KR20200009706A - Powder of lactic acid bacteria and method for preparing powder of lactic acid bacteria having the ability to degrade gluten with polysaccharide produced by Tremella fuciformis and β-glucan - Google Patents
Powder of lactic acid bacteria and method for preparing powder of lactic acid bacteria having the ability to degrade gluten with polysaccharide produced by Tremella fuciformis and β-glucan Download PDFInfo
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Abstract
Description
본 발명은 백목이버섯 균사체 유래 다당체 및 베타글루칸을 이용한 글루텐 분해 유산균의 이중 코팅을 통한 유산균의 생존력이 향상된 유산균 분말 및 그의 제조방법에 관한 것으로, 더욱 구체적으로 글루텐 분해용 유산균을 폴리머로 이중 코팅한 유산균 분말 및 그의 제조방법에 관한 것이다.The present invention relates to a powder of lactic acid bacteria improved viability of lactic acid bacteria through double coating of gluten degrading lactic acid bacteria using polysaccharide and beta glucan derived from Bacillus mycelium mycelium, and more specifically, to double-coated lactic acid bacteria for gluten decomposition with polymer It relates to a lactic acid bacteria powder and a method for producing the same.
유산균(lactic acid bacteria)은 인류가 가장 오랫동안 광범위하게 활용하고 있는 미생물로서, 발효유 제조, 건강 및 기능성 식품, 정장제, 의약품 및 생균제제로 활용되고 있다. 유산균은 동물의 생장 촉진, 사료 이용효율 증가, 질병에 대한 저항력 증대, 유해세균의 증식 억제, 폐사율 감소 및 부패 독성물질의 생성억제 등의 특징이 있으며, 또한 인간의 장내에 기생하면서 장의 연동운동을 활발하게 유지시켜 장내 이상발효를 억제하고, 장 기능을 활성화시켜 장 기능 이상을 개선시키는 동시에 섭생활동을 원활하게 유지시키는 역할을 한다.Lactic acid bacteria are the most widely used microorganisms for a long time, and are used in fermented milk production, health and functional foods, formal preparations, medicines and probiotic products. Lactic acid bacteria are characterized by promoting the growth of animals, increasing feed efficiency, increasing resistance to diseases, inhibiting the growth of harmful bacteria, reducing mortality and inhibiting the production of toxic toxins. It keeps active intestinal abnormal fermentation, and activates the intestinal function to improve the function of the intestinal function and at the same time plays a role of smoothly maintaining.
유산균은 주로 발효 산물을 이용하는 다른 산업용 미생물과는 달리 생균 자체를 이용하기 때문에 유통 기간 중 생존력의 유지는 매우 중요한 문제이다. 이와 같이 유산균은 효용과 가치 면에서 매우 유익한 균임에도 불구하고 상당히 불안정하여 장기간 보관하고 사용하는데 많은 제약이 따르며, 또한 이를 이용한 발효유제품 및 생균제제는 제조과정과 유통기간 중 산소나 산에 의하여 생존력이 크게 영향을 받는 것으로 알려져 있다[Molder etc al, Can. Inst. Food. Sci. Technol., 1990, 39, 900-907].Unlike other industrial microorganisms that mainly use fermentation products, lactic acid bacteria use viable bacteria themselves, so maintaining viability during distribution is a very important problem. As described above, lactic acid bacteria are highly unstable in terms of their utility and value, and therefore, are very unstable and therefore have many limitations in their storage and use for a long time. It is known to be greatly affected [Molder et al, Can. Inst. Food. Sci. Technol. , 1990, 39, 900-907.
유산균은 보통 액상으로 배양되어 이용되고 있으나, 편의상 또는 장기보존을 위해 이를 분말 형태로 제조하여 이용하기도 한다. 일반적으로 유산균은 동결 건조법 또는 분사 건조법에 의하여 분말 형태로 제조하며, 그 중 동결 건조법은 동결건조용 현탁용액에 미생물을 현탁시켜 동결시킨 후 감압 하에서 건조시키는 방법이다. 그러나, 유산균은 편혐기성이고 주위환경에 매우 민감하기 때문에 분말 유산균은 저온 및 상온에서 생존력이 저하되어 생균수가 감소하게 된다.Lactic acid bacteria are usually cultured and used in liquid form, but for convenience or long-term preservation may be used to prepare them in powder form. In general, lactic acid bacteria are prepared in powder form by freeze-drying or spray drying, wherein freeze-drying is a method of suspending microorganisms in a freeze-drying suspension solution to freeze and drying under reduced pressure. However, because lactic acid bacteria are anaerobic and very sensitive to the surrounding environment, the powdered lactic acid bacteria have a low viability at low temperature and room temperature, thereby reducing the number of viable bacteria.
