KR20190072316A - Method for production for healthy vegetable cheese with soybean - Google Patents

Method for production for healthy vegetable cheese with soybean Download PDF

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KR20190072316A
KR20190072316A KR1020170173578A KR20170173578A KR20190072316A KR 20190072316 A KR20190072316 A KR 20190072316A KR 1020170173578 A KR1020170173578 A KR 1020170173578A KR 20170173578 A KR20170173578 A KR 20170173578A KR 20190072316 A KR20190072316 A KR 20190072316A
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South Korea
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cheese
soybean
lactic acid
soybean milk
whey
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KR1020170173578A
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Korean (ko)
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정성수
이순구
정연호
박점선
허문행
정일환
최승호
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주식회사 정.식품
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Priority to KR1020170173578A priority Critical patent/KR20190072316A/en
Publication of KR20190072316A publication Critical patent/KR20190072316A/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C20/00Cheese substitutes
    • A23C20/02Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates
    • A23C20/025Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates mainly containing proteins from pulses or oilseeds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C11/00Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/10Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
    • A23C11/103Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00Cheese; Cheese preparations; Making thereof
    • A23C19/02Making cheese curd
    • A23C19/04Making cheese curd characterised by the use of specific enzymes of vegetable or animal origin
    • A23L11/09
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/50Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/123Bulgaricus
    • A23Y2220/15

Abstract

The present invention relates to a production method of a vegetable cheese which can replace conventional animal cheese using vegetable material beans and, more specifically, to a vegetable cheese which can replace animal cheese by inoculating and cultivating lactic acid bacteria in soymilk to produce fermented soybean milk, and after separation of whey, and mixing raw materials enhancing sensory palatability, and to a production method thereof. The present invention provides a vegetable cheese excellent in sensory aspect and in healthy to those who avoid animal cheese or vegetarians, people on a diet due to adult diseases.

Description

콩을 이용한 식물성 건강 치즈의 제조방법 {Method for production for healthy vegetable cheese with soybean}FIELD OF THE INVENTION [0001] The present invention relates to a method for producing a healthy vegetable cheese with soybean,

본 발명은 식물성 소재인 콩을 이용한 치즈의 제조방법에 관한 것으로서, 더욱 구체적으로는 두유에 유산균을 접종하여 발효시킨 후에 유청을 분리하고 첨가물을 혼합하는 공정을 포함하는 식물성 치즈의 제조방법에 관한 것이다.The present invention relates to a method for producing cheese using soybean which is a vegetable material and more specifically to a method for producing vegetable cheese including a step of inoculating lactic acid bacteria in soybean milk and fermenting the soybean milk, .

치즈(Cheese)는 일반적으로 동물의 젖에 들어있는 단백질(카제인,casein)을 응유효소인 렌넷(rennet)으로 응고시킨 식품으로, 맛과 영양이 우수하여 많은 사람들이 이용하고 있다.Cheese is a food made by coagulating protein (casein), which is generally contained in animal milk, with rennet, a cofactor enzyme, and is used by many people because of its excellent taste and nutrition.

현재의 치즈는 원유를 살균하는 단계, 유산균(치즈스타터)과 렌넷을 첨가하여 유단백질인 카제인을 응고시켜 커드를 만드는 단계, 커드로부터 유청을 분리하고 스트레칭과 성형을 하여 모양을 만드는 단계 및 염지 및 숙성 단계를 거쳐 제조되는 것이 일반적이다. The present cheese is a step of sterilizing the crude oil, adding a lactic acid bacterium (cheese starter) and rennet to coagulate the casein, which is a milk protein, to form a curd, separating the whey from the curd and forming a shape by stretching and molding, It is common to produce them by a step.

최근 국내 식품 소비 구조가 변하면서, 치즈의 소비량이 지속적으로 증가하고 있으며, 부드러운 질감과 신선한 맛을 주는 연질치즈는 그 소비량이 점차 증가할 것으로 예상되고 있다. 하지만, 연질치즈는 다른 유제품 대비 높은 가격대를 형성하고 있어 대중화하는데 어려움이 있다. Consumption of cheese has been increasing steadily in recent years as domestic food consumption structure has changed, and consumption of soft cheese, which gives a smooth texture and fresh taste, is expected to increase gradually. However, soft cheese has a higher price than other dairy products, making it difficult to popularize it.

또한, 치즈는 우유를 기반으로 만들기 때문에 동물성 식품을 기피하거나 유당불내증을 가진 사람에게는 적합하지 않은 문제가 있으며, 특히, 노약자들은 소화 문제로 인해 치즈를 좋아하지 않는다.Also, since cheese is based on milk, it is not suitable for people who avoid animal foods or have lactose intolerance. Especially, elderly people do not like cheese because of digestive problems.

따라서, 새로운 소재를 이용하여 치즈를 개발할 필요가 있는 것이다. Therefore, it is necessary to develop cheese using new materials.

대한민국 등록특허 제10-1466366호 (등록일자:2014.11.21.)에는, 로마노 치즈의 제조방법에 관한 것으로, 원유를 표준화하는 단계; 상기 표준화된 원유를 살균하는 단계; 상기 살균된 원유에 배양균과 렌넷 및 리파아제용액을 첨가하고 교반하는 단계; 상기 교반한 후 정치하는 단계; 상기 정치 후 커드가 형성되면 커드를 절단하는 단계; 상기 커드를 절단하여 생성된 유청을 분리하는 단계; 상기 커드를 소정의 형태로 몰딩하는 단계; 상기 몰딩한 커드를 압착하는 단계; 상기 압착한 커드를 염지하는 단계; 상기 염지한 커드를 건조한 후 숙성하는 단계;를 포함하여 이루어지는 것에 대한 기술이 기재되어 있다.Korean Patent Registration No. 10-1466366 (Registration Date: Nov. 21, 2014) discloses a method for producing Romano cheese, comprising the steps of: standardizing crude oil; Sterilizing the standardized crude oil; Adding the culture broth and the rennet and lipase solution to the sterilized crude oil and stirring; Stirring and then allowing to stand; Cutting the curd when the curd is formed after the standing; Separating the resulting whey by cutting the curd; Molding the curd into a predetermined shape; Pressing the molded curd; Dipping the squeezed curd; And then drying and aging the defatted curd. 대한민국 등록특허 제10-1311994호 (등록일자:2013.09.17.)에는, 치즈제조방법에 관한 것으로, 더욱 상세하게는 치즈를 빠르고 간편하게 형성하고, 긴 숙성기간 없이도 질감이 쫀득쫀득하여 미감이 뛰어난 치즈제조방법에 대한 기술이 기재되어 있다.Korean Patent No. 10-1311994 (Registration Date: Sep. 17, 2013) relates to a method for producing cheese, more specifically, a method for quickly and easily forming cheese, and a cheese having excellent texture A description of a manufacturing method is described.

본 발명은 두유에 유산균을 접종하여 발효시켜 제조하였음에도 불구하고, 종래의 치즈와 관능적 요소 및 물리 이화학적 특성이 비슷한 두유 유래 식물성 치즈의 제조방법을 개발하여 제공하고자 한다. The present invention provides a method for producing soybean milk-derived vegetable cheese having similar sensory and physicochemical characteristics to conventional cheese, even though soybean milk is fermented by inoculating lactic acid bacteria.

