KR20200025389A - A device incluing microbeads capable of controlling pH of a sample - Google Patents

Abstract

The present invention relates to a device capable of adjusting the pH of a sample and, more specifically, provides a device comprising microbeads capable of adjusting the pH of a sample to a predetermined value. Also, provided is the device which comprises, in each of a plurality of channels, microbeads enabling the adjustment to different pH values, and thus enables the adjustment to a plurality of different pH values simultaneously upon injection of the sample.

Description

A device incluing microbeads capable of controlling pH of a sample}

The present invention relates to a device capable of adjusting the pH of a sample, and more particularly, to a device including microbeads capable of adjusting a sample to a desired pH, and also to microbeads that can be adjusted to different pH in each of a plurality of channels. Including, relates to a device that can be adjusted to a plurality of different pH at the same time by the injection of the sample.

In general, in order to adjust the pH, the pH value obtained by adding an acid or base solution to a sample is measured by a pH meter or a pH paper sensor, and a process of optimizing for reaching a desired pH value is performed.

In the conventional automatic pH control device, the process of observing the pH of a sample in real time through an electrochemical device by continuously adding an acid or a base is automated by using a valve device. Therefore, it is uneconomical.

On the other hand, for the conventional pH adjustment, a series of processes that measure the pH value obtained by adding an acid or base solution to a sample with a pH meter or a pH paper sensor and perform until a desired pH value is achieved are labor intensive and long time. The disadvantage is that it takes a lot of money. Therefore, to solve the problems in the series of optimization process for the conventional pH control to provide an automated device that can adjust the sample to multiple pH more easily and quickly.

Apparatus comprising microbeads capable of pH adjustment of a sample according to an embodiment of the present invention:

A channel filled with microbeads capable of pH adjustment of the sample;

An inlet through which the sample can be injected into the channel; And

And an outlet through which the discharge of the sample with pH adjustment is made in the channel,

The injected sample may be adjusted to the pH of the microbeads in the channel.

In addition, in the device comprising a micro-bead capable of adjusting the pH of the sample according to an embodiment of the present invention, a salt (salt) on the surface of the micro-bead so that the injected sample can be adjusted to the desired pH in the channel This may be coated.

In addition, in the device comprising a micro-beads capable of adjusting the pH of the sample according to an embodiment of the present invention, the injected sample is pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7 , pH 8, pH 9, pH 10, pH 11, pH 12, pH 13 may be adjusted to any one.

In addition, in the device comprising a micro-beads capable of adjusting the pH of the sample according to an embodiment of the present invention,

And a frit between the outlet and the region filled with the microbeads in the channel,

The sample passing through the microbeads is discharged through the frit to the outlet, but the microbeads may be prevented from exiting the outlet.

In addition, in the device comprising a micro-beads capable of adjusting the pH of the sample according to another embodiment of the present invention,

The channel is composed of a plurality,

Each of the plurality of channels is filled with micro beads having a different pH from each other,

Each channel may be connected to each inlet and each outlet.

In addition, the apparatus including a micro-bead capable of adjusting the pH of the sample according to another embodiment of the present invention:

A main injection unit into which a sample is injected; And

Further comprising a main passage connecting the main injection portion and each injection hole, the main passage portion having an aliquot structure;

Samples injected into the main injection portion may be distributed to the respective channels through the main passage portion, and may be adjusted to the pH of the microbeads filled in the respective channels.

In addition, in the device comprising a micro-bead capable of adjusting the pH of the sample according to another embodiment of the present invention, each channel is pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7 , microbeads having pH 8, pH 9, pH 10, pH 11, pH 12, and pH 13.

In addition, in an apparatus including a micro-bead capable of adjusting the pH of the sample according to another embodiment of the present invention, further comprising an injection member connected to the main injection portion, the injection member further comprises a piston, One end of the injection member may be connected to the main injection part, and a piston may be positioned at the other end of the injection member, and the piston may be pushed into the injection member to move the injected sample into the respective channels.

In addition, in the apparatus including a micro-beads capable of adjusting the pH of the sample according to another embodiment of the present invention, the sample injected into the inlet may be neutral.

