KR20190071381A - enzyme-linked immunosorbent assay apparatus having LED lighting - Google Patents

Abstract

Disclosed are a device and a method for blood-based in vitro diagnosis using an LED which can increase accuracy of diagnosis by emitting an LED light source to a reactant in multiple channels and sensing the LED light source. According to the present invention, the device for blood-based in vitro diagnosis using an LED comprises: a well plate having a plurality of seating grooves on which blood and a reagent are seated while the blood and the reagent react with each other; a plurality of LED light sources which are arranged on a PCB in a line to emit light to the well plate in a single wavelength only; a plurality of optical sensors to detect a quantity of light emitted to the well plate to penetrate the well plate; an analog digital converter to convert analog data collected by the plurality of optical sensors into digital data; and a control unit to receive the digital data received from the digital converter, and derive a diagnosis result in accordance with a previously stored program. The control unit sequentially turns on and off the plurality of LED light sources to prevent interference by adjacent LED light sources.

Description

[0001] The present invention relates to an enzyme-linked immunosorbent assay apparatus having LED illumination,

The present invention relates to a blood-based ex vivo diagnostic apparatus and method using an LED capable of enhancing the accuracy of diagnosis by irradiating an LED light source to a reactant in multiple channels and detecting the same.

In general, the in vitro diagnostic industry is in the spotlight in the recent era of preventive and personalized medicine. The in vitro diagnostic industry is a field that can be identified by blobs and urine to avoid various diseases such as diabetes, cholesterol, cancer, etc. It has already been introduced to medical institutions such as hospitals and used for many diagnoses. In addition, in vitro diagnostic devices capable of identifying various diseases have been developed.

Core competencies in the development of in vitro diagnostic devices are the convergence of biotechnology that develops biomaterials (antigens, antibodies, genes, enzymes, etc.) that react with target substances and information technology that develops instruments to measure them. Most in-vitro diagnostics companies in the world are leading the high-value-added knowledge-based industries by creating huge sales and net profits through such technology convergence.

The in vitro diagnostic system reacts the target substance and the antigen in the reactor, irradiates the light having the predetermined spectrum in the light emitting part, measures the light reflected by the light receiving part, the transmitted light and the incident light passing therethrough and compares the measured light with the predetermined examples Diagnosis is made.

Since the conventional external diagnosing apparatus normally emits light using a lamp as a light emitting portion, a device such as a lens and a filter optical fiber must be inserted between the reactor and the lamp for providing a visible light in order to irradiate light of the correct wavelength depending on the object, There has been a problem of becoming complicated.

Referring to FIG. 1, a light emitting portion and a light receiving portion of an in vitro diagnostic apparatus according to the prior art are shown. A plurality of lamps 1 are provided on the PCB substrate, and the lamps 1 are turned on and off simultaneously by the control unit 10. [ A lens 2 and a filter 3 for guiding and filtering the light emitted from the lamp 1 are provided on the lamps 1 and a well plate 4) are installed. In the well plate 4, a solution in which a target substance, an antigen, and an antibody are reacted is contained in a liquid state.

An optical sensor 5 is provided on the well plate 4 to sense and sense the light transmitted through the reactant of the well plate 4 and analogue digital converters 6 are connected to the optical sensors 5, Each converter 6 is connected to the controller 10, digitizes the sensed contents, and transmits the digitized contents to the controller 10.

The control unit 10 controls the apparatus by turning on and off the lamp 1 by controlling it through a circuit and a program, and controls the apparatus as a whole, and transmits the calculated result to the outside through communication, display, or the like.

However, since the conventional in-vitro diagnosing apparatus is structured such that the lamps are turned on and off at the same time, diagnosis may be inaccurate due to optical interference between the lamps, and lighting and light-off are slow, And an optical fiber is used to prevent heat transfer. Therefore, since an analog digital converter is required to be connected to each optical sensor, the number of components is increased, resulting in an increase in cost and complication of the device.

