KR20190097244A - How to convert plant materials into plant extracts with antibacterial activity - Google Patents

Abstract

The present invention provides a method of converting a plant material into a plant extract having antimicrobial activity, wherein the method comprises grinding at least one plant material in a first step, with or without a medium, to an average of 10 to 1000 μm. Obtaining particles having a particle size; Mixing the particles with the second medium in a second step to obtain a mixture comprising bioactive compounds, and separating the mixture from the plant residues by separating the mixture. .

Description

How to convert plant materials into plant extracts with antibacterial activity

The present invention relates to a method of converting a plant material into a plant extract having antimicrobial activity. The present invention also relates to plant extracts derived from plant materials which possess antibacterial activity. The invention further relates to the use of said extract in cosmetics, hygiene products, nutraceuticals, foodstuffs and food supplements, feeds, packaged foods and pharmaceuticals.

Significant amounts of waste, side streams and by-products are formed in industries dealing with the processing of plant materials, such as the food industry for processing berries and fruits. The food industry uses wild berry, cultivated berry and fruit in the manufacture of a wide range of products such as pastes, beverages, liquor products, jams, conserves, dairy products, sweets and the like. In addition, the berry and fruit fractions are very widely used in cosmetics, for example, cloudberry seed oil rich in polyunsaturated fatty acids has attracted attention as a beneficial ingredient in cosmetic preparations.

Particularly in the food industry, large quantities of waste are produced when processing plant materials such as berries and fruits, and the use of these wastes is very rare. Most of the waste is now disposed of or transported to landfills or dump sites, increasing the environmental burden. Some of these wastes are seeded after drying, but only a very small proportion of the wastes are utilized.

Typically, during processing, the berry and fruit are machine washed and then the juice, pulp or paste is removed by a suitable method such as pressing. Residual waste, such as pomace, berry cake, fruit cake or press cake, may be a skin of berry or fruit, containing fibers, bioactive phenolic compounds and other bioactive compounds. ), Peels, seeds and piths.

FI 122664 B discloses a method for separating nutrients from fractions formed by fractionating berry, which dry and lightly crush the berry raw material, without breaking the berry's seeds After separation from the pulp and rind fractions from which the seeds have been removed, light secondary grinding is performed to form fine powders which are then screened or sorted. Seeds are not discerned this way. Discard the seeds and separate the shells.

US 2013/0040005 A1 relates to an antihypertensive comprising a boysenberry seed extract as an active ingredient and a method for obtaining said preparation. In the above method, the compressed residue of Boysonberry is dried, crushed and sieved to separate the seeds, and then the seeds are ground to form a fine powder, which is extracted with water or an organic solvent, and then the extract is contacted with a polyphenol absorbent and alcohol Elution with system solvent yields the target extract.

In particular, in cosmetics, skin care products, household goods and hygiene products, there is a need to replace synthetic preservatives such as parabens and triclosan with natural preservatives. The use of preservatives currently in use is expected to be limited in the future.

In view of the above situation, there still appears to be a need to provide a safe natural preservative to replace the preservative currently in use and to provide a method for producing a product having antimicrobial activity.

In the present invention, it has been found that plant extracts possessing antimicrobial activity can be obtained using a simple and environmentally safe method.

The invention particularly relates to seed, berry, fruit, leaf, debris, compressed cakes, berry cakes, fruit cakes, sediments obtained from side streams comprising leaves, berry and / or fruit and / or fats and oils, and combinations thereof. Based on a combination of grinding and processing of plant materials selected from the group consisting of, and the use of the products obtained. These materials are rich in dietary fiber and phenolic compounds that possess antioxidant and / or antimicrobial activity.

The present invention provides a very convenient and effective method for using seeds, leaves, wastes, side streams and by-products, particularly in industries that use plant materials such as berry and fruit, and the oil and vegetable industry, wherein the plant material is a cosmetic, skin care Seeds, berries, fruits, leaves, compresses in the manufacture of plant extracts with very high antimicrobial activity useful in products, household goods, hygiene products, food supplements, foodstuffs, feedstuffs, packaged foods and pharmaceuticals, especially as natural antimicrobials or natural preservatives. May include dregs, compressed cakes, berry cakes and fruit cakes, or precipitates.

It is an object of the present invention to provide a method for converting a plant material into a plant extract having antimicrobial activity.

It is a further object of the present invention to utilize seed, berry, fruit, leaf, waste, sidestream and by-products of industries that use plant materials without the need for disposal.

It is a further object of the present invention to provide a plant extract that retains antioxidant and / or antimicrobial activity from plant materials.

It is a further object of the present invention to have antioxidant and / or antimicrobial activity in cosmetics, skin care products, hygiene products, household products, nutraceuticals, foodstuffs, food supplements, feeds, pet foods, packaged foods and pharmaceuticals. Is to use plant extracts.

The present invention relates to a method for converting a plant material, wherein the method mills at least one plant material in the first step, in the presence or absence of the first medium, from 1 to 5 times, with an average particle size of 10 to 1000. To obtain particles which are μm;

In a second step, the particles are mixed with the second medium at a weight ratio of 1: 3 to 1:80 at a temperature of 10 to 120 ° C. for 20 minutes to 20 hours to obtain a mixture including the bioactive compound, and the mixture Separating the plant extract from the plant residue by separating it.

The plant extracts obtained are combined with cosmetics, skin care products, hygiene products, household products, nutraceuticals, foodstuffs, food supplements, feeds, pet foods, packaged foods and pharmaceuticals, or in the manufacture of these products. It can be combined with the starting material.

Antimicrobial active compounds include, in particular, phenolic compounds having antimicrobial activity, such as ellagic acid and ellagitannin.

The present invention further relates to the use of said plant extracts in cosmetics, skin care products, hygiene products, household goods, nutraceuticals, foodstuffs, food supplements, feeds, pet foods, packaged foods and pharmaceuticals.

Accordingly, the present invention relates to the production of plant extracts with antimicrobial activity for providing natural preservatives, which include plant materials, such as seeds, leaves, wastes, side streams, originating from industries processing plant materials, such as the berry industry and the fruit industry. And a simple and economical means of using by-products.

 Features of the invention are set forth in the appended claims.

