KR20200082554A - Composition containing extract of aronia for prevention and improvement of benign prostatic hyperplasia and method for producing the same - Google Patents

Abstract

The present invention relates to a composition for preventing and alleviating benign prostatic hyperplasia, including an aronia extract as an active ingredient, and a method for preparing the same. More particularly, the present invention provides a method for preparing a fermented and concentrated aronia composition, including the steps of: (a) carrying out juice extraction of aronia and pretreatment of the extracted juice; (b) extracting and concentrating the extracted juice to provide a high content of anthocyanin; (c) mixing the aronia subjected to juice extraction with the anthocyanin concentrate; (d) fermenting the mixed solution; (e) mixing the fermented aronia solution with side ingredients for preventing or alleviating benign prostatic hyperplasia; and (h) packaging the resultant product. The fermented and concentrated aronia composition has an effect of preventing and alleviating benign prostatic hyperplasia.

Description

Composition and extract of aronia for prevention and improvement of benign prostatic hyperplasia and method for producing the same}

The present invention relates to a prostate hyperplasia prevention and improvement composition comprising an aronia extract as an active ingredient and a method for manufacturing the same. More specifically (a) juice and pre-treatment of aronia; (b) extracting and concentrating the juice to contain a high content of anthocyanin; (c) mixing the juiced aronia and anthocyanin concentrate; (d) fermenting the mixed solution; (C) mixing the Aaronia fermentation solution and the subsidiary material; (h) provides a method for producing an aronia fermentation concentrate composition comprising the step of packaging the above product.

The Aaronia fermentation concentrate composition has an effect of preventing and improving prostatic hyperplasia.

The prostate is a chestnut-sized organ in which small glands are gathered like honeycombs, secreting sperm that nourish sperm and prevent infection of pathogens. The location is just under the bladder, and the urethra is wrapped in a donut shape, and the prostate grows from the second sexage to about 20g around 30 years old. Urine and semen are discharged through this area.

The causes of prostate disease are various causes such as obesity, family history, metabolic syndrome, and diet, and symptoms may include prostatitis, prostatic hyperplasia, and prostate cancer.

Among prostate diseases, prostatitis has a prevalence of 5% to 9% in adults, and 25% of outpatients in urology and about 15-25% of visiting urologists in Korea are prostate infection syndrome. . However, urologists encounter prostatitis patients very often, but the diagnosis and treatment effects are not satisfactory, and both the doctor and the patient who are treating them are in trouble.

Since prostatic hyperplasia is a disease caused by aging, the higher the age group, the higher the incidence of patients, and especially the incidence from the 50s.

Aronia melanocarpa, also known as black chokeberry, is a rosaceae fruit tree. It is a shrub native to North America. It generally has a height of 90-180 cm. Fruits are about 6 mm in diameter. It is forming a fault.

It was introduced to Russia in the 19th century and is now widely cultivated in Sweden, Poland, the Czech Republic, Slovakia and Ukraine. Currently, mainly cultivated varieties include'Viking','Nero', and'Aron'. Aronia has a sour and astringent taste in fruits, so it is mainly used for processing rather than raw food.It contains a large amount of anthocyanin, so it can be used as a natural pigment added to foods such as jam and wine, as well as beverages such as juice and tea that promote antioxidant. It is widely used, and is particularly popular in Russia and Eastern Europe as a natural medicine for hypertension, arteriosclerosis, hydrochloric acid deficiency and vitamin deficiency.