US 8,940,249 B2

It is an object of the present invention to provide a blood-based ex vivo diagnostic apparatus and method using an LED capable of rapidly diagnosing a plurality of reactants using a plurality of LED light sources.

As a specific means for achieving the above object, the present invention provides a well plate comprising a plurality of seating grooves seated in a state in which a reaction of blood and a reagent is performed; A plurality of LED light sources arranged in a line on the PCB concentrically to the well plate so as to irradiate light to the well plate and emitting light only at one wavelength; a plurality of photosensors for detecting the amount of light irradiated to the well plate; An analog digital converter for converting the analog data collected by the plurality of optical sensors into digital data; And a control unit for receiving the digital data received from the digital converter and deriving a diagnosis result according to a program stored in advance, wherein the control unit sequentially turns on and off the plurality of LED light sources so as not to be interfered by the adjacent LED light sources, Off control can be performed.

Advantageously, said plurality of optical sensors can be connected to one analog digital converter.

Advantageously, the plurality of LED light sources and the plurality of photosensors are fixed to a frame, and the well plate can be seated on a transport tray that linearly moves between the plurality of LED light sources and the plurality of photosensors.

According to another aspect of the present invention for achieving the above object, the present invention provides a method of manufacturing a blood pressure monitor, comprising: preparing a well plate in a state where blood is provided in each of a plurality of seating grooves; Providing the well plate with a reagent and reacting; Sequentially turning on and off the plurality of LED light sources so that a light source is provided to each of the reactants reacted to the well plate; The optical sensor detects an image transmitted through the reactant, the information is converted into digital information by an analog digital converter, and then the digital information is transmitted to a controller; And receiving and analyzing the digital information in the control unit and displaying the result of the analysis, wherein information detected in each of the optical sensors can be sequentially converted in one analog digital converter.

The present invention as described above has the following effects.

(1) Since an LED is used as a light source, a device such as a lens and a filter is not required, and the device can be downsized.

(2) Since the present invention can use only one analog digital converter by controlling the LED as a light source in multiple channels, it is possible to reduce the number of components and avoid the interference of the light source, thereby improving diagnostic reliability.

FIG. 1 is a block diagram of a diagnostic unit including a light emitting unit and a light receiving unit, which are components of a conventional blood-based ex vivo diagnostic apparatus.
FIG. 2 is a block diagram of a diagnostic unit, which is a component of a blood-based ex vivo diagnostic apparatus using an LED according to the present invention.
3 is an overall perspective view showing an internal configuration of a blood-based ex vivo diagnostic apparatus using an LED according to the present invention.
4 is a perspective view of a diagnosis unit which is a component of a blood-based ex vivo diagnostic apparatus using an LED according to the present invention.
FIG. 5 is a plan view of a diagnosis unit, which is a component of a blood-based ex vivo diagnostic apparatus using an LED according to the present invention.
6 is a side view of a diagnosis unit which is a component of a blood-based ex vivo diagnostic apparatus using LED according to the present invention.
7 is a flowchart of a blood-based in vitro diagnostic method using an LED according to the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, which will be readily apparent to those skilled in the art to which the present invention pertains. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and the same or similar components are denoted by the same reference numerals throughout the specification.

In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, components, parts, or combinations thereof. Also, where a section such as a layer, a film, an area, a plate, or the like is referred to as being "on" another section, it includes not only the case where it is "directly on" another part but also the case where there is another part in between. On the contrary, where a section such as a layer, a film, an area, a plate, etc. is referred to as being "under" another section, this includes not only the case where the section is "directly underneath"

Hereinafter, an apparatus and method for blood-based ex vivo diagnosis using an LED according to an embodiment of the present invention will be described in detail with reference to the drawings.

2 to 6, the IVD 100 according to the first embodiment of the present invention includes a well plate 143, a plurality of LED light sources 142, a plurality of optical sensors 144 An analog-to-digital converter 145, and a control unit 146.

The well plate 143 has a plurality of seating grooves that are seated in a state in which blood and a reagent are reacted.