Figure 1 shows the antimicrobial effect of Staphylococcus aureus of the plant extract obtained by the method of the present invention.
Figure 2 shows the antimicrobial activity of Escherichia coli (Escherichia coli) of the plant extract obtained by the method of the present invention.
Figure 3 shows the antimicrobial effect of Staphylococcus aureus of the plant extract obtained by the method of the present invention.
Figure 4 shows the antimicrobial effect of Pseudomonas aeruginosa of the plant extract obtained by the method of the present invention.
Figure 5 shows an HPLC chromatogram for the hydrothermal extract of raspberry leaves.
Figure 6 shows specific spectra for the main compounds of hydrothermal extracts of raspberry leaves.
Figure 7 shows the antimicrobial effect of Staphylococcus aureus of the hydrothermal extract of crushed raspberry leaves.
8 shows an HPLC chromatogram of hydrothermal extraction residue.

Justice

Unless otherwise stated, the terms used in this specification and claims have the meanings commonly used in the food industry and in the industry of processing plant materials. Specifically, the following terms have the meanings indicated below.

The term "berry" means any edible wild berry and cultivated berry that contains seeds therein, such as Rubus, Sorbus, Empetrum, Rosa, Ah. It belongs to Aronia, Vaccinium, Ribe, Fragararia, Juniferus or Hippophae and includes all hybrids of the genus. Sea buckthorn is an example of the genus Hippopie. Raspberry, blackberry, Arctic Bramble (arctic bramble), Dewberry (dewberry) and cloud berries are examples of rubeoseu species. Because the Rubus species are readily heterocrossed and apomict, the lineage of the hybrid plant is often very complex, but is generally included in the definition of cultivars of blackberries and raspberries. Examples of the hybrid berry are loganberry, loganberry, beechberry, marionberry, marionberry, silvanberry, tayberry, tummelberry and hilderberry ( hildaberry). Bilberry (bilberry), blueberries, blackberries ringgon (lingonberry) and cranberries are examples of back shinny Titanium species. Blackcurrants, redcurrants, whitecurrants, greencurrants, and gooseberries are examples of live species. Examples of Jounieh Peru's species may include juniper (juniper) (dusong, Juniperus communis).

The term "fruit" is used herein as Vitis, Punica, Pyrus, Malus, Citrullus, Benincasa, and Cucumis. It refers to all wild and cultivated fruits belonging to the genus, Mormordica, Olea and Argania spinosa, and including all hybrid fruit of the genus or species.

In the non-Tees fruits are mainly non-Tees Vinny Ferraro (V. vinifera) and also means that the grapes inside, including any hybrids of two kinds of hybrids of grapes and other vines, including seeds. Grapes are used to make wine, jams, beverages, jelly, seed extracts, raisins, vinegar and grapeseed oil. Non-tooth is made of a chisel 60 branches of vines and the plant belonging to vitaceae (Vitaceae) series.

Fruits of the genus Citrullus, Beninkasa, Cucumis and Momordica refer to melon, which contains seeds inside, including all hybrid melons.

Ole Ah ah in the fruit means (Olea europaea) Olive, especially Ole Ah Europa.

The fruit is a pomegranate [Fu Nika Gras natum (Punica granatum)], pear - pie Russ as, for example, a pie Russ communication bus (Pyrus communis)] and apple trees (horse Russ Division) and the fruit of their fruit trees, like all hybrids Refers to.

The term "maintain plant" means a plant included in the present vegetable oil-rich seeds and the plant is turnip rapeseed [Brassica rapa (Brassica rapa)], sunflower, flax, hemp [Cannabis sativa (Cannabis sativa)] Or rapeseed [ Brassica napus ].

The terms " berry industry " and " fruit industry " and " oil plant industry " refer to industries dealing with the development, purification and manufacture of products and processes related to wild berry and cultivated berry and fruit and oily plants.

The term "antimicrobial activity" compound refers to a compound capable of killing microorganisms or inhibiting their growth. Examples of such compounds include phenolic compounds such as ellagic acid and ellagitannin. In addition to antimicrobial and preservative activity, these compounds often possess other biological activities, in particular antioxidant activity.

The term "hydrothermal" processing herein means processing in the presence of water at elevated temperatures.

Sanitary articles herein include, in particular, diapers, feminine hygiene pads, tampons, incontinence pads and products and the like.

Home appliance herein refers to dishwashers, household cleaners, liquid soaps, shower gels, and the like.

Mechanical parts herein refer to membranes and filters in industrial and household washing machines and dishwashers.

Detailed description of the invention

The present invention provides a convenient method of converting a plant material into a plant extract having antimicrobial activity.

In the present invention, the plant extracts obtained by the method have been found to exhibit particularly strong antibacterial activity against, for example, Staphylococcus aureus , a human pathogen.

Recent studies have shown that phenolic compounds, such as ellagic acid and ellagitannin, have many interesting biological activities and may play a prophylactic role in disease prevention.

A significant amount of biologically active phenolic compounds, in particular ellagic acid and / or ellagitannin and derivatives thereof, are included in the plant material. For example, the compounds are not currently used while remaining in the waste. Phenolic compounds, such as ellagic acid and ellagitannin, are natural antioxidants and preservatives with strong antimicrobial effects, and therefore, natural preservatives in various applications of the cosmetics, hygiene, food and feed industries, as well as the packaging and pharmaceutical industries. It is useful as an antioxidant.

In cosmetics, skin care products and hygiene products, the present invention can be used to convert plant extracts to stabilize, balance and protect the microbiome of healthy skin, which is the growth of beneficial microorganisms. This is because it exhibits antimicrobial activity that inhibits the growth of pathogenic microorganisms without affecting.

Using the method of the present invention, the plant material is ground to obtain fine particles as a dry powder or suspension, and the particles are subjected to a separation process after extraction, thereby obtaining plant extracts and plant residues having high antimicrobial activity. do.

In particular, the present invention relates to a method for converting a plant material, wherein the method mills at least one plant material in the first step, in the presence or absence of the first medium, from 1 to 5 times, thereby achieving an average particle size of 10 To obtain particles that are from 1000 μm;

In a second step, the particles are mixed with the second medium at a weight ratio of 1: 3 to 1:80 at a temperature of 10 to 120 ° C. for 20 minutes to 20 hours to obtain a mixture including the bioactive compound, and the mixture Separating the plant extract from the plant residue by separating it.

The plant residue isolated from the plant extract after extraction can be used especially for nutraceuticals, foodstuffs, food supplements, pet food and feed due to the high content of ellagic acid, C18 fatty acids, lignin and fiber, The solid particles contained in water make them suitable for use in cosmetics, especially masks and exfoliating products. Ellagic acid is generally absorbed better in the gastrointestinal tract than ellagitannins present in natural berries and fruits.

Plant material

The plant material is selected from seeds, berries, fruits, leaves, chips, compressed cakes, berry cakes, fruit cakes, and wastes, sediments, side streams and by-products from the industry of processing plant materials, and any combination thereof. do.

