KR20190012690A - Analysis module - Google Patents

Abstract

Disclosed is an analysis module for preventing analysis specimens stored in each microchamber from being cross-contaminated. The analysis module includes: a plurality of microchambers; and a temperature-soluble analysis specimen body stored in each of the microchambers. According to an embodiment, the temperature-soluble analysis specimen body includes: an analysis mixture formed by mixing an analysis specimen with gelatin which maintains a gel state or film state at a relatively low temperature while being dissolved at a relatively high temperature; and a temperature-soluble coating layer coating the analysis specimen body and dissolved at a relatively high temperature. Therefore, the analysis specimen of the analysis specimen body is covered by the temperature-soluble coating layer, which maintains a gel state or film state at a relatively low temperature while being dissolved at a relatively high temperature when water or a water-based solution is supplied, and thus, the present invention is capable of preventing analysis specimens from evaporating in microchambers, preventing the analysis specimens from being mixed, and preventing pollutants from flowing into the analysis specimens.

Description

Analysis Module {ANALYSIS MODULE}

The present invention relates to an analytical module, and more particularly, to an analytical module processed so that the analytical sample contained in each of the microchambers is not cross-contaminated.

In general, the micro chamber plate is a container in which a microscopic reaction of several microliters or less occurs, and may be formed of a silicon wafer, glass, metal, plastic, or the like. The micro chamber plate is a plate in which micro chambers are two-dimensionally arranged, and is generally made of a transparent material for observing the reaction inside the micro chamber, and the other side is an injection port for injecting a sample.

On the other hand, as a method of measuring the amount of a gene, a real-time PCR (Polymerase Chain Reaction) method, which realizes a fluorescence value which is increased in proportion to the amount of a gene in real time PCR) method was developed. In the real-time PCR, the fluorescence value generated from the polymerase chain reaction product is measured for each cycle, and a cycle in which a fluorescence value of a certain amount or more is generated is confirmed by performing a PCR reaction, It can be quantitatively analyzed.

Various types of real-time PCR reaction devices for real-time PCR were proposed, and standard 96-well and 384-well plates were used as real-time PCR reaction devices capable of analyzing a large number of samples. A device has been proposed that can analyze 96 or 384 genes using. For example, Roche's real-time polymerase chain reaction apparatus has a problem that a relatively large amount of sample is required because the amount of the reaction sample is 10 to 50 μl.

In order to solve the above problems, various methods for simultaneously analyzing a large number of samples in a short time by reducing the amount of reactive samples using MEMS (Micro Electro Mechanical Systems) have been proposed. Accordingly, a method using a micro chamber plate It has been proposed.

The method of using the micro chamber plate can be largely composed of three steps of injecting the reaction sample into the micro chambers, sealing the micro chamber plate, and reaction and analysis steps.

Korean Registered Patent No. 10-1362905 (Apr. 201, 02. 07) (Micro chamber plate, manufacturing method thereof) Korean Patent No. 10-0719029 (2007. 05. 10.) (Micro-well plate) Korea Patent Publication No. 2015-0047598 (May 05, 2015) (Microwell plate)

SUMMARY OF THE INVENTION Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and it is an object of the present invention to provide a microchamber that can prevent the analytical sample in the microchambers formed in the microchamber plate from evaporating, And an analyzing module capable of blocking the inflow of the misty substance into the analytical sample in the microchamber.

In order to achieve the object of the present invention, an analysis module according to one embodiment includes: a micro chamber plate including a plurality of micro chambers; And a temperature-soluble analytical sample contained in each of the micro-chambers.

In one embodiment, the temperature-soluble analytical specimen comprises an analytical sample in contact with the bottom of each of the microchambers; And gelatin attached to the side surface of the microchamber while covering the analytical sample with a characteristic of being dissolved in a high temperature state while maintaining a gel state at a low temperature state.

In one embodiment, the temperature-soluble analytical sample may be composed of a mixture of analytical sample and gelatin having a property of maintaining a gel state at a low temperature state and dissolving at a high temperature state.

In one embodiment, the analytical sample for temperature-responsive analysis is prepared by mixing a hydrogel having a property of maintaining a solid state at room temperature and maintaining a liquid state at a temperature lower than the normal temperature and a temperature higher than the room temperature, An analytical mixture in contact with the inner surface of each of the microchambers; And gelatin attached to the inner surfaces of each of the microchambers while keeping the gel state at a low temperature and dissolving at a high temperature to cover the analytical mixture.

In one embodiment, the analytical sample may be in powder form.

