KR20200025390A - A disc type device incluing microbeads capable of controlling pH of a sample - Google Patents

Abstract

The present invention relates to a disc type device capable of adjusting the pH of a sample and, more specifically, provides a disc type device comprising microbeads capable of adjusting the pH of a sample to a predetermined value. Also, provided is the disc type device which comprises, in each of a plurality of channels, microbeads enabling adjustment to different pH values, and thus enables adjustment to a plurality of different pH values simultaneously upon injection of the sample.

Description

A disc type device incluing microbeads capable of controlling pH of a sample}

The present invention relates to a disk-type device capable of adjusting the pH of a sample, and more particularly, to a disk-type device including microbeads capable of adjusting the sample to a desired pH, and also to a different pH for each of the plurality of channels. Disc type devices, including microbeads, which can be adjusted to a plurality of different pH simultaneously by injection of a sample.

In general, in order to adjust the pH, the pH value obtained by adding an acid or base solution to a sample is measured by a pH meter or a pH paper sensor, and a process of optimizing for reaching a desired pH value is performed.

In the case of such a conventional automatic pH control disk type device, by continuously adding acid or base to observe the pH of the sample in real time through the electrochemical disk type device using a valve disk type device, in this process It is costly and time consuming and therefore uneconomical.

On the other hand, for the conventional pH adjustment, a series of processes that measure the pH value obtained by adding an acid or base solution to a sample with a pH meter or a pH paper sensor and perform until a desired pH value is achieved are labor intensive and long time. The disadvantage is that it takes a lot of money. Accordingly, an object of the present invention is to provide an automated disk-type device that can more easily and quickly adjust a sample to multiple pHs in order to solve a problem in a series of optimization processes for pH adjustment.

Disc-type device comprising a micro-beads capable of adjusting the pH of the sample according to an embodiment of the present invention:

A main injection unit into which a sample is injected;

A plurality of channels filled with micro beads capable of adjusting the pH of the sample;

A plurality of injection holes through which the sample from the main injection portion may be injected into each of the plurality of channels; And

Each of the plurality of channels includes a plurality of outlets through which the discharge of the sample is pH adjusted,

Each pair of the plurality of inlets, the plurality of channels, and the plurality of outlets are radially disposed on a rotating disk,

The injected sample may be characterized in that each is adjusted to the pH of the micro beads filled in each channel.

In addition, in the disk-type device comprising a micro-beads capable of adjusting the pH of the sample according to an embodiment of the present invention, the salt (on the surface of the micro-beads so that the injected sample can be adjusted to the desired pH in the channel) salt) may be coated.

Disc-type device comprising a micro-beads capable of adjusting the pH of the sample according to an embodiment of the present invention:

A main passage part connected to the main injection part; And

An aliquot passage portion extending radially from said main passage portion to said respective inlets;

The main injection unit may include an opening through which the sample is injected and a sample reservoir for storing the sample before the injected sample is distributed to each aliquot passage part.

In addition, in the disk-type device including a micro-bead capable of adjusting the pH of the sample according to an embodiment of the present invention, the shape of the sample reservoir and the main passage portion may be spiral.

In addition, in the disk-type device including a micro-bead capable of adjusting the pH of the sample according to an embodiment of the present invention, each channel is pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, microbeads having pH 7, pH 8, pH 9, pH 10, pH 11, pH 12, and pH 13.

In addition, in the disk-type device comprising a micro-bead capable of adjusting the pH of the sample according to an embodiment of the present invention, by rotating the rotary disk at 1000 RPM for 20 seconds, each of the main passage through the main passage portion The sample is distributed to the aliquot passage part, and the rotating disk is rotated at 3000 RPM for 20 seconds to move from the respective aliquot passage part to the respective channel.

In addition, in the disk-type device including a micro-beads capable of adjusting the pH of the sample according to an embodiment of the present invention, the sample injected into the inlet may be neutral.

In addition, it is possible to provide a micro-beads used in the disk-type device including a micro-beads capable of pH adjustment of the sample according to an embodiment of the present invention.

According to the present invention, the microbeads can be adjusted to a desired pH more easily and quickly by including microbeads for pH adjustment in the channel, and each of the microbeads provided with a plurality of channels can be adjusted to a plurality of pH values. In case of a single sample injection, there is an advantage that can be adjusted to multiple desired pH at the same time.

In addition, the disk-type device comprising a micro-bead capable of adjusting the pH of the sample of the present invention is manufactured in the shape of a rotating disk, the sample injected through the control of the rotational force on the rotating disk into the beads filled channel having each pH You can pass it. These features can be integrated into sensing analysis systems through disk-based chemical reactions to improve detection sensitivity.

