JP6546578B2 - Method for extracting fat-soluble components from algae - Google Patents

Description

  The present invention relates to a method of easily extracting fat-soluble components such as fucoxanthin, eicosapentaenoic acid (EPA), β-carotene and the like contained in algae at a high concentration, and food and cosmetic compositions containing the extract.

  Alga contains useful nutrients such as minerals, vitamins and dietary fibers (fucoidan etc.), and has recently been attracting attention as a health food.

The algae contains fat-soluble components such as β-carotene and EPA, and these fat-soluble components are useful as nutritional components or as materials for cosmetics.
Specifically, since β-carotene has a strong antioxidative effect, it can be expected to be effective in skin care and anti-aging when added to cosmetics and leads to prevention of arteriosclerosis and lifestyle-related diseases if it is taken as a food.
If beta-carotene is taken into the body, it is converted to vitamin A and used according to the required amount.

  Since EPA has the function of reducing neutral fat and cholesterol in the blood, taking it as a food can reduce the risk of cerebral thrombosis and cerebral infarction. As it has a strong antioxidant action like β-carotene, it can be expected to be effective in skin care and anti-aging when added to cosmetics.

  Among algae-derived fat-soluble components, fucoxanthin has recently attracted attention. Fucoxanthin is a kind of carotenoid (xanthophyll) contained in brown algae, and is a component contained in seaweeds such as wakame, mozuku, kelp and hijiki which are eaten on a daily basis. In recent years, fucoxanthin has been reported to have health functional effects such as antioxidative action, antitumor action, nerve cell protective action, and suppression of blood sugar level elevation, and is expected to be used in health food and cosmetics etc. .

On the other hand, as a method of extracting a lipid-soluble component of algae, a method of extracting using an organic solvent having compatibility with water, such as ethanol, as the extraction solvent is performed.
However, in general, even in cells producing lipid-soluble components such as carotenoids and EPA, the content of fat-soluble components contained in the cells is low. For example, fucoxanthin contained in seaweed generally used for food is , 0.2 mg / g or less per wet weight extremely small amount.
Therefore, in order to obtain a fat-soluble component in an amount that exhibits effective functionality, it is necessary to use an organic solvent compatible with water, such as ethanol, and then concentrate using various methods to solubilize it in fats and oils. There is a problem in that it requires a large amount of raw materials and takes time and effort.
For this reason, development of a method capable of extracting lipid-soluble components from algae efficiently and simply is required.
Furthermore, fucoxanthin is a substance that is easily decomposed with the passage of time. Therefore, there is a need for an extraction method that can stably store fucoxanthin for a long period of time.

Various methods have been developed to efficiently extract fat-soluble components from algae.
Among them, there is a method of physically disrupting cells and removing the fat-soluble component contained in the cells.
For example, Patent Document 1 describes a method of dissolving carbon dioxide in a dispersion containing microalga and applying shear force with a high-pressure dispersing device to break up the microalga and extract lipids. However, in the method described in Patent Document 1, water is used as a solvent contained in the dispersion, and it is necessary to subsequently perform solvent extraction, centrifugation, stationary treatment, and / or column chromatography, etc. in order to separate lipids. And it takes time and effort.

Patent Document 2 describes a method for producing a seaweed-derived product material containing a physiologically active substance consisting of a fat-soluble component, wherein the seaweed is refined and the refined seaweed is incubated in an aqueous enzyme solution containing a seaweed cell wall degrading enzyme Thereafter, a manufacturing method is described in which the liquid component is separated from the solid component and the solid component is dried to obtain a seaweed-derived product material.
In the method described in Patent Document 2, since the fat-soluble component is extracted in the aqueous enzyme solution, it is necessary to separate the fat-soluble component from the aqueous solution thereafter, and it takes time and effort to separate.

Patent Document 3 describes a method of extracting fat-soluble components into fats and oils, etc. by adding fats and oils such as olive oil, waxes or hydrocarbons to pieces or powder of seaweed, stirring and then grinding at normal temperature. It is done.
Although the example which used extracted fats and oils etc. for cosmetics as it is described in patent document 3, since it is necessary to perform sterilization operation after that in order to use as foodstuffs, processing after extraction requires time and effort. It has the problem of Furthermore, in the method described in Patent Document 3, since the fat-soluble component extracted in the fat and oil is not uniformly dispersed, it has a problem that it is difficult to add it at an accurate concentration when added to food and cosmetics thereafter.

