KR20190024786A - Composition for preventing, improving or treating metabolic disease containing mixture of perilla oil containing omega-3 fatty acid, tomato extract and paprika extract as effective component - Google Patents

Abstract

The present invention relates to a composition for preventing, alleviating, or treating metabolic diseases, comprising a mixture of perilla oil containing omega-3 fatty acids, tomato extracts, and paprika extracts as an active ingredient. More specifically, the mixture of perilla oil containing omega-3 fatty acids, tomato extracts, and paprika extracts had been shown to significantly reduce levels of liver triglycerides and serum cholesterols in test animals fed on a high-fat diet for a long period; suppress lipid accumulation in thoracic aorta and liver tissues; and effectively reduce blood triglyceride levels, total cholesterol levels, and LDL cholesterol levels that were elevated due to a high-cholesterol diet. Accordingly, the present invention may be beneficially used in the development of functional health foods or drugs for preventing, alleviating, or treating metabolic diseases such as non-alcoholic fatty liver, cardiovascular diseases, and the like.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for preventing, improving or treating a metabolic disease containing a mixture of omega-3 fatty acid-containing oil, a tomato extract and a paprika extract as an active ingredient. 3 fatty acid, tomato extract and paprika extract as effective component}

The present invention relates to a composition for preventing, ameliorating or treating a metabolic disease containing an omega-3 fatty acid containing perilla oil, a tomato extract, and a paprika extract as an active ingredient.

Along with the development of the industry, changes in the diet of instant food and flesh-eating have become the main causes of the increase in the obese population. Obesity is caused by changes in hormones caused by excessive fat intake and by adipocyte differentiation due to unbalanced energy metabolism. Adipogenesis in the body is differentiated from adipocyte (preadipocyte) As a process of accumulation, mature adipocytes differentiate from immature adipocytes such as fibroblasts and ultimately form lipid droplets within the cells. As such, the accumulation of fat and excess energy during adipocyte differentiation not only induces obesity but also causes various lipid-related metabolic diseases such as diabetes, hyperlipidemia, fatty liver, arteriosclerosis, hypertension, cardiovascular disease, Metabolic syndrome, and the like.

Non-alcoholic fatty liver disease (NAFLD) is defined as the accumulation of fatty acids in the form of triglycerides in the parenchymal cells of the liver in excess of 5%, not by liver damage caused by alcohol. Pathologically, it is classified as simple steatosis and inflammatory fatty liver disease (steatohepatitis). It can be converted to severe liver disease such as hepatitis, liver fibrosis and cirrhosis when left for a long time. In Korea, the frequency of nonalcoholic liver disease is increasing due to changes in lifestyle. Metformin, thiazolidinedione, statin drugs, and antioxidants have been tried for treatment. However, no medicines have been established as therapeutic agents until now, and hepatic enzyme levels There is no report on the partial improvement of liver biopsy, but there are few drugs recognized as comprehensive treatment including improvement of liver biopsy.

Cardiovascular system supplies the oxygen, nutrients, and hormones necessary for the activity of each organ cell through the circulation of blood. It also helps the physiological functions of the human body to be maintained, such as removing carbon dioxide, lactic acid, etc. . Cardiovascular disease has been the leading cause of death in Korea since the 1970s and has recently become the leading cause of death. Background Art Cardiovascular diseases are a group of abnormalities in the cardiovascular system including central and peripheral vascular diseases such as heart diseases and aortas, and include diseases such as hypertension, hyperlipemia, atherosclerosis, myocardial infarction, heart failure, arrhythmia and angina .

Korean Patent Laid-Open Publication No. 2017-0049606 discloses a combination of omega-3 fatty acid and SGLT-2 inhibitor for the treatment of liver disease, and Korean Patent No. 1076185 discloses a fatty liver improvement Lt; RTI ID = 0.0 > pharmaceutically < / RTI > However, a composition for preventing, improving or treating a metabolic disease containing a mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention as an active ingredient has not yet been disclosed.

The present invention has been made in view of the above-mentioned needs. In the present invention, a mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract is fed to a model mouse of high fat diet, When the amount of omega-3 fatty acid containing perilla oil, tomato extract, and paprika extract of the present invention was compared to that of omega-3 fatty acid-containing perilla oil alone, the amount of the omega-3 fatty acid- , And that the blood triglyceride, total cholesterol and LDL + VLDL cholesterol contents increased due to high cholesterol diet were statistically decreased.

