KR20180115397A - Anti-aging Composition for Skin External Application Comprising Callus extracts from Citrus junos Siebold ex Tanaka and Pyrus pyrifolia Nakai and Xanthan Gum - Google Patents
Anti-aging Composition for Skin External Application Comprising Callus extracts from Citrus junos Siebold ex Tanaka and Pyrus pyrifolia Nakai and Xanthan Gum Download PDFInfo
- Publication number
- KR20180115397A KR20180115397A KR1020170047598A KR20170047598A KR20180115397A KR 20180115397 A KR20180115397 A KR 20180115397A KR 1020170047598 A KR1020170047598 A KR 1020170047598A KR 20170047598 A KR20170047598 A KR 20170047598A KR 20180115397 A KR20180115397 A KR 20180115397A
- Authority
- KR
- South Korea
- Prior art keywords
- extract
- callus
- culture
- xanthan gum
- composition
- Prior art date
Links
- 239000000284 extract Substances 0.000 title claims abstract description 127
- 206010020649 Hyperkeratosis Diseases 0.000 title claims abstract description 98
- 239000000203 mixture Substances 0.000 title claims abstract description 81
- 239000000230 xanthan gum Substances 0.000 title claims abstract description 69
- 229920001285 xanthan gum Polymers 0.000 title claims abstract description 69
- 229940082509 xanthan gum Drugs 0.000 title claims abstract description 69
- 235000010493 xanthan gum Nutrition 0.000 title claims abstract description 69
- 241000951471 Citrus junos Species 0.000 title description 9
- 244000088401 Pyrus pyrifolia Species 0.000 title description 2
- 235000011400 Pyrus pyrifolia Nutrition 0.000 title description 2
- 230000003712 anti-aging effect Effects 0.000 title description 2
- 240000004307 Citrus medica Species 0.000 claims abstract description 45
- 235000014443 Pyrus communis Nutrition 0.000 claims abstract description 23
- 230000037303 wrinkles Effects 0.000 claims abstract description 19
- 239000004480 active ingredient Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 49
- 239000008213 purified water Substances 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 15
- 241000207199 Citrus Species 0.000 claims description 11
- 235000020971 citrus fruits Nutrition 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 11
- 239000006228 supernatant Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- 238000010298 pulverizing process Methods 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 6
- 230000002265 prevention Effects 0.000 claims description 5
- 238000002481 ethanol extraction Methods 0.000 claims description 4
- 241000218376 Magnoliales Species 0.000 claims description 2
- 239000002537 cosmetic Substances 0.000 abstract description 32
- 230000000694 effects Effects 0.000 abstract description 15
- 102000008490 2-Oxoglutarate 5-Dioxygenase Procollagen-Lysine Human genes 0.000 abstract description 13
- 108010020504 2-Oxoglutarate 5-Dioxygenase Procollagen-Lysine Proteins 0.000 abstract description 13
- 102000004079 Prolyl Hydroxylases Human genes 0.000 abstract description 9
- 108010043005 Prolyl Hydroxylases Proteins 0.000 abstract description 9
- 239000000463 material Substances 0.000 abstract description 8
- 241000196324 Embryophyta Species 0.000 description 33
- 241000220324 Pyrus Species 0.000 description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 22
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 18
- 238000002360 preparation method Methods 0.000 description 17
- 238000004519 manufacturing process Methods 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 14
- 230000014509 gene expression Effects 0.000 description 13
- 239000002609 medium Substances 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- -1 polyoxyethylene Polymers 0.000 description 11
- 238000000605 extraction Methods 0.000 description 9
- 239000006210 lotion Substances 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 241000193830 Bacillus <bacterium> Species 0.000 description 8
- 238000009472 formulation Methods 0.000 description 8
- 108010035532 Collagen Proteins 0.000 description 7
- 102000008186 Collagen Human genes 0.000 description 7
- 229920001436 collagen Polymers 0.000 description 7
- 239000006071 cream Substances 0.000 description 6
- 210000002950 fibroblast Anatomy 0.000 description 6
- 235000011187 glycerol Nutrition 0.000 description 6
- 239000003960 organic solvent Substances 0.000 description 6
- 239000002994 raw material Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N squalane Chemical compound CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 6
- 238000003809 water extraction Methods 0.000 description 6
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 5
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 5
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 5
- 230000002421 anti-septic effect Effects 0.000 description 5
- 239000000686 essence Substances 0.000 description 5
- 239000000499 gel Substances 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 238000004161 plant tissue culture Methods 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000001804 emulsifying effect Effects 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 235000019198 oils Nutrition 0.000 description 4
- 235000013599 spices Nutrition 0.000 description 4
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 3
- GFVYDNHWTCHDSN-UHFFFAOYSA-N 14-methylpentadecyl octanoate Chemical compound CCCCCCCC(=O)OCCCCCCCCCCCCCC(C)C GFVYDNHWTCHDSN-UHFFFAOYSA-N 0.000 description 3
- RFVNOJDQRGSOEL-UHFFFAOYSA-N 2-hydroxyethyl octadecanoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCCO RFVNOJDQRGSOEL-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000000692 anti-sense effect Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 239000004359 castor oil Substances 0.000 description 3
- 235000019438 castor oil Nutrition 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 229940082500 cetostearyl alcohol Drugs 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000037319 collagen production Effects 0.000 description 3
- 239000003995 emulsifying agent Substances 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 3
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 3
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000006870 ms-medium Substances 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 description 3
- GLDOVTGHNKAZLK-UHFFFAOYSA-N octadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCCCO GLDOVTGHNKAZLK-UHFFFAOYSA-N 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 238000003757 reverse transcription PCR Methods 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 239000001587 sorbitan monostearate Substances 0.000 description 3
- 235000011076 sorbitan monostearate Nutrition 0.000 description 3
- 229940035048 sorbitan monostearate Drugs 0.000 description 3
- 229940032094 squalane Drugs 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- 210000000130 stem cell Anatomy 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- OULAJFUGPPVRBK-UHFFFAOYSA-N tetratriacontyl alcohol Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCO OULAJFUGPPVRBK-UHFFFAOYSA-N 0.000 description 3
- 239000001993 wax Substances 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 239000005595 Picloram Substances 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000003796 beauty Effects 0.000 description 2
- 229960003237 betaine Drugs 0.000 description 2
- 230000036570 collagen biosynthesis Effects 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000469 ethanolic extract Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000003906 humectant Substances 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 229940057995 liquid paraffin Drugs 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 239000012046 mixed solvent Substances 0.000 description 2
- 239000003607 modifier Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- NQQVFXUMIDALNH-UHFFFAOYSA-N picloram Chemical compound NC1=C(Cl)C(Cl)=NC(C(O)=O)=C1Cl NQQVFXUMIDALNH-UHFFFAOYSA-N 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 210000004761 scalp Anatomy 0.000 description 2
- 229920002545 silicone oil Polymers 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 239000006097 ultraviolet radiation absorber Substances 0.000 description 2
- 239000000080 wetting agent Substances 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- 229940058015 1,3-butylene glycol Drugs 0.000 description 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 1
- VOUAQYXWVJDEQY-QENPJCQMSA-N 33017-11-7 Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)NCC(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N1[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)CCC1 VOUAQYXWVJDEQY-QENPJCQMSA-N 0.000 description 1
- DEXFNLNNUZKHNO-UHFFFAOYSA-N 6-[3-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperidin-1-yl]-3-oxopropyl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C1CCN(CC1)C(CCC1=CC2=C(NC(O2)=O)C=C1)=O DEXFNLNNUZKHNO-UHFFFAOYSA-N 0.000 description 1
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 description 1
- 108010075254 C-Peptide Proteins 0.000 description 1
- 101100000858 Caenorhabditis elegans act-3 gene Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 238000011891 EIA kit Methods 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 239000001653 FEMA 3120 Substances 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000004867 Hydro-Lyases Human genes 0.000 description 1
- 108090001042 Hydro-Lyases Proteins 0.000 description 1
- HEFNNWSXXWATRW-UHFFFAOYSA-N Ibuprofen Chemical compound CC(C)CC1=CC=C(C(C)C(O)=O)C=C1 HEFNNWSXXWATRW-UHFFFAOYSA-N 0.000 description 1
- 235000019493 Macadamia oil Nutrition 0.000 description 1
- 241000218378 Magnolia Species 0.000 description 1
- PKVZBNCYEICAQP-UHFFFAOYSA-N Mecamylamine hydrochloride Chemical compound Cl.C1CC2C(C)(C)C(NC)(C)C1C2 PKVZBNCYEICAQP-UHFFFAOYSA-N 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 241001502129 Mullus Species 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 229910004616 Na2MoO4.2H2 O Inorganic materials 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 108010050808 Procollagen Proteins 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 239000004902 Softening Agent Substances 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 102000004887 Transforming Growth Factor beta Human genes 0.000 description 1
- 108090001012 Transforming Growth Factor beta Proteins 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 244000295923 Yucca aloifolia Species 0.000 description 1
- 235000004552 Yucca aloifolia Nutrition 0.000 description 1
- 235000012044 Yucca brevifolia Nutrition 0.000 description 1
- 235000017049 Yucca glauca Nutrition 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000003862 amino acid derivatives Chemical class 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000003738 black carbon Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 235000019437 butane-1,3-diol Nutrition 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- SZXQTJUDPRGNJN-UHFFFAOYSA-N dipropylene glycol Chemical compound OCCCOCCCO SZXQTJUDPRGNJN-UHFFFAOYSA-N 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000004088 foaming agent Substances 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 239000007902 hard capsule Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000012676 herbal extract Substances 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229940119170 jojoba wax Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 125000001288 lysyl group Chemical group 0.000 description 1
- 239000010469 macadamia oil Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000036244 malformation Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 239000004530 micro-emulsion Substances 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 210000005059 placental tissue Anatomy 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 1
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 1
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229960003471 retinol Drugs 0.000 description 1
- 235000020944 retinol Nutrition 0.000 description 1
- 239000011607 retinol Substances 0.000 description 1
- 238000012340 reverse transcriptase PCR Methods 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 239000003352 sequestering agent Substances 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 210000001626 skin fibroblast Anatomy 0.000 description 1
- 230000004215 skin function Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000007901 soft capsule Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000036642 wellbeing Effects 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
본 발명은 주름 개선을 위한 유효성분으로써 배 또는 유자 캘러스 배양물 또는 그 추출물, 그리고 잔탄검을 함유하는 주름개선용 피부 외용제 조성물에 관한 것이다.
