KR20200049466A - Method and system for reducing harmful generated-substances including benzopyrene of argricultural processed goods using cold plasma - Google Patents

Abstract

The present invention relates to a method and system for reducing harmful substances including benzopyrene in agricultural processed products by using cold plasma. According to one embodiment, the method for reducing harmful substances, including benzopyrene generated from high-temperature processing of agricultural processed products, comprises a harmful substance reduction step of performing a cold plasma treatment on agricultural products processed at high temperatures, or agricultural processed products obtained by high-temperature processing of agricultural products, so as to reduce harmful substances. In addition, a system for reducing harmful substances including benzopyrene in agricultural processed products by using cold plasma is proposed.

Description

METHOD AND SYSTEM FOR REDUCING HARMFUL GENERATED-SUBSTANCES INCLUDING BENZOPYRENE OF ARGRICULTURAL PROCESSED GOODS USING COLD PLASMA}

The present invention relates to a method and system for reducing harmful products, including benzopyrene, in agricultural products processed using low-temperature plasma. More specifically, the present invention relates to a method and system for reducing harmful products including benzopyrene in agricultural products processed using low temperature plasma, which reduces harmful products such as benzopyrene generated during high temperature processing of agricultural products using low temperature plasma.

The benzopyrene (Benzo (a) pyrene, B (a) P: 3,4-benzopyrene; 1,2-benzopyrene) belonging to the group of polycyclic aromatic hydrocarbons (PAHs) is known to have strong genotoxicity and carcinogenicity. ) Is classified as group 1 (carcinogen). The International Food Standards Committee, Codex and JECFA, also classify benzopyrene as a carcinogen and endocrine disruptor. The JECFA) stipulates that the benzopyrene content in foods is up to 10.0 ppb, and that in the EU, the benzopyrene content in oils and fats is up to 2.0 ppb.

Looking at the contribution of benzopyrene to human exposure, 5.6% of air, 0.6% of water, and 93.8% of food are known to be the highest contributing factors. Benzopyrene in food is a harmful product produced by pyrolysis and incomplete combustion of carbohydrates, proteins and fats during heating during cooking and processing. One of the harmful products, benzopyrene, is also carried out by smoke in the smoke process. In particular, fat-containing foods, edible oils (edible oils, refined olive oil, sunflower oil, sesame oil, perilla oil, etc.) and flames are in direct contact and are often generated in blackened areas.

In Korea, after the detection of benzopyrene in olive oil was confirmed in 2006, the Ministry of Food and Drug Safety and the manufacturer have voluntarily engaged in efforts to reduce benzopyrene. Since October 2007, the benzopyrene standard for all edible oils is 2.0 μg / kg (ppb). Is managed by. However, even after that, benzopyrene was detected as a social problem in the instant production of instant noodles in large companies in 2012 and red pepper seed oil in 2013, and as a result, verification of the content of harmful products such as benzopyrene in various foods and food ingredients The demand for the development of over-reducing technologies is increasing.

Plasma is an ionized gas called a fourth substance, and is a collection of charged particles that are electrically neutral with cations and electrons distributed at almost the same density. Plasma contains a lot of excited state ions and undergoes several stages of transition, and also generates ultraviolet rays. Ions and high-energy electrons contained in the plasma are highly reactive, have strong oxidizing power, and can be used for the decomposition of harmful substances such as air pollutants, pesticides, and endocrine disruptors as well as sterilization with the action of ultraviolet rays.

Plasma is divided into low temperature plasma (also called non-thermal plasma or non-thermal plasma) and high temperature plasma according to temperature. High temperature plasma cannot be applied to biological materials such as food due to high temperature, whereas low temperature plasma has low temperature. It can be applied to materials that are vulnerable to back heat. The low-temperature plasma can decompose various compounds by oxidation reaction and electron shock, including oxygen atom, ozone, OH-radical, N-radical, plasma electron, photon, and ultraviolet rays.

Republic of Korea Patent Publication No. 10-2018-0073589 (published on July 2, 2018) Republic of Korea Patent Publication No. 10-2017-0050258 (released May 11, 2017) Japanese Patent Application Publication No. 2012-085791 (released May 10, 2012)

The present invention is intended to be applied to the reduction of benzopyrene, a toxic compound produced during food processing by utilizing the characteristics of low temperature plasma, and is safe by reducing harmful products such as benzopyrene generated by high temperature processing of agricultural products using low temperature plasma. It intends to contribute to the promotion of national health and the development of the food industry through food supply.

In order to achieve the above object, in accordance with one aspect of the present invention, in a method for reducing harmful products, including benzopyrene produced by high-temperature processing of agricultural products, processing processed through high-temperature processing of agricultural products A method for reducing harmful products including benzopyrene of agricultural products processed products using low temperature plasma is proposed, which includes a step of reducing harmful products by performing a low temperature plasma treatment on the resultant product.

In this case, in one example, the high-temperature processing step of performing a high-temperature processing to cause the generation of harmful products, including benzopyrene, for agricultural products before the step of reducing harmful products may be further included.

In addition, in this case, in one example, the agricultural products belong to one of the seeds, grains, nuts, and soybeans, and the processing result is a direct result of high temperature processing or a further processing result to which processing is directly added. In the high-temperature processing step, the roasting treatment may be performed on the agricultural products.

At this time, in another example, the roasting process is performed in the process of producing raw materials for coffee or tea beverages from the agricultural products belonging to any one of the seeds, cereals, and from the agricultural products belonging to any one of the seeds, nuts, and soybeans. Or, it may be performed in the process of processing directly from agricultural products belonging to any one of seeds, grains, nuts, and beans to food or food ingredients.

In addition, in another example, the roasting process in the raw material manufacturing process of the coffee or tea beverage may be roasting of coffee beans or roasting for any one of barley, corn, and corn.

In addition, in one example, the roasting treatment as a preliminary step of milking milk is roasting on any one of sesame seeds, perilla seeds, rapeseed seeds, and sunflower seeds, and low-temperature plasma treatment may be performed on the result of the roasting treatment or milked oils. .

In addition, according to one example, the low-temperature plasma treatment is plasma by any one of decompression discharge plasma (LPDP), dielectric barrier discharge plasma (DBDP), corona discharge plasma jet (CDPJ), and intermittent corona discharge plasma jet (ICDPJ). It can be treatment.

In this case, in one example, any one of air, oxygen, and nitrogen may be used as the plasma generating gas in the step of reducing harmful products.

Next, in order to achieve the above object, according to one aspect of the present invention, in a system for reducing harmful products including benzopyrene produced by high-temperature processing of agricultural products, low temperature with respect to plasma-generated gas A plasma generator that generates plasma; A blower for supplying plasma-generated plasma gas from the plasma generator; A treatment chamber for receiving a result of a processing treatment that has undergone high-temperature processing treatment for agricultural products, but performing a low-temperature plasma indirect treatment using plasma gas supplied by a blower to reduce harmful products including benzopyrene; And a control unit for controlling low-temperature plasma indirect processing of the processing result in the production chamber of the low-temperature plasma through the plasma generator, blowing through the blower, and the processing chamber, and a system for reducing harmful products including benzopyrene of agricultural products processed using low-temperature plasma. This is proposed.

At this time, in one example, the agricultural products belong to one of seed, grain, nut, and soybean, and the system for reducing harmful products transfers the processing result into the processing chamber and discharges the processing result of low temperature plasma indirect processing. Further comprising a unit, the processing chamber may be provided with a passage for transferring and discharging by the transfer unit and an exhaust port for discharging gas in the chamber.

In addition, in this case, in one example, the system for reducing harmful products further includes a high-temperature processing unit for roasting agricultural products, and the transfer unit is processed for a direct product or a direct product processed by the high-temperature processing unit. The added processing result is transferred as the processing result, and the high-temperature processing unit is one of seed, nut, and bean in the pre-milking stage, and either of seed, grain or grain in the raw material manufacturing process of coffee or tea beverages. Alternatively, in the process of processing directly into food or food ingredients, agricultural products belonging to any one of seeds, grains, nuts, and beans may be roasted.

