KR20190018122A - A method of preparing seasoned spawn of alaska pollack using laminaria extract and seasoned spawn of alaska pollack prepared therefrom - Google Patents
A method of preparing seasoned spawn of alaska pollack using laminaria extract and seasoned spawn of alaska pollack prepared therefrom Download PDFInfo
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- KR20190018122A KR20190018122A KR1020170102676A KR20170102676A KR20190018122A KR 20190018122 A KR20190018122 A KR 20190018122A KR 1020170102676 A KR1020170102676 A KR 1020170102676A KR 20170102676 A KR20170102676 A KR 20170102676A KR 20190018122 A KR20190018122 A KR 20190018122A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/30—Fish eggs, e.g. caviar; Fish-egg substitutes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/60—Edible seaweed
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/202—Algae extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/24—Heat, thermal treatment
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- Life Sciences & Earth Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Edible Seaweed (AREA)
Abstract
Description
본 발명은 다시마 추출물을 이용하는 조미 명란 제조방법 및 상기 제조방법에 의해 제조된 조미 명란에 관한 것이다.The present invention relates to a method for producing seasoning starch using seaweed extract and a seasoning starter prepared by the method.
명란은 명태(Walleye Pollock)의 알을 의미하는 것으로 기능성이 탁월하면서도 맛이 좋아 전통적으로 명란 젓갈의 형태로 식재료로 이용하고 있다. 명란은 비타민 A, 비타민 B군, 비타민 E 및 무기질 함량이 높고, 단백질의 함량이 풍부하며 특히 불포화 지방산인 리놀렌산(linolenic acid)의 함량이 높다고 알려져 있다.Myeonbang means the egg of Walleye Pollock. It is excellent in functionality but it is traditionally used as a foodstuff in the form of salted salted fish. It is known that lentil is rich in vitamin A, vitamin B group, vitamin E and minerals, rich in protein, and especially high in linolenic acid, an unsaturated fatty acid.
명란은 자체가 가지고 있는 비린풍취가 있어서 식재료로 활용하는데 어려움이 있고, 최근 명태를 포함한 대구과에 속하는 어류 즉, 명태류의 어획량의 감소와 함께 가격이 급등하고 있기도 하다.It has difficulties to use it as a foodstuff because it has its own pungent flavor. Recently, the price of fish, which belong to the daegu section including pollack, has been rapidly increasing along with the decrease in the catch of pollack.
상기 명란을 활용한 식품 제조에 있어서, 명란의 비린풍취를 제어하는 것은 중요한 요소이며, 과거에는 주로 비린 풍취도 제거되고, 장시간 보관도 가능한 젖갈 형태로 제조되어 유통되어 왔다.In the production of food utilizing the above-mentioned method, controlling the pungency of the pungency is an important factor. In the past, the pungency has been produced and distributed in the form of a poultry which can be stored for a long time.
명란젓은 염장품에 가까우나 숙성공정이 반드시 필요한 점에서 젓갈로 분류되며, 냉동 및 냉장조건으로 유통되고 있다.The cod roe is similar to salted roe, but it is categorized into salted roe because it is necessary to aging process.
그러나, 생활환경 변화, 구체적으로 심각한 환경오염, 업무 스트레스, 고열량과 화학조미료 함량이 높은 가공식품 섭취와 같은 식생활 패턴의 변화로 인하여 다양한 대사성 질환 및 순환계 질환 발병의 증가라는 심각한 문제점에 직면하고 있는 현대사회에서는 염장 식품에 대한 거부감이 있으며, 특히나 최근 웰빙을 강조하는 생활 습관과 건강에 대한 관심의 증대로 인하여, 소비자는 고염 식품을 기피하고 저염 식품에 대한 선호도가 높아지고 있다.However, there are serious problems such as increasing the incidence of diverse metabolic diseases and circulatory diseases due to changes in dietary patterns such as changes in living environment, specifically severe environmental pollution, work stress, high calorie content, and high processed food intake, In society, there is a feeling of refusal to eat salted foods. Especially, due to lifestyle emphasizing wellbeing and increasing interest in health, consumers are avoiding high salt foods and preference for low salt foods is increasing.
따라서, 기존에 염장 방법이 아닌 소금 사용의 억제로 인해 염도가 낮으면서도 유의하게 명란의 비린 맛을 제어할 수 있는 연구의 필요성이 증대되고 있다.Therefore, there is an increasing need for studies that can control the salty taste of salmon, which is low in saltiness due to the suppression of the use of salt rather than salting method.
본 발명은 기존 명란 비린 맛 제거를 위해 염장을 진행하는 명란젖과 달리 소금 사용을 최소화 하면서도 유효하게 명란의 비린 맛을 제거하여 기호도 개선 및 저염 식품 제조라는 목적을 달성하는 조미 명란 제조방법을 제공하는 것을 목적으로 한다.The present invention provides a method of manufacturing a seasoning hot pepper which achieves the object of improving the palatability and the production of a low-salt food by removing the palatability of the palatability, while minimizing the use of the salt, unlike the palatable palatable milk, .
