KR20190140717A - A method for producing a distillate of a herbal medicine - Google Patents

Abstract

The present invention relates to a method for manufacturing a distillate of medicinal herbs, wherein the distillate manufactured by the method of the present invention is easy to take inherent aroma and odor of medicinal herbs, and preserves inherent efficacy of medicinal herbs. The method for manufacturing the distillate of medicinal herbs and the distillate manufactured by the same has advantage of having a fast absorption rate and not having any side effects.

Description

A method for producing a distillate of a herbal medicine

The present invention relates to a method for preparing a distillate of herbal medicines.

According to ancient medical literatures such as Dongbobogam and herbal herb, various efficacy of our traditional natural herbal medicines have been reported since ancient times. Recently, the efficacy of these herbal medicines has been scientifically tested through these herbal medicines. In recent years, more and more patients are taking herbal medicines to treat incurable diseases that cannot be resolved.

As a way of extracting the natural herbal ingredients effective to our body, the traditional method of decoction has been used in the past. Traditionally, decoction method puts the herbal medicines together with water in a pot made of earthenware or porcelain, decoction for a long time, and then swallows the contents It was a way of squeezing out cloth.

However, when using this type of cooking method, since a large part of the ingredients of the medicine vaporizes and escapes to the outside, a large part of the active ingredient is lost and there is a problem that the surrounding environment is contaminated due to the peculiar smell that occurs during the cooking. In particular, there is a lot of problems to popularize because there is a sense of refusal to drink with the bitter taste, smell, etc. peculiar to herbal and herbal medicine from herbal medicine.

Recently, herbal medicines in the form of clear and transparent distillates have been removed by evaporating the herbal extracts, vaporizing them, cooling them, and extracting them to remove the inherent tastes, aromas, and odors.

However, these distillate-type herbal medicines are manufactured by a simple distillation process, and in fact, no active ingredients are present in the distillate, or are extremely insignificant. there was.

Accordingly, the present inventors have completed the present invention by preparing a distillate, which is easy to take the inherent aroma and odor of the herbal medicine by improving the distillation method by using dolwoo as a herbal medicine, and preserved the inherent efficacy of the herbal medicine.

Republic of Korea Patent No. 0524659 Republic of Korea Patent No. 0562460 Republic of Korea Patent Publication No. 2014-0137549

It is an object of the present invention to provide a method for preparing an extradol distillate.

The present invention provides a method for preparing a distillate of natural herbal medicines.

Specifically, the present invention provides a herbal medicine distillate manufacturing method comprising the following steps:

(1) preparing herbal liquid and water by putting the herbal medicine and water in a kettle;

(2) cooling the steam generated in the step of discharging to prepare a distillate;

(3) mixing the prepared distillate again into the discharging solution;

(4) repeating steps (2) and (3) to circulate the distillate; And

(5) extracting the final distillate after the circulation.

The known distillate manufacturing method of the herbal medicine was a method of directly extracting the distillate by liquefying the steam generated by heating the herbal medicine with water. However, the present inventors have confirmed that the distillate prepared by such a conventional method is significantly reduced inherent efficacy of the herbal medicine. Therefore, the present inventors, unlike the conventional method, by repeating the process of mixing the distillate obtained by cooling the herbal medicine and water generated by heating the water again to the discharging liquid to reduce the inherent aroma and odor of the distillate and taste It was possible to purify and increase the content of the desired functional ingredient.

The content of the herbal medicine and water used to prepare the distillate may be used in a 1 kg to 5 kg herbal medicine in an amount that can be prepared once by using the electric kettle, and the amount of water may be adjusted according to the amount of the herbal medicine. Can be.

In the method, the circulation time in the step (4) circulating the distillate may be 1 to 12 hours, preferably 7 to 8 hours. If the circulation time is less than 1 hour, sufficient functional components may not be extracted, and if the circulation time is 15 hours long, the functional components may be removed from the distillate again.