구체적으로, Claude P. C. 등은 동결건조된 유산균의 안정성에 대한 저장온도 및 폴리머의 영향에 대해 연구 결과를 보고하였다[Food. Res. Int., 29(5-6), 555～562]. 상기 논문에서 젤라틴으로 코팅된 락토바실러스 람노서스(Lactobacillus rhamnosus)는 20 ℃에서 저장하여 6개월 후에는 약 1 %, 12개월 후에는 약 0.2 %의 생존율을 나타내었다.Specifically, Claude PC et al . Reported the results of the study on the effect of storage temperature and polymer on the stability of lyophilized lactic acid bacteria [ Food. Res. Int. , 29 (5-6), 555-562]. In this paper, gelatin coated Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) was stored at 20 ℃ and showed a survival rate of about 1% after 6 months, about 0.2% after 12 months.
Paul B. 등은 단당류(monosaccharide)를 이용한 락토바실러스 애시도필러스(Lactobacillus acidophilus)의 보존 및 안정성에 대해 보고하였다[Cryobiology, 2000, 41, 17～24]. 상기 논문에서 트레할로스(trehalose)로 코팅된 락토바실러스 애시도필러스는 상온(37℃)에서 35일 후 10 %의 생존율을 나타내었다.Paul B. et al. Reported the preservation and stability of Lactobacillus acidophilus using monosaccharides [ Cryobiology , 2000, 41, 17-24]. In this paper, trehalose-coated Lactobacillus ashidophilus showed a survival rate of 10% after 35 days at room temperature (37 ° C.).
Kim은 유산균의 캡슐 제조 방법을 보고하였다[J. Ind. Microbiol., 1998, 3, 253～257]. 상기 논문에서 카르복실메틸셀룰로오스(carboxylmethylcellulose)로 코팅된 락토바실러스 플란타룸은 32 ℃ 또는 37 ℃로 보관할 경우 1 주일만에 생존율이 1 %이하로 급격히 떨어짐을 알 수 있다.Kim reported a method for preparing capsules of lactic acid bacteria [ J. Ind. Microbiol ., 1998, 3, 253-257]. In the above paper, it can be seen that the Lactobacillus plantarum coated with carboxymethyl cellulose rapidly drops to less than 1% in one week when stored at 32 ° C or 37 ° C.
구선모 등은 알기내이트와 키토산을 사용한 마이크로캡슐화에 의한 락토바실러스 카제이(Lactobacillus casei) YIT 9018의 안정성 증진 방법이 보고되었다.[J. Microbiol. Biotechnol., 2001, 11(3), 376～383]. 상기 논문에서 락토바실러스 카제이 YIT 9018의 동결건조시 알기내이트와 키토산을 사용한 마이크로캡슐을 제조하는 방법을 제시하고 있다. 그러나 상기 방법에 의하면, 유산균이 상온에서 21일 경과 후에 최대로 약 30% 밖에 살지 못하는 등 생존력이 매우 낮기 때문에 분말 생균제로 사용하기에는 부적합하다.Kusunmo et al. Have reported a method for enhancing the stability of Lactobacillus casei YIT 9018 by microencapsulation using alginate and chitosan. [ J. Microbiol. Biotechnol ., 2001, 11 (3), 376-383]. In this paper, a method for preparing microcapsules using alginate and chitosan during lyophilization of Lactobacillus casei YIT 9018 is presented. However, according to the above method, lactic acid bacteria are not suitable for use as a powder probiotic because their viability is very low, such as living at a maximum of about 30% after 21 days at room temperature.
또한 K. Sultana 등은 알기내이트와 전분으로 박테리아 생균제의 캡슐화 및 장내 조건과 요거트에서의 생존능에 관한 방법을 보고하였다. 상기 논문에서 전분-알기내이트로 캡슐화된 유산균은 요거트 내에서 약 2개월 후 10 %의 생존율만을 나타내었다.K. Sultana et al. Also reported a method for encapsulating bacterial probiotics with intestinal and starch conditions and viability in yogurt. The lactic acid bacteria encapsulated with starch-alginate in this paper showed only 10% survival after about 2 months in yogurt.