In one embodiment, the plant material is selected from berry material, fruit material, oily vegetable material and combinations thereof.

The berry material is selected from seeds, berry, leaves, compacts, compressed cakes, berry cakes, and wastes, sediments, side streams and by-products from the industry of processing berry materials, and any combination thereof.

The fruit material is selected from seeds, fruits, leaves, compacts, pressed cakes, fruit cakes, and wastes, sediments, side streams and by-products from the industry of processing fruit materials, and any combination thereof.

The oil or vegetable material is selected from wastes, sediments, side streams and by-products from the industry of processing seeds, fruits, leaves, compacts, compressed cakes, fruit cakes, and oil and vegetable material, and any combination thereof.

Berry

In the present invention, rubeoseu, Mysore bus, M. Petrov room, Rosa, Chokeberry, bag Shinny Stadium, Raleigh beads, the plastic is Leah, gave Peru's and hippo all edible wild blueberries in the pie, cultivated blueberries and all hybrid berry, and Any combination of these can be used. Hybrid species berry including raspberry, blackberry, arctic scramble (synonymous with arctic raspberry), duberry and cloudberry, and loganberry and voyagerberry are examples of Rubus species suitable for the present invention. Roeon Berry (rowanberry) is an example of the Mysore Bus species, Crow Berry (crowberry) is M. and examples of pet room servant, it rose hip (rose hip) and dog rose (dog rose) is an example of Rosa species, choke berry ( chokeberry) Oh, and examples of the kinds of Catalonia, the living tree berry are examples of these kinds of Hippo pie is suitable for this invention. Bilberries, blueberries, cranberries and blackberries ringgon are examples of suitable back shinny Titanium species of the present invention. Blackcurrants, redcurrants, whitecurrants, greencurrants and gooseberries are examples of ribose species suitable for the present invention. Examples of suitable in the present invention gave Peru's species, it may be mentioned juniper (dusong).

Cloudberry is a useful wild berry with a high aroma content and also contains useful seed oil. Although cloudberries are used in the food, liqueur and cosmetic industries, they currently only use seed oil from the waste remaining after pressurizing the berries. The ellagic acid content in cloudberries is 0.6 mg per 1 g dry weight of berry fruit (fruit + seeds). The ellagitannin content in cloudberries is 24.6 mg per 1 g dry weight of berry fruit.

Arc bramble has a very high content of ellagic acid and ellagitannin, making it very suitable as a raw material for the process of the present invention. From an ecological point of view, wild berry such as cloud berry and arc scramble, grown without artificial fertilizers or pesticides, are particularly suitable.

fruit

Fruit varieties of Vitis, Funica, Pyrus, Malus, Citrullus, Beninkasa, Cucumis, Momordica, Olea and Argania spinosa species are suitable for the method of the present invention. All grapes and all grape hybrids containing seeds and belonging to the Vitis genus can be used in the present invention. Typically, grapes also provide a raw material source, which is particularly suitable for the present invention, because enormous amounts of waste are obtained by processing the vines, such as by pressing the vines.

Pomegranate (Nika Fu Gras natum), pear (pie Russ communication bus) and an apple tree is (the end of the Russ), and the fruit is also suitable sources of these raw materials of fruit trees, like all hybrids. A significant amount of waste is obtained in the processing of these fruits.

Keeping plants

Species of the genus selected from turnip rapeseed ( brassica rapa ), sunflower, flax, hemp ( cannabis sativa ) and rapeseed ( brassica napus ), and all hybrids thereof, are suitable for the method of the invention.

Berry materials, fruit materials and oil and vegetable material suitable for the method of the present invention may be selected from seeds, leaves, berry, fruits, and by-products, side streams and waste resulting from the processing of the berry, fruit or oil or vegetable. Examples of such by-products, side streams and wastes include compressed cakes, debris, berry cakes, fruit cakes and fractional residues. The by-products, side streams and waste generally include berry or fruit rinds or husks, seeds, pulp, leaves, trees and coniferous leaves, but this also depends on how well the berry or fruit or seeds are mechanically washed before processing. .

Processing of berry, fruit or fat plants is carried out, for example, in equipment for processing or refining or fractionating berry, fruit or fat plants, or for drinks, pastes, purees, wines, jams, conserves, candies, etc. It can be carried out at a facility in the food or feed processing industry to manufacture. Particularly preferably, the present invention uses by-products, side streams and wastes.

Generally, in a juice making line, the berry or fruit may be compressed and the remaining compressed cake may be stored frozen at about −20 ° C. for later use, or otherwise dried.

Berry and fruit pastes and purees may be obtained by pressing the berry or fruit, for example through a sieve, and freezing the remaining berry cake or fruit cake at about −20 ° C., or otherwise drying it. The frozen compacts, berry cakes or fruit cakes obtained may contain 40 to 70% by weight of water, usually 50 to 60% by weight of water.

A by-product, or side stream of waste, for example, compressed cake or compressed waste obtained from grape berry rubeoseu or non-tooth in the inside it may mainly contain the seed, pericarp, or rind, and some of the pulp.

Seeds may include small amounts of rind, skin and pulp.

fair

The present invention relates to a method for converting a plant material, wherein the method mills at least one plant material in the first step, in the presence or absence of the first medium, from 1 to 5 times, with an average particle size of 10 to 1000. To obtain particles which are μm;

In a second step, the particles are mixed with the second medium at a weight ratio of 1: 3 to 1:80 at a temperature of 10 to 120 ° C. for 20 minutes to 20 hours to obtain a mixture including the bioactive compound, and the mixture Separating the plant extract from the plant residue by separating it.

The present invention particularly relates to a method for converting plant material into plant extracts having antimicrobial activity.

Preferably the plant material is rubeoseu, Mysore bus, Rosa, M. Petrov room, Chokeberry and Hippo pie in, and Mr. derived from the berry or fruit or keeping plants are selected from a combination of both, leaves, fruits, compressed waste, compressed Selected from cakes, precipitates and combinations thereof, the fruits are Vitis, Funica, Pyrus, Malus, Citrullus, Beninkasa, Cucumis, Momordica, Olea and Argania Spinosa species , And combinations thereof. Particular preference is given to using plant materials derived from raspberries.

First step

In a first step, at least one plant material is ground 1 to 5 times in the presence or absence of a first medium to obtain particles having an average particle size of 10 to 1000 μm.

The milling can be carried out in the presence of a first medium or without a medium. In one embodiment, at least the first milling can be carried out without the first medium.