In addition, it is possible to provide a micro-beads used in the device comprising a micro-beads capable of pH adjustment of the sample according to the embodiments of the present invention.

According to the present invention, the microbeads can be adjusted to a desired pH more easily and quickly by including microbeads for pH adjustment in the channel, and each of the microbeads provided with a plurality of channels can be adjusted to a plurality of pH values. In case of a single sample injection, there is an advantage that can be adjusted to multiple desired pH at the same time.

1 is a schematic diagram of a device including microbeads capable of pH adjustment of a sample according to an embodiment of the present invention.
FIG. 2 is a schematic diagram of a device including microbeads capable of multiple pH adjustment of a sample, with some modification of the device of FIG.
FIG. 3 is a schematic diagram of a device including microbeads capable of multiple pH adjustment of a sample, embodying the device of FIG. 2 in one embodiment.
Figure 4 is a graph showing the results of the pH change experiment of the sample in the experimental example of the present invention.

Hereinafter, an apparatus 1 including microbeads capable of pH adjustment of a sample according to an embodiment of the present invention will be described in detail. The accompanying drawings show exemplary forms of the present invention, which are provided to explain the present invention in more detail, and the technical scope of the present invention is not limited thereto.

In addition, irrespective of the reference numerals, the same or corresponding components will be given the same reference numerals, and redundant description thereof will be omitted, and the size and shape of each illustrated member may be exaggerated or reduced for convenience of description. have.

1 is a schematic diagram of an apparatus 1 including microbeads capable of pH adjustment of a sample according to an embodiment of the present invention. Device (1) comprising a micro-bead capable of adjusting the pH of the sample according to an embodiment of the present invention, the injection port 10 is a sample is injected, the channel 20 is a pH control of the injected sample, pH control is And an outlet 30 through which the discharged sample is made. The sample injected into the injection hole 10 may be, for example, an aqueous solution.

The channel 20 includes a plurality of micro beads 100 to adjust the pH of the injected sample. A salt is coated on the surface of the micro beads 100 so that the injected sample can be adjusted to a desired pH of pH 0 to 14 while passing through the channel 20. Alternatively, the injected sample passes through the channel 20, for example, pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH 10, pH 11, A salt is coated on the surface of the microbeads 100 to be adjusted to the desired pH of pH 12, pH 13, ie to the pH of the microbeads 100.

The microbeads 100 are incubated in a buffer (buffer) to adjust to a desired pH, and then the solvent components are volatilized at 50 ° C. and N ₂ atmosphere for each desired pH concentration. Can be prepared. Buffers can be prepared, including, by way of example, the reagents in [Table 1]. More specifically, according to the following Henderson-Hasselbach equation to adjust the ratio of the base and the acid can be prepared a desired pH buffer solution.

Here, K _a is an acid dissociation constant, [HA] is an acid concentration, [A-] is a conjugate base, and [H +] is a hydrogen ion concentration.

In other words, the buffer solution above pH 0 and below pH 14 or corresponding to pH 1 to pH 13 may be a combination of weak / strong acid, acid / base, acid / pair base, weak / strong base, salt / base reagent combination. It can be prepared through. In general, acid / base regent combinations and their composition ratios allow for the preparation of buffers with varying pH values, but, for example, for weak acid / strong acid regent combinations, acidic buffer solutions of pH 2 to pH 4 may be used. It can be used predominantly for the preparation, and for combinations of weak and strong base regents, it can be used predominantly for preparing basic buffer solutions of pH 9 to pH 11.

In addition, the micro beads 100 are, for example, silica-based materials, and all components of the material in the pH buffer may be adsorbed onto the micro beads 100. At this time, the pH of the microbeads is determined according to the composition ratio of all the components of the substance except the solvent component in each pH buffer (for example, the composition ratio of the substance of Table 1 below). When the neutral sample passes through the microbeads 100 prepared as described above, salts adsorbed on the microbeads 100 are eluted to change the pH of the sample, thereby controlling the sample having a desired pH.

