TW201732043A - Multiplex slide plate device and operation method thereof - Google Patents

Abstract

A multiplex slide plate device and an operation method thereof are provided. The multiplex slide plate device includes a slide plate and a sacrificial layer. The slide plate has reaction vessels arranged in an array, and each reaction vessel has an opening portion and a bottom portion. The sacrificial layer has a microfluidic channel, and the microfluidic channel has an injection channel, a main channel and a distal channel connects each other. The sacrificial layer is assembled to the slide plate, wherein the main channel faces the opening portion. A sample solution is injected into the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into each reaction vessel while flowing through the main channel.

Description

Multi-work test strip device and its operation method

The invention relates to a multiplex test strip device and a method for the same, which are applied to molecular biological detection, and in particular to a multiplexer for polymerase chain reaction (PCR) which can be pre-filled with a polymerase chain reaction reagent. Test strip device and its operation method.

In the field of molecular bioassays, it is often necessary to perform a test of multiple targets for one sample. For example, polymerase chain reaction detection is used to test the genotype of several single nucleotide polymorphisms (SNPs) in a sample, or to test the degree of expression of several genes in a sample. At this point, it would be necessary to have a test panel consisting of several DNA or RNA assays. In the PCR test, each group of test reagents includes at least two specific DNA primer molecules (some PCR assays additionally include target-specific reporter probes), and the pair of primers must be correctly and correctly To mix the DNA template extracted in the test sample (sample), it is possible to check the presence or amount of a specific DNA target in the sample.

Traditionally, primer pairs and samples were placed in the same reaction vessel for PCR. The general placement method is usually to manually transfer the primer pair, the enzyme and the dNTP mixture and the buffer reagent and the sample through a pipette into the reaction container by a pipette. The most common carrier container is a 96-well plate. Using the aforementioned placement method, the PCR detection requires at least two rounds (two times) of pipette pipetting operation, one of which adds the sample to the reaction vessel and the other adds the primer pair to the reaction vessel. For example, assuming a test set to check 36 targets in a sample, at least 36 pipetting operations are required to add the respective primer pairs to 36 different reaction vessels. In addition, 36 pipetting operations are required. A sample was added to each of the above reaction vessels. This type of operation is not only complicated and error-prone, but also requires a lot of manpower.

If the primer pair is prefilled in an individual reaction vessel, the experiment operator only needs to add the sample to the already prefilled vessel. In the previous example, testing 36 targets for one sample would require only 36 pipetting operations to add the sample to the pre-filled 36 reaction vessels. In addition, the volume of the reaction vessel can be simultaneously reduced to a range of about nano-liter to save the amount of detection reagent used. Under this modification, a 96-well plate, which is commonly used as a carrying container, will be changed to a microtiter plate similar to a test piece.

However, the size and volume of the reaction vessel (also known as a microwell or nanowell) of a microtiter plate is too small to manually fill the primer pairs or samples used while avoiding cross-contamination between adjacent reaction vessels (ie, The primer pair is dissipated from one well to the other, and therefore, special microfluidic dispensing techniques are required. In more detail, the primer pair is provided in advance to each nanowell and the primer is fixed to the surface of the nanowell. The user can then add the sample to each reaction vessel by a single pipetting operation or through a single microfluidic channel without worrying about the escape of the primer from one well to the other, minimizing cross-contamination between the well and the well.

For subsequent sample testing, a predetermined amount of sample must be filled in each reaction vessel. Conventionally, samples are added one by one to the reaction well using a pipette or needle dispenser. However, as the volume of the reaction vessel becomes smaller and the closer the distance between the wells, the dispensing of the dispenser or path of a particular mechanical mechanism, individually delivered to each reaction vessel, is complex and time consuming. If a special stripping device design is used to achieve a single pipetting operation or to add samples to each reaction vessel through a single microfluidic channel, the human manipulation required to prepare the detection reagents for the polymerase chain reaction can be greatly simplified, and the filling can be increased. Sample convenience.

