KR20190127260A - Reinforcement Method for Water Leaking of Water Facilities using Soil Microbes Biostimulation and Microparticles Injection - Google Patents

Abstract

The present invention relates to a water leakage reinforcing method of a waterside structure through connection of biostimulation and minute particle injection of indigenous microbes. The water leakage reinforcing method of a waterside structure of the present invention uses connection of biostimulation and minute particle injection of indigenous microbes so as to minimize danger of embankment damage in the waterside structure in which aging or water leakage is already generated and implement eco-friendly water leakage reinforcement.

Description

Reinforcement Method for Water Leaking of Water Facilities using Soil Microbes Biostimulation and Microparticles Injection}

The present invention relates to a leak reinforcement method for waterfront structures, such as reservoirs, dams, dams, reservoirs, or waterproofing levees, and more particularly, to a watertight reinforcement method of waterfront structures through linkages of living stimulation and particulate injection of indigenous microorganisms. It is about.

Since facilities such as soil dams and embankments may leak due to aging, high-level techniques for precise diagnosis and reinforcement are required. In particular, some fill dams are penetrated and leaked due to aging of the dam body, which may cause sudden dam destruction and large-scale disasters. Currently, the general repair technique based on cement grouting injection is most commonly used to repair and reinforce such leaks.However, when the general repair method based on cement grouting injection is applied to small facilities such as soil dams and embankments, Problems such as this can occur.

For example, cement grouting may cause damage to facilities such as hydraulic fracturing due to overinjection, environmental problems such as water pollution in lakes, There is a problem that can increase the probability of recurrence of leaks.

In order to solve such various problems that may occur during the construction and maintenance process, it is inevitable to develop an environment-friendly leak reinforcement technology to minimize the risk of body damage.

As a prior art document, the article "Cunningham, AB, Sharp, RR, Hiebert, R., & James, G. (2003). Subsurface biofilm barriers for the containment and remediation of contaminated groundwater. Bioremediation Journal, 7 (3-4) , 151-164. ", Or" Van Beek, VM, Den Hamer, D., Lambert, JWM, Latil, MN, & Van Der Zon, WH (2007, April). Biosealing, a natural sealing mechanism that locates and repairs leaks. In Proceedings of the 1th international conference on self healing materials, Noordwijk aan Zee, The Netherlands (pp. 18-20). " And the like can be referred to.

Accordingly, the present invention has been made to solve the above-described problems, the object of the present invention is to minimize the risk of body damage in the aging or water leakage structure that has already leaked, so that the environment-friendly leak reinforcement is made, the production of indigenous microorganisms The present invention provides a method for reinforcing a leak in a waterfront structure using a combination of magnetic poles and particulate injection.

First, to summarize the features of the present invention, the method of reinforcing the leaky water structure according to an aspect of the present invention for achieving the above object, by injecting a culture solution for the biostimulation of bacteria or microorganisms into the leak passage of the waterside structure bio A first leak reduction step of reducing leakage by inducing formation of materials to reduce pore size; And a second leakage reduction step of reducing leakage by injecting solid particles into the leakage flow path in which the biomaterial is formed to be trapped in the voids.

In addition, the leak reinforcement method of the waterfront structure according to another aspect of the present invention, including the step of injecting the culture medium for the biostimulation of bacteria or microorganisms into the leak channel of the waterfront structure, and injecting solid particles into the leak channel. In addition, by injecting the culture solution to induce the formation of biomaterials to reduce the pore size, the solid particles may be trapped by the pores can be reduced by leakage.

In the first leakage reduction step, the biomaterial including biopolymer, biofilm, or biomineral produced by the growth of the bacteria or microorganisms is promoted by the culture solution to reduce the pore size.

Before the first leakage reduction step, further comprising the step of preparing the culture medium containing a substance for promoting the growth of the bacteria or microorganisms inhabiting the area based on the soil sample collected around the leak passage.

The culture medium includes at least one of sucrose for promoting fermentation or yeast extract for supplying vitamin and promoting fermentation to produce a biopolymer. . Here, the content of sucrose (Sucrose) may be 30 ~ 300g / L depending on the permeability to reach. The culture medium for producing the biopolymer may further include a phosphate buffer for a predetermined optimal pH composition.

The culture medium includes any one or more of fumarate or nitrate, which can act as an electron acceptor to enable anaerobic respiration of bacteria or microorganisms to produce a biofilm. The culture solution may further include any one or more of Casein, Soya peptone, NaCl, Dipotassium phosphate or Dextrose depending on the type of bacteria or microorganism. The culture medium for producing the biofilm may further include a phosphate buffer (Phosphate buffer) for a predetermined optimal pH composition.

