KR20200061848A - Centrifugal separator vessel for extracting buffy coat having function of checking recycle of the same - Google Patents

Abstract

The present invention relates to a vessel used in a centrifugal separator for separating substances having different components or specific gravity by using centrifugal force. Specifically, the present invention relates to a centrifugal separator vessel for buffy coat extraction having a function of checking the reuse thereof, wherein the centrifugal separator vessel forms a blood mark made by blood permeating therein during centrifugation so that the centrifugal separator vessel once used cannot be recycled, and can prevent the reuse of the centrifugal separator vessel in advance by preventing the blood from being easily removed even through washing.

Description

Centrifugation container for buffy coat extraction that can be checked for reuse {CENTRIFUGAL SEPARATOR VESSEL FOR EXTRACTING BUFFY COAT HAVING FUNCTION OF CHECKING RECYCLE OF THE SAME}

The present invention relates to a container used in a centrifugal separator for separating substances of different components or specific gravity by using centrifugal force, and specifically, to infiltrate the blood during centrifugation so as not to reuse the centrifugal container once used. The present invention relates to a centrifugal container for buffy coat extraction, which is capable of confirming whether or not to reuse the centrifugal container in advance by forming a blood mark and preventing the blood from being easily removed through washing.

Blood carries oxygen taken from the lungs to tissue cells, carries carbon dioxide from the tissues to the lungs, releases it, carries nutrients absorbed from the digestive tract to organs or tissue cells, and expands unnecessary substances in the body as products of tissue decomposition. It is transported to and discharged out of the body. In addition, the hormone secreted from the endocrine gland is transported to the working organs and tissues, the body heat is uniformly distributed to maintain the body temperature constant, and other bacteria, foreign substances, etc. that have invaded the living body are destroyed and detoxified. It performs various functions.

This blood consists of red blood cells, white blood cells and platelets. Among these, platelets are mainly present in plasma, and plasma is divided into PRP (Platelet rich plasma) and PPP (Platelet poor plasma). In particular, PRP is implanted in pain areas, especially the knee rest, ligaments, and muscles. It is used for therapeutic purposes because it plays a role in helping generation.

This PRP is a blood separated by centrifugation of its own blood to remove red blood cells, and only platelets and a growth factor-rich buffy coat are concentrated. Platelets and growth factors are highly concentrated. Accordingly, there are 2-7 times more platelets than normal blood, and include platelet-derived growth factor (PDGF), transformed growth factor (TGF), and epithelial growth factor (EGF).

Because PRP has the function of reconstructing and regenerating damaged parts of blood vessels, it can be used as a simulants to promote skin regeneration by highly enriching growth factors or to promote tissue regeneration such as prolotherapy. Regeneration and regeneration of (ligaments, tendons) is effective for arthritis, chronic back pain, pelvic pain, and shoulder or knee ligament damage.

As described above, PRP has excellent tissue regeneration ability than conventional prolotherapy, so it shows a fast healing power and uses autologous blood, so there are few side effects or infections. In addition, the procedure using PRP uses the patient's own blood, so there is less risk and does not cause hypersensitivity reactions. The number of clinically useful platelets is around 1x10 ⁶ /µl, and the number of platelets that secrete growth factors is higher through the concentration process, so if PRP is separated from the patient's blood and injected, it helps to recover wounds through active secretion of growth factors. Will give.

1 is a view illustrating a PRP separation container according to the prior art, and FIG. 2 is a view showing a buffy coat centrifuged through the PRP separation container shown in FIG. 1.

1 and 2, the PRP separation vessel 10 according to the prior art has a body 11 divided into an upper liquid chamber 12 and a lower liquid chamber 13, and an upper portion covering the upper portion of the upper liquid chamber 12 The stopper 15 and the lower stopper 16 that seals the lower portion of the lower liquid chamber 13 and the outer circumferential surface rises in close contact with the inner circumferential surface of the lower liquid chamber 13 by the force applied upward, and the upper liquid chamber ( 12) a fluid passageway (14a) for dividing the lower liquid chamber (13) and communicating the upper liquid chamber (12) and the lower liquid chamber (13) to the central portion comprises a liquid collecting part (14) provided in a structure protruding upward. .