본 발명에 따른 피부 외용제 조성물은, 특히 잔탄검을 주름 개선을 위한 유효성분으로써 배 또는 유자 캘러스 배양물 또는 그 추출물과 함께 함유함으로써, 콜라겐 합성에 중요한 프롤린 히드록실라제 및 라이실 히드록실라제 효소들의 발현을 증가시켜 우수한 주름 개선 효과를 가지고 있어, 기능성 화장품 소재로써 활용가치가 높다. The present invention relates to a composition for external application for wrinkles containing wrinkle-improving wrinkle-improving pear or citron callus culture product or extract thereof and xanthan gum.
The composition for external application for skin according to the present invention is characterized in that xanthan gum is contained as an active ingredient for improving wrinkles and is contained together with a bait or citron callus culture or an extract thereof so that proline hydroxylase and lysylhydroxylase enzyme And it has a wrinkle-improving effect, and thus it is highly useful as a functional cosmetic material.
Description
본 발명은 주름 개선을 위한 유효성분으로써 배 또는 유자 캘러스 배양물 또는 그 추출물, 그리고 잔탄검을 함유하는 주름개선용 피부 외용제 조성물에 관한 것이다.The present invention relates to a composition for external application for wrinkles containing wrinkle-improving wrinkle-improving pear or citron callus culture product or extract thereof and xanthan gum.
화장품 산업은 기술적 측면에서 화학, 생물학, 생리학, 약학 등의 기초과학과 응용기술이 복합적으로 적용되는 분야로, 기술집약적이고 고부가가치 산업이다. 오늘날 화장품은 사람의 피부에 직접 사용하는 제품으로써 피부에 대한 안전성, 효능, 사용 편의성등을 갖추어야 하며, 아름다움을 가꾸는 미적 기능 뿐 아니라, 피부를 보호, 청결, 보습, 유연 및 피부 생리적 기능을 유지하는 생물학적 기능 또한 갖추어야 한다. 특히 화장품 산업은 급속한 산업화가 진행되고 있고, 고령화 현상이 심화됨에 따라, 환경 및 건강에 대한 중요성이 부각되어 웰빙 문화가 현대 사회의 메가트렌드로 자리잡았다. 이러한 고도산업의 발달에 따라 환경변화에 기인한 각종 피부 질환이 증가하고 있으며, 바이오벤처 및 화장품, 생명공학 업계에서는 천연소재의 고기능성을 활용한 미백 및 항노화 연구가 활발히 진행되고 있다. The cosmetics industry is a technology-intensive, high-value-added industry in which basic science and applied technologies such as chemistry, biology, physiology, and pharmacy are applied in a technical aspect. Today, cosmetics are products that are directly used on human skin. They should have safety, efficacy and ease of use for skin. They should not only provide aesthetic functions to maintain beauty, but also protect skin, clean, moisturize, It should also have biological functions. Especially, the cosmetics industry has been rapidly industrialized and the aging phenomenon has deepened, so importance of environment and health has been emphasized, and well-being culture has become a mega trend of modern society. Various kinds of skin diseases caused by environmental changes are increasing with the development of such advanced industries, and in the bio venture, cosmetics and biotechnology industries, researches on whitening and anti-aging using high functionality of natural materials are actively being carried out.
이처럼 최첨단 생명공학 기술의 발전 및 국가경제가 성장함에 따라 화장품 산업에서도 첨단 기술이 도입되었으며, 준치료제 개념의 코스메슈티컬(화장품을 뜻하는 코스메틱과 치료제를 뜻하는 파마슈티컬의 신조 합성어) 개발이 화장품 산업의 메가트렌드로 부각되고 있다.As the development of cutting-edge biotechnology and the growth of the national economy, advanced technology has been introduced in the cosmetics industry, and the development of cosmeceuticals (a cosmetic for cosmetics and a new compound word for pharmaceuticals, It is becoming a mega trend of the industry.
특히 최근 기능성이 높은 바이오소재들이 접목되어 생물학적인 유효성이 높은 제품들을 개발하고자 하는 시도들이 화장품 업계와 바이오기업들을 중심으로 활발히 진행되고 있다. 기존 화장품 소재들은 식물이나 한방 추출물의 민간요법 또는 동의보감에 의한 정보를 바탕으로 한 전통적인 방식의 추출물이 화장품에 쓰였다. 반면 현재는 생물학적인 기반으로 유효성이 확인된 추출물이나 추출법이 적용되어 피부에 대한 기능성이 탁월하고 효과 및 안전성이 우수한 기능성 화장품 소재에 대한 니즈가 증가되고 있는 추세이다. Particularly, recent attempts to develop products with high biological efficiency by incorporating highly functional biomaterials have been actively conducted by the cosmetics industry and bio companies. Existing cosmetic materials were used in cosmetics, traditional extracts based on information from plants, oriental herbal extracts, or Donguibogam. On the other hand, there is an increasing need for functional cosmetic materials having superior skin function and excellent efficacy and safety by applying an extract or extraction method that has been validated on a biological basis.
한편, 식물캘러스 (식물줄기세포)는 식물의 분화되지 않은 부정형의 세포덩어리를 의미하는 것으로, 식물이 상처가 있을 때 생성되는 분열조직이 형성한 종양조직이 대표적이다. 식물은 크게 세포 분열을 하는 분열조직과 그렇지 않는 영구 조직으로 구성되어 있으며, 이중 처음 분열 조직의 세포를 영양 배지에 넣고 키우면 캘러스가 형성된다. 이후 부정배가 형성되면서 식물체로 분화된다. 캘러스는 식물의 각 조직으로 분회되므로 "식물의 줄기세포"라고 정의된다. 친환경 녹색성장과 매우 밀접한 관계를 가지는 식물조직 배양기술은 1990년대 이후 국내 농촌진흥청및 대학가에서 주로 병저항성, 특정 이차대사산물 축적을 목적으로 많이 활용되고 있다. 최근 식물줄기세포(캘러스) 소재는 바이오벤처기업 중심으로 활발히 이뤄지고 있다. On the other hand, a plant callus (plant stem cell) refers to an indefinite amorphous cell mass of a plant, and is a typical tumor tissue formed by a cleavage tissue formed when a plant is wounded. Plants are composed of dividing tissues that are largely dividing cells and permanent tissues that are not. Among them, callus is formed when cells of the first dividing tissue are put into a nutrient medium. It is then differentiated into plants with the formation of malformations. Callus is defined as "plant stem cells" because it is divided into each tissue of the plant. Plant tissue culture technology, which has a close relationship with environmentally friendly green growth, has been widely used for the purpose of accumulating disease resistance and specific secondary metabolites mainly in the domestic RDA and the university since the 1990s. Recently, plant stem cell (callus) material is actively being centered on bio-venture companies.