In this method, the time for extracting the distillate in step (5) may be 1 to 5 hours, preferably 3 hours.

In addition, the distillate production method of the present invention may further include the step of injecting hydrogen gas to the final extracted distillate. Hydrogen is known to have an excellent antioxidant effect, and by adding a certain concentration of hydrogen gas to the distillate of the present invention, it was confirmed that the taste and smell of the distillate were purified. In one embodiment of the present invention, the concentration of the hydrogen gas to be added may be adjusted according to the content of the herbal medicine, preferably a hydrogen gas of 500 to 1000 bbp concentration may be added.

The present invention provides a method for preparing a distillate comprising the following steps using herbal as a herbal medicine:

(1) preparing a pouring liquid by adding 3 kg of dol and 20,000 to 25,000 ml of water into a mixer and heating it;

(2) cooling the steam generated in the step of discharging to prepare a distillate;

(3) mixing the prepared distillate again into the discharging solution;

(4) repeating steps (2) and (3) to circulate the distillate for 8 hours;

(5) extracting the distillate for 3 hours after the end of the circulation; And

(6) injecting hydrogen gas into the extracted distillate.

The doldol distillate prepared by the above method was soft with less smell and aroma and less herb taste. It was confirmed that the antioxidant effect was high due to the high content of polyphenol and flavonoid.

Distillate manufacturing method of the herbal medicine according to the present invention and the distillate prepared by it can remove the inherent aroma, smell, and taste of the herbal medicine can be taken by anyone regardless of age and sex, it is composed of distillate When taken, the absorption rate is fast and there are no side effects.

Figure 1 shows the results of measuring the total polyphenol content of the first distillate sample.
Figure 2 shows the results of measuring the total polyphenol content of the secondary distillate sample.
Figure 3 shows the results of measuring the total flavonoid content of the secondary distillate sample.
Figure 4 shows the results of measuring the DPPH scavenging activity of the secondary distillate samples 4-A and 4-B.
Figure 5 shows the results of measuring the DPPH scavenging activity by concentration of the distillate re-reproduced the second distillate sample 4-B.
Figure 6 shows the results of measuring the superoxide radical scavenging activity according to the concentration of the extradol distillate.
Figure 7 shows the results of measuring the hydrogen peroxide scavenging activity according to the concentration of the extradol distillate.
Figure 8 shows the results of measuring the nitric oxide scavenging activity of each concentration of the extradol distillate.
Figure 9 shows the results of measuring the free radical production inhibitory activity due to the metal catalyst for each concentration of the extradol distillate.
Figure 10 shows the results of measuring the reducing power of each concentration of the extradol distillate.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the following examples.

Example  One. Stone  Distillate Preparation and Evaluation

The present inventors prepared an optimal distillate by variously adjusting the circulation time, extraction time, and the concentration of added hydrogen for the purpose of producing a distillate in the most optimal state using a dodol as a herbal medicine. The specific manufacturing process is as follows.

1. Preparation and Sensory Evaluation of Primary Distillate Sample

1kg of dodol outpost and 22,000 ~ 25,000ml of water were put into the condenser and the distillate was extracted by liquefying the water vapor generated when discharging at 106 ℃ using a cooler. Distillate extraction is to extract the distilled water liquefied immediately and to circulate and extract the distillate by repeating the process of distilling the distillate for a certain period of time using a circulator to mix with the distillate. Distillate was extracted by varying the circulation time 0, 30 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, 7 hours, 8 hours, 12 hours, 16 hours). . In addition, the distillate was extracted by varying the extraction time (1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours) to see the difference between the distillates according to the extraction time. In order to examine the difference of distillate according to the hydrogen concentration, the hydrogen concentration was measured by measuring the filling time of hydrogen concentration (1 minute, 2 minutes, 3 minutes, 4 minutes, 5 minutes in 500ml distillate). Hydrogen gas).