본 발명은 두유에 유산균을 첨가하여 발효시켜 두유 발효물을 수득하는 단계 (a); 상기 단계 (a)에서 수득한 두유 발효물로부터 유청을 분리하여 제거하는 단계 (b); 상기 단계 (b)에서 유청이 제거된 두유 발효물에, 코코넛오일, 전분, 정제염, 정백당, 잔탄검, LBG(Locust Bean Gum)을 첨가하는 단계 (c); 를 포함하는 콩치즈의 제조방법을 제공한다. (A) adding soybean milk to lactic acid bacteria and fermenting the soybean milk to obtain a soybean fermented product; (B) separating and removing whey from the soybean milk fermentation product obtained in the step (a); (C) adding coconut oil, starch, purified water, white sugar, xanthan gum and LBG (locust bean gum) to the fermented soybean milk from which the whey has been removed in the step (b); And a method for producing the soybean cheese.

본 발명의 콩치즈의 제조방법에 있어서, 상기 단계 (a)의 두유는, 바람직하게 대두에, 대두 중량의 2~6배의 열수를 첨가하여 열수 마쇄한 후, 여과하여 비지를 분리함으로써 수득된 것이 좋다.In the method for producing soybean cheese of the present invention, the soy milk of step (a) is preferably subjected to hot water grinding by adding hot water of 2 to 6 times the weight of soybeans to soybeans, and then filtered to separate the soybean oil It is good.

본 발명의 콩치즈의 제조방법에 있어서, 상기 대두는, 바람직하게 비린내를 제거하기 위하여 열처리된 것이 좋다.In the method for producing bean cheese according to the present invention, the soybean preferably is heat-treated to remove the fish meat.

본 발명의 콩치즈의 제조방법에 있어서, 상기 단계 (a)의 유산균 발효시, 일 예로 프락토올리고당(fructooligosaccharide)을 첨가하는 것일 수 있다.In the method for producing bean cheese according to the present invention, in the fermentation of the lactic acid bacteria of step (a), for example, fructooligosaccharide may be added.

본 발명의 콩치즈의 제조방법에 있어서, 상기 단계 (a)의 유산균은, 바람직하게 락토바실러스 불가리쿠스 (Lactobacillus bulgaricus)와 스트렙토코커스 써모필러스(Streptococcus thermophilus)를 포함하는 복합 유산균 제제인 것이 좋다. In the method for producing soybean cheese of the present invention, the lactic acid bacterium of step (a) is preferably a complex lactic acid bacterial preparation comprising Lactobacillus bulgaricus and Streptococcus thermophilus .

본 발명의 콩치즈의 제조방법에 있어서, 상기 단계 (b)의 유청 분리 및 제거는, 바람직하게 두유 발효물을 면포에 담아 유청을 분리하여 제거시키는 것이 좋다. In the method for producing bean cheese according to the present invention, the whey separation and removal of the step (b) is preferably carried out by separating and removing the whey by putting the soybean milk fermented product in a cotton swab.

본 발명은 동물성 치즈를 섭취하지 못하는 사람들 혹은 채식주의자 혹은 노약자들에게 종래의 치즈와 유사한 맛과 영양을 가진 치즈를 제공할 수 있다.The present invention can provide cheeses that are similar in taste and nutrition to conventional cheeses for those who can not take animal cheese or vegetarians or elderly people.

또한, 본 발명은 식물성 콩을 주원료로 사용했기 때문에, 콩에 함유되어 있는 유용성분인 식물성단백질, 불포화지방, 이소플라본 등을 그대로 섭취할 수 있으며, 열량 및 지방함량이 종래 치즈대비 유의적으로 낮아 다이어트에 우수한 효과도 나타난다.In addition, since the present invention utilizes vegetable soybean as a main ingredient, vegetable protein, unsaturated fat, and isoflavone, which are useful ingredients contained in soybean, can be taken intact, and calories and fat content are significantly lower Excellent effects on the diet also appear.

도 1은 콩 품종별 유산균 배양 시간에 따른 pH 변화를 나타낸 것이다.
도 2는 콩 품종별 유산균 배양 시간에 따른 유산균수 변화를 나타낸 것이다.
Fig. 1 shows pH changes with time of cultivation of lactic acid bacteria according to soybean variety.
Fig. 2 shows changes in the number of lactic acid bacteria depending on the cultivation time of the lactic acid bacteria according to the soybean variety.

본 발명은 두유에 유산균을 첨가하여 발효시켜 두유 발효물을 수득하는 단계 (a); 상기 단계 (a)에서 수득한 두유 발효물로부터 유청을 분리하여 제거하는 단계 (b); 상기 단계 (b)에서 유청이 제거된 두유 발효물에, 코코넛오일, 전분, 정제염, 정백당, 잔탄검, LBG(Locust Bean Gum)을 첨가하는 단계 (c); 를 포함하는 콩치즈의 제조방법을 제공한다. (A) adding soybean milk to lactic acid bacteria and fermenting the soybean milk to obtain a soybean fermented product; (B) separating and removing whey from the soybean milk fermentation product obtained in the step (a); (C) adding coconut oil, starch, purified water, white sugar, xanthan gum and LBG (locust bean gum) to the fermented soybean milk from which the whey has been removed in the step (b); And a method for producing the soybean cheese.

이하, 본 발명의 콩치즈의 제조방법에 대해 하기에서 각 단계별로 세분화시켜 설명하고자 한다. Hereinafter, the method for producing the bean cheese of the present invention will be described in detail in each of the following steps.

<단계 (a): 두유 발효물 수득>&Lt; Step (a): Obtaining soy milk fermentation product >

본 단계는 두유에 유산균을 첨가하여 발효시켜 두유 발효물을 수득하는 과정이다. This step is a process in which a fermented soybean milk is obtained by adding lactic acid bacteria to soy milk and fermenting.

본 단계에서 사용되는 두유는 통상적인 방법에 의하여 제조된 것 또는 판매되는 것을 구매하여 사용할 수 있다. 다만, 바람직하게는 대두에 대두 중량의 2~6배 만큼 열수를 가하여 마쇄하고, 여과하여 비지를 분리함으로써 수득된 두유를 사용할 수도 있다. The soymilk used in this step can be purchased and used by those manufactured or sold by conventional methods. However, it is also possible to use soymilk obtained by adding hot water to soybeans by 2 to 6 times the weight of soybeans, crushing the soybeans, and separating the beans by filtration.

대두를 열수에 담가 열처리함으로써, 마쇄된 대두에서 발생하는 콩 비린내의 원인 물질을 생성시키는 트립신 억제제(trypsin inhibitor), 적혈구 응집소(hemagglutinin) 및 리폭시게나아제(lipoxigenase)를 불활성화할 수 있다. 이때, 상기 열처리 조건은 특별한 것으로 한정되지는 않으나, 95~100℃의 온도에서 pH 6~8의 조건으로 15~20분간 처리하는 것이 바람직하고, 보다 바람직하게는 100~300rpm의 속도로 교반하면서 수행하는 것이 좋다.Heat treatments of soybeans in hot water can inactivate trypsin inhibitors, hemagglutinins and lipoxigenases, which cause soybean malignant origin in the ground soybeans. At this time, the heat treatment conditions are not limited to particular ones, but it is preferably carried out at a temperature of 95 to 100 ° C under a condition of pH 6 to 8 for 15 to 20 minutes, more preferably at a rate of 100 to 300 rpm It is good to do.

본 단계에서는 두유에 유산균을 첨가하는데, 유산균은 당업계에서 사용되는 것을 어느 것이나 사용할 수 있으나, 바람직하게는 상용 복합유산균 스타터로 알려진 YF-L812 (Lactobacillus bulgaricus Streptococcus thermophilus의 혼합 균주, Chr. Hansen사 제조, Denmark)를 1 x 106 CFU/ml의 농도로 접종하고, 37℃에서 6시간 동안 배양하여 두유 발효물을 수득하는 것이 좋다. In this step, lactic acid bacteria are added to soybean milk. The lactic acid bacteria may be any of those used in the art, but preferably YF-L812 ( Lactobacillus bulgaricus and Streptococcus Mixed strains of thermophilus , Chr. Hansen, Denmark) is inoculated at a concentration of 1 x 10 6 CFU / ml and cultured at 37 ° C for 6 hours to obtain a soybean fermented product.