상술한 바와 같이, 유산균의 장기보존을 위한 수단으로서 폴리머 코팅이나 알기내이트 포집 기술 등은 이미 알려져 있으나, 그 효과는 매우 크지 않은 것으로 보고되고 있다. As described above, as a means for long-term preservation of lactic acid bacteria, polymer coating, alginate collection technology and the like have already been known, but the effect is not reported to be very large.
미합중국 특허 제 4,399,2000호에는 배지에 N-아세틸-D-글루코사민(N-acetyl-D-glucosamine)을 첨가함으로써 플라스미드를 가진 락토바실러스 헬베티커스(Lactobacillus helveticus)를 안정화시키는 방법이 보고되었으나, 상기 방법은 건조시킨 결과를 제시하지 못했으며, 또한 N-아세틸-D-글루코사민이 고가라는 단점이 있다.U.S. Patent No. 4,399,2000 reports a method of stabilizing Lactobacillus helveticus with plasmids by adding N-acetyl-D-glucosamine to the medium. The method did not yield a result of drying, and also has the disadvantage that N-acetyl-D-glucosamine is expensive.
또한, 국내특허 제 1997-53312호는 젤라틴으로 코팅된 유산균을 사용하여 위산에 견디고 장내 도달율을 높인 식품류가 보고되었으나, 상기 코팅된 유산균은 인공 위액과 인공 장액에 대한 특이성의 차이가 없이 어떤 환경에서든 10 시간 이상 붕해되지 않음을 실험결과로 보여주고 있으며, 이는 코팅된 유산균이 위나 장의 환경에 따라 민감하게 반응하지 않는 일반적인 코팅에 불과하기 때문인 것으로 사료된다. 따라서 일반적인 코팅기술을 그대로 적용하는 것은 유산균의 생존율 향상 측면에서 그다지 만족스럽지 못하며 이에 따라 보다 효율적이고 경제적인 유산균 제제의 제조 방법이 요구되고 있다.In addition, Korean Patent No. 1997-53312 has reported a foodstuff that endured gastric acid and increased intestinal reach rate by using lactic acid bacteria coated with gelatin, but the coated lactic acid bacteria can be used in any environment without difference in specificity for artificial gastric juice and artificial intestinal fluid. Experimental results show that it does not disintegrate for more than 10 hours, which may be due to the fact that the coated lactic acid bacteria are only general coatings that are not sensitive to the stomach or intestinal environment. Therefore, applying the general coating technique as it is not very satisfactory in terms of improving the survival rate of lactic acid bacteria, and thus a more efficient and economical production method of lactic acid bacteria formulation is required.
이에, 본 발명자들은 상기한 문제점을 해결하고자 2 종의 다른 폴리머로 코팅된 유산균이 분말을 제조하였고, 분말 상태에서 유산균의 생존력 및 안정성을 향상시켜 저온 및 상온에서 유산균을 장기간 동안 보존할 수 있음을 알아내어 본 발명을 완성하였다.Thus, the present inventors have prepared a powder of lactic acid bacteria coated with two different polymers to solve the above problems, improve the viability and stability of the lactic acid bacteria in powder state can be preserved for a long time at low temperature and room temperature The present invention was completed by finding out.
본 발명자들은 글루텐 분해 유산균이 사람의 체내나 면 제품에 적용 시 우수한 생존율을 보이고 열에 대해서도 안정성을 보일 수 있는 이중 코팅 유산균을 개발하고자 하였다. 이에 백목이버섯 균사체 유래 다당체 및 베타글루칸을 이용한 글루텐 분해 유산균의 이중 코팅을 통해 생존율이 우수한 방법을 개발하여 본 발명의 완성하였다. The present inventors have attempted to develop a double-coated lactic acid bacteria that can show excellent survival rate and stability against heat when gluten-degrading lactic acid bacteria are applied to human body or cotton products. Accordingly, the method of excellent survival rate was developed by double coating of gluten-degraded lactic acid bacteria using polysaccharide and beta glucan derived from Bacillus mycelium mycelium, thereby completing the present invention.
따라서 본 발명의 목적은 백목이버섯 균사체 유래 다당체 및 베타글루칸을 이용하여 이중 코팅된 유산균을 제공하는데 있다.Therefore, it is an object of the present invention to provide a double-coated lactic acid bacteria using polysaccharides and beta glucan derived from Bacillus mycelia.