In addition, in one example, the result of processing is milk fat that has been milked through high-temperature processing treatment on agricultural products belonging to one of seed, nuts, and soybeans, and the processing chamber accommodates the oil and fat container containing the fat and oil, and the bottom of the oil and fat container. In the porous dispersion pipe is provided, the plasma gas supplied by the blower is discharged into the air bubbles into the oil and fat through the porous dispersion pipe, and low temperature plasma indirect treatment can be performed.

According to another example, the plasma generator is a low temperature by any one of a pressure-sensitive discharge plasma (LPDP), a dielectric barrier discharge plasma (DBDP), a corona discharge plasma jet (CDPJ), and an intermittent corona discharge plasma jet (ICDPJ). Plasma can be generated.

At this time, in one example, the plasma generator may generate a low temperature plasma in a manner using a dielectric barrier discharge plasma (DBDP), and may use any one of air, oxygen, and nitrogen as the plasma generating gas.

In addition, in another example, the plasma generator may generate plasma in a current range of 0.4 to 0.6 A using air as a plasma generating gas.

According to the present invention, it is possible to reduce harmful products such as benzopyrene generated by high temperature processing of agricultural products by utilizing low temperature plasma.

It is obvious that various effects not directly mentioned according to various embodiments of the present invention can be derived by those skilled in the art from various configurations according to embodiments of the present invention.

1 is a flowchart schematically showing a method for reducing harmful products, including benzopyrene, in agricultural products processed using low temperature plasma according to one embodiment of the present invention.
2 is a flowchart schematically showing a method for reducing harmful products, including benzopyrene, in agricultural products processed using low-temperature plasma according to another embodiment of the present invention.
3 is a flowchart schematically showing a method for reducing harmful products, including benzopyrene, in agricultural products using low-temperature plasma according to another embodiment of the present invention.
4A-B are flowcharts schematically illustrating a method for reducing harmful products, including benzopyrene, in agricultural products processed using a low-temperature plasma according to another embodiment of the present invention.
5 is a flowchart schematically showing a method for reducing harmful products, including benzopyrene, in agricultural products processed using low-temperature plasma according to another embodiment of the present invention.
6 is a block diagram schematically showing a system for reducing harmful products, including benzopyrene, in agricultural products processed using a low temperature plasma according to another embodiment of the present invention.
7A-C are block diagrams schematically showing a system for reducing harmful products, including benzopyrene, in agricultural products processed using a low-temperature plasma according to one embodiment of the present invention.
8 is a graph showing the benzopyrene decomposition power of LPDP for each plasma generating gas.
9 is a graph showing a benzopyrene decomposition pattern model by air-LPDP.
10 is a graph showing the effect of LPDP generation pressure on the benzopyrene decomposition power of air-LPDP.
11 is a graph showing the effect of power consumption on the benzopyrene decomposition power of air-LPDP.
12A-F are graphs showing the effects of electrode gap (EG) and current intensity on the benzopyrene decomposition power of DBDP.
13 is a graph showing a benzopyrene decomposition pattern model by DBDP.
14A-C are graphs showing the effect of discharge length (span length, SL) and current intensity on the benzopyrene decomposition power of CDPJ.
15A-C are graphs showing the effect of discharge distance and current intensity on the decomposition of ICDPJ to benzopyrene.
16A-D are graphs showing the residual rate of benzopyrene of roasted sesame seeds according to the treatment time for each plasma type.
17A-D are graphs showing the residual rate of benzopyrene of oil milked from roasted sesame seeds with different treatment times for each plasma type.
18a-b is a graph showing the residual benzopyrene content change in coffee according to the CDPJ treatment.
19a-b is a graph showing the change in residual benzopyrene content in coffee according to ICDPJ treatment.
20 is a graph showing the residual benzopyrene content change in barley according to CDPJ treatment.
21 is a graph showing the change in residual benzopyrene content in barley following ICDPJ treatment.
22 is a graph showing the benzopyrene decomposition effect of ADDAP according to the current intensity.
23A-D are graphs showing benzopyrene reduction of roasted sesame seeds by ADDAP treatment.
24A-D are graphs showing the effect of ADDAP treatment of roasted sesame seeds on the benzopyrene content of oil after milking.
25A-B are graphs showing changes in residual benzopyrene content in sesame oil and perilla oil according to ADDAP treatment.

Embodiments of the present invention for achieving the above-described problems will be described with reference to the accompanying drawings. In the present description, the same reference numerals refer to the same configuration, and additional descriptions may be omitted in order to promote understanding of the present invention to those skilled in the art.

It should be noted that although a singular expression is described in this specification, it may be used as a concept representing a plurality of components unless it is interpreted contrary to, or distinctly contradictory to, the concept of the invention. It should be understood that descriptions of 'comprising', 'having', 'having', 'comprising', and the like in this specification have the possibility or presence of one or more other components or combinations thereof.

[Method for reducing harmful products including benzopyrene in agricultural products processed using low-temperature plasma]

First, a method for reducing harmful products including benzopyrene of agricultural products processed products using a low-temperature plasma according to one aspect of the present invention will be described with reference to FIGS. 1 to 5. In addition, in understanding the method for reducing harmful products, including benzopyrene, of agricultural products processed products using a low temperature plasma according to one example of the present invention, as well as the descriptions described below, of agricultural products processed products using a low temperature plasma according to one example of the invention described below Reference may be made to embodiments of the system for reducing harmful products including benzopyrene and descriptions of FIGS. 6 to 7c.

1 is a flowchart schematically showing a method for reducing harmful products, including benzopyrene, of agricultural products processed using a low temperature plasma according to one embodiment of the present invention, and FIG. 2 is a low temperature plasma according to another embodiment of the present invention. FIG. 3 is a flowchart schematically showing a method for reducing harmful products including benzopyrene in a processed agricultural product, and FIG. 3 schematically illustrates a method for reducing harmful products including benzopyrene in a processed agricultural product using a low-temperature plasma according to another embodiment of the present invention. 4a-b is a flowchart schematically showing a method for reducing harmful products, including benzopyrene, in agricultural products processed using a low temperature plasma according to another embodiment of the present invention, and FIG. 5 is another one of the present invention. Farming Using Low Temperature Plasma According to Example A flow diagram schematically illustrating the creation of hazardous substance reducing method including benzopyrene of the work piece.

1 to 5, a method for reducing harmful products including benzopyrene of agricultural products processed products using low-temperature plasma according to one example is a method for reducing harmful products including benzopyrene produced by high-temperature processing of agricultural products. . For example, in one example, a method for reducing harmful products including benzopyrene in agricultural products is a method for reducing benzopyrene produced according to high temperature processing of agricultural products.

1 to 5, a method for reducing harmful products, including benzopyrene, in agricultural products processed products using low-temperature plasma includes steps for reducing harmful products (S300, S300 ', S300 ". Benzopyrene, one of the harmful products, is an agricultural product. For example, it can be produced during high temperature processing such as roasting seeds such as sesame or coffee, cereals such as barley, nuts such as peanuts, beans such as beans, etc. Hazardous products such as benzopyrene are harmful substances such as cancer. Therefore, it is necessary to reduce and implement a method for reducing harmful products including benzopyrene in the present invention.Agricultural products are products obtained by processing agricultural products, which can be obtained through primary processing of agricultural products, secondary processing, etc. At this time, the processing in the present invention includes physical or chemical processing beyond simple drying, for example, roasting, baking, powdering, pressing, extraction, etc. In other words, the processed agricultural product may be an intermediate product in a processed state or a final product subject to a transaction after processing is completed.

As suggested by the applicant in a separate application No. 10-2018-0165416, the harmful product produced by high-temperature processing of agricultural products or agricultural products may include acrylamide in addition to benzopyrene. The reduction technique of acrylamide is described in detail in Application No. 10-2018-0165416. The technical content of application 10-2018-0165416 may be included in the scope of the present invention.

In the harmful product reduction step (S300, S300 ', S300 "), a low-temperature plasma treatment is performed on the result of the processed product that has undergone high-temperature processing for agricultural products to reduce harmful products, such as benzopyrene.