JP 2015-124239 A Patent No. 5391536 Japanese Patent Application Publication No. 2004-089158

  The present invention provides a method of easily extracting fat-soluble components such as fucoxanthin, EPA, β-carotene and the like contained in algae at a high concentration, and a food and cosmetic composition containing the extract.

Invention, fucoxanthin from silicofluoride algae A method for extracting at a concentration of at least 1.5 mg / g, (1) a step of pulverizing the silicofluoride algae, chain medium to silicofluoride algae were ground (2) according to claim 1 And (3) wet atomizing treatment of the solution to obtain an extract, wherein the wet atomization treatment comprises (a) high pressure spraying the solution from a high pressure nozzle. Atomization process, (b) supplying air to the atomized solution, (c) discharging in air bubbles formed by supplying air, performing in-liquid plasma treatment, containing fucoxanthin to obtain an extract, characterized by a-law including the relates to a method.

The invention according to claim 2, characterized in that more than 5% of the particles formed by wet atomization process is in the range of particle size 1 [mu] m-100 [mu] m, to a method as claimed in claim 1.

The invention according to claim 3, consisting solely silicofluoride algae and medium-chain fatty acids, the concentration of fucoxanthin from silicofluoride algae is not less 1.5 mg / g or more, even after storage 2 months fucoxanthin storage rate 78 It relates to a fucoxanthin- containing extract produced by the method according to claim 1 or 2 , characterized in that it is% or more.

The invention according to claim 4 relates to a food or cosmetic composition comprising the fucoxanthin- containing extract according to claim 3 .

According to the invention of claim 1, by extracting the fucoxanthin of silicofluoride algae in a medium-chain fatty acid, concentrated fucoxanthin the solvent was evaporated, without purification, is added directly to the food and cosmetic compositions be able to.
To disrupt the cell walls of silicofluoride algae by treating atomization can be extracted efficiently fucoxanthin.

Furthermore, according to the invention of claim 1, a solution prepared by adding medium-chain fatty acids silicofluoride algae were ground to destroy the cell walls of silicofluoride algae by high-pressure injection from the high-pressure nozzles, by atomization to a micro-sized can be released into the medium-chain fatty acid most fucoxanthin in the cell, the result can be extracted fucoxanthin maximum amount from silicofluoride algae as.
By subjecting the atomized solution to in-liquid plasma treatment, reaggregation is prevented by the action of electrostatic repulsion, and the dispersion stability of fucoxanthin in medium-chain fatty acids is improved. There is also an effect of destruction or sterilization of bacteria due to collision of charged particles in plasma.
Furthermore, by supplying air to the atomized solution prior to the in-liquid plasma treatment, it becomes easy to create an environment susceptible to discharge and spread the action of electrostatic repulsion to the particles in the solution.
By using a medium-chain fatty acid , that is, an edible oil, the finally obtained extract can be added as it is to a food or cosmetic composition.

Further, the invention according to claim 1, including extract extract is obtained from silicofluoride algae Fukokisanchi down.

In addition, the invention according to claim 1, extract containing a high concentration of fucoxanthin compared to the extract obtained in the conventional extraction method is obtained from silicofluoride algae.

According to the invention according to claim 2, contained in the cell of silicofluoride algae by more than 5% of the particles formed by the atomization process is within the range of particle size 1 [mu] m-100 [mu] m, to refine the words micro-sized The effect is that fucoxanthin can be extracted more efficiently.

According to the invention of claim 3 state, and are the concentration of fucoxanthin 1.5 mg / g or more from silicosis algae, the Der Rukoto saving rate is 78% or more fucoxanthin be stored for 2 months A fucoxanthin- containing extract can be provided, which is characterized.

According to the invention as set forth in claim 4 , the food or cosmetic composition containing the fucoxanthin- containing extract as set forth in claim 3 leads to prevention of arteriosclerosis and lifestyle-related diseases if it is ingested as a food, and cerebral thrombosis And reduce the risk of stroke, etc., and exert antioxidative action, antitumor action, nerve cell protective action, and blood sugar level elevation suppressive effect. When used as a cosmetic composition, it produces a skin-beautifying effect and an anti-aging effect.

Fat-soluble component
Hereinafter, the fat-soluble component according to the present invention will be described.

  The fat-soluble components obtained by the extraction method of the present invention include carotenoids such as fucoxanthin and β-carotene, and fatty acids such as EPA.