한편, 본 단계에서는 유산균 배양을 원활히 하기 위해 두유에 당을 첨가하는 것이 좋은데, 상기 당은 당업계에서 사용되는 것이라면 어느 것이나 사용할 수 있고, 일 예로 프락토올리고당을 사용할 수 있다. 더욱 바람직하게는 프락토올리고당을 1~5% (v/v)를 첨가하는 것이 좋다. On the other hand, in this step, it is preferable to add sugar to soybean milk to facilitate cultivation of lactic acid bacteria. Any sugar may be used as long as it is used in the art. For example, fructo-oligosaccharides can be used. More preferably, 1 to 5% (v / v) of fructooligosaccharide is added.

<단계 (b): 두유 발효물의 유청 분리 및 제거>&Lt; Step (b): Separation and removal of whey from fermented soybean milk &

본 단계는 상기 단계 (a)에서 수득한 두유 발효물로부터 유청을 분리하여 제거하는 과정이다. This step separates and removes whey from the soybean milk fermentation product obtained in step (a).

본 발명은 식물성 단백질을 치즈 원료로 사용하기 때문에 발효물(커드에 해당)에는 수분이 포함되어 있다. 수분은 치즈 성형에 방해를 줄 수 있으므로 제거할 필요가 있다. 수분을 제거하는 방법으로는 일 예로 면포에 싸고 틀어 넣어 압력을 가하는 방법, 원심분리를 하는 방법 등의 다양한 방법을 이용할 수 있다. 바람직하게는 수득한 두유 발효물을 면포에 싸서 냉장보관하면서 중력에 의해 수분을 제거하는 것인데, 이를 통해 최종적으로 중량 대비 40~50%(w/v)의 수분이 제거된 두유 발효물을 수득하는 것이 좋다. Since the present invention uses plant protein as a raw material for cheese, the fermentation product (corresponding to curd) contains moisture. Moisture may interfere with cheese formation and needs to be removed. As a method for removing moisture, for example, various methods such as a method of applying pressure by putting it in a cloth and putting it in a cloth, and a method of centrifuging can be used. Preferably, the obtained soybean milk fermented product is wrapped in a cotton swab and stored in a refrigerator to remove moisture by gravity, thereby finally obtaining a soybean milk fermented product having a moisture content of 40 to 50% (w / v) It is good.

<단계 (c): 콩치즈의 제조>&Lt; Step (c): Preparation of bean cheese >

본 단계는 상기 단계 (b)에서 유청이 제거된 두유 발효물에, 코코넛오일, 전분, 정제염, 정백당, 향료, 잔탄검, LBG(Locust Bean Gum)을 첨가하는 과정이다. This step is a step of adding coconut oil, starch, refined salt, whitening, perfume, xanthan gum and LBG (locust bean gum) to the fermented soybean milk in which the whey has been removed in the step (b).

코코넛오일, 전분은 치즈 식감을 향상시키기 위해 첨가하는데, 코코넛오일은 실내에서 응고시키고 버터 같은 질감을 가지고 있다. 전분은 여러 개의 포도당이 글루코시드 결합으로 결합된 다당류이며, 녹말이라고도 부른다. 본 발명에서는 일 예로 타피오카 전분을 사용할 수 있다. 타피오카 전분은 상기 카사바(cassava)의 뿌리에서 채취한 식물성 전분을 말한다. Coconut oil and starch are added to improve cheese texture, while coconut oil coagulates indoors and has a buttery texture. Starch is a polysaccharide in which several glucose are bonded by glucosidic bonds and is also called starch. In the present invention, tapioca starch may be used as an example. Tapioca starch refers to vegetable starch collected from the roots of cassava.

또한, 본 발명에서는 기호성을 향상시키기 위해 정제염, 정백당, 구연산, 향료를 첨가한다. 정제염은 정제를 반복하여 얻어진 것으로 순도가 높은 염화나트륨의 결정체로, 불순물이 제거되어 위생적인 면에서 우수하고, 맛과 향을 살리는 데 도움이 된다. 정백당은 설탕 제조 과정에서 가장 먼저 만들어지는 작은 입자의 순도 높은 흰색의 설탕으로, 광택이 있어 사탕이나 젤리, 과자 등을 만드는 데 사용되며, 깔끔한 뒷맛으로 식품 및 음료수의 제조 등에 사용된다. 구연산은 결정물과 무수물이 있으며, 결정물은 청량음료수, 혼성주, 캔디, 젤리, 잼, 빙과, 통조림 등의 산성조미료 및 식용유의 산패방지제(synergist)로서 사용하며 무수물은 분말주스, 가루발포주스, 분말셔벗, 분말 케첩, 추잉 껌 등 수분을 싫어하는 식품이나 가루식품의 산미료로 사용한다. In addition, in the present invention, purified salt, citron, citric acid and flavoring are added to improve palatability. Purified salt is obtained by repeating purification, and it is a crystal of sodium chloride with high purity. Since it is free from impurities, it is excellent in hygiene and helps to save taste and fragrance. Purple sugar is the first small white sugar of small particles of sugar produced during the process of making sugar. It is used to make candies, jelly, and confectionery. It is used for manufacturing foods and beverages with clean aftertaste. Citric acid is used as an acidic seasoning such as soft drink, hyungju, candy, jelly, jam, ice cream, canned food and as a synergist of cooking oil. Anhydrous is powdery juice, Powdered sorbet, powdered ketchup, chewing gum and other foods that do not like water or used as an acidifier in powdered foods.

본 단계에서는 조직의 안정화를 위해 잔탄검과 LBG(Locust Bean Gum)을 첨가한다. 잔탄검은 백색분말로 물에 녹고, 무미, 무취, 점성은 높고 의가소성을 나타낸다. LBG(Locust Bean Gum)은 식품의 점착성 및 점도를 증가시키고 유화안정성을 증진하며 식품의 물성 및 촉감을 향상시킨다. 식품에 증점제, 안정제, 품질개량제 등으로 사용한다.In this step, xanthan gum and LBG (Locust Bean Gum) are added to stabilize the tissue. Zanthan is a black white powder which dissolves in water and has tasteless, odorless, viscous and high plasticity. LBG (Locust Bean Gum) increases the stickiness and viscosity of food, improves emulsion stability, and improves the physical properties and texture of food. It is used as a thickener, stabilizer and quality improver in foods.

또한, 본 단계에서는 두유 발효물에 치즈의 관능 및 기호성을 높이기 위한 재료(일 예로, 치즈향)를 첨가하여 할 수도 있다. In this step, a material for enhancing the sensory and palatability of the cheese (for example, cheese flavor) may be added to the soybean milk fermentation product.

한편, 본 발명에서는 상기 단계 (c) 후, 압착을 통해 모양을 만들어 용기에 담은 후 72℃ 이상에서 15초간 살균하여 출하할 수 있다. Meanwhile, in the present invention, after the step (c), the shape is formed through compression bonding, and then the container is sterilized for 15 seconds at 72 ° C or higher.

이하, 본 발명의 내용을 하기 실시예 및 실험예를 통해 더욱 상세히 설명하고자 한다. 다만, 본 발명의 권리범위가 하기 실시예 및 실험예에만 한정되는 것은 아니고, 그와 등가의 원료적 사상의 변형까지를 포함한다.Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples. However, the scope of the present invention is not limited to the following examples and experimental examples, and includes modifications of equivalent raw materials.