The present invention provides a multiplex test strip device and method of operation thereof, suitable for molecular bioassays; in particular, for polymerase chain reaction; and more particularly, for real-time polymerase chain reaction. By the multiplex test strip device of the present invention and the method of operating the same, the sample can be quickly and uniformly loaded into each reaction container of the test piece, and all the reaction containers can be filled in a short time by a single back pipetting operation. .

The invention provides a multiplex test strip device comprising a test piece and a sacrificial layer. The test piece has a plurality of reaction vessels, a first injection hole and a first vent hole. The reaction vessels are arranged in an array, each reaction vessel having an opening and a bottom. The sacrificial layer has micro flow passages having injection passages, main flow passages, and end flow passages that communicate with each other. The sacrificial layer is adapted to be assembled with a test piece in which the main flow path is assembled facing the opening. A sample solution is injected from the first injection hole to the injection flow path to flow the sample solution from the injection flow path through the main flow path to the end flow path, wherein the sample solution is loaded into each reaction container as it flows through the main flow path.

In an embodiment of the invention, the multiplex test strip device further includes a housing for accommodating the test strip and the sacrificial layer, the housing having a second injection hole and a second vent hole, wherein the second injection is sequentially The hole and the first injection hole inject the sample solution into the injection flow channel, so that the sample solution flows from the injection flow channel through the main flow channel to the end flow channel, wherein the sample solution is loaded into each reaction container when flowing through the main flow channel .

In an embodiment of the invention, the material of the housing comprises a thermally conductive material.

In an embodiment of the invention, the housing includes an upper cover and a bottom plate, the upper cover is adapted to be assembled with the bottom plate, and the upper cover has a recess for receiving the test piece and the sacrificial layer, and the second injection hole and the second exhaust hole are located In the upper cover.

In an embodiment of the invention, the material of the test piece comprises a light transmissive material.

In an embodiment of the invention, the light transmissive material comprises polycarbonate or polymethyl methacrylate.

In an embodiment of the invention, the material of the sacrificial layer comprises a wax.

The invention provides a multiplex test strip device comprising a test piece and a sacrificial layer. The test piece has a plurality of reaction vessels, and the reaction vessels are arranged in an array, each reaction vessel having an opening and a bottom. The sacrificial layer has micro flow passages having injection passages, main flow passages, and end flow passages that communicate with each other. The sacrificial layer is adapted to be assembled with a test piece in which the main flow path is assembled facing the opening. The sample solution is injected into the injection flow path such that the sample solution flows from the injection flow path through the main flow path to the end flow path, wherein the sample solution is loaded into each reaction container as it flows through the main flow path.

In an embodiment of the invention, the device further includes a housing for accommodating the test piece and the sacrificial layer, the housing having an injection hole and a vent hole, wherein the sample solution is injected from the injection hole to the injection flow path to make the sample solution Flows from the injection channel through the main flow path to the end flow path, wherein the sample solution is loaded into each reaction vessel as it flows through the main flow path.

In an embodiment of the invention, the material of the housing comprises a thermally conductive material.

In an embodiment of the invention, the housing includes an upper cover and a bottom plate, and the upper cover is adapted to be assembled with the bottom plate, and the upper cover has a recess for receiving the test piece and the sacrificial layer, and the injection hole and the exhaust hole are located in the upper cover.

In an embodiment of the invention, the material of the test piece comprises a light transmissive material.

In an embodiment of the invention, the light transmissive material comprises polycarbonate or polymethyl methacrylate.

In an embodiment of the invention, the material of the sacrificial layer comprises a wax.