In one embodiment, the analytical sample may comprise a primer.

In one embodiment, the analytical sample may comprise a probe.

According to this analysis module, the analytical sample of the temperature water soluble analytical sample accommodated in each of the micro chambers maintains the gel state or the film state at a relatively low temperature and the property of dissolving at a relatively high temperature in a state in which a solution containing water or water is supplied Water-soluble coating layer having a thickness of about 10 to 30 μm, so that the analytical sample in the microchambers can be prevented from evaporating, the analytical sample between the microchambers can not be mixed, You can block things.

1 is an exploded perspective view illustrating an analysis module according to an embodiment of the present invention.
2 is a cross-sectional view illustrating an example of a micro chamber of the PCR module shown in FIG.
3 is a cross-sectional view for explaining another example of the micro chamber of the PCR module shown in FIG.
4 is a cross-sectional view for explaining another example of the micro chamber of the PCR module shown in FIG.
5 is a plan view for explaining an analysis module according to another embodiment of the present invention. In particular, a microchamber plate is shown as an analysis module.
6 is a longitudinal sectional view for explaining an example of a micro chamber of the micro chamber plate shown in FIG.
7 is a longitudinal sectional view for explaining another example of the micro chamber of the micro chamber plate shown in FIG.
8 is a longitudinal sectional view for explaining another example of the micro chamber of the micro chamber plate shown in FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will now be described in more detail with reference to the accompanying drawings. The present invention is capable of various modifications and various forms, and specific embodiments are illustrated in the drawings and described in detail in the text. It should be understood, however, that the invention is not intended to be limited to the particular forms disclosed, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

Like reference numerals are used for like elements in describing each drawing. In the accompanying drawings, the dimensions of the structures are enlarged to illustrate the present invention in order to clarify the present invention.

The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component. The singular expressions include plural expressions unless the context clearly dictates otherwise.

In this application, the terms "comprises", "having", and the like are used to specify that a feature, a number, a step, an operation, an element, a part or a combination thereof is described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, components, parts, or combinations thereof.

Also, unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

1 is an exploded perspective view illustrating an analysis module according to an embodiment of the present invention. 2 is a cross-sectional view illustrating an example of a micro chamber of the PCR module shown in FIG. In particular, a PCR module is described as an analysis module.

1 and 2, an analysis module 100 according to an exemplary embodiment of the present invention includes a PCB substrate 110, a CMOS image sensor 120, a chamber member 130, a micro flow path member 140, And a switch 150. In this embodiment, the PCB substrate 110, the CMOS image sensor 120, the chamber member 130, the micro flow path member 140, and the membrane switch 150 can define a PCR module.

The PCB substrate 110 has a first center hole 112 having a rectangular shape and a rectangular shape formed in a central region, a first peripheral hole 114 formed on both sides of the first center hole 112, 2 peripheral holes 116. [

The CMOS image sensor 120 is inserted into the first center hole 112 formed in the PCB substrate 110.

The chamber member 130 is disposed on the CMOS image sensor 120 and inserted into the first center hole 112 formed in the PCB substrate 110. [ The chamber member 130 includes micro-chambers. The shapes, dimensions and number of the micro chambers may vary. Each of the microchambers is provided with a temperature-soluble analytical specimen 910.

The temperature water solubility analysis sample 910 includes a temperature water soluble coating layer 914 covering the analytical sample 912 and the analytical sample 912 and is contained in each of the micro chambers 112.

The analytical sample 912 is placed in contact with the bottom of each of the micro chambers 112. The analytical sample 912 may be a powder sample or a liquid sample. In this embodiment, the analytical sample 912 is a specific component for biological material analysis. That is, the analysis sample 912 refers to a primer, a probe, an antibody, an aptamer, a DNA or an RNA polymerase, and the like, as a component for quantitative or qualitative analysis of a specific biological substance such as protein, DNA, In particular, a component necessary for performing a real-time PCR, a constant temperature enzyme reaction, or an LCR (Ligase Chain Reaction).

The temperature water-soluble coating layer 914 maintains the gel state or the film state at a relatively low temperature and dissolves at a relatively high temperature in a state where a solution containing water or water is supplied. 112). That is, in this embodiment, the temperature water-soluble coating layer 914 is characterized in that when a solution containing water or water is supplied in a very dry state, it slowly dissolves at a low temperature (for example, at ordinary temperature) and rapidly dissolves at a high temperature . Accordingly, in a gel state or at a low temperature, the analytical sample 912 is sealed by the temperature water-soluble coating layer 914, and a solution containing water or water is supplied to the sample 912 at a high temperature, that is, And is melted under the conditions of a constant temperature enzyme reaction, LCR (Ligase Chain Reaction) and the like. Therefore, the analytical sample 912 can be used for a real-time PCR, a constant temperature enzyme reaction, or an LCR. The temperature water soluble coating layer 914 may comprise gelatin.

