KR20200031990A - Composition for inhibiting a formation of biofilm - Google Patents

Abstract

The present invention relates to a composition which suppresses generation of a biofilm in an oral cavity or on a surface of a tooth while being hypoallergenic. More specifically, the present invention relates to a composition, specifically an oral composition, which coats a tooth by containing vegetable oil to inhibit formation of a biofilm, prevent tooth coloring, alleviate irritation due to a surfactant used as a foaming agent, and be effective in moisturizing lips.

Description

Composition for inhibiting a formation of biofilm}

The present invention relates to a composition that suppresses the formation of biofilms in the oral cavity or on the tooth surface while being hypoallergenic, more specifically, by coating the teeth by containing vegetable oil to inhibit biofilm formation and prevent tooth coloring, It relates to a composition that is effective in moisturizing the lips while reducing the irritation caused by the surfactant used as a foaming agent, specifically, a composition for oral cavity.

Tooth is a calcium phosphate (Calcium phosphate) mineral, and related diseases are largely decayed and periodontal disease. Among them, tooth decay is the largest part of dental diseases from children to adults, and the incidence is increasing. According to a report by the Korean Dental Association, more than 90% of children experience dental caries, and more than 80% of adults have gum disease. The main cause of this disease is infection by microorganisms in the oral cavity, which is caused by the interaction of bacteria, food, and saliva. That is, the nutrients and moisture necessary for the growth of bacteria in the oral cavity are continuously supplied by food, saliva, gingival fissure, etc., and the environment in the oral cavity has a temperature (37 ° C. pH (near neutral) suitable for microorganisms to develop.

Representative microorganisms that cause periodontal disease include Streptococcus mutans and Prevotella intermedia. These microorganisms cause microbial metabolism that produces sucrose present in food into glucose and fructose, forming insoluble glucan, a polymer of glucose, on the tooth surface. In this process, glucan attaches to other teeth and other microbes in the oral cavity to form a biofilm, ie, a dental plaque. The lactic acid accumulated by the lactic acid bacteria including Streptococcus mutans inside the formed plaque dissolves the enamel on the tooth surface, causing cavities. In addition, alveolar bone is dissolved by the products resulting from the proliferation of various bacteria, so that periodontal disease occurs.

Periodontal disease is often referred to as pungchi, depending on the severity of the disease is divided into gingivitis (gingivitis) and periodontitis (periodontitis). Periodontitis is a periodontal disease of relatively light and rapid recovery that is limited to the gums, ie, soft tissue, and is called gingivitis.

In addition, there is a V-shaped gap between the gingiva (gum) and the teeth. Periodontal disease refers to the damage of the periodontal ligament and adjacent tissues by bacteria attacking the lower part of the gum line of this groove. As inflammation progresses and more tissue is damaged, the groove develops into a periodontal pocket, and the deeper the periodontitis, the deeper the depth of the periodontal pocket. As the periodontal sap deepens, the periodontal ligament becomes inflamed and bone loss occurs.

In order to suppress the dental caries and periodontal disease, the antibacterial substances sodium bicarbonate (NaHCO ₃ ), triclosan (Triclosan, C ₁₂ H ₇ Cl ₃ O ₂ ), cetylpyridinium chloride, polyphosphate (Polyphosphate) and sodium fluoride (NaF) ), Antibiotics such as Vancomycin, Chlorhexidine and Spiramicin, sodium chloride (NaCl), Allantoine Chlorohydroxy Aluminum, Aminocaproic Acid, tocopherol acetate acetate), or organic / inorganic fluorine has been used. However, although the above methods are effective in preventing tooth decay, their use is limited due to the disadvantages of causing vomiting, diarrhea, and resistance to antibiotics. In particular, in the past, antibiotics have been generally used for bacterial infection diseases such as tooth decay and periodontitis, but antibiotics not only harm all of the beneficial bacteria in the human body, but when overdosed, the appearance of resistant bacteria, deterioration of the patient's immune capacity, and consequent infection of diseases It causes side effects such as chronicization and mycosis.

