KR20190053488A - Cosmetic composition for skin whitening comprising the extract of Silene seoulensis Nakai as active ingredient - Google Patents

Abstract

The present invention relates to a skin-whitening cosmetic composition containing a Silene seoulensis Nakai extract as an active ingredient. More particularly, the present invention relates to a skin-whitening cosmetic composition which contains an ethanol extract of an aerial part of Silene seoulensis Nakai as an active ingredient to inhibit tyrosinase activity and suppress melanin pigmentation.

Description

[0001] The present invention relates to a cosmetic composition for skin whitening,

The present invention relates to a method for producing a long- The present invention relates to a cosmetic composition for skin whitening, which comprises an extract of ethanol as an active ingredient and a skin whitening agent which inhibits tyrosinase activity and inhibits the deposition of melanin pigment And more particularly to a cosmetic composition for cosmetics.

As human life becomes more diverse, the demand for white and clean skin increases day by day, and the market for whitening cosmetics is growing day by day, and more and more attention is being paid to its efficacy, and as its functionality is emphasized, The tendency to obtain from friendly and natural is increasing, and the demand for the use and study of natural raw materials is getting stronger.

The skin color of a person is determined by the amount of melanin, carotene and hemoglobin, among which melanin has the greatest effect and is also influenced by skin thickness, reflectance and blood flow. The melanin pigment, which is the most important factor for determining skin color, is a tyrosinase that is an enzyme contained in melanocytes existing in the basal layer of the epidermis, tyrosine, which is a type of amino acid normally present in the human body. dopa), followed by a series of complicated oxidation processes to finally produce melanin, a dark brown polymer.

Tyrosinase is a metal-containing protein that is distributed in almost all species such as microorganisms, plants and animals, and contains a pair of copper ions in its active site. The function of tyrosinase is closely related to skin-related diseases, Are mainly focused on the development of an inhibitor of tyrosinase activity. In animals, abnormal melanin staining, such as vitiligo and pigmentation, is caused by abnormalities in the synthesis of melanin, which can cause skin cancer in severe cases.

Currently known methods for whitening include sunscreening, signal suppression, suppression of gene expression, inhibition of enzyme action, pigment reduction and stratum corneum removal. Arbutin, kojic acid, ascorbic acid, and hydroquinone have been used as a representative single substance exhibiting whitening function, and natural materials for the development of natural whitening agents are actively being explored.

Silene seoulensis Nakai is an annual plant of Korean specialty, which belongs to Caryophyllaceae and grows in the mountains and shadows of mountains all over Korea except Jeju Island. The stem is 30-60cm high, the bottom is to the side, the upper part grows straight and has a downward hairs. Leaves are opposite, ovate, pointed end. The flower runs in June to August at the end of the stem and has a white or reddish white color with a diameter of 1.2cm. The peduncle is thin and long. The petals are divided into 5 pieces and the ends are divided into 2 branches. Fruit is capsule, ovate. It is also distributed in Japan and China. In addition, the leaves are ovate, petiole, and the lower part of the stem grows as compared to the silane plants in Korea. There are few reports on active substances and physiological activities of elderly goats.

The present invention relates to a method for treating skin diseases, seoulensis The present invention relates to a cosmetic composition for skin whitening, which contains an extract of Nakai as an active ingredient. Since no whitening effect has been reported for the extract of long-chitosan extract, it has been confirmed through inhibition of melanin production in melanin cells of an extract using an organic solvent such as ethanol , And it can be usefully used as skin whitening cosmetic or whitening agent since it has no cytotoxicity.

An object of the present invention is to develop a skin whitening cosmetic composition containing an active ingredient having excellent whitening efficacy and having no skin irritation.

The present invention provides a cosmetic composition for skin whitening comprising an elongated tobacco extract as an active ingredient.

The present invention provides a cosmetic composition for skin whitening comprising a fine longchain extract in an amount of 0.001 to 20% by weight based on the total weight of the cosmetic composition.

According to the present invention, the present invention is not only superior in skin whitening effect but also widely used in the cosmetics industry since it is a plant-derived extract.