이러한 상황 속에서, 배 또는 유자 추출물을 함유하여 화장료 조성물로 개발코자 하는 시도는 있어왔으나, (특허문헌 1 및 2) 배 또는 유자의 캘러스 추출물과 함께 잔탄검을 주름개선의 유효성분으로 함유시켜 주름 개선을 위한 화장료 조성물로서 개발한 사례는 전무하다. Under these circumstances, attempts have been made to develop a cosmetic composition containing a pear or citron extract (
본 발명자들은 기능성 화장품 천연 소재 개발을 위해 노력하던 중, 배 또는 유자 식물의 식물조직배양기술을 통한 식물 캘러스를 유도하였고, 이들의 추출물 또는 상등액과 잔탄검을 유효성분으로 함께 함유할 경우, 주름개선효능이 뛰어남을 확인함으로써, 본 발명을 완성하였다.The present inventors have made efforts to develop natural materials for functional cosmetics. When plant extracts or supernatant and xanthan gum were contained together as an effective ingredient, plant wrinkle improving effect The present invention has been completed.
따라서, 본 발명의 목적은 배 또는 유자 캘러스 배양물 또는 그 추출물을 함유하는 피부 외용제 조성물에 있어서, 주름 개선을 위하여 잔탄검을 유효성분으로 함유하는 주름 개선을 위한 피부 외용제 조성물을 제공하는데 있다. Accordingly, an object of the present invention is to provide a composition for external application for skin for improving wrinkles containing xanthan gum as an active ingredient for improving wrinkles in a composition for external application for skin containing a culture of bait or citron callus or an extract thereof.
본 발명의 또 다른 목적은, 상기 배 또는 유자 캘러스 배양물 또는 그 추출물 및 잔탄검을 유효성분으로 함유하는 피부 외용제 조성물의 제조방법을 제공하는데 있다. It is still another object of the present invention to provide a method for producing a composition for external application for skin, which comprises the above culture or a culture of citrus callus or an extract thereof and xanthan gum as an active ingredient.
상기와 같은 목적을 달성하기 위해, 본 발명은 In order to achieve the above object,
배 또는 유자(Magnoliales) 캘러스 배양물 또는 그 추출물을 함유하는 주름 방지 또는 개선을 위한 조성물에 있어서, 주름 방지 또는 개선을 위한 유효성분으로써 잔탄검을 포함하는 것을 특징으로 하는 피부 외용제 조성물을 제공한다. A composition for preventing or improving wrinkles containing a pear or Magnoliales callus culture or an extract thereof, which comprises xanthan gum as an active ingredient for prevention or improvement of wrinkles.
본 발명에 있어서, 상기 배 캘러스 배양물 또는 그 추출물 100 중량부에 대하여 잔탄검은 5 내지 20중량부로 합유되는 것을 특징으로 하는 피부 외용제 조성물을 제공한다. According to the present invention, there is provided an external preparation for skin comprising 5 to 20 parts by weight of xanthan gum to 100 parts by weight of the culture of the callus or extract thereof.
본 발명에 있어서, 상기 유자 캘러스 배양물 또는 그 추출물과, 잔탄검은 10:0.5 내지 2의 중량비로 합유되는 것을 특징으로 하는 피부 외용제 조성물을 제공한다. According to the present invention, there is provided a composition for external application for skin, wherein the citron callus culture or its extract and the xanthan gum are mixed at a weight ratio of 10: 0.5 to 2.
본 발명에 있어서, 상기 잔탄검은 피부외용제 조성물 전체 100중량부에 대하여 0.01 내지 1 중량부를 포함할 수 있다. In the present invention, 0.01 to 1 part by weight may be added based on 100 parts by weight of the total composition of the composition for external use of xanthan gum black skin.
본 발명에 있어서, 상기 배 캘러스 배양물 또는 그 추출물 100 중량부에 대하여, 상기 유자 캘러스 배양물 또는 그 추출물은 90 내지 110중량부, 잔탄검은 5 내지 20중량부 함유되는 것을 특징으로 하는 피부 외용제 조성물을 제공한다. The composition for external application for skin according to the present invention contains 90 to 110 parts by weight of the citrus callus culture or extract thereof and 5 to 20 parts by weight of xanthan gum to 100 parts by weight of the culture or the extract thereof. .
본 발명에 있어서, 상기 배 캘러스 배양물의 추출물은 배 캘러스 배양물을 건조하고, 분말화하여 정제수에 혼합시킨 다음, 원심분리하여 얻은 상등액이거나, In the present invention, the extract of the culture of the callus may be a supernatant obtained by drying the culture of the callus, mixing it into powder and mixing with purified water, and then centrifuging,
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 냉수 추출한 추출물이거나,The culture is dried and powdered, mixed with purified water, and then extracted with cold water,
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 열수 추출한 추출물이거나, 또는 배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 에탄올 추출한 추출물인 것을 특징으로 하는 조성물을 제공한다. The extract is an extract obtained by drying and pulverizing the culture, mixing it with purified water, and then extracting it with hot water. Alternatively, the composition is an extract obtained by drying and pulverizing the culture, mixing it with purified water, and then extracting it with ethanol.
본 발명에 있어서, 상기 유자 캘러스 배양물의 추출물은 유자 캘러스 배양물을 건조하고, 분말화하여 정제수에 혼합시킨 다음, 원심분리하여 얻은 상등액이거나, In the present invention, the extract of the citron callus culture may be a supernatant obtained by drying the citron callus culture, mixing it into powder and mixing with purified water, and then centrifuging,
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 냉수 추출한 추출물이거나,The culture is dried and powdered, mixed with purified water, and then extracted with cold water,
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 열수 추출한 추출물이거나, 또는 The culture is dried and pulverized, mixed with purified water and then extracted with hot water, or
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 에탄올 추출한 추출물인 것을 특징으로 하는 조성물을 제공한다.Wherein the extract is an extract obtained by drying and pulverizing the cultured product, mixing it with purified water, and extracting it with ethanol.
이하, 본 발명을 보다 상세히 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명은 배 또는 유자 캘러스 배양물 또는 그 추출물 및 잔탄검을 유효성분으로 함유하는 주름개선, 또는 주름 방지를 위한 피부 외용제 조성물 및 그 제조방법에 관한 것이다. The present invention relates to a composition for external application for skin for improving wrinkles or wrinkles containing a culture of a cruciferous or citron callus or extract thereof and xanthan gum as an active ingredient, and a method for producing the same.
본 발명은 일 관점에서, 배 또는 유자(Magnoliales) 캘러스 배양물 또는 그 추출물을 함유하는 주름 방지 또는 개선을 위한 조성물에 있어서, 주름 방지 또는 개선을 위한 유효성분으로써 잔탄검을 포함하는 것을 특징으로 하는 피부 외용제 조성물에 관한 것이다. 구체적으로는, 상기 배 또는 유자 캘러스 배양물 또는 그 추출물은 외용제 조성물의 총 중량에 대하여 0.1 내지 10 중량%로 함유할 수 있다. In one aspect, the present invention relates to a composition for prevention or improvement of wrinkles containing a culture of Magnolia or callus thereof or an extract thereof, which comprises xanthan gum as an active ingredient for prevention or improvement of wrinkles And an external preparation composition. Concretely, the culture of the bait or citron callus or the extract thereof may be contained in an amount of 0.1 to 10% by weight based on the total weight of the composition for external application.
본 발명에서, 배(Pyrus pyrifolia Nakai)는 배나무속(Pyrus)은 장미과의 한 속이고, 배나무속 과일을 "배"라고 부른다. 배나무의 기원지는 중국 서부, 혹은 남서부로 추정된다. In the present invention, the boat (Pyrus pyrifolia Nakai ) is a member of the pear (Pyrus) belongs to the rose family, and the fruit in the pear is called "pear". The origin of pear is estimated to be western China, or southwest.
본 발명에서, 유자(Citrus junos Siebold ex Tanaka)는 감귤과 식물로 빛깔은 밝은 노란색이고 껍질이 울퉁불퉁하다. 향기가 좋으며, 딱딱한 모과와 달리 과육이 부드러운 편이다.In the present invention, Citrus junos Siebold ex Tanaka ) is a citrus plant with bright yellow hues and rugged bark. Smell is good, unlike hard corn, the flesh is soft.