At this time, when the reagent is supplied to the plurality of seating grooves, the well plate 143 becomes a reactant which can be diagnosed by being diluted. Referring to FIG. 3, the well plate 143 is seated on the conveyance tray 147, and is conveyed together as the conveyance tray 147 is linearly driven.

The plurality of LED light sources 142 and the plurality of photosensors 144 are fixed to the frame 101 of the entire equipment and the well plate 143 includes the plurality of LED light sources 142, And is mounted on a conveyance tray 147 that linearly moves between the optical sensors 144.

At this time, when the reagent is supplied to the well plate 143, the transfer plate 147 transfers the well plate 143 to the incubator 160. The well plate 143 enters the incubator 160 and is reacted.

At this time, the well plate 143 reacted in the incubator 160 is diagnosed as the transfer plate 147 is released. This is because the plurality of LED light sources 142 and the optical sensor 144 are provided on the entrance / exit side of the incubator 160.

Referring to FIGS. 2 and 6, the plurality of LED light sources 142 are arranged in a line on the PCB 141 to emit light to the well plate 143, and emit light at only one wavelength.

At this time, the wavelength of the plurality of LED light sources 142 is 450 nm.

At this time, since the plurality of LED light sources 142 emit light with only one wavelength, a filter or the like is unnecessary, and the module of the LED light source 142 uses straight light instead of diffused light, so that a lens or the like is unnecessary . Therefore, as shown in FIG. 6, the well plate 143 is installed in close contact with the LED light source 142.

Referring to FIGS. 2 and 6, the plurality of photosensors 144 detect light transmitted through the well plate 143.

At this time, the plurality of optical sensors 144 are installed on the LED light sources 142 at positions corresponding to the respective LED light sources 142. Accordingly, the optical sensor 144 is installed at a position where each LED light source 142 is transmitted, and the transmitted light is detected.

The analog-to-digital converter 145 converts the analog data collected by the plurality of optical sensors 144 into digital data.

At this time, the plurality of optical sensors 144 are connected to one analog digital converter 145, referring to FIG.

This is because the plurality of LED light sources 142 are not simultaneously turned on and off but are sequentially collected by the plurality of optical sensors 144 because the light is turned on and off with a time difference in multiple channels to be. Of course, the time difference between turning on and off the plurality of LED light sources 142 is so short that it is difficult for a person to recognize, so that the diagnosis does not take a long time.

Referring to FIG. 2, the controller 146 receives the digital data received from the analog-to-digital converter 145 and derives a diagnostic result according to a pre-stored program.

The controller 146 is connected to the PCB 141 and sequentially turns on and off the plurality of LED light sources 142 so that the LED light sources 142 do not interfere with each other.

In addition, the controller 146 controls the operation of other devices.

Referring to the block diagram of FIG. 2, the controller 146 is shown at the top. The controller 146 is connected to the PCB 141 to sequentially turn on and off the plurality of LED light sources 142. Here, the number of the LED light sources 142 is eight. The well plate 143 is located above the plurality of LED light sources 142 and the plurality of photosensors 144 are also located thereon. The plurality of optical sensors 144 are connected to one analog digital inverter 145 and the analog digital inverter 145 is connected to the controller 146 to provide the information.

Referring to FIG. 3, the entire configuration of the in vitro diagnostic apparatus 1 is shown, in which various components are installed inside the frame 101, and the front cover is removed. The in vitro diagnostic apparatus 100 includes a diagnosis unit 140 for performing measurement and diagnosis, a reagent supply unit 120 for supplying a reagent to the diagnosis unit 140, And a waste liquid treatment unit 180 for treating a waste liquid such as a liquid for washing and washing. The present invention relates to the configuration of the diagnosis unit (140).