Examples of Reagents That Can Prepare a pH Buffer Hydrochloric acid / Potassium chloride Glycine / Hydrochloric acid Potassium hydrogen phthalate / hydrochloric acid Citric acid / Sodium citrate Sodium acetate / Acetic acid Potassium hydrogen phthalate / Sodium hydroxide Disodium hydrogen phthalate / Sodium dihydrogen orthophosphate Dipotassium hydrogen phthalate / Potassium dihydrogen orthophosphate Potassium dihydrogen orthophosphate / Sodium hydroxide Barbitone sodium / hydrochloric acid Tris (hydroxylmethyl) aminomethane / Hydrochloric acid Sodium tetraborate / Hydrochloric acid Glycine / Sodium hydroxide Sodium carbonate / Sodium hydrogen carbonate Sodium tetraborate / Sodium hydroxide Sodium bicarbonate / Sodium hydroxide Sodium hydrogen orthophosphate / Sodium hydroxide Potassium chloride / Sodium hydroxide

In Table 1, in the case of Potassium hydrogen phthalate / Hydrochloric acid, Potassium hydrogen phthalate is used as a weak acid, Hydrochloric acid is used as a strong acid, the concentration of hydrogen ions and paired bases from Potassium hydrogen phthalate depending on the amount of the hydrochloric acid, a strong acid The microbeads 100 are well coated with salts on the surface during the manufacturing process, but the microbeads 100 are present in the sample when the sample passes through the channel 20. In order that the salt coated on the eluted well, for example, the silica-based material may have a Si-OH group. In addition, the micro beads 100 may be any surface as long as all the components of the material in the pH buffer can be adsorbed well, and may have a smooth surface or a porous structure. In the case of a porous structure, the large surface area may facilitate the pH control of a larger volume of the sample. In addition, the diameter of the micro beads 100 may be 150 μm to 210 μm, for example.

In addition, the micro beads 100 may be filled in the channel 20 with, for example, several tens to several hundred mg, and the sample injected into the inlet 10 may use several ml.

In addition, the shape of the channel 20, as shown in the present invention, but may be of a hollow shape, for example, a cylindrical shape, but is not limited to this, is located between the injection port 10 and the outlet 30 As long as the micro beads 100 are filled therein, various modifications and changes are possible depending on the environment in which the present invention is implemented.

On the other hand, if the channel 20 is made of a cylindrical, for example, the height of the channel 20 may be 7.5cm and the diameter may be 1cm, likewise, the size and volume of the channel 20 is not limited thereto, and the present invention Various modifications and changes are possible depending on the implemented environment.

The sample injected into the apparatus 1 including the microbeads capable of pH adjustment of the sample may be neutral, for example, having a value of pH 6.8 to 7.9.

A frit 21 may be further included between the outlet 30 and the region in which the microbeads 100 are filled in the channel 20. Therefore, the sample passing through the micro beads 100 is discharged to the outlet 30, the micro beads 100 may be prevented from exiting to the outlet (30). The frit 21 may be, for example, glass wool.

The sample injected into the injection hole 100 may pass through the micro beads 100 filled in the channel 20, pass through the frit 21, and be discharged to the outlet 30. The sample discharged to the outlet 30 may be adjusted to a desired pH, and preferably may have the same pH as the pH of the micro beads 100.

FIG. 2 is a schematic diagram of a device 1 ′ comprising microbeads capable of multiple pH adjustment of a sample, with some modification of the device of FIG. 1. Device 1 ′ comprising microbeads capable of multiple pH adjustment of the sample comprises a plurality of channels 20 _i . Where i is a natural number from 1 to n. Each of the plurality of channels 20 _i may have micro beads 100 _i having different pHs from each other.

In addition, the apparatus 1 ′ including the microbeads capable of adjusting the pH of the sample may be connected to each of the plurality of channels 20 _i and a plurality of inlets 10 _i and a plurality of channels through which the sample may be injected. Each of them comprises a plurality of outlets (30 _i ) is discharged of the sample is pH adjusted. More specifically, each channel 20 _{i is} connected to each inlet 10 _i and each outlet 30 _i . The injection hole 10 _i of each channel 20 _i is connected to the main passage part 15 together with the main injection part 5, and the main passage part 15 has an aliquot structure. Thus, the sample introduced into one of the main injection unit 5 through the main passage part 15, is injected into the injection port (10 _i) of each channel (20 _i) filled in each of the channels (20 _i) Passed through the microbeads (100 _i ) to each outlet (30 _i ).

