KR20190058731A - Method of Cultivating Cassia Sprouts for increasing the antioxidant activity in Cassia Sprouts - Google Patents

Abstract

The present invention relates to a cultivation method of cassia sprouts, which increases the antioxidant activity of cassia sprouts using an LED as a light source when cultivating cassia sprouts. According to the present invention, it is possible to provide an eco-friendly and economical method for cultivating cassia sprouts in which antioxidant activity is enhanced, and it is easy to select light quality and control light quantity.

Description

[0001] The present invention relates to a method of cultivating a sprout vegetable for increasing the antioxidant activity,

The present invention relates to a method for cultivating a deficient sprout vegetable which increases antioxidant activity, and more particularly, to an antioxidative activity of a defatted sprout vegetable by increasing the antioxidative activity in a sprout vegetable by using an LED having a specific wavelength range as a light source Cultivation method.

The killer is a seed that brightens the eyes. It has a height of 1 to 1.5m, a leaf is 狀狀 birefringe, 2 to 4 pairs of obovate, and yellow in the armpit of July to August. Flowers bloom. A leaf-shaped pod opens about 10 cm behind the leaves. There is a line of glittering seeds in a pod, which is called a loser.

Seeds are 3 ~ 6㎜ long and columnar with 2 ~ 3.5㎜ diameter, and there are hairs and there are pale yellowish brown vertical lines and bands on both sides. The seeds have curved dark colored cotyledons. The country of origin is the United States and it is cultivated in various parts of Korea. The name "Kyo" is derived from the meaning that it brightens the eyes. It started from the "Divine Materia Medica".

The active ingredients include emodin, obtusifolin, obtusin, and the like. Animal experiments have shown that blood pressure reduction is effective, and experiments with toads have been shown to shrink blood vessels and to inhibit the development of staphylococci, E. coli and influenza.

Medicinal properties are cool, flavorful, and effective for treating hepatitis, used for ascites due to cirrhosis, and also effective for eye diseases caused by liver lesions. Therefore, it is used for the symptom of the eye congestion, the sunlight not visible or the tear. It is also used for acute conjunctivitis and corneal opacity.

In addition, diuretic and constipation is also effective for the treatment of habitual constipation, such as hypertension is known. Patients with hypotension are forbidden to use it, and are prohibited from using with hemp. As a typical prescription, Cucumisan is used for the eye. In addition, it is said that blood pressure decreases when it is roasted and drinks tea.

It is generally known that a loser is drenched mainly by tea, and in this case, the use of a roasted loser is more effective.

However, no studies on the antioxidative effect of the killer buds have been made so far. In particular, there has been no study on the specific cultivation method for increasing the antioxidant efficacy.

Korean Patent No. 0765436

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in view of the above problems, and it is an object of the present invention to provide a method for cultivating a defoliation sprout vegetable having enhanced antioxidative activity.

Another object of the present invention is to provide a cultivation method of a green and economical seedling sprout vegetable which is easy to select light quality and control light quantity.

In order to accomplish the above object, the present invention provides a method for cultivating a deficient sprout vegetable which uses an LED as a light source to increase the antioxidative activity of a defoliation sprout vegetable during the cultivation of a defrosted sprout vegetable.

In addition, the LED is characterized in that the antioxidative activity of the scallop vegetable is increased by using a wavelength in a specific range as a light source.

The specific wavelength range of the LED is 380 to 550 nm.

The specific wavelength range of the LED is 380 to 480 nm.

Further, the LED treatment of the sprouts vegetables is characterized by being performed in parallel with the cultivation of the cancer.

Further, the content of the free amino acid contained in the cleanser is increased by the LED in the wavelength range.

In addition, the antioxidant activity is characterized in that the antioxidative activity is obtained from an increase in the content of phenol or the content of vitamin C present in the scallop sprout vegetables.

According to the present invention, it is possible to provide a method for cultivating a defoliation sprout vegetable in which antioxidative activity is enhanced, and a method for cultivating a defoliation defoliation sprout vegetable which is easy to select light quality and control light quantity, and is environmentally friendly and economical.

The present invention relates to a cultivation method of a deficient sprout vegetable which increases the antioxidative activity of a defoliation sprout vegetable using an LED as a light source when cultivating a defrosted sprout vegetable.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

Cerebrates are known for their efficacy of clearing their eyes from the past, and they have been dumped by car in many households. In particular, when roasted seeds are used, the roasted seeds have been used in water dalyeoseo because the more savory flavor and flavor survive.