In FIG. 1, '17', which is not described, denotes a packing member, and '18' denotes a locking jaw that is caught in the centrifuge.

The method for extracting the buffy coat using the PRP separation container 10 is as follows.

The blood collected from the human body is injected into the lower liquid chamber 13 using a syringe, and then the PRP separation vessel 10 into which blood is injected is seated in a centrifuge to perform centrifugation. Thereafter, when the centrifugation is completed, the red blood cells 2, PRP, and plasma 3 are sequentially separated from the lower portion into the lower liquid chamber 13 by a specific gravity difference in three stages. At this time, the PRP includes a buffy coat 1 which is a boundary layer divided into a white layer formed of platelets and white blood cells. In order to extract the buffy coat (1) from the structure separated into three steps, the buffy coat (1) is raised to the liquid collecting part (14) using a lifting member, and then the buffy coat (1) is selectively sucked using a syringe. To extract.

However, the PRP separation container according to the prior art can primarily prevent the reuse of the separation container by applying a method of extracting the buffy coat by raising the bottom stopper 16, but the raised bottom for buffy coat extraction As the case in which the container is reused again by descending the stopper 16 is frequently required, a container having a new structure capable of easily preventing the reuse of the container once used can be easily required.

KR 10-1334087 B1, 2013. 11. 22. KR 10-2002-0053210 A, 2002. 07. 05. KR 10-2009-0114804 A, Nov. 4, 2009

Accordingly, the present invention has been proposed to solve the problems of the prior art, and has the following objectives.

First, the present invention forms a blood mark that allows the blood to penetrate deeply into the inside when centrifuged on the inner surface of the container so that the blood on the centrifugation is not easily removed even when artificially washing the container once used for reuse. Another object is to provide a centrifugal container for buffy coat extraction that can be checked whether the reuse of the centrifugation container can be easily visually checked to prevent reuse of the container in advance.

Second, according to the present invention, the manufacturing cost is reduced through the simplification of the structure due to the non-use of the lower stopper or the rising member by automatically positioning and aligning at the point where extraction of the buffy coat is easy at the same time as centrifugation without using a separate lower stopper or rising member. Another purpose is to provide a centrifuge container for buffy coat extraction that can be checked for reuse to lower the price competitiveness.

Third, in the present invention, when the syringe is inserted into the container to insert the buffy coat that is automatically separated at a specific point after centrifugation, the syringe and the buffy coat are automatically aligned at the same time as the syringe is inserted, so that the buffy coat is automatically aligned. Another object is to provide a centrifugal container capable of checking whether it is reused, which can provide work convenience during extraction.

Fourth, the present invention reinforces both sides of the liquid collecting chamber by forming symmetrical structures or radially flanks on the sides of the liquid collecting chamber formed with a relatively thin diameter compared to other parts, while the centrifugal force is transmitted uniformly, thereby damaging the liquid collecting chamber. Another object is to provide a centrifugal container capable of preventing or preventing re-use to provide stable centrifugation.

Fifth, the present invention is to extract the buffy coat using a syringe by making it easy to visually identify the position of the buffy coat automatically separated at a specific point by forming a buffy coat confirmation window having a thinner thickness than the other parts at a specific point after centrifugation. Another object is to provide a centrifugal container capable of checking whether it is reusable, which can provide convenience when working.

The present invention for achieving the above object is a lower liquid chamber; Upper liquid chamber; A liquid collecting chamber having a lower end integrally connected to an upper end of the lower liquid chamber, and an upper end integrally connected to a lower end of the upper liquid chamber to mutually connect the lower liquid chamber and the upper liquid chamber; A sealing plate coupled to a lower portion of the lower liquid chamber to seal the open lower portion of the lower liquid chamber, wherein a gap for a blood mark is formed so that the upper surface and the lower end of the lower liquid chamber are spaced apart at regular intervals; And it provides a centrifugal container for buffy coat extraction that can be checked for reuse, characterized in that it comprises a lid for sealing the top of the upper liquid chamber.

Preferably, the gap for the blood mark may be characterized in that it is formed at intervals of 1 mm to 3 mm so that the blood that has penetrated into the interior during centrifugation is not easily removed even by washing.