The invention provides a method for operating a multiplex test strip device, comprising assembling a multiplex test strip device comprising a test strip, a sacrificial layer and a housing for accommodating the test strip and the sacrificial layer. The test piece has a plurality of reaction vessels arranged in an array, each reaction vessel has an opening portion and a bottom portion, and the sacrificial layer has a micro flow channel having intervening injection flow channels, main flow channels and end flow channels, wherein The flow path is assembled facing the opening. Next, the sample solution is injected into the injection flow path through the injection hole of the housing, so that the sample solution flows from the injection flow path through the main flow path to the end flow path, wherein the sample solution is loaded into each of the main flow channels. In the reaction vessel. Thereafter, oil is injected into the injection flow path through the injection hole of the casing to allow oil to flow from the injection flow path through the main flow path to the end flow path, wherein the oil is excluded from being loaded in the reaction container when flowing through the main flow path Sample solution. Next, heating is performed to melt the sacrificial layer, and the melted sacrificial layer is mixed with the oil.

In an embodiment of the invention, the oil comprises mineral oil or eucalyptus oil.

In an embodiment of the invention, the material of the housing comprises a thermally conductive material.

In an embodiment of the invention, the material of the test piece comprises a light transmissive material.

In an embodiment of the invention, the light transmissive material comprises polycarbonate or polymethyl methacrylate.

In an embodiment of the invention, the material of the sacrificial layer comprises a wax.

Based on the above, the present invention provides a multiplex test strip device and a method of operating the same that allows a sample to be quickly and uniformly loaded into each reaction vessel of a test piece while flowing through a main flow path of the sacrificial layer, and then by oil The sample solution not loaded in the reaction vessel is excluded. In this way, all of the reaction vessels can be filled in a short period of time with a single back pipetting operation, thereby simplifying the experimental operation and saving time.

On the other hand, the test piece and the sacrificial layer are separated by a distance of at least about 10 μm, and the sacrificial layer has a certain thickness. During the experiment of the polymerase chain reaction, heating was performed to melt the sacrificial layer, and the melted sacrificial layer was mixed with the oil to further have a distance of about 600 μm between the test piece and the bottom plate to allow the reaction to proceed smoothly. Since it is not necessary to add an excessive amount of sample, a certain distance can be maintained between the test piece and the bottom plate, thereby saving the amount of sample added.

The above described features and advantages of the invention will be apparent from the following description.

The invention provides a multiplex test strip device and an operation method thereof, which can be widely applied to different types of reaction tests. In the following, the definitions of the nouns used in the text of the specification are first explained.

"Reagent" may refer to a formulation or formulation of several ingredients for a particular test/test. For example, in a polymerase chain reaction assay, the detection reagent includes a pair of primers, enzymes, dNTPs, fluorescent reporters, salts, and the like. In the application, different primer pairs and fluorescent reporters may be added to the reaction vessel first, followed by the sample mixed with enzyme, dNTP and other additives and added to the reaction vessel.

"Sample" refers to the nucleic acid sample being tested. For example, the sample may be a nucleic acid fragment (including DNA or RNA, etc.) extracted from a source of blood, tissue, saliva or the like.

"Analysis" or "test" may refer to one or more experiments or test items performed on the same sample. For example, PCR is used to detect 300 single nucleotide polymorphism (300 SNP) genotypes for nucleic acid samples, including several PCR assays, by examining each gene for each single nucleotide position (SNP) Type (A, T, C, G). For example, real-time fluorescent quantitative PCR is used to determine the amount of nucleic acid for a particular sequence.

The "sample solution" refers to a mixture or a mixed solution of the above "sample" and a master mix.

The "reaction vessel" may refer to each tube of the tray, each reaction tube, a well or well of a microtiter plate, or a pit/hole of a test piece or array plate. As used herein, "test strip", "test strip", "detection array plate" or "detection plate" may refer to the same substrate or substrate that houses the aforementioned reaction vessel.

When the volume of liquid in the container is reduced to a certain extent, the flow of liquid in the container is primarily controlled by the adhesion of the surface rather than gravity. If the volume of liquid in the container is only a few nanoliters, the liquid has a high surface adhesion which will adhere to the container (Naijing), that is, the liquid can be stably attached to the bottom of the container as an adhesive or On the wall of the container.

Preferably, the reaction vessel can be a test coupon or a single reaction well or well that detects the array panel. As previously discussed, it is preferred to use a smaller volume of reaction vessel, the size of which varies, for example, from a few nanoliters to several hundred nanoliters.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a block diagram showing the structure of a multiplex test strip apparatus according to a first embodiment of the present invention.