Fig. 1 shows the effect of inhibiting tyrosinase activity by the extract of the long-chitosan.
Fig. 2 shows the cytotoxicity of melanoma cells of the elongated sweet potato extract.
FIG. 3 shows the effect of the elongated tobacco extract on the inhibition of thiosinase mRNA synthesis in melanoma cells.

Hereinafter, the present invention will be described in detail.

The inventors of the present invention conducted research to develop a natural raw material having a skin whitening effect with excellent stability without inducing skin side effects. As a result, it was confirmed that a fine longchyme extract inhibited melanin production and had a skin whitening effect Thereby completing the present invention.

The present invention provides a cosmetic composition for skin whitening comprising a fine longchild extract.

The present invention relates to a cosmetic composition for skin whitening, which comprises an extract obtained by using a ground part of a fine long bean jam.

Silene seoulensis Nakai is an annual plant of Korean specialty, which belongs to Caryophyllaceae and grows in the mountains and shadows of mountains all over Korea except Jeju Island. The stem is 30-60cm high, the bottom is to the side, the upper part grows straight and has a downward hairs. Leaves are opposite, ovate, pointed end. The flower runs in June to August at the end of the stem and has a white or reddish white color with a diameter of 1.2cm. The peduncle is thin and long. The petals are divided into 5 pieces and the ends are divided into 2 branches. Fruit is capsule, ovate. It is also distributed in Japan and China. In addition, the leaves are ovate, petiole, and the lower part of the stem grows as compared to the silane plants in Korea.

The present invention relates to a cosmetic composition for skin whitening, which comprises an active ingredient extracted from a liquid cosmetic composition using a liquid solvent, and the solvent includes distilled water, 1,3-butylene glycol, And acetone, or a mixture thereof is preferably used. As the solvent, an alcohol having 1 to 4 carbon atoms is particularly preferable.

In order to efficiently extract the active ingredient, the alcohol having 1 to 4 carbon atoms is preferably extracted using methanol or ethanol or a mixture thereof. However, the alcohol having relatively high boiling point and relatively toxic It is most preferred to use less ethanol.

In addition, the concentration of the alcohol having 1 to 4 carbon atoms is not particularly limited, but it is preferable to use a concentration of 10 to 90%. In addition, if the above-mentioned alcohol having 1 to 4 carbon atoms is used at a concentration of less than 10%, there may arise a problem that the active ingredient can not be extracted sufficiently from the long sweet potatoes. If the alcohol is used at a concentration exceeding 90% It is most preferable to use an alcohol having a carbon number of 1 to 4 at a concentration within the above range because the active ingredient to be used may be deformed or damaged.

In addition, the method for extracting the fine longchild extract may be any one of room temperature extraction, hot water extraction, cold extraction, reflux cooling extraction, ultrasonic extraction or steam extraction. The extraction method used in the present invention is a conventionally used extraction method, and a detailed description thereof will be omitted.

The cosmetic composition for skin whitening of the present invention contains a fine longchild extract in an amount of 0.001 to 20% by weight based on the weight of the whole cosmetic composition.

The cosmetic composition for whitening skin of the present invention may contain 0.001 to 20% by weight of a fine gypsum extract, but the content thereof can be appropriately adjusted depending on the content of the other components contained in the formulation or cosmetic composition. However, if the content of the extract is less than 0.001% by weight, it may be difficult to expect a substantial whitening effect in the cosmetic composition, and if it exceeds 20% by weight, the cosmetic composition may be uneconomical to the effect of cost increase. Preferably, the present invention includes 0.01 to 5.0% by weight of a fine long-cherry pickle. The cosmetic composition may further contain all kinds of ingredients which can be used in ordinary cosmetics, such as excipients, diluents, etc., in addition to the fine long-horn extract.