In the second leakage reduction step, the solid particles including silt or clay having a diameter small enough to be penetrated into a region where the biomaterial is formed are injected.

The solid particles are selected and injected to be adjusted at a ratio between 1-10% (mass ratio) concentration per predetermined liter of water according to a target permeability, and the size of the solid particles is 50 micrometers or less in diameter.

According to the leak reinforcement method of the waterfront structure according to the present invention, by-products (eg, biofilm, biopolymer, etc.) can be generated primarily through biostimulation of indigenous microorganisms to reduce leakage, and the first leakage reduction is completed. Secondary leakage reduction is achieved by injecting additional particulates (eg, clay, silt, etc.) where the by-products are generated, minimizing the risk of body damage in aging or already leaking waterside structures, and making environmentally friendly reinforcement. Can be lost.

In other words, by supplying nutrients suitable for propagation of indigenous microorganisms already inhabiting waterside structures such as reservoirs, dams, dams, and levees, the production of biomaterials (e.g. biofilms, biopolymers, etc.) is promoted primarily. Leakage can be reinforced. Furthermore, in order to enhance the primary leakage reduction effect by biomaterials, secondary leakage reduction effects can be obtained by injecting fine particles that are harmless to an environment such as silt or clay at a point where the permeability is reduced by the production of by-products.

In this way, water leakage is reduced by using by-products generated by stimulating the metabolism of microorganisms living in existing waterside structures such as soil dams, thereby improving economic efficiency by replacing existing grouting techniques and preventing water pollution through environmentally friendly materials. It also has the effect of increasing the life of the facility through prevention of core damage. In addition, double permeability reduction effect can be expected by injecting additional fine particles into the site where the water permeability is reduced.

BRIEF DESCRIPTION OF THE DRAWINGS The accompanying drawings, which are included as part of the detailed description in order to provide a thorough understanding of the present invention, provide examples of the present invention and together with the description, describe the technical idea of the present invention.
1 is a cross-sectional view of a waterfront structure for explaining the concept of the leak path reinforcement method of the waterfront structure according to an embodiment of the present invention.
2 is a view for explaining the primary, secondary leak reduction method according to the leakage flow path reinforcement method according to an embodiment of the present invention from the leakage of the waterfront structure.
3 is a photograph of examples of the appearance of the biopolymer Dextran according to the concentration of bacteria.
4 is an example of an enlarged photograph of a biopolymer.

Hereinafter, with reference to the accompanying drawings will be described in detail with respect to the present invention. In this case, the same components in each drawing are represented by the same reference numerals as much as possible. In addition, detailed descriptions of already known functions and / or configurations are omitted. The following description focuses on parts necessary for understanding the operation according to various embodiments, and descriptions of elements that may obscure the gist of the description are omitted. In addition, some components of the drawings may be exaggerated, omitted, or schematically illustrated. The size of each component does not entirely reflect the actual size, and thus the contents described herein are not limited by the relative size or spacing of the components drawn in the respective drawings.

In describing the embodiments of the present invention, when it is determined that the detailed description of the known technology related to the present invention may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted. In addition, terms to be described below are terms defined in consideration of functions in the present invention, which may vary according to the intention or custom of a user or an operator. Therefore, the definition should be made based on the contents throughout the specification. The terminology used in the description is for the purpose of describing particular embodiments only and should not be limiting. Unless expressly used otherwise, the singular forms “a,” “an,” and “the” include plural forms of meaning. In this description, expressions such as "comprises" or "equipment" are intended to indicate certain features, numbers, steps, actions, elements, portions or combinations thereof, and one or more than those described. It should not be construed to exclude the presence or possibility of other features, numbers, steps, actions, elements, portions or combinations thereof.

In addition, terms such as first and second may be used to describe various components, but the components are not limited by the terms, and the terms are used to distinguish one component from another component. Used only as

1 is a cross-sectional view of a waterfront structure for explaining the concept of the leak path reinforcement method of the waterfront structure according to an embodiment of the present invention.

Referring to FIG. 1, in the leak channel reinforcement method of the waterfront structure according to an exemplary embodiment of the present invention, when a water leakage occurs in the waterfront structure 100, a soil sample around the waterfront structure 100 is collected and a corresponding region is based on this. Analyze the colonies of indigenous bacteria or microorganisms inhabiting them, prepare a culture solution containing a substance that promotes the growth of the bacteria or microorganisms, and then perform each step for reducing the first and second leaks using the same. Process.

