KR20190048733A - In-vitro diagnostic system comprising attaching type-optical device using mobile device - Google Patents

Abstract

The present invention relates to an in-vitro diagnostic system having an attachable optical system using a mobile device. More specifically, the in-vitro diagnostic system comprises: a strip sensor to detect a biomaterial; an attachable optical system including a support unit to support the strip sensor, a fluorescent filter arranged on an upper portion of the support unit, a lens, and a light source; and a mobile device which includes a camera and an application to distinguish the biomaterial in an image photographing the strip sensor by the camera, and is capable of wireless communication with the attachable optical system. According to the present invention, a fluorescent signal of an LFA strip can be measured by a small and simple structure and low power. A wireless internet function of the mobile device is used to effectively transmit diagnosis results with information such as test location information and date and time to a central control server to realize a device and a system for virus diagnosis for field inspection based on personal portable mobile. Also, the system is suitable for quick and low-price inspection based on an LFA strip. A reader includes a separate LFA strip reader, the small attachable optical system attached to the camera of the mobile device, and the mobile device to realize a small low-price system. LFA inspection diagnosis results can be quickly and accurately linked to a server.

Description

TECHNICAL FIELD [0001] The present invention relates to an in vitro diagnostic system having an attachment type optical system using a mobile device,

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an extracorporeal diagnostic system having an attachment type optical system using a mobile device, and more particularly to an extracorporeal diagnostic system including an attachment type optical system using a mobile device for virus diagnosis.

Conventional biodiagnostic technology, especially acute viral immunodiagnostic sensor technology, is a portable strip reader composed of an LFA (lateral flow assay) -based immunodiagnostic strip and a separate optical system for reading the result, As shown in FIG. Since the measurement value of the reader is stored in the memory of the device and the measurement result is transmitted using the storage device or the wired / wireless communication, since a separate gateway or communication module is utilized, there is a disadvantage to apply to the emergency control and central control through the field diagnosis there was.

If the results of biochemical tests such as acute viruses or infectious and seasonal diseases are automatically stored in a central server through the central control system, The device has a disadvantage that it is difficult to cope with it.

In addition, it is required to implement a low-cost system because LFA strip readers should be used.

As a related art, Korean Patent Laid-Open Publication No. 2004-0102726 (entitled "Mobile diagnostic method and system using a PDA and a bio-signal measuring device") is provided with a bio-signal detecting means for detecting a bio- And a method of displaying a treatment method according to a diagnosis based on a stored rule rule based on a living body signal from the living body signal sensing means. However, the bio-signals measured in the preceding patent are blood pressure, blood glucose level, Pulse rate, body temperature, electrocardiogram and the like, and does not disclose a configuration in which an LFA (lateral flow assay) -based immunodiagnostic strip is combined with a mobile diagnostic device.

Korean Patent Publication No. 2004-0102726 (2004.12.08)

Accordingly, an object of the present invention is to provide a mobile optical device, which is composed of a hardware / program of a mobile device and an optical system with a mobile device attachment, and has an attachment type optical system using a mobile device capable of effectively transmitting a fluorescence signal measurement result of an LFA strip with a small simple structure and low power The present invention provides an in vitro diagnostic system.

It is another object of the present invention to provide an optical system for a mobile device which is equipped with a small attachable optical system attached to a camera of a mobile device and a reader equipped with a mobile device, Diagnosis system.

In order to achieve the above object, an in vitro diagnostic system including an attachment type optical system using a mobile device of the present invention comprises: a strip sensor for detecting a biomaterial; An attachment type optical system including a support for supporting the strip sensor, a fluorescent filter disposed on the support, a lens, and a light source; And a mobile device capable of wireless communication with the attachment type optical system, comprising a camera, and an application for discriminating the biomaterial from the image photographed by the camera with the strip sensor.

In addition, in the in vitro diagnostic system having the attachment type optical system using the mobile device of the present invention, the attachment type optical system may further include: a support part for supporting the strip sensor; A fluorescent filter disposed on an upper portion of the strip sensor window; A lens disposed on top of the fluorescence filter; And a light source disposed on left and right sides of the fluorescent filter.

In addition, in the in vitro diagnostic system having the attachment type optical system using the mobile device of the present invention, the attachment type optical system includes a black-out sheet on the upper surface of the strip sensor.