Referring to FIG. 1, the multiplex test strip device includes a test strip 10, a sacrificial layer 20, and a housing 30, wherein the housing 30 can be used to accommodate the test strip 10 and the sacrificial layer 20. Hereinafter, the structure of the test piece 10 will be described in detail with reference to FIGS. 2 and 3.

Figure 2 is a schematic view showing the structure of a test piece according to a first embodiment of the present invention.

Referring to FIG. 2, the test strip 10 has a detection area 100, and the area of the detection area 100 is, for example, 22.5 mm × 22.5 mm. The detection zone 100 contains a plurality of reaction vessels 102, and the reaction vessels 102 are arranged in an n x n array. Further, the test piece 10 further has an injection hole 12 and an exhaust hole 14. In the present embodiment, the size of the test piece 10 is, for example, 42 mm × 36 mm × 2 mm. In more detail, the material of the test piece 10 may include a light transmissive material, and the light transmissive material is, for example, polycarbonate (PC) or polymethyl methacrylate (PMMA).

Referring again to FIG. 2, from the cross-sectional view of the test piece 10 (the right side portion of FIG. 2), each of the reaction vessels 102 has a wider opening portion 102a and a narrower bottom portion 102b. In the present embodiment, the depth of each of the reaction vessels 102 is, for example, 100 μm (d1), the size of the opening portion 102a is, for example, 200 μm (L1) × 185 μm (W1), and the size of the bottom portion 102b is, for example, 106.74 μm ( L2) × 91.74 microns (W2). The spacing (P1) between the reaction vessels 102 ranges, for example, from 25 micrometers to 40 micrometers. The angle θ of the inclined side walls of the reaction vessel 102 is, for example, 90 to 140 degrees, preferably between 100 and 135 degrees, more preferably between 110 and 120 degrees. Each reaction vessel 102 can hold, for example, 2.1 nanoliters of sample solution.

Figure 3 is a cross-sectional view showing a reaction vessel of a test piece according to a first embodiment of the present invention.

Referring to FIG. 3, the reaction vessel of the test strip 10 can be designed to have a different shape or arrangement. For example, the reaction vessels 160a and 160b are grooves formed in the test piece 10 but do not penetrate the test piece 10. The reaction vessels 160b, 160d, and 160f have inclined side walls. The reaction vessels 160c, 160d, 160e, and 160f penetrate the test piece 10 and have two open ends on the top and bottom surfaces of the test piece 10. The sample liquid can be stabilized in the reaction vessels 160c, 160d, 160e, and 160f due to capillary action. The reaction vessel 160d penetrates the test piece 10 and has two open ends on the top and bottom surfaces of the test piece 10, and has two open ends to which the inclined side walls are joined.

However, the structural diagrams shown in FIG. 2 and FIG. 3 are only used to illustrate the embodiment. The shape, size or number of the reaction vessel of the present invention is not limited thereto, and the cross-sectional shape of the reaction vessel may also be, for example. Round, square or polygonal.

In general, the primer is dissolved in an aqueous solvent or solution, and the test piece of the present invention can be designed such that the inner and bottom surfaces of the reaction vessel are hydrophilic, and each reaction vessel is hydrophobic. The reagent or probe will only adhere to the hydrophilic region, that is, only to the inner and bottom surfaces of the reaction vessel. Each reaction vessel may have a size of less than 1 mm. With this size and size, a small amount of sample liquid can overflow and fill a large number of reaction vessels in 10 seconds, which significantly improves sample loading efficiency.

4 is a schematic view showing the application of a multiplex test strip device to a sample solution according to a first embodiment of the present invention. Next, in the following, the structure of the multiplex test strip apparatus of the first embodiment of the present invention and its application to the loading of the sample solution will be described with reference to Figs. 1, 2 and 4.