The micro flow path member 140 has a rectangular second center hole 141 formed in a central region with a loud rectangular shape and a first peripheral groove 142 formed on both sides of the second center hole 141, A curved first micro channel 144 connecting the second center hole 141 and the first peripheral groove 142, a second center hole 141 connecting the second center hole 141 and the second peripheral groove 142, And a second micro flow path 145 having a curved shape connecting the first micro flow path 143 and the second micro flow path 145. The second center hole 141 corresponds to the first center hole 112. And the chamber member 130 is exposed through the second central hole 141. The exposed chamber member 130 is connected to the first micro flow channel 144 and the second micro flow channel 145.

The membrane switch 150 has a rounded square shape and is disposed on the micro flow path member 140. The membrane switch 150 may comprise a transparent material.

The membrane switch 150 includes a push button 152 protruding upward in correspondence with the second center hole 141, a third hole 154 formed in correspondence with the first peripheral groove 142, And a fourth hole 156 formed corresponding to the second hole 143. [ The sample reagent can be injected through the third hole 154 and the sample reagent can be drained through the fourth hole 156. Further, when the pressing button 152 is pressed by the user, the sample reagent can be fully charged in the chambers of the chamber member 130 exposed through the second central hole 141 of the micro flow path member 140 have.

Although the first microchannel 144 and the second microchannel 145 connected to the second center hole 141 formed in the microchannel member 140 are formed in the microchannel member 140 in the present embodiment, The microfluidic channels may be formed in the membrane switch 150 corresponding to the first microchannel 144 and the second microchannel 145.

The cross-contamination does not occur since the analytical sample 912 contained in the chamber member 130 is coated by the temperature water-soluble coating layer 914 before the analysis module 100 is transferred or the PCR is performed in the analysis module 100 .

When the PCR is performed in the analysis module 100, if heat is applied from a lower portion of the chamber member 130 through a heater (not shown), the temperature water-soluble coating layer 914 coated on the chamber member 130 is immersed in water or water The solution containing it is melted in the supplied state, and a conventional PCR process proceeds. For example, when the PCR solution is injected into the inlet of the PCR module, the chambers of the chamber member 130 are filled with the inflow microfluidic channels.

The PCR solution filled in all of the chambers overflows and reaches the outflow through the outflow microchannels. The PCR solution reaching the outflow part can be leaked to the outside.

As described above, according to the present invention, since the analytical sample is coated with the temperature water-soluble coating layer such as gelatin, the analytical sample can be prevented from being evaporated.

In addition, even if the analysis module is transferred, it is possible to prevent the cross contamination in which the analysis sample of the specific chamber is mixed with the analysis sample of the adjacent chamber and the analysis sample of the adjacent chamber is mixed with the analysis sample of the specific chamber.

3 is a cross-sectional view for explaining another example of the micro chamber of the PCR module shown in FIG. In particular, an example in which the reagent is coated with gelatin after mixing with gelatin is shown.

Referring to FIG. 3, the temperature-soluble analytical specimen 920 includes an analytical mixture 922 and a temperature-soluble coating layer 924.

The analytical mixture 922 is composed of a mixture of gelatin having a property of maintaining a gel state or a film state at a low temperature relative to the analytical sample and dissolving at a relatively high temperature.

The temperature water-soluble coating layer 924 is coated on the analysis mixture 922 with the property that the solution containing water or water is dissolved at a relatively high temperature in a state of being supplied. The temperature water soluble coating layer 924 may comprise gelatin.

Thus, in the low temperature state, the analytical sample is attached to the bottom of each of the microchambers by gelatin, and is attached to the side wall of the chamber by the temperature water-soluble coating layer 924. On the other hand, it is dissolved under a condition of high temperature, that is, a real-time PCR, a constant temperature enzyme reaction, or an LCR (Ligase Chain Reaction). Therefore, the analytical sample mixed in the analytical mixture 922 can be used for a real-time polymerase chain reaction, a cryogenic enzyme reaction, or an LCR.

As described above, the analytical sample is mixed with gelatin to define the analytical mixture, and the analytical mixture is coated with the temperature water-soluble coating layer such as gelatin, so that the analytical sample can be prevented from being evaporated.