The skin whitening cosmetic composition of the present invention may be prepared in any formulations conventionally produced in the art, and examples thereof include a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, But are not limited to, cleansing, oils, powder foundations, emulsion foundations, wax foundations, packs, massage creams and sprays. More specifically, it can be prepared in the form of a soft lotion, a nutritional lotion, an essence, a nutritional cream, a massage cream, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the skin whitening cosmetic composition of the present invention is a paste, a cream or a gel, the carrier component may be selected from vegetable oils, animal oils, waxes, paraffins, starches, tragacanth, cellulose derivatives, silicones, bentonites, polyethylene glycols, Talc or zinc oxide may be used.

When the formulation of the skin whitening cosmetic composition of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl acetate, ethyl carbonate, benzyl Alcohol, benzyl benzoate, propylene glycol, polyethylene glycol, 1,3-butylene glycol oil, glycerol aliphatic ester, or fatty acid esters of sorbitan can be used.

When the formulation of the skin whitening cosmetic composition of the present invention is a suspension, it is preferable to use a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like may be used.

When the formulation of the skin whitening cosmetic composition of the present invention is a powder or a spray, lactose, silica, talc, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component, Propellants such as chlorofluorohydrocarbons, propane, butane or dimethyl ether.

When the formulation of the skin whitening cosmetic composition of the present invention is a cleansing agent containing a surfactant, it is preferable to use, as the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, sarcosinate, Alkylamido betaines, aliphatic alcohols, fatty acid amide ether sulfates, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the cosmetic composition for skin whitening of the present invention is a cleansing formulation containing a surfactant or a cleansing formulation without a surfactant or a soap, it may be applied to the skin and then wiped off or washed off with water. The surfactant-free cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

The skin whitening cosmetic composition of the present invention can be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and may be used in a different number of times depending on the skin condition or taste of the user.

Hereinafter, the present invention will be described in more detail based on preferred embodiments of the present invention. However, the present invention is not limited to such an embodiment.

Example

< Manufacturing example >

450 g of the long sweet potatoes were crushed and mixed with 10 times by weight of 70% ethanol. The mixture was allowed to stand at room temperature for 24 hours, repeatedly extracted twice and filtered. The filtrate was concentrated under reduced pressure and lyophilized to obtain 51 g of solid, .

< Experimental Example 1> Determination of inhibitory effect on the activity of tyrosinase activity

In order to confirm the whitening effect of elongated tofu extract, it was tested whether the activity of tyrosinase, which plays an important role in the formation of melanin, is inhibited by the intestinal extract.

10 mM L-3,4-dihydroxyphenylalanine (L-DOPA) (Sigma, USA) was diluted in sodium phosphate buffer (0.1M pH 6.8) and used for the experiment. Mushroom tyrosinase Sigma, USA) was used at a concentration of 40 units / mL. Samples were diluted in sodium phosphate buffer at 10, 100, 500, and 1000 μg / mL, and 60 μL of each sample was added to a 96-well plate. To each sample was added 20 μL of L-DAPA and 20 μL of mushroom tyrosinase. DOPA chrome produced in the reaction solution was measured with a microspectrophotometer (Molecular Device, Sunnyvale, Calif., USA) at a wavelength of 492 nm. As a control, the enzyme activity was measured by adding distilled water instead of the sample. Tyrosinase inhibition rates were calculated using the following formula.

Tyrosinase inhibition rate (%) = {1- (S-B) / C} X 100

S: absorbance at the time of addition of enzyme solution and sample solution

B: Absorbance at the time of addition of buffer instead of enzyme solution

C: Absorbance at the time of adding the buffer solution instead of the sample solution

As a result of treatment with 10, 100, 500 and 1000 μg / ml of Ganoderma lucidum extract on the basis of the activity of untreated tyrosinase, 10.4 ± 0.9, 23.8 ± 2.3, 51.8 ± 0.2 , And 62.0 ± 0.3%, respectively.

& Lt; Experimental Example 2 > Evaluation of Toxicity of Extracts of Melanoma Extracts to Melanoma Cells

In order to evaluate toxicity of melanoma cells expressing skin pigment, the extracts were treated with melanoma cell line B16F10 cells and cell viability was analyzed by MTT assay.