As Aaronia recently launched the WTO system and the FTA entered into force, the world's agricultural products entered the era of endless competition, and one of the most active countermeasures to survive in the international competition and the opening of agricultural products is to specialize in high value-added agricultural products. At the same time, the development and commercialization of health-oriented processed foods using the functions of agricultural products are scientifically verified, widely distributed in domestic and foreign markets, and spread, thereby establishing a stable supply and demand system of our agricultural products and increasing the value of agricultural products. Realization is considered the best alternative

According to the current state of technology on Aaronia, in the paper of Aaronia's domestic journal, the effect of the addition of Aaronia powder on the antioxidant activity and quality characteristics of pork patties (Korean Journal of Food and Cookery Science, 2015, 31(1), 83-90), Quality characteristics of jelly added with Aronia melancocarpa juice (Korea Journal of Food and Cookery Science, 2015, 31(4), 456-464), optimization of fermentation conditions for the production of aronia vinegar using response surface method (Korea Food and Cookery Science Society , 2014, 30(6), 792-799), Antioxidant activity effect of aronia extract and aronia anthocyanin fraction (Korea Food and Cookery Science Society, 2014, 30(5), 573-578), Cheongpomuk with Aronia powder added Quality Characteristics and Antioxidant Activities of Korea (Korea Food and Cookery Science Society, 2014, 30(2), 161-169), Effect of Adding Aronia Powder on the Quality and Antioxidant Capacity of Muffins (Korea Food Storage and Distribution Society, 2014, 21(5) , 668-675), Physiological activity of aronia extract according to the extraction solvent (Korea Food Storage and Distribution Journal, 2014, 21(5), 718-726), quality characteristics of bread added with aronia powder (Korea Food and Nutrition Journal, 2014, 43(2), 273-280), anti-inflammatory effect of Aronia fruit hydrothermal extract on LPS-stimulated RAW 264.7 macrophages (Korea Food and Nutrition Science Society, 2015, 44(1), 7-13), Antioxidant and Antiallergic Efficacy of Extracts from Aronia (Aronia melanocarpa) (2008, 37(9), 1109-1113), Antioxidant Content and Antioxidant Activity of Aronia melancocarpa Hot Water Extract by Drying Method (Korea Food Science Journal, 2014, 46(3), 303-308), Stability of anthocyanin pigments from Aronia melancocarpa (Korea Food Science Society, 2013, 45(4), 416-421) Fermentation characteristics of makgeolli (Korea Food Science Society, 2016, 48(1), 27-35), reducing the tannin content of aronia using yeast isolated from salted fish Study on effects (Korean Society of Mycology, 2015, 43(4), 247-252), Enhancement of anti-inflammatory efficacy of aronia extract through fermentation process (Korean Journal of Medicinal Crop Science, 2014, 22(6), 475-482) , Comparison of wrinkle improvement efficacy of aronia extract according to extraction method (Korean Journal of Medicinal Crop Science, 2014, 22(3), 217-222), using various Leuconostoc mesenteroides strains in the development of Aronia alcohol extract for alternative natural substances Effect of Fermentation on Changes of Phenolic Compounds and Antioxidant Activities, Journal of Organic Agriculture, 2014, 22(4), 825-839), Quality Characteristics of Sulgidduk according to the Addition of Aronia Powder (East Asian Dietary Journal, 2014, 24(5 ), 646-653), and antioxidant activity of Aronia berry fruit and leaf extracts (Korean Journal of Cosmetics, 2013, 39(4), 337-345).

The thesis for aronia includes the effect of intake of aronia, montmorency, and plum extract on blood fatigue and muscle damage enzymes after endurance exercise (Shin Pan-jun, 2012), anthocyanin physiology isolated from Aronia melanocarpa Activity (Hyemi Park, 2014), Effect of suppression of rancidity of white chocolate ganache by adding Aaronia (Hyeran Park, 2014), Quality Characteristics and Consumer Preference of Turkish Delight with Aronia (Kim Han-hee, 2014), Vitamin C using liposomes Optimization of the capture process of Aronia concentrate and storage stability (Kwon Beom Kim, 2014), prostate specific antigen (PSA) expression study by blueberries and aronia in prostate cancer cell lines (Isolji, 2015), adding Aaronia Fermentation Characteristics of a Makgeolli (Mi-Jeong Park, 2015), Comparative Experimental Study on Domestic and Imported Product of Blackberry (Aronia) (Yeji Kim, 2015), Dyeing Characteristics of Hair Using Anthocyanin Pigment of Aronia melancocarpa (Kim Ju-young, 2015) ), Quality Characteristics and Antioxidant Activity of Sulgidduk with Aronia Powder (Hwang Young-lan, 2015), Physiological Activity Study of Aronia Extract as a Cosmetic Material (Shin Dong-hwa, 2015), Aronia Ingestion Adjuster during Maximum Exercise Using 2000m Rowing Machine Inflammatory Responses and Their Effects on Blood Fatigue Factors (Junghee Kim, 2016), The Effect of Temperature, Various Mother of Vinegar and Amount of Sugarin Chokeberry "Aronia melanocarpa" Vinegar Fermentation, Destiani Supeno, 2016), as DSS of Aronia berry extract Induced ulcerative colitis inhibitory effect (Gangsa-haeng, 2016).