In addition, in the in-vitro diagnostic system having the attachment type optical system using the mobile device of the present invention, the wireless communication is Bluetooth communication.

In addition, in the in vitro diagnostic system having the attachment type optical system using the mobile device of the present invention, the mobile device may further include: an application execution unit that executes the application; A control unit for controlling the light source of the optical system and turning it on when the application is executed; An image capturing and processing unit for capturing the strip sensor continuously or step by step using a camera provided in the mobile device while the application is executed; An image analysis and determination unit for analyzing the photographed image to determine a biomarker concentration; And a judgment information display section for displaying judgment results of the image analysis and judgment section.

In addition, in the in vitro diagnostic system having the attachment type optical system using the mobile device of the present invention, the attachment type optical system further includes a drive circuit portion in the light source.

In addition, in the in vitro diagnostic system having the attachment type optical system using the mobile device of the present invention, in order to perform the in vitro diagnosis through the in vitro diagnostic system including the attachment type optical system using the mobile device, And the image capturing and processing unit controls the image capturing and processing unit so that the strip sensor is continuously or step-by-step performed by using a camera provided in the mobile device in a state in which the application is executed while power is applied to the light source through the driving circuit unit of the attaching- And the image analysis and judgment unit analyzes the photographed image to discriminate the biomarkers.

In addition, in the in vitro diagnostic system having the attachment type optical system using the mobile device of the present invention, the strip sensor is a strip for acute virus diagnosis or acute myocardial infarction diagnosis.

In addition, in the in vitro diagnostic system having the attachment type optical system using the mobile device of the present invention, the strip sensor is an LFA strip using a fluorescent substance as a label.

In addition, in the in vitro diagnostic system having an attachment type optical system using a mobile device of the present invention, the light source of the optical system is disposed on both sides or in four directions of the strip sensor window to uniformly illuminate the window of the strip sensor .

The in vitro diagnostic system having an attachment type optical system using a mobile device according to the present invention is composed of a mobile device camera, a hardware / program of a mobile device, and a mobile device attachment type optical system, It is possible to transmit effectively by using the wireless Internet function of mobile device together with the information of the location information and the date and time that the diagnosis result is checked on the central control server, System implementation is possible.

The attachment type optical system attached to the front end of the mobile device camera comprises an LED light source suitable for LFA fluorescence measurement, an optical system composed of a lens and a filter, a jig for fixing the position of the LFA, and a drive circuit for driving the LED light source, It is suitable for quick price inspection based on speed, and it is possible to implement a small price system by using a separate LFA strip reader, a small attachment type optical system attached to a mobile device camera and a mobile device without hardware / software, And the ease with which the diagnosis result can be linked to the server is excellent.

In addition, the LFA reader, which consists of a mobile device camera, an attachment type optical system, and a mobile application program, is a small, low cost system. It is an application program that controls the camera of a mobile device. It is advantageous to implement the analyzer system.

1 is a block diagram of a mobile device-based LFA strip sensor reader system according to the present invention (comprising an LFA strip, an attachment type optical system, a mobile device, and a driving program, and the optical system includes a circuit for driving an LED light source)
2 is a structural view of an attachment type optical system according to the present invention.
3 is a photograph showing an improvement effect of a measurement image by the built-in processing of a black-out sheet in an attachment type optical system according to the present invention.
4 is an operation algorithm of a mobile device based LFA strip reader system according to the present invention.
FIG. 5 is a graph showing a result of an operation algorithm of the mobile device image analysis program according to the present invention and an image processing result of each operation.
FIG. 6 is an embodiment showing a GUI configuration of a mobile device application program according to the present invention.
FIG. 7 shows an image and analysis result of an LFA strip reading result using a dengue virus sample according to the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, wherein like reference numerals are used to designate identical or similar elements, and redundant description thereof will be omitted. The suffix " module " and " part " for the components used in the following description are given or mixed in consideration of ease of specification, and do not have their own meaning or role. In the following description of the embodiments of the present invention, a detailed description of related arts will be omitted when it is determined that the gist of the embodiments disclosed herein may be blurred. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to provide further explanation of the invention as claimed. , ≪ / RTI > equivalents, and alternatives.

Terms including ordinals, such as first, second, etc., may be used to describe various elements, but the elements are not limited to these terms. The terms are used only for the purpose of distinguishing one component from another.