As a result, the sensory evaluation of the distillate according to the circulation time showed that the scent is strong when it is not circulated or circulated for a short time, and the taste of the herb is different, while the flavor decreases as the circulation time is increased and the taste is also removed. Rejection was reduced, it was confirmed that the color of the distillate darkened (Table 1 to Table 5). In particular, it was confirmed that the darkest color when cycled for 8 hours, the color fades again when cycled more time. This confirmed that the content of the components contained in the distillate was affected by the circulation time (Table 6).

In addition, as a result of the sensory evaluation of the distillate according to the extraction time, as the extraction time increases, the aroma decreases and the taste is also removed, the taste is removed, the rejection is reduced. In addition, the color was the darkest when extracted for 4 hours, and then gradually faded. This confirmed that the components contained in the distillate was also affected by the extraction time (Table 7 to Table 9).

As a result of sensory evaluation of the distillate according to the concentration of hydrogen, it was confirmed that the taste becomes softer as the concentration of hydrogen is increased (Table 10).

2. Preparation and Sensory Evaluation of Secondary Distillate Sample

Through the preparation of the primary sample, it was confirmed that the circulation time, extraction time, and hydrogen concentration affected the component and sensory evaluation of the distillate, and to establish more optimized dodol distillate preparation conditions based on the primary sample preparation result. A secondary sample was prepared for this purpose.

The secondary sample was put in 3kg of dodol outpost and 24,000 ~ 25,000ml of water in the electric kettle and liquefied water vapor generated at the time of discharging at 106 degrees using a cooler to extract the distillate. Distillate extraction is to extract the distilled water liquefied immediately and to circulate and extract the distillate by repeating the process of distilling the distillate for a certain period of time using a circulator to mix with the distillate. Distillate was extracted by varying the circulation time (5 hours, 6 hours, 7 hours, 8 hours) to determine the difference. In addition, the distillate was extracted by varying the extraction time (1 hour, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours) to see the difference between the distillates according to the extraction time. In order to examine the difference between the distillates according to the hydrogen concentration, the hydrogen concentration was measured by measuring the filling time of the hydrogen concentration (filling hydrogen gas into the 500 ml distillate, the hydrogen dissolved concentrations were 617ppb, 817ppb, 933ppb).

As a result, the sensory evaluation of the distillate according to the circulation time, as a result of increasing the circulation time, the fragrance was reduced to reduce the rejection, it was confirmed that the color of the distillate darkened (Table 11 and Table 12). In particular, it was confirmed that the darkest color when cycled for 8 hours, the color fades again when cycled more time. This confirmed that the content of the components contained in the distillate was affected by the circulation time (Table 6).

In addition, as a result of the sensory evaluation of the distillate according to the extraction time, there was little difference in taste, aroma and color as the extraction time increased, only the amount of extraction increased (Table 13). Considering the economic evaluation according to the amount of extraction and the difference in weakness according to the concentration of the extract, it is judged that the weakness of the concentrated concentration of the extract is excellent, so the extraction time is preferably 3 hours.

As a result of sensory evaluation of the distillate according to the concentration of hydrogen in the secondary sample, it was confirmed that the taste of the distillate was softer as the hydrogen concentration was increased, and the taste was the softest at the concentration of 900 ppb (Table 14).

Example  2. Stone  Component Analysis of Distillate Samples

Based on the results in Example 1, the component analysis of the distillate samples prepared under various conditions was confirmed by requesting the Korea Functional Food Research Institute. Ginseng saponins rb-1, rg1- and rg-3 contents and irradiated pontins were analyzed. Preparation of the distillate sample commissioned for analysis was carried out as follows and specific compositions and conditions are shown in Table 15 below.

A-1: The steamer was operated by putting 3 kg of stones and 25,000 ml of water in the mixer and setting the final heating temperature to 106 ° C. From the point where water vapor began to develop and the cooler started to operate, the distillate was extracted. The distillate was circulated continuously for 16 hours using a circulation device which pushes it back into the mixer. After 16 hours of circulation, the circuit was terminated and the sample made by collecting the distillate for 3 hours was named A-1.