  Fucoxanthin is CAS number 3351-86-8 and has a structure shown in the following formula (Formula 1).

  (beta) -carotene is CAS number 7235-40-7, and has a structure shown to the following Formula (chemical formula 2).

  EPA is CAS No. 10417-94-4 and has a structure shown in the following formula (Chemical formula 3).

  In the present invention, the extract containing fucoxanthin, EPA, β-carotene can be an extract from algae or the like.

<Algae>
The algae used in the present invention is not particularly limited as long as it contains fucoxanthin, but fucoxanthin is present in brown algae and other unequal hair algae to exhibit a brown-olive color and is photosynthetic in chloroplasts. It functions as an auxiliary dye. In particular, most of the carotenoids in brown algae and diatoms are fucoxanthins. Thus, seaweeds belonging to brown algae and diatoms belonging to diatoms, which contain relatively high amounts of fucoxanthin, are preferred.

Among them, from the viewpoint of resource amount and market circulation, Honda straws such as Honda straw (Sargassum fulvellum) and Akamoku (Sargassum horneri), tilapia, sea turtles, seaweeds, corns and mozuku are more preferable. As long as the content of fucoxanthin is abundant, microalgae and the like may be used.
Honda Walla is a kind of seaweed of the brown alga Hondalaaceae, and is distributed in Honshu, Shikoku, Kyushu, Korean Peninsula, etc. In the first grade, it will be 1-2 m in length. It has a soft texture, leaves are lancet shaped and there are cuts. It becomes buoyant and becomes a flowing algae by having an oval or ovate-shaped air bubble. It has been used for a long time as food, fertilizer and ornaments.
Akamoku is a seaweed that belongs to the brown alga Hondawara ssp. It is distributed in Hokkaido (except the eastern part) and in a deep-sea zone (shallow sea) of the whole of Japan, and also in the Korean peninsula, China and northern Vietnam. It is a first grader and grows from autumn to winter, reaching 4 to 7 m in length. It is a dimorphism (rarely there are individuals of the dimorphism).

These algae may be either natural or aquaculture, and may be used singly or in combination of two or more. In addition, the seaweed may be seaweed as collected from the sea, frozen seaweed, salted seaweed, dried seaweed, water washing treatment, hot water treatment, acid water washing, alkaline water washing, Any of the processed state such as disconnection processing can be used.

<Low polar solvent>
In the present invention, the low polarity solvent is not particularly limited, but medium chain fatty acid, soybean oil, rapeseed oil, safflower oil, corn oil, cottonseed oil, sesame oil, olive oil, coconut oil, palm oil, palm oil, chia seed oil, sunflower oil, rice oil and Contains grape seed oil. It can be selected from the above according to the purpose of use.
A fatty acid is a long chain hydrocarbon monovalent carboxylic acid, and in general, one having 5 to 12 carbon atoms is called a medium chain fatty acid. Medium-chain fatty acids are abundant in dairy products, palm oil and coconut oil. Saturated fatty acids have less oxidizing odor and less turbidity than unsaturated fatty acids, and thus can be suitably used in the present invention. Medium-chain fatty acids are easily absorbed in the body because they have high water solubility compared to long-chain fatty acids, and they are suitable for use in the present invention because they have low oxidative odor.

<Extraction of fat-soluble components from algae>
In the present invention, the method of extracting a lipid-soluble component from algae comprises (1) a step of grinding algae, (2) a step of preparing a solution obtained by adding a low polarity solvent to the crushed algae, (3) the solution Wet atomization to obtain an extract.

In the method of extracting a lipid-soluble component from algae of the present invention, a low polarity solvent is added to crushed algae, and then wet atomization treatment is performed. After crushing the algae, further atomization treatment destroys the algal cell wall and releases most of the components contained in the algal cells into the low polarity solvent. This makes it possible to extract more fat-soluble components than adding algae in the solvent without atomization as in the prior art. In particular, it is suitable as a method for extracting a component having a small content in the cell (content in the cell is 0.2 mg / g or less per wet weight) such as fucoxanthin, EPA and β-carotene.
There is no need to separate the fat-soluble component from the polar solvent as in the case of using a polar solvent such as water by performing "wet-pulverizing treatment" in which the fine particle processing is performed in the low polar solvent, and the fat-soluble component Since it is dissolved in a low polarity solvent, the extract after extraction can be used as it is in food and cosmetic compositions. Since the concentration of the fat-soluble component in the extract is high (approximately 1.5 mg / g) by the atomization treatment, it can be used as it is in food and cosmetic compositions without undergoing a concentration step.
In addition, when it is desired to extract a fat-soluble component such as fucoxanthin at a higher concentration (for example, 5-10 mg / g), algae are further added to the extract obtained as a result of the wet atomization treatment once to obtain wet atomization again. By repeating the process of treating, it is possible to obtain an extract containing a fat-soluble component at a higher concentration (for example, 5 to 10 mg / g).