Referring to FIG. 1, the sacrificial layer 20 has microchannels 28, and the microchannels 28 have injection channels 22, main channels 24, and end channels 26 that communicate with each other. In the present embodiment, the material of the sacrificial layer 20 may include wax, and thus, it may be melted when heated to about 60 ° C in the polymerase chain reaction. However, the invention is not limited thereto, and any material having a fusible temperature range of from room temperature to 60 ° C can be used, and a material having a fusible temperature of about 60 ° C is preferably used. In more detail, the size of the sacrificial layer 20 is, for example, 37.6 mm × 39.6 mm × 1.3 mm, the depth of the micro flow path 28 is, for example, 0.12 mm, and the size of the main flow path 24 is, for example, 22.5 mm × 22.5 mm.

1 and 2, the sacrificial layer 20 is adapted to be assembled with the test piece 10, wherein the main flow path 24 is assembled facing the opening portion 102a of the reaction container 102 in the test piece 10. Referring to FIG. 1 , FIG. 2 and FIG. 4 simultaneously, the sample solution can be injected from the injection hole 12 of the test piece 10 to the injection flow path 22 so that the sample solution flows from the injection flow path 22 through the main flow path 24 to the end flow path 26 . Wherein the sample solution is loaded into each of the reaction vessels 102 of the test strip 10 as it flows through the main flow path 24 (the flow direction of the sample solution is as indicated by the dashed arrow in FIG. 4).

In FIG. 2, the injection hole 12 and the vent hole 14 of the test piece 10 are arranged diagonally, but the invention is not limited thereto, and the injection hole 12 and the vent hole 14 may be arranged according to a sacrificial layer. The arrangement of the injection flow path 22 and the end flow path 26 of 20 is adjusted as long as the sample solution can be sufficiently extended during the flow.

As shown in FIG. 1, the housing 30 can include an upper cover 40 and a bottom plate 50, wherein the upper cover 40 is adapted to be assembled with the bottom plate 50. In more detail, the size of the upper cover 40 is, for example, 45 mm × 43 mm × 5 mm, and the size of the bottom plate 50 is, for example, 42 mm × 40 mm × 2 mm. In the embodiment, the upper cover 40 may have a recess for receiving the test strip 10 and the sacrificial layer 20, and the injection hole 42 and the exhaust hole 44 may be located in the upper cover 40. However, the structure of the casing 30 in the present invention is not limited thereto, and any other structure capable of accommodating the test piece 10 and the sacrificial layer 20 may be used. For example, the housing 30 may also be an integrally formed structure having a cassette that can be opened and closed to be placed into the test strip 10 and the sacrificial layer 20. Further, the test strip 10 and the sacrificial layer 20 may be housed in the casing 30 using the guide groove advancing structure.

In more detail, the housing 30 has a heat conducting effect in the polymerase chain reaction, and the material thereof may include a heat conductive material such as a metal such as aluminum or copper, graphite or a wafer, but the invention is not limited thereto. . Further, the casing 30 further has the effect of isolating the test piece 10 and the sacrificial layer 20 from the outside to prevent the reaction from being affected.

In the present embodiment, the housing 30 may include an identification label (not shown), and when the multiplex test strip device of the present invention is applied to an instrument (for example, a thermal cycler) provided with the label reading device, the label reading is performed. The device can read the label on the housing 30, wherein the label is, for example, a handwritten mark, a bar code or other mark, but the invention is not limited thereto, and can be selected according to the requirements and the label reading device used together. Label label.

Referring to FIG. 1 , FIG. 2 and FIG. 4 simultaneously, the sample solution can be injected from the injection hole 42 of the casing 30 and the injection hole 12 of the test piece 10 to the injection flow channel 22 in order to flow the sample solution from the injection flow channel 22 . The main flow path 24 to the end flow path 26, wherein the sample solution is loaded into each of the reaction vessels 102 of the test strip 10 as it flows through the main flow path 24 (the flow direction of the sample solution is as indicated by the dotted arrow in FIG. 4) ). In addition, as shown in FIG. 4, the test strip 10 may further have a double-stage structure 16 at the edge thereof, and a space 60 may be formed between the double-stage structure 16 and the sacrificial layer 20, the upper cover 40 and the bottom plate 50, and the space 60 has steam prevention. The role of bubbles. In this embodiment, the double-order structure 16 may have a first order x and a second order y, wherein the thickness ratio of the first order x and the second order y is, for example, 1:1, and the first order x and the second order The thickness of y is, for example, 1 mm, respectively, and the total thickness is, for example, 2 mm.

