KR20180125092A - Method of manufacturing microorganism immobilized high molecule media for wastewater treatment with high strength nitrogen - Google Patents

Abstract

An objective of the present invention is to provide a method of manufacturing a microorganism immobilized high molecule medium for wastewater treatment of high concentration nitrogen-containing wastewater, which manufactures the medium so that microorganisms can be attached at high concentrations, and thus the microorganism immobilized high molecule medium can treat high-concentration nitrogen even if the high-concentration nitrogen-containing wastewater flows in due to rainfall or inflow of nonpoint pollution sources, it can treat high concentration nitrogen, and can reuse the effluent. Specifically, another objective of the present invention is to provide a method of manufacturing a microorganism immobilized high molecule medium for wastewater treatment of high concentration nitrogen-containing wastewater, which is configured to select and fix at least one among nitrifying bacteria and denitrifying bacteria as a microorganism immobilized on a medium, so that the medium can effectively remove nitrification, denitrification and organic matter according to the condition of the wastewater.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a method of manufacturing a microorganism-immobilized polymer carrier for treatment of a high-concentration nitrogen-containing wastewater. BACKGROUND ART < RTI ID = 0.0 >

The present invention relates to a method for producing a microorganism-immobilized polymer carrier for treatment with a high concentration of nitrogen and wastewater, comprising the steps of preparing a carrier capable of maintaining a high concentration of a microorganism and containing a high concentration of nitrogen generated by influx of rainfall or non- It is also possible to treat wastewater.

In Korea, waste wastewater collected from point sources such as domestic wastewater, agricultural wastewater, industrial wastewater, and non-point pollution sources is treated by chemical treatment and physical filtration method. Recently, microorganisms using microorganism- A wastewater treatment technique using a wastewater treatment method is used.

Here, the carrier is intended to provide an attachment surface so that microorganisms can multiply and grow well in the bioreactor, and also has the purpose of increasing the contact effect between the substrate to be treated and the microorganism to increase the treatment effect. These carriers are manufactured and used in various ways as in Patent Documents 1 to 3 described below.

(Patent Document 1) Korean Patent No. 1344801

Wherein the sludge containing the nitrifying bacteria is immobilized by the inclusion immobilization support, wherein the sludge is added with activated sludge, water sludge, an inorganic flocculating agent and an inorganic substance, and the VSS / SS is in the range of 0.1 to 0.3, the SS concentration of the sludge in the inclusion immobilization support is not less than 2 mass%, the average particle diameter of the sludge is in the range of 10 to 30 m, the VSS concentration of the sludge in the inclusion immobilization support is To 0.2% by mass to 2% by mass.

(Patent Document 2) Korean Patent No. 1492833

The present invention relates to a carrier for purification of wastewater and a method for producing the same, which comprises preparing 30 to 80 wt% of sewage sludge, 10 to 60 wt% of glass beads and 0.5 to 10 wt% of a blowing agent and immersing the waste sludge in a liquid phase, The recyclability is increased, the environmental pollution is reduced, the adhesion of microorganisms is increased, and the purification ability of wastewater can be further improved.

(Patent Document 3) Korean Patent Publication No. 10-2017-0016126

The present invention relates to a microorganism carrier for treating wastewater and a method for manufacturing the microorganism, which can increase the specific surface area for microorganisms and improve the surface adhesion of microorganisms, A hollow hollow body which is press-molded; A microbial attached rib formed radially on the inside and outside of the hollow body and having a scratch formed on the end thereof; An inner coupling hook formed toward the center from the inside of the hollow body; An inner engaging rib formed on an inner side of the hollow body in a direction opposite to the inner engaging hook and engaged with the inner engaging hook when the hollow body is thermoformed into an " 8 " An outer engaging rib formed on the outer side of the hollow body; And an outer engaging hook formed on an outer side of the hollow body, which is a position symmetrical to the outer engaging rib, to engage with an outer engaging rib formed in another hollow body adjacent thereto.