In addition, each injection port (10 _i) may further comprise a closing valve (not shown). Accordingly, it can be adjusted by the on-off valve so that the sample can pass only to the channel that can be adjusted to the desired pH of the plurality of channels.

In the case of Figure 2, the plurality of channels (20 _i ) is a sample injected into the main inlet 5, for example, pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7 , pH 8, pH 9, pH 10, pH 11, pH 12, pH 13 so that it can be discharged. The micro beads 100 _i respectively filled in the plurality of channels 20 _i are respectively pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH 10, pH It has a value of 11, pH 12, pH 13, may be prepared with the buffer of the above-mentioned [Table 1].

In the case of the apparatus 1 ′ including the microbeads capable of adjusting the pH of the sample as shown in FIG. 2, the sample injected into one main injection part 5 simultaneously passes through each of the plurality of channels 20 _i . The pH can be adjusted to the desired pH. Accordingly, there is an advantage that can be adjusted more efficiently while adjusting the pH of the sample in multiple.

FIG. 3 is a schematic diagram of an apparatus 1 ″ comprising microbeads capable of multiple pH adjustment of a sample, embodying the apparatus of FIG. 2 in one embodiment.

The microbeads 100 _i respectively filled in the plurality of channels 20 _i are for example pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH It has a value of 10, pH 11, pH 12, pH 13, may be prepared with the buffer of the above-mentioned [Table 1]. Where i is a natural number from 1 to n. The configuration overlapping with that of FIG. 2 will not be described.

The device 1 ″ including the microbeads capable of adjusting the pH of the sample may be more easily injected into each of the plurality of channels 20 _i simultaneously with the sample injected into one main injection section 5. The main injection part 5 may further include an injection member 40. In addition, the injection member 40 may further include a piston 41, and may further include a volume controller 42. One end of the injection member 40 may be connected to the main injection unit 5, and the piston 41 may be positioned at the other end of the injection member 40. In operation, for example, the piston 41 is removed and the sample is injected into the injection member 40, and then the piston 41 is mounted into the injection member 40 to push the piston 41 to the channel 20 _i. It may be to move the sample toward). The volume controller 42 may, for example, be in the form of a multichannel pipette, and after being set to a predetermined volume, the pipette may be operated to fill each channel connected to the pipette with the same volume of sample.

Experimental Example

[Table 2] shows the pH change of the sample after passing the sample of pH 7.7 through the channel 20 filled with the micro beads (100). The amount of the injected sample is 2 ml, and the microbeads 100 are filled in the channel 20 at 100 mg. The pH values in Table 2 are the results measured with S220 SevenCompact ^™ pH / Ion (METTLER TOLEDO).

PH of beads PH of sample before passing beads Beads manufacturing temperature Room temperature 50 ℃ 70 ℃ 90 ℃ PH of sample after passing beads Deviation PH of sample after passing beads Deviation PH of sample after passing beads Deviation PH of sample after passing beads Deviation One 7.7 3.7 -2.7 2.5 -1.5 7.1 -6.1 8.2 -7.2 2 7.7 1.1 0.9 2.6 -0.6 2.0 0.0 2.9 -0.9 3 7.7 2.1 0.9 3.5 -0.5 2.7 0.3 3.0 0.0 4 7.7 3.8 0.2 4.7 -0.7 5.0 -1.0 3.1 0.9 5 7.7 6.8 -1.8 5.5 -0.5 5.5 -0.5 6.9 -1.9 6 7.7 6.0 0.0 5.9 0.1 6.0 0.0 6.0 0.0 7 7.7 7.1 -0.1 7.0 0.0 6.9 0.1 7.1 -0.1 8 7.7 8.2 -0.2 7.9 0.1 8.1 -0.1 8.2 -0.2 9 7.7 8.0 1.0 8.9 0.1 8.9 0.1 9.0 0.0 10 7.7 8.9 1.1 9.9 0.1 8.3 1.7 9.4 0.6 11 7.7 9.7 1.3 10.7 0.3 - - 12 7.7 10.8 1.2 10.8 1.2 11.3 0.7 11.4 0.6 13 7.7 11.7 1.3 11.7 1.3 11.4 1.6 12.4 0.6