The first medium may be selected from water, ethanol, ethyl acetate, acetone, methylethyl ketone, glycerol and combinations thereof. When preparing food grade products, water or ethanol is preferably used.

Grinding (or milling) in the presence of the first medium is carried out in millstone mills such as colloid mills or ultrafine mills. The milling is carried out once to five times, preferably two to four times in the presence of the medium, to obtain a milled suspension. In one embodiment, the milling is carried out two to three times. Especially preferably water is used as the first medium (wet grinding). Optionally, the plant material may be pre-milled using a homogenizer, twin screw refiner or coarse mill. In the case of using pre-crushing, it is often sufficient to pass through the mill mill once.

In one embodiment, pulverization (or milling) (dry milling) without media is used for fine impact mills, jet mills, mortar mills, disk mills, rotor mills, hammer mills, ultrafast centrifugal mills. And a mill or mill selected from the group consisting of separation mills, turborotor mills, classifier mills, and combinations thereof.

Examples of such mills include the Masuko Ceren mill DAU, the Netzsch CGS fluid bed jet mill and the Hosokawa Alpine micro impact mill Ultraplex UPZ. The material may be pre-milled using, for example, a cutting mill, a hammer mill or a disk mill. It is also possible to carry out drying at the same time as grinding, for example using a Goergens turborotor mill.

Dry grinding is carried out once to five times and at various rotor speeds. The grinding parameters are set in accordance with the instructions provided by the mill manufacturer.

In milling in the presence of the first medium, the medium, suitably water, is added to the plant material to obtain a dry content of 3-30% by weight, preferably 5-25% by weight in the milled suspension. The plant material may comprise the first medium (water) in an amount that does not require additional medium. The first medium provides effective grinding. The grinding devices are adjusted according to the instructions provided by the mill manufacturer.

From the milling in the presence of the first medium, a homogeneous gel or suspension is obtained comprising particles having an average particle size of 10 to 1000 μm. Suitably, the average particle size distribution by laser diffraction is D50 = 10-200 μm and D90 = 100-1000 μm.

The resulting grinding suspension comprising the particles remains homogeneous even when heated. Thus, after grinding, the suspension can be frozen or used directly in the second step without the risk of phase separation.

2nd step

In a second step, the particles obtained in the first step are mixed with the second medium in a weight ratio of 1: 3 to 1:80 for 20 minutes to 20 hours at a temperature of 10 to 120 ℃ to obtain a mixture, the mixture Separating the plant extract from the plant residues by separating it.

The second medium is selected from water, ethanol, ethyl acetate, acetone and combinations thereof. When preparing food grade products, water or ethanol is used. Preferably water is used, in which case the second step may be called hot water extraction.

In one embodiment, in the second step, the weight ratio of the particles (or suspension) obtained in the first medium to the first step carried out in the presence of the second medium is each from 1: 3 to 1:80, preferably 1: 5 to 1:50. In a preferred embodiment, in the second step, the particles are mixed with the second medium in a weight ratio of 1:10 to 1:30.

In one embodiment, in the second step, the ratio of the particles (or suspensions) obtained in the first medium to the first step carried out in the presence of the second medium is from 1: 5 to 1:40, respectively.

In one embodiment, the particles or suspension obtained in the first step are mixed with the aqueous medium, usually using a mixing speed of 100-1500 rpm. Any suitable mixing device can be used.

In one embodiment said second step is carried out in the presence of an acid. The acid is selected from ascorbic acid, oxalic acid, citric acid, acetic acid, malic acid, benzoic acid and HCl, preferably ascorbic acid is used. The concentration of acid in the second medium is from 0.01 to 0.2% by weight, preferably from 0.05 to 0.1% by weight. Preferably, the second medium is water and the acid is ascorbic acid. Weak acid conditions have a stabilizing effect on anthocyanins.

In one embodiment, in the second step, the mixing temperature may range from 20 to 95 ° C, preferably 25 to 90 ° C.

In one embodiment, in the second step, the mixing may be carried out in at least two mixing steps, in which case the temperature in the first mixing step is 10-50 ° C., preferably 30-47 ° C., and the second mixing The temperature in the step is 50-120 ° C., preferably 60-95 ° C.

In one embodiment, the mixing is carried out under a pressure of 1 to 10 bar, preferably 1 to 2 bar.

In one embodiment, when the mixing is carried out in at least two mixing stages, the mixing time in the first mixing stage is 30 minutes-12 hours, preferably 1-4 hours, and the mixing time in the second mixing stage Is 30 minutes-12 hours, preferably 30 minutes-7 hours.

Optionally, enzymes may also be used in the second step of the method.

In one embodiment, in a second step, at least one enzyme can be added to the aqueous medium. Preferably the second medium is water. The enzyme is suitably selected from carbohydrate hydrolases, suitably selected from cellulase, pectinase, xylanase and combinations thereof. The enzymes are administered based on their main activity (eg 100 to 200 nkat / g or 0.01 to 1% by weight). The enzyme is diluted in water before mixing with the aqueous medium. Depending on the enzyme, the treatment is carried out at the pH that is optimal for each enzyme or at the inherent pH of the material used. When using the enzyme, the second step is carried out for 1 to 4 hours at a temperature of 40 to 47 ℃, then for 30 minutes to 7 hours at 50 to 90 ℃.

In the second step, separation of plant extracts from plant residues (solid materials) is carried out using suitable methods such as centrifugation, filtration, decanting and the like.

In one embodiment, the plant extract obtained in the second step can be used as is in the manufacture of the product, or it can be concentrated, frozen or dried. Concentration can be carried out by vacuum evaporation, pressure filtration, or using decanter centrifugation.

In one embodiment, the plant extract obtained in the second step is dried. Drying may be performed by freeze drying, spray drying, flash drying under elevated temperature, or by a drying cabinet. Suitably, drying is carried out at a temperature of 40 to 150 ° C., and spray drying is preferably carried out at 80 to 95 ° C. The second medium obtained in the drying step can be recycled to the first or second step of the process.

Pretreatment of Plant Materials

In one embodiment of the invention, the plant material is pretreated prior to the present process. The pretreatment may be performed by adding a method selected from heat treatment, fermentation, enzymatic treatment, extraction with ethanol, pressurization, compression, crushing, and combinations thereof to plant materials.

In one embodiment of the invention, the plant material is heat treated to remove harmful microorganisms. Suitably, the heat treatment is performed at 75-90 ° C. for 5-10 minutes.