In FIG. 1, the injection hole 42 and the vent hole 44 of the upper cover 40 are arranged diagonally, but the invention is not limited thereto. The arrangement of the injection hole 42 and the exhaust hole 44 depends on the arrangement of the injection hole 12 and the exhaust hole 14 of the test piece 10. That is, as described above, it can be adjusted according to the arrangement of the injection flow path 22 and the end flow path 26 of the sacrificial layer 20 as long as the sample solution can be sufficiently extended during the flow.

Figure 5 is a schematic illustration of the application of a multiplex test strip device to sample solution loading in accordance with a second embodiment of the present invention. The second embodiment shown in FIG. 5 is similar to the first embodiment shown in FIG. 1, FIG. 2, FIG. 3 and FIG. 4, and the same components are denoted by the same reference numerals and will not be described herein.

This embodiment is different from the above-described first embodiment in that the test piece 10 does not have the injection hole 12 and the vent hole 14, and the test piece 10 does not have the double-stage structure 16 at the edge thereof. Further, the size of the sacrificial layer 20 is larger than the size of the test piece 10. For example, the size of the test strip 10 may be the same as that of the first embodiment described above, and the size of the main flow path 24 in the sacrificial layer 20 is, for example, greater than 22.5 mm x 22.5 mm.

Referring to FIG. 5, the sample solution can be injected from the injection hole 42 of the housing 30 to the injection flow path 22, so that the sample solution flows from the injection flow path 22 through the main flow path 24 to the end flow path 26, wherein the sample solution flows through The main flow path 24 is loaded into each of the reaction vessels 102 of the test strip 10 (the flow direction of the sample solution is as indicated by the dotted arrow in Fig. 5).

The present invention further provides an operation method of the multiplex test strip device in the first embodiment and the second embodiment described above. 6A to 6C are schematic views showing the operation method of the multiplex test strip device according to the present invention. Next, a method of operating the multiplex test strip apparatus of the present invention will be described hereinafter with reference to FIGS. 6A to 6C. It should be noted that the details of the structure of the multiplex test strip device and the loading of the sample solution have been described in detail above, and thus will not be described herein.

First, the multiplex test strip device is assembled, and the assembled multiplex test strip device is fixedly sealed by, for example, dispensing to maintain the airtight state, and the sample solution is prevented from flowing outward.

Next, the sample solution is injected into the injection flow path through the injection hole of the housing by pipetting or other suitable liquid dispenser, so that the sample solution flows from the injection flow path through the main flow path to the end flow. Road. As shown in FIG. 6A, sample solutions 70a and 70b are loaded into each reaction vessel 102 as they flow through the main flow path 24. In more detail, the total amount of the sample solution added is, for example, 60 μl.

Thereafter, oil is injected into the injection flow path through the injection hole of the casing to allow oil to flow from the injection flow path through the main flow path to the end flow path. As shown in FIGS. 6B to 6C, the oil 80 excludes the sample solution 70b not loaded in the reaction vessel 102 when flowing through the main flow path 24. In more detail, the oil is, for example, mineral oil or eucalyptus oil.

Next, during the experiment of the polymerase chain reaction, heating is performed to melt the sacrificial layer, and the melted sacrificial layer is mixed with the oil, wherein the temperature at which the sacrificial layer is heated to melt is, for example, about 60 °C. It must be noted that the test piece of the present invention is spaced apart from the sacrificial layer by a distance of at least about 10 μm (for example, 10 μm to 50 μm), and the sacrificial layer has a certain thickness (for example, a thickness of 550 μm to 590 μm). Therefore, when the melted sacrificial layer is mixed with the oil, a distance of about 600 μm between the test piece and the bottom plate can be made to allow the reaction to proceed smoothly. Since it is not necessary to add an excessive amount of sample, a certain distance can be maintained between the test piece and the bottom plate, thereby saving the amount of sample added.