Preferably, the sealing plate is separately manufactured in a plate structure and then joined to the lower end of the lower liquid chamber through thermal fusion, and an upper portion is formed with a coupling protrusion protruding upward along the circumference, and at the lower portion of the lower liquid chamber. A coupling groove is formed in a structure recessed inward along the circumference, and the sealing plate is coupled to the lower liquid chamber by fitting the coupling protrusion into the coupling groove, and the thickness of the coupling protrusion is at the depth of the coupling groove. Compared to the above, the gap for the blood mark is formed between the upper surface of the sealing plate and the lower end of the lower liquid chamber in the state where the engaging projection is inserted into the engaging groove, so that blood permeates into the inside during centrifugation. Can be done with

As described above, the present invention can obtain the following effects.

First, according to the present invention, by forming a blood mark on the inner surface of the container to infiltrate the blood deep into the inside upon centrifugation, it is easy to visually check whether the centrifugation container is to be reused to pre-reuse the centrifugation container. To prevent.

Secondly, according to the present invention, a liquid collecting chamber for interconnecting the upper liquid chamber and the lower liquid chamber between the upper liquid chamber and the lower liquid chamber is formed to have a smaller diameter than the upper liquid chamber and the lower liquid chamber, and centrifugation is performed simultaneously with the centrifugation. Compared to the centrifugal container according to the prior art (a structure that raises the buffy coat to a desired position using a separate bottom stopper and a lifting member) by allowing the buffy coat to be automatically positioned and aligned at a specific point (the point where extraction is easy) By simplifying, manufacturing cost can be lowered to secure price competitiveness.

Third, according to the present invention, when the wing pieces are formed to be symmetrical to each other on both sides of the collecting chamber, or by radially forming at least three or more blade pieces, the collecting chamber formed with a relatively small diameter when centrifuged at high speed While preventing damage, the centrifugal force is uniformly transmitted to stably perform centrifugation to shorten the extraction time of the buffy coat, while improving the extraction efficiency of the buffy coat.

Fourth, according to the present invention, when the syringe is inserted into the container to extract the buffy coat automatically separated at a specific point after centrifugation, the syringe and the buffy coat are automatically aligned at the same time as the syringe is inserted. Buffy coat extraction is convenient.

Fifth, according to the present invention, by forming a buffy coat confirmation window capable of checking the buffy coat in the liquid collection chamber, the location of the automatically separated buffy coat at a specific point in the liquid collection chamber can be easily checked with the naked eye to extract the buffy coat. It can provide work convenience.

1 is a view showing to explain the PRP separation vessel according to the prior art.
FIG. 2 is a view showing a buffy coat centrifuged through the PRP separation vessel shown in FIG. 1.
3 is a view for explaining a centrifugal separation vessel for buffy coat extraction according to Example 1 of the present invention.
4 is a view showing a state in which the cap and the cap of the centrifugal separation vessel shown in FIG. 3 are separated from the vessel.
5 is an internal perspective view of the centrifugal separation vessel shown in FIG. 3;
6 is a view of the centrifugal separation vessel shown in FIG. 3 as viewed from the front.
7 is a view illustrating a method for extracting a buffy coat using a centrifugal separation vessel according to Example 1 of the present invention.
8 is a cross-sectional view for explaining a centrifugal separation vessel for buffy coat extraction according to Example 2 of the present invention.
9 is a view showing a centrifugal separation container for extracting buffy coat shown in FIG. 8;
10 is a view of the centrifugal separation vessel shown in FIG. 8 viewed from the top;
11 is a view of the centrifugal separation vessel shown in FIG. 8 viewed from below.

Hereinafter, the technical features of the present invention will be described in detail with reference to the accompanying drawings.

3 is a view illustrating a centrifugation vessel for buffy coat extraction according to an embodiment of the present invention, and FIG. 4 illustrates a state in which the cap and the cap of the centrifugation vessel shown in FIG. 3 are separated from the vessel FIG. 5 is an internal perspective view of the centrifugal container shown in FIG. 3, and FIG. 6 is a view of the centrifugal container shown in FIG. 3 as viewed from the front.

