KR20180123040A - Method of evaluating the degree of graininess and method of producing dyeing liquid - Google Patents

Abstract

Provides a method for evaluating the degree of stability of safe and safe handling of the dyeing liquid. A method for evaluating the degree of graininess of grains includes a dyeing step of dyeing the grain by using a dyeing solution, an irradiating step of irradiating light to the grain, and a step of detecting the amount of light of at least one of the reflected light and the transmitted light from the light- An arithmetic step of calculating the degree of curvature of the grain based on the amount of light detected in the light amount detecting step, and a step of calculating the concentration of methylene blue and eosin so that the dissolution parameter SP is in the range of 13 to 18 And a dyeing liquid preparing step of preparing a dyeing solution by mixing a solvent other than methanol, which has undergone the adjustment.

Description

Method of evaluating the degree of graininess and method of producing dyeing liquid

The present invention relates to a method for evaluating the degree of graininess of grain and a method for producing a dyeing solution.

Conventionally, it has been known that when the outer surface of the curled paperm is removed to make the papermaking flakes, digestion and absorption of food are improved. In the case of the rice grain, rice bran is removed by feeding the raw brown rice as a raw material to a rice gruel, and processed into a rice gruel. Then, the yield when the brown rice is processed into the pure rice, that is, the weight ratio of the finished rice to the brown rice is displayed on the product. This yield is also correlated with the degree of whiteness. That is, the weight of the rice grains is reduced as the rice ball proceeds, while the weight of the rice bran decreases as the rice bran layer is removed (hereinafter referred to as the yield ratio, the degree of rice bran, the degree of whitening) (Hereinafter referred to as the "degree of whiteness"), the degree of whiteness (hereinafter referred to as "degree of whiteness") of the color of the surface of the rice is increased as the rice bran layer is removed from the brown rice .

The degree of the whiteness when the brown rice is processed into the whitening can be evaluated by these degrees of whiteness and whiteness. However, there are cases where the degree of whiteness is different or the degree of whiteness is different even if the same degree of whiteness or whiteness is the same. This tea is thought to be attributed to the degree of slacking and the degree of exfoliation of the rice bran layer.

As a method for evaluating the degree of detachment of the rice bran layer of brown rice, there is known a method of chemically evaluating the method of staining rice grains with New MG reagent (hereinafter referred to as "NMG staining solution"), Has come. This reagent dyes the perianth skin, the subcutaneous skin to green, the phyllotaxy to blue, and the endosperm to pink.

However, in this evaluation method, since a subtle difference in the degree of peeling of the rice bran layer is based on human eyes, accurate evaluation can not necessarily be obtained.

Therefore, a method of determining the processing accuracy of the rice ball described in Patent Document 1 has been developed. (B) a feeding step of supplying the rice ball to the measuring unit; (C) an irradiating step of irradiating the rice ball with light in the measuring unit; and (D) a light quantity detecting step of detecting at least one light quantity among the reflected light and the transmitted light from the irradiated rice grain; and (E) an arithmetic step of calculating the degree of graininess of the rice based on the light quantity detected in the light quantity detecting step Respectively.

This method has a dyeing step of dyeing rice grains using an NMG stain solution and a light quantity detecting step so that irrespective of the characteristics of the surface of the grain of rice or the color of the rice grain, Therefore, the grain of rice is colored. Therefore, accurate determination of the grain size of rice can be made by a single light amount measurement.

However, the Patent Document 1 has the following problems.

(1) The NMG staining solution used in the staining step is highly dangerous and poisonous to the user and requires serious handling. That is, since methanol is used as the organic solvent for the dyeing solution, it is necessary to pay attention to the flammable gas and electric spark in the storage place and the use place with high flammability.

(2) In the dyeing step, it is necessary to wash the rice grain before dyeing with water. However, if it is washed with water, the NMG dyeing solution is infiltrated into the inside, and uneven dyeing is caused or the rice bran layer is easily peeled off.

(3) Since the degree of dyeing on the grain of rice is visually confirmed in the dyeing step, individual differences arise in the evaluation of the degree of dyeing by the inspector.