1 is a schematic diagram of a disk-type device including microbeads capable of adjusting the pH of a sample according to an embodiment of the present invention.
FIG. 2 is a schematic diagram of a disc shaped device including microbeads capable of multiple pH adjustment of a sample, with some variations of the disc shaped device of FIG.
FIG. 3 is a schematic diagram of a disc-shaped device comprising microbeads capable of multiple pH adjustment of a sample, embodying the disc-shaped device of FIG. 2 in one embodiment.
Figure 4 is a graph showing the results of the pH change experiment of the sample in the experimental example of the present invention.

Hereinafter, the disk-type device 1 including the microbeads capable of pH adjustment of a sample according to an embodiment of the present invention will be described in detail. The accompanying drawings show exemplary forms of the present invention, which are provided to explain the present invention in more detail, and the technical scope of the present invention is not limited thereto.

In addition, irrespective of the reference numerals, the same or corresponding components will be given the same reference numerals, and redundant description thereof will be omitted, and the size and shape of each illustrated member may be exaggerated or reduced for convenience of description. have.

1 is a schematic diagram of a disc-shaped device 1 including microbeads capable of pH adjustment of a sample according to an embodiment of the present invention. Disc-type device 1 including a micro-bead capable of adjusting the pH of the sample according to an embodiment of the present invention, the injection port 10 is a sample is injected, the channel 20, the pH of the injected sample is adjusted, pH And outlet 30 through which the discharge of the controlled sample is made.

The channel 20 includes the microbeads 100 to adjust the pH of the injected sample. A salt is coated on the surface of the micro beads 100 so that the injected sample can be adjusted to a desired pH of pH 0 to 14 while passing through the channel 20. Alternatively, the injected sample passes through the channel 20, for example, pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH 10, pH 11, A salt is coated on the surface of the microbeads 100 to be adjusted to a desired pH of pH 12 or pH 13.

The microbeads 100 are incubated in a buffer (buffer) to adjust to a desired pH, and then the solvent components are volatilized at 50 ° C. and N ₂ atmosphere for each desired pH concentration. Can be prepared. Buffers can be prepared, including, by way of example, the reagents in [Table 1]. More specifically, according to the following Henderson-Hasselbach equation to adjust the ratio of the base and the acid can be prepared a desired pH buffer solution.

Here, K _a is an acid dissociation constant, [HA] is an acid concentration, [A-] is a conjugate base, and [H +] is a hydrogen ion concentration.

In other words, the buffer solution above pH 0 and below pH 14 or corresponding to pH 1 to pH 13 may be a combination of weak / strong acid, acid / base, acid / pair base, weak / strong base, salt / base reagent combination. It can be prepared through. In general, acid / base regent combinations and their composition ratios allow for the preparation of buffers with varying pH values, but, for example, for weak acid / strong acid regent combinations, acidic buffer solutions of pH 2 to pH 4 may be used. It can be used predominantly for the preparation, and for combinations of weak and strong base regents, it can be used predominantly for preparing basic buffer solutions of pH 9 to pH 11.

In addition, the micro beads 100 are, for example, silica-based materials, and all components of the material in the pH buffer may be adsorbed onto the micro beads 100. At this time, the pH of the microbeads is determined according to the composition ratio of all the components of the substance except the solvent component in each pH buffer (for example, the composition ratio of the substance of Table 1 below). When the neutral sample passes through the microbeads 100 prepared as described above, salts adsorbed on the microbeads 100 are eluted to change the pH of the sample, thereby controlling the sample having a desired pH.

Examples of Reagents That Can Prepare a pH Buffer Hydrochloric acid / Potassium chloride Glycine / Hydrochloric acid Potassium hydrogen phthalate / hydrochloric acid Citric acid / Sodium citrate Sodium acetate / Acetic acid Potassium hydrogen phthalate / Sodium hydroxide Disodium hydrogen phthalate / Sodium dihydrogen orthophosphate Dipotassium hydrogen phthalate / Potassium dihydrogen orthophosphate Potassium dihydrogen orthophosphate / Sodium hydroxide Barbitone sodium / hydrochloric acid Tris (hydroxylmethyl) aminomethane / Hydrochloric acid Sodium tetraborate / Hydrochloric acid Glycine / Sodium hydroxide Sodium carbonate / Sodium hydrogen carbonate Sodium tetraborate / Sodium hydroxide Sodium bicarbonate / Sodium hydroxide Sodium hydrogen orthophosphate / Sodium hydroxide Potassium chloride / Sodium hydroxide