In addition, even if the analysis module is transferred, it is possible to prevent the cross contamination in which the analysis sample of the specific chamber is mixed with the analysis sample of the adjacent chamber and the analysis sample of the adjacent chamber is mixed with the analysis sample of the specific chamber.

4 is a cross-sectional view for explaining another example of the micro chamber of the PCR module shown in FIG. In particular, an example in which a gelatin is coated after the hydrogel is mixed with a reagent is shown.

Referring to FIG. 4, the temperature-soluble analytical specimen 930 includes an analytical mixture 932 and a temperature-soluble coating layer 934.

The analytical mixture 932 is prepared by mixing a hydrogel having a property of maintaining a solid state at room temperature and maintaining a liquid state at a low temperature lower than the normal temperature and a high temperature higher than the normal temperature, Touch the side. Generally, a hydrogel means a polymer structure having a three-dimensional network structure containing an aqueous phase, and is formed by covalent bonding or noncovalent bonding between hydrophilic polymers. In this embodiment, the hydrogel 322a maintains a solid state at room temperature and has a property of maintaining a liquid state at a low temperature lower than the normal temperature and a high temperature higher than the normal temperature.

The temperature water-soluble coating layer 934 maintains the gel state at a low temperature and dissolves at a high temperature in a state where a solution containing water or water is supplied. The analytical mixture 932, that is, the analytical sample mixed in the hydrogel And is attached to the inner surface of each of the micro-chambers.

Accordingly, in the low-temperature state, the analytical sample is attached to the bottom of each of the micro chambers by the gelatin gelatin 324, and a high temperature state, that is, a real-time polymerase chain reaction, ), And the like. Therefore, the analytical sample mixed in the analytical mixture 932 can be used for real-time polymerase chain reaction, cryogenic enzyme reaction, or LCR.

As described above, the analytical sample is mixed with the hydrogel to define the analytical mixture, and the analytical mixture is coated with the temperature water-soluble coating layer such as gelatin, so that the analytical sample can be prevented from being evaporated.

In addition, even if the analysis module is transferred, it is possible to prevent the cross contamination in which the analysis sample of the specific chamber is mixed with the analysis sample of the adjacent chamber and the analysis sample of the adjacent chamber is mixed with the analysis sample of the specific chamber.

5 is a plan view for explaining an analysis module according to another embodiment of the present invention. 6 is a longitudinal sectional view for explaining an example of a micro chamber of the micro chamber plate shown in FIG. In particular, a microchamber plate is shown as an analysis module.

Referring to FIGS. 5 and 6, the analysis module 200 according to another embodiment of the present invention includes a micro chamber plate 210 and a temperature-soluble analytical specimen 220. In this embodiment, the analysis module is a PCR module, and the chambers are micro chambers employed in a PCR module.

The micro chamber plate 210 includes a plurality of micro chambers 212. The micro chambers 212 are generally arranged in n rows and m columns, and the number of micro chambers 212 may be variously formed such as 6, 12, 24, 48, 96, 384, 1536, 1 shows a microchamber plate 210 of 384 microchambers (26 * 24). The micro chamber plate 210 according to the present embodiment is not limited to this, and various shapes and dimensions may be used.

The micro chamber 212 is a part for defining a space for forming cell aggregation masses. That is, on the upper surface of the micro chamber plate 210, a micro chamber 212 for defining a recess is formed, and this recess is a space.

The micro chamber 212 may be formed to have a width of 0.3 to 3 mm and a depth of 0.5 to 5 mm. In this embodiment, a very large number of microchambers can be formed in the microchamber plate 210, so that analysis can be performed simultaneously, and the depth is shallow, which leads to a good thermal conduction performance, thereby reducing the analysis time and improving the accuracy of the analysis. In practice, 24,576 microchambers 212 having a width of 0.3 to 2.25 mm can be formed on a standard 80 mm x 25 mm body, and are suitable for the reaction of samples having a small amount of 0.1 to 5 m or less, (210) is advantageous in that a large number of genes can be simultaneously quantitatively analyzed using a small amount of reaction solution or a small amount of reaction powder. Here, as a sample, a specimen for quantitative or qualitative analysis may be used alone according to the micro chamber plate 210 manufactured according to the present invention, or a sample for real-time PCR (real-time PCR) Can be used. Components required for real-time PCR include reaction buffer solution, MgCl ₂ , four kinds of dNTPs, DNA or RNA polymerase, and the like, but not limited thereto, The components required for the reaction can be used without limitation. In addition, pyrophosphatase, pyrophosphatase, and the like may be further included for a hot start reaction of the real-time PCR.