[[ 실시예Example 1: 대두의  1: Soybean 열수Heat number 마쇄Polish 공정] fair]

대두를 정선하여 열처리하고 대두 중량의 3배만큼 열수를 첨가한 후, 열수 마쇄하였다. 이때, 열처리 공정은 마쇄된 대두에서 발생하는 콩 비린내의 원인물질인 트립신 억제제(trypsin inhibitor), 적혈구 응집소(hemagglutinin) 및 리폭시게나아제(lipoxigenase)의 불활성화를 위하여 수행하였다.The soybeans were selected, heat-treated, hot water was added by 3 times the weight of the soybeans, and then subjected to hot water grinding. At this time, the heat treatment process was carried out to inactivate trypsin inhibitor, hemagglutinin and lipoxigenase, which are causative substances in bean curd derived from ground soybeans.

[[ 실시예Example 2: 두유와 비지를 수득하는 공정] 2: Process for obtaining soybean milk and beji]

상기 대두 마쇄물을 열처리한 다음, 이를 여과하여 두유와 비지를 각각 분리하였다. 이때, 열처리 조건은 특별히 이에 제한되지는 않으나, 97℃의 온도에서 pH 7의 조건으로 17분간 처리함이 바람직하고, 보다 바람직하게는 200 rpm의 속도로 교반하였다.The soybean flour was heat-treated and then filtered to separate soybean oil and soybean oil. At this time, the heat treatment conditions are not particularly limited, but the treatment is preferably performed at a temperature of 97 DEG C under the condition of pH 7 for 17 minutes, more preferably 200 rpm.

[[ 실험예Experimental Example 1: 콩의 종류에 따른 관능 비교] 1: Sensory evaluation according to kinds of soybean]

본 실험에 사용한 대두 선별을 위해 부석태, 대원콩, 수입콩을 선별하여 정선 공정을 거쳐 이물질을 제거한 후, 외피를 제거하였다. 상기 이물질 및 외피가 제거된 대두를 105℃에서 5분간 열처리하고 반할한 다음, 상기 반할된 대두에 대두 중량의 2.3배의 끓는 물(100℃)을 가하여 100℃ 수준의 조건으로 마쇄하였다.For the selection of soybeans used in this experiment, marigolds, marigold beans, and imported soybeans were selected, subjected to a screening process to remove foreign matter, and then the skin was removed. The soybeans from which the foreign substances and the husks were removed were heat-treated at 105 ° C for 5 minutes, and then boiled water (100 ° C) 2.3 times the weight of soybeans was added to the prepared soybeans.

상기 대두 마쇄물을 pH 7.0, 98℃ 수준에서 200 rpm으로 20분간 교반하고, 이를 1 ㎛필터로 여과하여, 두유와 비지를 각각 수득하였다.The soybean flour was stirred at 200 rpm for 20 minutes at a pH of 7.0 and 98 ° C, and then filtered with a 1 탆 filter to obtain soybean milk and soybean oil, respectively.

상기 콩 품종별로 수득한 비지가 제거된 두유액을 냉각하여 잘 훈련된 관능검사 요원 20명을 대상으로 선호도 및 만족도에 대한 관능평가를 진행하였으며, 결과는 하기 표 1, 표 2와 같았다.The soybean milk obtained by removing the soybean milk obtained from each of the soybean varieties was cooled and the sensory evaluation on the preference and the satisfaction of 20 well-trained sensory test agents were carried out. The results are shown in Tables 1 and 2 below.

콩 품종별 관능 선호도 평가 결과Sensory evaluation of soybean varieties 부석태Boo 대원콩Daewon bean 수입콩Imported beans 37a 37 a 40a 40 a 43a 43 a

(Basker에 의한 순위법 유의성 검정이며, 유의수준 5%로 신뢰수준 95%임.)(Basker's statistical significance test, with a significance level of 5% and a confidence level of 95%.)

콩 품종별 관능 만족도 평가 결과Sensory Satisfaction Evaluation Results by Soybean Variety 품종kind T.BT.B T.PT.P MM T.NT.N 부석태Boo 5%5% 45%45% 3.50 ±0.613.50 + - 0.61 0%0% 대원콩Daewon bean 5%5% 40%40% 3.25 ±0.85 3.25 ± 0.85 20%20% 수입콩Imported beans 0%0% 25%25% 3.10 ±0.643.10 ± 0.64 15%15% T.B : top box로 매우좋다(5점척도 기준) 5점 응답률
T.P : top positive로 매우좋다(5점)+좋다(4점) 응답률
M : mean으로 평균의미
T.N : top negative로 싫다(2점)+매우싫다(1점) 응답률
TB: very good as top box (based on 5 point scale) 5 point response rate
TP: very good as top positive (5 points) + good (4 points) Response rate
M: Mean means mean
TN: I do not like top negative (2 points) + I hate it very much (1 point) Response rate

실험결과, 콩 품종별 관능 선호도는 상기 표 1과 같이, 부석태가 가장 우수한 것으로 평가되었으며, 관능 만족도 역시 부석태가 가장 만족도가 높은 것으로 평가되었다. As a result of the experiment, the sensory preference of the soybean varieties was evaluated as having the best satisfaction with the sensory satisfaction as shown in Table 1, and the sensory satisfaction was also the highest satisfaction with the sensory satisfaction.

[[ 실시예Example 3: 두유 발효] 3: soy milk fermentation]

상기 실시예 2에서 수득한 두유를 40℃로 냉각하였고, 프락토올리고당 3% (v/v)를 첨가한 다음, 상용 복합유산균 스타터 YF-L812(Lactobacillus bulgaricus and Streptococcus thermophilus, Chr. Hansen, Denmark)를 1 x 106 CFU/ml의 농도로 접종하였고, 37℃에서 6시간 동안 배양하여 두유 발효물을 수득하였다.The soymilk obtained in Example 2 was cooled to 40 ° C, and 3% (v / v) of fructooligosaccharide was added thereto. Then, the commercial mixed lactic acid bacteria starter YF-L812 ( Lactobacillus bulgaricus and Streptococcus thermophilus , Chr. Hansen, Denmark) was inoculated at a concentration of 1 x 10 6 CFU / ml and cultured at 37 ° C for 6 hours to obtain a soybean fermented product.

[실험예 2: 상기 실시예 및 비교예의 두유 발효물 제조의 pH 및 유산균수 측정][Experimental Example 2: Measurement of the pH and the number of lactic acid bacteria in the soybean milk fermentation products of the above Examples and Comparative Examples]

본 실험에서는 수득한 두유액에 당원과 유산균 접종 후, 일반적으로 치즈 제조시 산보정하는 pH 5.2를 목표로 1시간 단위로 샘플링하여 pH와 유산균수를 측정하였다.In this experiment, the pH and lactic acid bacteria count were measured by sampling the soy milk solution obtained after the inoculation of the saccharide and lactic acid bacteria, in general, at a pH of 5.2, which is set at the time of cheese production, in 1 hour.

본 실험예에서 해당 두유 발효물에 레시피는 하기 표 3과 같았다.The recipe for the soybean milk fermented product in this Experimental Example was as shown in Table 3 below.

두유 발효물 레시피(%)Soy milk fermented food recipe (%) 원재료명Ingredients 배합비(wt/wt, %)Compounding ratio (wt / wt,%) 두유액Soy milk 96.994796,997 당 (프락토올리고당)Sugar (fructooligosaccharide) 3.03.0 유산균 (YF-L812) 1) Lactic acid bacteria (YF-L812) 1) 0.00530.0053 합계Sum 100100 1) YF-L812: 락토바실러스 불가리쿠스 (Lactobacillus bulgaricus)와 스트렙토코커스 써모필러스(Streptococcus thermophilus)를 포함하는 복합 유산균 제제1) YF-L812: Lactobacillus bulgaricus ( Lactobacillus bulgaricus ) and Streptococcus thermophilus ( Lactobacillus bulgaricus )

상기 실시예 및 비교예 두유액에 유산균을 접종하여 시간별로 pH와 유산균수를 측정한 결과는 도 1 및 도 2와 같았다.The results of measuring the pH and the number of lactic acid bacteria by inoculating the lactic acid bacteria into the soybean milk of the above Examples and Comparative Examples were shown in FIGS. 1 and 2.