또한, 본 발명에서 배 또는 유자 캘러스 배양물 또는 그 추출물은 조성물의 총 중량에 대하여 0.1 내지 10 중량%로 함유할 수 있고, 이러한 비율은 단지 바람직한 범위일 뿐이며, 예컨대, 하기 실시예에서는 1 중량%, 또는 2중량%의 경우를 실험한 결과를 포함하고 있으나, 0.1%, 0.5%, 5%, 10%에서도 우수한 효능을 가지고 있는 것을 확인하였으며, 세포 생존율 또한 10%에서도 영향이 없음을 확인하였다. 이 이외에 20%, 30% 이상 경우도, 우수한 주름개선능을 가짐은 당업자에게 자명하다.In addition, in the present invention, the culture of the bait or citron callus or the extract thereof may be contained in an amount of 0.1 to 10% by weight based on the total weight of the composition, and this ratio is merely a preferable range. For example, , Or 2% by weight of the cells. However, it was confirmed that the cells had excellent efficacy even in 0.1%, 0.5%, 5%, and 10% cells, and the cell survival rate was not affected even at 10%. It is obvious to those skilled in the art that the wrinkle improving ability is 20% or more than 30%.
본 발명에 있어서, 상기 배 캘러스 배양물 또는 그 추출물 100 중량부에 대하여 잔탄검은 5 내지 20중량부로 합유되는 것을 특징으로 할 수 있다.In the present invention, 5 to 20 parts by weight of black xanthan gum may be mixed with 100 parts by weight of the culture of the callus or extract thereof.
상기 잔탄검은 조성물 100중량부에 대하여 0.01 내지 1 중량부로 함유할 수 있다. The composition may contain 0.01 to 1 part by weight based on 100 parts by weight of the black carbon black composition.
본 발명에 있어서, 상기 유자 캘러스 배양물 또는 그 추출물과, 잔탄검은 10:0.5 내지 2의 중량비로 합유되는 것을 특징으로 할 수 있다.In the present invention, it may be characterized in that the citron callus culture or the extract thereof is mixed with the xanthan gum at a weight ratio of 10: 0.5 to 2.
본 발명에 있어서, 상기 배 캘러스 배양물 또는 그 추출물 100 중량부에 대하여, 상기 유자 캘러스 배양물 또는 그 추출물은 90 내지 110중량부, 잔탄검은 5 내지 20중량부 함유되는 것을 특징으로 할 수 있다. In the present invention, the citrus callus culture or its extract may be contained in an amount of 90 to 110 parts by weight and 5 to 20 parts by weight, based on 100 parts by weight of the culture of the callus or the extract thereof.
일 예시로, 배캘러스와 유자캘러스 1:10 ~ 10:1 비율로 포함될 수 있다. As an example, it can be included in a ratio of 1:10 to 10: 1 between the callus and callus.
본 발명에 있어서, 상기 배 또는 유자 캘러스 배양물 (배양체) 자체를 사용하거나, 배양물을 여과하여 사용하거나, 배양물을 파쇄하여 사용하거나, 배양물을 건조시켜 분말화한 다음, 정제수에 녹여 사용할 수 있다.In the present invention, the above-mentioned embryo or callus culture (culture) itself may be used, the culture may be used by filtration, the culture may be used after being crushed, or the culture may be dried to be powdered and then dissolved in purified water .
본 발명에 있어서, "추출물"은 상기 배 또는 유자 캘러스 배양 추출물을 분말화하거나, 냉수추출법, 열수추출법, 에탄올 추출법 등 종래 알려진 다양한 추출법에 의해 수득한 추출물을 의미한다. In the present invention, "extract" means an extract obtained by various known extraction methods such as pulverization of the culture broth or citron callus extract, cold water extraction, hot water extraction and ethanol extraction.
본 발명에서 추출 방법은 특별히 제한되지 않고, 예를 들어 냉침 추출, 초음파 추출, 환류 추출, 열수 추출 등이 있다. 여기서, 열수추출의 경우, 열탕증류기에서 8～ 48시간동안, 80～ 100℃ 로 가열하여 열수 추출물을 얻는다. The extraction method in the present invention is not particularly limited, and examples thereof include cold extraction, ultrasonic extraction, reflux extraction, hot water extraction and the like. Here, in the case of hot water extraction, the hot water extract is obtained by heating at 80 to 100 ° C for 8 to 48 hours in a hot water distiller.
냉수 추출의 경우, 예컨대, 냉수 (15-25℃)에 상기 태좌조직 배양물 자체 또는 그것의 건조된 분말을 혼합하여 3일동안 추출하여 냉수추출물을 얻는다.In the case of cold water extraction, cold water (15-25 ° C), for example, is mixed with the above-mentioned placental tissue culture or its dried powder and extracted for 3 days to obtain cold water extract.
또는, 물, 유기 용매, 또는 이의 혼합 용매를 사용하여 추출하는 방법으로 제조될 수 있다. 추출한 액은 바로 사용하거나 또는 농축 및/또는 건조하여 사용할 수 있다. 유기용매를 사용하여 추출하는 경우, 메탄올, 에탄올, 이소프로판올, 부탄올, 에틸렌, 아세톤, 헥산, 에테르, 클로로포름, 에틸아세테이트, 부틸아세테이트, 디클로로메탄, N, N-디메틸포름아미드(DMF), 디메틸설폭사이드(DMSO), 1,3-부틸렌글리콜, 프로필렌글리콜 또는 이들의 혼합용매인 유기용매를 사용하며 생약의 유효 성분이 파괴되지 않거나 최소화된 조건에서 실온 또는 가온하여 추출할 수 있다. 추출하는 유기용매에 따라 약제의 유효성분의 추출정도와 손실정도가 차이가 날 수 있으므로, 알맞은 유기용매를 선택하여 사용하도록 한다. 특히, 본 발명에 있어서는 열수 추출물이거나, 에탄올 추출물, 예를 들어, 20-50% 에탄올 추출물일 수 있다.Alternatively, it can be produced by a method of extracting using water, an organic solvent, or a mixed solvent thereof. The extracted liquid can be used directly or by concentrating and / or drying. In the case of extraction using an organic solvent, an organic solvent such as methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF) (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof. The active ingredient of the herbal medicine may be extracted at room temperature or warmed under the condition that the active ingredient is not destroyed or minimized. Depending on the organic solvent to be extracted, the degree of extraction and the degree of loss of the active ingredient of the medicament may differ. Therefore, an appropriate organic solvent should be selected and used. In particular, in the present invention, it may be a hot-water extract or an ethanol extract, for example, a 20-50% ethanol extract.
본 발명에 있어서, 상기 추출물은 농축, 또는 희석하여 사용할 수 있고, 추출물의 증류액을 사용할 수도 있다.In the present invention, the above extract may be used by concentration or dilution, and a distillate of the extract may be used.
본 발명에 있어서, "유효성분으로 함유하는"의 의미는, 피부 외용제 조성물로써 주름 개선 효과를 나타낼 수 있는 정도의 유효량을 함유하는 것을 의미한다.In the present invention, the expression "comprising as an active ingredient" means that the composition for external application for skin contains an effective amount to such an extent as to exhibit a wrinkle-reducing effect.
한편, 식물조직배양은 살아있는 작은 식물 조직을 기내(in vitro)에서 무균적으로 배양하여 배양 목적에 따라서 식물 세포, 기관 및 식물체를 증식 시키는 기술이다. 식물조직배양에는 식물이 지니는 기본적인 특성을 이용한다고 볼 수 있다. 식물조직배양 기술은 식물이 지니는 독특한 특징인 식물의 체세포로부터 식물체를 재생시킬 수 있는 분화전능성(totipotency)을 이용하는 것을 기반으로 하는 기술이다. 즉, 식물의 단세포(원형질체 포함), 잎 및 뿌리 절편을 특정 영양물질 및 생장 조절제가 첨가된 배지에 배양하면 이들 세포 및 조직으로부터 식물체가 재생될 수 있다. 이러한 식물이 분화전능성(totipotency)을 지니는 능력은 식물이 지니는 특성인 고착 생활과 장기간 생존을 위하여 열악한 환경 및 초식동물의 피해로부터 생존을 하기 위한 전략이다. 따라서 식물은 잃어버린 기관을 다시 재생시키기 위한 능력을 갖게 되었다고 볼 수 있다. 모식물체로부터 분리된 식물조직세포는 적정배양환경에서 배양하면 완전한 식물체로 재생될 수 있는 잠재력 즉, 전형성능을 가지고 있는 식물은 발생학 등의 학술적인 중요성은 물론이지만 화장품 원료 등의 실용화를 위해서도 활용가치가 크다.On the other hand, plant tissue culture is a technique for cultivating asphyxiated small plant tissues in vitro to grow plant cells, organs and plants according to the purpose of culture. Plant tissue culture can be attributed to the basic characteristics of plants. Plant tissue culture technology is based on the use of differentiating totipotency to regenerate plants from plant somatic cells, a unique feature of plants. Namely, plants can be regenerated from these cells and tissues by culturing single cells (including protoplasts), leaves and root sections of plants in a medium supplemented with specific nutrients and growth regulators. The ability of these plants to have differentiating totipotency is a strategy for survival from harsh environments and herbivorous damage for the fixation and long-term survival of plants. Therefore, plants can be said to have the ability to regenerate lost organs again. The plant tissue cells isolated from the parent plant have the potential to be reproduced as a complete plant when cultured in a suitable culture environment, that is, plants having typical performance are of academic importance such as embryology, but they are also utilized for practical use of cosmetic raw materials .