Japanese Patent Application Laid-Open No. 4-95756

In view of the problems of the prior art, it is preferable that the handling of the staining solution can be performed safely and safely. In addition, it is preferable that the surface of the grain of rice be entirely stained without unevenness. Furthermore, it is preferable to provide a method for evaluating the degree of graininess of rice grain in which no individual difference occurs even in the evaluation of the degree of graininess.

The present invention has been made in order to solve at least a part of the above-mentioned problems, and can be realized, for example, in the following form.

According to a first aspect of the present invention, there is provided a method for evaluating the degree of graininess of a grain. The method for evaluating the degree of curvature of a curl includes a dyeing step of dyeing a curl using a dyeing solution, an irradiating step of irradiating light to the curl, and an amount of light of at least one of reflected light and transmitted light from the light- An arithmetic step of calculating the degree of curvature of the grain based on the amount of light detected in the light amount detecting step and a step of calculating the degree of melting of the coloring matter containing methylene blue and eosin in such a manner that the dissolution parameter SP is in the range of 13 to 18 And a dyeing liquid preparing step for preparing a dyeing solution by mixing a solvent other than methanol obtained by adjusting the concentration.

According to the first embodiment, a coloring solution is prepared by mixing a solvent (except for methanol) whose concentration has been adjusted so that the dissolution parameter SP is in the range of 13 to 18, to the dye containing methylene blue and eosin. That is, in preparing the dyeing solution, a solvent whose concentration is adjusted so that the solubility parameter SP is in the range of 13 to 18 is used instead of the organic solvent used in the prior art. Therefore, the lethal dose of the staining liquid is reduced compared to the conventional technique, so that even when the staining liquid is handled, intentionally ingested, and miscooked during handling, it is safe and safe compared with the prior art. Therefore, handling of the dyeing solution is easy and the risk is reduced.

According to the second aspect of the present invention, in the first aspect, the graininess degree evaluation method is performed after the dyeing step using a solution other than methanol, the concentration of which is adjusted so that the dissolution parameter SP is in the range of 13 to 18 Before the irradiation step, a cleaning step for cleaning the dyed dyed dyed with the dyeing solution is provided.

According to the second embodiment, the dyed grains are washed using a concentrated solution (except for methanol) so that the solubility parameter SP value is in the range of 13 to 18, preferably in the range of 14 to 16 . Therefore, as compared with the case of washing with water, the dyeing solution is not introduced into the center portion of the curled grains, and the dyeing is performed uniformly throughout the curled grain. For this reason, the degree of staining of the grainy grain can be easily confirmed with eyes, and individual differences are hard to occur in the evaluation.

According to the third aspect of the present invention, in the first or second aspect, the composition of the dyeing solution produced in the dyeing step is 0.1% methylene blue, 0.1% eosin, 99.8% aqueous ethanol solution .

According to a fourth aspect of the present invention, in the first or second aspect, the composition of the dyeing solution produced in the dyeing step is 0.1% methylene blue, 0.1% eosine, 99.8% isopropanol aqueous solution .

According to a fifth aspect of the present invention, in any one of the first to fourth aspects, the solvent is an aqueous ethanol solution, and the concentration of the aqueous ethanol solution is 80%. According to a sixth aspect of the present invention, in any one of the third to fifth aspects including the second or the second aspect, the solution is an aqueous ethanol solution, and the concentration of the aqueous ethanol solution is 80%.

According to a seventh aspect of the present invention, in any one of the first to fourth aspects, the solvent is an isopropanol aqueous solution, and the concentration of the isopropanol aqueous solution is 72.5%. According to the eighth aspect of the present invention, in any one of the third to fifth aspects including the second or the second aspect, the solution is an isopropanol aqueous solution, and the concentration of the isopropanol aqueous solution is 72.5%.