In order to solve the above problems, the present invention provides a health functional food composition for preventing or ameliorating a metabolic disease which contains, as an active ingredient, a mixture of ointment oil containing omega-3 fatty acid, tomato extract and paprika extract.

The present invention also provides a pharmaceutical composition for the prevention or treatment of metabolic diseases containing, as an active ingredient, a mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract.

The mixture of the omega-3 fatty acid containing oil, the tomato extract and the paprika extract of the present invention was significantly reduced in the animal body fed with high fat diet for a long time and the triglyceride and serum cholesterol contents were significantly decreased, And is effective in reducing blood triglyceride, total cholesterol, and LDL cholesterol content, which is increased by high cholesterol diet, and thus is useful as a health functional food for preventing, improving or treating metabolic diseases such as nonalcoholic fatty liver disease and cardiovascular disease And can be usefully used for drug development.

FIG. 1 shows the effect of a mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention on fat accumulation in liver tissue of a model mouse induced by high fat diet. Liver tissues were stained with H & E (Hematoxylin-Eosin) according to a conventional method to examine the histological changes of liver cells due to fat accumulation. HFD Control diet with high fat diets (45% HFD; high fat diet); HFD + Red oil 200 was an experimental group in which a mixture (red oil 200 mg / kg / day) of a mixture of omega-3 fatty acid containing oil of the present invention, tomato extract and paprika extract of the present invention was added to high fat diets (45% HFD) HFD + Red oil 1000 is an experimental group in which a mixture of high fat diets (45% HFD) with omega-3 fatty acid containing oil of the present invention, tomato extract and paprika extract (Red oil 1000 mg / kg / day) was fed.
FIG. 2 shows the results of examining the effect of the mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention on total cholesterol concentration in serum in a model mouse induced by high fat diet.
FIG. 3 shows the results of examining the effect of the mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention on the concentration of hepatic triglyceride in a model mouse induced by high fat diet.
FIG. 4 shows the results of examining the effect of a mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention on a fat accumulation in the thoracic aorta in a model mouse induced by high fat diet. To examine the histological changes of the thoracic aorta due to fat accumulation, the thoracic aorta was stained with Oil Red O according to a conventional method.
FIG. 5 shows the results of examining the effect of the mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention on the blood triglyceride content in model mice induced by high cholesterol diet. ND is a normal diet group; HCD was high cholesterol diet group (35% fat, 1.25% cholesterol, 0.5% cholic acid); Corn + HCD is a high cholesterol and corn oil diet; perilla + HCD is high cholesterol and omega-3 perilla oil; And Tp perilla + HCD represent a mixture of high cholesterol and omega-3 fatty acid containing oil of the present invention, tomato extract and paprika extract. * Was higher in the high cholesterol diet group (HCD) than in the normal diet group (ND); High cholesterol and corn oil diet group (Corn + HCD); And the effect of increasing the triglyceride content of perilla + HCD in the high cholesterol and omega-3 perilla oil group is statistically significant, meaning that the p value is less than 0.05. (Tp perilla + HCD), which is a mixture of high cholesterol and omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention, compared with the high cholesterol diet group (HCD) , Meaning that the p value is less than 0.05.
FIG. 6 shows the results of examining the effect of the mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention on the total cholesterol content in blood in a model mouse induced by high cholesterol diet. * Was higher in the high cholesterol diet group (HCD) than in the normal diet group (ND); High cholesterol and corn oil diet group (Corn + HCD); High cholesterol and omega-3 perilla oil (perilla + HCD); And omega-3 fatty acid containing olive oil, tomato extract and paprika extract (Tp perilla + HCD) were statistically significant, which means that the p value is less than 0.05. (Tp perilla + HCD), which is a mixture of high cholesterol and the omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention, compared with the high cholesterol diet group (HCD) , Meaning that the p value is less than 0.05. $ Of total cholesterol (Tp perilla + HCD) of the mixture diets (Tp perilla + HCD) containing the omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention as compared to the group of perilla + HCD containing high cholesterol and omega- The effect of reducing the content is statistically significant, meaning that the p-value is less than 0.05.
FIG. 7 shows the results of examining the effect of the mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention on the blood LDL + VLDL cholesterol content in a model mouse induced by high cholesterol diet. * Was higher in the high cholesterol diet group (HCD) than in the normal diet group (ND); High cholesterol and corn oil diet group (Corn + HCD); High cholesterol and omega-3 perilla oil (perilla + HCD); And omega-3 fatty acid perilla oil, tomato extract and paprika extract, showed a statistically significant effect of increasing the LDL + VLDL cholesterol level in the blood of the group (Tp perilla + HCD), which means that the p value is less than 0.05. Reduction of LDL + VLDL Cholesterol Levels in the Blood Group (Tp perilla + HCD) Mixed with High Cholesterol and Omega-3 Fatty Acids, Oatmeal Extract and Paprika Extract of the Present Invention Compared to High Cholesterol Diet Group (HCD) Is statistically significant, meaning that the p value is less than 0.05. $ Is the ratio of serum LDL + HCD of the mixture of the omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention (Tp perilla + HCD) to that of the perilla + HCD containing high cholesterol and omega- The VLDL cholesterol content reduction effect is statistically significant, meaning that the p-value is less than 0.05.