본 발명은 다른 관점에서, (a) 배 또는 유자 식물로부터 캘러스 유도하는 단계; 및 (b) 상기 유도된 배 또는 유자 캘러스 배양물 또는 그 추출물 및 잔탄검을 함유하는 조성물을 제조하는 단계를 포함하는 배 또는 유자 캘러스 배양물 또는 그 추출물 및 잔탄검을 함유한 주름 개선을 위한 피부 외용제 조성물의 제조방법에 관한 것이다. In another aspect, the present invention provides a method for producing a callus, comprising: (a) inducing callus from a pear or citron plant; And (b) a step of preparing a composition containing the derived bait or citron callus culture or an extract thereof and xanthan gum, or a composition for external application for wrinkles containing waxy callus cultures or extracts thereof and xanthan gum And a method for producing the same.
상기 (a)단계에서, 구체적으로는 배 또는 유자 캘러스를 배양하기 위해 적절한 배지를 선택할 수 있다. 기술 분야에서 식물조직배양에 일반적으로 사용되고 있는 배지가 있다면, 제한 없이 사용가능하다. 식물에서는 일반적으로 MS배지, B5배지 등을 주로 사용하고, 일 예로, MS배지의 조성(1L기준시)은 NH4NO3 1650 mg, KNO3 1900 mg, CaCl2.2H2O 440 mg, MgSO4.7H2O 370 mg, KH2PO4 170 mg, KI 0.83 mg, H3BO3 6.2 mg, MnSO4.4H2O 22.3 mg, ZnSO4.7H2O 8.6 mg, Na2MoO4.2H2O 0.25 mg, CuSO4,5H2O 0.025 mg, CoCl2.6H2O 0.025 mg, FeSO4.7H2O 27.8 mg, Na2EDTA.2H2O 37.3 mg, Myoinositol 100 mg, Nicotinic acid 0.5 mg, Pyridoxine-HCl 0.5 mg, Thiamine-HCl 0.5 mg, Glycine 2 mg, Sucrose 30000 mg 이다. In the step (a), it is possible to select a suitable medium for culturing the embryo or citrus callus. If there is a medium commonly used in plant tissue culture in the technical field, it can be used without limitation. For example, the composition of the MS medium (1 L standard) is 1650 mg NH 4 NO 3 , 1900 mg KNO 3 , 440 mg CaCl 2 .2H 2 O,
본 발명에 있어서, 상기 (b)단계에서 있어서, (a)단계의 배양을 통해 얻어진 배 또는 유자 캘러스 배양물 그대로를 포함하는 조성물을 적절한 형태의 피부 외용제 조성물로 제조하거나, 또는 상기 배양물을 공지된 추출 방법을 통해 추출물 형태로 수득하여 적절한 형태의 피부 외용제 조성물로 제조할 수 있다.In the present invention, it is preferable that in the step (b), a composition comprising the culture as obtained from the culture of step (a) as it is or the culture of citron callus as it is is prepared from a suitable external preparation for skin composition, And can be prepared in the form of an external preparation for skin in a suitable form.
예컨대, 상기 (b)단계는, (a)단계에서 얻어진 배 또는 유자 캘러스 배양물을 건조하고, 분말화하여 정제수에 혼합시켜 조성물을 제조하거나, For example, in the step (b), the culture of the bait or citron callus obtained in step (a) is dried, powdered and mixed with purified water to prepare a composition,
(a)단계에서 얻어진 배 또는 유자 캘러스 배양물을 건조하고, 분말화하여 정제수에 혼합시킨 다음, 냉수 추출, 열수 추출 또는 에탄올 추출하여 조성물을 제조하는 단계를 포함할 수 있다. the step of drying the mixture of the bait or citron callus obtained in step (a), pulverizing it, mixing with purified water, and then preparing the composition by cold extraction, hot water extraction or ethanol extraction.
특히, 상기 제조 방법에 있어서, 예컨대, (b)단계에서, 피부외용제 조성물에 주름개선을 위한 유효성분으로써 잔탄검을 추가하여 조성물에 함유시키는 단계를 더 포함한다. Particularly, in the above production method, for example, the step (b) further comprises adding xanthan gum as an effective ingredient for improving wrinkles to the composition for external application for skin, and then adding the composition to the composition.
또는, 상기 제조방법에 있어서, (a)단계에서, 잔탄검을 캘러스를 위한 배지 조성물에 추가로 함유시켜 배 또는 유자 캘러스를 유도 및/또는 배양하는 단계를 포함할 수 있다.Alternatively, the production method may further comprise, in step (a), further comprising a xanthan gum in a medium composition for callus to induce and / or cultivate a bait or citron callus.
본 발명에 있어서, 상기 피부 외용제 조성물은 화장료 조성물 또는 약제학적 조성물일 수 있다.In the present invention, the composition for external application for skin may be a cosmetic composition or a pharmaceutical composition.
상기 화장료 조성물에 있어서는, 화장품 제제에 있어서 수용가능한 담체를 포함할 수 있다. 여기서, "화장품 제제에 있어서 수용가능한 담체"란 화장품 제제에 포함될 수 있는 이미 공지되어 사용되고 있는 화합물 또는 조성물이거나 앞으로 개발될 화합물 또는 조성물로서 피부와의 접촉시 인체가 적응 가능한 이상의 독성, 불안정성 또는 자극성이 없는 것을 말한다.The cosmetic composition of the present invention may contain an acceptable carrier in cosmetic preparations. Herein, "an acceptable carrier for a cosmetic preparation" is a known or used compound or composition that can be contained in a cosmetic preparation, or a compound or composition to be developed in the future, which is toxic, instable or irritating It says nothing.
상기 담체는 본 발명의 피부 외용제 조성물에 그것의 전체 중량에 대하여 약 1 중량 % 내지 약 99.99 중량 %, 바람직하게는 조성물의 중량의 약 90 중량% 내지 약 99.99 중량 %로 포함될 수 있다. 그러나 상기 비율은 본 발명의 피부 외용제 조성물이 제조되는 후술한 바의 제형에 따라 또 그것의 구체적인 적용 부위(얼굴, 목 등)나 그것의 바람직한 적용량 등에 따라 달라지는 것이기 때문에, 상기 비율은 어떠한 측면으로든 본 발명의 범위를 제한하는 것으로 이해되어서는 안 된다.The carrier may be included in the skin topical composition of the present invention in an amount of from about 1% by weight to about 99.99% by weight, preferably from about 90% by weight to about 99.99% by weight of the composition, based on the total weight thereof. However, since the ratio varies depending on the formulations described below in which the composition for external application for skin of the present invention is prepared, and its specific application site (face, neck, etc.) or its preferable application amount and the like, And should not be construed as limiting the scope of the invention.
상기 담체로서는 알코올, 오일, 계면활성제, 지방산, 실리콘 오일, 습윤제, 보습제, 점성 변형제, 유제, 안정제, 자외선산란제, 자외선흡수제, 발색제, 향료 등이 예시될 수 있다. 상기 알코올, 오일, 계면활성제, 지방산, 실리콘 오일, 습윤제, 보습제, 점성 변형제, 유제, 안정제, 자외선산란제, 자외선흡수제, 발색제, 향료로 사용될 수 있는 화합물/조성물 등은 이미 당업계에 공지되어 있기 때문에 당업자라면 적절한 해당 물질/조성물을 선택하여 사용할 수 있다.Examples of the carrier include an alcohol, an oil, a surfactant, a fatty acid, a silicone oil, a wetting agent, a moisturizer, a viscous modifier, an emulsifier, a stabilizer, an ultraviolet scattering agent, an ultraviolet absorber, a coloring agent and a perfume. The compounds / compositions which can be used as the above-mentioned alcohol, oil, surfactant, fatty acid, silicone oil, humectant, humectant, viscous modifier, emulsifier, stabilizer, ultraviolet scattering agent, ultraviolet absorber, The person skilled in the art can select and use the appropriate substance / composition.