According to the eighth aspect of the present invention, there is provided a method for producing a dyeing liquid for dyeing a grain of a grain in order to evaluate the graininess of cereals. The method for producing the dyeing solution includes a step of mixing a solvent other than methanol in which the concentration of the dye containing methylene blue and eosin is adjusted so that the solubility parameter SP is in the range of 13 to 18 to obtain a dyeing solution do. According to the eighth aspect, the same effect as the first aspect is exhibited. It is possible to add any of the second to seventh modes to the eighth mode.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic explanatory view showing a configuration of a rice grain discriminating apparatus for carrying out a roughness evaluation method according to an embodiment of the present invention. FIG.
2 is a flow chart for carrying out a method for evaluating the degree of graininess of rice grains according to an embodiment of the present invention.
Fig. 3 is a measurement flow chart for carrying out the method for evaluating the degree of graininess of rice flour.
4 is an example of the calculation result of the evaluation of the degree of graininess of the rice grain.
FIG. 5 is a flow chart for carrying out a conventional method for evaluating the degree of graininess of rice grains.

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments for carrying out the present invention will be described with reference to the drawings. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic explanatory view showing a configuration of a rice grain discriminating apparatus for carrying out a turbidity evaluation method according to an embodiment of the present invention. FIG. Reference numeral 1 denotes a mechanism used in a dyeing step (S3) for dyeing rice grains using a staining liquid. Reference numeral 2 denotes an image pickup device for observing the color of the rice grain after dyeing (to be used in the irradiation step (S7) and the light amount detection step (S8) to be described later). Reference numeral 3 denotes a discriminating device for calculating the degree of corn graininess (used in the corrugation operation step (S9)) based on an image picked up by the image pickup device 2. [

The instrument 1 used in the dyeing process comprises a test tube 4, a test tube 5 supported by the test tube 4, an absorbing filter paper 6, And a pipette (not shown) may be provided for injecting the liquid. As the image pickup device 2, for example, a commercially available scanner for converting a source of characters, photographs, pictures, etc. into digital image data can be used. As the discriminating device 3, for example, a portable tablet terminal or a notebook type general purpose personal computer can be employed. The image pickup device 2 and the discrimination device 3 are connected so as to be capable of data communication via a cable 7. [

The image capturing apparatus 2 generally includes a main body 8, an image reading section 9 having glass or the like disposed on the upper surface of the main body 8, And an opening / closing cover (10) for covering the portion (9). Inside the main body 8, there is provided a light-receiving unit for receiving light from at least one of a light source for emitting light toward the image reading unit 9 and reflected light and transmitted light from a subject to be imaged ). The light source may be, for example, a white fluorescent lamp or a white LED. The light receiving unit may be, for example, a color CCD line sensor or the like.

A picked up image (such as a piece of raw material such as rice grain) which is easily rolled and overflows in the image reading section 9 is received on a specimen tray 11 which is transparent only on the bottom surface, (11) is loaded on the image reading section (9). This facilitates handling of the image pickup object. In addition, since the image reading section 9 need not be cleaned every time it is measured, convenience is improved. The sample tray 11 has a transparent bottom surface portion 11a, rising walls 11b to 11e surrounding four sides of the bottom surface portion 11a and a pair of knobs 11f and 11f. The rice grains contained in the sample tray 11 need not be aligned strictly and strictly but may be accommodated in a dispersed state such that the grains of the rice grains do not overlap each other. This is because the image obtained by the light-receiving unit through the image reading unit 9 can then align the plurality of pieces of rice grains neatly by the image processing in the discriminating apparatus 3 thereafter.

The discrimination device 3 is provided with a main body part 12 and a display part 13. The main body unit 12 includes an image processing unit, a feature extraction unit, and a comparison calculation unit. These functions are realized by the CPU executing a predetermined program installed in the main body 12. [ The image processing section performs image processing of image pickup data of the rice grain picked up by the image pickup device 2. [ The feature extracting unit extracts optical information related to the color of the grain of rice, shape information relating to the outer shape, and the like. The comparison operation unit compares each piece of information extracted in the feature extraction unit with a threshold or the like stored in advance in order to discriminate the quality. The display unit 13 displays the result obtained in the comparison operation unit.

Further, the apparatus for discriminating the grain of rice is not limited to the scanning method described above. For example, it may be a system for obtaining an image while descending one grain of rice as disclosed in Japanese Patent Laid-Open No. 2008-298695. In the present embodiment, a scanning type grain scanner (manufactured by Satsuma Kagaku Co., Ltd., format: RSQI10A) as shown in Fig. 1 was used.