At this time, referring to Figures 3, 4a to 4b, in one example, the agricultural products may be one of seed, cereals, nuts, and beans. For example, the seeds include oilseed seeds such as sesame, perilla, rapeseed (seed), sunflower (seed), flax (seed), and beverages and sweet seed oils such as coffee beans, bean sprouts, cacao beans, etc., and are limited to those listed. Does not work. Grains include, but are not limited to, barley, corn, rice, yulmu, buckwheat, rye, and the like. Nuts include, but are not limited to, peanuts, almonds, ginkgo, pine nuts, pistachios, walnuts, and the like. Soybeans include, but are not limited to, soybeans, kidney beans, peas, and lentils.

For example, agricultural products subject to reduction of harmful products are mainly used for oiling through a roasting process or used for beverages such as coffee or tea after a roasting process, or those used directly for food and beverage after roasting. .

For example, the processing result, which is the object of the low temperature plasma treatment, may be a direct result of a high temperature processing treatment, such as a roasting treatment, or a further processing result to which a processing treatment for a direct result is added. High-temperature processing of agricultural products such as roasted sesame seeds, roasted coffee beans, roasted rapeseed (seed), roasted sunflower (seed), roasted flax (seed), roasted flax seeds, roasted cacao beans, roasted Barley, roasted corn, and the like, but is not limited thereto. The additional processing result to which the processing for the direct result is added is a product to which a physical processing process such as powdering or compressing the direct result is added, for example, may be a powder of the direct result, oil oiled from the direct result, etc. , But is not limited to this.

In addition, referring to FIGS. 2, 3, and 4B, in one example, a method for reducing harmful products including benzopyrene in agricultural products processed using low temperature plasma is processed at a high temperature before the steps of reducing harmful products (S300, S300 ', S300 "). The treatment step (S100, S100 ') may further include: In the high-temperature processing step (S100, S100') it is possible to perform a high-temperature processing treatment that causes the production of harmful substances, such as benzopyrene, for agricultural products. For example, high-temperature processing such as stir-fry may generate harmful products including benzopyrene.

Referring to FIGS. 3 to 4B, in one example, a roasting treatment may be performed as a high-temperature processing treatment on one of agricultural products, such as seed, cereals, nuts, and beans (S100 ').

At this time, in one example, in the high-temperature processing step (S100, S100 '), the roasting process is from the agricultural products belonging to any of the seeds, nuts, and soybeans. It can be carried out in the process of manufacturing raw materials for coffee or tea beverages, or in the process of processing directly from agricultural products belonging to any one of seeds, grains, nuts, and beans to food or food materials.

For example, the agricultural products that are roasted in the previous stage of milking milk include sesame, perilla, rapeseed, sunflower, flax, and peanuts belonging to nuts and soybeans belonging to soybeans. Compressed oil can be obtained by squeezing mainly roasted sesame seeds, perilla seeds, rapeseed, sunflower, and flax. In addition, the agricultural products that are roasted during the production process of raw materials for coffee or tea beverages include coffee belonging to seed species, barley seeds, and barley, corn belonging to grains. In addition, the agricultural products that are roasted in the process of direct food or food ingredients are sesame, perilla, sunflower, flax, etc. belonging to the species, barley, yulmu, etc. belonging to the grain, peanuts, almonds, ginkgo, pine nuts belonging to the nuts, There are pistachios, walnuts, and soybeans.

At this time, in one example, the roasting process in the raw material manufacturing process of the coffee or tea beverage may be roasting of coffee beans or roasting for any one of barley, corn, or corn.

In addition, in one example, the roasting process as a pre-step of milking milk fat may be roasting on any one of sesame, perilla, rapeseed, and sunflower seeds. For example, at this time, the low-temperature plasma treatment may be performed on the roasted product or may be performed on the milk fat milked from the roasted product. In one example, at this time, the low temperature plasma treatment may be performed on the stir-processed product or milked fats and oils.

In addition, referring to FIGS. 4A to 4B, in one example, the low temperature plasma treatment includes reduced pressure discharge plasma (LPDP), dielectric barrier discharge plasma (DBDP), corona discharge plasma jet (CDPJ), and intermittent corona discharge plasma jet (ICDPJ). ) May be a low-temperature plasma treatment. The specific method of the plasma treatment by each method will be referred to the embodiment described below.

In addition, referring to FIG. 5, in one example, one of air, oxygen, and nitrogen may be used as the plasma generating gas in the harmful product reduction step (S300 "). The harmful product reduction step (S300" of FIG. 5) ) May be applied to other drawings not shown.

Although the following examples show the results of experiments on benzopyrene produced during the roasting process of sesame, coffee, and barley, it is expected to reduce the low-temperature plasma treatment of harmful products other than benzopyrene produced by high temperature processing. Can be. In the applicant's application No. 10-2018-0165416, embodiments are proposed for reducing acrylamide harmful products generated in a frying process, which is a high-temperature processing process such as french fries, through low-temperature plasma treatment.

[Example-Roasted Sesame]

1) Sample preparation for plasma treatment

The benzopyrene decomposition power according to the operating conditions for each plasma type was investigated through benzopyrene analysis before and after treatment. Dispense 10 μl of a 20 ppm benzopyrene (benzo [a] pyrene, Sigma-Aldrich Co., St. Louis, MO, USA) solution (in ACN) into a slide glass, dry it in a hood for 30 minutes, and then treat plasma conditions for each type. Was treated.

To investigate the amount of benzopyrene produced by the roasting process and the reduction of benzopyrene by plasma, sesame and perilla samples were fried under the following conditions. Combustion is performed by supplying heat by burning propane gas, and when the sample reaches a certain temperature, the sesame and perilla are roasted under each condition using a commercial gas-fired roasting device that blocks heat and forces forced cooling. Treatment.

Typically, the roasted samples are called regular-roasted sesame (RRS) and regular-roasted perilla (RRP) until they reach the final roasting temperature (220 ° C of sesame, 210 ° C of sesame) applied for commercial purposes in commercial facilities. Roasted samples until they reach the high temperature at which smoke occurs (245 ° C sesame, 230 ° C perilla) were named over-roasted sesame (ORS) and over-roasted perilla (ORP), respectively.

2) Analysis of benzopyrene

The benzopyrene on the plasma-treated slide glass was dissolved in acetonitrile (ACN) 20 times in total 10 times, collected in a 1.5 mL Effendorf tube, and ACN was evaporated in a hood. Then, ACN 1.0 mL was added again to dissolve the dried benzopyrene, filtered through a 0.22 μm filter (Smartpor-II, PVDF syringe filter), sealed in a 2.0 mL vial, and then analyzed by HPLC using the method of Chen et al. (2012).

The sesame samples treated by plasma type are pulverized with a small grinder (Philips, HR 2860), 10 g of the sample is added, 100 mL of ethyl ether is added, oil and fat is extracted for 12 hours, and ethyl ether is evaporated and removed, and then 100 mL of hexane is Putting Whatman No. 2 The filtrate filtered with filter paper was transferred to a separatory funnel (I), and 50 mL of N, N-dimethylformamide-water (9: 1, v / v) was added, shaken vigorously, and left to stand. Thereafter, the N, N-dimethylformamide-water layer was transferred to another separatory funnel (II). Again, 25 mL of N, N-dimethylformamide-water was added to the hexane layer of the separating funnel (I) and allowed to shake, and the operation of combining the N, N-dimethylformamide-water layer with the separating funnel (II) was repeated twice. 100 mL of 1% Na ₂ SO ₄ solution and 50 mL of hexane were added to the N, N-dimethylformamide-water layer collected in the separatory funnel (II), stirred and allowed to stand, and then the hexane layer was transferred to another separatory funnel (III). After adding 35 mL of hexane to the N, N-dimethylformamide-water layer of the separatory funnel (II) and mixing, the operation of combining the hexane layer with the above separatory funnel (III) was repeated twice. Then, add 50 mL of distilled water to the separatory funnel (III), shake and mix, and then repeat the operation of discarding the distilled water layer twice, and then put the hexane layer on the floor about 15 g of anhydrous Na ₂ SO ₄ about 15 g. 2 Dehydrated using filter paper and concentrated under reduced pressure in a 40 ° C. water bath to about 2 mL.