However, these conventional carriers have difficulties in fixing microorganisms, and there is a limitation in the treatment of waste water and wastewater containing high concentration of nitrogen and the like. In addition, existing biological treatment methods used in domestic wastewater treatment plants have a limitation in lowering the concentration of effluent, and further treatment is required to treat the effluent of the effluent by treating the wastewater.

Korean Registered Patent No. 1344801 (Registered on Dec. 18, 2013) Korean Registered Patent No. 1492833 (registered on Feb. 2015) Korean Patent Laid-Open No. 10-2017-0016126 (Publication Date: 2017.02.13)

The present invention takes this point into consideration and can produce a carrier capable of adhering microorganisms at a high concentration, so that even when a lower concentration of nitrogen-containing wastewater flows into the reactor due to the inflow of rainfall or a non- It is an object of the present invention to provide a method of manufacturing a microorganism-immobilized polymer carrier for treatment of wastewater containing nitrogen at a high concentration so that effluent can be reused.

Particularly, the present invention provides a microorganism immobilized on a carrier, which can selectively fix at least one of nitrifying bacteria and denitrifying bacteria, so that it is possible to effectively remove nitrification, denitrification and organic matter according to the condition of the wastewater, It is another object of the present invention to provide a method for producing a microorganism-immobilized polymer carrier for treatment of wastewater containing wastewater.

In order to accomplish the above object, the present invention provides a method for preparing a microorganism-immobilized polymer carrier for treatment of high-concentration nitrogen-containing wastewater by treating polyvinyl alcohol (PVA) at a weight ratio of 100 to 100 wt% glycol in a ratio of 5 to 10 by weight; A second step of sterilizing and heating the mixed mixture with a high-temperature high-pressure sterilizer at a temperature of 115 to 120 DEG C for 10 to 15 minutes; A third step of cooling the mixture which has been sterilized by heating to 35 to 45 DEG C, and then adding and mixing at least one microorganism from among nitrifying bacteria and denitrifying bacteria; A fourth step of preparing the microorganism-immobilized polymer carrier by putting the mixture of the third step into a mold; And the microorganism-immobilized polymer carrier is dried at 35 to 45 ° C. for 3 to 4 hours, then boric acid at a saturation concentration (based on 20 ° C.) and calcium chloride having a concentration of 1 to 2 g / v% And a fifth step of cross-linking with time.

Particularly, the mold is characterized by a depth of 0.5 to 1.5 mm and a diameter of 5 to 15 mm.

Finally, the nitrifying bacteria are Nitrobacter sp. And Nitrosomonas europaea. And the denitrifying bacteria are selected from the group consisting of Bacillus subtilis., Paracoccus denitrificans., And Pseudomonas sp. Or the like.

The method for producing a microorganism-immobilized polymer carrier for treatment of a high concentration nitrogen-containing wastewater according to the present invention has the following effects.

(1) Nitrification and denitrification efficiency can be enhanced by immobilizing specific microorganisms.

(2) Especially, since the carrier is made into a molding frame and the wastewater is made to a size suitable for the advanced treatment process, the efficiency of the wastewater treatment can be increased.

(3) In addition, PVA (Polyvinyl alcohol) and PEG (Polyethylene glycol), which are non-toxic or biodegradable, are produced, and therefore, they are not harmful to the human body and are easily degraded.

(4) Thus, it is possible to treat waste water and wastewater containing a high concentration of nitrogen, thereby improving water environment and general living environment through purification of water quality.

FIG. 1 is a photograph of a microorganism-immobilized polymer carrier according to the present invention observed with a scanning electron microscope, wherein (a) shows Example 1, (b) shows Example 2, And Example 3, respectively.
FIG. 2 is a graph showing the total nitrogen (TN) removal rate of a microorganism-immobilized polymer carrier according to the present invention, wherein (a) shows Example 1, (b) shows Example 2, Respectively.
FIG. 3 is a graph showing the TN removal rate according to temperature change with a microorganism-immobilized polymer carrier according to [Example 2] of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Prior to this, terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms, and the inventor should properly define the concept of the term to describe its invention in the best possible way The present invention should be construed in accordance with the spirit and scope of the present invention.

Therefore, the embodiments described in the present specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention. Thus, various equivalents And variations may be present.