In Table 1, in the case of Potassium hydrogen phthalate / Hydrochloric acid, Potassium hydrogen phthalate is used as a weak acid, Hydrochloric acid is used as a strong acid, the concentration of hydrogen ions and paired bases from Potassium hydrogen phthalate depending on the amount of the hydrochloric acid, a strong acid The microbeads 100 are well coated with salts on the surface during the manufacturing process, but the microbeads 100 are present in the sample when the sample passes through the channel 20. In order that the salt coated on the eluted well, for example, the silica-based material may have a Si-OH group. In addition, the micro beads 100 may be any surface as long as all the components of the material in the pH buffer can be adsorbed well, and may have a smooth surface or a porous structure. In the case of a porous structure, the large surface area may facilitate the pH control of a larger volume of the sample. In addition, the diameter of the micro beads 100 may be 150 μm to 210 μm, for example.

In addition, the micro beads 100 may be filled in the channel 20 with, for example, several tens to several hundred mg, and the sample injected into the inlet 10 may use several ml.

In addition, the shape of the channel 20, as shown in the present invention, but may be of a hollow shape, for example, a cylindrical shape, but is not limited to this, is located between the injection port 10 and the outlet 30 As long as the micro beads 100 are filled therein, various modifications and changes are possible depending on the environment in which the present invention is implemented.

On the other hand, if the channel 20 is made of a cylindrical, for example, the height of the channel 20 may be 7.5cm and the diameter may be 1cm, likewise, the size and volume of the channel 20 is not limited thereto, and the present invention Various modifications and changes are possible depending on the implemented environment.

The sample injected into the disk-shaped device 1 including the microbeads capable of pH adjustment of the sample may be neutral, for example, having a value of pH 6.8 to 7.9.

A frit 21 may be further included between the outlet 30 and the region in which the microbeads 100 are filled in the channel 20. Therefore, the sample passing through the micro beads 100 is discharged to the outlet 30, the micro beads 100 may be prevented from exiting to the outlet (30). The frit 21 may be, for example, glass wool.

The sample injected into the injection hole 100 may pass through the micro beads 100 filled in the channel 20, pass through the frit 21, and be discharged to the outlet 30. The sample discharged to the outlet 30 may be adjusted to a desired pH, and preferably may have the same pH as the pH of the micro beads 100.

FIG. 2 is a schematic diagram of a disk-shaped device 1 ′ comprising microbeads capable of multiple pH adjustment of a sample, with some modification of the disk-shaped device of FIG. 1. Disc-shaped device 1 ′ comprising microbeads capable of multiple pH adjustment of a sample includes a plurality of channels 20 _i . Where i is a natural number from 1 to n. Each of the plurality of channels 20 _i may have micro beads 100 _i having different pHs from each other. The plurality of channels 20 _i may be, for example, two, four, six, eight, ten or twelve.

In addition, the disk-shaped device 1 ′ including microbeads capable of adjusting the pH of the sample is connected to each of the plurality of channels 20 _i and a plurality of inlets 10 _i and a plurality of inlets through which the sample can be injected. Each of the two channels includes a plurality of outlets (30 _i ) through which the discharge of the sample is adjusted pH. More specifically, each channel 20 _{i is} connected to each inlet 10 _i and each outlet 30 _i . The injection hole 10 _i of each channel 20 _i is connected to the main passage part 15 together with the main injection part 5, and the main passage part 15 has an aliquot structure. Thus, the sample introduced into one of the main injection unit 5 through the main passage part 15, is injected into the injection port (10 _i) of each channel (20 _i) filled in each of the channels (20 _i) Passed through the microbeads (100 _i ) to each outlet (30 _i ).

In addition, each injection port (10 _i) may further comprise a closing valve (not shown). Accordingly, it can be adjusted by the on-off valve so that the sample can pass only to the channel that can be adjusted to the desired pH of the plurality of channels.

In the case of Figure 2, the plurality of channels (20 _i ) is a sample injected into the main inlet 5, for example, pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7 , pH 8, pH 9, pH 10, pH 11, pH 12, pH 13 so that it can be discharged. The micro beads 100 _i respectively filled in the plurality of channels 20 _i are respectively pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH 10, pH It has a value of 11, pH 12, pH 13, may be prepared with the buffer of the above-mentioned [Table 1].

In the case of the disk-shaped device 1 'including the microbeads capable of adjusting the pH of the sample as shown in FIG. 2, the sample injected into one main injection section 5 simultaneously controls each of the plurality of channels 20 _i . Gina can be discharged by adjusting to the desired pH respectively. Accordingly, there is an advantage that can be adjusted more efficiently while adjusting the pH of the sample in multiple.