A-2: The steamer was operated by putting 3 kg of stones and 25,000 ml of water in the mixer and setting the final heating temperature to 106 ° C. From the point where water vapor began to develop and the cooler began to operate, the distillate was extracted, and the distillate was circulated to the potter for 12 hours using a circulation device which pushes it back into the potter. After 12 hours of circulation, the circuit was terminated and the sample made by collecting the distillate for 3 hours was named A-2.

A-3: The steamer was operated by putting 3 kg of stone and 24,000 ml of water in the mixer and setting the final heating temperature to 106 ° C. Distillate begins to be extracted from the point where water vapor starts and the cooler starts to operate, and the distillate is circulated continuously for 8 hours using a circulation device which pushes it back into the mixer. After 8 hours of circulation, the circuit was terminated and the sample made by collecting the distillate for 3 hours was named A-3.

A-4: 3 kg of water and 24,000 ml of water were added to the mixer, and the final heater was set to 106 ° C to start the mixer. From the point where water vapor began to develop and the cooler started to operate, the distillate was extracted, and the distillate was circulated to the binner for 6 hours using a circulation device which pushes it back into the binner. After 6 hours of circulation, the circuit was terminated and the sample made by collecting the distillate for 3 hours was named A-4.

B-1: The steamer was operated by putting 3 kg of stone and 24,000 ml of water in the mixer and setting the final heating temperature at 106 degrees. When the distillate began to be extracted from the time when the steam began to be generated and the cooler started to operate, the sample was collected by distillate immediately after turning off the circulator and collecting the distillate for a total of 5 hours as B-1.

B-2: The steamer was operated by putting 3 kg of stone and 24,000 ml of water in the mixer and setting the final heating temperature at 106 degrees. When steam began to be generated and the distillate was extracted from the point where the cooler began to operate, the sample was collected by dividing the distillate for 3 hours by turning off the circulator and immediately collecting the distillate.

B-3: The water heater was operated by putting 3 kg of stone and 25,000 ml of water in the water mixer and setting the final heating temperature to 106 ° C. From the point where water vapor began to develop and the cooler started to operate, the distillate was extracted. The distillate was circulated continuously for 16 hours using a circulation device which pushes it back into the mixer. After 16 hours of circulation, the circuit was terminated and the sample made by collecting the distillate for 1 hour was named B-3.

B-4: The steamer was operated by putting 3 kg of stones and 25,000 ml of water in the mixer and setting the final heating temperature to 106 ° C. From the point where water vapor began to develop and the cooler started to operate, the distillate was extracted. The distillate was circulated continuously for 16 hours using a circulation device which pushes it back into the mixer. After 16 hours of circulation, the sample was prepared by collecting the distillate for 1 hour from 1 hour to 2 hours after the cycle was stopped and the distillate was collected.

B-5: 3 kg of water and 25,000 ml of water were added to the water mixer, and the final heating temperature was set to 106 ° C, and the water heater was operated. From the point where water vapor began to develop and the cooler started to operate, the distillate was extracted. The distillate was circulated continuously for 16 hours using a circulation device which pushes it back into the mixer. After 16 hours of circulation, the sample made by collecting the distillate for 1 hour from 2 hours to 3 hours after the end of the circulation device and collecting the distillate was named B-5.

B-6 (Sample for reproducibility of A-3 sample): 3kg of water and 24,000ml of water were added to the water heater and the final heating temperature was set to 106 ° C to operate the water heater. Distillate begins to be extracted from the point where water vapor starts and the cooler starts to operate, and the distillate is circulated continuously for 8 hours using a circulation device which pushes it back into the mixer. After 8 hours of circulation, the circuit was shut down and the sample made by collecting the distillate for 3 hours was named B-6.