또한, 본 발명의 다른 목적은 상기 제조방법에 의하여 제조된 조미 명란을 제공하는 것이다.Another object of the present invention is to provide a seasoning coloring prepared by the above-mentioned production method.
본 발명은 상기 목적을 달성하기 위하여, 기존 소금을 이용하여 염장하는 방식으로 명란의 비린맛을 제어하거나, 보관기간 또는 유통기간을 연장시키는 기존 연구와 달리, 식물 추출물을 이용하여 조미함으로써 명란의 비린 맛을 제거하는 개선된 조미 명란 제조방법을 제공한다.In order to achieve the above object, unlike existing researches which control salty flavor of Salmonella or extend shelf life or shelf life by salting with existing salt, The present invention provides an improved seasoning process for removing flavor.
구체적으로, 상기 조미 명란 제조방법은 다시마 추출물을 제조하는 단계; 명란을 소금물에 세척하는 명란 준비 단계; 및 상기 명란을 다시마 추출물이 포함된 침지액에 침지하고 숙성시키는 조미 명란 제조단계를 포함하는 것일 수 있다.Specifically, the method for preparing the seasoned sea bream includes: preparing seaweed extract; A washing step to wash the nameplate in brine; And a seasoning preparation step of immersing and stirring the albumen in an immersion liquid containing seaweed extract.
상기 다시마 추출물을 제조하는 단계는 다시마 분말에 70% 에탄올 수용액을 첨가하고 추출하여 제조된 것일 수 있다.The step of preparing the kelp extract may be one prepared by adding 70% ethanol aqueous solution to the kelp powder.
상기 다시마 추출물은 다시마 분말 100 중량부에 70% 에탄올 수용액 1,500 중량부를 첨가하고 60℃ 내지 80℃ 조건에서 10분 내지 120분 동안 추출한 후, 여과, 감압농축 및 -20℃에서 동결건조과정을 수행하여 제조된 것일 수 있다.The seaweed extract was prepared by adding 1,500 parts by weight of 70% ethanol aqueous solution to 100 parts by weight of seaweed powder, extracting it at 60 ° C to 80 ° C for 10 minutes to 120 minutes, filtering, concentrating under reduced pressure and freeze- It may be manufactured.
상기 다시마 추출물이 포함된 침지액은 물에 상기 다시마 추출물을 5%(w/w) 농도로 첨가하여 제조된 것일 수 있다.The immersion liquid containing the kelp extract may be prepared by adding the kelp extract to water at a concentration of 5% (w / w).
또한, 본 발명의 조미 명란은 상기 조미 명란의 제조방법으로 제조된 것일 수 있다.
In addition, the seasoning name of the present invention may be one prepared by the method of manufacturing the seasoning name list.
본 발명의 발명자는 조미 명란의 제조에 있어서, 명란의 비린 맛을 제거하여 기호도를 개선함에 있어서, 기존 염장방법과 달리 소금의 사용량을 최소화함으로써 기존 명란젖 등이 가지는 문제점을 해결함으로써 조미 명란 품질 개선을 위해 연구를 진행하던 중, 기존 음식의 조미과정에 사용되는 재료인 다시마를 사용하여 조미액을 만들고 이에 침지시키는 경우, 명란의 비린 맛을 제어할 수 있음을 확인하였고, 추가로 연구한 결과 70% 에탄올 수용액 다시마 추출물을 5%(w/w) 첨가한 명란 조미액에 침지시키는 것이 비린 맛 제거 등 기호도 향상 및 항산화 효과 증진 등 기능성 개선 측면에서도 가장 효과적임을 확인하여, 이를 기초로 본 발명을 완성하였다.
The inventor of the present invention has found that, in the preparation of seasoning lemongrass, in improving the preference degree of the lemongrass, the use of salt is minimized unlike the conventional salting method, thereby solving the problems of existing lemongrass , It was confirmed that when the seasoning liquid was prepared and dipped in seaweed, it was possible to control the pungent flavor of the pungrang, and further study showed that 70% The present inventors completed the present invention on the basis of this finding by confirming that dipping in sea tangle seasoning added with 5% (w / w) of an aqueous solution of an aqueous solution of ethanol tincture is most effective in improving the functionality such as improving the palatability and improving the antioxidative effect.
이하, 본 발명을 보다 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명은 다시마를 이용한 조미 명란 제조방법에 관한 것이다.The present invention relates to a method for preparing seasoned sea bream using sea tangle.