3 to 6, the centrifugal separation vessel 20 according to an embodiment of the present invention is made of a transparent or semi-transparent material, the lower liquid chamber 21, the upper liquid chamber 22, and the lower liquid chamber ( 21) and a liquid collecting chamber 23 for connecting the upper liquid chamber 22 up and down in communication. In addition, it is coupled to the upper end of the upper liquid chamber 22 further includes a lid 24 for sealing the upper liquid chamber 22.

As shown in FIG. 5, the lower liquid chamber 21 is formed with a blood mark 211 to allow blood to seep into the interior during centrifugation on at least one of the inner bottom surface or the inner surface.

The blood mark 211 is a groove structure having a predetermined depth, and may be formed in a circular shape, a polygonal shape, or various shapes (pattern/shape). Preferably, in the centrifugation process, the blood may be formed into a whirl or spiral shape that is bent in the rotational direction of the centrifuge so that the blood on the blood mark 211 is not easily removed during washing after the blood has penetrated therein.

Blood mark 211, for example, as shown in Figure 5, the central groove (211a) formed in the center of the inner bottom surface of the lower liquid chamber 21, and a plurality of expansion grooves formed to extend outwardly from the outer surface of the central groove (221a) (211b). At this time, the central groove 211a is formed in a circular or elliptical shape, and the expansion groove 211b communicates with the central groove 211a and may be formed in a triangular, spiral or streamline shape formed at regular intervals along the outer circumferential surface of the central groove 211a. have.

In addition, the blood mark 211 is not only made of vertical grooves that are dug vertically, but is inclined at a constant angle with respect to the inner bottom surface of the lower liquid chamber 21 so that blood flows inward by centrifugal force during centrifugation. It may also consist of grooves. Through this inclined groove, blood on the inside can be prevented from being easily removed during washing.

On the other hand, the blood mark 211, as shown in Figure 5, but is made of a groove-shaped intaglio, as an example, may be made of embossed protruding from the inner bottom surface of the lower liquid chamber 21.

The lower liquid chamber 21 and the upper liquid chamber 22 may have the same diameter. In this case, the upper liquid chamber 22 is located in the liquid collecting chamber 23 between the lower liquid chamber 21 and the upper liquid chamber 22 so that the buffy coat 1 separated in the centrifugation vessel 20 after centrifugation is automatically performed. It is formed so as to have a larger volume than the lower liquid chamber (21). Preferably, as shown in Fig. 6, the length (L1) between the upper end of the upper liquid chamber 22 and the center of the collecting chamber 23, and the length (L2) between the bottom of the lower liquid chamber 21 and the center of the collecting chamber 23 The ratio of liver is 5.5 to 6.5 (L1): 3.5 to 4.5 (L2).

In this way, the volume ratio of the upper liquid chamber 22 and the lower liquid chamber 21, that is, the length L1 between the upper end of the upper liquid chamber 22 and the center of the liquid collecting chamber 23, and the lower and lower portions of the lower liquid chamber 21 The reason that the ratio between the lengths (L2) between the centers of the liquid chambers (23) is 5.5 to 6.5 (L1): 3.5 to 4.5 (L2) is that the buffy coat (1) is automatically in the collecting chamber (23) after centrifugation. To be positioned.

As shown in FIG. 2, blood collected from the human body is separated into a structure in which red blood cells/PRP (buffy coat)/plasma are sequentially stacked in three stages after centrifugation. At this time, the volume ratio of red blood cells in the whole blood is about 40%, and the rest is occupied by PRP and plasma.

Accordingly, in the present invention, the total volume of the centrifugation vessel 10 is formed at 110% to 120% in the amount of blood to be centrifuged, wherein the volume ratio of the upper liquid chamber 22 and the lower liquid chamber 21 is It is formed to be approximately 5.5 to 6.5 (L1): 3.5 to 4.5 (L2). This allows the buffy coat (1) to be automatically placed in the liquid collection chamber (23) at the same time as centrifugation, so that the buffy coat can be used without using a separate bottom stopper or a rising member in order to move the location of the buffy coat after centrifugation. It can be placed in the liquid collection chamber 23 for easy extraction.