Hereinafter, a method for evaluating the degree of graininess of rice grains according to an embodiment of the present invention using the apparatus having the above-described structure will be described with reference to FIG.

2 is a flow chart for carrying out a method for evaluating the degree of graininess of rice grains according to an embodiment of the present invention. 5 is a flowchart for performing the conventional method for evaluating the degree of graininess of rice grains, which will be described in comparison with FIG.

≪ Preparation of raw material for NMG (dyeing liquid preparation) step (S1) >

Step S1 is a step of preparing a NMG stock solution by adding a safe and safe solvent replacing methanol to a dye containing methylene blue and eosin. As a substitute for safe and safe methanol, the present inventors have intensively studied and found that the dissolution parameter (SP value) used as a criterion of the solubility of a two-component system solution. The dissolution parameter (SP value) of methanol is known to be 14.5 to 14.8. By using this value as an index, a safe and safe solvent is produced, and an alternative solvent becomes usable. As an alternative solvent, a solvent (except for methanol) whose concentration adjustment has been carried out so that the solubility parameter SP value is in the range of 13 to 18, preferably in the range of 14 to 16 may be used.

For example, in the case of using ethanol for preparing a safe and safe solvent, when 80% of ethanol and 20% of water are mixed, the dissolution parameter (SP value) is 14.8. In addition, when isopropyl alcohol (IPA) was used in preparing a safe and safe solvent, the dissolution parameter (SP value) was 14.8 when 72.5% IPA and 27.5% water were mixed. When these were used as a solvent for the dye containing methylene blue and eosin, it became a color matching standard almost identical to methanol. On the other hand, in step S10 of FIG. 4, it can be seen that the conventional method for producing an NMG stock solution is that a stock solution of methanol is added as a solvent to a dye containing methylene blue and eosin.

≪ NMG original solution dilution (dyeing solution preparation) step (S2) >

Step S2 (diluting step S2) is a step of diluting the NMG stock solution prepared in step S1 to generate a staining solution. Since the concentration of the NMG stock solution is too high, a dyeing solution may be prepared as a diluting solution using a solvent at the time of preparing the undiluted solution. The mixing ratio may be, for example, a stock solution: diluted solution = 1: 3. On the other hand, step S11 of FIG. 4 is a conventional dilution step of the NMG stock solution, and it can be seen that the stock solution of NMG is diluted with the diluted methanol solution. It can be seen that handling of the diluted solution becomes more dangerous than that of this embodiment because the methanol stock solution is used as the diluting solution.

≪ Dyeing process (S3) >

Step S3 is a step of adding the dyeing solution prepared in step S2 to the rice grain and dyeing. In the test tube 5 shown in Fig. 1, 5 g of pure white rice was put in advance. 5 ml of the dyeing solution prepared in step S2 is added to the test tube 5 and the rice ball is dyed by shaking for 1 to 2 minutes. On the other hand, steps S12 and S13 in FIG. 4 are steps of adding a conventional dyeing solution to rice flour and dyeing it. Since step S12 for washing rice flour is set as the previous step of dyeing step S13, rice bran powder in the form of flour is removed or the dyeing liquid is easily impregnated into the rice flour. On the other hand, according to this step S12 (washing step), the dyeing solution is infiltrated into the inside of the rice grain, and uneven dyeing is caused or rice bran is easily peeled off. Therefore, it is preferable to omit this step S12.

≪ Rice surface washing step (S4) >

Step S4 is a step of discarding the dyeing solution used in step S3 and cleaning the surface of the rice grain. When the surface of the rice ball is washed, it may be washed 2 to 3 times using the diluting liquid used in the diluting step S2. As a result, the dyeing solution does not penetrate into the center of the grain of rice, and dyeing is performed irregularly throughout the whole grain of the rice. On the other hand, in step S14 of cleaning the surface of the rice grain of the conventional example shown in Fig. 5, since water is used for cleaning the surface, the dyeing solution is infiltrated to the inside, and uneven dyeing is caused or rice bran is easily peeled off. Therefore, when cleaning, it is preferable to use a diluting liquid as in this embodiment.