본 발명의 일 구현예로써, 본 발명에 따른 피부 외용제 조성물은 상기 배 또는 유자 캘러스 배양 추출물 이외에 글리세린, 부틸렌글리콜, 프로필렌글키롤, 폴리옥시에틸렌 경화피마자유, 에탄올, 트리에탄올아민 등을 포함할 수 있으며, 방부제, 항료, 착색료, 정제수 등을 필요에 따라 미량 포함할 수 있다. As an embodiment of the present invention, the composition for external application for skin according to the present invention may contain glycerin, butylene glycol, propylene glycol, polyoxyethylene hardened castor oil, ethanol, triethanolamine and the like in addition to the above- Preservatives, antioxidants, coloring agents, purified water, etc. can be included as needed.
본 발명에 따른 피부 외용제 조성물은, 다양한 형태로 제조될 수 있는데, 예컨대, 화장수, 에센스, 젤, 에멀젼, 로션, 크림(수중유적형, 유중수적형, 다중상), 용액, 현탁액(무수 및 수계), 무수 생성물(오일 및 글리콜계), 젤, 마스크, 팩, 분말, 또는 젤라틴 등의 피막이 있는 캅셀 (소프트 캅셀, 하드 캅셀) 제형 등의 형태로 제조될 수 있다. The composition for external application for skin according to the present invention can be prepared in various forms such as lotion, essence, gel, emulsion, lotion, cream (underwater type, water type, multiphase), solution, suspension ), Capsules (soft capsules, hard capsules) having a coating such as anhydrous products (oil and glycol), gel, mask, pack, powder or gelatin.
본 발명에 있어서의 피부는 얼굴 뿐만 아니라, 두피, 전신도 포함되는 개념으로, 이러한 두피에 적용될 수 있는 피부 외용제 조성물로써, 샴푸, 린스, 트리트먼트, 발모제 등이 있고, 전신에 적용될 수 있는 바디클렌져 등의 용도로써 다양한 형태로 제조될 수 있다. The skin of the present invention includes not only the face but also the scalp and whole body. The composition for external application for skin which can be applied to such scalp includes shampoo, rinse, treatment, hair removal agent, etc., and a body cleanser And the like can be manufactured in various forms.
본 발명에 따른 배 또는 유자 캘러스 배양 추출물 및 잔탄검을 함유 피부 외용제 조성물의 제조방법은 전술한 제조방법에 한정되는 것은 아니며, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자라면 상기 제조방법을 일부 변형시킨 방법으로도 본 발명에 따른 배 또는 유자 캘러스 배양 추출물을 함유 피부 외용제 조성물을 제조할 수 있다.The method for preparing a composition for external application for skin containing crucifer or callus culture according to the present invention and the composition containing xanthan gum is not limited to the preparation method described above. The composition for external application for skin containing the culture extract of the crucifer or callus of the present invention may be prepared by a modified method.
특히, 상기 피부 외용제 조성물은 본 발명에 특별히 개시된 제조방법 이외에도, 통상적으로 알려진 제조방법을 이용하여, 일반적인 유화 제형 및 가용화 제형의 형태로 제조될 수 있다. In particular, the composition for external application for skin may be prepared in the form of a general emulsified formulation and a solubilized formulation, by a known manufacturing method, in addition to the manufacturing method specifically disclosed in the present invention.
화장료 조성물로 제조될 경우, 유화 제형의 화장품으로는 영양화장수, 크림, 에센스 등이 있으며, 가용화 제형의 화장품으로는 유연화장수가 있다. 또한, 피부과학적으로 허용가능한 매질 또는 기제를 함유함으로써 피부과학 분야에서 통상적으로 사용되는 국소적용 또는 전신적용할 수 있는 보조제 형태로 제조될 수 있다. When the cosmetic composition is manufactured from a cosmetic composition, the cosmetic composition of the emulsified formulation includes nutritional lotion, cream, essence and the like. It can also be prepared in the form of adjuvants which can be applied topically or systemically, which are conventionally used in the field of dermatology by containing a dermatologically acceptable medium or base.
또한, 적합한 화장품의 제형으로는, 예를 들면 용액, 겔, 고체 또는 반죽 무수 생성물, 수상에 유상을 분산시켜 얻은 에멀젼, 현탁액, 마이크로에멀젼, 마이크로캡슐, 미세과립구 또는 이온형(리포좀), 비이온형의 소낭 분산제의 형태, 크림, 스킨, 로션, 파우더, 연고, 스프레이 또는 콘실스틱의 형태로 제공될 수 있다. 또한, 포말(foam)의 형태 또는 압축된 추진제를 더 함유한 에어로졸 조성물의 형태로도 제조될 수 있다. In addition, suitable cosmetic formulations include, for example, solutions, gels, solid or kneaded anhydrous products, emulsions obtained by dispersing an oil phase in water, suspensions, microemulsions, microcapsules, microgranules or ionic forms (liposomes) May be provided in the form of a follicular dispersing agent of the type, cream, skin, lotion, powder, ointment, spray or conical stick. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.
또한, 본 발명의 피부 외용제 조성물은 추가로 지방 물질, 유기 용매, 용해제, 농축제 및 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온봉쇄제 및 킬레이트화제, 보존제, 비타민, 차단제, 습윤화제, 필수 오일,염료, 안료, 친수성 또는 친유성 활성제, 지질 소낭 또는 화장품에 통상적으로 사용되는 임의의 다른 성분과 같은 화장품학 또는 피부과학 분야에서 통상적으로 사용되는 보조제를 함유할 수 있다. 그리고, 상기의 성분들은 피부과학 분야에서 일반적으로 사용되는 양으로 도입될 수 있다.In addition, the composition for external application for skin of the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickeners and gelling agents, softening agents, antioxidants, suspending agents, stabilizers, foaming agents, As used herein means any emulsion or emulsion which is commonly used in emulsifying or emulsifying agents, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics Adjuvants commonly used in the cosmetics or dermatological fields, such as other ingredients. And, the above ingredients can be introduced in amounts commonly used in the field of dermatology.
이러한, 본 발명에 따른 피부 외용제 조성물은 콜라겐 합성 증진능에 따른 피부 노화 방지 등의 기능성 화장품의 형태를 포함한다.The composition for external application for skin according to the present invention includes forms of functional cosmetics such as skin aging prevention due to the ability to promote collagen synthesis.
본 발명에 따른 피부 외용제 조성물은, 특히 잔탄검을 주름 개선을 위한 유효성분으로써 배 또는 유자 캘러스 배양물 또는 그 추출물과 함께 함유함으로써, 콜라겐 합성에 중요한 프롤린 히드록실라제 및 라이실 히드록실라제 효소들의 발현을 증가시켜 우수한 주름 개선 효과를 가지고 있어, 기능성 화장품 소재로써 활용가치가 높다. The composition for external application for skin according to the present invention is characterized in that xanthan gum is contained as an active ingredient for improving wrinkles and is contained together with a bait or citron callus culture or an extract thereof so that proline hydroxylase and lysylhydroxylase enzyme And it has a wrinkle-improving effect, and thus it is highly useful as a functional cosmetic material.
