a pipetting machine having a plurality of individual pipetting units operating simultaneously or separately.

The basic mode of operation of the micropipette is based on the fact that a limited volume of fluid is displaced, for example, in a cylinder. The cylinder is in this case the outlet side hermetically connected to the through opening of the pipette tip so that the volume displacement of the fluid sucks the medium of the corresponding volume to be pipetted at the pointed end of the pipette tip. To ensure accurate weighing, the pipette tip must be securely and firmly connected to the micropipette. This is further needed if the secure fit of the individual pipette tips and precise positioning can not be manually checked.

In general, pipette tips are classified into three products according to capacity, and various products with special functionality are also sold and used. At present, three kinds of standard micropipettes (10 μl, 200 μl, and 1000 μl) account for about 60% of the total market and about 40% of functional products.

Functional products include low-binding tips and filter-tipped tips that prevent samples from remaining on the inner walls of the tip, and are expected to account for 70% of these functional products in future markets .

1 is a view for explaining a conventional pipette tip. Referring to Fig. 1, the disposable pipette tip 20 is fitted to a mounting shaft 30 at the tip of the micropipette. The sample is sucked and dispensed through the pipette tip 20 by pushing and releasing the suction / dispensing button 14. The ejector arm 26 is lowered when the tip ejector 24 is depressed so that the sleeve 28 fitted to the mounting shaft 30 is lowered and the pipette tip 20 is pushed downward to push down the pipette tip 20 Separation takes place.

The conventional pipette tip 20 may be damaged or deformed while receiving a strong force when the pipette tip 20 is mounted on the mounting shaft 30 or an error may occur due to air pressure loss. That is, since the pipette tip 20 is fitted to the mounting shaft 30 in a forced fit manner, the pipette tip 20 is subjected to an unstable force (or a very strong force in some cases) And changes. In addition, when the filter is applied, the filter is not stably mounted inside the pipette tip 20, so that the filtering is not properly performed.

Korean Patent Publication No. 10-2013-0124373 (published on November 13, 2013) Korean Registered Utility Model No. 20-0302951 (registered on January 20, 2003) Korean Registered Utility Model No. 20-0344817 (Registered on Mar. 3, 2004)

Accordingly, an object of the present invention is to provide an improved pipette tip having a structure that can be stably installed inside a filter without causing damage or distortion of the filter even when a strong force is applied thereto, without causing air pressure loss.

In order to achieve the object of the present invention, a pipette tip mounted on a mounting shaft at the tip of a micropipette of the present invention for metering a microliter range of liquid prevents pressure loss of the air when mounted on the mounting shaft And a lower body formed in a conical shape extending from the upper body and becoming narrower toward the lower side and having graduations for liquid metering formed on the outer side thereof.

A plurality of ribs are formed on the outer side of the upper body such that the ribs extend in a straight line from the upper portion to the lower portion and the lower ends of the plurality of ribs are formed in a vertical shape.

A filter fixing jaw for fixing the filter is formed in the upper body, and a plurality of reinforcing ribs extending from the plurality of ribs may be formed on the upper body.

The at least one annular wrinkle may have different heights.

According to the improved pipette tip of the present invention, since the annular wrinkle seals the space between the mounting shaft and the mounting shaft, it is possible to prevent pressure loss of the air. Therefore, when measuring the liquid, Or leakage of the liquid can be prevented, so that it is possible to measure an accurate amount of liquid without error.

Further, when the pipette tip is mounted on the mounting shaft, the pipette tip can be prevented from being slid and rotated by the plurality of ribs, and the lower end of the plurality of ribs can be supported by hand, It can be mounted accurately and easily on the shaft.

In addition, a filter fixing step may be formed on the upper body mounted on the mounting shaft of the micropipette so as to prevent the filter from being pressurized and to prevent the filter from being tilted, thereby maintaining the performance of the filter.

And the height of the corrugations can be made different from each other, so that the prevention of air pressure loss can be further strengthened.

1 is a view for explaining a conventional pipette tip,
FIG. 2 is a perspective view illustrating a structure of a pipette tip according to an embodiment of the present invention. FIG.
3 is a cross-sectional view illustrating a structure of a pipette tip according to an embodiment of the present invention,
4 is a perspective view illustrating a structure of a pipette tip according to another embodiment of the present invention, and FIG.
5 is a cross-sectional view illustrating a structure of a pipette tip according to another embodiment of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS The above and other features and advantages of the present invention will become more apparent from the following detailed description of the present invention when taken in conjunction with the accompanying drawings, It will be possible. The present invention is capable of various modifications and various forms, and specific embodiments are illustrated in the drawings and described in detail in the text. It should be understood, however, that the invention is not intended to be limited to the particular forms disclosed, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the terms "comprising" or "having ", and the like, are intended to specify the presence of stated features, integers, steps, operations, elements, parts, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, parts, or combinations thereof.