The present invention focuses on antioxidative efficacy among the effects of the catechins and finds a way to increase the antioxidant efficacy of the catechins. It has been found that the antioxidative effect can be increased by controlling the cultivation method Thereby completing the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

[Example]

[Comparative Example]

Treatment of cancer and fluorescence treatment

a. Plant Species: Culprit

b. Seed weight: 6 g / cell (3 repetitions)

c. Seed disinfection, washing and immersion: 10 mg / g of seeds were added to 20 mg / mL calcium hypochlorite, treated for 15 minutes, thoroughly washed in tap water and immersed in distilled water for 1 day.

d. Sowing: Sowing on plastic growers.

e. Cancer Treatment: Blinded for 6 days.

f. Fluorescent light treatment: treated with fluorescent light for 6 days (50 umol * m ^-2 * s ^-1 )

g. Cultivation temperature and humidity: 23 ± 2 ℃ (25/19) (maximum temperature / minimum temperature), 45 ± 5% (relative humidity)

h. Moisture supply: 150 ml / day of each cultivator was poured evenly over the beaker and replenished 3 to 4 times / day with spray, and the distilled water of the grower was replaced every 2 days.

i. Move the growing place during the cultivation period

[Experiment result]

The following Table 1 shows the results of comparing the content of free amino acids in the seedlings of the above-mentioned Comparative Example with the free amino acid content of seeds and roasted seeds.

Free amino acid Raw seed Roasted seed Cancer-grown bud Light-grown bud Ser 7.14 5.56 52.59 45.58 Arg 20.32 10 161.53 92.04 Gly 7.33 2.37 32.04 28.07 Asp 14.53 6.25 55.03 57.29 Glu 24.58 10.67 55.86 68.15 Thr 8.58 3.3 52.99 39.22 Ala 9.62 0 54.82 54.27 Pro 10.86 0 69.83 70.53 Lys 17.74 0 62.94 58.03 Tyr 0 0 22.74 21.8 Met 0 0 210.25 200.26 Val 6.54 0 4.49 2.68 Ile 9.86 0 60.04 35.3 Leu 21.76 0 107.62 83.85 Phe 14.93 0 62.47 48.66 Total 173.8 38.14 1065.22 905.73

(Unit: mg / 100g building)

As can be seen from the results of Table 1, it can be seen that the free amino acid content of the cultivated shoots or light cultivated shoots was significantly higher than that of the seeds or roasted seeds in the type of the cuttlefish.

Table 2 shows the contents of vitamin C and total phenol compounds in the seedlings prepared by cultivation of buds treated with cancer-treated buds or fluorescent lamps of the comparative example in comparison with the contents of vitamin C and total phenol compounds in the seeds and roasted seeds .

Raw seed Roasted seed Cancer-grown bud Light-grown bud Vitamin C 0.08 0.05 11.54 13.43 Total phenolic compound 20.37 13.89 30.66 34.77

(Unit: mg / g building)

As can be seen from the results in Table 2, the content of vitamin C and total phenolic compounds in cancer-grown buds and light-grown buds was significantly higher than that of seeds or roasted seeds.

Table 3 shows the results of DPPH radical scavenging activity of the seedlings prepared by cultivating the seedlings grown in the cancer-treated buds or the fluorescent-treated buds of the comparative example, compared with DPPH radical scavenging activity of the seeds and roasted seeds.

Raw seed Roasted seed Cancer-grown bud Light-grown bud 50 μg / mL 20.19 20.8 23.69 23.67 100 μg / mL 28.79 23.87 30.55 31.65 200 μg / mL 40.07 38.92 40.48 52.84 400 μg / mL 51.41 51.07 59.65 74.87 IC ₅₀ (μg / mL) 369.75 372.72 299.9 210.94

(unit: %)

As can be seen from the results in the above Table 3, the DPPH radical scavenging activity was increased in cancer-grown buds or light-grown buds rather than seed or roasted seeds.

[Example]

LED treated cutter sprout vegetable growing

a. Plant Species: Culprit

b. Seed weight: 6 g / cell (3 repetitions)

c. Seed disinfection, washing and immersion: 10 mg / g of seeds were added to 20 mg / mL calcium hypochlorite, treated for 15 minutes, thoroughly washed in tap water and immersed in distilled water for 1 day.

d. Sowing: Sowing on plastic growers.

e. Cancer treatment: blinded for 2 days.

f. LED treatment: 2 days after cancer treatment and 4 days with LED treatment.