상기 다시마를 이용한 조미 명란의 제조방법은 다시마 추출물을 제조하는 단계; 명란을 소금물에 세척하는 명란 준비 단계; 및 상기 명란을 다시마 추출물이 포함된 침지액에 침지하고 숙성시키는 조미 명란 제조단계를 포함하는 것일 수 있다.The method for preparing seasoned sea tangle using the kelp comprises the steps of: preparing seaweed extract; A washing step to wash the nameplate in brine; And a seasoning preparation step of immersing and stirring the albumen in an immersion liquid containing seaweed extract.
구체적으로 상기 다시마를 이용한 조미 명란의 제조방법은 다시마 분말 100 중량부에 70% 에탄올 수용액 1,500 중량부를 첨가하고, 70℃ 조건에서 30분 동안 추출한 후, 여과, 감압농축 및 -20℃에서 동결건조과정을 수행하여 다시마 추출물을 제조하는 다시마 추출물 제조단계; 명란을 소금물에 염지한 후, 유수에서 세척하여 염지 명란을 준비하는 명란 준비 단계; 및 물에 상기 다시마 추출물을 5%(w/w) 농도로 첨가하여 제조한 다시마 추출물이 포함된 침지액 100 중량부에 상기 염지 명란 200 중량부를 침지시키고 5℃에서 48시간 동안 숙성시키는 조미 명란 제조단계를 포함하는 것일 수 있다.Specifically, 1,500 parts by weight of a 70% ethanol aqueous solution was added to 100 parts by weight of tallow powder, followed by extraction at 70 ° C for 30 minutes, followed by filtration, concentration under reduced pressure and lyophilization at -20 ° C To produce seaweed extract; A brightening preparation step in which bright coloring is salted with brine, and then washing is carried out in running water to prepare a bright coloring clear coloring; And 100 parts by weight of an immersion liquid containing sea tangle extract prepared by adding the above sea tangle extract to water at a concentration of 5% (w / w), 200 parts by weight of the above- Step < / RTI >
상기 다시마 추출물 제조단계는 물, 유기용매 및 이들의 혼합물을 이용하여 추출과정을 수행할 수 있으며, 기호도의 개선 및 기능성 향상이란 측면에서 상기 다시마 추출물은 바람직하게는 에탄올 수용액, 더욱 바람직하게는 70% 에탄올 수용액을 이용하여 수행할 수 있다.The kelp extract may be extracted using water, an organic solvent, or a mixture thereof. The kelp extract is preferably an aqueous solution of ethanol, more preferably 70% or more, Ethanol aqueous solution.
구체적으로, 다시마 추출물 제조단계는 다시마 분말 100 중량부에 70% 에탄올 수용액 1,500 중량부를 첨가하고, 60℃ 내지 80℃ 조건에서 10분 내지 120분 동안 추출한 후, 여과, 감압농축 및 - 20℃에서 동결건조과정을 수행하여 다시마 추출물을 제조하는 방법으로 수행할 수 있다.Specifically, in the step of preparing kelp extract, 1,500 parts by weight of 70% ethanol aqueous solution was added to 100 parts by weight of tallow powder, and the mixture was extracted at 60 ° C to 80 ° C for 10 minutes to 120 minutes, followed by filtration, Followed by drying to produce seaweed extract.
더욱 구체적으로, 상기 다시마 추출물 제조단계는 다시마 분말 100 중량부에 70% 에탄올 수용액 1,500 중량부를 첨가하고, 70℃ 조건에서 30분 동안 추출한 후, 여과, 감압농축 및 -20℃에서 동결건조과정을 수행하여 다시마 추출물을 제조하는 방법으로 수행할 수 있다.More specifically, in preparing the kelp extract, 1,500 parts by weight of 70% ethanol aqueous solution was added to 100 parts by weight of tallow powder, and the mixture was extracted at 70 ° C for 30 minutes, followed by filtration, concentration under reduced pressure and freeze- To thereby produce a sea tangle extract.
상기 명란 준비 단계는 명란을 소금물에 세척하는 방법으로 수행할 수 있다.The above preparation step can be carried out by washing the nameplate with brine.
상기 명란 준비 단계는 조미명란을 제조하기 위한 명란을 준비하는 것으로, 난막이 터지지 않은 명란, 즉 난막 안에 존재하는 명란을 바람직하게 사용할 수 있으며, 상기 명란 중에서도 조미 단계 이전의 명란을 명란 원란 이라고도 한다. 상기 난막 안에 존재하는 명란을 소금물에 세척하는 방법으로 상기 명란을 준비할 수 있다. In the preparation step of preparing the name, it is preferable to use a name which is not in the albumen, that is, a name in the albumen. The above description can be prepared by washing the bright color in the egg shell with brine.