도 1은 다양한 농도의 배 캘러스 추출물 및 유자 캘러스 추출물의 단독 및 혼합 처리가 proline hydroxlase와 lysyl hydroxylase 효소 발현에 미치는 효과를 나타낸 결과이다. (No. 1: 무처리 대조군, No. 2: 배 캘러스 추출물 0.1%, No. 3: 배 캘러스 추출물 0.5%, No. 4: 배 캘러스 추출물 1.0%, No. 5: 유자 캘러스 추출물 0.1%, No. 6: 유자 캘러스 추출물 0.5%, No. 7: 배 캘러스 추출물 1.0%, No. 8: 배 캘러스 추출물 0.5% + 유자 캘러스 추출물 0.5%)
도 2는 배 캘러스 추출물, 유자 캘러스 추출물 및 잔탄검의 혼합 처리가 proline hydroxylase와 lysyl hydroxylase의 발현에 미치는 효과를 나타낸 결과이다. (No. 1: control, No. 2: 잔탄검 0.01%, No. 3: 잔탄검 0.05%, No. 4: 잔탄검 0.1%, No. 5: 잔탄검 0.25%, No. 6: 잔탄검 0.5%, No. 7: 배 캘러스 추출물 1.0% + 잔탄검 0.1%, No. 8: 유자 캘러스 추출물 1.0% + 잔탄검 0.1%, No. 9: 배 캘러스 추출물 0.5% + 유자 캘러스 추출물 0.5% + 잔탄검 0.1%)
도 3은 배 캘러스 추출물 0.5%, 유자 캘러스 추출물 0.5%, 잔탄검 0.1%의 혼합처리가 섬유아세포의 collagen 생산에 미치는 영향을 나타낸 결과이다. (1. 잔탄검 0.1% 처리군, 2. 유자 캘러스 추출물 1.0% + 잔탄검 0.1% 혼합 처리군, 3. 배 캘러스 추출물 1.0% + 잔탄검 0.1% 혼합 처리군, 4. 유자 캘러스 추출물 0.5% + 배 캘러스 추출물 0.5% + 잔탄검 0.1% 혼합 처리군, 5. Positive (collagen) 대조군, 6 Negative 대조군)Figure 1 shows the effect of single and mixed treatment of various concentrations of the extracts of the callus and callus extracts on proline hydroxlase and lysyl hydroxylase enzyme expression. (No. 1: untreated control group, No. 2: bacillus extract 0.1%, No. 3: bacillus extract 0.5%, No. 4: bacillus extract 1.0%, No. 5: citron extract 0.1% 6: extract of citron extract 0.5%, No. 7: extract of callus extract 1.0%, No. 8: extract of callus 0.5% + extract of citron extract 0.5%)
FIG. 2 shows the effect of the mixed treatment of Baccarus extract, Yuza callus extract and xanthan gum on the expression of proline hydroxylase and lysyl hydroxylase. (No. 1: control, No. 2: xanthan gum 0.01%, No. 3: xanthan gum 0.05%, No. 4: xanthan gum 0.1%, No. 5: xanthan gum 0.25%, No. 6: xanthan gum 0.5 %, No. 7: Boar callus extract 1.0% + xanthan gum 0.1%, No. 8: citron extract 1.0% + xanthan gum 0.1%, No. 9: callus extract 0.5% + citron extract 0.5% + xanthan gum 0.1%)
FIG. 3 shows the effect of the mixed treatment of 0.5% of the extract of Baccarus extract, 0.5% of citrus fruit extract and 0.1% of xanthan gum on the collagen production of fibroblasts. (1) xanthan gum 0.1% treated group, 2. yucca callus extract 1.0% + xanthan gum 0.1% mixed treatment group, 3. xylane extract 1.0% + xanthan gum 0.1% mixed group, 4. yuzu callus extract 0.5% 0.5% mixed extract of xylitol and 0.1% xanthan gum, 5. Positive (collagen) control, 6 Negative control)
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지 않는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.
특히, 이하의 실시예에서는 배 또는 유자 캘러스 배양 추출물, 잔탄검에 관한 효능을 확인하였으나, 추출물이 아닌 배양물 자체에 대해서도 이러한 효과가 있음은 당업자에게 자명할 것이다.Particularly, in the following examples, the efficacy of the extracts of xanthographed callus and callus cultivar and xanthan gum was confirmed, but it will be obvious to those skilled in the art that such effects are also obtained not only in the extract but also in the culture itself.
1. 본 발명에 따른 배 및 유자의 ≪ RTI ID = 0.0 > 1. < / RTI > 식물캘러스Plant callus 유도 및 배양 Induction and culture
(1) 식물재료 준비(1) Plant material preparation
유자 캘러스 유도를 위한 식물체는 이삭농원에서 구입한 유자 묘목의 잎과 열매의 과즙, 씨를 사용하였다. 배 캘러스 유도를 위한 식물체는 좋은영농조합에서 받은 씨앗을 발아시켜 사용하였다. 70% EtOH (Merck, cat.603-002-00-5) 용액을 이용해 1분 30초간 살균처리 한 후, 2% sodium hypochloride (Duksan, cat. 7681-52-9) 용액에서 30분간 교반 하였다. 그 다음 클린벤치 내에서 멸균 된 증류수로 4회 반복 세척하였다. 멸균 처리한 후 식물 조직 샘플들을 이용하여 아래와 같이 유자 및 캘러스를 유도하였다.The plants used for the induction of citrus callus were the leaves and fruit juice, seeds of citron seedlings purchased from Isaac plantation. Plants for inducing the callus were germinated seeds from a good farming association. After sterilization for 1 minute and 30 seconds with 70% EtOH (Merck, cat.603-002-00-5) solution, the mixture was stirred for 30 minutes in 2% sodium hypochloride (Duksan, cat. 7681-52-9) solution. It was then washed 4 times with sterile distilled water in a clean bench. After sterilization, citron and callus were induced using plant tissue samples as described below.
(2) 유자 및 배 캘러스 유도(2) induction of citrus and pear callus
유자 및 배의 잎, 과즙, 씨를 각각 일정한 크기로 잘라 상처 낸 후, 캘러스 유도 배지에 치상하였다. 유자 및 배 캘러스의 유도를 위하여 기본 MS 배지 (Murashige and Skoog, Duchefa Cat. M0222.0050)에 각각 2,4-dichlorophenoxy-acetic Acid (2,4-D, Duchefa Cat. D0911.0100)와 6-Benzylaminopurine (BA, Duchefa Cat. B0904.0001) 1 mg/L가 들어있는 배지들을 사용하였으며, 암주기와 16시간 광주기로 유도하였다 (도 1). Citrus and pear leaves, juice, and seeds were cut to a certain size, respectively, and wounded. 2,4-dichlorophenoxy-acetic acid (2,4-D, Duchefa Cat. D0911.0100) and 6-dichlorophenoxy-acetic acid were added to the basal MS medium (Murashige and Skoog, Duchefa Cat. M0222.0050) Medium containing 1 mg / L of Benzylaminopurine (BA, Duchefa Cat. B0904.0001) was used and induced into the cancer cycle and 16 hour photoperiod (Fig. 1).
(3) 유자 및 배 캘러스 성장 조건 탐색(3) Exploring growth conditions of yuzu and pear callus
유도된 유자 및 배 캘러스를 고체상 배지에서 성장시키기 위해 기본 MS 배지에 식물 캘러스 성장에 주로 사용되는 Picloram (Duchefa, Cat. P0914.0005) 2 mg/L 농도로 넣은 배지를 사용하였다. To induce citron and pear callus growth on solid medium, medium supplemented with 2 mg / L of Picloram (Duchefa, Cat. P0914.0005), which is mainly used for plant callus growth, was used in primary MS medium.
(4) 유자 및 배 캘러스 액체 배양(4) citron and pear callus liquid culture
고체 배지에서 성장된 유자 및 배 캘러스의 배양 scale-up을 위하여 고체 배지 상 배양의 단계를 액체 배지 배양 단계로 전환하였다. 이를 위해 고체 배지 상에서 확인된 최적화된 배양조건을 활용하여 Picloram 2 mg/L를 추가한 MS 기본배지 100ml을 500ml 삼각 플라스크에 넣어 잘 배양된 고체 배양 배지 (plate) 3개 양의 유자 캘러스를 접종하고 25℃, 100rpm에서 배양하였다 (도 2). For the scale-up of cultures of citron and bacillus grown in solid medium, the steps of solid-medium culture were converted to a liquid medium culture step. For this, 100 ml of MS basic medium supplemented with 2 mg / L of Picloram was placed in a 500 ml Erlenmeyer flask using optimized culture conditions on solid medium and inoculated with three well-cultured solid culture plates in the amount of citron callus And cultured at 25 DEG C and 100 rpm (Fig. 2).
2. 유자와 배 2. Yuzu and Pear 캘러스Callus 추출 시료 준비 Preparation of extraction samples
상기 1에서 얻어진 유자 캘러스 및 배 캘러스 배양물을 가지고, With the citron and callus cultures obtained in 1 above,
첫 번째로, 캘러스를 파쇄를 통해 냉장 상태에서 추출하는 추출물("캘러스 상등액 추출물"), 두 번째로, 캘러스를 파쇄한 후 물의 중탕 가열을 통해 열수 추출하는 추출물("캘러스 열수 추출물")로 나누어 각각 얻었다.First, the callus is divided into the extract ("callus supernatant extract") which is extracted from the cold state by crushing, the second is the extract ("callus hot water extract") which is obtained by crushing the callus and then hot- Respectively.
좀 더 자세하게는, 막자사발에 유자 캘러스를 넣어 액체질소를 부어주며 곱게 갈았다. 유자 혹은 배 캘러스 0.2 g당 PBS 1 ml을 넣어 주고 잘 섞이게 4℃ 에서 1시간이상 교반시킨 후, 위에서 언급한 2가지로 구별하여 샘플들을 준비하였다. In more detail, we poured liquid nitrogen into the mullet bowl and put it on finely ground. 1 ml of PBS per 0.2 g of citron or pear callus was added, and the mixture was stirred well at 4 ° C for 1 hour or more.