실험결과, 도 1 내지 도 2에서 보듯이, 콩 품종별로 유산균 배양시 pH와 유산균수의 차이는 미미한 수준으로 확인하였다. 콩 품종별로 유산균 배양시, pH 5.2로 변화하기까지, 소요된 배양시간은 6시간으로 확인하였으며, 최종적으로 관능이 가장 우수하다고 평가된 부석태 두유 발효액을 치즈 제조에 사용하였다.As a result, as shown in FIGS. 1 and 2, the difference in the pH and the number of lactic acid bacteria in the cultivation of lactic acid bacteria was found to be insignificant. The cultivation time of lactic acid bacteria was changed to pH 5.2, and the incubation time was 6 hours. The fermented soybean milk fermented in soy sauce was evaluated to have the highest sensory value.

[[ 실시예Example 4: 두유  4: soy milk 발효물의Fermented 유청Whey 분리] detach]

우유를 사용하는 종래의 치즈는 커드 형성 후, 커드 내에 있는 유청(whey)을 제거하기 위해 절단 후, 가열 및 반죽하여 유청을 제거하고 치즈의 조직을 단단하게 한다.Conventional cheeses using milk are formed after curd formation, after cutting to remove the whey in the curd, heating and kneading to remove the whey and make the cheese firm.

본 발명에서는 식물성 단백질을 유산균으로 발효하여 응고시킨 후, 치즈 원료로 사용하기 때문에 발효물(커드에 해당)에는 수분이 포함되어 있어 치즈 성형에 방해를 줄 수 있으므로 제거해주었다. In the present invention, since the vegetable protein is fermented by lactic acid bacteria to be coagulated and then used as a cheese raw material, the fermented product (corresponding to the curd) contains moisture, which may interfere with the cheese molding.

수분을 제거하는 방법으로는 면포에 싸고 틀어 넣어 압력을 가하는 방법, 원심분리를 하는 방법 등의 다양한 방법을 이용할 수 있다. 본 실시예에서는 상기 실시예 3에서 수득한 두유 발효물을 면포에 싸서 냉장보관하면서 중력에 의해 수분을 제거하였으며, 최종적으로 중량 대비 40 내지 50%(w/v)의 수분이 제거된 두유 발효물을 수득하였다.As a method for removing water, various methods such as a method of putting it in a cotton cloth and putting it in a pressure and a method of centrifuging can be used. In this Example, the soybean milk fermentation product obtained in Example 3 was wrapped in cotton cloth and stored in a refrigerator while being water-removed by gravity. Finally, 40 to 50% (w / v) &Lt; / RTI &gt;

[실시예 5: 콩치즈의 제조][Example 5: Preparation of bean cheese]

수분이 제거된 상기 실시예 4에서 수득한 두유 발효물에 치즈 식감을 위해 코코넛오일, 전분을 첨가하였으며, 기호성을 향상시키기 위해 정제염, 정백당, 구연산, 향료를 첨가하였고, 조직의 안정화를 위해 잔탄검과 LBG(Locust Bean Gum)을 첨가하여 콩치즈를 제조하고자 하였다. Coconut oil and starch were added to the soymilk fermented product obtained in Example 4 in which moisture was removed. To improve the palatability, purified salt, white sugar, citric acid and flavor were added. To stabilize the tissues, And Locust Bean Gum (LBG) were added.

[실시예 6: 상기 실시예의 콩치즈 제조][Example 6: Production of bean cheese of the above example]

상기 수득한 두유 발효물에 치즈의 관능 및 기호성을 높이기 위한 재료를 첨가하여 배합비순으로 계량 및 혼합을 진행하였고 압착 및 모양을 만들어 용기에 담은 후 72℃이상에서 15초간 살균하여 제조하였다.To the obtained fermented soybean milk, ingredients for enhancing the sensory and palatability of cheese were added and metering and mixing were carried out in the order of mixing ratio, and pressed and formed into a container and sterilized at 72 ° C or higher for 15 seconds.

본 실험예에서 콩치즈의 레시피는 하기 표 4와 같았다. The recipe of the bean cheese in the present experimental example is shown in Table 4 below.

콩치즈 레시피(%)Bean Cheese Recipe (%) 원재료명Ingredients 배합비(%)Formulation ratio (%) 두유 발효물Fermented soy milk 82.7182.71 코코넛오일Coconut oil 10.0010.00 전분Starch 1.201.20 타피오카전분Tapioca starch 0.800.80 정제염Purified salt 0.650.65 정백당Jungbang 4.004.00 잔탄검Xanthan gum 0.020.02 LBGLBG 0.020.02 구연산Citric acid 0.200.20 치즈향Cheese flavor 0.400.40 합계Sum 100100

[[ 실험예Experimental Example 3: 상기  3: 실시예Example  And 비교예Comparative Example 치즈의 일반성분 분석결과] General compositional analysis of cheese]

본 실험에서는 상기 수득한 콩치즈와 종래 시중에서 판매되고 있는 비교예 (필라델피아 크림치즈, 동서식품수입)의 탄수화물, 단백질, 지방, 포화지방, 나트륨, 초고형분 함량을 분석하였다.In this experiment, the contents of carbohydrates, proteins, fats, saturated fats, sodium and super solids of the soybean cheeses obtained in the past and commercially available comparative examples (imported from Philadelphia cream cheese and East-West food) were analyzed.

수분은 상압건조 가열법으로 정량하였으며, 조단백과 조지방은 AOAC방법을 사용하였으며, 조단백 분석시 질소환산계수는 6.25를 사용하였다.Moisture was determined by atmospheric pressure dry heating method. AOAC method was used for crude protein and crude fat, and 6.25 was used for crude protein analysis.

나트륨은 AOAC 방법을 이용하여 시료를 105℃의 drying oven에서 건조 및 예비 탄화하였다. 또한, 600℃ 회화로에서 완전히 회화될때까지 가열하였고, 회화된 재는 1mol 질산에 녹여 원자흡광광도계(PE 3300 Flame type)을 사용하여 분석하였다. (참조: 표 5)Sodium was dried and pre-carbonized in a drying oven at 105 ° C using the AOAC method. Also, the mixture was heated at 600 ° C until it was completely decolorized, and the ash was dissolved in 1 mol of nitric acid and analyzed using an atomic absorption photometer (PE 3300 Flame type). (See Table 5)

실시예 및 비교예의 일반성분 분석결과 ( /100g 기준)General compositional analysis results of the examples and comparative examples (on the basis of 100 g) 구분division 실시예Example 비교예Comparative Example 탄수화물(g)Carbohydrate (g) 1616 1212 당류(g)Sugars (g) 55 3.53.5 단백질(g)Protein (g) 99 66 지방(g)Fat (g) 1515 2828 포화지방(g)Saturated fat (g) 1111 2323 나트륨(mg)Sodium (mg) 3030 3636 열량(kcal)Calories (kcal) 234234 321321 총고형분(%)Total Solids (%) 4242 4747

분석결과, 상기 표 5에서 보듯이, 비교예 대비 실시예의 단백질 함량이 약 50%, 탄수화물이 33% 높게 나타났으며, 지방은 46%, 열량은 27% 낮게 나타났다. 총 고형분 함량은 비교예 대비 약 10.6% 정도 낮았다.As a result of the analysis, as shown in Table 5, the protein content of the comparative example was about 50%, the carbohydrate content was 33%, the fat content was 46%, and the calorie content was 27% lower. The total solid content was about 10.6% lower than the comparative example.