In summary, the present invention provides a multiplex test strip device suitable for molecular biological detection and an operation method thereof, so that a sample solution is quickly and uniformly loaded into a test piece when flowing through a main flow path of a sacrificial layer. In a reaction vessel, the sample solution not loaded in the reaction vessel is removed by oil. In this way, all of the reaction vessels can be filled in a short period of time with a single back pipetting operation, thereby simplifying the experimental operation and saving time. In addition, since it is not necessary to add an excessive amount of sample, a certain distance can be maintained between the test piece and the bottom plate, and therefore, the present invention has an effect of saving the amount of sample added.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention, and any one of ordinary skill in the art can make some changes and refinements without departing from the spirit and scope of the present invention. The scope of the invention is defined by the scope of the appended claims.

10‧‧‧ test strips
12, 42‧‧‧ injection hole
14, 44‧‧‧ vents
16‧‧‧ double-order structure
20‧‧‧ Sacrifice layer
22‧‧‧Injection runner
24‧‧‧ main runner
26‧‧‧End runner
28‧‧‧Microchannel
30‧‧‧Shell
40‧‧‧Upper cover
50‧‧‧floor
60‧‧‧ space
70a, 70b‧‧‧ sample solution
80‧‧‧ oil
100‧‧‧Detection area
102, 160a, 160b, 160c, 160d, 160e, 160f‧‧‧Reaction containers
102a‧‧‧ Openings
102b‧‧‧ bottom
X‧‧‧first order
Y‧‧‧second order

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a block diagram showing the structure of a multiplex test strip apparatus according to a first embodiment of the present invention. Figure 2 is a schematic view showing the structure of a test piece according to a first embodiment of the present invention. Figure 3 is a cross-sectional view showing a reaction vessel of a test piece according to a first embodiment of the present invention. 4 is a schematic view showing the application of a multiplex test strip device to a sample solution according to a first embodiment of the present invention. Figure 5 is a schematic illustration of the application of a multiplex test strip device to sample solution loading in accordance with a second embodiment of the present invention. 6A to 6C are schematic views showing the operation method of the multiplex test strip device according to the present invention.

10‧‧‧ test strips

12, 42‧‧‧ injection hole

20‧‧‧ Sacrifice layer

22‧‧‧Injection runner

24‧‧‧ main runner

26‧‧‧End runner

28‧‧‧Microchannel

30‧‧‧Shell

40‧‧‧Upper cover

14, 44‧‧‧ vents

50‧‧‧floor

Claims (23)