(Step of crushing algae)
It is desirable to crush the algae roughly as a pretreatment for wet atomization. By crushing roughly in advance, the time required for the wet atomization process can be shortened, and the load imposed on the apparatus for performing the wet atomization process can be reduced.
The apparatus for grinding is not particularly limited, and grinding may be performed using a mill, or may be performed using pressure, or may be impact grinding with a grinding blade. As a grinding mechanism, a ball mill, a rod mill, a SAG mill, an autogenous grinding mill, a high pressure grinding roll, a vertical axis impactor mill, a jet mill and the like can be mentioned.
The particle size of the algae after crushing is preferably 1 mm or less.

(Wet atomization process)
By carrying out atomization treatment in a state of containing a low polar solvent, fat-soluble components in algal cells are released into the low polar solvent.
Examples of the wet atomization treatment include a wet jet mill, dispersion by ultrasonic vibration, and wet mechanical contact grinding using a grinding medium such as a bead mill. In the present invention, a wet jet mill can be more preferably employed.

The wet jet mill is a method using a water jet technology, and is developed as an atomization method without causing contamination of particles without breaking the particles themselves by injecting a raw material solution under high pressure from a high pressure nozzle. In the present invention, algae pre-treated and coarsely crushed in advance, to which a low polarity solvent has been added, is high-pressure jetted from a high-pressure nozzle.
The pressure from the high pressure nozzle is not particularly limited, but may be adjusted between 15 and 500 MPa, and the operation of high-pressure injection of the solution from the high pressure nozzle at a predetermined pressure may be repeated multiple times if necessary. By using it in this manner, it is possible to easily make the algae into particles of a desired median diameter.

In the wet atomization treatment, in addition to the above steps, a step of supplying air to the atomized solution, and discharging in air bubbles formed by supplying air, plasma treatment in liquid, a fat-soluble component And B. obtaining an extract containing
That is, the dispersion by physical action of the high-pressure injection processing apparatus and the reforming action by the in-liquid plasma process are continuously performed, and the synergetic effect of these elements prevents reaggregation and improves the dispersion stability. There is also an effect of destruction or sterilization of bacteria due to collision of charged particles in plasma.
Preferably, air is supplied prior to the in-liquid plasma treatment. By supplying air, the effect of plasma treatment is uniformly spread in the solution because it is discharged in the air bubbles.

The apparatus for the wet atomization process may include a pump for refluxing the plasma-treated extract. By providing the pump, the operation of performing high-pressure injection and in-liquid plasma processing is repeated multiple times.
The number of repetitions is preferably 1 to 30 times. Even if it exceeds 30 times, the extraction efficiency of the fat-soluble component is not further improved.

At least 5% of the particles formed by the above-mentioned atomization treatment are in the range of 1 μm to 100 μm in particle diameter. By forming micro-sized particles by atomization treatment, fat-soluble components contained in algal cells can be extracted more efficiently.
Furthermore, the extract contains a high concentration of fat-soluble components. For example, if it is the said method, the density | concentration of fucoxanthin will be 1.5 mg / g or more.

  The extract with high concentration of a fat-soluble component of the present invention containing the fat-soluble component obtained by the above method as an active ingredient can be used for various uses.

  The extract with high fat-soluble ingredient concentration of the present invention is a food additive such as soft drink, dairy product (processed milk, yogurt), confectionery product (jelly, chocolate, biscuit, gum, tablets) or supplement etc. It can also be blended in various food and drink products.

  When used as a food additive, the addition amount thereof is not particularly limited, and may be appropriately determined according to the type of food. As an example, the dry weight of the above-mentioned extract may be added so that the content is in the range of about 1 to 50 mg / g.

  Examples of the above-mentioned food use include breads, confectionery, noodles, cooked rice, potatoes, pasta, dressings, mousse, jelly and soup.