상기 명란이란 명태류의 알을 의미하는 것으로 기능성이 탁월하면서도 맛이 좋아 전통적으로 명란 젓갈의 형태로 식재료로 이용하고 있다. 상기 명란은 비타민 A, 비타민 B군, 비타민 E 및 무기질 함량이 높고, 단백질의 함량이 풍부하며 특히 불포화 지방산인 리놀렌산(lonolenic acid)의 함량이 높다고 알려져 있다. The above-mentioned name means eggs of roe-like rice, which is excellent in functionality but tastefully used as a foodstuff in the form of salted pickles. It is known that vitamin A, vitamin B group, vitamin E and mineral content are high, protein content is abundant, and especially the content of unsaturated fatty acid, lonolenic acid is high.
구체적으로, 상기 명란 준비 단계는 명란, 바람직하게는 명란 원란을 소금물에 염지한 후, 유수에서 세척하여 염지 명란을 준비하는 방법으로 수행할 수 있다.Specifically, in the preparation step of the bright color, the bright color, preferably the bright yellow color, is washed with brine, and then washed with running water to prepare a pale yellow color.
상기 명란 원란의 염지는 명란 원란에 부착된 불순물 제거 및 비린 맛의 제거란 측면에서 24시간 이상 수행하는 것이 바람직하고, 염분의 감소 및 짠 맛의 제어란 측면에서 96시간 이하 수행하는 것이 바람직하며, 이러한 차원에서 48시간 동안 수행하는 것이 가장 바람직하다.It is preferable that the poultice of the poultry poultry egg is performed for 24 hours or longer in terms of removing the impurities attached to the poultry poultry egg and removing the poultry tastes. It is preferable that the poultice poultice is performed for 96 hours or less in terms of reduction of salinity and control of salty taste. At this level, it is most desirable to perform for 48 hours.
상기 조미 명란 제조단계는 숙성 과정 및 물기 제거과정을 포함하는 것일 수 있다.The seasoning preparation step may include an aging process and a water removal process.
상기 숙성 과정은 상기 명란을 침지액에 침지시키고 숙성시키는 방법으로 수행할 수 있다. 구체적으로, 상기 숙성 과정은 상기 명란을 상기 명란 숙성액에 4℃ 내지 6℃ 조건에서 40 시간 내지 56 시간 동안 침지시켜 숙성시키는 방법으로 수행할 수 있다.The aging process can be carried out by immersing the field name in an immersion liquid and aging the same. Specifically, the aging may be performed by immersing the agar in the aged solution at 4 ° C to 6 ° C for 40 to 56 hours to age the agar.
구체적으로, 상기 조미 명란 제조단계는 물에 상기 다시마 추출물을 5%(w/w) 농도로 첨가하여 제조한 다시마 추출물이 포함된 침지액 100 중량부에 상기 염지 명란 200 중량부를 침지시키고 4℃ 내지 6℃에서 40시간 내지 56시간 동안 숙성시키는 방법으로 수행할 수 있다.Specifically, the step of preparing the seasoned sea bream is carried out by immersing 200 parts by weight of the sea bream name lemon in 100 parts by weight of an immersion liquid containing sea tangle extract prepared by adding the sea tangle extract to water at a concentration of 5% (w / w) And aging at 6 DEG C for 40 to 56 hours.
더욱 구체적으로, 상기 조미 명란 제조단계는 물에 상기 다시마 추출물을 5%(w/w) 농도로 첨가하여 제조한 다시마 추출물이 포함된 침지액 100 중량부에 상기 염지 명란 200 중량부를 침지시키고 5℃에서 48시간 동안 숙성시키는 방법으로 수행할 수 있다.More specifically, the step of preparing the seasoned sea bream is carried out by immersing 200 parts by weight of the sea bream name in 100 parts by weight of the immersion liquid containing the sea tangle extract prepared by adding the sea tangle extract at a concentration of 5% (w / w) For 48 hours.
상기 물기 제거 과정은 상기 숙성시킨 명란을 체에 받쳐 물기와 불순물을 제거하는 방법으로 수행할 수 있다.The water removal process may be carried out by removing the water and the impurities by supporting the aged mung bean on the sieve.
또한, 본 발명은 상기 제조방법에 의하여 제조된 조미 명란을 제공하는 것이다.In addition, the present invention provides a dried seasoning produced by the above-mentioned production method.