The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component.

Hereinafter, an improved pipette tip (hereinafter, referred to as a 'pipette tip') according to a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 2 is a perspective view illustrating a structure of a pipette tip according to an embodiment of the present invention, and FIG. 3 is a cross-sectional view illustrating a structure of a pipette tip according to an embodiment of the present invention.

The pipette tip 100 is mounted on a mounting shaft at the tip of a micropipette to meter a microliter range of liquid. FIGS. 2 and 3 show a pipette tip 100 used for 200 μl, and are divided into an upper body 110 and a lower body 160.

When the upper body 110 is mounted on the mounting shaft of the micropipette, two wrinkles 120 and 125 for preventing the pressure loss of the air are formed inside the upper portion. That is, the annular wrinkles 120 and 125 are formed so as to protrude inwardly of the pipette tip 100 so as to seal the mounting shaft, thereby preventing pressure loss of the air. Therefore, when weighing the liquid, it is possible to prevent the inflow or outflow of air through the portion mounted on the mounting shaft, so that an accurate amount of liquid can be weighed without any error.

Here, the height of the two corrugations 120 and 125 may be different from each other to prevent the pressure loss of the air more strongly. For example, when the inclination of the inner wall of the upper body 110 is smaller than the inclination of the outer wall of the mounting shaft, the height of the second corrugation 125 is higher than that of the first corrugation 120, . On the contrary, when the slope of the inner wall of the upper body 110 is larger than the slope of the outer wall of the mounting shaft, the height of the second corrugations 125 is formed lower than the first corrugations 120.

Meanwhile, when the pipette tip 100 is formed, when the wrinkles 120 and 125 are formed, the pipette tip 100 is prevented from completely coming into close contact with the mold, and the pipette tip 100 can be easily separated from the mold. .

A plurality of ribs 130 are formed on the outer side of the upper body 110 in a straight line from top to bottom. Here, the plurality of ribs 130 are formed to extend in height from the upper portion to the lower portion, and are formed vertically at the lower end. When the pipette tip 100 is mounted on the mounting shaft by the plurality of ribs 130, the resistance against external strong force is improved, and the pipette tip 100 can be prevented from being damaged or deformed.

That is, when a plurality of ribs 130 are not formed, the ribs 130 may be bent or pressed when receiving strong force in one direction. However, the plurality of ribs 130 formed on the outer wall of the upper body 110 prevent can do. In addition, when the pipette tip 100 is mounted on the mounting shaft, the pipette tip 100 can be prevented from being slid and rotated by the plurality of ribs 130 by the hand, The pipette tip 100 can be accurately and easily mounted on the mounting shaft.

It is also possible to prevent the external surface of the pipette tip 100 from being scratched during the automated process of packaging the product by the plurality of ribs 130, (100) is moved in a line by shaking to be mounted on the tip box.

A filter fixing jaw 140 for fixing the filter 150 is formed on the inner side of the upper body 110. Generally, when measuring the DNA, RNA, and various microbes by using the pipette tip 100, the micropipette may be contaminated by the aerosol evaporated from the liquid contained in the pipette tip 100. To prevent this, Filter 150 is used. Such a filter 150 is formed of a rigid polymeric resin, but may lose performance when pressure is applied.

That is, in the past, the filter 150 was pushed into the pipette tip 100 without an adhesive, and the filter 150 was fitted to an arbitrary portion without interference. In this case, pressure may be applied to the filter 150 and the filter 150 may be broken and the filter 150 may lose its function as a filter. 3, a filter fixing jaw 140 is formed at a boundary portion between the upper body 110 and the lower body 120 so that the filter 150 can be stably mounted on the end portion of the mounting shaft.

As described above, the filter fixing step 140 is formed at the end of the upper body 110 mounted on the mounting shaft of the micropipette, so that the filter 150 is prevented from being pressurized by the interference fit, So that the performance of the filter can be maintained.