FIG. 3 is a schematic view of a disc shaped device 1 ″ comprising microbeads capable of multiple pH adjustment of a sample, embodying the disc shaped device of FIG. 2 in one embodiment.

The disk-shaped device 1 ″ comprising microbeads capable of adjusting the pH of the sample according to FIG. 3 has a rotating disk shape.

On the rotary disk 2 having the axis of rotation 3, a plurality of channels 20 _i as described above in FIG. 2 are arranged radially. Where i is a natural number from 1 to n. More specifically, the plurality of inlets 10 _i connected to each of the plurality of channels 20 _i and a plurality of outlets 30 _i through which the discharge of the sample is adjusted pH in each of the plurality of channels is included. And each pair of the plurality of channels, the plurality of inlets, and the plurality of outlets are radially disposed on a rotating disk.

The microbeads 100 _i respectively filled in the plurality of channels 20 _i are for example pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH It has a value of 10, pH 11, pH 12, pH 13, may be prepared with the buffer of the above-mentioned [Table 1].

The main injection part 5 is arranged near the rotation shaft 3, and in order of the main injection part 5, the main passage part 15, the injection port 10 _i , the channel 20 _i , and the outlet 30 _i . It is arranged in the edge direction on the rotation axis 3 of the rotary disk 2. The main injection unit 5 includes an opening 5a into which a sample is injected. It also includes a sample reservoir 5b that stores the sample before the injected sample is dispensed into each channel 20 _i . The sample reservoir 5b may have a shape surrounding the rotation shaft 3. The main passage part 15 is connected to the end of the sample reservoir 5b. The main passage part 15 may also have a shape surrounding the rotation shaft 3. The shape in which the sample reservoir 5b and the main passage part 15 are coupled may be spiral.

The main passage portion 15 is connected to the plurality of aliquot passage portions 16 _i . Each of the aliquot passage portions 16 _i is radially extended from the main passage portion 15 to each channel 20 _i . Samples of the main passage portion 15 are to be dispensed by the volume of each aliquot passage portion 16 _i . Accordingly, the sample injected into one main injection section 5 passes through the main passage section 15 and is distributed to the aliquot passage section 16 _i . A sample distribution in Ally quart passage portion (16 _i) has been injected into the injection port (10 _i) of each channel (20 _i), the micro-beads (see 100 _i, 2 degrees) filled in each of the channels (20 _i) Pass through to each exit 30 _i . Injection port (10 _i), the on-off valve a may further include, on-off valve (11 _i) (11 _i), for example a capillary valve (capillary valve) may be, further comprising a vent hole (11a _i) can do. As will be described later, the sample passes through the capillary valve, for example, upon applying a rotational force of 3000 RPM. The outlet 30 _i may have a structure that can accommodate the pH adjusted sample while passing through the channel 20 _i . In such a case, the pH-adjusted sample can be recovered later by a micro pipette through the vent hole 31 _i if necessary. The outlet 30 _i may further include a vent hole 31 _i . By providing the vent hole 31 _i , the air filled in the channel 20 _i is drawn out so that the fluid can move to the outlet 30 _i by the rotational force.

Regarding the operation of the disk-shaped device 1 ″ including the microbeads capable of adjusting the pH of the sample, for example, the sample is injected into the main inlet 5 and the rotating disk 2 is rotated at 1000 RPM. When rotated for a second, the sample moves from the main injection section 5 through the main passage section 15 to the aliquot passage section 16 _i to be distributed. Next, when 20 seconds rotating the rotary disk 2 to 3000RPM, distributed to Alley quart passage portion (16 _i), the sample is moved to each of the channels (20 _i) filled in each of the channels (20 _i) microbeads Through the sample is adjusted to the desired pH is moved to the outlet (30 _i ).

Experimental Example

 [Table 2] shows the pH change of the sample after passing the sample of pH 7.7 through the channel 20 filled with the micro beads (100). The amount of sample injected is 2 ml, when the microbeads 100 are filled in the channel 20 in a volume of 100 mg.

The pH values in Table 2 are the results measured with S220 SevenCompact ^™ pH / Ion (METTLER TOLEDO).