본 말명의 목적 및 이점은 발명의 상세한 설명 및 청구범위에 의해 보다 명확하게 제공한다.The objects and advantages of the present invention are more clearly provided by the description and the claims.
상기한 목적을 달성하기 위하여, 본 발명은 백목이버섯 균사체 유래 다당체 및 베타글루칸으로 코팅된 유산균 분말을 제공한다.In order to achieve the above object, the present invention provides a lactic acid bacteria powder coated with Bacillus mycelium mycelium-derived polysaccharide and beta glucan.
또한, 본 발명은 백목이버섯 균사체 유래 다당체 및 베타글루칸으로 이중 코팅된 유산균 분말의 제조방법을 제공한다.The present invention also provides a method for producing lactic acid bacteria powder double coated with Bacillus mycelium mycelium-derived polysaccharide and beta glucan.
이하 본 발명을 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명은 백목이버섯 균사체 유래 다당체 및 베타글루칸으로 코팅된 유산균 분말을 포함한다.The present invention includes lactic acid bacteria powder coated with mycelium mycelium mycelium and beta glucan.
본 발명의 유산균 분말은 유산균, 백목이버섯 균사체 유래 다당체 및 베타글루칸으로 구성되며, 이에 더하여 동결보호제를 더 포함할 수 있다.Lactic acid bacteria powder of the present invention is composed of lactic acid bacteria, Baekyi mushroom mycelium-derived polysaccharide and beta glucan, in addition, may further comprise a cryoprotectant.
유산균은 109～1010 CFU/㎖로 농축된 것으로, 스트렙토코커스(Streptococcus) 속, 락토코커스(Lactococcus) 속, 엔테로코커스(Enterococcus) 속, 락토바실러스(Lactobacillus) 속, 페디오코커스(Pediococcus) 속, 류코노스톡(Leuconostoc) 속, 비셀라(Weissella) 속 및 비피도박테리움(Bifidobacterium) 속의 균주로 이루어진 그룹 중 선택된 1 종 또는 2 종 이상으로 이루어져 있다.Lactobacillus is concentrated at 10 9 ~ 10 10 CFU / ㎖, Streptococcus genus, Lactococcus genus, Enterococcus genus, Lactobacillus genus, Pediococcus genus , flow consists of Pocono stock (Leuconostoc), a non-Cellar (Weissella) genus and Bifidobacterium (Bifidobacterium) in the strains alone or in combination of two or more selected from the group consisting of.
폴리머는 유산균 표면을 코팅하여 생존력을 증가시키는 역할을 하는 것으로, 본 발명에서 백목이버섯 균사체 유래 다당체 및 베타글루칸이 바람직하다. 도 2 및 도 3에서 보는 바와 같이, 백목이버섯 균사체 유래 다당체 및 베타글루칸을 첨가하여 유산균을 코팅한 경우, 4 ℃에서 2 개월 동안 보관한 결과, 유산균이 생존능을 유지하는데 가장 우수함을 알 수 있다.The polymer serves to increase the viability by coating the surface of lactic acid bacteria, polysaccharide and beta glucan derived from Bacillus mycelium mycelium in the present invention is preferred. As shown in Figures 2 and 3, when lactic acid bacteria mycelium-derived polysaccharide and beta-glucan is added to the coating of lactic acid bacteria, storage for 2 months at 4 ℃, it can be seen that the lactic acid bacteria are the best to maintain viability. .
본 발명은 추가로, 동결보호제를 더 포함할 수 있다. 동결보호제는 동결건조시 유산균의 생존력을 증대시키며, 본 발명에서는 탈지 분유 안정제, 솔비톨 또는 글리세롤이 사용된다.The present invention may further comprise a cryoprotectant. Lyoprotectants increase the viability of lactic acid bacteria during lyophilization, and skim milk powder stabilizer, sorbitol or glycerol is used in the present invention.
상기 탈지 분유 안정제는 탈지 분유, 수크로스, 아스코스빈산 및 글루탐산으로 이루어져 있다. 구체적으로, 유산균의 생존력을 고려하여, 탈지분유 8～12 중량%, 수크로스 1～4 중량%, 아스코스빈산 0.3～0.7 중량% 및 글루탐산 0.3～0.7 중량%가 바람직하다.The skim milk powder stabilizer consists of skim milk powder, sucrose, ascosvinic acid and glutamic acid. Specifically, in consideration of the viability of lactic acid bacteria, 8 to 12% by weight of skim milk powder, 1 to 4% by weight sucrose, 0.3 to 0.7% by weight ascorbic acid and 0.3 to 0.7% by weight glutamic acid.