  The form of the food is not particularly limited, but in the case of, for example, a bar-type food, a block-type food or a tearback-type food, oral intake is facilitated. Moreover, as beverages, for example, juices, milk beverages, alcoholic beverages, tea beverages and the like can be mentioned. In addition, food for specified health use, nutritional supplement, nutritional agent, and so-called health food may be used.

For cosmetic use, it is intended to prevent skin dullness, rough skin, prevent stains, improve wrinkles, rejuvenate skin, make up of milky lotion, cream, lotion, essence, pack, cleansing agent, foundation, blush, lipstick etc. Applications include scalp cosmetics such as cosmetics, hair tonics, hair tonics, shampoos, and rinses, dispersions, ointments, solutions, aerosols, and patches.

  Hereinafter, the effects of the present invention will be made clearer by describing examples of the present invention. However, the present invention is not limited to the following examples.

Production of an extract containing a high concentration of a fat-soluble component (Example 1)
The diatom (Phaeodactylum tricornutum) used as a raw material was cultured using f / 2 medium. The culture was performed for 10 days in 100 μmol m ⁻² S ⁻¹ (light period 24 hours) using a 500 L water tank (made of polycarbonate) in a constant temperature room at 22 ° C.
The obtained culture solution was subjected to centrifugation (3,000 rpm, 10 minutes), the supernatant was removed, and diatoms were collected as a precipitate. The recovered diatoms were mixed with medium-chain fatty acids (Panacet 810, manufactured by NOF Corporation) at a weight ratio of 1: 1.
The mixture was then subjected to a wet jet mill at a high pressure nozzle pressure of 250 MPa four times to obtain an extract.
The obtained extract was centrifuged (3000 rpm, 10 minutes, etc.) to recover the supernatant containing the fat-soluble component as an extract in which fatty acids are dissolved.
About 1 g of the extract was dissolved in methanol, treated with an ultrasonic device for 5 minutes, and then adjusted to 100 mL. This is referred to as Example 1.

(Comparative example 1)
The diatom (Phaeodactylum tricornutum) used as a raw material was cultured using f / 2 medium. The culture was performed for 10 days in 100 μmol m ⁻² S ⁻¹ (light period 24 hours) using a 500 L water tank (made of polycarbonate) in a constant temperature room at 22 ° C.
The obtained culture solution was subjected to centrifugation (3,000 rpm, 10 minutes), the supernatant was removed, and diatoms were collected as a precipitate.
13.80 g of the recovered sample was sonicated with an equal weight of ethanol for 10 minutes. The resulting mixture was centrifuged (3000 rpm, 10 minutes) to recover the supernatant containing the fat-soluble component as an extract in which fatty acids are dissolved. About 1 g of the extract was dissolved in methanol, treated with an ultrasonic device for 5 minutes, and then adjusted to 100 mL. This is Comparative Example 1.

2. In order to measure the extraction efficiency of fucoxanthin of Comparative Example 1 and Comparative Example 1 of extraction efficiency, liquid chromatography analysis using an HPLC column was performed. The conditions of analysis are as follows.

・ Apparatus LC-20AT, and UV-visible spectrophotometer SPD-20A
(Made by Shimadzu Corporation)
・ Column Tosoh TSK-Gel ODS-80TM
4.6 4.6 mm × 250 mm (made by Tosoh Corporation)
Mobile phase methanol: water = 90: 10
・ Flow rate 1.0 ml / min ・ Column temperature 40 ° C
・ SPD-20A analysis wavelength 450nm

  Thereafter, standard fucoxanthin, Example 1 and Comparative Example 1 were analyzed under the same conditions and apparatus to measure the concentration of fucoxanthin. The results are shown in Table 1 below.

The results described in Table 1 above indicate that the fucoxanthin concentration in Example 1 obtained by the extraction method of the present invention is higher than that in the conventional method (extraction by ultrasonication in ethanol, Comparative Example 1). Indicated.
That is, it was found that the extraction method of the present invention has a higher fucoxanthin extraction efficiency than conventional ethanol extraction.