Purification of the sample was performed using a Sep-Pak Florisil cartridge (3 cc, Waters Corp., Milford, MA, U.S.A.). The cartridge used for purification was used after distilling 20 mL of hexane and 10 mL of dichloromethane at a rate of 2-3 drops per second. After mixing the sample concentrate and 10 mL of hexane, the cartridge was passed through at a rate of 2-3 drops per second, and then 8 mL of hexane / dichloromethane (3: 1) was added to elute again. The eluate was concentrated again on a 40 ° C. water bath, and the residue was dissolved in 1 mL of ACN, filtered through a 0.45 μm membrane filter, sealed in a 2.0 mL vial, and used as an HPLC test solution.

Benzopyrene analysis was conducted by Chen et al. (2012) was used by connecting a C18 column (250 mm x 4.6 mm x 5 μm) to HPLC (Dionex Ultimate 3000, Thermo Scientific, San Jose, CA, USA), and water / ACN (9 : 1) A mixed solution was used, and the injection volume was 10 μl. The flow rate of the mobile phase was 1.5 ml / min, the column temperature was maintained at 25 ° C, and the detector was detected at an excitation wavelength of 361 nm and an emission wavelength of 405 nm using a fluorescence detector (FLD).

3) Degradation power of benzopyrene by plasma type

A) Degradation ability of benzopyrene by LPDP production conditions

The LPDP device employs a low-frequency glow method and generates low-temperature plasma in a manner that reduces the amount of heat generated by reducing the density of the gas as a medium for generating plasma through decompression. LPDP differs in production gas and generates plasmas of various characteristics according to the applied power and absolute pressure (vacuum degree).

The benzopyrene decomposition according to the treatment time of LPDP with different generation gas at an absolute pressure of 1.0 Torr and plasma output of 168W showed effective decomposition as shown in FIG. 8, and the best decomposition of air LPDP was found for each gas type, followed by oxygen and nitrogen. The resolution was reduced. Residual benzopyrene after LPDP treatment for 30 minutes decreased to 17.5, 17.9, and 18.5%, respectively, compared to pre-treatment for air-, oxygen-, and nitrogen-LPDP.

As a result of analyzing the benzopyrene decomposition pattern by LPDP by applying the model as shown in [Table 1], tailing was observed as shown in FIG. 9, and the log linear tail model or the Weibull tail model was used rather than the log linear model and the Weibull model. It turned out to be a better match. Table 2 shows the results of comparing the fit of each model.

Benzopyrene decomposition model formula Model name Model equation Log linear
(Bigrlow and Esty, 1920)

Log linear tail
(Greeraerd et al., 2000)

Weibull (Mafart et al., 2002)

Weibull tail
(Albert and Mafart, 2005)

C = remaining concentration (%)

C ₀ = initial concentration (%)

C _res = residual concentration (%)

k _max = first order decrease rate constant (1 / min)

m = curve shape factor

δ = initial decimal reduction time (min)

Comparison of fit of benzopyrene decomposition pattern model by air-LPDP Model SSE RMSE r ² Log linear 0.0113 ± 0.0128 0.1070 ± 0.0446 0.8359 ± 0.0441 Log linear tail 0.0065 ± 0.0038 0.0604 ± 0.0554 0.9598 ± 0.0278 Weibull 0.0027 ± 0.0017 0.0391 ± 0.0352 0.9778 ± 0.0222 Weibull tail 0.0023 ± 0.0012 0.0325 ± 0.0275 0.9902 ± 0.0110

Of the four models applied, tails reflecting models have small residual sum of squares (SSE) and mean square root error (RMSE), which lowers the rate of late decomposition compared to the initial decomposition rate and reflects benzopyrene remaining without decomposition. The SSE and RMSE of the Weibull tail model were slightly smaller than the log linear tail model, and the high crystallinity coefficient of 0.9902 was shown. The initial decimal reduction time (δ-value) and residual amount obtained by applying the Weibull tail model are shown in [Table 3]. Air-LPDP showed the smallest δ-value of 49.17 min, which showed the highest resolution, followed by oxygen-LPDP of 51.63 min and nitrogen-LPDP of 53.61 min, which had the lowest resolution. The residual ratio (C _res ) calculated by the Weibull tail model also showed the lowest value of air-LPDP at 15.13%, and oxygen-LPDP at 15.84% and nitrogen-LPDP at 16.21%, resulting in a final decomposition rate of 84.87%, respectively. , 84.16% and 83.79%. On the other hand, the shape factor of the decomposition curve (shape factor, m) showed a value of around 0.45 regardless of the generation gas, and it was found that it has a concave curve shape with a gentle slope depending on the processing time.

LPDP benzopyrene initial decimal reduction time (δ-value) by product gas, residual amount and curve shape factor  LPDP generating gas δ-value (min) C _res (%) m Air 49.17 ± 5.23 15.13 0.48 Nitrogen 53.61 ± 2.67 16.21 0.44 Oxygen 51.63 ± 2.59 15.84 0.42

As a result of measuring the benzopyrene reduction effect by adjusting the LPDP generating pressure to 0.1-5.0 Torr in order to investigate the effect of the degree of vacuum on the benzopyrene decomposition ability of LPDP, as shown in FIG. Torr showed the best resolution. However, when the vacuum degree was lowered to 0.5 Torr or less, the temperature of the treatment chamber rose to 65-86 ° C, and it was impossible to see it as a non-heating treatment. Within the range available for non-heating treatment, δ-value was calculated to be the lowest value of 49.17 min, as shown in [Table 4], at the absolute pressure of 1.0 Torr. It was 8.20 min. The residual rate was also 15.13% at 1.0 Torr and 0.63% at 0.1 Torr. From the above results, the optimum LPDP-producing gas was found to be air, and the optimum generating pressure was confirmed to be 1.0 Torr by non-heating treatment.

For example, in one embodiment of the present invention, when treated with LPDP, the plasma generation pressure may be 0.1 to 5.0 Torr, preferably 1.0 to 5.0 Torr for non-heating treatment. At this time, one of air, oxygen, and nitrogen may be used as the plasma generating gas.

Benzopyrene decomposition by LPDP generation pressure δ-value and Weibull tail model variable values Pressure (Torr) δ-value (min) C _res (%) m 0.1 8.20 ± 2.65 0.63 0.43 0.5 17.55 ± 2.01 5.24 0.31 1.0 49.17 ± 5.23 15.13 0.48 3.0 82.40 ± 10.60 21.37 0.44 5.0 255.12 ± 89.42 35.48 0.74

On the other hand, when looking at the effect of power consumption on the benzopyrene decomposition power, as shown in Fig. 11 and [Table 5], the benzopyrene decomposition power at 168 W with the maximum power of the LPDP generator is 144 W or less. It was found that the required critical power exists. The calculated residual ratio and δ-value were also 15.13% and 49.17 min, respectively, where the power 168 W was the lowest, respectively, and showed a significantly smaller value compared to the case where the power was 144 W or less, showing a great difference in resolution.

Benzopyrene decomposition of air-LPDP by power consumption δ-value and Weibull tail model variable values Power (W) δ-value (min) C _res (%) m 168 49.17 ± 5.23 15.13 0.48 144 60.39 ± 6.84 17.78 0.58 95 69.82 ± 10.75 19.05 0.47 74 108.39 ± 12.53 25.71 0.51

B) Decomposition of benzopyrene by DBDP treatment conditions

The dielectric barrier discharge plasma (DBDP) is a method in which a plasma is generated from an electrode to a floor by wrapping an electrode with a ceramic dielectric, converting an AC 220V voltage to a DC 20kV high voltage, and applying a pulsed square wave. At this time, the sample was processed by adjusting the current strength and the distance between the electrode and the treatment plate (electrode gap, EG).

When DBDP was treated by adjusting the electrode spacing (the gap between the electrode and the treatment plate) and the current intensity to 2.0-3.0 mm and 2.0-3.0 A, respectively, as shown in Figs. 12A-F, benzopyrene decomposed very effectively. Also, as the current intensity increased, the resolution of DBDP increased. In particular, when the electrode spacing was narrowed to 2.0 mm and the current intensity was treated with 3.0 A for 30 minutes or more, benzopyrene was shown to decompose more than 90%, confirming a very good decomposition ability.