In addition, a surfactant such as sodium lauryl sulfate is widely used as a foaming agent for toothpaste, but it is also known as a component causing irritation and inflammation.

Therefore, there is still a need for an oral composition having excellent safety while effectively suppressing the formation of a biofilm, that is, a plaque, on the tooth surface.

1. U.S. Patent No. 9,114,097 (registered on August 25, 2015)

Accordingly, the present inventors have effectively prevented the formation of biofilm, prevented tooth coloring, and prevented irritation caused by surfactants and effectively moisturized lips by including vegetable oil. It was confirmed that it can provide a toothpaste composition, and the present invention was completed.

Accordingly, an object of the present invention is to provide a composition for oral cavity having excellent safety while suppressing tooth decay and periodontal disease by inhibiting biofilm formation.

In order to achieve the above object, the present invention is a vegetable oil; At least one of higher alcohol and higher fatty acid; And it provides a composition for inhibiting biofilm formation containing at least one of anionic surfactant and nonionic surfactant as an active ingredient.

Since the composition of the present invention does not contain an antibacterial agent having a safety problem, it can suppress the formation of a biofilm when it strikes the body safely and effectively, and also prevents tooth coloring by coffee, tea, etc., and prevents irritation by a surfactant. It relieves and can effectively act on moisturizing lips.

The composition according to the invention comprises vegetable oils; At least one of higher alcohol and higher fatty acid; And anionic surfactants and nonionic surfactants.

The vegetable oil used in the present invention is coated with teeth to suppress biofilm formation, to relieve irritation caused by surfactants, etc., and is effective for protecting lips, coconut oil, olive oil, sunflower seed oil, grape seed oil, castor oil, etc. It is one or more selected from.

In the present invention, the vegetable oil is contained in an amount of 5 to 50% by weight, preferably 10 to 40% by weight, more preferably 15 to 30% by weight based on the total weight of the composition. If the content is less than 5% by weight, it is difficult to exhibit a biofilm suppression and tooth coloration prevention effect by tooth coating, and if the content is more than 50% by weight, the stability of the formulation is lowered, and the feeling of use such as foaming power and freshness is deteriorated.

The composition according to the present invention uses at least one of higher alcohol and higher fatty acid as a formulation agent in order to realize a formulation having a soft feeling like fresh cream. In the present invention, the higher alcohol is at least one selected from stearyl alcohol and cetyl alcohol, and the higher fatty acid is at least one selected from stearic acid and cetyl acid.

In the present invention, at least one of higher alcohol and higher fatty acid is contained in an amount of 3 to 15% by weight, preferably 5 to 12% by weight, more preferably 6 to 10% by weight based on the total weight of the composition. If the content is less than 3% by weight or more than 15% by weight, paste formulation of the toothpaste is not formed, and formulation stability is poor or irritation in the oral cavity is present.

The composition according to the present invention uses a mixture of one or more surfactants, more preferably one or more anionic surfactants and one or more nonionic surfactants, among the anionic surfactants and nonionic surfactants for dense foam formation. In the present invention, sodium lauryl sulfate or the like may be used as the anionic surfactant, and sorbitan stearate or lauryl glucoside or the like may be used as the nonionic surfactant.

In the present invention, the surfactant is contained in an amount of 0.5 to 5% by weight, preferably 1 to 4.5% by weight, more preferably 1.5 to 4% by weight based on the total weight of the composition. If the content is less than 0.5% by weight or more than 5% by weight, the paste formulation of the toothpaste is not well formed, and the formulation stability is poor or irritation in the oral cavity is present.

The composition according to the present invention is not particularly limited in its formulation as long as it is intended to suppress the formation of a biofilm upon tooth. Specifically, it may be formulated as a product for oral cleansing, for example, toothpaste, mouthwash, mouth spray, tissue for mouthwash, tooth whitening agent, and preferably toothpaste.

The composition according to the present invention may contain abrasives, wetting agents, auxiliary foaming agents, binders, sweeteners, pH adjusting agents, preservatives, active ingredients, fragrances, brighteners, pigments, solvents, etc., which are commonly used depending on the formulation and purpose of use.