The opening of the micro chamber 212 of this embodiment may have a substantially square shape when viewed in a plan view. As described above, by making the opening not a circular shape but a polygonal shape, the volume of the opening side portion can be increased. The shape of the opening has the effect of increasing the volume if it has a polygonal shape, but it is particularly preferable that the shape of the opening is a square, a pentagon, or a hexagon because the capacity is larger than that of the circular shape. In particular, in the micro chamber plate 210 having the number of the micro chambers 212 of 384 or more, the effect of increasing the capacity by making the opening 3 polygonal is large.

The temperature water solubility assay specimen 220 includes an analytical sample 222 and a temperature water soluble coating layer 224 and is contained in each of the micro chambers 212.

The analytical specimen 222 is placed in contact with the bottom of each of the micro-chambers 212. The analytical sample 222 may be a powder sample or a liquid sample. In this embodiment, the temperature-soluble analysis specimen 220 is a specific component for biological material analysis. That is, the temperature water solubility analyte sample 220 may be a primer, probe, antibody, aptamer, DNA or RNA polymerase, or the like as a component for quantitative or qualitative analysis of a specific biological substance such as protein, And specifically refers to a component necessary for performing a real-time PCR, a constant temperature enzyme reaction, or an LCR (Ligase Chain Reaction).

The temperature-soluble coating layer 224 maintains the gel state at a low temperature and dissolves at a high temperature in a state where a solution containing water or water is supplied. The temperature-soluble coating layer 224 covers the analytical sample 222, . Accordingly, in the low temperature state, the analytical sample 222 is sealed by the temperature-water-soluble coating layer 224 in the gel state, and the high-temperature state, that is, the real-time PCR reaction, the isothermal enzyme reaction, or the LCR (Ligase Chain Reaction) Is dissolved under the conditions under which it is made. Therefore, the assay sample 222 can be used for a real-time PCR, a constant temperature enzyme reaction, or an LCR. In this embodiment, the temperature-soluble coating layer 224 may comprise gelatin.

As described above, according to the present invention, since the analytical sample is coated with the temperature water-soluble coating layer such as gelatin, the analytical sample can be prevented from being evaporated.

In addition, even if the analysis module is transferred, it is possible to prevent the cross contamination in which the analysis sample of the specific chamber is mixed with the analysis sample of the adjacent chamber and the analysis sample of the adjacent chamber is mixed with the analysis sample of the specific chamber.

7 is a longitudinal sectional view for explaining another example of the micro chamber of the micro chamber plate shown in FIG.

Referring to FIG. 7, the temperature water solubility analyte sample 320 is a mixture of the analytical sample 322 and the gelatin 324 that maintains a gel state at a low temperature and melts at a high temperature.

The analytical sample 322 is attached to the bottom of each of the micro-chambers 112 by the gelatinous gelatin 324 at a low temperature, and the analytical sample 322 is attached to the bottom of each of the micro-chambers 112 at a high temperature, Or LCR (Ligase Chain Reaction). Therefore, the analytical sample 322 can be used for a real-time PCR, a constant temperature enzyme reaction, or an LCR.

As described above, since the analytical sample is mixed with the gelatin, the analytical sample can be prevented from being evaporated. In addition, even if the analysis module is transferred, it is possible to prevent the cross contamination in which the analysis sample of the specific chamber is mixed with the analysis sample of the adjacent chamber and the analysis sample of the adjacent chamber is mixed with the analysis sample of the specific chamber.

8 is a longitudinal sectional view for explaining another example of the micro chamber of the micro chamber plate shown in FIG.

Referring to FIG. 8, the temperature-soluble analyte sample 420 includes an analytical mixture 422 and a temperature-soluble coating layer 424.

The analytical mixture 422 is a mixture of the hydrogel 422a and the analytical sample 422b having a property of maintaining a solid state at room temperature and maintaining a liquid state at a temperature lower than the normal temperature and a temperature higher than the normal temperature, Lt; RTI ID = 0.0 > microchambers. ≪ / RTI > Generally, a hydrogel means a polymer structure having a three-dimensional network structure containing an aqueous phase, and is formed by covalent bonding or noncovalent bonding between hydrophilic polymers. In this embodiment, the hydrogel 422a maintains a solid state at room temperature, and has a property of maintaining a liquid state at a low temperature lower than the normal temperature and a high temperature higher than the normal temperature.