상기 본 발명의 조미 명란은 조미 명란 제조과정에 물에 70% 에탄올 수용액 다시마 추출물을 5%(w/w) 농도로 첨가하여 제조한 다시마 추출물이 포함된 침지액에 침지시킨 것일 수 있다. 상기 다시마 추출물 첨가에 의해 본 발명의 조미명란은 기능성이 부여되고, 비린 맛이 제거되었을 뿐만 아니라 최초 염장 후 유수에서 세척하여 명란에 포함된 염도를 최소화함으로써 최종 제품의 염도가 낮아서, 기호도가 개선되고, 염도도 제어되어, 기존 조미 명란에 비해 우수한 특징을 갖는다.The seasoning name of the present invention may be one prepared by adding 70% ethanol aqueous solution of sea tangle extract to water at a concentration of 5% (w / w) in water during the seasoning process, and immersing it in an immersion liquid containing sea tangle extract. By adding the above sea tangle extract, the seasoning of the present invention is imparted with functionality, and not only the malignant taste is removed, but also the salinity of the final product is lowered by washing it in the fresh water after the initial salting, , Salinity is also controlled, and it has superior characteristics compared to the existing seasoning.
본 발명의 제조방법은 지역적인 특성을 가진 식품으로 고부가가치 창출이 가능하여, 지역 경제에 도움이 되는 경제성이 높은 명란 특히, 기존 명란의 활용도 개선을 위해 비린 맛을 제어하는 조미 명란의 품질 개발에 관한 것으로, 기존 연구와 달리 최종 제품의 염도를 가능한 낮게 되도록 제어할 뿐만 아니라, 기호도를 개선하고 기능성도 향상시키는 것이어서, 최근 현대인들의 식생활습관과 웰빙 추세에 맞도록 소금 대신 다시마 추출물을 이용한 것으로, 기존 조미 명란에 비하여 기능성이 부가되었을 뿐만 아니라, 기호도도 개선되고, 염도도 낮추어, 명란과 관련된 여러 산업분야의 수익 향상에 이바지할 수 있으므로, 우수한 효과가 인정된다.The production method of the present invention is a food having local characteristics and can generate high added value, so that it can be economically beneficial to the local economy. In particular, to improve the utilization of the existing lantern, Unlike previous researches, it is aimed not only to control the salinity of the final product as low as possible, but also to improve the preference degree and improve the functionality. Recently, it has been used seaweed extract instead of salt to meet dietary habits and well- It is possible to improve not only the functionality but also the taste and the saltiness of the seasoning, thereby contributing to the improvement of profits in various industrial fields related to the sprouting.
도 1은 본 발명의 일 실시예에 따란, 침지액에 포함된 다시마 추출물 농도에 따른 최종 조미 명란에 포함된 세균 수를 측정한 결과를 나타낸 그래프이다.
도 2는 본 발명의 일 실시예에 따란, 침지액에 포함된 다시마 추출물 농도에 따른 관능 시험 결과를 나타낸 그래프이다.
도 3은 본 발명의 일 실시예에 따란, 침지액에 포함된 다시마 추출물 농도에 따른 항산화 효능을 확인한 결과를 나타낸 그래프이다.BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the results of measurement of the number of bacteria contained in the final seasonings according to the concentration of the sea tangle extract contained in the immersion liquid according to an embodiment of the present invention. FIG.
2 is a graph showing the results of sensory evaluation according to the concentration of sea tangle extract contained in the immersion liquid according to an embodiment of the present invention.
FIG. 3 is a graph showing the results of examining the antioxidant effect according to the concentration of the sea tangle extract contained in the immersion liquid according to one embodiment of the present invention.
이하, 본 발명을 상세히 설명하기 위하여 본 발명의 바람직한 실시예를 기재한다. 하기의 실시예는 본 발명을 예시하기 위한 것일 뿐 본 발명의 권리범위가 하기 실시예에 의해 어떤 식으로든 제한되는 것은 아니며, 당업계에서 통상적으로 수행되어지는 변형 등도 본 발명의 범위 내에 포함된다.
Hereinafter, preferred embodiments of the present invention will be described in detail to explain the present invention. The following examples are intended to illustrate the present invention, but the scope of the present invention is not limited in any way by the following examples, and variations and the like conventionally performed in the art are also included in the scope of the present invention.
제조예Manufacturing example : 다시마 추출물 및 조미 명란 제조: Preparation of sea tangle extract and seasoning
본 연구에 사용한 다시마 추출물은 수세, 탈염, 분쇄공정을 거쳐 적당한 크기로 자른 다시마 1 kg과 물, 30%(v/v) 주정(에탄올 수용액) 또는 70%(v/v) 주정(에탄올 수용액)을 15 kg을 혼합한 후, 70℃에서 30분간 추출을 수행한 후, 여과 및 감압농축하고, - 20℃에서 동결건조하여 다시마 추출물을 제조하였다. 상기 제조된 다시마 추출물은 실험 전까지 - 20℃ 냉동고에서 보관하였다.The kelp extract used in this study was prepared by adding 1 kg of kelp and water, 30% (v / v) alcohol (ethanol solution) or 70% (v / v) alcohol (aqueous ethanol solution) Were mixed, and the mixture was subjected to extraction at 70 ° C for 30 minutes, followed by filtration, concentration under reduced pressure, and lyophilization at -20 ° C to prepare seaweed extract. The kelp extract was stored at -20 ° C in a freezer until the experiment.
