KR20190063080A - Red ginseng extranct improving a decreased immunomodulating ability and method for preparing the same - Google Patents

Abstract

The present invention relates to a red ginseng extract improving the immunomodulatory ability lowered by high temperature stress and a production method thereof and, more specifically, to a red ginseng extract which does not only merely improve immunity, but improves the immunomodulatory ability lowered by high temperature stress, and thus, can prevent or treat autoimmune diseases such as hyperthyroidism, acquired hemolytic anemia, chronic joint rheumatism, etc., and inflammatory reaction, and a production method thereof.

Description

[0001] The present invention relates to an extract of red ginseng which improves the immunomodulating ability deteriorated by high temperature stress and a method for preparing the same,

The present invention relates to a red ginseng extract which improves immunoregulatory ability degraded by high temperature stress and a method for producing the same. In particular, the present invention relates to a method for treating a patient suffering from thyroid hyperinsulinism, acquisitive hemolytic anemia, chronic joint rheumatism, or the like, which may be caused by a decrease in immunomodulating ability by improving the immunomodulating ability degraded by high- Which is capable of preventing or treating immune diseases or inflammatory reactions, and a method for producing the same.

The first cause of impaired immune regulation is autonomic imbalance, or stress. When stressed, the sympathetic nerves become tense, and the immune regulating ability is lowered, resulting in a decrease in immunity or an autoimmune disease or an inflammatory reaction due to an excessively immune state. The mind affects the nervous system, and the nervous system again affects the immune system. In fact, all autoimmune disease patients say they have had extreme stress.

This decrease in immunoregulatory ability is not merely a way to improve immunity, but it is difficult to heal, and the cause of the decrease in immunoregulatory ability is found. Natural remedy, which is a cause of correcting it, takes a long time and causes various complications before healing have.

On the other hand, Korean ginseng (Panax ginseng C. A. Meyer) has been used as long-term herbal medicine or long-lived health food. Experimentally, Korean ginseng has been evaluated to have little toxicity and no significant side effects have been found in many clinical trials until now.

Korean ginseng is classified as Yang (Yang) and Korean ginseng as Yin (Yin), so Korean ginseng has heat, and American ginseng has the effect of lowering heat. .

In particular, red ginseng is an oncological medicine that promotes blood circulation by warming the body and promoting metabolism of the body. Through these actions, various effects such as anti-hypertension, anti-diabetic, anti-obesity, Radiation protection, and improvement of learning ability. However, it is recognized that it is better to avoid people who have a lot of body heat due to heat in one room, for example, when they have a cold or a high temperature.

In addition, the red ginseng concentrate may have different kinds and contents of saponin contained in the concentrate depending on the extraction mode and conditions, and thus may have greatly different efficacies. Especially, There have been no reports on the kinds and contents of saponins having the preventive or therapeutic effect of autoimmune diseases such as thyroid hyperinsulinism, acquired hemolytic anemia, chronic joint rheumatism, or inflammatory reaction induced by the immunomodulatory ability.

Therefore, the present invention is not limited to simply improving the immune system, but may be applied to an autoimmune disease such as thyroid hyperinsulinism, acquisitive hemolytic anemia, chronic joint rheumatism, or the like, which may be caused by a decrease in immunoregulatory ability, A red ginseng extract that can prevent or treat the reaction and a method for producing the same are strongly desired.

It is an object of the present invention to provide a red ginseng extract which can improve not only the immunity but also the immunoregulatory ability deteriorated by the high temperature stress.

It is another object of the present invention to provide a method for preparing red ginseng extract by the kind and content of a specific saponin which realizes an effect of improving immunoregulatory ability deteriorated by high-temperature stress.

In order to solve the above problems,

(R), Rg3 (S), Rg2 (S), and Re, and has an effect of improving the immunomodulating ability deteriorated by high temperature stress, wherein Rb1 Wherein the content of Rg3 (S) is 1.74 to 2.165% by weight, the content of Rg3 (S) is 1.162 to 1.246% by weight, the content of Rg2 (S) is 0.497 to 0.565% by weight and the content of Re is 0.109 to 0.177% , And red ginseng extract.

Wherein the ginsenoside Rg1 is further contained, and the content of Rg1 is 0.16% by weight or less.

Also, the content of ginsenoside Rg1 is 0.05 to 0.16% by weight.

Further, the content of Rb1 is 1.80 to 2.166% by weight, the content of Rg3 (S) is 1.00 to 1.246% by weight, the content of Rg2 (S) is 0.50 to 0.565% by weight, and the content of Re Is 0.109 to 0.177% by weight, based on the total weight of the red ginseng extract.