In one embodiment of the invention, the plant material is fermented using lactic acid bacteria or yeast to modify the phenolic compounds and carbohydrate components of the seed. Preferably, the seed culture is selected from Lactococcus, Lactobacillus, Pediococcus, Oenococcus genus and natural yeast derived from plant materials used for the fermentation. .

During fermentation, frozen plant material and water, suitably ultrapure water, are usually mixed together (1: 1) and suitably heated at 80 ° C. for 5 minutes. The mixture is suitably cooled in an ice bath and crushed the berry or fruit material if necessary. The pH of the mixture is suitably adjusted to about pH 5.0 with 5N sodium hydroxide. Microorganisms are pre-grown in food grade media. Fermentation is carried out in a bioreactor (container) or the like, for example, with continuous mixing at 30 ° C for 3 days. Lactic acid bacteria fermentation is purged with sterile nitrogen gas to create anaerobic conditions.

In one embodiment of the invention, the plant material is pretreated with carbohydrate hydrolase. The plant material is pressurized or compressed after enzyme incubation. Suitably the enzyme is selected from cellulase, pectinase, xylanase and combinations thereof.

In enzyme treatment, the enzymes are administered based on their main activity (such as 100 to 200 nkat / g or 0.01 to 1% by weight). The thawed and crushed (40-45 ° C.) heated plant material is incubated at 40-45 ° C. for 2-4 hours. The enzyme is diluted in water and then mixed with the crushed and heated plant material. The treatment is carried out at the intrinsic pH (about pH 3) of the material used. After enzyme incubation, the juice is extracted by a juicer. Enzyme treatment generally increases the yield of plant extracts and the compounds contained therein.

In one embodiment of the invention, the plant material is dried until the water content is no greater than 15% by weight, preferably 0.1-10% by weight, in order to remove excess water prior to introduction into the process. The drying may be carried out by convection drying, such as hot air drying, vacuum drying or steam drying, heat drying, microwave drying with or without vacuum drying, or freeze drying. The drying may be carried out at a temperature of 35 to 70 ℃, preferably 35 to 45 ℃ using a fluid bed dryer. Suitably, freeze drying is carried out at a temperature of -40 to 0 ° C. and convection drying is carried out at a temperature of 40 to 70 ° C., preferably 40 to 50 ° C. Any conventional drying apparatus suitable for drying can be used.

In one embodiment, the plant material is crushed prior to processing, suitably using compressive crushing, to separate chunks of rinds, leaves, bark, pulp, etc. from the seeds and to minimize damage to the seeds. The crusher may be selected from a roll crusher, a ball crusher, a manual crusher, a kneading mill or a combination thereof. A kneading mill can be used as a shredder, which separates the dry pulp and rind portion without breaking the berry seeds. Suitable mills are falling number mills comprising a rotary rotor and a stationary stator. This mill provides a kneading effect and a light cutting and striking effect, in which the grinding energy is separated from the substrate, although the grinding energy is not sufficient to destroy the seeds. It is preferable to use a disk mill or an impact mill using guided impact, which results in a smoother grinding process.

The plant extracts obtained by the process of the present invention (extracts contained in the second medium or dry extract) are rich in phenolic compounds such as ellagic acid, ellagitannin and their derivatives and other bioactive compounds, and also the C18 fatty acids. It may contain.

The plant extracts can be used in cosmetics and skin care products such as masks and exfoliating creams, hygiene products, household products, machine parts such as membranes and filters, dietary supplements, foodstuffs, food supplements, animal feed, pet food, packaged foods. And as natural preservatives in pharmaceuticals.

Plant extracts possessing antimicrobial activity obtained by the method of the present invention, as can be seen from the examples, showed a particularly excellent effect against Staphylococcus aureus, it was also effective against Pseudomonas aeruginosa and E. coli.

In skin care products and hygiene products, plant extracts can be used to stabilize, balance, and protect healthy skin microbiota, which allows these fractions to grow in large numbers of pathogenic microorganisms without affecting the growth of beneficial microorganisms. This is because it suppresses.

The plant extracts obtained by the method of the present invention may be used in foodstuffs, cosmetics, hygiene products, household products, pharmaceutical products, machine parts, nutraceuticals, foodstuffs, food supplements, animal feeds, pet food, packaging materials, particularly easily spoiled foods. Properly incorporated into food packaging. Examples of such products include topical preparations such as creams, ointments, skin care products, diapers, feminine hygiene pads, tampons, incontinence pads and diapers, and the like.

Examples of rapidly depleting foodstuffs include poultry products, marinades, dairy products such as yoghurt, alcoholic beverages, sour cream products, fermented milk products; Jams, beverages, berry soups, conserves, pastes, purees, baby foods; Nutritional products, particularly for special purposes such as hospital use and hose administration; Cereal products such as bread, cereals, confectionary, muesli, precooked porridge, grain fermented products and gluten-free products.

Plant residues generally contain large amounts of ellagic acid (approximately 80 g / kg), C18 fatty acids, fiber and lignin, so they have very good additives, especially for nutraceuticals, foodstuffs, pet foods, food supplements and feeds. to provide.

The present invention provides several advantages. Seeds, leaves, by-products, side streams and wastes originating from the industry of processing plant materials such as berry, fruit and oil and fat plants are effectively used in the simple and economical process of the present invention for obtaining plant extracts having antimicrobial activity. Can be. The leaves can be harvested separately or simultaneously with the berry or fruit.

Plant extracts possessing antimicrobial activity can be used as effective antioxidants, antimicrobials and preservatives.

Dumping waste into landfills can be prevented or at least significantly reduced. This is also a clear environmental and ecological benefit. Nutrient-rich and beneficial wastes and by-products from the plant processing industry can be used in a simple and efficient manner in packaged foods, cosmetics and pharmaceuticals, as well as in a variety of products such as food and animal feed.

The antimicrobial effect of plant extracts is dose dependent.

The present invention provides the product with improved storage time and microbiological safety, since the plant extracts possessing antimicrobial activity act as preservatives. It is possible to reduce the amount of synthetic preservatives in the product and replace them with these natural compounds. In addition, in cosmetics, these natural compounds effectively inhibit the growth of skin pathogens such as staphylococci, thus correcting the balance of the skin microbiota.

The present invention provides for improved microbiological preservation, improved inhibition of oxidation reactions and increased antioxidant status for the products into which they are introduced. In general, this means that stability and microbiological safety have been improved. Plant extracts are readily soluble in water, which is an important technical advantage. Plant extracts contain no free sugars or only in very limited amounts, improving the stability of the product. Thus, plant extracts are not sticky and are easily stored in frozen form. The entire procedure for preparing plant extracts can be carried out in an edible grade process, and toxic or hazardous reagents or process steps are inevitable. Thus, the extract is also particularly suitable for food use.