명란(삼양씨푸드㈜, 대한민국) 원란을 7%(w/v) 식염수를 이용하여 5℃에서 48시간 1차 염지한 후, 유수에서 세수하여 명란을 제조하였다.(Korean wan, Samyang Seed Food Co., Ltd., Korea) Wan Ran was first dipped in 7% (w / v) saline at 5 ° C for 48 hours and washed with distilled water.
이 후, 상기 각각의 용매를 사용하여 제조된 다시마 추출물을 물에 0, 0.5%(w/w), 1%(w/w), 2%(w/w), 5%(w/w) 및 10%(w/w)의 농도로 첨가하여 조미 침지액을 제조한 후, 상기 제조된 명란 100 g을 상기 조미 침지액 200 g에 첨가하고, 5℃에서 48시간 숙성하였다.Then, the sea tangle extract prepared using each of the above solvents was dissolved in water at 0, 0.5% (w / w), 1% (w / w), 2% (w / And 10% (w / w) to prepare a seasoned immersion liquid. To the seasoned immersion liquid (100 g) was added 100 g of the prepared sunflower, and the mixture was aged at 5 캜 for 48 hours.
상기 숙성한 조미 명란을 시료로 하여, 미생물 감염여부, 관능시험 및 항산화효능에 관한 실험을 수행하였다.
Experiments on the microbial infection, sensory test and antioxidant efficacy were carried out using the aged seasoned white rice as a sample.
실험예Experimental Example . 추출물 첨가량에 따른 평가. Evaluation according to the amount of extract
추출물 첨가량에 따른 개선 효과 평가를 위해, 미생물 감염 정도, 기호도 및 항산화 활성에 관한 실험을 수행하였다.In order to evaluate the improvement effect of the extracts, the degree of microbial infection, acceptability and antioxidant activity were investigated.
상기 미생물 감염 정도는 시료 내 세균 수(CFU/g)를 측정하는 방법으로 수행하였다. 또한, 기호도 평가는 관능평가는 10명 이상의 패널을 통하여 외관, 색, 냄새, 맛, 조직감 및 기호도를 중심으로 10점 척도에 의해 측정하였다. 또한, 항산화 활성은 구체적으로 DPPH radical 소거능(DPPH radical scavenging activity assay)을 확인하는 방법으로 수행하였다.The degree of microbial infection was measured by measuring the number of bacteria (CFU / g) in the sample. In addition, the sensory evaluation was performed on a 10-point scale based on appearance, color, smell, taste, texture and preference through 10 or more panels. In addition, the antioxidant activity was specifically confirmed by a method of confirming DPPH radical scavenging activity assay.
상기 미생물 감염 정도는 다음과 같이 식품공전의 미생물시험법, 일반세균수/대장균군 측정법에 의거하여 시험하였다. 구체적으로, 시료 10 g을 취하여 90 mL의 멸균 생리식염수를 가하여 균질화 한 후 시험용액 1 mL와 각 10배 단계 희석액 1 mL를 세균수 건조필름배지에 3매씩 접종한 후 35 내지 37℃에서 24 내지 48시간 배양한 후 생성된 붉은 집락수를 계산하고 그 평균집락수에 희석배수를 곱하여 일반세균수로 하였으며, 대장균군은 대장균군용 건조필름배지에 1ml을 3매씩 접종한 후 34 내지 36℃에서 24 내지 48시간 배양한 후 생성된 붉은 집락 중 주위에 기포를 형성하고 있는 집락수를 계산하고 그 평균집락수에 희석배수를 곱하여 대장균수를 산출하여, 도 1에 나타내었다.The degree of the microbial infection was tested in accordance with the following methods of food microorganism test, general bacterial count / coliform group assay as follows. Specifically, 10 g of the sample is taken and 90 mL of sterilized physiological saline is added to homogenize. Then, 1 mL of the test solution and 1 mL of each 10-fold dilution are inoculated into the bacterial water dry film medium, After 48 hours of culture, the number of red colonies was counted, and the average number of colonies was multiplied by a dilution factor to obtain a general bacterial count. The coliform group was inoculated with 3 ml of each 1 ml into a dry film medium for E. coli, The number of colonies forming air bubbles around the red colonies after culturing for 48 hours was calculated, and the average number of colonies was multiplied by the dilution factor to calculate the number of colon bacilli. The results are shown in FIG.