The red ginseng extract of the present invention is characterized in that the total weight of ginsenosides is 3.554 to 5.513% by weight based on the total weight of the red ginseng extract.

And a health food containing the red ginseng extract.

On the other hand, there is provided a process for producing red ginseng comprising the steps of extracting red ginseng from water or ethyl alcohol, concentrating the supernatant of the precipitate after precipitation, sterilizing the concentrate, and aging the concentrate at 70 to 110 ° C for 40 to 56 hours. A method for preparing red ginseng extract is provided.

Herein, the step of aging the concentrated liquid is performed at 90 to 110 ° C. for 48 to 56 hours.

Also, the present invention provides a method for preparing red ginseng extract, wherein the red ginseng is extracted with purified water corresponding to 8 times or more the weight of the raw ginseng when extracting the red ginseng with water.

The precipitation is performed at 8 to 10 DEG C for at least 12 hours, and the concentration is performed by first concentrating the supernatant at 30 or more Brix and secondarily concentrating at 70 or more Brix , And a method for producing red ginseng extract.

The red ginseng extract according to the present invention can improve the immunoregulatory ability deteriorated by the high temperature stress through the combination of the kinds and the contents of the specific saponins, and thus the thyroid immunosuppression, acquired hemolytic anemia, Rheumatism and other autoimmune diseases or inflammatory reactions.

In addition, the method for preparing red ginseng extract according to the present invention is characterized in that the red ginseng extract produced by the precisely controlled and controlled process conditions has the kind and content of the specific saponin, so that the effect of improving the immunoregulatory ability deteriorated by the high temperature stress And exhibits excellent effects to be implemented.

FIG. 1 schematically shows a method for preparing red ginseng extract according to the present invention.
FIG. 2 is a graph showing the results of analyzing the components of the red ginseng extract prepared in the examples by HPLC chromatography analysis.
FIG. 3 is a graph showing the results of the daily mean insulin dose analysis for 6 weeks per experimental animal.
4 is a graph showing the results of serum analysis among blood samples in the examples.
5 is a graph showing plasma analysis results among blood samples in the examples.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, the embodiments disclosed herein are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

The extract of red ginseng according to the present invention is characterized in that it contains carbohydrates such as starch as an extract of red ginseng, ginsenoside which is ginseng saponins of triterpenoid system as a physiologically active substance, polyacetylene having anticancer activity, A phenolic compound having action, a ginseng protein having a radiation-protecting action, an acidic polysaccharide having an immunoregulatory action, and the like.

The chemical structure of the ginsenoside was confirmed by the Shibata group of the University of Tokyo. The ginseng saponin contained in the Korean ginseng is about 30 kinds, including 14 kinds of western ginseng and 15 kinds of Panax notoginseng Which contains much more variety of ginseng saponin.

The ginsenoside is a glycoside composed of glycone sugar and a non-sugar aglycone or genin, and in particular, a non-sugar portion of ginsenoside It is called "sapogenin." Ginsenosides are classified into two types according to the skeletal structure of the nonsugar part, namely, tetrameric dammarane system and 5-oleoan oleane system. Dendritic system ginsenoside Again, the protopanaxadiol ginsenoside is divided into 2 groups according to the number of hydroxyl groups (-OH) attached to the nonspecific part, and protopanaxadiol ginsenoside in the case of 3 groups.

Specifically, the pharmacological actions of the ginsenosides are shown in the following manner. The oleanolic ginsenoside Ro (Ro) has antiinflammatory, detoxifying, antitrobin, platelet aggregation inhibiting, anti-hepatitis and macrophage activation do.

Rb1, Rb2, Rc, Rd, Rh2, Rg3 (S), Rg5, Rk1 and the like may be included as the prophronaxan diol type ginsenoside. Among them, Rb1 may include a central nervous system inhibitory action, (Insulin-like) action, cholesterol biosynthesis promoting action, plasmin activating action, RNA synthesis promoting action, hormone secretion promoting action, etc., and Rb2 Rc plays a central nervous system inhibitory action, inhibits RNA synthesis, stimulates the synthesis of serum protein, and promotes the secretion of adrenocorticotropic hormone. , Rhg 2 inhibits the proliferation of cancer cells, inhibits tumor growth, and Rg 3 (S) acts as a proinflammatory cytokine. Inhibition of cancer cell metastasis, vasodilation, protection of brain cell damage, inhibition of anticancer drug resistance, inhibition of platelet aggregation, and the like, and Rg5 inhibits cancer cell proliferation.