Example

The following examples illustrate the embodiments of the invention described above and do not limit the invention in any way.

Example 1

Wet Grinding, Hot Water Extraction and Antimicrobial Activity of Berry Compressed Cakes

Frozen berry compacts obtained from bilberry (100%), bilberry (50%) and lingonberry (50%), cloudberry (100%) and raspberry (100%) in the berry processing industry. Wet grinding in quarters. The test settings are shown in Table 1. The compressed residue was combined with water to obtain a slurry having a dry content of 25-40% by weight depending on the initial raw material. Water was used to improve throughput in the mill. First, the cloudberry compressed residue was ground without dilution to obtain a soft paste. The grinding was performed at 1500 rpm and various gap widths of 0.3 to 1.5 mm. Grinding millstones were millstones of type MKE10-46 made of silicon carbide and resin having a diameter of 10 ". Millstones made of aluminum oxide may also be used. The quality of the material is that the grinding millstone is moved by moving the lower millstone. The compression residues were subjected to compressive and shear forces between the millstones to determine the particle size of the material from which they were produced.The average particle size distribution of the suspension obtained was one pass through the millstone, 2 Measurements were made after one pass and three passes: D50 of the product after one pass was about 270 μm, but finer D50 = 29 μm after three passes.

The suspension was freeze dried or used as is for hot water extraction.

For hot water extraction, water was added to 50 g of berry suspension (freeze dried or used as such) to obtain a final dry weight content of 1 g of dry material per 20 ml of water. Extraction was performed at 80 ° C. (with mixing at 500 rpm) for 1 hour. The mixture was filtered using a Miracloth® filter material of 22-25 μm pore size to obtain a filtrate and a residue. The filtrate was freeze dried.

The antimicrobial activity of the freeze dried filtrate (berry materials) and combinations thereof was tested against selected microorganisms including Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa.

Antimicrobial activity was measured in liquid culture. 2.5 mg / ml of lyophilized material was suspended in microbial culture. A microbial culture without berry material was used as a positive control, and a culture containing antibiotic (chloramphenicol) was used as a negative growth control. The microbial cultures were incubated with optimal growth conditions, and growth by cell number was confirmed by plate count during the 24-hour incubation process.

As a result, it was shown that there was an obvious antimicrobial activity (about 2 log units) against Staphylococcus aureus of the hydrolyzed extract of the wet milled berry fraction compared to the positive control culture containing no antimicrobial agent. Raspberry was the best inhibitor. Moderate growth inhibition was also seen in E. coli. Cloudberry was the best inhibitor. There was only a slight inhibition of Pseudomonas aeruginosa . Raspberry was the best inhibitor. Good antimicrobial activity was associated with samples not freeze dried prior to hydrothermal extraction.

Figure 1 shows the antimicrobial effect of Staphylococcus aureus of hydrothermal extracts of wet milled compressed cakes during 24 hours incubation in liquid culture. Cultures containing the antibiotic chloramphenicol (50 μg / ml) were used as negative controls. The effect of the extract was tested in one concentration, 2.5 mg / ml culture. The effect of E. coli of the same extract is shown in FIG.

Example 2

Wet grinding, hydrothermal extraction and antimicrobial activity of berry compressed cakes from the berry liqueur manufacturing industry

Cloudberry press residue from Liqueur was wet milled in a Masco ultrafine mill mill (model MKZA10-15J). The compressed residue was obtained from two preparation batches after a short (4 days) and a long (4 months) ethanol extraction period. The first batch was 400 g and the second batch was 1 kg. First, the compressed residue was ground without dilution to obtain a soft paste. According to the moisture content analyzer, the dry matter content was 30-34%. The gap width between the grinding millstones was 0.8 mm. For the second pass, the compacted ground debris was combined with water to give a slurry of 10% solids. In the second pass, the gap width was 0.3 mm. The grinding was carried out at 1500 rpm. The grinding mill was a millstone of type MKGA10-46 made of aluminum oxide and resin having a diameter of 10 ". The quality of the material was controlled by moving the lower millstone to set the spacing between the grinding millstones. Compressive and shear forces between millstones were applied to determine the particle size and homogeneity of the suspension, the material from which it was produced, which was either freeze-dried or used for hot water extraction.

For hot water extraction, water was added to 50 g of berry suspension (freeze dried or used as such) to obtain a final dry weight content of 1 g of dry material per 20 ml of water. Extraction was performed at 80 ° C. (with mixing at 500 rpm) for 1 hour. The mixture was filtered using Miracloth® filter material having a pore size of 22-25 μm. The filtrate was freeze dried.

The antibacterial activity of the freeze dried filtrate (berry fraction) against selected microorganisms, including Staphylococcus aureus and Pseudomonas aeruginosa , was tested as described in Example 1 except that the concentration tested was 5 mg / ml.

As a result, it was shown that compared to the positive control culture containing no antimicrobial agent, the hydrothermal extract of the wet milled berry fraction has a strong antibacterial activity against Staphylococcus aureus. Compression residues subjected to short-term ethanol extraction were better inhibitors than compression residues subjected to long-term extraction. Strong inhibition was also shown for Pseudomonas aeruginosa . In this case, good antimicrobial activity was associated with samples subjected to prolonged ethanol extraction before the wet milling process.

Figure 3 shows the antimicrobial effect of Staphylococcus aureus of hydrothermal extracts of wet milled compressed cakes during 24 hours incubation in liquid culture. Cultures containing the antibiotic chloramphenicol (50 μg / ml) were used as negative controls. The effect of the extract was tested in one concentration, ie 5 mg / ml culture. The effect of the same extract on Pseudomonas aeruginosa is shown in FIG. 4.

Example 3

Dry Milling, Hot Water Extraction and Antibacterial Activity of Raspberry Leaves

Fresh wild raspberry leaves were collected from nature after berry harvest (September) and frozen. Frozen leaves were lyophilized and milled at 29 Hz using a ball mill (Retsch).

For (hot water) extraction, 200 ml of water was added to 10 g of leaf powder. Extraction was performed at 80 ° C. (with mixing at 500 rpm) for 1 hour. The mixture was filtered using Miracloth® filter material having a pore size of 22-25 μm. The filtrate was freeze dried.