As shown in FIG. 3, the collecting chamber 23 is a connecting passage through which the blood injected into the centrifuge container 20 flows by integrally connecting the upper liquid chamber 22 and the lower liquid chamber 21 vertically, and the upper end thereof has a top end. It is connected to the lower end of the upper liquid chamber 22, and the lower end is integrally connected to the upper end of the lower liquid chamber 21.

The liquid collecting chamber 23 is formed to have a smaller diameter than the upper liquid chamber 22 and the lower liquid chamber 21. Since the amount of the buffy coat collected from the blood is a relatively small amount of approximately 1% of the total blood, it is desirable to form a small diameter of the liquid collecting chamber 23 in consideration of this.

However, when the diameter of the liquid collecting chamber 23 is too small, blood flow between the upper liquid chamber 22 and the lower liquid chamber 21 is not smooth, and thus the blood and the lower liquid chamber filled in the upper liquid chamber 22 during centrifugation. The centrifugal force applied to the blood filled in (21) is different from each other.

Because of this, it is difficult to uniformly centrifuge the blood in the centrifuge container 20, and thus there is a limit to improve the recovery rate of the buffy coat. As the centrifugation was performed, not only was the work inconvenient, but the centrifugation time was increased and the efficiency was reduced.

Thus, in the present invention, the liquid collecting chamber 23 is formed to have a diameter of 3 mm to 7 mm. It is preferably formed of 4.6 mm.

The reason is that when the diameter of the liquid collecting chamber 23 is less than 3 mm, as described above, uniform centrifugation is difficult and the separation rate of the buffy coat is lowered. When it exceeds 7mm, the recovery rate of the buffy coat may be lowered when red blood cells or plasma are mixed during extraction using a syringe as the buffy coat is separated into a relatively thin thickness in the collecting chamber 23 after centrifugation.

Indeed, when centrifugation is performed under the same conditions using a centrifugal container having a diameter of the collection chamber 23 of less than 3 mm, the recovery rate of the buffy coat is 10 compared to a normal centrifugation vessel (diameter of the collection chamber is 3 mm to 7 mm). It was confirmed that it was reduced by about %.

In addition, in the case of using a centrifugal container with a diameter of the collecting chamber 23 exceeding 7 mm, when the amount of blood to be centrifuged is small, for example, 15 cc, separated in the collecting chamber 23 Since the buffy coat layer is relatively thin compared to red blood cells and plasma, it was difficult to selectively extract the buffy coat using a syringe between them.

And, the collecting chamber 23 is formed in a diameter of about 1/4 to 1/3 of the diameter of the upper liquid chamber 22 and the lower liquid chamber 21 within a diameter of 3 mm to 7 mm, or the volume is 1/4. It forms about ~1/3. In addition, the boundary between the upper liquid chamber 22 and the liquid collecting chamber 23 and the lower liquid chamber 22 and the liquid collecting chamber 23 is formed to have a constant curvature for smooth flow of blood during centrifugation. It is preferably formed to have an angle of 60° to 85° at the boundary between them.

On the other hand, the collection chamber 23 may be formed in at least a portion of the buffy coat confirmation window 23a so that the buffy coat 1 automatically separated therein after centrifugation can be easily recognized by the naked eye.

The buffy coat confirmation window 23a may be formed in the entire collection chamber 23 or may be formed in a part. For example, the buffy coat confirmation window 23a may be formed to a height of 20 mm from the bottom of the liquid collection chamber 23.

When the collecting chamber 23 is made of the same material as the upper liquid chamber 22 and the lower liquid chamber 21, the buffy coat confirmation window 23a may be formed to have a thinner thickness than other parts to improve transparency.

In addition, the buffy coat confirmation window 23a may be formed in a convex shape (convex lens) protruding outward from the liquid collecting chamber 23. Through this, the buffy coat separated from the inside of the liquid collecting chamber 23 may be exposed to the outside in an enlarged state larger than the actual size due to the convex structure buffy coat confirmation window 23a.

In addition, the buffy coat confirmation window 23a may be made of a soft material so that at least a portion of the collection chamber 23 can be directly extracted by inserting a syringe.