In the first leakage reduction step, by injecting the culture medium prepared for the biostimulation of bacteria or microorganisms as above into the leakage flow path 130 of the waterfront structure 100, a biomaterial (e.g., a biopolymer) generated to have a larger volume , Leakage of water by reducing the pore size by inducing the formation of biofilms or bio minerals).

In the second leakage reduction step, leakage is reduced by injecting solid particles such as silt or clay into the leak passage 130 in which biomaterials (eg, biopolymers, biofilms, or biominerals, etc.) are formed to be trapped in the voids.

The first leak reduction step may vary slightly depending on the species of indigenous bacteria or microorganisms that live in the area, but the biomaterials (eg, biopolymers, biofilms or biominerals, etc.) are sufficient for several days to several tens of days after the culture is injected. It is necessary to wait for) to form. In the first leak reduction step, the growth of bacteria or microorganisms is promoted by the culture medium to form biomaterials including biopolymers, biofilms, biominerals, and the like, thereby reducing the size of the voids present in the leak passage 130. have.

After the biomaterial (for example, biopolymer, biofilm or bio mineral, etc.) is sufficiently formed, in the second leak reduction step, the diameter is large enough to allow penetration into the corresponding region of the leak passage 130 in which the biomaterial is formed. By injecting solid particles containing small silt or clay to be trapped in the pores it is possible to reduce the leakage.

2 is a view for explaining a primary, secondary leak reduction method according to the leak path 130 reinforcement method according to an embodiment of the present invention from the leakage of the waterfront structure (100).

For example, first, as shown in Figure 2, the cracks are connected in accordance with the aging of the waterfront structure 100, such as soil dams or embankments, the leakage flow path 130 is generated, the water upstream can leak to the downstream side, Such leaks may increase more and more while creating larger voids, which may cause sudden dams or dams, and large-scale disasters (S110). In order to detect leaks in the waterfront structure 100 such as an earth dam or embankment, it is inaccurate only by visual observation, and special electronic equipment for detecting leaks by measuring specific resistance or potential may be used.

When a leak occurs in the waterfront structure 100, samples of soil around the waterfront structure 100 are collected, and based on the analysis, a population of indigenous bacteria or microorganisms inhabiting the area is analyzed and a substance that promotes the growth of the bacteria or microorganisms is identified. Prepare a culture solution containing. However, in anticipation of such leakage, a culture solution containing a substance for promoting growth of the bacteria or microorganisms on the waterfront structure 100 may be prepared in advance. The waterside structure 100 such as an soil dam or a levee is inhabited with various kinds of indigenous microorganisms capable of producing biomaterials (eg, biofilms and biopolymers). After collecting a sample of the soil around the waterfront structure (100) through the cluster analysis can identify the bacterial species inhabiting the area and to prepare a culture that can promote the growth of the species.

In the first leakage reduction step (S120), by injecting the culture medium prepared for the biostimulation of bacteria or microorganisms as above into the leakage flow path 130 of the waterside structure 100 biomaterials (eg, biopolymers, biofilms or bio To reduce the leakage by inducing the formation of minerals). When a culture solution for promoting the metabolic activity of bacteria or microorganisms is injected along the leakage passage 130 in a body in which bacteria or microorganisms live, primary leakage may be reduced through biomaterials generated by indigenous microorganisms. have.

The first leak reduction step may vary slightly depending on the species of indigenous bacteria or microorganisms that live in the area, but the biomaterials (eg, biopolymers, biofilms or biominerals, etc.) are sufficient for several days to several tens of days after the culture is injected. It is necessary to wait for) to form. In the first leak reduction step, the growth of bacteria or microorganisms is promoted by the culture medium to generate biomaterials (eg, biopolymers, biofilms, or biominerals) to fill the voids between the soil existing in the leak passage 130. It can reduce the size of the pores and reduce the permeability.

The culture medium for the biostimulation of bacteria or microorganisms is selected from various nutrients such as sucrose, yeast extract, Tryptic Soy Broth (TSB) and Nitrate (NO ₃ ) depending on the species of bacteria or microorganisms. Can be used.