The benzopyrene decomposition pattern by DBDP was also best analyzed by the Weibull tail model as shown in Fig. 13 and [Table 6], and the variable values of the Weibull tail model for each treatment condition are shown in [Table 7].

Comparison of benzopyrene decomposition pattern model by DBDP Model SSE RMSE r ² Log linear 0.0197 ± 0.0062 0.1389 ± 0.0218 0.7610 ± 0.0431 Log linear tail 0.0074 ± 0.0032 0.0842 ± 0.0194 0.9229 ± 0.0235 Weibull 0.0010 ± 0.0006 0.0307 ± 0.0084 0.9872 ± 0.0085 Weibull tail 0.0009 ± 0.0004 0.0268 ± 0.0075 0.9896 ± 0.0063

DBDP showed a lower δ-value and a residual rate as the electrode gap was narrowed and the current strength was higher, indicating that the benzopyrene decomposition power was high. Became. The minimum δ-value of DBDP was 5.91 min at an electrode spacing of 2.0 mm and a current strength of 3.0 A.

DBDP benzopyrene decomposition δ-value and Weibull tail model variable values by electrode spacing and current intensity Electrode gap (mm) Current (A) δ-value (min) C _res (%) m 2.0 2.0 8.61 ± 1.88 6.01 0.44 2.5 7.17 ± 1.04 4.78 0.33 3.0 5.91 ± 0.83 4.06 0.31 2.5 2.0 9.67 ± 1.41 7.12 0.43 2.5 6.44 ± 1.03 6.15 0.31 3.0 7.45 ± 0.73 5.74 0.38 3.0 2.0 12.59 ± 4.01 8.11 0.59 2.5 10.71 ± 4.48 7.39 0.34 3.0 7.57 ± 1.29 6.35 0.38

Despite the excellent decomposition effect of benzopyrene, DBDP has a narrow electrode spacing, as shown in [Table 7], which limits the size and shape of the material to be treated. In addition, due to the possibility of arcing due to accidental contact between the treatment sample and the electrode, the electrode gap must be widened for field application of the DBDP, and improvement for large-scale equipment production and operation that generates plasma at a wide electrode gap is required.

For example, an ADDAP device, which is an indirect plasma processing method described below, may be implemented for DBDP field application, which will be described later.

C) Decomposition of benzopyrene by CDPJ treatment conditions

The Corona Discharge Plasma Jet (CDPJ) processing device is a device that installs two stainless steel ring-shaped electrodes in a ceramic shield to generate plasma and jets the generated plasma in a jet form using a blower (blower). At this time, the sample was processed by adjusting the current intensity and the distance (discharge distance, span length, SL) between the plasma discharge port and the sample processing plate.

When CDPJ treatment was performed for 30 minutes by adjusting the current intensity and discharge distance, benzopyrene was decomposed as in FIGS. 14A-C. During the CDPJ treatment, the shorter the discharge distance, the higher the current intensity, the higher the resolution of the CDPJ, but the lower the resolution compared to the previous DBDP.

The benzopyrene decomposition pattern by CDPJ was also best analyzed by the Weibull tail model, and the variable values of the Weibull tail model by treatment conditions are shown in [Table 8]. The δ-value of CDPJ showed a relatively large value compared to the previous LPDP or DBDP, so it was confirmed that the resolution was low. The lowest δ-value and residual ratio calculated by the Weibull tail model were calculated to be 37.34 min and 10.47% at a discharge distance of 15 mm and a current intensity of 1.5 A, respectively.

Despite the relatively low benzopyrene decomposition ability, CDPJ has the advantage that it can be used regardless of the size and shape of the sample, so it can be said to be a plasma method suitable for practical application in the field. For example, in one example, in the case of the CDPJ method, the current intensity may be set to 1.0 to 1.5A. In addition, in the CDPJ method, the discharge distance SL may be 15 to 30 mm.

In order to apply CDPJ in the field, power enhancement and scale-up are required to increase resolution. For the ICDPJ method, which is an improvement of the CDPJ method, refer to the following description.

CDPJ benzopyrene decomposition δ-value and Weibull tail model variable values by current intensity and discharge distance Span length (mm) Current (A) δ-value (min) C _res (%) m 15 1.00 44.51 ± 4.18 12.31 0.42 1.25 41.22 ± 4.00 11.74 0.37 1.50 37.34 ± 5.27 10.47 0.39 25 1.00 75.78 ± 12.56 19.05 0.38 1.25 53.40 ± 7.84 15.49 0.30 1.50 51.69 ± 9.05 13.48 0.43 30 1.00 163.13 ± 19.72 20.41 0.32 1.25 155.01 ± 26.05 19.05 0.27 1.50 76.30 ± 8.74 16.59 0.30

D) Decomposition of benzopyrene by ICDPJ treatment conditions

ICDPJ is a field-applied low-temperature plasma processing system that can process plasma without restrictions according to the size and shape of the food to be processed. By improving the existing CDPJ processing device, it strengthens the current strength and intermittently repeats plasma processing and cooling for a short time. A method of controlling the temperature was employed.

Intermittent CDPJ (Intermittent CDPJ) of intermittent CDPJ method that repeats treatment and cooling for a certain period of time as a method to maintain non-thermal treatment conditions by increasing the power and processing capacity and lowering the heating effect for field application based on the possibility of benzopyrene decomposition by CDPJ ) And tested. As a result of investigating ICDPJ's benzopyrene decomposition ability, it showed relatively low decomposition ability similar to the previous CDPJ as shown in FIGS. The δ-value and model variable values for each treatment condition are shown in [Table 9], and the minimum δ-value and residual ratio were 49.77min and 15.13%, respectively, when the discharge distance was 30 mm and treated with current 3.0 A. .

In one example, the current intensity of the ICDPJ method may be in the range of 2.0 to 3.0 A. In addition, the discharge distance SL in the ICDPJ method may be 30 to 50 mm.

ICDPJ benzopyrene decomposition δ-value and Weibull tail model variable values according to discharge distance and current intensity Span length (mm) Current (A) δ-value (min) C _res (%) m 30 2.0 64.92 ± 6.79 17.38 0.37 2.5 58.25 ± 5.70 15.85 0.35 3.0 49.77 ± 5.60 15.13 0.42 40 2.0 98.94 ± 11.02 21.38 0.43 2.5 90.23 ± 23.78 20.42 0.38 3.0 82.29 ± 8.03 19.49 0.33 50 2.0 159.89 ± 12.68 27.54 0.35 2.5 129.42 ± 7.19 22.38 0.34 3.0 110.59 ± 16.95 20.89 0.36

3) Reduction of benzopyrene content by plasma and benzopyrene by plasma

A) Benzopyrene content of sesame seeds by roasting conditions

The benzopyrene content of the roasted sesame seeds according to different roasting conditions using a commercial roasting device is shown in [Table 10]. The content of benzopyrene was high according to the roasting temperature, and 8.51 ppb of sesame seeds roasted under the conditions applied when sesame oil was milked (final temperature of 220 ° C) and 9.87 ppb of perilla roasted under normal roasting conditions (final temperature of 210 ° C). Were analyzed. On the other hand, the excessively roasted sesame seeds (final temperature of roasting 245 ° C) had a benzopyrene content of 51.52 ppb, and perilla (final temperature of roasting 230 ° C) showed 77.24 ppb.

Benzopyrene content of sesame seeds by roasting conditions Sample code Benzopyrene (ppb) RRS 8.51 ORS 51.52 RRP 9.87 ORP 77.24

As a result of analyzing the benzopyrene content of sesame oil and perilla oil obtained by using roasted sesame oil, 3.27 ppb of sesame oil that is normally roasted and oiled is 21.44 ppb of sesame oil that is obtained by frying excessively, and 4.43 ppb of oil that is typically roasted and oiled. However, the perilla oil obtained by frying excessively increased the benzopyrene content after oiling compared to the roasted sesame seeds before 31.24 ppb, which is higher in the heated state in the oil press, so that the benzopyrene content of the milk is higher than the roasted sesame oil. Is estimated. On the other hand, in the case of excessively roasted sesame seeds, the content of benzopyrene after oil was reduced, which is thought to be because some of the benzopyrenes in the roasted sesame seeds were removed from the oilseed rape.