3. Relationship between the number of wet atomization processes and the extraction efficiency (Comparative Example 2 and Example 2-4)
The diatom (Phaeodactylum tricornutum) used as a raw material was cultured using f / 2 medium. The culture was performed for 10 days in 100 μmol m ⁻² S ⁻¹ (light period 24 hours) using a 500 L water tank (made of polycarbonate) in a constant temperature room at 22 ° C.
The obtained culture solution was subjected to centrifugation (3,000 rpm, 10 minutes, etc.), the supernatant was removed, and diatoms were collected as a precipitate.
2,400 g of the recovered diatoms were mixed with 400 g of medium-chain fatty acid (Panacet 810, manufactured by NOF Corporation), and then subjected to wet atomization treatment zero times, once, three times, and four times. These were designated as Comparative Example 2 and Examples 2, 3 and 4, respectively. The concentration of fucoxanthin at this time is shown in Table 2 below.

  It was shown that the extraction efficiency of the fat-soluble component is improved by repeating the wet atomization treatment in the above extraction method a plurality of times by adding diatoms.

Simple accelerated test (storage stability test of fucoxanthin)
The present invention was stored under refrigeration (-20.degree. C.), refrigeration (4.degree. C.), normal temperature (25.degree. C.) and dark place, and the fucoxanthin concentration after 1 month was measured.

(Example 5)
The diatom (Phaeodactylum tricornutum) used as a raw material was cultured using f / 2 medium. The culture was performed for 10 days in 100 μmol m ⁻² S ⁻¹ (light period 24 hours) using a 500 L water tank (made of polycarbonate) in a constant temperature room at 22 ° C.
The obtained culture solution was subjected to centrifugation (3,000 rpm, 10 minutes, etc.), the supernatant was removed, and diatoms were collected as a precipitate.
The same weight of medium-chain fatty acid was added to about 1 kg of the collected diatoms, and treatment with a wet jet mill was repeated four times with the pressure of the high pressure nozzle set to 250 MPa. This is taken as Example 5.

The fucoxanthin concentration at the start of the accelerated test of Example 5 was 2.86 mg / g. They were stored under refrigeration (−20 ° C.), refrigeration (4 ° C.) and normal temperature (25 ° C.) in the dark, respectively.
After storage for one month, the fucoxanthin concentration in Example 5 was 2.35 mg / g in frozen storage, 2.25 mg / g in refrigerated storage, and 2.30 mg / g in normal temperature storage. Similarly, the fucoxanthin concentration of Example 5 after storage for 2 months was 2.42 mg / g in frozen storage, 2.34 mg / g in refrigerated storage, and 2.30 mg / g in normal temperature storage.
That is, even after 2 months, the preservation rate was 82% or more for freezing, 78% or more for refrigeration, and 80% for normal temperature.
About 80% of fucoxanthin was found to be preserved by any preservation method.

The high storage rate is considered to be due to the physical action of the wet atomization treatment.
That is, when fucoxanthin is extracted by the extraction method of the present invention, it is possible to obtain an extract containing a high concentration of a fat-soluble component having a high storage rate of fucoxanthin.

The present invention can be expected to be a skin-care effect and an anti-aging effect when used as a cosmetic, and can be expected to prevent arteriosclerosis and lifestyle-related diseases, to have an antitumor effect and a nerve cell protection action when taken as a food. It can be suitably used as an additive for various food and drink such as cosmetics, soft drinks, dairy products, confectionery products or supplements.

Claims (4)

Fucoxanthin from silicofluoride algae A method for extracting at a concentration of at least 1.5 mg / g,
(1) a step of pulverizing the silicofluoride algae,
(2) a step to prepare a solution prepared by adding a medium chain fatty acid into crashed silicofluoride algae, and (3) obtaining an extract with the solution was treated wet atomization,
Wet atomization process,
(A) a step of subjecting the solution to high pressure injection from a high pressure nozzle and atomizing treatment;
(B) supplying air to the atomized solution,
(C) discharging in air bubbles formed by supplying air, performing in-liquid plasma treatment to obtain an extract containing fucoxanthin ,
The features and-law including, method. Characterized in that more than 5% of the particles formed by wet atomization process is in the range of particle size 1 [mu] m-100 [mu] m, method according to claim 1. Consisting solely silicofluoride algae and medium-chain fatty acids, the concentration of fucoxanthin from silicofluoride algae is not less 1.5 mg / g or more, and wherein the 2-month storage and fucoxanthin storage modulus even is 78% or more A fucoxanthin- containing extract produced by the method according to claim 1 or 2 . A food or cosmetic composition comprising the fucoxanthin- containing extract according to claim 3 .