실시예의 컨셉인 100% 식물성, 식물성 단백질 공급가능, 비교예 대비 낮은 지방 및 열량으로 인한 다이어트식품 활용 등 기능적 측면으로 볼 때, 적절한 영양성분 구성으로 판단하였다.In terms of functional aspects such as 100% vegetable, the availability of vegetable protein which is the concept of the embodiment, low fat compared to the comparative example, and utilization of diet food due to the heat quantity, it was judged that proper nutritional composition was constituted.

[[ 실험예Experimental Example 4: 상기  4: 실시예Example  And 비교예Comparative Example 치즈의 이소플라본 분석 결과] Isoflavone analysis of cheese]

본 실험에서는 상기 수득한 실시예 콩 치즈의 이소플라본 함량을 확인하고자 하였다. 이소플라본은 콩에 존재하는 천연생리활성물질로 HPLC(Agilent 1260 series, USA)법으로 분석하였고, 비교예는 종래 시중에서 판매되는 크림치즈 (필라델피아 크림치즈, 동서식품수입)과 비교하였다. (참조: 표 6)In this experiment, the content of isoflavone of the soybean cheeses obtained in the above-mentioned Example was checked. Isoflavone was analyzed by HPLC (Agilent 1260 series, USA) as a natural physiologically active substance present in soybean, and a comparative example was compared with commercially available cream cheese (imported from Philadelphia cream cheese and East-West food). (See Table 6)

실시예 및 비교예 이소플라본 분석결과 (mg/100g 기준)EXAMPLES AND COMPARATIVE EXAMPLES Isoflavone analysis results (based on mg / 100 g) 구 분division 실시예Example 비교예Comparative Example DaidzinDaidzin 7.73 ±0.137.73 ± 0.13 0.000.00 M-DaidzinM-Daidzin 0.000.00 0.000.00 A-DaidzinA-Daidzin 0.69 ±0.110.69 + 0.11 0.000.00 GlycitinGlycitin 0.000.00 0.000.00 M-GlycitinM-Glycitin 0.000.00 0.000.00 A-GlycitinA-glycitin 0.46 ±0.030.46 + 0.03 0.000.00 GenistinGenistin 5.38 ±0.07 5.38 + 0.07 0.000.00 M-GenistinM-Genistin 0.000.00 0.000.00 A-GenistinA-Genistin 0.000.00 0.000.00 DaidzeinDaidzein 0.000.00 0.000.00 GlyciteinGlycitein 0.000.00 0.000.00 GenisteinGenistein 0.000.00 0.000.00 합계(mg/100g)Total (mg / 100g) 14.2614.26 0.000.00

실험결과, 실시예의 총 이소플라본 함량은 14.26 mg/100g 이었으며, 두유액 제조시 마쇄비율, 대두배아 유무 등 공정조건 차이에 따라 실시예 총 함량이 변경될 수 있으며, 비교예와 달리 콩치즈에 천연생리활성물질인 이소플라본을 함유하는 것은 콩치즈만이 가질 수 있는 강점으로 판단하였다.As a result, the total isoflavone content of the examples was 14.26 mg / 100 g, and the total content of the examples could be changed according to the difference in the processing conditions such as the degree of grinding and the presence of the soybean embryo in the production of soy milk. Containing isoflavone, which is a physiologically active substance, was judged to be a merit of only bean curd.

[[ 실험예Experimental Example 5: 상기  5: 실시예Example  And 비교예Comparative Example 치즈의  Cheese 텍스쳐texture 분석결과] Analysis]

본 실험에서는 상기 수득한 콩치즈와 비교예 치즈(필라델피아 크림치즈, 동서식품수입)의 조직감(텍스쳐)을 분석하였다. Texture analyzer(TA-XT Express, Stable Micro System Ltd, UK)를 사용하였으며, 견고성(hardness), 부착성(adhesiveness), 응집성(cohesiveness), 탄력성(springiness), 점착성(gumminess), 씹힘성(chewiness) 및 복원성(resilience)을 분석하였다. 측정은 총 3회 반복하여 얻어진 데이터를 통계처리 하였다. (참조: 표 7 및 8)In this experiment, texture of the obtained soybean cheese and comparative cheese (imported from Philadelphia cream cheese and East-West food) was analyzed. (TA-XT Express, Stable Micro System Ltd, UK) was used to measure the hardness, adhesiveness, cohesiveness, springiness, gumminess, chewiness, Resilience was analyzed. The data obtained by repeating the measurement three times in total were statistically processed. (See Tables 7 and 8)

실시예 및 비교예의 견고성, 씹힘성, 점착성 분석결과The firmness, chewiness, and tackiness analysis results of Examples and Comparative Examples 구분division HardnessHardness ChewinessChewiness GumminessGumminess 실시예Example 34.5034.50 19.4419.44 21.6421.64 33.5033.50 18.1018.10 20.0820.08 32.8032.80 19.1419.14 21.1021.10 33.60 ±0.8533.60 ± 0.85 18.89 ±0.7018.89 ± 0.70 20.94 ±0.7920.94 + 0.79 비교예Comparative Example 237.0237.0 144.93144.93 145.81145.81 206.0206.0 120.39120.39 122.60122.60 231.3231.3 152.11152.11 152.11152.11 224.77 ±16.50224.77 ± 16.50 137.17 ±14.55137.17 ± 14.55 140.17 ±15.54140.17 ± 15.54

실시예 및 비교예의 부착성, 탄력성, 응집성, 복원성 분석결과The adhesion, elasticity, cohesiveness and stability properties of the examples and comparative examples 구분division AdhesivenessAdhesiveness SpringinessSpringiness CohesivenessCohesiveness ResilienceResilience 실시예Example -43.6-43.6 0.900.90 0.630.63 0.120.12 -45.5-45.5 0.900.90 0.600.60 0.100.10 -44.2-44.2 0.910.91 0.640.64 0.100.10 -44.43 ±0.97-44.43 ± 0.97 0.90 ±0.010.90 + - 0.01 0.62 ±0.020.62 + 0.02 0.11 ±0.010.11 + - 0.01 비교예Comparative Example -286.7-286.7 0.990.99 0.620.62 0.050.05 -253.2-253.2 0.980.98 0.600.60 0.050.05 -330.5-330.5 0.960.96 0.660.66 0.060.06 -290.13 ±38.76-290.13 + - 38.76 0.98 ±0.020.98 + 0.02 0.63 ±0.030.63 + 0.03 0.05 ±0.010.05 ± 0.01

실험결과, 비교예 대비 실시예의 경도는 유의적으로 낮게 확인되었다.(p=0.05) 씹힘성과 점착성, 부착성 역시 비교예 대비 유의적으로 낮게 나타났으며, 탄력성, 응집성, 복원성은 유사한 수준으로 확인하였다.As a result of the test, the hardness of the example was significantly lower than that of the comparative example (p = 0.05). The chewiness, stickiness and adhesiveness were also significantly lower than those of the comparative example, and the elasticity, cohesiveness, Respectively.

[[ 실험예Experimental Example 6: 상기  6: 실시예Example  And 비교예Comparative Example 치즈의 색도 분석결과] Analysis of color of cheese]

본 실험에서는 상기 수득한 콩치즈와 비교예 치즈(필라델피아 크림치즈, 동서식품수입)의 색도를 분석하였다.In this experiment, the chromaticity of the soybean cheese obtained above and the comparative cheese (Philadelphia cream cheese, East-West food imported) was analyzed.