Benzopyrene content of oil by roasting conditions Sample code Benzopyrene (ppb) RRS-oil (RRSO) 3.27 ORS-oil (ORSO) 21.44 RRP-oil (RRPO) 4.43 ORP-oil (ORPO) 31.24

B) Reduction of benzopyrene in sesame and oil by plasma treatment

When the roasted sesame seeds were treated with plasma using different commercial roasters, the benzopyrene content according to the treatment time was decreased by LPDP, CDPJ, and ICDPJ treatment as shown in FIGS. 16A-D. Plasma roasted sesame seeds under normal conditions with benzopyrene content of 0.41-0.55 ppb was reduced by 41.31-52.61% by 30 min treatment, and when overly roasted sesame seeds with a benzopyrene content of 2.41-7.17 ppb were treated for 30 minutes 32.96-49.41 % Reduction effect. From the above results, it was confirmed that the benzopyrene of the roasted sesame seeds was lowered by the plasma treatment. The effect of plasma reduction on benzopyrene was superior to that of stir-fried sesame seeds under normal roasting conditions.

It was confirmed that the content of benzopyrene in the oil milked using a vegetable oil machine after plasma treatment of roasted sesame under different conditions using a commercial roaster also decreases the content of benzopyrene in oil according to the plasma treatment time as shown in FIG. 17A-D. Became. Normally, the benzopyrene content of oil milked from roasted sesame was 0.55-0.80 ppb, and the content of benzopyrene in milking oil was also lowered depending on the plasma treatment time of roasted sesame seeds. When milking from the plasma-treated sesame seeds for 30 minutes, 35.41-49.18 %. Reduction effect was confirmed even in oil milked from excessively roasted sesame seeds with a benzopyrene content of 2.03-5.40 ppb, and plasma treatment for 30 minutes showed a reduction effect of 35.09-47.41%.

From the above results, it was confirmed that the plasma treatment was effective in reducing benzopyrene of roasted sesame seeds, and did not show a significant difference by plasma type, and the benzopyrene reduction effect was maintained even in milked oil.

[Example-Roasted Coffee]

18A and 18B are CDPJ processing of roasted beans (City Roasting to Full City Roasting) and super roasted (French Roasting to Italian Roasting), respectively. After benzopyrene content is shown. At this time, the CDPJ treatment conditions were an electrode sample interval of 15 mm and a current strength of 1.50 A. As a result of confirming the benzopyrene content of the roasted coffee beans treated with CDPJ, as shown in FIGS. 18A and 18B, as the treatment time increased, the residual benzopyrene content of the coffee decreased. As a result of treatment for 60 minutes, the benzopyrene content decreased from the initial value of 1.062 μg / kg to 0.512 μg / kg after 60 minutes treatment compared to before treatment, and 48.15% remained and decreased by 51.85%, and the initial value for superpower distribution was 1.879 After 60 min treatment at μg / kg, it decreased to 1.192 μg / kg, leaving 63.44% remaining and 36.56% decreasing.

In addition, FIGS. 19A and 19B are ICDPJs for roasting beans (City Roasting to Full City Roasting) and super roasting (French Roasting to Italian Roasting), respectively. It shows the result of checking the benzopyrene content after treatment. At this time, ICDPJ treatment conditions were electrode sample intervals of 25 mm and current strength of 3.0 A. As a result of confirming the benzopyrene content after treating ICDPJ on the roasted coffee beans, the residual benzopyrene content of the coffee decreased as the treatment time increased as shown in FIGS. 19A and 19B. When treated for up to 60 minutes, the benzopyrene content decreased from the initial value of 1.062 μg / kg to 0.545 μg / kg after 60 minutes of treatment, compared with before treatment, and 51.33% remained and 48.67% decreased. After processing for 60 minutes at a value of 1.879 μg / kg, it decreased to 1.209 μg / kg, resulting in 64.34% remaining and 35.66% decreasing.

[Example-Roasted barley for barley tea]

The change in benzopyrene content of roasted barley by CDPJ treatment is shown in FIG. 20, and the content of residual benzopyrene decreased as the treatment time increased, and by 120 minutes treatment, the content of benzopyrene decreased by 66.86% from 0.0095 ug / kg to 0.0032 ug / kg. Did.

The change in benzopyrene content of roasted barley by ICDPJ treatment was reduced by 52.96% by 120-minute treatment as shown in FIG. 21, thereby reducing benzopyrene of grilled barley from 0.0095 ug / kg to 0.0047 ug / kg.

[Example-Edible oil and fat]

The edible oil such as sesame oil and perilla oil is put in a container, and plasma gas is supplied through a porous dispersion pipe (sprger) installed at the bottom of the container to increase the edible oil layer in a bubble state for a period of time to exert its effect. Treatment.

25A and 25B show the results of confirming the benzopyrene content after ADDAP treatment of sesame oil and perilla oil, respectively.

In order to confirm the effect of reducing benzopyrene in sesame oil by the treatment of plasma gas, the indirect plasma was treated with sesame oil roasted at a high temperature (245 ° C), and then the benzopyrene content according to the treatment time was checked. Referring to Figure 25a, the benzopyrene content of sesame oil without any treatment was confirmed to be 2.103 μg / kg, and in sesame oil treated with plasma, it was 1.623 μg / kg at 30 minutes and 1.387 μg / kg at 60 minutes. , 1.187 μg / kg at 90 minutes, 0.833 μg / kg at 120 minutes, and the benzopyrene content decreased as the treatment time increased and 61.59% decreased at 120 minutes, showing the greatest change. In order to confirm the change of benzopyrene content by air inflow as well as the effect of plasma, the result of treating sesame oil by injecting untreated plasma is 2.021-2.084 μg / kg, which is almost no difference from the initial value. Reduction of benzopyrene by was confirmed.

In order to confirm the effect of reducing benzopyrene in perilla oil by the treatment of plasma gas, plasma was treated with perilla oil roasted at high temperature (230 ° C), and then the benzopyrene content according to the plasma treatment was confirmed. Referring to Figure 25b, the benzopyrene content of untreated perilla oil was confirmed to be 1.888 μg / kg, and in the perilla oil treated with plasma, the benzopyrene content decreased as the treatment time increased, and treated for 30 minutes, 60 minutes, 90 minutes, and 120 minutes, respectively. At 1.551 μg / kg, 1.255 μg / kg, 1.081 μg / kg, and 0.777 μg / kg, 58.84% was reduced from 120 minutes, which had the longest treatment time, confirming the effect of reducing benzopyrene in perilla oil by injection of plasma-treated air. Could. The benzopyrene content of perilla oil treated by injecting only plasma-free air was 1.816-1.889 μg / kg, and there was little difference in perilla oil and benzopyrene content, so it was confirmed that plasma-treated air had no benzopyrene reduction effect. .

[System for reducing harmful products including benzopyrene in agricultural products processed using low-temperature plasma]

Next, a system for reducing harmful products including benzopyrene of agricultural products processed using low temperature plasma according to one aspect of the present invention will be described with reference to the drawings. At this time, embodiments of the method for reducing harmful products including benzopyrene of agricultural products processed using low temperature plasma according to the example of the above-described invention and FIGS. 1 to 5 may be referred to, and redundant descriptions may be omitted. 8 to 25B may also be referred to.

6 is a block diagram schematically showing a system for reducing harmful products, including benzopyrene, in agricultural products processed using low temperature plasma according to another embodiment of the present invention, and FIGS. 7A-C are each an embodiment of the present invention. It is a block diagram schematically showing a system for reducing harmful products including benzopyrene in agricultural products processed using low-temperature plasma.

Referring to FIGS. 6 to 7C, a system for reducing harmful products including benzopyrene generated by high temperature processing of agricultural products is a system for reducing harmful products including benzopyrene in agricultural products processed using a low temperature plasma according to one example. . In one example, a system for reducing harmful products including benzopyrene of agricultural products using low-temperature plasma includes a plasma generator 300, a blower 400, a processing chamber 100, and a control unit 500. For example, a processed agricultural product may be an intermediate product in a processed state or a final product subject to a transaction after processing is completed.