The results of component analysis are shown in Table 16 below.

In the A-3 and B-6 samples, the content of ginsenoid and irradiated phononin was the highest. In addition, the sample was made to circulate the distillate for 8 hours and the distillate was extracted for 3 hours, the color appeared as dark as possible. Through this, it was confirmed that there is a change in the distillate component and content according to the distillate preparation conditions and methods.

Example  3. Stone  Antioxidant Effect and Stability Evaluation of Distillate

3-1. Sample Preparation

The first and second extradol distillate samples prepared by the method of Example 1 were used according to the conditions shown in Tables 17 and 18 below.

Organic solvent dolsal extract sample for comparison with distillate is pulverized by washing and drying the dolsal with distilled water (PVTFI 10A, Ilsin Lab, Korea), and the powdered sample is -80 ℃ ultra low temperature It was stored and used in the freezer. Each sample was extracted with active solvent with 100% methanol (MeOH) and 70% ethanol (EtOH) as the extraction solvent with an amount of 5 times the dry weight of solvent (g / mL, W / V). 100% MeOH and 70% EtOH were extracted for 72 hours using a shaking incubator at 150 rpm and 25 ℃. After the extraction time, the extra-dol samples were centrifuged at 1500 rpm for 15 minutes and suspended components were removed using a 0.45 μm filtration filter.

3-2 Determination of Total Polyphenol Content

Polyphenol compounds are flavonoids, anthocyanins, tannins, catechins, isoflavones, lignans, resveratrols, etc., and they are phytochemicals that are widely distributed in the plant kingdom, especially in large quantities in fruits and leafy vegetables. Resonance structure by many hydroxyl group (-OH) and phenol ring in polyphenol has the property of easily binding with various compounds, contributing to lipid oxidation, removal of active oxygen, and pharmacological effects such as antioxidant, anticancer and anti-inflammatory. Is known to be outstanding. Accordingly, pharmacological effects such as antioxidant effects can be predicted by measuring the content of the polyphenol compound contained in the extra distillate.

The total polyphenol content of the samples was determined by modifying the method of Folin-Denis. 50% Folin-Ciocalteu reagent, 95% EtOH, DW were added to the sample prepared by the above method. After 5 minutes, add 5% sodium carbonate solution, mix, shade for 1 hour, develop color, measure absorbance at 725 nm using microplate reader (Spectra MR, Dynex, VA, US), and use gallic acid standard solution. It was quantified by the calibration curve obtained.

As a result, the content of polyphenols contained in the primary dol distillate sample was measured. As a result, 4-A and 4-B were 13.1 and 12.4 mg gallic acid equivalence (GAE) / L, respectively. The cycle time seems to be a factor, but the total polyphenol content of all the samples was observed to be low (FIG. 1). The total polyphenol content of the secondary distillate samples was 6-7 times higher than that of the other samples, with 4-A and 4-B showing 322 and 334 mg gallic acid equivalence (GAE) / L, respectively. This high content is believed to be the main factor was the increase in the circulation time conditions, it was determined that the distillate extraction time and the hydrogen concentration is not (Fig. 2).

3-3 Determination of Total Flavonoid Content

Flavonoids belong to polyphenols, which are C6-C3-C6 skeletons and are yellow or light yellow phenolic compounds. They are found in almost all parts of vegetables, plants, leaves, flowers, fruits, stems and roots. In addition, it is abundant in nature, such as grains and fruits. Flavonoids, like polyphenols, are known to have high antioxidant capacity by effectively removing active oxygen species, and have been reported to act on various physiological activities. In the present invention, the flavonoid content of the extradol distillate was also measured. Specifically, 5% sodium nitrite solution and DW were added per 30 μL of the sample, and after 6 minutes, 10% aluminum chloride solution was added, followed by incubation at room temperature for 6 minutes. After adding 4% NaOH solution, the total volume was adjusted to DW, mixed well, and reacted at room temperature for 15 minutes. After the reaction time was completed, the absorbance was measured at 510 nm using a microplate reader, and quantified by a calibration curve obtained using rutin standard solution.