A multiplex test strip device, comprising: a test piece having a plurality of reaction vessels, a first injection hole and a first vent hole, the reaction vessels being arranged in an array, each of the reaction vessels having an opening portion and a bottom portion; a sacrificial layer having a microchannel having intervening injection channels, a main flow path, and an end flow path, and the sacrificial layer is adapted to be assembled with the test piece, wherein the main flow path surface Assembling the opening; wherein a sample solution is injected from the first injection hole to the injection flow path to flow the sample solution from the injection flow path through the main flow path to the end flow And wherein the sample solution is loaded into each of the reaction vessels as it flows through the main flow path. The multiplex test strip device of claim 1, further comprising a housing for accommodating the test strip and the sacrificial layer, the housing having a second injection hole and a second vent hole And sequentially injecting the sample solution from the second injection hole and the first injection hole to the injection flow channel, so that the sample solution flows from the injection flow channel through the main flow path to The end flow channel, wherein the sample solution is loaded into each of the reaction vessels as it flows through the main flow path. The multiplex test strip device of claim 2, wherein the material of the housing comprises a thermally conductive material. The multiplex test strip device of claim 2, wherein the housing comprises an upper cover and a bottom plate, the upper cover is adapted to be assembled with the bottom plate, and the upper cover has a cover for receiving the test piece And a groove of the sacrificial layer, wherein the second injection hole and the second exhaust hole are located in the upper cover. The multiplex test strip device of claim 2, wherein the housing comprises an identification label. The multiplex test strip device of claim 1, wherein the material of the test strip comprises a light transmissive material. The multiplex test strip device of claim 6, wherein the light transmissive material comprises polycarbonate or polymethyl methacrylate. The multiplex test strip device of claim 1, wherein the material of the sacrificial layer comprises a wax. A multiplex test strip device comprising: a test strip having a plurality of reaction vessels, the reaction vessels being arranged in an array, each of the reaction vessels having an opening and a bottom; and a sacrificial layer having a microchannel, the micro The flow passage has injection passages, main flow passages, and end flow passages that communicate with each other, and the sacrificial layer is adapted to be assembled with the test piece, wherein the main flow passage is assembled facing the opening portion; wherein the sample is a solution is injected into the injection flow path to flow the sample solution from the injection flow path through the main flow path to the end flow path, wherein the sample solution flows through the main flow path, Loaded into each of the reaction vessels. The multiplex test strip device of claim 9, further comprising a housing for accommodating the test strip and the sacrificial layer, the housing having an injection hole and a vent hole, wherein Injecting the sample solution into the injection flow path to flow the sample solution from the injection flow path through the main flow path to the end flow path, wherein the sample solution is flowing through When the main flow path is described, it is loaded into each of the reaction vessels. The multiplex test strip device of claim 10, wherein the material of the housing comprises a thermally conductive material. The multiplex test strip device of claim 10, wherein the housing comprises an upper cover and a bottom plate, the upper cover is adapted to be assembled with the bottom plate, and the upper cover has a receiving piece for receiving the test piece And a groove of the sacrificial layer, wherein the injection hole and the vent hole are located in the upper cover. The multiplex test strip device of claim 10, wherein the housing comprises an indicia label. The multiplex test strip device of claim 9, wherein the material of the test strip comprises a light transmissive material. The multiplex test strip device of claim 14, wherein the light transmissive material comprises polycarbonate or polymethyl methacrylate. The multiplex test strip device of claim 9, wherein the material of the sacrificial layer comprises a wax. A method for operating a multiplex test strip device, comprising: assembling a multiplex test strip device, the multiplex test strip device comprising a test strip, a sacrificial layer, and a housing for accommodating the test strip and the sacrificial layer, The test piece has a plurality of reaction vessels arranged in an array, each of the reaction vessels having an opening portion and a bottom portion, the sacrificial layer having a micro flow channel having an injection flow channel and a main flow communicating with each other a channel and a terminal flow path, wherein the main flow path is assembled facing the opening; and a sample solution is injected into the injection flow path through an injection hole of the housing to allow the sample solution to be injected from the sample a flow path flows through the main flow path to the end flow path, wherein the sample solution is loaded into each of the reaction vessels as it flows through the main flow path; injection of oil through the housing a hole is injected into the injection flow path to cause the oil to flow from the injection flow path through the main flow path to the end flow path, wherein the oil is excluded from flowing through the main flow path The sample solution loaded in the reaction vessel; and heating The sacrificial layer is melted, and the melted sacrificial layer is mixed with the oil. The method of operating a multiplex test strip apparatus according to claim 17, wherein the oil comprises mineral oil or eucalyptus oil. The method of operating a multiplex test strip device of claim 17, wherein the material of the housing comprises a thermally conductive material. The method of operating a multiplex test strip device of claim 17, wherein the housing includes an indicia label. The method of operating a multiplex test strip device according to claim 17, wherein the material of the test strip comprises a light transmissive material. The method of operating a multiplex panel device according to claim 21, wherein the light transmissive material comprises polycarbonate or polymethyl methacrylate. The method of operating a multiplex panel device according to claim 17, wherein the material of the sacrificial layer comprises a wax.