The composition according to the present invention is a vegetable oil as described above; Higher alcohol or higher fatty acid; And by containing an anionic or nonionic surfactant as an active ingredient, the effect of inhibiting the formation of a biofilm is excellent, it is also effective in preventing tooth coloring by coffee, tea, etc., and the irritation caused by the use of the surfactant is also significantly reduced. In addition, it has the same texture as fresh cream and is gently applied to the teeth. When used, the foam is dense, and after use, it gives a moisturizing feeling to the lips as well as the teeth to give a soft feeling.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to test examples and manufacturing examples. However, these test examples and manufacturing examples are provided for illustrative purposes only to aid understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Reference Example] Preparation of Examples and Comparative Examples

Toothpaste compositions of Comparative Examples 1 to 12 and Examples 1 to 23 with the compositions of Tables 1 to 5 below were prepared by conventional methods in the industry.

Raw material Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 menstruum water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 Fluoride SMFP 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 Antibacterial Cetylpyridinium chloride 0.05 Sweetener Sodium saccharin 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Wetting agent glycerin 20 20 20 20 20 20 20 20 Advanced
Alcohol
&
Advanced
fatty acid Cetyl alcohol 3 3 3 One 5 3 3 3 Stearyl alcohol 4 4 4 One 8 4 4 4 Stearic acid 2 2 2 0.5 3 2 2 2 oil Coconut oil 30 30 30 30 3 Olive oil Spices Flavor One One One One One One One One Thickener Hydrated silica 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 Sodium carboxymethyl cellulose One One One 0.3 0.3 0.3 0.3 0.3 Surfactants Sorbitan stearate 0.4 0.4 0.4 3 0.4 Sodium lauryl sulfate 2 2 2 2 3 2 Sodium lauroyl glutamate 2

Raw material Comparative Example 9 Comparative Example 10 Comparative Example 11 Comparative Example 12 menstruum water To 100 To 100 To 100 To 100 Fluoride SMFP 0.76 0.76 0.76 0.76 Antibacterial Cetylpyridinium chloride Sweetener Sodium saccharin 0.2 0.2 0.2 0.2 Wetting agent glycerin 20 20 20 20 Advanced
Alcohol
&
Advanced
fatty acid Cetyl alcohol 3 9 3 Stearyl alcohol 4 4 Stearic acid 2 9 2 oil Coconut oil 55 Olive oil Spices Flavor One One One One Thickener Hydrated silica 2.5 2.5 2.5 2.5 Sodium carboxymethyl cellulose 0.3 One One One Surfactants Sorbitan stearate 3 0.4 Sodium lauryl sulfate 2 2 2 2 Sodium lauroyl glutamate

Raw material Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 menstruum water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 Fluoride SMFP 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 Antibacterial Cetylpyridinium chloride Sweetener Sodium saccharin 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Wetting agent glycerin 20 20 20 20 20 20 20 20 High-grade alcohol
&
High-grade fatty acids Cetyl alcohol 3 3 3 3 3 3 3 3 Stearyl alcohol 4 4 4 4 4 4 4 4 Stearic acid 2 2 2 2 2 2 2 2 Cetyl acid oil Coconut oil 30 5 15 50 Olive oil 30 Sunflower seed oil 30 Grape seed oil 30 Castor oil 30 Spices Flavor One One One One One One One One Thickener Hydrated silica 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 Sodium carboxymethyl cellulose 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Surfactants Sorbitan stearate 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.6 Lauryl glucoside Sodium lauryl sulfate 2 2 2 2 2 2 2 2 Sodium lauroyl glutamate

Raw material Example 9 Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 Example 16 menstruum water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 Fluoride SMFP 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 Antibacterial Cetylpyridinium chloride Sweetener Sodium saccharin 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Wetting agent glycerin 20 20 20 20 20 20 20 20 High-grade alcohol
&
High-grade fatty acids Cetyl alcohol 3 3 3 3 3 One 5 3 Stearyl alcohol 4 4 4 4 4 One 8 4 Stearic acid 2 2 2 2 2 One 2 2 Cetyl acid oil Coconut oil 15 15 15 15 6 30 30 30 Olive oil 15 6 Sunflower seed oil 15 6 Grape seed oil 15 6 Castor oil 15 6 Spices Flavor One One One One One One One One Thickener Hydrated silica 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 Sodium carboxymethyl cellulose 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Surfactants Sorbitan stearate 0.6 0.6 0.6 0.6 0.6 0.6 0.6 0.5 Lauryl glucoside Sodium lauryl sulfate 2 2 2 2 Sodium lauroyl glutamate