상기 도 1에 나타낸 바와 같이, 2일 간의 숙성기간 동안 세균은 1.8 x 102 내지 1.3 x 103 CFU/g의 범위를 나타내었으며, 다시마추출물 무처리구(1.3 x 103 CFU/g)와 비교하여 첨가구의 농도가 증가할수록 다소 감소하는 경향을 나타내었다. 이러한 결과는 상기 침지액에 포함된 다시마 추출물에 의한 것이라고 예상되었다.As shown in FIG. 1, the bacteria showed a range of 1.8 x 10 2 to 1.3 x 10 3 CFU / g during 2 days of aging, and compared with the untreated sea tangle extract (1.3 x 10 3 CFU / g) And decreased as the concentration of sphere increased. These results were expected to be due to the seaweed extract contained in the immersion liquid.
또한, 상기 관능평가는 10명이상의 패널을 통하여 향, 맛, 조직감 및 기호도를 중심으로 9점 척도에 의해 측정하였다. 상기 측정결과를 도 2에 나타내었다.In addition, the sensory evaluation was performed by a 9-point scale based on flavor, taste, texture, and preference through 10 or more panels. The measurement results are shown in Fig.
상기 도 2에 나타낸 바와 같이, 관능시험에서 다시마 추출물이 5% 농도로 첨가될 때까지는 다시마 추출물을 첨가하였을 때 대조구와 비교하여 냄새, 맛 및 전체적인 기호도가 증가되는 것으로 나타났으나, 10% 농도로 다시마 추출물을 첨가한 시험구는 오히려 관능이 감소하는 것으로 확인되었고, 조직감의 경우는 다시마 추출물 농도에 비례하는 것으로 확인되었다. 상기 측정 결과, 기호도가 가장 우수한 것으로 다시마 추출물을 5% 농도로 첨가한 침지액으로 침지시킨 것으로 평가되었다.As shown in FIG. 2, the odor, taste and overall acceptability of the sea tangle extract were increased when the sea tangle extract was added at a concentration of 5% in the sensory test. However, when the sea tangle extract was added at a concentration of 10% It was found that the sensory evaluation of the test group with kelp extract decreased and the texture of the kelp extract was proportional to the concentration of kelp extract. As a result of the measurement, it was evaluated that the best taste was obtained by immersing the sea tangle extract in an immersion liquid containing 5% concentration.
또한, 추출물 첨가량에 따른 항산화 활성은 구체적으로 DPPH radical 소거능(DPPH radical scavenging activity assay)으로 확인되었다.The DPPH radical scavenging activity assay (DPPH radical scavenging activity assay) was also confirmed.
구체적으로, 96 well plate에 각 농도별로 조제한 시료 100 ㎕와 에탄올에 용해된 0.4 mM DPPH (Sigma, USA)를 동량 첨가하여 30분간 암실에 방치한 후 Microplate Reader (Epoch, BioTek, USA)를 이용하여 540 nm에서 흡광도를 측정하였다. 추출물을 첨가하지 않은 경우를 대조군으로 평가하였다. 항산화 활성은 시료를 첨가하지 않은 음성대조군의 흡광도를 측정하여 다음 식으로 계산한 후 음성대조군에 대한 항산화활성을 백분율로 표시하여 비교하여 나타내었다. 각 시료에 대한 DPPH Free Radical 소거능을 3회 반복하여 측정하였다. 상기 측정결과를 도 3에 나타내었다.Specifically, 100 μl of each sample was added to 96-well plates, and 0.4 mM DPPH (Sigma, USA) dissolved in ethanol was added to the wells. The plate was incubated for 30 minutes in a dark room and then incubated with a microplate reader (Epoch, BioTek, USA) Absorbance was measured at 540 nm. And the case where the extract was not added was evaluated as a control. The antioxidant activity was measured by measuring the absorbance of a negative control to which no sample had been added, and the antioxidant activity of the negative control group was expressed as a percentage in comparison with the following equation. DPPH Free Radical scavenging ability of each sample was measured 3 times. The measurement results are shown in Fig.
[계산식 1][Equation 1]
DPPH radical scavenging activity (%) = ( ) x 100DPPH radical scavenging activity (%) = ( ) x 100
상기 도 3에 나타낸 바와 같이, 항산화효과를 검증한 결과 DPPH 라디컬 소거능은 대조구(81.85%)와 비교하여 5% 다시마추출물 처리구에서 상대적으로 다소 높은(83.45%) 경향을 보였고 그 외 1, 2, 10% 다시마추출물 처리구는 감소하였다. As shown in FIG. 3, DPPH radical scavenging activity was relatively higher (83.45%) in the treatment with 5% seaweed extract than in the control (81.85%), 10% kelp extract treatment decreased.
따라서, 상기 결과로부터, 다시마 추출물 5%가 첨가된 침지액을 이용하여 조미 명란을 제조하는 것이 가장 우수한 것으로 확인되었다.Therefore, it was confirmed from the above results that it is most preferable to prepare a seasoned starch using an immersion liquid to which 5% of seaweed extract is added.