The temperature water-soluble coating layer 424 maintains a gel state at a low temperature state and dissolves at a high temperature in a state in which a solution containing water or water is supplied, and is mixed with the analytical mixture 422, that is, the hydrogel 422a Chambers 422a and 422b, respectively. In this embodiment, the temperature-soluble coating layer 424 may comprise gelatin.

Accordingly, in the low-temperature state, the analytical sample 422b is attached to the bottom of each of the micro chambers by the gel-state temperature water-soluble coating layer 424, and the high-temperature state, that is, real- LCR (Ligase Chain Reaction), and the like. Therefore, the analytical sample 422b can be used for a real-time PCR, a constant temperature enzyme reaction, or an LCR.

As described above, since the analytical sample is mixed with the hydrogel and placed in the microchamber and then coated with the water-soluble coating layer such as gelatin, the analytical sample can be prevented from being evaporated. In addition, even if the analysis module is transferred, it is possible to prevent the cross contamination in which the analysis sample of the specific chamber is mixed with the analysis sample of the adjacent chamber and the analysis sample of the adjacent chamber is mixed with the analysis sample of the specific chamber.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined in the appended claims. You will understand.

100, 200: Analysis module 110: PCB substrate
120: CMOS image sensor 130: chamber member
140: Micro-flow passage member 150: Membrane switch
210: Micro chamber plate 222, 322, 422, 912: Analytical sample
220, 320, 420, 910, 920, 930:
224, 424, 914, 924, 934: temperature water-soluble coating layer
422, 922, 932: Analysis mixture

Claims (16)

A plurality of microchambers; And
And a temperature-soluble analytical sample contained in each of the micro-chambers. The method according to claim 1, wherein the temperature-
An analytical sample in contact with the bottom of each of the microchambers; And
A temperature-soluble coating layer covering the analytical sample at a relatively low temperature, the gel layer having a property of maintaining a gel state or film state and dissolving at a relatively high temperature. The method according to claim 1, wherein the temperature-
An analytical mixture consisting of a mixture of gelatin having a property of maintaining a gel state or a film state at a low temperature relative to an analytical sample and dissolving at a relatively high temperature; And
And a temperature-soluble coating layer coated on the analysis mixture with properties dissolving at relatively high temperatures. The assay module of claim 3, wherein the gelatin is further coated on the inner surface of each of the microchambers. The method according to claim 1, wherein the temperature-
An analytical mixture in which a hydrogel having a property of maintaining a solid state at a room temperature relative to an analytical sample and maintaining a liquid state at a low temperature atmosphere lower than the normal temperature and a high temperature atmosphere higher than the room temperature; And
And a temperature-soluble coating layer coated on the analysis mixture with properties dissolving at relatively high temperatures. 6. The assay module of claim 5, wherein the temperature-soluble coating layer is further coated on the inner surface of each of the microchambers. 6. An assay module according to any one of claims 2, 3 and 5, wherein the temperature-soluble coating layer comprises gelatin. The analysis module according to any one of claims 2, 3 and 5, wherein the analytical sample is in powder form. The analysis module according to any one of claims 2, 3 and 5, wherein the analysis sample comprises a primer. The analysis module according to any one of claims 2, 3, and 5, wherein the analysis sample includes a probe. The analysis module of claim 1, wherein the microchambers are formed in a chamber member disposed on a CMOS image sensor. 12. The method of claim 11,
A PCB substrate comprising a CMOS image sensor and a first central hole formed for insertion of the chamber member; And
A second center hole formed corresponding to the first center hole, a first peripheral groove and a second peripheral groove formed on both sides of the second center hole, A first microchannel, and a second microchannel connecting the second center hole and the second peripheral groove. 13. The analysis module according to claim 12, further comprising a membrane switch disposed on the micro channel member and including a push button protruded corresponding to the second center hole. 14. The membrane switch according to claim 13, wherein the membrane switch further includes a third hole corresponding to a first peripheral groove of the micro flow path member and a fourth hole corresponding to a second peripheral groove of the micro flow channel member,
The PCR solution is injected through the third hole,
Wherein the PCR solution that overflows after the PCR solution is filled in the microchamber flows out through the fourth hole. The analysis module of claim 1, wherein the microchambers are employed in a micro chamber plate to define a space for forming cell aggregation masses. 16. The analysis module of claim 15, wherein the number of microchambers is any one of 6, 12, 24, 48, 96, 384 and 1536.

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