Raw material Example 17 Example 18 Example 19 Example 20 Example 21 Example 22 Example 23 menstruum water To 100 To 100 To 100 To 100 To 100 To 100 To 100 Fluoride SMFP 0.76 0.76 0.76 0.76 0.76 0.76 0.76 Antibacterial Cetylpyridinium chloride Sweetener Sodium saccharin 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Wetting agent glycerin 20 20 20 20 20 20 20 High-grade alcohol
&
High-grade fatty acids Cetyl alcohol 3 9 3 3 3 3 Stearyl alcohol 4 4 4 4 4 Stearic acid 2 9 2 2 2 Cetyl acid 2 oil Coconut oil 30 30 30 30 30 30 30 Olive oil Sunflower seed oil Grape seed oil Castor oil Spices Flavor One One One One One One One Thickener Hydrated silica 2.5 2.5 2.5 2.5 2.5 2.5 2.5 Sodium carboxymethyl cellulose 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Surfactants Sorbitan stearate 3 0.6 0.6 2.6 0.6 Lauryl glucoside 0.6 Sodium lauryl sulfate 2 2 2 2 2 Sodium lauroyl glutamate

[Test Example 1] Formulation stability evaluation

The formulations of Examples 1 to 23 and Comparative Examples 1 to 12 prepared in the reference example were evaluated for formulation degree and formulation stability in paste form.

Through the stability evaluation, it was confirmed whether discoloration, deodorization, separation, etc. occurred for 4 weeks in a 0 ° C, 45 ° C, 60 ° C, cycling test (-10 ° C to 45 ° C, maintained for 11 hours).

Specifically, when the formulation in the form of a paste was made, it was evaluated as O, and when not, it was evaluated as X. When separation of the formulation did not occur, it was evaluated as O when the formulation was stable, and as X when separation occurred. Table 6 below shows the evaluation results.

Comparative example One 2 3 4 5 6 7 8 9 10 11 12 Paste form
Formulation O O O X X O O O O O O O Stability such as separation O O O X O X O O X O O O Example One 2 3 4 5 6 7 8 9 10 11 12 Paste form
Formulation O O O O O O O O O O O O Stability such as separation O O O O O O O O O O O O Example 13 14 15 16 17 18 19 20 21 22 23 Paste form
Formulation O O O O O O O O O O O Stability such as separation O O O O O O O O O O O

As shown in Table 6, in the case of Comparative Examples 4 and 5 in which the content of the higher alcohol and higher fatty acid was outside the appropriate range, the formulation in the form of a paste was not well performed, and the comparative example in which the content of the surfactant was less than the proper range In the case of 6 and Comparative Example 9 in which the oil content was outside the appropriate range, separation of the formulation occurred.

Based on the evaluation results, in the following test examples, efficacy verification and experiments were conducted for Comparative Examples 1 to 3, 7, 8, 10 to 12, and Examples 1 to 23.

[Test Example 2] Evaluation of biofilm formation inhibitory effect (In-vitro)

In Comparative Examples 1 to 3, 7, 8, 10 to 12, and Examples 1 to 23 prepared in the reference example, an in-vitro test for evaluating the biofilm formation inhibitory ability in each of the examples is a tooth simulating material. Phosphorus hydroxyapatite disk (Biotek) was used.

a) Streptococcus mutans (KCTC 3065) bacteria were inoculated in Brain Heart infusion broth (Difco, BD Biosciences) and cultured in a 35 ° C incubator (Imperial lll Incubator, Lab Line Instruments Inc.) for 24 hours.

b) The hydroxyapatite disc was sterilized by supporting it in 70% ethanol for 30 minutes, and then washed three times with sterile ionized water.