Re, Rf, Rg1, Rg2 (S) and the like can be included as the prophronomic triol ginsenosides. Re among them can be used for inhibiting the central nervous system, promoting DNA / RNA synthesis, activating plasmin, Stimulating hormone secretion, etc. Rf plays a role in analgesia related to neuronal cells. Rg1 plays a central nerve stimulating action, anti-fatigue action, fatigue action, memory / learning function improving action, DNA · RNA synthesis promoting action, Rg2 (S) improves memory decline due to vascular brain damage, improves memory, improves neuroprotection, and promotes vascular endothelial cell proliferation.

The present inventors have found that when the content of Rb1, Rg1, Rg3 (S), Rg2 (S), Re, etc. among the ginsenosides contained in the red ginseng extract is controlled to a specific content, The present inventors have completed the present invention.

In other words, when a person is exposed to high-temperature stress, the corticotropin releasing hormone (CRH) secretes from the hypothalamus, thereby releasing the adrenocorticotropic hormone (ACTH) from the pituitary gland and causing cortisol cortisol) is secreted into the blood, thereby affecting the immune regulating ability. The extract of the red ginseng according to the present invention suppresses the secretion of the above-mentioned corticotropin-stimulating hormone (ACTH) and / or cortisol, It is predicted that if the immunoregulatory capacity is lowered by improving the control ability, autoimmune diseases such as thyroid hyperinsulinism, acquired hemolytic anemia and chronic joint rheumatism or inflammatory reaction can be prevented or treated.

Specifically, the red ginseng extract according to the present invention is characterized in that the content of ginsenoside Rb1 is 1.74 to 2.166% by weight, preferably 1.80 to 2.166% by weight, the content of Rg3 (S) is 1.162 to 1.246% by weight, , Preferably 1.00 to 1.246 wt.%, Rg2 (S) 0.497 to 0.565 wt.%, Preferably 0.50 to 0.565 wt.%, And Re content 0.109 to 0.177 wt. The content of Rg1 may be 0.16% by weight or less, for example, 0.05 to 0.16% by weight.

The red ginseng extract according to the present invention may have a total ginsenoside content of about 3.554 to 5.513% by weight, preferably 3.554 to 4.782% by weight based on the total weight of the extract.

As described above, the red ginseng extract according to the present invention maintains the original efficacy of red ginseng by precisely controlled contents of the ginsenosides contained in the red ginseng extract, and at the same time, Can exhibit an excellent effect with an effect of improving ability.

FIG. 1 schematically shows a method for preparing red ginseng extract according to the present invention. As shown in Fig. 1, the red ginseng extract according to the present invention can be produced by a manufacturing method in which the following steps a) to d) are carried out in a sequential manner.

a) extracting red ginseng from water or ethyl alcohol

b) concentrating the supernatant of the precipitation tank after precipitation

c) sterilizing the concentrate

d) aging the concentrate for 40 to 56 hours at 70 to 110 캜

In step a), the red ginseng to be extracted may preferably be red ginseng for 4 years or more, and the red ginseng may remove foreign substances that can be visually recognized before extraction and remove iron powder with a magnetic bar having a magnetic force of 10,000 gauss or more .

When the red ginseng is extracted with water, extraction with purified water corresponding to 8 times or more the weight of the raw red ginseng and extraction at 80 ± 2 ° C for 24 hours can be repeated three times.

After extracting in step a) and before concentrating in step b), the extract may be filtered with 0.5 microfilter and magnetic residual iron powder with a magnetic force of 10,000 gauss or more may be further removed.

The step b) may be carried out, for example, in a first concentration step in which the extract solution is precipitated at 8 to 10 DEG C for 12 hours, and then the supernatant liquid in the precipitation tank is concentrated at 30 mm Brix or more at 700 mmHg and 60 DEG C, The concentrated concentrate may again be subjected to a second concentration step to concentrate again above 70 Brix. Meanwhile, the sterilization process in the step c) may be performed at, for example, 90 DEG C for 2 hours.

Meanwhile, in the step d), the concentrate may be aged at 90 to 110 ° C for 48 to 56 hours.

The red ginseng extract according to the present invention can be produced in a solid phase such as a liquid phase, a powder, a pill or a capsule, and it can be produced, distributed and consumed as a beverage, a functional food, a health food or the like such as a tea, an extract, a drink, .