B16F10 cells were purchased from Korean Cell Line Bank (KTCC, Seoul, Korea) and were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 100 units / ml penicillin / streptomycin and 10% fetal bovine serum ) (Gibco, USA) medium at 37 ° C in a 5% CO ₂ incubator. B16F10 cells were plated in 96-wells at ⁵ x ¹⁰ cells / ml and stabilized for 24 hours. Samples were treated at different concentrations and cultured in a CO ₂ incubator for 24 hours. After incubation for 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide and handle (MTT, 0.5 mg / ml) (Sigma, USA) solution, CO ₂ 4 hours incubation After incubation for 30 min at 37 ° C, DMSO was added and analyzed with a microspectrophotometer (Molecular Device, Sunnyvale, Calif., USA) at a wavelength of 540 nm.

The results were as follows: 1.25, 2.5, 5, 10, 20, 40, 60, and 100 μg / ml of the extracts of Bombyx mori L. on the melanoma cell line B16F10 were treated at a concentration of 1.25 and 2.5 μg / The cell viability was decreased by -10%, but it was not statistically significant. The cell viability was about 100% even at a concentration of 100 μg / ml, confirming that no toxicity was observed.

< Experimental Example 3> Determination of inhibitory effect on the production of tyrosinase mRNA in melanoma cells

In order to confirm the whitening effect of elongated tofu extract, it was confirmed that the activity of tyrosinase, which plays an important role in the formation of melanin, is inhibited by the elongated tobacco extract.

B16F10 cells were seeded at 5 × 10 ⁵ cells / ml in a 6-well plate and cultured. After 24 hours, the extracts were incubated for 1 hour and α-melanocyte stimulating hormone (α-MSH) (Sigma, USA) was added at a concentration of 400 nM for 24 hours. MRNA was extracted using Trizol Reagent (Life Technologies, Carlsbad, CA, USA) and cDNA was synthesized using PrimeScript RT reagent kit (TaKaRa, Shiga, Japan). PCR-PreMix (Bioneer, Korea) was used for PCR amplification. 26 cycles of 30 sec at 95 ° C, 30 sec at 62 ° C, and 30 sec at 72 ° C were repeated and electrophoresed on 1% agarose gel. The primers used in the PCR are as follows. Tyrosinase positive direction; TGG GAA AAA CGT CTG CGA TG, tyrosinase reverse direction; CAC GGA CCT CCA AGC ACT GA, GAPDH Forward; TGA CCA CAG TCC ATG CCA TC, GAPDH reverse direction; TTA CTC CTT GGA GGC CAT GT.

As a result, α-MSH treatment of melanoma cells increased mRNA production of tyrosinase compared with naive group. At the concentrations of 1 and 10 μg / ml, the results showed that the elongated leaves slightly decreased the expression of tyrosinase. However, when cells were treated with 50 μg / ml and 100 μg / ml of extract, the production of tyrosinase mRNA, which was increased by α-MSH, decreased in a concentration-dependent manner.

Claims (5)

Silene seoulensis Nakai) extract. The method according to claim 1,
The cosmetic composition for whitening skin according to claim 1, wherein the fine gourd extract is a ground-part extract of a fine gourd. The method according to claim 1,
The composition for skin whitening cosmetics according to any one of claims 1 to 3, wherein the fine longchyme extract is contained in an amount of 0.001 to 20% by weight based on the total weight of the cosmetic composition. The method according to claim 1,
The cosmetic composition for skin whitening as set forth in claim 1, wherein the fine gypsum extract is extracted with at least one solvent selected from the group consisting of distilled water, 1,3-butylene glycol, alcohol having 1 to 4 carbon atoms, ethyl acetate and acetone. The method according to claim 1,
The cosmetic composition may be in the form of an ointment, essence, lotion, cream, gel, lotion, pack, massage cream, milky lotion, foundation, makeup base, soap, liquid cleanser, bath agent, sunscreen, cleansing foam, cleansing lotion and sun oil Lt; RTI ID = 0.0 &gt; 1, &lt; / RTI &gt; or a pharmaceutically acceptable salt thereof.

Images