c) Washed hydroxyapatite discs are placed one by one in a 24-well plate (BD Falcon) and dried completely.

d) Comparative Examples 1 to 3, 7, 8, 10 to 12, and each of Examples 1 to 23 samples were diluted by 20% with ionic water and 1 mL was taken and dispensed into wells containing hydroxyapatite discs and treated for 3 minutes. .

e) The hydroxyapatite disc was rinsed with sterile ionized water to remove the remaining sample.

f) After transferring the hydroxyapatite disc to another 24-well plate, 1 ml of Brain Heart infusion broth containing 1% sucrose was added to each well, and 1 μL of the bacterial culture was inoculated.

g) The 24-well plate inoculated with the bacteria was cultured in a 35 ° C incubator for 3 days to form a biofilm.

h) After 3 days, the growth of bacteria was observed, and after washing the hydroxyapatite disc with sterile ionized water, 1 mL of 0.1% Crystal violet solution (Sigma) was dispensed at 1 mL and stained for 30 minutes.

i) The hydroxyapatite disc was washed with sterile ionized water and then dried completely at room temperature.

j) 1 mL of 70% ethanol was added, and the dye was extracted until the dyed hydroxyapatite disc was discolored to white.

l) After diluting the extract in 1/2 steps, the absorbance was measured using a spectrophotometer (Spectramax 190, Molecular Devices Corporation) (OD 590 nm).

m) By subtracting the value of the blank well from the measured value and selecting the dilution ratio value measured with a value of 2 or less, the ability to inhibit biofilm formation was calculated.

The results are shown in Table 7 below.

Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 7 Comparative Example 8 Comparative example
10 Comparative Example 11 Comparative Example 12 Biofilm inhibitory effect (%) 29.7 49.57 20.4 76.12 30.12 29.56 30.23 30.01 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Example 7 Example 8 Biofilm inhibitory effect (%) 75.71 45.49 50.15 80.01 76.71 75.42 74.65 75.55 Example 9 Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 Example 16 Biofilm inhibitory effect (%) 79.25 78.54 77.77 78.98 80.05 70.01 72.52 72.25 Example 17 Example 18 Example 19 Example 20 Example 21 Example 22 Example 23 Biofilm inhibitory effect (%) 77.87 76.77 74.69 75.59 74.58 75.85 74.89

As shown in Table 7, Comparative Example 1, 3, 10 to 12 without coconut oil and an antibacterial agent, and Comparative Example 8 in which the content of coconut oil was less than an appropriate range was found to have a significantly low biofilm inhibitory effect. Comparative Example 2 including cetylpyridinium chloride, an antibacterial agent that has been used in existing products, also showed a lower biofilm inhibitory effect than the example containing vegetable oil in an amount of 15% by weight or more. On the other hand, in Comparative Example 7 and Examples containing both vegetable oils such as coconut oil and olive oil used as a tooth coating agent in the present invention, both of Comparative Examples 1 and 3 showed that the effect of inhibiting biofilm formation was significantly higher. , Compared to Comparative Example 2 and Example containing the antibacterial agent cetylpyridinium chloride and not containing coconut oil, in Example 2 containing 5% by weight of coconut oil, the antibacterial effect was found to be lower, but coconut It can be seen that in the other examples except Example 2 containing 15% by weight or more of oil and olive oil, the biofilm formation inhibitory effect is similar or better than that of Comparative Example 2.

[Test Example 3] To evaluate the effect of preventing tooth pigmentation (In-vitro)

In Comparative Examples 1, 3 and Examples 1, 2, 4, 6, 10, 13 prepared in the reference example, an in-vitro test for evaluating the ability to inhibit tooth pigmentation was measured. Loxiapatite discs (Biotek) were used.

a) The initial value of the hydroxyapatite disc was measured using ShadeEye-EX (Shofu Dental corporation).

b) Each of the samples of Comparative Examples 1, 3 and Examples 1, 2, 4, 6, 10, 13 was applied to the disk for 3 minutes using a brushing machine, and then washed with running water for 30 seconds.

c) After washing the hydroxyapatite discs in Americano coffee, 3 of them were taken out after 4 hours and 8 hours to measure the change in brightness.