[Example]

1. Manufacturing Example

According to the preparation method according to the present invention, red ginseng extract (having a viscosity of about 150,000 cPs, a solid content of about 61.56%, a dark brown color, a brightness of 22,47 ± 2.82, a redness of 2.26 ± 1.08, and a yellowness of 15.87 ± 1.59) was prepared, and component analysis was performed by HPLC chromatography analysis. The results are shown in FIG. 2 and Table 1 below.

Gin Senocide Content (% by weight) Rb1 1.953 Rg3 (S) 1.204 Rg2 (S) 0.531 Re 0.143 Rg1 0.032

2. Evaluation of efficacy to improve high temperature stress and thereby diminished immunomodulating ability

(1) Observation of behavior and evaluation of the amount of water

Male rats (SD), 7 weeks old, as an experimental animal were purchased from Orient Bio Co., Ltd., and after a week of adaptation period, a high temperature environment unit of 260 mm × 800 mm × 1,830 mm size (volume: 1,045 ℓ) Was used to add stress to the experimental animals at high temperature, and then the red ginseng extract of Example was administered to evaluate the protective effect against the high temperature stress of the red ginseng extract.

Specifically, the experimental animals were divided into three groups: 1) room temperature (24 ± 1 ° C) group (RT), 2) high temperature stress group (40 ± 1 ° C / 2h / day) , 3) high-temperature stress (40 ± 1 ℃ / 2h / day) group (HS + JP5), and 3) high temperature stress group was 100 mg / kg / day Or 2 mg at a concentration of 300 mg / kg / day (HS + JP5-H).

Behavioral observation was carried out for a total of 16 days.

The results of the initial 0 to 8 day behavioral observations are as described below.

- Stress induced by high temperature: about 30 minutes: 2) High temperature stress - induced group (HS) and 3) High temperature - stressed group (HS + JP5) Most experimental animals showed decreased activity such as crouching.

- About 30 minutes to about 1 hour: Aggressive behaviors such as drinking water and fighting each other were observed.

- Approximately 1 hour to about 1 hour and 30 minutes: Unlike the normal appearance of the prone type, most of the experimental animals have fallen in the abdomen toward the sky and have been shown to lean helplessly on the wall of the cage or to lose directionality and collapse.

- About 1 hour and 30 minutes to 2 hours: Not only increased drinking of water, but also watering from the jaw to the abdomen was observed, and in most of the hairs of the experimental animals, the glints disappeared and were observed to be rough and uneven.

(2) High temperature stress (HS) and (3) High temperature stress group (HS + JP5) after 1 hour of stress: 2) RT), which took about 2 hours to recover.

The results of the behavior observation for 8 to 16 days thereafter are as described below.

- 0 to 3 days: 2) High temperature stress group In the experimental animals (HS), most of the experimental animals collapsed in a short time after the start of the stress due to high temperature and collapsed after the end of the stress due to high temperature, 3) Some of the experimental animals (HS + JP5) treated with high temperature stress showed similar behaviors to those of experimental animals (HS) at high temperature.

- 4 ~ 7 days: 2) In the stress-untreated group (HS) by high temperature, most of the experimental animals showed a small amount of activity when stressed by high temperature and an increased time of being in a prone state before falling down, 3) Some of the experimental animals (HS + JP5) treated by high temperature showed similar behavior to those of experimental animals (HS) by high temperature. Were also observed.

(2) High-temperature stress-untreated group (HS) showed a cage showing intense behavior such as fighting against each other in addition to the increase in prone state time, 3) stress-induced stress group (HS + JP5 ) Were similar to those of experimental animals in the stress-untreated group (HS) at high temperature.

- Decrease in stress between the experimental animals of stress-untreated group (HS) and high-temperature stressed group (HS + JP5) due to high temperature, The difference of reaction intensity was observed clearly in time.

The result of the analysis of the volume of water is as shown in Fig.

As shown in FIG. 3, the experimental animals of the stress-induced group (HS + JP5-L and HS + JP5-H) It was predicted that the red ginseng extract of the example according to the present invention would rather reduce the stress due to high temperature and consequently improve the immunoregulatory ability deteriorated by the high temperature stress.

(2) Serum analysis

Male rats (SD), 7 weeks old, were purchased from Orient Bio Co., Ltd. as experimental animals, and after a week of adaptation period, they were placed in a high temperature environment unit of 260 mm × 800 mm × 1,830 mm size (volume: 1,045 ℓ) , The stress caused by the high temperature was added to the experimental animals for 4 weeks, and then the red ginseng extract of Example was administered to evaluate the efficacy of the red ginseng extract for eliminating immunity imbalance by stress.