The plasma generator 300 generates a low temperature plasma with respect to the plasma generating gas. For example, the plasma generator 300 may be installed in the processing chamber 100. For example, the plasma generator 300 generates a low temperature plasma with respect to, for example, plasma generating gas, which is any one of air, oxygen, and nitrogen.

The blower 400 supplies plasma-generated plasma gas from the plasma generator 300 to the processing chamber 100. For example, the blower 400 may supply the plasma gas generated by the plasma generator 300 to the processing chamber 100 by blowing. In addition, referring to FIG. 7C, in one example, the blower 400 may supply plasma gas generated by the plasma generator 300 to the porous dispersion pipe of the oil and fat container in the processing chamber 100.

The processing chamber 100 accommodates the processed product that has undergone high-temperature processing for agricultural products, and performs low-temperature plasma indirect processing using plasma gas supplied by the blower 400 to reduce harmful products including benzopyrene.

Harmful products including benzopyrene, which is a reduction target, are produced by high-temperature processing treatment for agricultural products belonging to one of seed, grain, nut, and bean.

For example, the processing result, which is the object of the low temperature plasma treatment, may be a direct result of a high temperature processing treatment, such as a roasting treatment, or a further processing result to which a processing treatment for a direct result is added.

The control unit 500 controls the generation of low temperature plasma through the plasma generator 300, the blowing through the blower 400, and the low temperature plasma indirect processing of the processing result in the processing chamber 100. For example, in the case of generating plasma by the LPDP method, the control unit 500 may adjust the pressure. When generating plasma in the DBDP method, the control unit 500 may adjust the current intensity and / or the electrode spacing. When generating plasma in the CDPJ method, the control unit 500 may adjust the current intensity and / or discharge distance. When generating plasma in the ICDPJ method, the control unit 500 may adjust the current intensity and / or discharge distance.

Referring to Figures 7a to 7b, in one example, the hazardous product reduction system may further include a transfer unit (200). The transfer unit 200 may transfer the processing result to the processing chamber 100 and discharge the processing result of the low temperature plasma indirect processing. For example, the transfer unit 200 may transfer the direct processing result of the frying process by the high temperature processing processing unit 600 to the additional processing result to which the processing processing is added to the direct result as a processing processing result.

In addition, the processing chamber 100 may include a passage portion 100a for transport and discharge by the transfer unit 200 and an exhaust port 100b for discharging gas in the chamber.

In addition, referring to FIG. 7B, in another example, the system for reducing harmful substances may further include a high temperature processing unit 600. The high temperature processing unit 600 stirs agricultural products. The result of the roasting treatment is transferred to the processing chamber 100 by the transfer unit 200, or after further physical processing, such as powdering or milking by pressing, further processing results are transferred by the transfer unit 200 It may be transferred to the processing chamber 100.

For example, the high-temperature processing unit 600 may perform high-temperature processing, for example, roasting, of agricultural products belonging to any one of seeds, grains, nuts, and beans. In one example, the high-temperature processing unit 600 is any one of seeds, nuts, and beans in the whole stage of milking of oils and fats, grains, or direct foods or foods in the raw material manufacturing process of coffee or tea beverages. In the process of processing into ingredients, the agricultural products belonging to any one of seeds, grains, nuts, and beans can be roasted.

Referring to FIG. 7C, in one example, the processing chamber 100 accommodates the oil and fat container 110 containing the oil and fat, and the porous dispersion pipe 111 may be provided at the bottom of the oil and fat container 110. At this time, the plasma gas supplied by the blower 400 is discharged in a bubble state into the oil and fat through the porous dispersion pipe 111, and low temperature plasma indirect treatment may be performed.

In addition, in one example, the plasma generator 300 may be formed by any one of reduced pressure discharge plasma (LPDP), dielectric barrier discharge plasma (DBDP), corona discharge plasma jet (CDPJ), and intermittent corona discharge plasma jet (ICDPJ). Low-temperature plasma can be generated in this manner.

At this time, in one example, the plasma generator 300 generates a low temperature plasma in a manner by a dielectric barrier discharge plasma (DBDP), and uses any one of air, oxygen, and nitrogen as the plasma generating gas.

For example, in one example, the plasma generator 300 may generate plasma in a current intensity range of 0.4 to 0.6 A by using air as a plasma generating gas.

[Example-ADDAP system]

A) ADDAP system structure and characteristics

In order to process a large amount of grain foods such as grains and sesame seeds, a system was developed that exhibits an indirect treatment effect by continuously introducing plasma-treated air. For example, plasma is generated using a DBDP method that has excellent decomposition effects of harmful substances, and air that is plasma-treated is introduced into the treatment chamber by using air blowers to process food in large quantities regardless of size and shape. A food processing system was fabricated and named after-glow dielectric discharge air plasma (ADPAP) system. For example, plasma generation in the ADDAP system may be generated in other ways as well as the DBDP method. For example, the ADDAP system may be implemented based on the block diagrams shown in FIGS. 6 to 7C.

As a result of generating the plasma by adjusting the current intensity in the ADDAP system, plasma began to be generated from 0.15 A and stable plasma generation was maintained above 0.40 A. As the current intensity increased, plasma of bright light was generated, but the plasma generation became unstable above 0.70 A, and arcing began to occur at 0.80 A.

The result of measuring the power consumption by current intensity in ADDAP increased with the current intensity, and the power consumption in the range of 0.40-0.60 A generating stable plasma was found to be 169.51-201.77 W.

As a result of measuring the temperature of the ADDAP processing chamber at a current intensity of 0.40-0.60 A, the temperature rise was found to be insignificant since it was about 23.4 ° C even after 180 min treatment.

On the other hand, when looking at the gas composition of the ADDAP processing chamber according to the current intensity, the NO and NO ₂ concentrations were generated in proportion to the current, while the CO concentration began to be generated at 0.6 A or more.

B) ADDAP's benzopyrene reduction effect

As a result of treating benzopyrene by varying the current strength of ADDAP, as shown in FIG. 22, as the treatment time increased, the residual strength of benzopyrene decreased as the current strength increased, and the decomposition power increased. It was reduced to 54.41%. As a result of analysis using Weibull tail model, it showed δ-value 89.07 min and residual rate 38.87% as shown in [Table 12].

ADDAP benzopyrene decomposition δ-value and other variable values by current intensity Current (A) δ-value (min) C _res (%) M 0.4 1689.47 ± 262.12 67.66 0.39 0.5 284.44 ± 15.05 42.65 0.50 0.6 89.07 ± 25.47 38.87 0.59

C) Reduction of benzopyrene in food by ADDAP treatment

As a result of analyzing the benzopyrene content after processing the roasted sesame in the ADDAP system, it was confirmed that benzopyrene is reduced according to the treatment time as shown in FIGS. 23A-D. After 240 minutes of treatment time, the benzopyrene content was lowered to 45.35-59.94% compared to before treatment.

After the ADDAP treatment of the roasted sesame seeds, the benzopyrene content of the sample milked with the oil was measured and decomposed according to the plasma treatment time as shown in FIGS. 24A-D, and after processing for 240 minutes, up to 55.08-59.69% of the content before treatment Decreased.

Referring to FIG. 7C, after analyzing sesame oil and perilla oil in the ADDAP system, the result of analyzing the benzopyrene content was confirmed to decrease benzopyrene according to the treatment time as shown in FIGS. 25A-B. See the above for specific results.

On the other hand, when the sesame oil and perilla oil are treated in the ADDAP system, the changes in acid value and peroxide value are shown in [Table 13] below.