Among prostate diseases, prostatitis has a prevalence of 5% to 9% in adults, and 25% of outpatients in urology and about 15-25% of visiting urologists in Korea are prostate infection syndrome. . However, urologists encounter prostatitis patients very often, but the diagnosis and treatment effects are not satisfactory, and there is a problem that both doctors and patients who are treated feel confused.

Therefore, in the present invention, to prepare a composition for preventing and improving prostatic hyperplasia, including a material that may have the efficacy of prostatic hyperplasia as an auxiliary material, while the aronia extract is contained in an amount of 50% or more so that the useful functionality of aronia can be sufficiently expected. Aim.

In order to solve the above problems, the present invention provides a fermentation-concentrating composition containing at least 50% of an aronia extract capable of sufficiently expecting useful functionality of aronia.

In order to solve the above problems, the present invention provides a method for preparing an aronia fermentation concentrate composition for preventing and improving prostatic hyperplasia, which includes a material that may have prostatic hypertrophy efficacy as an accessory material.

The present invention is anthocyanin content is high and contains more than 50% of the aronia extract and contains a material having an effect on prostatic hyperplasia as an additive, it can help in the prevention and improvement of prostate hyperplasia.

Figure 1. Shown is a process diagram of a manufacturing method for preventing and improving prostatic hyperplasia comprising an aronia extract.
Figure 2. As an example, the prostatic hyperplasia effect (prostate weight) of an aronia extract was shown.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for explaining the present invention in more detail, according to the gist of the present invention, the scope of the present invention is not limited by these examples to those skilled in the art to which the present invention pertains. It will be self-evident.

The present invention relates to a prostate hyperplasia prevention and improvement composition comprising an aronia extract as an active ingredient and a method for manufacturing the same. More specifically (a) juice and pre-treatment of aronia; (b) extracting and concentrating the juice to contain a high content of anthocyanin; (c) mixing the juiced aronia and anthocyanin concentrate; (d) fermenting the mixed solution; (e) mixing the Aaronia fermentation solution and an accessory material; (h) provides a method for producing an aronia fermentation concentrate composition comprising the step of packaging the above product.

Preparation of juice and concentrate of aronia

The Aaronia juice was juiced from the Aronia fruit using a screw-type juicer, and about 1 ton of juice was obtained based on the Aronia fruit and 6 tons. 180 liters of the juice was sterilized (80°C for 50 minutes), and the aronia residue was removed through a cylindrical filter with a primary filtration device, and impurities were completely removed using a secondary sediment filter. Next, the temperature of the concentrator was set to 75 degrees, and the Aaronia juice (12-13 brix) was concentrated to obtain a final 5.6-fold concentrated 60brix concentrate.

Mix Aaronia Extract and Anthocyanin Concentrate

The mixing ratio of the aronia juice solution and the anthocyanin concentrate was performed in the ratio shown in Table 1 (Table 1).

Mixing ratio of aronia juice and anthocyanin concentrate Aaronia Juice Anthocyanin concentrate Sample 1 90 10 Sample 2 95 5

Fermentation of mixed liquor

For alcohol fermentation, the initial sugar content was diluted with aronia concentrate (60°brix) to 25°brix, inoculated with Saccharomyces cerevisiae, incubated at 28°C for 7 days, and alcohol fermented using the mother.