It is to be understood that when an element is referred to as being "connected" or "connected" to another element, it may be directly connected or connected to the other element, . On the other hand, when an element is referred to as being "directly connected" or "directly connected" to another element, it should be understood that there are no other elements in between.

The singular expressions include plural expressions unless the context clearly dictates otherwise.

In the present application, the terms "comprises", "having", and the like are used to specify that a feature, a number, a step, an operation, an element, a component, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. It will be apparent to those skilled in the art that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof.

An extracorporeal diagnostic system having an attachment type optical system using a mobile device of the present invention comprises: a strip sensor for detecting a biomaterial; An attachment type optical system including a support for supporting the strip sensor, a fluorescent filter disposed on the support, a lens, and a light source; And a mobile device capable of wireless communication with the attachment type optical system, comprising a camera, and an application for discriminating the biomaterial from the image photographed by the camera with the strip sensor.

Here, the attachment type optical system may include: a supporting part for supporting the strip sensor; A fluorescent filter disposed on an upper portion of the strip sensor window; A lens disposed on top of the fluorescence filter; And a light source disposed on left and right sides of the fluorescent filter. At this time, the light source further includes a driving circuit unit. Preferably, the light source of the optical system is disposed on both sides or in four directions of the strip sensor window to uniformly irradiate the window of the strip sensor.

In addition, the attachment type optical system includes a black-out sheet on the upper surface of the strip sensor.

The wireless communication may be a Bluetooth communication.

The mobile device may further include: an application execution unit that executes the application; A control unit for controlling the light source of the optical system and turning it on when the application is executed; An image capturing and processing unit for capturing the strip sensor continuously or step by step using a camera provided in the mobile device while the application is executed; An image analysis and determination unit for analyzing the photographed image to determine a biomarker concentration; And a judgment information display section for displaying judgment results of the image analysis and judgment section.

1, the configuration of the on-site diagnostic virus measurement system based on the mobile device provided by the present invention includes an LFA strip sensor, a mobile device and a built-in application program for a mobile device, . As shown in Fig. 2, the attachment type optical system can be composed of a lens, a fluorescence filter, a light source LED, an LFA strip and an optical mechanism structure portion for supporting the position thereof. At this time, it is preferable that the optical axis of the camera of the mobile device and the optical axis of the lens of the attachment type optical system are located on the same line. The method and apparatus for attaching to the mobile device are not particularly limited and include all of those widely known in the art.

In addition, the upper surface of the LFA strip is made up of a black-out sheet layer and an interior-treated surface that significantly reduce light diffusing in all areas except the measurement window. In Fig. 2, the outer case of the attachment type optical system is omitted. As shown in FIG. 3, the use of a black-out sheet on the upper surface of the LFA strip has an advantage of remarkably improving the contrast of the fluorescent image of the LFA strip. The black-out sheet is preferably a black-out blind (trade name) or the like as a plastic component made of a polymer material having a property of blocking light transmission.

Meanwhile, in the in vitro diagnostic system including the attachment type optical system using the mobile device of the present invention, in order to perform the in vitro diagnosis through the in vitro diagnostic system having the attachment type optical system using the mobile device, And the image capturing and processing unit controls the image capturing and processing unit so that the strip sensor is continuously or step-by-step performed by using a camera provided in the mobile device in a state in which the application is executed while power is applied to the light source through the driving circuit unit of the attaching- And the image analysis and judgment unit analyzes the photographed image to discriminate the biomarkers.

At this time, the strip sensor is preferably a strip for acute viral diagnosis or acute myocardial infarction diagnosis. In addition, the strip sensor may be an LFA strip using a fluorescent material as a label.

Referring to FIG. 4, FIG. 4 illustrates an operation algorithm of an entire program for controlling an application program implemented in a mobile device and a circuit for controlling an LED light source of an attachment type optical system. The mobile device and the LED driving circuit are shown to operate in conjunction with wireless communication using Bluetooth low energy (BLE), and it is also possible to control by a serial connection using a cable. The main operation sequence of the application program is to start the interlocking with the LED circuit with the low power Bluetooth (BLE), control the camera of the mobile device to complete the setting, transmit the LED light amount information and on / off control information to the circuit, check the operation, And analyzing and storing them.