The lower body 160 is formed in a conical shape extending to the upper body 110 and becoming narrower toward the lower side. Scales 161, 163 and 165 for liquid measurement are formed on the outer side of the lower body 160 and scales 161, 163 and 165 are formed in 20 μl, 50 μl and 100 μl for the 200 μl pipette tip 100. Here, when the scale is formed from 10 μl, when the pipette tip 100 is immersed in the liquid for weighing the liquid, a droplet may be formed on the portion where the 10 μl scale is formed, which may cause a scale metering error. Therefore, this error can be reduced by forming from 20 눈 scale. Since the amount of liquid can be visually confirmed by these graduations 161, 163, and 165, it is possible to prevent errors that occur when the micropipette is broken or the pipette tip 100 is erroneously mounted. That is, if the micropipette fails or the pipette tip 100 is misplaced, the researcher may not be able to precisely inject and apply the solution required for the experiment, which may result in erroneous experimental results. Therefore, it is possible to precisely outline the liquid necessary for the experiment through the scale, thereby preventing the experimental error.

FIG. 4 is a perspective view illustrating a structure of a pipette tip according to another embodiment of the present invention, and FIG. 5 is a cross-sectional view illustrating a structure of a pipette tip according to another embodiment of the present invention. FIGS. 4 and 5 show a pipette tip 200 used for 10 μl, which is divided into an upper body 210 and a lower body 260.

The upper body 210 is mounted on the mounting shaft of the micropipette, and three wrinkles 220, 222 and 224 are formed inside the upper part to prevent pressure loss of air when the upper body 210 is mounted. That is, the annular wrinkles 220, 222, and 224 are formed to protrude to the inside of the pipette tip 200 so as to be hermetically sealed with the shaft, so that pressure loss of the air can be prevented. Therefore, when weighing the liquid, it is possible to prevent the inflow or outflow of air through the portion mounted on the mounting shaft, so that an accurate amount of liquid can be weighed without any error.

Here, the three corrugations 220, 222 and 224 are formed to have different heights. In the case of the pipette tip 200 illustrated in FIGS. 4 and 5, the uppermost wrinkle 220 is formed to be the highest, and the lower end thereof is formed to be lower. Therefore, all three corrugations 220, 222 and 224 are brought into close contact with the mounting shaft, thereby further enhancing the hermeticity when the pipette tip 200 is mounted on the mounting shaft of the micropipette. That is, since the inclination of the inner wall of the pipette tip 200 is larger than the inclination of the outer wall of the mounting shaft, the height of the corrugations 220, 222, and 224 is lowered toward the lower portion so that all three corrugations 220, 222 and 224 are brought into close contact with the outer wall of the mounting shaft.

Meanwhile, when the pipette tip 200 is formed, the pipette tip 200 can be prevented from coming into close contact with the mold when the wrinkles 220, 222 and 224 are formed, and the pipette tip 200 can be easily separated from the mold.

A plurality of ribs 230 are formed on the outer side of the upper body 210 in a straight line from top to bottom. In the case of the pipette tip 200 for 10 [micro] l, the plurality of ribs 230 are formed in two stages as shown in Fig. 4 and Fig. That is, in the case of the pipette tip 200 for 10 μl, since it is small in size, it is more likely to be damaged or deformed by strong external force. Accordingly, a plurality of reinforcing ribs 235 extending from the plurality of ribs 230 can be formed to compensate for the strong resistance against external forces.

Here, the plurality of ribs 230 are formed to extend in height from the upper portion to the lower portion, and the lower ends thereof are formed vertically to extend into the plurality of reinforcing ribs 235. As described above, since the plurality of reinforcing ribs 230 can be formed to extend the length of the upper body 210, it is easy for a user to hold the body by hand. Since the size of the pipette tip 200 for 10 [micro] l is small, the plurality of ribs 230 are formed to be elongated and elongated, and thus can be bent. By forming a plurality of reinforcing ribs 235, do.

When the pipette tip 200 is mounted on the mounting shaft by the plurality of ribs 230 and the plurality of reinforcing ribs 235, the resistance against external strong force is improved and the pipette tip 200 is damaged or deformed . In addition, when the pipette tip 200 is mounted on the mounting shaft by the plurality of ribs 230 and the plurality of reinforcing ribs 235, the pipette tip 200 can be prevented from slipping and rotating in the hand, 200) can be accurately mounted on the mounting shaft. In the automated process of packaging the product by the plurality of ribs 230 and the plurality of reinforcing ribs 235, scratching of the outer surface of the pipette tip 200 can be prevented, It is easy for each pipette tip 200 to move in a line in the packaging process.

A filter fixing jaw 240 for fixing the filter 250 is formed on the inner side of the upper body 210. The filter fixing jaw 240 is formed at the point where the plurality of ribs 230 and the plurality of reinforcing ribs 235 extend from the upper body 210 in the pipette tip 200 for 10 μl. Generally, when measuring the DNA, RNA, and various microbes by using the pipette tip 200, the micropipette may be contaminated by the aerosol evaporated from the liquid contained in the pipette tip 200. To prevent this, Filter 250 is used. Such a filter 250 is formed of a rigid polymeric resin, but may lose performance if pressure is applied.