본 발명은 폴리머로 코팅된 유산균을 금속 알기내이트 비드에 포집하는 단계를 포함하는 유산균 분말의 제조방법을 포함한다.The present invention includes a method for producing a lactic acid bacteria powder comprising the step of collecting the lactic acid bacteria coated with a polymer in the metal alginate beads.
구체적으로, 상기 유산균 분말의 제조방법이Specifically, the production method of the lactic acid bacteria powder
배양 농축된 유산균에 동결보호제를 혼합하는 단계(단계 1),Mixing the cryoprotectant to the culture concentrated lactic acid bacteria (step 1),
상기 혼합된 유산균에 폴리머 수용액을 첨가하여 유산균을 코팅하는 단계(단계 2),Coating the lactic acid bacteria by adding a polymer aqueous solution to the mixed lactic acid bacteria (step 2),
상기 코팅된 유산균을 건조하는 단계(단계3)으로 구성된다.It consists of a step (step 3) of drying the coated lactic acid bacteria.
상술한 바와 같이, 본 발명은 유산균을 백목이버섯 균사체 유래 다당체 및 베타글루칸으로 이중 코팅하고 이를 동결건조하여 분말제제화 함으로써 유산균의 생존력을 향상시킨 분말유산균을 제조하고 이를 장기간 보관할 수 있는 장점이 있다.As described above, the present invention has the advantage of producing a powder lactic acid bacteria to improve the viability of lactic acid bacteria by long-term storage of lactic acid bacteria by double coating with polysaccharides and beta glucan derived from Baekyi mushroom mycelium and lyophilized powder.
도 1은 유산균의 코팅 전과 후의 사진을 나타낸 그림이고,
도 2는 코팅되지 않은 유산균과 백목이버섯 균사체 유래 다당체 및 베타글루칸으로 코팅된 유산균 분말을 상온에서 보존 시 생존율을 나타낸 그림이고,
도 3 은 코팅되지 않은 유산균과 백목이버섯 균사체 유래 다당체 및 베타글루칸으로 코팅된 유산균 분말을 저온(4)에서 보존 시 생존율을 나타낸 그림이다.1 is a picture showing a photo before and after the coating of lactic acid bacteria,
Figure 2 is a diagram showing the survival rate when preserving the lactic acid bacteria and the lactic acid bacteria mycelium-derived polysaccharide and beta glucan-coated lactic acid powder at room temperature,
Figure 3 is a figure showing the survival rate when preserving the lactic acid bacteria and lactic acid bacteria mycelium-derived polysaccharide and beta glucan-coated lactic acid powder at low temperature (4).
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. These Examples are only for more specifically describing the present invention, and according to the gist of the present invention, the scope of the present invention is not limited to these Examples. It will be apparent to those of ordinary skill in the art.
유산균 분말의 제조Preparation of Lactic Acid Bacteria Powder
본 발명에 이용된 유산균은 락토바실러스 플란타룸(수탁번호 : KCTC 13532BP)을 포함한 스트렙토코커스(Streptococcus) 속, 락토코커스(Lactococcus) 속, 엔테로코커스(Enterococcus) 속, 락토바실러스(Lactobacillus) 속, 페디오코커스(Pediococcus) 속, 류코노스톡(Leuconostoc) 속, 비셀라(Weissella) 속 및 비피도박테리움(Bifidobacterium) 속을 사용하였고, 상기 유산균은 하기 표 1에 나타낸 엠알에스(MRS) 액체 배지에 접종하여 각각 37 ℃에서 12시간 동안 정치 배양하여 유산균의 농도가 109～1010 CFU/㎖가 되도록 하였다. 배양된 유산균은 6,000 알피엠(rpm)으로 10분간 원심 분리하여 유산균을 수집하였고, 이를 1/10 양의 멸균수나 생리식염수(0.8% 염화나트륨)로 두 번 세척하였고, 유산균의 농도가 1010 CFU/㎖가 되도록 탈지분유 안정제를 첨가한 후 교반기를 이용하여 균일하게 교반하였다.The lactic acid bacteria used in the present invention are Streptococcus genus including Lactobacillus plantarum (Accession Number: KCTC 13532BP), Lactococcus genus, Enterococcus genus, Lactobacillus genus, Pedy O Rhodococcus (Pediococcus), a flow Pocono stock (Leuconostoc), a non-Cellar (Weissella) genus, and was used in Bifidobacterium (Bifidobacterium), the lactic acid bacteria in the emal S (MRS) broth shown in Table I. Inoculated and incubated for 12 hours at 37 ℃ each so that the concentration of lactic acid bacteria was 10 9 ~ 10 10 CFU / ㎖. The cultured lactic acid bacteria were centrifuged for 10 minutes at 6,000 AlpM (rpm) to collect lactic acid bacteria, which were washed twice with 1/10 amount of sterile water or saline (0.8% sodium chloride), and the concentration of lactic acid bacteria was 10 10 CFU / ml. After adding the skim milk powder stabilizer so that it was uniformly stirred using a stirrer.