5 illustrates an algorithm for operating a camera of a mobile device to obtain and analyze an image. It is obtained by photographing three photos continuously. The image is cropped to a desired window area, and unnecessary parts are removed to reduce burden on the image processing. Then, the image is converted into gray information and merged to produce a single image. The Sobel algorithm, which automatically detects the position of the line where the fluorescence signal is derived from the virus detection immunoreaction on the LFA strip, determines the analysis position by performing outline processing with a large inter-pixel contrast ratio of the image. Since the size of the control signal is large in the control region, the image is clearly displayed so that the contrast ratio can be easily analyzed. Therefore, the position of the actual test region can be determined based on the position of the control region. Because the test area is very low, we analyze the physical distance information between control / test lines in LFA strip manufacturing using image analysis. The spacing between the two lines in the manufacture of the LFA strip can be made constant.

The ratio of the fluorescence signal values on the two areas of the control and the test line is calculated and converted to the concentration of the target substance by comparing with the quantitative curve data which is the input test value and transmitted to the central server by utilizing the internet connection function of the mobile device .

FIG. 6 is an embodiment showing a GUI configuration of a mobile device application program according to the present invention. FIG. 7 shows an image and analysis result of an LFA strip reading result using a dengue virus sample according to the present invention. Referring to FIG. 7, it can be seen that the diagnosis of the LFA strip according to the present invention shows a significant result in comparison with the virus-free control (negative sample in FIG. 7) even when the virus sample is diluted 1.6-fold and 3.2-fold.

The in vitro diagnostic system having an attachment type optical system using a mobile device according to the present invention is composed of hardware and a program of a personal portable mobile device and a camera of a mobile device and is used for diagnosis or diagnosis of a point of care (POC) And the results of the GPS information, the location and the date and time embedded in the mobile device can be automatically transmitted to the central control data server through the communication function of the mobile device to effectively grasp the location and the frequency.

Also, it is suitable for self-diagnosis equipment for biological samples because the system equipment can be used semi-permanently only by replacing the membrane strip.

On the contrary, the foregoing detailed description is to be considered in all respects as illustrative and not restrictive. The scope of the present invention should be determined by rational interpretation of the appended claims, and all changes within the scope of equivalents of the present invention are included in the scope of the present invention.

Claims (10)

A strip sensor for detecting a biomaterial;
An attachment type optical system including a support for supporting the strip sensor, a fluorescent filter disposed on the support, a lens, and a light source; And
A mobile device capable of wireless communication with the attachment type optical system, comprising: a camera; and an application for discriminating the biomaterial from an image of the strip sensor taken by the camera;
And an attachment type optical system using the mobile device.
The optical system according to claim 1,
A support for supporting the strip sensor;
A fluorescent filter disposed on an upper portion of the strip sensor window;
A lens disposed on top of the fluorescence filter; And
A light source disposed on left and right sides of the fluorescent filter;
And an attachment system optical system using the mobile device.
The optical system according to claim 1,
Wherein the strip sensor comprises a black-out sheet on an upper surface of the strip sensor.
The method according to claim 1,
Wherein the wireless communication is a Bluetooth communication.
The mobile terminal of claim 1,
An application execution unit for executing the application;
A control unit for controlling the light source of the optical system and turning it on when the application is executed;
An image capturing and processing unit for capturing the strip sensor continuously or step by step using a camera provided in the mobile device while the application is executed;
An image analysis and determination unit for analyzing the photographed image to determine a biomarker concentration; And
A judgment information display section for displaying a judgment result of the image analysis and judgment section;
Further comprising: an attachment system optical system using a mobile device.
6. The method of claim 5,
Wherein the attachment type optical system further comprises a drive circuit unit in the light source.
The method according to claim 6,
In order to perform an in vitro diagnosis through an in vitro diagnostic system having an attachment type optical system using the mobile device,
Wherein the application of the mobile device controls the amount of light by applying power to the light source through the driving circuit portion of the attachment type optical system,
The image capturing and processing unit captures the strip sensor continuously or step by step using a camera provided in the mobile device in a state where the application is executed,
Wherein the image analysis and determination unit analyzes the photographed image to determine a biomarker.
The method according to claim 1,
Wherein the strip sensor is a strip for acute viral diagnosis or acute myocardial infarction diagnosis.
9. The method of claim 8,
Wherein the strip sensor is an LFA strip using a fluorescent material as a label.
The method according to claim 1,
Wherein the light source of the optical system is disposed on both sides or in four directions of the strip sensor window to uniformly illuminate the window of the strip sensor.

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