As a result, the flavoid content of the extradol distillate secondary sample was 203.5 and 206.6 mg rutin equivalence (RE) / L at 4-A and 4-B as shown in FIG. Indicated. As a result of the significantly high total flavonoid content, it was assumed that the increase in circulation time condition was an important factor as the total polyphenol content result.

3-4 Evaluation of Antioxidant Effects

Secondary distillate sample 4-B with high polyphenol and flavonoid content in the primary and secondary distillate samples was selected as a final distillate candidate and the reproducibility of the distillate efficacy and antioxidant activity were measured.

One. DPPH  Radical Scavenging power  Measure

The same amount of 4 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared immediately before the experiment was added to 100 µL of the sample solution prepared by diluting to various concentrations, and the mixture was shielded for 10 minutes at room temperature. The absorbance was measured by using a microplate reader at 517 nm and the scavenging ability was calculated by the following formula.

% inhibition = [{(C-CB)-(S-SB)} / (C-CB)] x 100;

S: absorbance of the sample, SB: Sample blank, C: control, CB: control blank

As a result, 4-A and 4-B of the secondary sample of the extradol distillate showed DP4.7 radical scavenging activity of 64.7% and 67.8% (FIG. 4). In particular, the 4-B was prepared again to improve reproducibility and activity. As a result of the measurement, the activity was increased in a dose-dependent manner, and the scavenging activity of 50.8, 55, 59.7, and 68.5% in 25, 50, 75, and 100% distillate, respectively (FIG. 5).

2. Superoxide  Radical scavenging activity

50 μl sodium carbonate buffer (pH 10.5), 10 μl of 3 mM xanthine, 10 μl of 3mM ethylenediamind tetraacetic acid (EDTA) and 10 μl of 0.15% bovine serum albumin solution were added to 10 μl of the sample and reacted at room temperature. After the reaction, add 100 μL of 6 mU / mL xanthine oxidase and react for 20 minutes at room temperature. Then, stop the reaction by adding 10 μL of 6 mM copper (I) chloride (CuCl) and measure absorbance at 560 nm using a microplate reader. Was calculated by the following formula

% inhibition = [{(C-CB)-(S-SB)} / (C-CB)] x 100;

S: absorbance of the sample, SB: Sample blank, C: control, CB: control blank

As a result, the superoxide radical scavenging ability of the extradol distillate also showed a concentration-dependent increase, but showed a higher scavenging activity of 87.7% when the 25% distillate was treated (Fig. 6).

3. Hydorogen  peroxide Scavenging power  Measure

After separating the sample, blank and control in 96 well plate, 100 μL of phosphate buffer solution (0.1 M, pH 5.0) and 20 μL of 10 mM H2O2 were mixed in 80 μL of each enzyme sample for 5 minutes in an incubator at 37 ℃. 30 μL of 1.25 mM 2,2-azinobis (3-ethylbenzthiazolin) -6-sulfonicacid (ABTS) prepared before the experiment and 30 μL of distilled water were added to the blank, followed by 30 μL of peroxidase (1 U / mL). After adding to the blank and reacted for 10 minutes in an incubator at 37 ℃, the absorbance was measured at 405 nm with a microplate reader.

As a result of measuring hydrogen peroxide scavenging ability of the extradol distillate, the distillate showed activity of 85.4% (FIG. 7).

4. Nitric oxide radical Scavenging power  Measure

50 μL of the sample was added to the same amount of 10 mM sodium nitroprusside prepared in phosphate buffer solution (pH 7.0) and incubated at 25 ° C. for 3 hours, followed by 100 μL Griess reagent (1% sulfanilamide and 0.1% N-naphthylethylene diamine dihydrochloride in 2.5% polyphosphoric acid) was mixed for 5 minutes and absorbance was measured at 540 nm.