(Manufacturing method)

The method for producing a microorganism-immobilized polymer carrier for treatment of waste water with high concentration of nitrogen according to the present invention comprises the following five steps, and each step will be described in detail below.

The first step is mixing PVA (Polyvinyl Alcohol) and PEG (Polyethylene glycol) in distilled water. In particular, PVA and PEG have long been known to be highly stable and biodegradable and non-toxic. At this time, the PVA and PEG are mixed with distilled water at 50 to 70 ° C, most preferably 60 ° C, and they are mixed at a ratio of PVA 15 to 20 weight ratio and PEG 5 to 10 weight ratio to 100 weight ratio of distilled water. This mixing ratio is intended to increase the total nitrogen (T-N) removal rate.

The second step is a step of sterilizing and heating the mixture mixed in the first step. This is to remove microorganisms that may have been mixed with PVA, PEG, and the like, and it is performed in a temperature range where sterilization can be performed depending on the type of the microorganism. In the present invention, sterilization is performed using a high-temperature high-pressure sterilizer at 115 to 120 ° C for 10 to 15 minutes.

The third step is a step of cooling the sterilized mixture to a predetermined temperature in the second step, and then introducing the microorganism. At this time, since the sterilized mixture is usually activated at a temperature lower than the sterilization temperature, the microorganisms to be fixed are cooled to a temperature suitable for immobilization, and then the microorganisms to be fixed are added and mixed.

The microorganisms are fed at least one of nitrifying bacteria and denitrifying bacteria, wherein the nitrifying bacteria are Nitrobacter sp. And Nitrosomonas europaea. , Denitrifying bacteria such as Bacillus subtilis., Paracoccus denitrificans., And Pseudomonas sp. May be used.

In this case, R2A medium was used for Nitrobacter sp. And 0.5g / L of Yeast Extract, 0.5g / L of Casamino Acids, 0.5g / L of Proteose Peptide No. 3, 0.5g / L of Soluble Starch, 0.3g of Dipotassium Phosphate / L, 0.3 g / L of magnesium sulfate, 0.3 g / L of sodium pyruvate and 0.5 g / L of Dextrose. Nitrosomonas europaea medium was used for Medium Nitrosomonas europaea media and 2.5 g of (NH4) 2SO4, 0.5g of KH2PO4, 11.92g of HEPES, 0.5g of NaHCO3, 100mg of MgSO4, 25.0mg of CaCl2, 75mg of Fe-EDTA, Distilled water, and cultured.

Bacillus subtilis was cultured in Nutrient medium, and 3.0 g of Beef extract, 5.0 g of Peptone, 15.0 g of Agar and 1.0 L of distilled water were used as culture medium. The medium was used as a medium for Paracoccus denitrificans. 4.0 g of Peptone, 2.0 g of Yeast extract, 10.0 g of K2HPO4, 900.0 ml of distilled water, and 100.0 ml of 10% (w / v) Dextrose. Pseudomonas sp. Was cultured in YM broth medium. Yeast extract 3.0 g, malt extract 3.0 g, peptone 5.0 g, Dextrose 10.0 g, and distilled water 1.0 L were used as medium components.

Particularly, in a preferred embodiment of the present invention, the nitrifying bacteria and denitrifying bacteria should be immobilized without being killed when they are mixed into the mixture, so it is preferable to add the nitrifying bacteria to the mixture at an appropriate temperature, preferably 35 to 45 ° C.

The fourth step is a step of preparing a microorganism-immobilized polymer carrier by mixing nitrifying bacteria and denitrifying bacteria. In this step, the mixture is formed by pouring into a mold.

In a preferred embodiment of the present invention, the mold may be any size suitable for producing a microorganism-immobilized polymeric carrier. Preferably, the mold is formed to have a depth of 0.5 to 1.5 mm and a diameter of 5 to 15 mm . Most preferably, the diameter is 10 mm and the depth is 1 mm.