For example, the most representative biopolymer, Dextran, is produced by the fermentation mechanism of the producing bacterium Leuconostoc mesenteroides, and sucrose for the promotion of fermentation mechanisms, or for the promotion of fermentation mechanisms and vitamins. Culture medium containing yeast extract (Yeast extract) and the like can be used. Dextran is produced from sugar. Sucrose acts as a sugar (for economic reasons, Sucrose can be replaced with sugar used in real life), and Yeast extract provides vitamins necessary for the growth of bacteria and helps transform sugar into Dextran. do. The amount of sucrose can be adjusted according to the permeability to be reduced. For example, the content of sucrose (Sucrose) in the total culture solution may be 30 ~ 300g / L. In the culture medium for the production of the biopolymer, a phosphate buffer may be further added for a predetermined optimal pH composition. Figure 3 shows a photograph of examples of the appearance of the biopolymer Dextran according to the concentration of bacteria. 4 is an example of an enlarged photograph of a biopolymer.

In the case of Bacillus subtilis, a biofilm-producing bacterium, fumarate or nitrate (NO ₃ ), which can act as an electron acceptor to allow anaerobic respiration of bacteria or microorganisms in an oxygen-deficient region, etc. Culture medium containing the can be used. In addition, depending on the type of bacteria or microorganism, the culture medium for producing the biopolymer may include Tryptic Soy Broth (TSB), and the TSB culture medium may include Casein, Soya peptone, NaCl, Dipotassium phosphate, Dextrose, or the like. It is a substance that contains nutrients necessary for cultivation. The culture solution for the production of the biofilm may be further added to the phosphate buffer (Phosphate buffer) for a predetermined optimal pH composition.

However, leakage reduction technology using indigenous microorganisms may be less effective in low temperature periods such as winter due to the characteristics of temperature-sensitive microorganisms, and may deteriorate when supply of nutrients is stopped.

In order to alleviate this inconvenience, in the second leakage reduction step (S130), the solid particles of the fine particles such as silt or clay into the leakage flow path 130 in which the biomaterial (eg, biopolymer, biofilm, or bio mineral) is formed. Water is reduced by injecting it into the voids. At this time, the leakage can be reduced by injecting solid particles containing silt or clay having a diameter small enough to be penetrated into a corresponding region of the leak passage 130 in which the biomaterial is formed so as to be trapped in the pores. For example, the solid particles are selected and injected to be adjusted at a ratio between 1-10% (mass ratio) concentration per predetermined liter of water according to the target permeability, and the size of the solid particles is preferably 50 micrometers or less in diameter.

In this way, in order to inject the culture medium and solid particles such as silt or clay in the first leakage reduction step (S110) and the second leakage reduction step (S120), from the upper side of the waterside structure (100). After the pipes 110 and 120 are inserted through the drilling operation to the location of the leaked oil passage 130, the culture solution and the silt / clay may be injected through the pipes. The pipes 110 and 120 may be selected in various numbers according to the state of the leak passage 130. For example, pipes 110 and 120 are installed at predetermined intervals (for example, 5 m intervals when viewed from the top of the waterside structure) starting from the upstream leakage start point and the downstream leakage end point. Silt / clay may be injected. Larger voids allow more pipes to be installed at relatively smaller distances.

As described above, the present invention enables the secondary leakage reduction effect by flowing the fine particles to the point where the biomaterial is generated. Fine particles refer to solid particles whose diameter is measured in micrometers (eg, 50 micrometers or less), such as silt or clay. These microparticles are free to flow inside the soil before biomaterials are produced, but when biomaterials are generated and the voids in the soil are reduced, the flow is restricted and consequently trapped in the pores, resulting in secondary leakage reduction.

As described above, according to the leak reinforcement method of the waterfront structure according to the present invention, by-products (eg, biofilm, biopolymer, etc.) can be generated primarily through the biostimulation of indigenous microorganisms, and the leakage can be reduced. Secondary leakage reduction is achieved by injecting additional particulates (e.g. clay, silt, etc.) at the location where the by-products have been reduced, minimizing the risk of body damage in aging or already leaking waterside structures, and Leak reinforcement can be made. In other words, by supplying nutrients suitable for propagation of indigenous microorganisms already inhabiting waterside structures such as reservoirs, dams, dams, and levees, the production of biomaterials (e.g. biofilms, biopolymers, etc.) is promoted primarily. Leakage can be reinforced. Furthermore, in order to enhance the primary leakage reduction effect by biomaterials, secondary leakage reduction effects can be obtained by injecting fine particles that are harmless to an environment such as silt or clay at a point where the permeability is reduced by the production of by-products.