Specifically, the experimental animals were divided into four groups: 1) 28 rats at room temperature (24 ± 1 ° C), 2) 14 rats without stress (40 ± 1 ° C / 2h / day) (3) High temperature stress (40 ± 1 ℃ / 2h / day) Low concentration (100mg / kg / day) group (HS + JP5-L) (HS + JP5-H) treated group at a high concentration (HS + JP5-L), and 100 mg / kg of the red ginseng extract of Example (HS + JP5-L) was orally administered at a concentration of 300 mg / kg / day in a concentration of 300 mg / kg / day for the high-stress group (HS + JP5-L) Blood samples were collected from orbital blood collected before and 1 week after administration of red ginseng extract and serum and plasma were separated and analyzed.

The amount of IL-2 and TNF-α secreted in the serum-immunized T-cell and antigen presenting cells (APC) and the secretion amount of IgA, IgM and IgE in the immunoglobulin secreted from B- And the results are as shown in Figs. 4A to 4E. When the immunoregulatory ability is deteriorated by high-temperature stress, cytokines IL-2 and TNF-α, and immunoglobulins IgA, IgM and IgE are further secreted to maintain homeostasis in the body.

On the other hand, secretion amounts of ACTH and Cortisol, which are typical high-temperature stress hormones in plasma, were measured, and the results are shown in FIGS. 5A and 5B. Under high-temperature stress, the secretion of stress-related hormones, ACTH and Cortisol, increases in the pituitary gland and adrenal glands in order to maintain homeostasis against stress.

As shown in Figs. 4 and 5, the experimental animals of the low-stress stress group (HS + JP5-L) by high temperature or the high stress group (HS + JP5-H) The secretion amount of cytokines IL-2 and TNF-α and the immunoglobulins IgA, IgM and IgE were markedly decreased, and the secretion amount of the hormone ACTH and cortisol was significantly reduced compared to the experimental animals of the HS It was confirmed that the red ginseng extract of the Example has an effect of reducing the high temperature stress and improving the immunoregulatory ability degraded by the high temperature stress.

While the present invention has been particularly shown and described with reference to preferred embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the following claims. . It is therefore to be understood that the modified embodiments are included in the technical scope of the present invention if they basically include elements of the claims of the present invention.

Claims (10)

Red ginseng extract having the effect of improving the immunoregulatory ability degraded by high temperature stress,
Ginsenosides Rb1, Rg3 (S), Rg2 (S) and Re,
Wherein the content of Rb1 is 1.74 to 2.166% by weight, the content of Rg3 (S) is 1.162 to 1.246% by weight, the content of Rg2 (S) is 0.497 to 0.565% by weight and the content of Re Is 0.109 to 0.177% by weight. The method according to claim 1,
Wherein the ginsenoside Rg1 is further contained, and the content of Rg1 is 0.16% by weight or less. 3. The method of claim 2,
Wherein the content of ginsenoside Rg1 is 0.05 to 0.16% by weight. 4. The method according to any one of claims 1 to 3,
Wherein the content of Rb1 is 1.80 to 2.166% by weight, the content of Rg3 (S) is 1.00 to 1.246% by weight, the content of Rg2 (S) is 0.50 to 0.565% by weight, and the content of Re Is 0.109 to 0.177% by weight. 4. The method according to any one of claims 1 to 3,
Wherein the total weight of ginsenosides is 3.554 to 5.513 wt% based on the total weight of the red ginseng extract. A health food comprising red ginseng extract according to any one of claims 1 to 3. Extracting red ginseng from water or ethyl alcohol,
Concentrating the supernatant of the precipitate after precipitation,
Sterilizing the concentrate, and
A method for producing the red ginseng extract according to any one of claims 1 to 3, comprising aging the concentrate at 70 to 110 DEG C for 40 to 56 hours. 8. The method of claim 7,
Wherein the step of aging the concentrate is performed at 90 to 110 캜 for 48 to 56 hours. 8. The method of claim 7,
Wherein the red ginseng extract is extracted with purified water corresponding to 8 times or more the weight of the raw red ginseng when the red ginseng is extracted with water. 8. The method of claim 7,
The precipitation is carried out at 8 to 10 DEG C for at least 12 hours,
Wherein the concentration is performed by first concentrating the supernatant at 30 or more Brix and secondarily concentrating the supernatant at a Brix of 70 or more.

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