PH of beads PH of sample before passing beads Beads manufacturing temperature Room temperature 50 ℃ 70 ℃ 90 ℃ PH of sample after passing beads Deviation PH of sample after passing beads Deviation PH of sample after passing beads Deviation PH of sample after passing beads Deviation One 7.7 3.7 -2.7 2.5 -1.5 7.1 -6.1 8.2 -7.2 2 7.7 1.1 0.9 2.6 -0.6 2.0 0.0 2.9 -0.9 3 7.7 2.1 0.9 3.5 -0.5 2.7 0.3 3.0 0.0 4 7.7 3.8 0.2 4.7 -0.7 5.0 -1.0 3.1 0.9 5 7.7 6.8 -1.8 5.5 -0.5 5.5 -0.5 6.9 -1.9 6 7.7 6.0 0.0 5.9 0.1 6.0 0.0 6.0 0.0 7 7.7 7.1 -0.1 7.0 0.0 6.9 0.1 7.1 -0.1 8 7.7 8.2 -0.2 7.9 0.1 8.1 -0.1 8.2 -0.2 9 7.7 8.0 1.0 8.9 0.1 8.9 0.1 9.0 0.0 10 7.7 8.9 1.1 9.9 0.1 8.3 1.7 9.4 0.6 11 7.7 9.7 1.3 10.7 0.3 - - 12 7.7 10.8 1.2 10.8 1.2 11.3 0.7 11.4 0.6 13 7.7 11.7 1.3 11.7 1.3 11.4 1.6 12.4 0.6

4 graphically shows [Table 2]. According to the results of the microbeads 100 manufacturing experiment according to the temperature, when the microbeads 100 were manufactured at 50 ° C., the pH variation between the sample and the microbeads 100 after passing through the microbeads 100 was smallest. It can be seen that. As the pH of the microbeads 100 increases, the condition in which the correlation of the pH of the sample after the microbeads 100 passes is constant may be set as an optimal condition. Referring to the experimental results of [Table 2], the optimum temperature condition of the microbeads 100 is 50 ℃. On the other hand, in the case of pH 1 and 13, Reagents are volatilized when the microbeads 100 are manufactured to generate a pH deviation.

It will be appreciated that the technical configuration of the present invention described above may be implemented in other specific forms by those skilled in the art without changing the technical spirit or essential features of the present invention. Therefore, it is to be understood that the embodiments described above are exemplary in all respects and not restrictive. In addition, the scope of the present invention is shown by the claims below, rather than the above detailed description. In addition, all changes or modifications derived from the meaning and scope of the claims and equivalent concepts should be construed as being included in the scope of the present invention.

1: Disc type device including microbeads for pH adjustment of a sample
10: inlet
20: channel
21: frit
30: exit
1 ', 1 ”: Disc-shaped device with microbeads for multiple pH adjustment of samples
5: main injection part
15: main passage
10 _i : inlet
11 _i : on-off valve
20 _i : Channel
30 _i : exit
31 _i : Vent hole
100 _i : Micro Beads

Claims (8)

A main injection unit into which a sample is injected;
A plurality of channels filled with micro beads capable of adjusting the pH of the sample;
A plurality of injection holes through which the sample from the main injection portion may be injected into each of the plurality of channels; And
Each of the plurality of channels includes a plurality of outlets through which the discharge of the sample is pH adjusted,
Each pair of the plurality of inlets, the plurality of channels, and the plurality of outlets are radially disposed on a rotating disk,
The injected sample is a disk-type device comprising a micro-bead capable of adjusting the pH of the sample, characterized in that each of the adjusted to the pH of the micro beads filled in each channel. The method of claim 1,
Salt-coated on the surface of the micro-beads so that the injected sample can be adjusted to the desired pH in the channel, the disk-type device comprising a micro-beads can adjust the pH of the sample. The method of claim 1,
A main passage part connected to the main injection part;
An aliquot passage portion extending radially from said main passage portion to said respective inlets;
The main injection unit includes a micro-bead capable of adjusting the pH of the sample including an opening into which the sample is injected and a sample reservoir for storing the sample before the injected sample is distributed to each aliquot passage portion. The method of claim 3, wherein
Disc-shaped device comprising a micro-bead capable of pH adjustment of the sample is a spiral shape of the sample reservoir and the main passage portion coupled. The method of claim 1,
Each channel comprises microbeads having pH 1, pH 2, pH 3, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH 10, pH 11, pH 12, and pH 13, respectively. A disk-type device comprising a micro-beads that can adjust the pH of the sample. The method of claim 1,
Rotating the rotating disk for 20 seconds at 1000 RPM, the sample is distributed from the main injection portion through the main passage portion to each alliance passage portion,
Rotating the rotating disk for 20 seconds at 3000RPM, the disk-type device comprising a micro-bead capable of pH adjustment of the sample to be moved to the respective channels in each aliquot passage. The method of claim 1,
The sample injected into the inlet is neutral, the disk-type device comprising a micro-bead capable of adjusting the pH of the sample. A microbead for use in a disc-shaped device comprising microbeads capable of pH adjustment of a sample according to any one of claims 1 to 7.

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