The in vitro diagnostic apparatus 100 supplies the reagent to the well plate 143 from the reagent supply unit 120 when the well plate 143 in a state in which blood is supplied is supplied to the transfer tray 147. The reagent supply unit 120 inserts the tip of the pipette device into the tip of the pipette while lifting the tip of the pipette device to the tip supply unit 130. The tip will be removed by the post-use removal device 170 because it is disposable. Then, the reagent supply unit 120 sucks the reagent from the reagent bottle 110 and then moves to the diagnosis unit 140 to discharge the reagent to the well plate 143 of the diagnosis unit 140. A total of eight pipettes will work here.

Thereafter, the LED light source 142, the light sensor 144, the control unit 146, and the like are diagnosed and the diagnosis result is provided.

After the diagnosis result is obtained, the reactant in the mounting groove of the well plate 143 is sucked and discharged by the suction nozzle of the cleaning equipment 150, and then the cleaning is performed by the cleaning nozzle of the cleaning equipment 150. At this time, the waste liquid generated by the suction nozzle and the cleaning nozzle collects in the waste liquid processing unit 180.

Referring to FIGS. 4 and 5, the well plate 143 is positioned below the incubator 160. The incubator 160 is fixed to the entire apparatus frame 101 and the well plate 143 is seated on the transfer plate 147. Therefore, the diagnosis is made while the well plate 143 moves linearly. Here, eight seating grooves are formed on one row of the well plate 143.

6, optical sensors 144 are fixedly mounted on the lower surface of the incubator 160 and the LED light sources 142 are installed on the upper surface of the PCB 141 at positions corresponding to lower portions of the optical sensors 144, And the well plate 143 is passed between the two plates. As a result, the well plate 143 having a plurality of rows formed with the mounting grooves on one row is transported by the transport plate 147 in a line or two lines (when the LED light source and the optical sensor are both provided in two lines) The measurement is made and a large number of diagnoses can be performed at one time.

7, a blood-based in vitro diagnostic method using an in vitro diagnostic apparatus using LEDs comprises a step (S1) of providing a well plate in a state where blood is provided in each of a plurality of seating grooves, a step (S4) of sequentially lighting and extinguishing a plurality of LED light sources so that a light source is provided to each of the reactants reacted to the well plate; and a step (S4) of irradiating the reactant (S5) of detecting light emitted from the optical sensor, converting the information into digital information by the analog digital converter and then transmitting the digital information to the control unit, receiving and analyzing the digital information in the control unit, and displaying the result S6), and the information detected by each of the optical sensors is sequentially converted by one analog digital converter.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

100: In Vitro Diagnostic Device
141: PCB (control board 142: LED light source
143: well plate 144: light sensor
145: analog digital inverter 146:

Claims (4)

A well plate having a plurality of seating grooves seated in a state in which a reaction of blood and a reagent is performed;
A plurality of LED light sources arranged in a line on the PCB concentrically with the well plate to emit light to the well plate and emitting light at only one wavelength,
A plurality of photosensors for detecting light transmitted through the well plate;
An analog digital converter for converting the analog data collected by the plurality of optical sensors into digital data;
A controller receiving the digital data received from the digital converter and deriving a diagnosis result according to a program stored in advance;
, ≪ / RTI &
Wherein the control unit sequentially turns on and off the plurality of LED light sources so as not to be interfered by adjacent LED light sources. The method according to claim 1,
Wherein the plurality of optical sensors are connected to one analog digital converter. The method according to claim 1,
Wherein the plurality of LED light sources and the plurality of photosensors are fixed to a frame, and the well plate includes a plurality of LED light sources and a blood-based extracorporeal diagnostic apparatus using an LED mounted on a transfer tray that linearly moves between the plurality of photosensors . Providing a well plate in a state where blood is provided in each of a plurality of seating grooves;
Providing the well plate with a reagent and reacting;
Sequentially turning on and off the plurality of LED light sources so that a light source is provided to each of the reactants reacted to the well plate;
Detecting light transmitted through the reactant by the optical sensor, converting the information into digital information by an analog digital converter, and then transmitting the digital information to a controller;
Receiving and analyzing digital information in the control unit and displaying the result;
, ≪ / RTI &
Wherein the information detected by each of the optical sensors is sequentially converted by one analog digital converter.

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