The measurement results are shown in Table 8 below.

Prevention of tooth coloring Comparative Example 1 Comparative Example 3 Example 1 Example 2 Example 4 Example 6 Example 10 Example
13 L value 99.6 99.7 99.6 99.6 99.7 99.6 99.8 99.6 4 hours later 88.5 89.1 90.6 90.6 95.4 90.9 92.9 91.9 8 hours later 80.7 81.3 89.8 83.1 91.2 90.1 90.5 91.1

As shown in Table 8, Comparative Examples 1 and 3 prepared by different types of surfactants included in order to find out whether or not the type of the anionic surfactant affects the prevention of tooth coloration have significant differences in the prevention of coloring. On the other hand, in the case of the examples containing oil, such as coconut oil, it showed a high tooth coloring prevention effect compared to Comparative Examples 1 and 3, which does not contain coconut oil. In particular, the tooth coloring prevention effect showed a significant difference after about 8 hours after applying the coloring material.

[Test Example 4] Oral irritation evaluation (In-vitro)

In Comparative Examples 1, 2, 3, 7 and Examples 1, 2, 4, 6, 10, 13 prepared in the reference example, the oral stimulation degree was measured in the Agar diffusion assay test method (ISO 10993-5: 2009 ( E) Biological evaluation of medical device Part 5 test for in vitro cytotoxicity: Agar diffusion assay).

a) 2 ml of L929 (Mouse fibroblast, ACTT, USA) cells with 10% FBS and 1% penicillin-streptomycin RPMI-1640 medium (Gibco, USA) at a density of 1 × 10 ⁵ per well in a 6-well plate Each was dispensed and incubated for 24 hours at 5% CO ₂ , 37 ° C.

b) After 24 hours, when the cells form a single cell layer of 80% or more in the well, the culture is removed and 1.5% agarose (Sigma-Aldrich), 10% FBS (Gibco), and 0.0033% Neutral red (Sigma-Aldrich) ) Containing 50 ml of phenol-free DMEM (Gibco) medium at 2 ° C was dispensed by 2 ml, and this was solidified at 5% CO ₂ and 37 ° C for 45 minutes.

c) Comparative Examples 1, 2, 3, 7 and Examples 1, 2, 4, 6, 10, 13, each of the test substances diluted 25% in RPMI-1640 medium on an 8 mm diameter paper disk (Advantec, Japan) 15 μl was dispensed and moistened, and the soaked paper disc was placed on the agarose solidified in b), and then cultured at 5% CO ₂ at 37 ° C. for 24 hours.

d) The distance from the paper disc to the edge of the resulting ring was measured using a 15 cm ruler.

The measurement results are shown in Table 9 below.

Oral irritation evaluation Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 7 Example 1 Example 2 Example 4 Example 6 Example 10 Example 13 Agarose bleaching
(mm) 5.67 7.12 0.33 6.13 3.00 4.13 1.23 2.70 2.90 2.55

As shown in Table 9, Comparative Example 3 containing a glutamate-based surfactant showed the lowest oral irritation. On the other hand, when comparing Comparative Example 1 with Examples 1, 2, 4, 6, 10, 13 , These all contain the same amount of sodium lauryl sulfate, but showed a difference in the degree of oral irritation, and it can be confirmed that the irritation is low especially in the case of the Examples. Through this, it can be expected that coconut oil helps to alleviate irritation caused by sulfate-based surfactants.

However, in Comparative Example 7, when the surfactant content exceeds 5% by weight, it can be seen that the effect of alleviating irritation by coconut oil is insufficient.

[Test Example 5] Sensory evaluation

Sensory evaluation was conducted through a specialized panel composed through the evaluation of the feeling of use for toothpaste products. With respect to Comparative Example 1, Example 1, 2, 4, 6, 10, and 13 prepared in the reference example, 10 expert panelists rated 7 products after brushing 7 products 3 times a day for 3 weeks, 3 times each, and then rated them with a score of 9 Was made. The evaluation results are shown in Table 10 below.