Changes in acid value and peroxide value of sesame oil and perilla oil by ADDAP treatment Plasma treated time (min) Acid value (mg / g) POV (meq / kg) Sesame seed oil Perilla seed oil Sesame seed oil Perilla seed oil 0 0.58 ± 0.10 a 1.84 ± 0.13 a 2.30 ± 0.28 a 33.95 ± 2.19 a 30 0.57 ± 0.06 a 1.82 ± 0.22 a 2.40 ± 0.14 a 35.30 ± 4.38 a 60 0.60 ± 0.07 a 1.83 ± 0.11 a 2.40 ± 0.14 a 33.40 ± 7.07 a 90 0.57 ± 0.13 a 1.81 ± 0.12 a 2.45 ± 0.21 a 35.35 ± 3.04 a 120 0.57 ± 0.05 a 1.89 ± 0.16 a 2.35 ± 0.21 a 34.60 ± 1.54 a

Values are given as mean ± SD (n = 3). Distinct letter within the same column indicate significant differences (p <0.05).

Referring to [Table 13], the results of measuring the acid value and peroxide value (POV) of sesame oil and perilla oil according to ADDAP treatment time are shown to confirm the quality change of sesame oil and perilla oil by ADDAP treatment. have. The initial acid value of sesame oil was 0.57-0.60 mg / g, and perilla oil was 0.90-0.94 mg / g. The acid values of sesame oil and perilla oil by ADDAP treatment were 0.57-0.60 mg / g and 1.81-1.89 mg / g, respectively, and no change according to ADDAP treatment was confirmed. The peroxide value before treatment was 2.30 meq / kg for sesame oil, 33.95 meq / kg perilla oil, and sesame oil after treatment was 2.35-2.45 meq / kg, perilla oil was 33.40-35.35 meq / kg, so there was no significant difference according to treatment time. It was confirmed that there was no change in the peroxide value by the treatment of ADDAP until 120 minutes.

In the above, the above-described embodiments and the accompanying drawings are not intended to limit the scope of the present invention, but have been exemplarily described to help a person of ordinary skill in the art to understand the present invention. In addition, embodiments according to various combinations of the above-described configurations can be obviously implemented to those skilled in the art from the foregoing detailed description. Accordingly, various embodiments of the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention, and the scope of the present invention should be interpreted according to the invention described in the claims, and is usually in the art. It includes various changes, alternatives, and equivalents by those with knowledge of

100: processing chamber, 100a: passage
100b: exhaust port, 110: oil container
111: porous dispersion pipe 200: transfer unit
300: plasma generator, 400: blower
500: control unit, 600: high temperature processing unit

Claims (15)

In the method for reducing harmful products, including benzopyrene produced by high-temperature processing of agricultural products,
Comprising a step of reducing the harmful products to reduce the harmful products by performing a low-temperature plasma treatment on the processing result of the high-temperature processing process for the agricultural products,
Method for reducing harmful products including benzopyrene in agricultural products processed using low temperature plasma.
In claim 1,
Before the step of reducing the harmful products, further comprising a high-temperature processing step of performing the high-temperature processing to cause the generation of the harmful products including the benzopyrene for the agricultural products of low-temperature plasma of agricultural products processed products using low-temperature plasma Method for reducing harmful products including benzopyrene.
In claim 2,
The agricultural products belong to one of species, grains, nuts, and beans,
The result of the processing treatment is a direct result of the high temperature processing treatment or a further processing result to which the processing treatment for the direct result is added,
Method for reducing harmful products including benzopyrene of agricultural products processed using low-temperature plasma, characterized in that the high-temperature processing step of performing the roasting treatment for the agricultural products.
In claim 3,
The roasting process is performed in the process of preparing raw materials for coffee or tea beverages from the agricultural products belonging to any one of the seeds, cereals, or in the preceding stage of milking the oil from the agricultural products belonging to any one of the seeds, nuts, and soybeans, or the Method for reducing harmful products, including benzopyrene, of agricultural products processed using low-temperature plasma, characterized in that it is performed in the process of processing directly from the agricultural products belonging to any one of seeds, grains, nuts, and beans to food or food materials.
In claim 4,
In the raw material manufacturing process of the coffee or tea beverage, the roasting process is a roasting of coffee beans or roasting for any one of wheat, barley, and corn, and a method for reducing harmful products including benzopyrene in agricultural products processed using low-temperature plasma. .
In claim 4,
The roasting process as a pre-step of milking the fats and oils is frying for any one of sesame, perilla, rapeseed, and sunflower seeds,
The low-temperature plasma treatment method for reducing harmful products, including benzopyrene, in agricultural products processed products using low-temperature plasma, characterized in that is performed on the stir-processed product or milk fat.
In any one of claims 1 to 6,
The low-temperature plasma treatment is a low-temperature plasma treatment by any one of reduced-pressure discharge plasma (LPDP), dielectric barrier discharge plasma (DBDP), corona discharge plasma jet (CDPJ), and intermittent corona discharge plasma jet (ICDPJ). Method for reducing harmful products including benzopyrene in agricultural products using plasma.
In claim 7,
Method for reducing harmful products including benzopyrene of agricultural products processed using low temperature plasma, characterized in that any one of air, oxygen, and nitrogen is used as a plasma generating gas in the step of reducing harmful products.
In the system for reducing harmful products, including benzopyrene produced by high-temperature processing of agricultural products,
A plasma generator that generates a low temperature plasma with respect to the plasma generating gas;
A blower that supplies plasma-generated plasma gas by the plasma generator;
A processing chamber for receiving the processing result of the high-temperature processing treatment for the agricultural product, but performing low-temperature plasma indirect treatment using the plasma gas supplied by the blower to reduce the harmful product including benzopyrene; And
Including the control unit for controlling the low-temperature plasma indirect processing of the low-temperature plasma through the plasma generator, the blow-through through the blower and the processing result in the processing chamber, including benzopyrene of agricultural products processed using low-temperature plasma Hazardous product reduction system.
In claim 9,
The agricultural products belong to one of species, grains, nuts, and beans,
The system for reducing harmful products further includes a transfer unit that transports the processing result into the processing chamber and discharges the processing result resulting from the low temperature plasma indirect treatment,
The treatment chamber is a system for reducing harmful substances including benzopyrene of agricultural products processed using low-temperature plasma, characterized in that it comprises a passage for transport and discharge by the transfer unit and an exhaust port for discharging gas in the chamber.
In claim 10,
The harmful product reduction system further includes a high-temperature processing unit for roasting the agricultural products,
The transfer unit transfers the direct result to which the high-temperature processing unit is stir-processed or the additional processing result to which the processing is added to the direct result as the processing result,
The high temperature processing unit is any one of the seeds, nuts, and beans in the whole stage of milking of the oil and fat, any of the seeds, grains, or directly processed into food or food materials in the raw material manufacturing process of coffee or tea beverages. A system for reducing harmful products, including benzopyrene, in agricultural products processed using low-temperature plasma, characterized in that the agricultural products belonging to any one of the seeds, grains, nuts, and beans are roasted.
In claim 9,
The result of the processing treatment is oils and fats milked through the high-temperature processing treatment for the agricultural products belonging to one of the seeds, nuts, and soybeans,
The processing chamber accommodates the oil and fat container containing the oil and fat,
A porous dispersion pipe is provided at the bottom of the oil and fat container,
Hazardous products including benzopyrene of agricultural products processed using low-temperature plasma, characterized in that the plasma gas supplied by the blower is discharged into the state of the bubbles into the oil and fat through the porous dispersion pipe and performing the low-temperature plasma indirect treatment. Reduction system.
In any one of claims 9 to 12,
The plasma generator generates the low-temperature plasma by a method of any one of depressurized discharge plasma (LPDP), dielectric barrier discharge plasma (DBDP), corona discharge plasma jet (CDPJ), and intermittent corona discharge plasma jet (ICDPJ). A system for reducing harmful products including benzopyrene in agricultural products processed using low temperature plasma.
In claim 13,
The plasma generator generates the low-temperature plasma in a manner by the dielectric barrier discharge plasma (DBDP),
A system for reducing harmful products including benzopyrene of agricultural products processed using low-temperature plasma, characterized in that any one of air, oxygen, and nitrogen is used as the plasma-generating gas.
In claim 14,
The plasma generator using the air as the plasma generating gas to produce a plasma in the current strength of 0.4 ~ 0.6 A range, a system for reducing harmful products, including benzopyrene of agricultural products processed using low-temperature plasma.

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