교반한 유산균에 백목이버섯 균사체 유래 다당체로 1 차 코팅하고, 베타글루칸으로 2 차 코팅한다.The stirred lactic acid bacteria were firstly coated with Bacillus mycelium mycelium-derived polysaccharide and secondly with beta glucan.
백목이버섯 균사체 유래 다당체 및 베타글루칸으로 이중 코팅된 유산균의 생존력 측정Viability Measurement of Lactic Acid Bacteria Double-Coated with Polysaccharides and Beta-glucans
상기 실시예 1에서 제조된 유산균을 영하 70 ℃의 냉동고에서 1～3 시간 동안 동결시킨 후, 동결건조기를 이용하여 압력은 10 mmHg 및 응집온도 영하 50℃를 유지시켜 감압 상태에서 10～16시간 동안 동결건조시켰다. 얻어진 유산균을 멸균된 유리병에 넣고 각각 4 ℃와 30 ℃의 항온기에서 보관하면서 2～3 주 간격으로 500 ㎎씩 채취하였다. 유산균 분말을 10 % 수크로오스 용액에서 2 시간 동안 현탁한 후 멸균수를 이용하여 연속 희석한 후 엠알에스 평판배지에 도말하여 30 ℃에서 16～20 시간 배양하여 나타난 집락수(Colony Forming Unit, CFU)를 비교하고, 유산균의 생존력을 표시하였다.After freezing the lactic acid bacteria prepared in Example 1 in a freezer at minus 70 ℃ for 1 to 3 hours, using a freeze dryer for 10 to 16 hours under reduced pressure by maintaining a pressure of 10 mmHg and a coagulation temperature below 50 ℃ Lyophilized. The obtained lactic acid bacteria were placed in sterile glass bottles and collected at 500 mg at intervals of 2 to 3 weeks while being kept in a thermostat at 4 ° C. and 30 ° C., respectively. The lactic acid bacteria powder was suspended in a 10% sucrose solution for 2 hours, and then serially diluted with sterile water, and then plated on a plate of MLS and incubated at 30 ° C. for 16 to 20 hours to obtain colony forming unit (CFU). The viability of the lactic acid bacteria was compared and displayed.
도 2 와 3에 나타낸 바와 같이 상온 및 저온(4℃)에서 1 개월 간 보존한 경우, 본 발명에서 사용한 유산균의 경우, 코팅을 하지 않은 유산균에 비하여 우수한 생ㅈ본력을 나타냄을 확인함으로써 본 말병을 완성하였다. As shown in FIGS. 2 and 3, when stored at room temperature and low temperature (4 ° C.) for 1 month, the lactic acid bacteria used in the present invention exhibited excellent life force as compared to the non-coated lactic acid bacteria. Completed.
Claims (3)
Double coated lactobacillus powder of gluten degrading lactobacillus using polysaccharide and beta glucan derived from Bacillus mycelia.
According to claim 1, Streptococcus genus, Lactococcus genus, Enterococcus genus, Lactobacillus genus, Pediococcus genus, Leuconostoc genus, Cellar ratio (Weissella) in Bifidobacterium and lactic acid bacteria to achieve binary one or two or more selected from the group consisting of (Bifidobacterium) in the strain.
The double-coated lactobacillus powder product of gluten-degraded lactobacillus using polysaccharide and beta glucan derived from Bacillus mycelium mycelium and flour-degraded cotton product using the same.
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| CN114107089B (en) * | 2021-10-22 | 2024-03-12 | 上海商学院 | Lactobacillus plantarum and method for extracting beta-glucan by fermentation and application thereof |
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