The lid 24 is screwed to the upper end of the upper liquid chamber 22 to seal the upper portion of the upper liquid chamber 22.

In the center of the lid 24, an extraction hole 24a that is vertically aligned with the liquid collection chamber 23 is formed, and the extraction hole 24a is sealed by being coupled with a soft stopper 25 in a forced fit manner.

As described above, in the present invention, an extraction hole 24a that is vertically aligned with an upper end of the liquid collecting chamber 23 is formed in the center of the lid 24, and the extraction hole 24a is sealed with a soft stopper 25 By doing so, the buffy coat separated in the liquid collecting chamber 23 after centrifugation can be more easily extracted.

7 is a view illustrating a method for extracting a buffy coat using a centrifugal separation vessel according to Example 1 of the present invention.

Referring to FIG. 7, when centrifugation is performed after filling the centrifuge container 10 with blood, blood is separated in the order of red blood cells 2, buffy coat 1, and plasma 3 due to specific gravity difference (FIG. 2).

At this time, as the volume ratio of the upper liquid chamber 22 and the lower liquid chamber 21 corresponds to the ratio of red blood cells and plasma constituting blood, the ratio is approximately 6:4, and after the centrifugation, the buffy coat 1 has the upper liquid chamber ( 22) and the lower liquid chamber 21 is automatically aligned in the liquid collecting chamber 23.

Thereafter, after inserting the syringe 30 into the soft stopper 25, the syringe needle is drawn into the buffy coat 1 separated inside the collecting chamber 23 and sucked to extract the buffy coat 1 by suction.

At this time, the syringe 30 is vertically aligned with the collection chamber 23 while the needle of the syringe descends vertically only by a simple operation of inserting the syringe 30 into the plug 25 without removing the cap 25 from the lid. It can be drawn in and extract the buffy coat 1 more easily.

On the other hand, the blood mark 211 formed in the lower liquid chamber 21 after centrifugation is stained with blood during the centrifugation process, so it can be visually confirmed whether it is used later, so it cannot be reused.

8 is a view showing a cross-section for explaining a centrifugal separation vessel for buffy coat extraction according to Example 2 of the present invention, and FIG. 9 is a view showing a centrifugal separation vessel for buffy coat extraction illustrated in FIG. 10 is a view of the centrifuge container shown in FIG. 8 viewed from the top, and FIG. 11 is a view of the centrifuge container shown in FIG. 8 viewed from the bottom.

8 to 11, the centrifugal separation vessel 30 for extracting a buffy coat according to Example 2 of the present invention, like the centrifugal separation vessel 20 for extracting a buffy coat according to Example 1 of the present invention, It includes the liquid chamber 31, the upper liquid chamber 32, the liquid collecting chamber 33 and the lid 34.

The lower liquid chamber 31 is made of a structure in which the lower portion is opened, and is sealed by a sealing plate 35. At this time, the sealing plate 35 may be coupled to the lower liquid chamber 31 through a heat fusion method, for example.

The sealing plate 35 is separately manufactured into a disk structure and then joined to the lower liquid chamber 31 through thermal fusion, and an engaging projection 35a protruding upwards along the circumference is formed on the upper portion thereof.

In addition, a coupling groove 31a is formed in a lower portion of the lower liquid chamber 31 in a structure recessed inward along the circumference. At this time, the engaging projection (31a) of the sealing plate 35 is fitted into the engaging groove (31a) is coupled.

As shown in FIG. 8, the thickness t of the engaging projection 35a is such that a depth (t) of the engaging groove 31a is formed between the upper surface of the sealing plate 35 and the lower surface of the lower liquid chamber 31 to form a constant gap (g). Forms larger than d). For example, the thickness t of the engaging projection 35a is formed from 0.4 mm to 0.6 mm, preferably 0.5 mm, and the depth d of the engaging groove 31 a is 0.2 mm to 0.4 mm, preferably It is formed into 0.3 mm.