In order to achieve the object of the present invention, the present invention provides a health functional food composition for preventing or ameliorating a metabolic disease, which comprises a mixture of omega-3 fatty acid containing olive oil, tomato extract and paprika extract as an active ingredient.

In the health functional food composition for preventing or ameliorating the metabolic diseases according to the present invention, the tomato extract and the paprika extract may be prepared by dissolving tomato and paprika powder at a temperature of 40 to 60 DEG C and a pressure of 30 to 50 bar for 2 to 4 hours, Carbon dioxide, and may be extracted with supercritical carbon dioxide at a temperature of 50 ° C and a pressure of 40 bar for 3 hours, but the present invention is not limited thereto.

The mixture may contain 93 to 97 parts by weight of omega-3 fatty acid-containing oil, 1.5 to 3.5 parts by weight of tomato extract, and 1.5 to 3.5 parts by weight of paprika extract, based on 100 parts by weight of the whole, But are not limited to, an omega-3 fatty acid-containing oil, 2.5 parts by weight of tomato extract, and 2.5 parts by weight of paprika extract.

In addition, the metabolic diseases may be obesity, non-alcoholic fatty liver disease, or cardiovascular disease, but are not limited thereto.

In addition, the cardiovascular diseases may be selected from the group consisting of atherosclerosis, hypertension, angina pectoris, hyperlipidemia, myocardial infarction, stroke and heart failure, but the present invention is not limited thereto.

In addition, the composition may be formulated into any one form of powder, granule, ring, tablet, capsule, candy, syrup and beverage, but is not limited thereto.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is, or may be used together with other food or food ingredients, and suitably used according to a conventional method. The amount of the active ingredient can be appropriately used depending on the purpose of use thereof. Generally, the health functional food composition of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight based on the raw material, when the food or beverage is produced. However, in case of long-term ingestion for health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

There is no particular limitation on the kind of the health functional food. Examples of the foods to which the health functional food composition can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen and other noodles, gums, ice cream, soups, Drinks, alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

In addition, the health functional food composition of the present invention can be produced as a food, particularly a functional food. The functional food of the present invention includes components that are ordinarily added in food production, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, in the case of a drink, a natural carbohydrate or a flavoring agent may be included as an additional ingredient in addition to the active ingredient. The natural carbohydrate may be selected from the group consisting of monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose etc.), oligosaccharides, polysaccharides (e.g., dextrin, cyclodextrin, , Xylitol, sorbitol, erythritol, etc.). The flavoring agent may be a natural flavoring agent (e.g., tau Martin, stevia extract, etc.) and a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).

In addition to the above health functional food composition, it is also possible to use various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, A carbonating agent used in beverages, and the like.

The present invention also provides a pharmaceutical composition for the prevention or treatment of metabolic diseases containing, as an active ingredient, a mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract.

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients or diluents conventionally used in the manufacture of pharmaceutical compositions.

The pharmaceutical dosage forms of the compositions according to the invention may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable set.