4 graphically shows [Table 2]. According to the results of the microbeads 100 manufacturing experiment according to the temperature, when the microbeads 100 were manufactured at 50 ° C., the pH variation between the sample and the microbeads 100 after passing through the microbeads 100 was smallest. It can be seen that. As the pH of the microbeads 100 increases, the condition in which the correlation of the pH of the sample after the microbeads 100 passes is constant may be set as an optimal condition. Referring to the experimental results of [Table 2], the optimum temperature condition of the microbeads 100 is 50 ℃. On the other hand, in the case of pH 1 and 13, Reagents are volatilized when the microbeads 100 are manufactured to generate a pH deviation.

It will be appreciated that the technical configuration of the present invention described above may be implemented in other specific forms by those skilled in the art without changing the technical spirit or essential features of the present invention. Therefore, it is to be understood that the embodiments described above are exemplary in all respects and not restrictive. In addition, the scope of the present invention is shown by the claims below, rather than the above detailed description. In addition, all changes or modifications derived from the meaning and scope of the claims and equivalent concepts should be construed as being included in the scope of the present invention.

1: A device containing microbeads capable of pH adjustment of a sample
10: inlet
20: channel
21: frit
30: exit
1 ', 1 ”: A device containing microbeads capable of adjusting the pH of a sample
10 _i : inlet
20 _i : Channel
30 _i : exit
100 _i : Micro Beads
5: main injection part
15: main passage
40: injection member
41: piston
42: volume controller

Claims (10)

A channel filled with microbeads capable of pH adjustment of the sample;
An inlet through which the sample can be injected into the channel; And
And an outlet through which the discharge of the sample with pH adjustment is made in the channel,
The injected sample is characterized in that the pH of the microbeads in the channel is adjusted, the device comprising a microbeads of the pH control of the sample. The method of claim 1,
Apparatus comprising a micro-bead that can adjust the pH of the sample, the salt is coated on the surface of the microbeads so that the injected sample can be adjusted to the desired pH in the channel. The method of claim 1,
The injected sample is adjusted to any one of pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH 10, pH 11, pH 12, pH 13 , Microbeads capable of pH adjustment of the sample. The method of claim 1,
And a frit between the outlet and the region filled with the microbeads in the channel,
The sample passing through the micro-beads through the frit is discharged to the outlet, wherein the micro-beads do not escape to the outlet, the apparatus comprising a micro-bead capable of pH adjustment of the sample. The method of claim 1,
The channel is composed of a plurality,
Each of the plurality of channels is filled with micro beads having a different pH from each other,
Wherein each channel comprises microbeads capable of pH adjustment of the sample connected to respective inlets and respective outlets. The method of claim 5, wherein
A main injection unit into which a sample is injected; And
Further comprising a main passage connecting the main injection portion and each injection hole, the main passage portion having an aliquot structure;
The sample injected into the main injection portion is distributed to the respective channels through the main passage portion, each of which is adjusted to the pH of the micro-beads filled in the respective channels, the pH of the sample includes a micro-bead that can be adjusted Device. The method of claim 5, wherein
Each channel comprises microbeads having pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH 10, pH 11, pH 12, and pH 13, respectively. To, the device comprising a micro-beads capable of adjusting the pH of the sample. The method of claim 6,
Further comprising an injection member connected to the main injection portion,
The injection member further comprises a piston,
One end of the injection member is connected to the main injection portion, a piston is located at the other end of the injection member,
And a microbead capable of pH adjustment of the sample, by which the piston can be pushed into the injection member to allow the injected sample to move into the respective channels. The method of claim 1,
The sample injected into the inlet is neutral, the device comprising a micro-bead capable of adjusting the pH of the sample. A microbead for use in an apparatus comprising microbeads capable of pH adjustment of a sample according to any one of claims 1 to 9.

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