The gap g between the sealing plate 35 and the lower liquid chamber 31 is approximately 0.1 mm to 0.3 mm, and preferably 0.2 mm. As described above, the reason for forming the gap g between the sealing plate 35 and the lower liquid chamber 31 is to form the blood mark described in Example 1 of the present invention. That is, in the centrifugation process, the blood filled in the lower liquid chamber 31 penetrates into the gap (g) between the sealing plate 35 and the lower liquid chamber 31 so that the blood on washing is not easily removed.

In addition, the centrifugal separation vessel 30 for buffy coat extraction according to the second embodiment of the present invention further includes wing pieces 36 formed in symmetrical structures with each other on both sides of the collecting chamber 33. The wing piece 36 is formed in a plate shape protruding outward from both sides of the liquid collecting chamber 33.

These wing pieces 36 are formed in a symmetrical structure on both sides of the liquid collecting chamber 33 formed with a relatively small diameter compared to the upper liquid chamber 32 and the lower liquid chamber 31, thereby reinforcing the strength of both sides of the liquid collecting chamber 33. By doing so, it is possible to prevent damage such as a breakage of the liquid collecting chamber 33 during centrifugation.

In addition, the wing piece 36 is made of a symmetrical structure so that centrifugal force can be uniformly transmitted to the centrifugal separation container 30 during centrifugation to provide stable centrifugation.

The wing piece 36 has an inner end connected to both sides of the collecting chamber 33, and an outer end connected to the inner surface of the connecting pipe 37 formed to surround the upper liquid chamber 32 and the lower liquid chamber 31. do. At this time, the diameter of the connecting pipe 37 may be formed with the same diameter of the upper liquid chamber 32 and the lower liquid chamber 31.

On the other hand, a pair of wing pieces 36 is provided in a symmetrical structure on both sides of the liquid collecting chamber 33, but this is an example, and at least three or more may be formed radially at a constant angle.

As described above, the technical idea of the present invention has been specifically described in a preferred embodiment, but the above-described preferred embodiment is for the purpose of explanation and not for limitation. As such, a person skilled in the art will understand that various embodiments are possible through a combination of the embodiments of the present invention within the scope of the technical spirit of the present invention.

1: Buffy coat
2: red blood cells
3: plasma
20, 30: centrifuge container
21, 31: Lower liquid chamber
22, 32: Upper liquid chamber
23, 33: collection room
23a: Buffy coat confirmation window
24, 34: lid
25: stopper
30: syringe
35: sealing plate
35a: engaging projection
31a: Coupling groove
36: wing
37: connector
211: blood mark

Claims (3)

Lower liquid chamber;
Upper liquid chamber;
A liquid collecting chamber having a lower end integrally connected to an upper end of the lower liquid chamber, and an upper end integrally connected to a lower end of the upper liquid chamber to mutually connect the lower liquid chamber and the upper liquid chamber;
A sealing plate coupled to a lower portion of the lower liquid chamber to seal the open lower portion of the lower liquid chamber, wherein a gap for blood marks is formed so that the upper surface and the lower portion of the lower liquid chamber are spaced at regular intervals; And
A lid sealing the upper portion of the upper liquid chamber;
It characterized in that it comprises a centrifugal container for buffy coat extraction that can be checked for reuse.
According to claim 1,
The blood mark crevice is a centrifugal container for buffy coat extraction that can be checked for reuse, characterized in that it is formed at intervals of 1 mm to 3 mm so that the blood that has penetrated into the interior during centrifugation is not easily removed even after washing.
According to claim 1,
The sealing plate is separately manufactured in a plate structure and then bonded to the lower end of the lower liquid chamber through heat fusion, and an engaging projection protruding upward along the perimeter is formed on the upper portion of the lower liquid chamber,
In the lower part of the lower liquid chamber, a coupling groove is formed in a structure recessed inward along the circumference,
The sealing plate is coupled to the lower liquid chamber, the engaging projection is fitted into the inside of the engaging groove,
The thickness of the engaging projection is formed to be larger than the depth of the engaging groove, so that the blood flows into the inside during centrifugation between the upper surface of the sealing plate and the lower end of the lower liquid chamber in the state where the engaging projection is fitted into the engaging groove. The clearance for the blood mark is formed to lift,
A centrifugal container for extracting buffy coat, which can be checked for reuse.

Images