The pharmaceutical composition according to the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterilized injection solutions according to a conventional method . Examples of carriers, excipients and diluents that can be contained in the pharmaceutical composition including the mixture of omega-3 fatty acid containing oil, tomato extract and paprika extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol , Maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium Stearates, mineral oils, and the like. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like. These solid preparations may contain at least one excipient, for example, in a mixture of omega-3 fatty acid containing olive oil, tomato extract and paprika extract For example, it is prepared by mixing starch, calcium carbonate, sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include withexol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the drug form, the administration route and the period, but can be appropriately selected by those skilled in the art.

The pharmaceutical composition for preventing or treating metabolic diseases containing the mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention as an active ingredient can be administered to mammals such as rats, mice, livestock, ≪ / RTI > All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Manufacturing example  1. Preparation of a blended mixture of omega-3 fatty acid-containing oil, tomato extract and paprika extract

Tomatoes and paprika were lyophilized and lyophilized in good quality and unaltered, and then treated with supercritical carbon dioxide (SC-CO2) and extracted at a pressure of 40 MPa and a temperature of 50 ° C for 3 hours. At this time, the flow rate of carbon dioxide was 20 kg CO ₂ / h. The extract was kept in a dark room at -20 ° C. To suppress lipid oxidation during perilla milking, it was compressed at low temperature at room temperature and then stored in a dark room at -20 ° C. Respectively. The mixture of the present invention was prepared by mixing 95 parts by weight of omega-3 fatty acid containing perilla oil, 2.5 parts by weight of tomato extract and 2.5 parts by weight of paprika extract, based on 100 parts by weight of the whole.

Example  1. Design and experimental formula of high fat dietary animal

Male mice (6 weeks old, C57BL / 6) were adapted to the laboratory environment with general solid feed for one week and were randomly assigned to two groups (n = 8 per group) according to the nodule method and were bred for 13 weeks. Control diet with high fat diets (45% HFD; high fat diet); (Red oil 200 mg / kg / day and Red oil 1000 mg / kg / day) mixed with the omega-3 fatty acid containing perilla oil of the present invention, tomato extract and paprika extract were added to high fat diets (45% HFD) Experimental group. The temperature was 23 ± 2 ℃, the humidity was 50 ± 5% and the illumination period was 12 weeks.

Example  2. Weight and weight of high-fat diets Dietary intake  Measure

During the experimental period, the body weight gain was measured once every two days for the experimental animals, and they were bred for 13 weeks. Dietary intake and body weight were measured and recorded at regular intervals. Body weight and food intake were measured 2 hours after the feeding tube was removed to prevent a sudden change in body weight due to feed intake.

Total weight gain = final weight - initial weight

Daily average weight gain = total weight gain / days

Average Daily Dietary Intake: Total Intake / Days

Food efficiency ratio (FER) = [total weight gain / total intake] X 100

Dietary intake, weight gain and dietary efficiency measurements group Dietary intake (g / day) Weight gain (g / day) Dietary efficiency (FER,%) HFD 2.51 19.60 1.6 7.82 HFD + Red oil 200 1.94 14.10 ± 1.1 7.29 HFD + Red oil 1000 1.84 13.40 ± 0.8 7.28

HFD Control diet with high fat diets (45% HFD; high fat diet); HFD + Red oil 200 was an experimental group in which a mixture (red oil 200 mg / kg / day) of a mixture of omega-3 fatty acid containing oil of the present invention, tomato extract and paprika extract of the present invention was added to high fat diets (45% HFD) HFD + Red oil 1000 is an experimental group in which a mixture of high fat diets (45% HFD) with omega-3 fatty acid containing oil of the present invention, tomato extract and paprika extract (Red oil 1000 mg / kg / day) was fed.

Example  3. Tissue analysis of nonalcoholic fatty liver in high fat diabetic mice

In order to perform histopathological observation on liver tissues, tissues were excised and fixed in 10% formalin solution for 24 hours, dehydrated with graded alcohol, embedded in paraffin to make blocks, The tissue sections of 5 μm thickness were prepared with a microtome and stained with H & E (Hamatoxylin & eosin), washed with xylene, treated with a permount, and then subjected to CKX41 optical microscopy to detect specific lesions Respectively.