In addition, in order to ferment the Aronia acetic acid, the alcohol fermentation solution was inoculated with Acetobacter sp. acetic acid strain and cultured at 30°C at 200 rpm for 72 hours to be used as a seed, and the initial acidity was adjusted to 1% and then at 28°C at 300 rpm for 2 weeks. Aronia acetic acid fermentation was performed.

Mixing with subsidiary materials

The fermented aronia composition was mixed with prostate hypertrophy, such as materials having prevention and improvement efficacy. The subsidiary material was prepared by mixing a gujipon concentrate.

Mixing ratio of the fermented aronia composition and the subsidiary material Fermented Aaronia Composition Subsidiary materials (gujipon concentrate, goji berry concentrate, etc.) Sample 1 90 10 Sample 2 80 20

Sensory test of aronia extract and aronia fermentation concentrate composition

The Aronia fermentation-concentrated composition prepared by the above-described manufacturing method was used as an experimental sphere, and the results of the sensory test for color and taste were shown in Table 2 using the Aronia extract as a control.

In the above, the sensory test consisted of 25 panelists who were well trained with more than 3 years of sensory test experience in the food field and conducted a total of 4 times. The sensory test of color and taste determined through preliminary training (color, bitterness, sweetness, synthesis) For the taste), a 9-point palatability rating method was used.

In Table 2, the sensory test value for whiteness is a value rounded up from the second decimal place after dividing the total score of the panel numbers by the panel number, indicating that the lower the value, the brighter the value, and the darker the higher the value.

Sensory test of aronia extract and aronia fermentation concentrate composition Item Sensory test (color, taste, flavor) color bitter sweetness Preference Aaronia Extract 7.0 8.0 5.8 8.0 Aronia fermentation concentrate composition 8.0 6.5 6.5 9.5

Animal breeding method for aronia extract

(1) After surgical treatment, the wound is recovered after a recovery period of one week.

(2) Test substance administration: For the induction of prostate hypertrophy, 3mg/kg of Testosterone is administered subcutaneously every day for 6 weeks (SC), and the body weight is measured once a week for the same period, and the test substance corresponding to each group is determined based on body weight. It is administered orally once a day.

Blood collection and tissue collection

(1) The autopsy is performed 6 weeks after administration of the test substance and is fasted the day before euthanasia.

(2) Blood collection: Euthanize with isoflurane gas and collect approximately 6 ml of blood from the abdominal vein. The whole blood is placed in a serum separating tube, left at room temperature for 30 minutes, and then centrifuged at 3000 RPM for 10 minutes to separate the serum, half of which is subjected to blood biochemical tests, and the rest is stored at -72°C for hormone analysis.

(3) Tissue collection: After blood collection is complete, the bladder and the prostate are carefully extracted, the total weight is measured, and then the prostate lateral and ventral lobes are separated from the urethra and weighed. In the case of the bladder, the mosquito forcep is prevented from leaking to measure the urine in storage, and the weight is measured. Half of the separated prostate tissue is fixed to formalin only for the area required for slide production. The remaining tissue is stored at -72°C for hormone analysis.

BHT analysis

DHT measurement in prostate tissue was measured by ELASA assay, and the prostate homogenate was added to a phosphate buffer of pH7.4, 4 times the prostate weight, homogenated, centrifuged at 5,000 rpm for 10 minutes, and the supernatant was used for the experiment.

After completion of the experiment, the extracted prostate weight was corrected with body weight to calculate the Prostate Index, and DHT analysis was performed on frozen prostate tissue and serum. All of the T1 to T4 groups were aronia extracts, but T1 extracts showed a decrease in DHT in the tissues (FIG. 2).

Claims (2)

(a) juice and pretreatment of aronia; (b) extracting and concentrating the juice to contain a high content of anthocyanin; (c) mixing the juiced aronia and anthocyanin concentrate; (d) fermenting the mixed solution; (e) mixing the Aaronia fermentation solution and an accessory material; (h) Aronia fermentation concentrate composition comprising the step of packaging the above product Method for preparing the Aaronia fermentation concentrate composition of claim 1

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