Specialized panel: 10 Sensory evaluation Comparative Example 1 Example 1 Example 2 Example 4 Example 6 Example 10 Example 13 Air bubbles 7.2 4.5 5.7 2.1 4.3 4.7 4.8 Refreshing 6.8 6.5 6.6 4.1 6.3 6.2 6.6 Soft 3.1 8.2 4.6 8.6 8.1 8.2 8.4 Moisturizing lips 2.5 7.1 3.5 8.4 7.4 7.5 7.7 Very satisfied: 9 / Average: 5 / Very insufficient: 1

As can be seen from the evaluation results in Table 10, in the case of bubble power, Comparative Example 1, which did not contain oil, was high, and among Examples 1, 2, 4, 6, 10, 13, a relatively small amount of oil was used. In the case of Example 2 containing relatively high, while Example 4 containing a relatively large amount of oil appeared to have low foaming power, it was confirmed that there was a significant correlation between the oil content and foaming power. In terms of softness, Examples 1, 2, 4, 6, 10, and 13 were significantly superior to Comparative Example 1.

In addition, in the case of Examples 1, 2, 4, 6, 10, and 13 containing oil in terms of moisturizing lips, it was found that the moisturizing power was excellent.

From these evaluation results, the oral composition according to the present invention has a dense foam when used and is gently applied to the teeth, and after use can impart a soft feeling to the lips as well as the teeth. Since it is less irritating and effective in protecting lips, it may have a new advantage that is distinguished from conventional oral products.

[Formulation Example 1] Mouthwash

Ingredient name Content (% by weight) Purified water To 100 Sodium fluoride 0.03 Coconut oil 10 Cetyl alcohol 3 Stearic acid 3 Sorbitan stearate 0.6 Sodium lauryl sulfate One Sorbitol solution (70%) 10.00 Sodium saccharin 0.01 Combination spices 0.10

[Formulation Example 2] Teeth whitening agent

Ingredient name Content (% by weight) Polyethylene glycol To 100 glycerin 35.00 Function silicate 20.00 Coconut oil 10 Cetyl alcohol 3 Stearic acid 3 Sorbitan stearate 0.6 Sodium lauryl sulfate One Sodium monofluorophosphate 0.76 Sodium saccharin 0.30 Polyvinylpyrrolidone 1.00 Hydrogen peroxide (35%) 2.14 Combination spices 1.00 Sodium lauryl sulfate 2.00

Claims (10)

At least one vegetable oil selected from the group consisting of coconut oil, olive oil, sunflower seed oil, grape seed oil and castor oil; At least one of higher alcohol and higher fatty acid; And one or more of anionic surfactants and nonionic surfactants; a composition for inhibiting biofilm formation. The composition for inhibiting biofilm formation according to claim 1, wherein the vegetable oil is contained in an amount of 5 to 50% by weight based on the total weight of the composition. The composition for inhibiting biofilm formation according to claim 1, wherein the higher alcohol is at least one of stearyl alcohol and cetyl alcohol, and the higher fatty acid is at least one of stearic acid and cetyl acid. The composition for inhibiting biofilm formation according to claim 1, wherein at least one of the higher alcohol and higher fatty acid is contained in an amount of 3 to 15% by weight based on the total weight of the composition. The composition for inhibiting biofilm formation according to claim 1, wherein the anionic surfactant is sodium lauryl sulfate, and the nonionic surfactant is sorbitan stearate or lauryl glucoside. The composition for inhibiting biofilm formation according to claim 1, wherein the surfactant is included in an amount of 0.5 to 5% by weight based on the total weight of the composition. The composition for inhibiting biofilm formation according to claim 1, wherein the biofilm is a biofilm formed in an oral cavity or on a tooth. According to claim 1, wherein the composition is a composition for inhibiting biofilm formation, characterized in that to prevent tooth pigmentation. A cleaning product for oral cavity made by formulating the composition for inhibiting biofilm formation according to any one of claims 1 to 8. 10. The oral care product of claim 9, which is a toothpaste, mouthwash, mouth spray, tissue for mouthwash, or tooth whitening agent.