분석결과, 하기 표 9와 같았다. 색도는 색도계(Color Flex EZ, Hunterlab, USA)를 이용하여 명도(Lightness, L), 녹색도(Greenness, a), 황색도(Yellowness, b)를 측정하여 측정값을 근거로 ΔE값을 산출하여 색상 차이를 확인하였다. The results are shown in Table 9 below. The color was measured by measuring the lightness (L), greenness (a), and yellowness (b) using a colorimeter (Color Flex EZ, Hunterlab, USA) The color difference was confirmed.

실시예 및 비교예의 색도 분석결과Chromaticity analysis results of Examples and Comparative Examples 구분division Lightness, LLightness, L Greenness, aGreenness, a Yellowness, bYellowness, b 실시예Example 87.3287.32 -0.16-0.16 18.8718.87 87.5287.52 -0.19-0.19 18.9218.92 87.9287.92 -0.17-0.17 18.8218.82 87.59 ±0.3187.59 ± 0.31 -0.17 ±0.02-0.17 0.02 18.87 ±0.0518.87 ± 0.05 비교예Comparative Example 90.1990.19 2.192.19 20.7620.76 90.2590.25 2.222.22 20.6920.69 90.4490.44 2.242.24 20.7220.72 90.29 ±0.1390.29 + 0.13 2.22 ±0.032.22 ± 0.03 20.73 ±0.0420.73 + 0.04 ΔEΔE 3.9 (현저한 차이)3.9 (Significant difference)

실험결과, 명도(L값)는 실시예 대비 비교예가 약간 높게 나타났으며, 실시예 대비 비교예가 보다 밝은 것으로 확인하였다. 치즈의 기호도에 영향을 미치는 녹색도(a값)는 실시예가 비교예 대비 낮게 나타났으며, 치즈 색감으로 인식되는 황색도(b값)는 비교예 대비 약간 낮은 것으로 확인하였다. As a result of the experiment, the brightness (L value) of the comparative example is slightly higher than that of the embodiment, and it is confirmed that the comparative example is brighter than the embodiment. The greenness (a value) which affects the preference of cheese was lower than that of the comparative example, and the yellowness (b value) recognized as cheese color was slightly lower than the comparative example.

[실험예 7: 상기 실시예 및 비교예 치즈의 관능분석 결과][Experimental Example 7: Sensory Evaluation Results of the Cheese and the Comparative Example Cheese]

본 실험에서는 상기 수득한 실시예와 비교예(필라델피아 크림치즈, 동서식품수입)의 관능적 특성을 비교하였다. 관능검사원 20-30대 45명과 50대 이상 일반 평가원 21명을 대상으로 색상, 고소함, 치즈풍미, 발림성 등 치즈의 전반적인 특성에 대해 5점 척도를 기준으로 평가하였다. 기호도가 높을수록 5점에 가까운 점수를 주어 선호도 및 만족도를 평가하였다.In this experiment, the sensory characteristics of the above-mentioned Examples and Comparative Examples (imported from Philadelphia cream cheese and East-West food) were compared. The overall characteristics of cheese such as color, flavor, cheese flavor, and spreadability were evaluated on a 5-point scale for 45 sensory laboratories and 20 ordinary evaluators over 50 generations. The higher the preference score, the closer the score was to 5, and the preference and satisfaction were evaluated.

(참조: 표 10 내지 15)(See Tables 10 to 15)

실시예 및 비교예의 세부묘사분석결과 (20-30대 패널 45명)Detailed analysis of the examples and comparative examples (20-30 panels, 45 persons) 구분division 강도burglar 만족도satisfaction 실시예Example 비교예Comparative Example 실시예Example 비교예Comparative Example 색상color 3.003.00 2.762.76 3.883.88 3.723.72 고소함Compliment 3.523.52 3.683.68 3.723.72 3.843.84 치즈풍미Cheese flavor 3.603.60 3.803.80 3.723.72 3.923.92 식감Texture 3.843.84 3.523.52 3.763.76 3.883.88 발림성Brittle 4.254.25 3.893.89 3.603.60 3.333.33

실시예 및 비교예의 외관 기호도 평가 (20-30대 패널 45명)Appearance preference evaluation of Examples and Comparative Examples (20-30 panels: 45 panelists) 구분division T.BT.B T.PT.P MM T.NT.N 실시예Example 7%7% 67%67% 3.73 ±0.583.73 ± 0.58 0%0% 비교예Comparative Example 13%13% 71%71% 3.84 ±0.643.84 0.64 0%0% T.B : top box로 매우좋다(5점척도 기준) 5점 응답률
T.P : top positive로 매우좋다(5점)+좋다(4점) 응답률
M : mean으로 평균의미
T.N : top negative로 싫다(2점)+매우싫다(1점) 응답률
TB: very good as top box (based on 5 point scale) 5 point response rate
TP: very good as top positive (5 points) + good (4 points) Response rate
M: Mean means mean
TN: I do not like top negative (2 points) + I hate it very much (1 point) Response rate

실시예 및 비교예의 관능 기호도 평가 (20-30대 패널 45명)Evaluation of sensory evaluation of the sensory evaluation of the examples and comparative examples (20-30 panels: 45 people) 구분division T.BT.B T.PT.P MM T.NT.N 실시예Example 2%2% 71%71% 3.73 ±0.503.73 + - 0.50 0%0% 비교예Comparative Example 4%4% 82%82% 3.87 ±0.463.87 ± 0.46 0%0% T.B : top box로 매우좋다(5점척도 기준) 5점 응답률
T.P : top positive로 매우좋다(5점)+좋다(4점) 응답률
M : mean으로 평균의미
T.N : top negative로 싫다(2점)+매우싫다(1점) 응답률
TB: very good as top box (based on 5 point scale) 5 point response rate
TP: very good as top positive (5 points) + good (4 points) Response rate
M: Mean means mean
TN: I do not like top negative (2 points) + I hate it very much (1 point) Response rate

실시예 및 비교예의 세부묘사분석결과 (50대 이상 패널 21명)Detailed analysis of the examples and comparative examples (21 panelists over 50) 구분division 강도burglar 만족도satisfaction 실시예Example 비교예Comparative Example 실시예Example 비교예Comparative Example 색상color 3.383.38 3.133.13 4.004.00 3.503.50 고소함Compliment 3.753.75 3.503.50 4.254.25 3.503.50 치즈풍미Cheese flavor 3.503.50 3.753.75 3.883.88 3.633.63 식감Texture 3.883.88 3.383.38 4.254.25 3.503.50 발림성Brittle 4.254.25 3.253.25 4.384.38 3.383.38

실시예 및 비교예의 외관 기호도 평가 (50대 이상 패널 21명)Evaluation of appearance preference of the examples and comparative examples (21 panelists of 50 or more) 구분division T.BT.B T.PT.P MM T.NT.N 실시예Example 25%25% 100%100% 4.25 ±0.464.25 + - 0.46 0%0% 비교예Comparative Example 0%0% 60%60% 3.75 ±0.463.75 + - 0.46 0%0% T.B : top box로 매우좋다(5점척도 기준) 5점 응답률
T.P : top positive로 매우좋다(5점)+좋다(4점) 응답률
M : mean으로 평균의미
T.N : top negative로 싫다(2점)+매우싫다(1점) 응답률
TB: very good as top box (based on 5 point scale) 5 point response rate
TP: very good as top positive (5 points) + good (4 points) Response rate
M: Mean means mean
TN: I do not like top negative (2 points) + I hate it very much (1 point) Response rate