In this way, water leakage is reduced by using by-products generated by stimulating the metabolism of microorganisms living in existing waterside structures such as soil dams, thereby improving economic efficiency by replacing existing grouting techniques and preventing water pollution through environmentally friendly materials. It also has the effect of increasing the life of the facility through prevention of core damage. In addition, double permeability reduction effect can be expected by injecting additional fine particles into the site where the water permeability is reduced.

Although the first leakage reduction step and the second leakage reduction step have been described above, the present invention is not limited thereto, and the injection of the culture medium and the solid particle injection in the secondary leakage reduction step may be performed at the same time. For example, the biomaterial is formed by injecting the culture solution even when the culture medium for the biostimulation of bacteria or microorganisms is injected into the leakage passage 130 of the waterside structure, and the solid particles are injected into the leakage passage 130. By reducing the pore size, the pore size can be reduced over time. At the same time, more solid particles are trapped in the pores by the formation of more biomaterials so that the leakage reduction effect can be increased over time. have.

In the present invention as described above has been described by the specific embodiments, such as specific components and limited embodiments and drawings, but this is provided to help a more general understanding of the present invention, the present invention is not limited to the above embodiments. For those skilled in the art, various modifications and variations may be made without departing from the essential features of the present invention. Accordingly, the spirit of the present invention should not be limited to the described embodiments, and all technical ideas having equivalent or equivalent modifications to the claims as well as the following claims are included in the scope of the present invention. It should be interpreted as.

Claims (12)

A first leakage reduction step of reducing leakage by injecting a culture solution for biostimulation of bacteria or microorganisms into the leakage passage of the waterside structure to induce the formation of biomaterials to reduce the pore size; And
Secondary leakage reduction step of reducing leakage by injecting solid particles into the leakage flow path in which the biomaterial is formed to be trapped in the air gap
Leak reinforcement method of the waterfront structure comprising a. And injecting a culture solution for biostimulation of bacteria or microorganisms into the leak passage of the waterside structure, and injecting solid particles into the leak passage.
Leakage reinforcement method of the waterfront structure, characterized in that to reduce the pore size by inducing the formation of the bio-material by the injection of the culture medium, the leakage by the solid particles are trapped in the pores. The method of claim 1,
In the first leakage reduction step, the biomaterial including a biopolymer, a biofilm, or a biomineral produced by the growth of the bacteria or microorganisms is promoted by the culture medium, thereby reducing the pore size. Leak reinforcement method of waterside structures. The method of claim 1,
Before the first leak reduction step,
Preparing a culture solution including a substance that promotes the growth of the bacteria or microorganisms inhabiting the region based on the soil sample collected around the leak passage;
Leak reinforcement method of the waterfront structure characterized in that it further comprises. The method of claim 4, wherein
The culture solution, in order to produce a biopolymer,
Sucrose (Sucrose) for the promotion of the fermentation mechanism, or a yeast extract (Yeast extract) for supplying vitamins (Vitamin) for promoting the fermentation mechanism (Leak) reinforcement method of the waterfront structure characterized in that it comprises at least one. The method of claim 5,
Sucrose (Sucrose) content of the water reinforcement method of the waterfront structure, characterized in that 30 ~ 300g / L depending on the permeability to reach. The method of claim 5,
The culture medium further comprises a phosphate buffer (Phosphate buffer) for the predetermined optimal pH composition of the water leakage reinforcement method characterized in that it further comprises. The method of claim 4, wherein
The culture medium, in order to produce a bio-film, of the waterfront structure comprising any one or more of fumarate (Fumarate) or nitrate (Nitrate) capable of acting as an electron acceptor to enable anaerobic respiration of bacteria or microorganisms Leak reinforcement method. The method of claim 8,
The culture solution, reinforcement method of the waterfront structure further comprises any one or more of Casein, Soya peptone, NaCl, Dipotassium phosphate or Dextrose according to the type of bacteria or microorganisms. The method according to claim 8 or 9,
The culture medium further comprises a phosphate buffer (Phosphate buffer) for the predetermined optimal pH composition of the water leakage reinforcement method characterized in that it further comprises. The method of claim 1,
In the second leakage reduction step, the leak reinforcement method of the waterfront structure, characterized in that the injection of the solid particles comprising a silt or clay having a diameter small enough to penetrate into the area where the biomaterial is formed. The method of claim 11,
The solid particles are selected and injected to be controlled at a ratio between 1-10% (mass ratio) per 1 L of water according to a target permeability, and the size of the solid particles is less than 50 micrometers in diameter leaking water structure Reinforcement method.

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