(C: Fluorescent lamp, T1: UV (385), T2: Blue (465), T3: Red (645), T4: IR

Treatment C T1 T2 T3 T4  Wavelength (nm) Fluorescent lamp 385 465 645 780

g. Light intensity: 50 umol * m ^-2 * s ^-1

h. Cultivation temperature and humidity: 23 ± 2 ℃ (25/19) (maximum temperature / minimum temperature), 45 ± 5% (relative humidity)

i. Moisture supply: 150 ml / day of each cultivator was poured evenly over the beaker, replenished 3 to 4 times per day, and the distilled water of the grower was replaced every 2 days.

j. Move the growing place during the cultivation period

[Experiment result]

The following Table 5 shows the results of measurement of the free amino acid content of the killer buds prepared by cancer treatment and LED treatment bud cultivation of the above-mentioned Examples.

Free amino acid Fluorescent lamp 385 nm 465 nm 645 nm 780 nm Ser 74.23 81.41 93.82 76.28 50.56 Arg 184.55 235.84 217.27 189.14 190.2 Gly 48.95 42.86 54.7 47.44 35.98 Asp 53.2 68.81 49.66 43.4 45.88 Glu 77.24 78.18 82.83 82.76 68.4 Thr 67.45 81.6 84.38 71.24 73 Ala 71.72 76.68 86.86 72.57 79.36 Pro 90.02 93.81 105.42 83.76 92.21 Lys 94.64 81.42 96.82 94.85 70.98 Tyr 28.42 55.34 101.18 95.53 25.99 Met 198.25 197.58 191.56 233.37 215.76 Val 19.98 18.24 20.35 21.88 18.91 Ile 63.14 81.54 62.9 72.34 83.47 Leu 114.65 146.72 154.22 157.37 146.78 Phe 99.84 95.72 100.12 109.95 97.78 Total 1286.28 1435.75 1502.09 1451.88 1295.26

(Unit: mg / 100 g building)

Table 6 shows the results of measuring the contents of vitamin C and total phenolic compounds in the killer buds prepared by the cancer treatment and the LED treatment bud cultivation of the above example.

Fluorescent lamp 385 nm 465 nm 645 nm 780 nm Vitamin C 13.24 13.79 15.62 11.35 11.2 Total phenolic compound 36.81 38.48 40.56 36.48 32.05

(Unit: mg / g building)

Table 7 shows the results of measuring DPPH radical scavenging activity of the killer buds prepared by cancer treatment and LED treatment bud cultivation of the above example.

Light treatment wavelength Fluorescent lamp 385 nm 465 nm 645 nm 780 nm 50 μg / mL 18.02 22.13 24.42 20.38 18.54 100 μg / mL 32.78 32.89 34.58 30.05 29.88 200 μg / mL 47.66 49.72 51.46 42.76 42.76 400 μg / mL 73.29 76.45 80.61 66.72 63.4 IC ₅₀ (μg / mL) 234.39 218.37 201.54 265.17 279.87

(unit: %)

As a result of comparing the experimental results of the examples and the comparative examples, it was found that the content of the free amino acids was increased and the content of the total amino acids was increased compared to the contents of the amino acids of the cancer-grown buds and the light- (Table 1 and Table 5).

As a result of comparing the data of Tables 2 and 3 with the data of Tables 6 and 7, it was found that the content of vitamin C and total phenol compounds and DPPH radical scavenging activity were also significantly increased. Thus, The antioxidant efficacy of the killer was increased.

In addition, the increase of the free amino acid content and the increase of the antioxidant activity are slightly different even in the wavelength of the processed LED light, and the increase effect is particularly large at the wavelengths of 385 nm and 465 nm.

Therefore, the range of the LED wavelength used in the cultivation method of the decanter sprouts of the present invention is preferably from 380 to 550 nm, particularly preferably from 380 to 480 nm.

Claims (7)

A method of cultivating a sprout vegetable that increases the antioxidant activity of a defoliation sprout vegetable using an LED as a light source when cultivating a sprout vegetable.
The method according to claim 1,
Wherein the LED is used as a light source in a specific range of wavelength to increase the antioxidant activity of the killer sprouts vegetables.
3. The method of claim 2,
Wherein the specific wavelength range of the LED is 380 to 550 nm.
The method of claim 3,
Wherein the specific wavelength range of the LED is 380 to 480 nm.
5. The method according to any one of claims 1 to 4,
Wherein the LED treatment of the sprout vegetable is performed in parallel with the cultivation of the cancer.
The method according to claim 1,
Wherein the content of the free amino acid contained in the cleanser is increased by the LED in the wavelength range.
The method according to claim 1,
Wherein the antioxidant activity is obtained from an increase in the content of phenol or vitamin C present in the scallop sprout vegetables.