실시예 및 비교예의 관능 기호도 평가 (50대 이상 패널 21명)Evaluation of sensory evaluation of the sensory evaluation of the examples and comparative examples (21 panelists of 50 or more) 구분division T.BT.B T.PT.P MM T.NT.N 실시예Example 37.5%37.5% 100%100% 4.38 ±0.524.38 ± 0.52 0%0% 비교예Comparative Example 0%0% 62.5%62.5% 3.63 ±0.523.63 ± 0.52 0%0% T.B : top box로 매우좋다(5점척도 기준) 5점 응답률
T.P : top positive로 매우좋다(5점)+좋다(4점) 응답률
M : mean으로 평균의미
T.N : top negative로 싫다(2점)+매우싫다(1점) 응답률
TB: very good as top box (based on 5 point scale) 5 point response rate
TP: very good as top positive (5 points) + good (4 points) Response rate
M: Mean means mean
TN: I do not like top negative (2 points) + I hate it very much (1 point) Response rate

실험결과, 20~30대 패널에서는 비교예 대비 실시예 색상이 약간 강한 것으로 나타났으며, 만족도는 비교예가 높게 나타났다. 치즈 풍미는 비교예가 강하고 만족도가 높게 나타났으며, 식감은 실시예가 강하며 만족도는 비교예가 높게 나타났다. 고소한 맛은 비교예가 강하고 만족도가 높게 나타났으며, 발림성은 실시예가 우수한 편으로 평가되었다.Experimental results showed that the color of the example was slightly stronger than that of the comparative example in the 20th - 30th panel, and the satisfaction level was higher in the comparative example. Cheese flavor was stronger in comparison and higher in satisfaction, and the texture was strong in the examples and the satisfaction was higher in the comparative example. The comparative sample showed a high flavor and a high degree of satisfaction, and the spreadability was evaluated as excellent in the examples.

종합적인 외관 및 관능에 대한 평가 결과는 비교예 대비 실시예가 약간 높게 평가되었다.Comprehensive appearance and sensory evaluation results were slightly higher than the comparative example.

50대 이상 패널 평가 결과, 색상은 실시예가 비교예 대비 강하고 만족도도 높게 나타났다. 치즈 풍미는 비교예가 약간 강하다고 나타났으며 만족도는 실시예가 높게 평가되었다. 식감은 실시예가 유의적으로 높은 만족도를 나타났으며, 고소함 역시 실시예가 높게 평가되었다. 발림성은 실시예가 비교예 대비 유의적으로 우수하게 평가되었음을 확인하였다.As a result of the panel evaluation of 50 or more panels, the color of the embodiment was stronger than that of the comparative example, and the satisfaction was also high. The cheese flavor was slightly stronger in the comparative example and the degree of satisfaction was high in the examples. The texture was significantly higher in the examples, and the smell was also highly evaluated in the examples. The rinseability was evaluated to be significantly better than the comparative example.

종합적인 외관 및 관능에 대한 평가 결과, 실시예가 비교예 대비 유의적으로 높게 평가되었다.Comprehensive appearance and sensory evaluation showed that the examples were significantly higher than the comparative examples.

Claims (6)

두유에 유산균을 첨가하여 발효시켜 두유 발효물을 수득하는 단계 (a);
상기 단계 (a)에서 수득한 두유 발효물로부터 유청을 분리하여 제거하는 단계 (b);
상기 단계 (b)에서 유청이 제거된 두유 발효물에, 코코넛오일, 전분, 정제염, 정백당, 잔탄검, LBG(Locust Bean Gum)을 첨가하는 단계 (c); 를 포함하는 것을 특징으로 하는 콩치즈의 제조방법.
(A) adding soybean milk to lactic acid bacteria and fermenting the soybean milk to obtain a soybean fermented product;
(B) separating and removing whey from the soybean milk fermentation product obtained in the step (a);
(C) adding coconut oil, starch, purified water, white sugar, xanthan gum and LBG (locust bean gum) to the fermented soybean milk from which the whey has been removed in the step (b); &Lt; / RTI &gt;
제1항에 있어서,
상기 단계 (a)의 두유는,
대두에, 대두 중량의 2~6배의 열수를 첨가하여 열수 마쇄한 후, 여과하여 비지를 분리함으로써 수득된 것임을 특징으로 하는 콩치즈의 제조방법.
The method according to claim 1,
The soy milk of step (a)
Wherein the soybean is obtained by adding hot water of 2 to 6 times the weight of soybeans to the soybeans and subjecting the soybeans to hot water grinding and filtration to separate the beans.
제2항에 있어서,
상기 대두는,
비린내를 제거하기 위하여 열처리된 것을 특징으로 하는 콩치즈의 제조방법.
3. The method of claim 2,
The soybean,
Characterized in that it is heat-treated to remove fishy flesh.
제1항에 있어서,
상기 단계 (a)의 유산균 발효시,
프락토올리고당(fructooligosaccharide)을 첨가하는 것을 특징으로 하는 콩치즈의 제조방법.
The method according to claim 1,
In the step (a) of fermenting lactic acid bacteria,
Wherein the fructooligosaccharide is added to the bean curd.
제1항에 있어서,
상기 단계 (a)의 유산균은,
락토바실러스 불가리쿠스 (Lactobacillus bulgaricus)와 스트렙토코커스 써모필러스(Streptococcus thermophilus)를 포함하는 복합 유산균 제제인 것을 특징으로 하는 콩치즈의 제조방법.
The method according to claim 1,
The lactic acid bacteria of step (a)
A method for producing soybean cheese characterized by being a complex lactic acid bacterial preparation comprising Lactobacillus bulgaricus and Streptococcus thermophilus .
제1항에 있어서,
상기 단계 (b)의 유청 분리 및 제거는,
두유 발효물을 면포에 담아 유청을 분리하여 제거시키는 것을 특징으로 하는 콩치즈의 제조방법.

The method according to claim 1,
The whey separation and removal of the step (b)
Wherein the soybean milk fermented product is contained in cottonseed to separate and remove the whey.

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210056017A (en) 2019-11-08 2021-05-18 주영철 High yield manufacturing method of vegetable fermented cheese
KR20210058347A (en) 2019-11-14 2021-05-24 주영철 High yield manufacturing method of vegetable fermented cheese
KR20220160332A (en) 2021-05-27 2022-12-06 박현진 Vegan cheese composition and manufacturing method thereof
WO2022261450A1 (en) * 2021-06-11 2022-12-15 Kraft Foods Group Brands Llc Plant-based cream cheese product and method of making a plant-based cream cheese product
EP4122328A4 (en) * 2020-03-16 2024-04-03 Kikkoman Corp Fermented soybean beverage concentrate and production method thereof

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KR101311994B1 (en) 2012-03-12 2013-09-27 유승익 Producing method of cheese
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KR101311994B1 (en) 2012-03-12 2013-09-27 유승익 Producing method of cheese
KR101466366B1 (en) 2013-01-10 2014-11-28 재단법인 임실치즈과학연구소 Manufacturing method for romano cheese

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210056017A (en) 2019-11-08 2021-05-18 주영철 High yield manufacturing method of vegetable fermented cheese
KR20210058347A (en) 2019-11-14 2021-05-24 주영철 High yield manufacturing method of vegetable fermented cheese
EP4122328A4 (en) * 2020-03-16 2024-04-03 Kikkoman Corp Fermented soybean beverage concentrate and production method thereof
KR20220160332A (en) 2021-05-27 2022-12-06 박현진 Vegan cheese composition and manufacturing method thereof
WO2022261450A1 (en) * 2021-06-11 2022-12-15 Kraft Foods Group Brands Llc Plant-based cream cheese product and method of making a plant-based cream cheese product

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