<Air drying process (S5)>

Step S5 is a step of air-drying the rice flour washed in Step 4. [ After the washing of the rice balls, the rice balls are taken out on the filter paper 6 shown in Fig. 1 and air-blown, or an absorbing (wetting) sheet (a polymer absorbing polymer-introduced sheet or a super absorbent sheet such as an absorbent pad (Hashimoto Cross) Rice straw is taken out from the surface of the rice cloth to wipe the surface water, and thereafter, coloring determination using a rice grain discriminating device is carried out (for example, Is done. The step S15 in Fig. 5 is the same as the air drying process (step S5) in Fig.

Fig. 3 is a measurement flowchart for carrying out a method for evaluating the degree of graininess of rice using the rice grain discriminating device shown in Fig. 1. Fig.

&Lt; Feeding step (S6) of supplying the rice grain to the measuring part >

First, in step S6, the grain of rice that has undergone the dyeing and air-drying process is supplied to the measuring section of the image capturing apparatus 2. Concretely, first, on the sample tray 11, rice grains having undergone a dyeing and air drying process are arranged. Then, by holding the grips 11f and 11f by hand and waving the sample tray 11, the grain of rice on the sample tray 11 is dispersed so that the grain of the grain does not overlap each other. Thereafter, the sample tray 11 is loaded on the image reading section 9. [

&Lt; Irradiation step for irradiating light (S7) >

Then, by closing the opening / closing cover of the image pickup device 2, the measurement preparation is completed. Subsequently, in step S7, light is irradiated from the light source built in the main body 8 toward the image reading section 9. [

&Lt; Light amount detecting step (S8) >

Following the irradiation of light, at step S8, at least one light amount of the reflected light and the transmitted light from the reflected-light rice grains is detected by the light-receiving unit built in the main body 8.

&Lt; Calculation process of rice grain (S9)

Then, in step S9, the degree of graininess of the rice grain is calculated based on the amount of light detected in the light amount detecting step S8. This calculation is performed, for example, by the discrimination apparatus 3 (more specifically, the above-described image processing section, feature extraction section and comparison operation section).

The result obtained in the comparison operation unit is displayed on the display unit 13 of the determination device 3.

Fig. 4 is an example of the calculation result of the degree of corrugation evaluation of the grain of rice displayed on the display section 13 of the discriminating apparatus 3. Fig. 4A is a diagram of a color vision observation (image as it is imaged by the imaging device 2), and FIG. 4B is an image processing evaluation An image obtained by performing image processing, feature extraction, and comparison operation).

When the rice grain is stained by the dyeing operation shown in Fig. 2, the pericarp or bell skin in the brown rice becomes green, the color of the chickpea layer becomes blue, and the part of the rice bran (starch layer) of the rice cake becomes color pink. According to the eye observation evaluation (the original picked up by the image pickup device 2, see No. 1 in FIG. 4 (a)), the distribution portion (starch layer) And it is judged that the collar layer is a little color. On the other hand, according to the image evaluation (evaluation for processing the original picked up by the image pickup device 2 to perform image processing or the like, i.e., mechanical evaluation, see No.1 in FIG. 4B), the coloring state Is displayed in detail. More specifically, the occupied area occupied by the starch layer and the occupied area occupied by the horny layer are quantitatively displayed, and the presence or absence of the dorsal root bark is displayed. For example, in No. 1 of Fig. 4 (b), "starch layer is 98%, horny layer is 2%, and no abdominal cavity" is indicated. Thus, it is possible to quantitatively evaluate the degree of graininess of rice without resorting to human subjectivity. That is, it is possible to provide a method for evaluating the degree of graininess of rice grain with no individual difference and with high precision. By the same evaluation method, it is easy to understand that quantitative display indicates that "there is a stomach cavity and a horny layer is relatively high" as shown in Nos. 3 to 5 in Fig. 4 (b). Further, since it is known that the degree of grinding of the grain of rice and the degree of removal of the rice bran are also bad, the quality, particularly the degree of distribution of the grain of rice to the consumer, is lowered.