The phenolic profile was measured from 100 mg freeze dried samples extracted by homogenization with 1 ml of methanol. The samples were run using Waters Alliance HPLC-DAD (High Performance Liquid Chromatography-Photodiode Array Detection) with Hypersil BDS-C18 columns (4.6 × 150 mm, 5 μm, 25 ° C.). I was. The solvent was 5% formic acid (A) and acetonitrile (B) and the gradient was 5% B to 90% B for 35 minutes at a flow rate of 0.700 ml / min. Injection volume was 20 μl and data were collected in the 190-600 nm range. Ellagic acid and ellagitannin were detected according to their specific spectra.

HPLC chromatograms of hydrothermal extracts of raspberry leaf material were rich in ellagic acid and ellagitannin. HPLC chromatograms of hydrothermal extracts of raspberry leaves are shown in FIG. 5 and UV absorption spectra of the main compounds are shown in FIG. 6. Peaks 1 and 2 are typical peaks for ellagitannin and peak 3 are typical peaks for ellagic acid.

The antimicrobial activity of the freeze dried leaf material was tested against selected microorganisms, including Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa.

Antimicrobial activity was measured in liquid culture. 1, 2.5 and 5 mg / ml of lyophilized material were suspended in microbial culture. A microbial culture without berry material was used as a positive control, and a culture containing antibiotic (chloramphenicol) was used as a negative growth control. The microbial cultures were incubated with optimal growth conditions and growth was confirmed by plate counting after cell counting for 24 hours of incubation.

As a result, compared to the positive control culture containing no antimicrobial agent, the hot water extract of the crushed berry leaf fraction showed a very strong antibacterial activity against Staphylococcus aureus. It was clear that the inhibition was dependent on the concentration of the extract. The highest concentration of 5 mg / ml completely eliminated bacteria within 7 hours of incubation. Weak growth inhibition was shown for E. coli and P. aeruginosa .

Figure 7 shows the antimicrobial effect of Staphylococcus aureus of the hydrothermal extract of raspberry leaves milled during 24 hours incubation in liquid culture. Cultures containing the antibiotic chloramphenicol (50 μg / ml) were used as negative controls. The effect of the extract was tested in three concentrations, 1, 2.5 and 5 mg / ml culture.

Example 4

Pretreatment of Berry Ingredients: Fermentation, Pressurization and Sieving of Cloudberries

Fermentation

Ripe frozen cloudberries (Rubus chamaemorus) were used as the berry material. The berry material was first heat treated and then inoculated with about 10 ⁶ cfu / g of washed LAB cells. Pedicoccus pentosaceus VTT E-072742 from the VTT Culture Collection was used as seed culture for cloudberry fermentation (http://culturecollection.vtt.fi/). Prior to fermentation, the strain was regenerated for one day in de Man Rogosa Sharpe medium under 100% carbon dioxide atmosphere made using anaerobic jars and Anaerocult C strips. The cells were collected from the regenerated culture by centrifugation and washed once in Ringer's solution. Fermentation was carried out on a scale of 6 kg with continuous mixing (130 rpm) at 30 ° C. for 3 days in a 15 liter bioreactor. The bioreactor was purged with sterile filtered nitrogen gas to create anaerobic conditions. Viable counts of lactic acid bacteria and yeast before and after fermentation were measured by plate counting technique. The results are expressed in colony forming units (CFU) per 1 g wet weight. Fermented mashed berry was stored frozen.

Pressurized and dry

After fermentation, the crushed berry was treated with a hydraulically operated high pressure tincture press using 5 liters of filler to separate the juice and insoluble compressed cake.

The compressed cake separated from the juice press was dried in a fluid bed dryer using a + 45 ° C. air stream until the berry compressed cake had a water content of less than 15% by weight. The dried berry compacted cake was then dry sieved using different sieve sizes or using a suction device. Peel fractions of average particle size less than 1250 μm were separated and seed fractions of average particle size of 750 μm or more were collected.

The mass yield of cloudberry compressed cakes (fermented and unfermented samples) was 8-10%. About 5% of the cakes consisted of shells and pulp and the remaining 95% were seeds. The compressed cake was either used as is in the process or frozen for later use.

Example 5

Composition and Sensory Properties of Hydrothermal Extraction Residues of Wet Crushed Berry Compressed Cakes

Hot water extraction was performed by wet or dry milling of the berry sidestream, ie the berry compacts from the berry processing industry, or the berry leaves described in Example 3. The composition of the residue is 1) fiber, protein and dry weight; 2) phenolic compounds, fatty acids and other fat soluble compounds and 3) organoleptic properties.

Fiber, protein and dry weight

The dry matter content of the hydrothermal extraction residue was analyzed using the oven method, and the protein content was analyzed by the Kjeldahl method. Total dietary fiber, soluble and insoluble dietary fiber were analyzed using the enzyme gravimetric method (AOAC Official method 991.43).

In the residue of the wet milled side stream, the dry matter content was about 45%, whereas in the residue of dry milled raspberry leaves it was about 25% (Table 2). Most of the berry residues or more than 70% of the dry matter were insoluble fibers, and wet crushed cloudberries also had a high soluble fiber content (8% of the dry matter) (Table 3). The protein content of the berry material was 6-7% (d.m.) and over 20% in the residue of the raspberry leaf.

Phenolic Compounds, Fatty Acids and Other Fat-Soluble Compounds

The hydrothermal extraction residue was dried before analysis.

The phenolic profile was determined from 100 mg of air dried sample extracted by homogenization with 1 ml of methanol. The samples were run using Waters Alliance HPLC-DAD (High Performance Liquid Chromatography-Photodiode Array Detection) with a Hypersil BDS-C18 column (4.6 × 150 mm, 5 μm, 25 ° C.). The solvent was 5% formic acid (A) and acetonitrile (B) and the gradient was 5% B to 90% B for 35 minutes at a flow rate of 0.700 ml / min. Injection volume was 20 μl and data were collected in the 190-600 nm range. Using ellagic acid, camphorol and cyanidin-3-glucoside as external standards for calibration curves, ellagic acid and quercetin were quantified at 360 nm and anthocyanin at 520 nm.

HPLC chromatogram of extraction residue at 360 nm wavelength is shown in FIG. 8. The compressed cake residues contained some phenolic compounds, which differed from one another in different compressed cakes. The main phenolic compound in the bilberry compressed cake extraction residue was anthocyanin. Their content was 2.75 g / kg of dried extract residue. The main compound in the bilberry-lingonberry compressed cake extract residue was quercetin. The content was 130 mg per kg of dried extraction residue. The main compound in cloudberry and raspberry compressed cake residues was ellagic acid. The content was 248 mg per kg dried extract residue in cloudberry and 100 mg per kg dried extract residue in raspberry.