% inhibition = [{(C-CB)-(S-SB)} / (C-CB)] x 100;

S: absorbance of the sample, SB: Sample blank, C: control, CB: control blank

As a result, as shown in Figure 8, the scavenging ability of 58.5, 61.6, 69.6, 75.4% in 25, 50, 75, 100% distillate, respectively.

5. Metal ion scavenging ability  Measure

Metal ions or metal salts containing Fe2 + or Cu2 + promote free radical generation, causing oxidative stress, and metal sequestration measures the ability to prevent lipid oxidation by inhibiting free radical generation by these metal catalysts. It can be used as an indicator.

In order to confirm the metal containment, the sample was dispensed into 250 well aliquots in 96 well plates, 5 μL of 2 mM ferrous chloride (FeCl 2), 5 mM ferrozine [3- (2-pyridyl) -5,6-diphenyl-1,2 , 4-triazine-p, p-disulfonic acid monosodium salt hydrate] solution was added 10 μL and reacted at room temperature for 10 minutes. The absorbance was measured by using a microplate reader at 562 nm, and the scavenging ability was calculated by the following formula.

% inhibition = [{(C-CB)-(S-SB)} / (C-CB)] x 100;

S: absorbance of the sample, SB: Sample blank, C: control, CB: control blank

As a result, the blocking power of the extra-distillate distillate increased proportionally with increasing sample concentration, confirming the scavenging ability of 53.4, 64.3, 69.3, and 75.9% in 25, 50, 75, and 100% distillate, respectively (FIG. 9). .

6. Reducing power measurement

Reducing power is confirmed by verifying Perl's prussian blue produced by reacting Fe3 + mixture with FeCl3 by antioxidants. To measure the reducing power of the extradol distillate, add 200 mM sodium phosphate buffer (pH 6.6) and 1% potassium ferricyanide equivalent to 400 μL of sample and react for 20 minutes at 50 ° C, and then stop the reaction by adding the same amount of 10% trichloroacetic acid. After the addition, 20 μL of 0.1% ferric chloride and 100 μL of DW were added to each sample, and the absorbance was measured at 700 nm.

% inhibition = [{(C-CB)-(S-SB)} / (C-CB)] x 100;

S: absorbance of the sample, SB: Sample blank, C: control, CB: control blank

As a result, the same trend as the electron donating ability was shown (Fig. 10).

Claims (6)

(1) preparing herbal liquid and water by putting the herbal medicine and water in a kettle;
(2) cooling the steam generated in the step of discharging to prepare a distillate;
(3) mixing the prepared distillate again into the discharging solution;
(4) repeating steps (2) and (3) to circulate the distillate; And
(5) extracting the final distillate after the circulation; distillate manufacturing method of the herbal medicine comprising a.
The method of claim 1,
Distillate manufacturing method of herbal medicines further comprising the step of injecting hydrogen gas to the final extracted distillate.
The method according to claim 1 or 2,
The circulation time of the distillate in the step (4) is 1 to 12 hours, distillate manufacturing method of the herbal medicine.
The method according to claim 1 or 2,
The time for extracting the distillate in the step (5) is 1 to 5 hours, distillate manufacturing method of the herbal medicine.
(1) preparing a pouring liquid by adding 3 kg of dol and 20,000 to 25,000 ml of water into a mixer and heating it;
(2) cooling the steam generated in the step of discharging to prepare a distillate;
(3) mixing the prepared distillate again into the discharging solution;
(4) repeating steps (2) and (3) to circulate the distillate for 8 hours;
(5) extracting the distillate for 3 hours after the end of the circulation; And
(6) a distillate manufacturing method other than stone comprising the step of injecting hydrogen gas to the extracted distillate.
The method of claim 5,
The concentration of the hydrogen gas in the step (5) is 900 to 1000 PPB, a distillate manufacturing method of the stone.

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