As a result, a mixture (45% HFD + Red oil 200) containing the omega-3 fatty acid containing perilla oil of the present invention, a tomato extract and a paprika extract of the present invention as compared with the group (45% HFD) And 45% HFD + Red oil 1000) showed a decrease in the number of lipid droplets showing white in liver, indicating that the degree of neutral fat deposition in liver was decreased.

Example  4. Analysis of serum cholesterol and triglyceride content in high fat diabetic mice

Blood samples were collected from each group of experimental animals on day 90 after onset of reperfusion or through the eyes of mice. After centrifugation, serum total cholesterol levels were measured by the colorimetric assay using the Chemilab T-CHO assay kit and the triglyceride colorimetric assay kit for liver triglyceride levels was measured. As a result, as shown in FIG. 2, the total cholesterol level in serum was as high as 159.5 ± 11.3 mg / dL in the group (45% HFD) fed with high fat diets, while the omega-3 fatty acid- , 139.9 ± 5.5 mg / dL and 139.9 ± 14.4 mg / dL, respectively, in the case of a mixture of a mixture of tomato extract and paprika extract (45% HFD + Red oil 200 and 45% HFD + Red oil 1000) And the blood lipid concentration was significantly reduced. In addition, the triglyceride content of the liver was high (203.9 ± 19.1 mg / g) in the group fed the high fat diet (45% HFD), while the omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention The results were statistically significant, with 172.2 ± 29.2 mg / dL and 177.4 ± 9.9 mg / dL, respectively, when the mixture (45% HFD + Red oil 200 and 45% HFD + Red oil 1000) were fed.

Example  5. Analysis of chest aortic fat accumulation in high fat diet mouse

The thoracic aortic tissue was fixed in 10% formalin solution for 4 hours and frozen. The frozen tissue was fixed in 10% formalin solution for 10 minutes, and then the section was washed with distilled water and air -Dried). Subsequently, the sections were incubated in a 0.5% oil red O working solution for 5 minutes, washed with distilled water, and counterstained for 1 minute with Meyer 'hematoxylin. The stained sections were loaded with glycerol gelatin and the presence of specific lesions was observed using a CKX41 optical microscope. As a result, as shown in FIG. 4, in the case of the group (45% HFD) in which the high fat diets were fed, the fat accumulation was clearly visible in the blood vessels, while the omega-3 fatty acid containing perilla oil, (45% HFD + Red oil 200 and 45% HFD + Red oil 1000) mixed with paprika extract were found to inhibit lipid deposition.

Example  6. Design and experimental formula of high cholesterol diet

Male mice (7 weeks old, C57BL / 6) were adapted to the laboratory environment for one week with general solid feeds and were randomly assigned to 5 groups (n = 8 per group) (ND); High cholesterol diet (35% fat, 1.25% cholesterol, 0.5% cholic acid, high cholesterol diet); High cholesterol and corn oil diet group (Corn + HCD); High cholesterol and omega-3 perilla oil (perilla + HCD); And high cholesterol and the omega-3 fatty acid containing oil of the present invention, tomato extract and paprika extract (Tp perilla + HCD). The temperature was 23 ± 2 ℃, the humidity was 50 ± 5%, the illumination period was 95 days, and the food and drinking water were self - fed.

Example  7. Measurement of blood triglyceride and cholesterol content in hypercholesterolemic mice

(1) Blood triglyceride content

Serum triglyceride content was measured using an assay kit. Specifically, 10 μl of serum and 150 μl of enzyme solution were put into a test tube and mixed, followed by reaction at room temperature for 15 minutes. After the reaction was completed, the absorbance was measured at a wavelength of 540 nm using the used reagent as a blank. As a result, as shown in FIG. 5, a mixture of the omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention (Tp perilla + HCD), which was increased due to high cholesterol diet (HCD) . The results of this study are as follows. On the other hand, in the case of high cholesterol and corn oil diet (Corn + HCD) and high cholesterol and omega-3 perilla oil diet (perilla + HCD) . That is, the mixture of the omega-3 fatty acid containing perilla oil, the tomato extract and the paprika extract of the present invention was more effective in reducing the blood triglyceride content, which was increased due to the high cholesterol diet, than the omega-3 fatty acid containing perilla oil alone.