The fifth step is a step of drying and cross-linking the microorganism-immobilized polymer carrier. At this time, the drying is performed at 35 to 45 ° C for 3 to 4 hours. The crosslinking agent used for crosslinking after drying is crosslinked for 1 to 2 hours by using boric acid having a saturation concentration (based on 20 ° C) and calcium chloride having a concentration of 1 to 2 g / v%.

An embodiment of the microorganism-immobilized polymer carrier according to the present invention is as follows.

In a preferred embodiment of the present invention, PVA and PEG were mixed at a mixing ratio of 100 g per 100 g of distilled water as shown in the following Table 1, and then immobilized carriers were prepared. Experiments were conducted on porosity, total nitrogen (TN) Respectively.

division

Example 1
Example 2
Example 3
PVA (g)

10
15
20
PEG (g)

6
10
10

As a result, they were observed with a scanning electron microscope. As a result, the porosity of each Example was found to be as shown in [Fig. 1], in the order of [Example 2]> [Example 1]> [Example 3] As shown in Fig.

As shown in FIG. 2, the total nitrogen (TN) removal rate is lowered in all the examples as time elapses as shown in FIG. 2, . In FIG. 2, the horizontal axis X represents the experiment time (hr) and the vertical axis Y represents the total nitrogen concentration (T-N concetration [mg / L]) on the left side of each graph.

The temperature condition when the microorganism is added to the mixture of PVA and PEG is shown in the following [Table 2]. That is, all of the mixed solutions are hardened at 30 ° C in [Examples 1] to [3], and the mixed solution is not hardened at 50 ° C, but the microorganism inhibition phenomenon occurs at 60 ° C, And the mixed solution was not hardened at 40 < 0 > C, and the microorganisms could be activated.

division

30 ℃
40 ℃
50 ℃
60 ° C
Example 1
Mixed solution stiffness
Not hardened
Microbial activity possible

Microorganism Inhibition
Microbial death
Example 2
Mixed solution stiffness
Not hardened
Microbial activity possible

Microorganism Inhibition
Microbial death
Example 3
Mixed solution stiffness
Not hardened
Microbial activity possible

Microorganism Inhibition
Microbial death

The removal efficiency of T-N was evaluated by the microorganism-immobilized coating prepared by loading the microorganisms at 40 ° C, 50 ° C, and 60 ° C at which the mixed solution did not harden, and showed the best removal efficiency at 40 ° C. FIG. 3 is a graph showing TN removal rates according to temperature changes when preparing a microorganism-immobilized polymer carrier according to [Example 2] of the present invention, wherein a horizontal axis X represents an experiment time [hr] (Y) represents the total nitrogen concentration (TN concetration [mg / L]).

Claims (3)

A first step of mixing 15 to 20 weight parts of PVA (polyvinyl alcohol) and 5 to 10 weight parts of PEG (polyethylene glycol) in 100 parts by weight of distilled water at 50 to 70 DEG C;
A second step of sterilizing and heating the mixed mixture with a high-temperature high-pressure sterilizer at a temperature of 115 to 120 DEG C for 10 to 15 minutes;
A third step of cooling the mixture which has been sterilized by heating to 35 to 45 DEG C, and then adding and mixing at least one microorganism from among nitrifying bacteria and denitrifying bacteria;
A fourth step of preparing the microorganism-immobilized polymer carrier by putting the mixture of the third step into a mold; And
The microorganism-immobilized polymer carrier is dried at 35 to 45 ° C for 3 to 4 hours, then boric acid (saturated at 20 ° C) and calcium chloride at a concentration of 1 to 2 g / v% Wherein the microorganism-immobilized polymer carrier is prepared by a method comprising the steps of:
The method of claim 1,
Wherein the forming die comprises:
Wherein the microorganism-immobilized polymer carrier has a depth of 0.5 to 1.5 mm and a diameter of 5 to 15 mm.
The method of claim 1,
The nitrifying bacteria are Nitrobacter sp. And Nitrosomonas europaea. At least one of them is used,
The denitrifying bacteria include Bacillus subtilis., Paracoccus denitrificans., And Pseudomonas sp. Wherein the microorganism-immobilized polymer carrier is treated with at least one selected from the group consisting of high-concentration nitrogen-containing waste wastewater.

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