Claims (7)
명란을 소금물에 염지한 후, 유수에서 세척하여 염지 명란을 준비하는 명란 준비 단계; 및
물에 상기 다시마 추출물을 5%(w/w) 농도로 첨가하여 제조한 다시마 추출물이 포함된 침지액 100 중량부에 상기 염지 명란 200 중량부를 침지하고 숙성시키는 조미 명란 제조단계
를 포함하는 조미명란 제조방법.Preparing a seaweed extract by adding an aqueous ethanol solution to the kelp powder and extracting it, followed by filtration, concentration under reduced pressure and drying;
A brightening preparation step in which bright coloring is salted with brine, and then washing is carried out in running water to prepare a bright coloring clear coloring; And
To 100 parts by weight of an immersion liquid containing sea tangle extract prepared by adding the above sea tangle extract to water at a concentration of 5% (w / w), 200 parts by weight of the above-
/ RTI >
상기 다시마 추출물 제조단계는 다시마 분말 100 중량부에 70% 에탄올 수용액 1,500 중량부를 첨가하고, 60℃ 내지 80℃ 조건에서 10분 내지 120분 동안 추출한 후, 여과, 감압농축 및 -20℃에서 동결건조과정을 수행하여 다시마 추출물을 제조하는 방법으로 수행하는 것을 특징으로 하는 조미명란 제조방법.The method according to claim 1,
The kelp extract is prepared by adding 1,500 parts by weight of 70% ethanol aqueous solution to 100 parts by weight of tallow powder, extracting the mixture at 60 to 80 ° C for 10 to 120 minutes, filtering, concentrating under reduced pressure and freeze- To thereby produce a seaweed extract. ≪ Desc / Clms Page number 20 >
상기 다시마 추출물 제조단계는 다시마 분말 100 중량부에 70% 에탄올 수용액 1,500 중량부를 첨가하고, 70℃ 조건에서 30분 동안 추출한 후, 여과, 감압농축 및 -20℃에서 동결건조과정을 수행하여 다시마 추출물을 제조하는 방법으로 수행하는 것을 특징으로 하는 조미명란 제조방법.3. The method of claim 2,
The kelp extract was prepared by adding 1,500 parts by weight of 70% ethanol aqueous solution to 100 parts by weight of kelp powder, extracting the mixture at 70 ° C for 30 minutes, filtering, concentrating under reduced pressure, and lyophilizing at -20 ° C, The method according to any one of claims 1 to 3,
상기 명란 준비 단계는 명란을 소금물에 48시간 동안 염지한 후, 유수에서 세척하여 염지 명란을 준비하는 방법으로 수행하는 것을 특징으로 하는 조미명란 제조방법.The method according to claim 1,
Wherein the preparation step is carried out by a method comprising salting the pungrang in salt water for 48 hours and washing the pungmul in the water to prepare a pungent pungmyeon.
상기 조미 명란 제조단계는 물에 상기 다시마 추출물을 5%(w/w) 농도로 첨가하여 제조한 다시마 추출물이 포함된 침지액 100 중량부에 상기 염지 명란 200 중량부를 침지시키고 4℃ 내지 6℃에서 40시간 내지 56시간 동안 숙성시키는 방법으로 수행하는 것을 특징으로 하는 조미명란 제조방법.The method according to claim 1,
The step of preparing the seasoned sea bream is carried out by immersing 200 parts by weight of the sea bream albumen in 100 parts by weight of an immersion liquid containing sea tangle extract prepared by adding the sea tangle extract at a concentration of 5% (w / w) And aging the mixture for 40 to 56 hours.
상기 조미 명란 제조단계는 물에 상기 다시마 추출물을 5%(w/w) 농도로 첨가하여 제조한 다시마 추출물이 포함된 침지액 100 중량부에 상기 염지 명란 200 중량부를 침지시키고 5℃에서 48시간 동안 숙성시키는 것을 특징으로 하는 조미명란 제조방법.6. The method of claim 5,
The preparation of the seasoned sea bream was carried out by immersing 200 parts by weight of the sea tangle base oil in 100 parts by weight of an immersion liquid containing sea tangle extract prepared by adding the sea tangle extract at a concentration of 5% (w / w) And aging the mixture.
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KR101534286B1 (en) | 2012-06-29 | 2015-07-07 | 주식회사 덕화푸드 | Composition of season for spawn of pollack with raw rice-wine and method for preparing salted pollack roe using thereof |
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KR100537814B1 (en) | 2001-12-21 | 2005-12-20 | 유겐카이샤 후지.바이오 겐큐쇼 | A soused roe of alaskan pollack-flavored seasoning and processed foodstuffs thereof |
KR20110105040A (en) | 2010-03-18 | 2011-09-26 | 유한회사 다원 | Seasoning laver including pollack roa powder |
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E902 | Notification of reason for refusal | ||
E601 | Decision to refuse application |