(2) Blood cholesterol content

Total cholesterol and LDL (low density lipoprotein) - cholesterol are major risk factors for coronary artery disease. In general, total cholesterol concentration is closely related to LDL-cholesterol concentration, and LDL-cholesterol accounts for about 80% of total cholesterol. Very low density lipoprotein (VLDL) is a precursor of LDL and mainly contains triglycerides. Therefore, serum total cholesterol and LDL + VLDL cholesterol contents were measured using an assay kit. Specifically, 50 혈 of serum was mixed with 50 콜 of cholesterol reaction reagent, followed by reaction at 37 캜 for 60 minutes, and the absorbance was measured at 570 nm using distilled water as a blank. As a result of measurement of the total cholesterol content, as shown in FIG. 6, the total cholesterol content in blood, which was increased due to high cholesterol diet (HCD), was higher than that of the mixture of omega-3 fatty acid containing perilla oil, tomato extract and paprika extract Tp perilla + HCD) significantly reduced the total cholesterol level in the blood compared with the high-cholesterol and omega-3 perilla oil group (perilla + HCD) Respectively. In addition, in the case of high cholesterol and corn oil diet (Corn + HCD) and high cholesterol and omega-3 perilla oil diet (perilla + HCD) used as the comparative control group, statistically significant difference was observed between the high cholesterol diet group . That is, the mixture of the omega-3 fatty acid containing perilla oil, the tomato extract and the paprika extract of the present invention is more excellent in reducing the total cholesterol content in the blood due to the high cholesterol diet than the omega-3 fatty acid containing perilla oil alone Respectively.

Also, as shown in FIG. 7, when the blood LDL + VLDL cholesterol content increased due to the high cholesterol diet (HCD) was higher than that of the mixture of the omega-3 fatty acid containing perilla oil, tomato extract and paprika extract of the present invention (Tp perilla + HCD) significantly decreased, and the LDL + VLDL cholesterol content was significantly decreased compared to the perilla + HCD group, especially the high cholesterol and omega - 3 perilla oil group. On the other hand, in the case of high cholesterol and corn oil diet (Corn + HCD) and high cholesterol and omega-3 perilla oil diet (perilla + HCD) . That is, the mixture of the omega-3 fatty acid containing perilla oil, the tomato extract and the paprika extract of the present invention has an effect of lowering the blood LDL + VLDL cholesterol level, which is increased due to the high cholesterol diet, than the omega-3 fatty acid containing perilla oil alone Excellent.

Claims (9)

A composition comprising a mixture of omega-3 fatty acid-containing oil, a tomato extract, and a paprika extract as an active ingredient. [Claim 2] The method according to claim 1, wherein the tomato extract and the paprika extract are respectively extracted with supercritical carbon dioxide at a temperature of 40 to 60 DEG C and a pressure of 30 to 50 bar for 2 to 4 hours, A health functional food composition for preventing or ameliorating disease. The method according to claim 1, wherein the mixture comprises 93 to 97 parts by weight of omega-3 fatty acid containing perilla oil, 1.5 to 3.5 parts by weight of tomato extract and 1.5 to 3.5 parts by weight of paprika extract per 100 parts by weight of the total A health functional food composition for preventing or ameliorating disease. The health functional food composition according to claim 1, wherein the metabolic disease is obesity, non-alcoholic fatty liver disease or cardiovascular disease. The health functional food composition according to claim 4, wherein the cardiovascular disease is selected from the group consisting of arteriosclerosis, hypertension, angina pectoris, hyperlipidemia, myocardial infarction, stroke and heart failure. The health functional food composition according to claim 1, wherein the composition is prepared from any one of powder, granule, ring, tablet, capsule, candy, syrup and beverage. A pharmaceutical composition for preventing or treating a metabolic disease containing an omega-3 fatty acid-containing oil, a tomato extract, and a mixture of paprika extract as an active ingredient. [Claim 7] The pharmaceutical composition according to claim 7, further comprising a pharmaceutically acceptable carrier, excipient or diluent in addition to the active ingredient. The pharmaceutical composition according to claim 7, wherein the metabolic disease is obesity, non-alcoholic fatty liver disease or cardiovascular disease.

Images