As described above, the graininess evaluation method according to one embodiment of the present invention includes a dyeing step (step S3) for dyeing the curved grain using a dyeing solution, an irradiating step (step S7) (Step S8) of detecting at least one of the reflected light and the transmitted light from the irradiated curved grains; and a calculation step (step S9) of calculating the degree of curvature based on the amount of light detected in the light amount detection step ), A dyeing liquid preparation step (steps S1 and S2) for preparing a dyeing solution by mixing a solvent other than methanol in which the concentration of methylene blue and eosin is adjusted so that the solubility parameter SP is in the range of 13 to 18, S2. Therefore, the lethal dose of the staining solution is reduced compared with the conventional technique, so that even when the staining liquid is handled, intentionally ingested, or miscooked during handling, it is safe and safe compared with the prior art. Therefore, handling of the dyeing liquid is easy and the risk is reduced.

Although the embodiments of the present invention have been described above, the embodiments of the invention described above are for the purpose of facilitating understanding of the present invention and are not intended to limit the present invention. The present invention can be modified and improved without departing from the spirit thereof, and it goes without saying that the present invention includes equivalents thereof. In addition, within the range in which at least part of the above-described problems can be solved or at least a part of the effect is exhibited, any combination or omission of each component described in the claims and specification can be made. For example, the method for evaluating the degree of graininess of the above-described rice grain can be realized as a method for evaluating the degree of graininess of any grain such as barley, corn, and soybeans. Further, the above-described method of producing a staining solution is not limited to a method of mechanically evaluating the degree of latitude based on at least one of reflected light and transmitted light from a curved surface by irradiating light to the curved surface, Method (e.g., a method for evaluating the degree of dyeing with a simple eye, etc.).

The present invention can be applied to a method for evaluating the degree of graininess of cereals such as barley, corn, and beans in addition to the grain of rice.

1 Mechanism
2 imaging device
3 discrimination device
4 Test tube stand
5 examiners
6 filter paper
7 cable
8 Body
9 image reading section
10 opening cover
11 Sample tray
12 body part
13 Display

Claims (9)

A method for evaluating graininess of a grain,
A dyeing step for dyeing the curl using a dyeing solution,
An irradiating step of irradiating the curved surface with light,
A light amount detecting step of detecting at least one light amount of reflected light and transmitted light from the curved surface irradiated with the light,
An arithmetic step of calculating a degree of incidence of the curved line based on the amount of light detected in the light amount detecting step,
A coloring liquid containing methylene blue and eosin is subjected to concentration adjustment so that the solubility parameter SP is in the range of 13 to 18 and a solvent other than methanol is mixed to prepare the dyeing solution
The method comprising the steps of: The method according to claim 1, wherein, after the dyeing step and before the step of irradiating, a solution other than methanol, the concentration of which is adjusted so that the solubility parameter SP is in the range of 13 to 18, And a cleaning step
A method of evaluating the roughness of a grain. The dyeing composition according to claim 1 or 2, wherein the composition of the dyeing solution is 0.1% methylene blue, 0.1% eosin, and 99.8% aqueous ethanol solution
A method of evaluating the roughness of a grain. The dyeing solution according to claim 1 or 2, wherein the composition of the dyeing solution is 0.1% methylene blue, 0.1% eosin, and 99.8% isopropanol aqueous solution
A method of evaluating the roughness of a grain. The method according to claim 1, wherein the solvent is an aqueous solution of ethanol, and the concentration of the aqueous ethanol solution is 80%
A method of evaluating the roughness of a grain. 3. The method according to claim 2, wherein the solution is an aqueous solution of ethanol, and the concentration of the aqueous ethanol solution is 80%
A method of evaluating the roughness of a grain. The method of claim 1, wherein the solvent is an isopropanol aqueous solution, and the concentration of the isopropanol aqueous solution is 72.5%
A method of evaluating the roughness of a grain. 3. The method according to claim 2, wherein the solution is an isopropanol aqueous solution, and the concentration of the isopropanol aqueous solution is 72.5%
A method of evaluating the roughness of a grain. A method for producing a dyeing liquid for dyeing grains so as to evaluate the degree of graininess,
And a step of mixing a solvent other than methanol in which the concentration of methylene blue and eosin is adjusted so that the solubility parameter SP is in the range of 13 to 18 to obtain the dyeing solution
A method for producing a staining solution.

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