That is, in the past, the filter 250 was inserted into the pipette tip 200 without any adhesive, and the filter 250 was fitted to an arbitrary portion. In this case, pressure may be applied to the filter 250, which may cause the filter 250 to break, or the filter 250 may be jammed and lose its function as a filter. 5, a filter fixing jaw 240 is formed at a boundary portion between the upper body 210 and the lower body 220 so that the filter 250 can be stably mounted on the end portion of the mounting shaft.

As described above, the filter fixing jaw 240 is formed on the upper body 210 mounted on the mounting shaft of the micropipette so that the filter 250 is prevented from being pressurized by the interference fit, To maintain the performance of the system.

The lower body 260 is formed in a conical shape extending to the upper body 210 and becoming narrower toward the lower side. At the bottom of the lower body 260, scales 261, 263, 265 for liquid metering are formed on the outside. In the case of the pipette tip 200 for 10 μl, graduations 261, 263, and 265 are formed in 2 μl, 5 μl, and 10 μl. When the pipette tip 100 is immersed in the liquid to meter the liquid, a droplet may be formed on the portion where the 1 눈 scale is formed, which may cause a scale metering error. Therefore, this error can be reduced by forming from 2 눈 scale. Since the amount of liquid can be visually confirmed by these graduations 261, 263, and 265, it is possible to prevent errors in which the micropipette is broken or the pipette tip 200 is erroneously mounted.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined in the appended claims. It can be understood that it is possible.

100, 200: pipette tip 110, 210:
120, 125, 220, 222, 224: pleats 130,
235: reinforcing ribs 140, 240: filter fixing jaw
150, 250: filter 160, 260: lower body
161, 163, 165, 261, 263,

Claims (5)

A lower portion of the upper body and a lower portion which is formed in a conical shape which is narrowed toward the lower portion and is formed on the outer side and which is provided with a graduation for liquid metering, is mounted on a mounting shaft at the tip of the micropipette, In a pipette tip comprising a body,
A plurality of ribs formed on the outer side of the upper body and formed in a shape in which a height is linearly extended from an upper portion to a lower portion and a lower end is formed in a vertical shape;
A plurality of reinforcing ribs extending from the plurality of ribs to extend the length of the upper body; And
At least two or more ring-shaped wrinkles are formed so as to protrude inwardly from the inside and have different heights, while the uppermost wrinkle is the highest, and the lower the wrinkles An upper body formed to be lower in height and formed at a point where a plurality of reinforcing ribs extend from the plurality of ribs for a filter fixing tack for fixing the filter therein.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200086091A (en) 2019-01-08 2020-07-16 재단법인대구경북과학기술원 Pipette tip for preventing loss of sample

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113661386A (en) * 2019-03-28 2021-11-16 弗雷德传感科技公司 Attachment device for a fluid dispensing assembly
WO2024119395A1 (en) * 2022-12-07 2024-06-13 信任生医股份有限公司 Micropipette tip, kit including micropipette tip, and use thereof in measuring liquid level

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2803809B2 (en) * 1995-03-24 1998-09-24 ベクトン・ディキンソン・アンド・カンパニー Pipette tips
JP2004529746A (en) * 2001-01-12 2004-09-30 ポレックス,コーポレーション Pipette tip for easy installation and removal from the pipette
JP6011102B2 (en) * 2011-08-30 2016-10-19 セイコーエプソン株式会社 Object posture estimation method

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR200302951Y1 (en) 2002-10-25 2003-02-05 주식회사 와이즈랩 Pipette for long type yellow tip
KR200344817Y1 (en) 2003-12-09 2004-03-10 김종빈 The pipette tip which has a scale
US8524170B2 (en) 2011-02-22 2013-09-03 Rainin Instrument, Llc Pipette and sealing tip

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2803809B2 (en) * 1995-03-24 1998-09-24 ベクトン・ディキンソン・アンド・カンパニー Pipette tips
JP2004529746A (en) * 2001-01-12 2004-09-30 ポレックス,コーポレーション Pipette tip for easy installation and removal from the pipette
JP6011102B2 (en) * 2011-08-30 2016-10-19 セイコーエプソン株式会社 Object posture estimation method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200086091A (en) 2019-01-08 2020-07-16 재단법인대구경북과학기술원 Pipette tip for preventing loss of sample

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