In conclusion, since compressed cakes contain health promoting phenolic compounds, they can be potential food ingredients. In particular, in bilberry compressed cakes, the anthocyanin content was very high on the same order scale as in the berry.

8, HPLC chromatograms of A: bilberry compressed cake residue, B: lingonberry-bilberry compressed cake residue, C: cloudberry compressed cake residue and D: raspberry compressed cake residue are shown. In the figures ellagic acid, quercetin and anthocyanin are indicated by arrows.

Fatty acids were measured using an FFAP silica capillary column on Agilent GC-MS. Gout dried samples (20 mg) were spiked with C17 triacylglycerol and free fatty acids. The sample was mixed with petroleum ether (bp 40-60 ° C.), transesterified with sodium methoxide and acidified with sodium hydrogen sulfate. Esterified free fatty acids were identified using a mixture of relevant standard materials commercially available. For the determination of sterols, tocopherols, squalene and amilines, the transesterified samples were trimethyl with N-methyl-N-trimethylsilyl) trifluoroacetamide (MSTFA) containing 1% trimethylchlorosilane (TMCS). Silylation was run by GC-MS on a DB-5MS column (30 m). The identification was based on residence time and mass spectra of the relevant standard materials and comparison with Palisade's Complete 600K mass spectral library (Palisade Mass Spectrometry, USA).

The fatty acid composition of the extraction residues is shown in Tables 4 and 5. The extraction residues were all rich in fatty acids. The highest content appeared in the raspberry compressed cake residue. Most fatty acids were in combined form (greater than 90%). The highest content of free fatty acids appeared in the bilberry compressed cake residue (about 10%). The main fatty acids in all extraction residues were oleic acid, linolenic acid and α-linolenic acid, which are also the main fatty acids in the corresponding berry seed. The fatty acid composition of the extract residues has been shown to be very beneficial for human health because the residues are rich in essential fatty acids. Other fat soluble compounds are shown in Table 5. The amounts of other fat soluble compounds analyzed by GC-MS are shown in Table 6. Amirin, plant sterols and squalene have been found in particular in lingonberry and bilberry extract residues, which are associated with cardiovascular health. Raspberry extract residues were most abundant in tocopherols. Thus, all of the extraction residues are expected to be very promising as food ingredients.

Sensory properties of the hydrothermal extraction residues were screened. The hydrothermal residue in this assessment was derived from wet milled cloudberry and raspberry seed compressives, and wet milled bilberry compressed cakes and wet milled bilberry-lingonberry mixture compressed cakes. Five to six inspectors performed a free descriptive analysis of the appearance, aroma, taste and structure. Extract residues were evaluated as having potential as a fiber rich food ingredient in the primary assessment. The results were provided as a set of descriptive attributes for the sample.

For the hydrothermal residues evaluated above, various berry-like properties were shown (Table 7). Most of the taste attributes were positive or neutral. Thus, the hydrothermal extract residue can be viewed as a promising food ingredient with the potential to enhance the nutritional properties of the food product without negatively affecting its organoleptic properties.

While the present invention has been described in connection with specific embodiments, including preferred embodiments thereof, it will be apparent to those skilled in the art that various changes and modifications can be made to the above-described aspects that fall within the spirit and scope of the invention. There will be. It is to be understood that the invention is not limited to its application to the detailed description of the composition and arrangement of the components described herein. The above modifications and variations are within the scope of the present invention.

Claims (16)

As a method of switching plant material,
The method mills at least one plant material in the first step, once to five times in the presence or absence of the first medium to obtain particles having an average particle size of 10 to 1000 μm, and in the second step The particles are mixed with the second medium at a weight ratio of 1: 3 to 1:80 at a temperature of 10 to 120 ° C. for 20 minutes to 20 hours to obtain a mixture containing the bioactive compound, and separating the mixture to maintain plant residue. Separating the plant extract from the water. The method of claim 1, wherein the plant material is seed, berry, fruit, leaf, compressed residue, compressed cake, berry cake, fruit cake; Wastes, sediments, side streams and by-products from the industry of processing plant materials; And any combination thereof. The method according to claim 1 or 2, characterized in that the plant material is selected from berry material, fruit material, oily vegetable material and combinations thereof. 4. The method of any one of claims 1 to 3, wherein the at least one plant material in the first step is the presence of a first medium selected from water, ethanol, ethyl acetate, acetone, methylethyl ketone, glycerol and combinations thereof. Pulverizing under the process. 5. The method according to claim 4, wherein in the first step at least one plant material is ground in a mill mill. 4. The method of any one of claims 1 to 3, wherein in the first step, without using a medium, a fine impact mill, a jet mill, a mortar grinder, a disk mill, a rotor mill, a hammer mill And milling in a mill selected from the group consisting of branching, ultrafast centrifugal mills, turborotor mills, classifier mills, and combinations thereof. The process according to claim 1, characterized in that in the second step the particles are mixed with a second medium selected from water, ethanol, ethyl acetate, acetone and combinations thereof. 8. The process of claim 1, wherein the second step is carried out in the presence of an acid selected from ascorbic acid, oxalic acid, citric acid, acetic acid, malic acid, benzoic acid and HCl, preferably using ascorbic acid. Characterized in that the method. The method of claim 1, wherein in the second step, the mixing is performed in at least two mixing steps, in which case the temperature in the first mixing step is 10-50 ° C., and the second The temperature in the mixing step is characterized in that 50-120 ° C. 10. The method according to claim 1, wherein the mixing is carried out in a second step under a pressure of 1 to 10 bar. 11. The method of claim 1, wherein in the second step at least one carbohydrate hydrolase is added to the second medium, preferably the enzyme is cellulase, pectinase, xylanase. And combinations thereof. The method according to any one of claims 1 to 11, characterized in that the plant extract obtained in the second step is used as is in the manufacture of the product or is concentrated, frozen or dried. The method according to claim 12, characterized in that the plant extract obtained in the second step is dried by freeze drying, spray drying, heat drying, drying at elevated temperature or drying in a drying cabinet. The method according to any one of claims 1 to 13, wherein the plant material is pretreated before the first step, and the pretreatment is performed on the plant material by heat treatment, fermentation, enzyme treatment, extraction with ethanol, pressurization, compression, drying. Characterized by performing by adding a method selected from crushing and combinations thereof. The method according to any one of claims 1 to 14, characterized in that a plant extract having antimicrobial activity is obtained. Cosmetics, skin care products, hygiene products, household products, nutraceuticals, foodstuffs, food supplements, feeds, pet foods, packaged foods, packaging materials, obtained by the method of any one of claims 1 to 15, Use in mechanical parts or pharmaceuticals.

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