첫째, 유자 혹은 배 캘러스 상등액 추출물의 준비는 교반된 샘플을 바로 8000 rpm에서 10분간 원심분리한 후 상등액 취해 0.8 um syringe filter를 사용하여 여과하여 시료를 준비하였다 ("캘러스 상등액 추출물"). First, the preparation of the citron or bacillus supernatant extract was carried out by centrifuging the agitated sample immediately at 8000 rpm for 10 minutes, taking the supernatant and filtering it using a 0.8 μm syringe filter ("callus supernatant extract").
둘째, 유자 혹은 배 캘러스 열수 추출물의 준비는 교반된 샘플을 물중탕(90~95 ℃)으로 2시간 가열한 후 8000rpm에서 10분간 원심 분리한 후 상등액 취해 0.8 um syringe filter를 사용하여 여과하여 시료를 준비하였다 ("캘러스 열수 추출물"). Second, the preparation of hot water extract of citrus or pear callus was performed by heating the stirred sample in water bath (90 ~ 95 ℃ ) for 2 hours, centrifuging at 8000 rpm for 10 minutes, taking the supernatant and filtering with 0.8 um syringe filter. ("Callus hydrothermal extract").
3. 배 캘러스 추출물, 유자 캘러스 추출물 및 잔탄검 혼합물 준비3. Preparation of Baccaralus extract, citron extract and xanthan gum mixture
상기 2에서 얻어진 배 캘러스 열수 추출물, 유자 캘러스 열수 추출물 및 잔탄검(Sigma사)을 혼합한 시료롤 준비하였다. 아래에 기재되는 시료의 함량 %는 전체 조성물 중량(부피)에 대한 함량 % 로 기재하였다.A sample roll prepared by mixing the hot water extract of Baccarry, hot water extract of Yuzu callus and xanthan gum (Sigma) obtained in 2 above was prepared. The content% of the samples described below is expressed as a percentage content with respect to the total composition weight (volume).
실험예 1: 본 발명에 따른 피부 외용제 조성물의 주름 개선 효능 시험 Experimental Example 1: Effect of wrinkle-improving effect of the composition for external application for skin according to the present invention
인체 피부를 구성하는 섬유아세포 (fibroblast)가 생산하는 세포간 물질 중 피부의 탄력을 유지하는 핵심 성분인 콜라겐 (collagen)은 세포 내 합성에 proline 아미노산의 유도체인 hydorxyproline과 lysin 아미노산의 유도체인 hydroxyllysin이 필수 요소로 요구된다. 이 두 아미노산 유도체들은 proline hydroxlase와 lysyl hydroxylase 효소들에 의해 효소 전환되는데, 섬유아세포의 세포 배양시 배 캘러스 추출물, 유자 캘러스 추출물, 그리고 xanthan gum 혼합 처리가 세포내 두 효소의 발현에 어떤 영향을 미치는 지 RT-PCR로 조사하였다.Collagen, a key component of the skin's elasticity, is produced by fibroblasts that constitute the skin of the body. It is composed of hydorxyproline, a derivative of proline amino acid, and hydroxyllysin, a derivative of lysin amino acid. Element. These two amino acid derivatives are converted to enzymes by proline hydroxlase and lysyl hydroxylase enzymes. In the cell culture of fibroblasts, the effect of the combination of bacillus extract, citron extract, and xanthan gum on the expression of two enzymes in the cell RT-PCR.
1.1 CCD-986sk 피부 섬유아세포 배양1.1 CCD-986sk skin fibroblast culture
CCD-986sk 피부 섬유아세포 (Human dermal fibroblast cell)는 Polystyrene 세포 배양접시에 부착시키고 10% FBS (Gibco, USA)를 첨가한 DMEM (Gibco, USA)을 사용하여 배양하였다. 배양시 습도는 95%, 온도는 37℃를 유지 하면서 5% CO2를 계속 공급하였다.CCD-986sk human dermal fibroblast cells were cultured in DMEM (Gibco, USA) supplemented with 10% FBS (Gibco, USA) in a Polystyrene cell culture dish. During the incubation, 5% CO2 was continuously supplied while maintaining the humidity at 95% and the temperature at 37 ° C.
1.2 Reverse transcriptase PCR을 이용한 효소의 mRNA 발현 측정1.2 Measurement of enzyme mRNA expression using reverse transcriptase PCR
각 농도의 배 캘러스 추출물, 유자 캘러스 추출물, 잔탄검을 단독 혹은 혼합으로 2일 또는 3일간 처리한 세포에서 Trizol reagent를 이용하여 RNA를 분리하고 파장 260 nm에서 정량하였다. Total RNA 10 μL (0.5μg/μL)를 65℃에서 10분간 가열하고 4℃에서 10분 동안 방치한 후 1 μL oligo (dT)15 primer (0.5 μg/μL), 1μL M-MLV RT (10 unit/μL; promega, USA), 1 μL RNase inhibitor (20~40 unit/μL; promega, USA), 10μL 5X RT buffer (250 mM Tris-HCl, pH8.3, 375 mM KCl, 15 mM MgCl2), 5 μL 2.5 mmol/L dNTP mixture, 0.1% diethylpyrocarbonate (DEPC) 증류수를 첨가하고 37℃에서 1시간 동안 가열하여 cDNA를 합성하였다. Prolyl hydroxylase와 Lysyl hydroxylase의 mRNA 발현량을 측정하기 위하여 합성된 cDNA 로 RT-PCR을 실시하였다. 사용된 GAPDH (Glyceraldehyde-3-phosphate dehydrogenase), Prolyl hydroxylase와 Lysyl hydroxylase의 primer sequence는 Table 1과 같다. Prolyl hydroxylase의 primer를RNA was isolated from the cells treated with each concentration of Baccarus extract, citron extract, and xanthan gum alone or mixed for 2 or 3 days using Trizol reagent and quantified at a wavelength of 260 nm. Total RNA (10 μL, 0.5 μg / μL) was heated at 65 ° C. for 10 minutes and allowed to stand at 4 ° C. for 10 minutes. Thereafter, 1 μL oligo (dT) 15 primer (0.5 μg / μL) and 1 μL M- 5 mM RT buffer (250 mM Tris-HCl, pH 8.3, 375 mM KCl, 15 mM MgCl2), 5 μl of RNase inhibitor (20-40 units / μL; promega, USA) μL 2.5 mmol / L dNTP mixture, 0.1% diethylpyrocarbonate (DEPC) distilled water was added and the mixture was heated at 37 ° C for 1 hour to synthesize cDNA. RT-PCR was performed on the synthesized cDNA to measure the mRNA expression levels of Prolyl hydroxylase and Lysyl hydroxylase. Table 1 shows the primer sequences of GAPDH (Glyceraldehyde-3-phosphate dehydrogenase), Prolyl hydroxylase and Lysyl hydroxylase. Primer of Prolyl hydroxylase
각각 1 μL (0.5 μg/μL)씩 가하고 합성된 cDNA에 0.1% DEPC-Water와 함께 PCR-premix tube ((주)바이오니아) 에 첨가하여 Denaturation은 95℃에서 30초, annealing은 72℃에서 30초, extension은 72℃에서 2분동안 30cycle로 진행하였다. Lysyl hydroxylase는 denaturation 94℃ 30초, annealing 52℃에서 45초, extention 72℃ 30초간 총 40cycle로 진행하였다. PCR 생성물은 2% agarose gel에서 non-specific PCR product나 primer dimer 등이 없는지를 확인하였고, 생성물의 양을 imaging densitometer [Labworks ver.4.6. (image acquisition and analysis software), UVP, CA]를 사용하여 측정하였다.The denaturation was carried out at 95 ° C for 30 seconds and the annealing was carried out at 72 ° C for 30 seconds. The PCR was carried out by adding 1 μL (0.5 μg / μL) , and the extension was carried out at 72 ° C for 2 minutes for 30 cycles. Lysyl hydroxylase was denaturation at 94 ℃ for 30 sec, annealing at 52 ℃ for 45 sec and extention 72 ℃ for 30 sec. The PCR product was confirmed to be non-specific PCR product or primer dimer in 2% agarose gel, and the amount of product was measured using an imaging densitometer [Labworks ver.4.6. (image acquisition and analysis software, UVP, CA).
실험 결과, 프롤린 하이드록실라제(proline hydroxlase) 발현의 경우 배 캘러스 추출물과 유자 캘러스 추출물을 세포 배양액을 기준으로 각각 단독으로 0.1%, 0.5%, 그리고 1.0% 또, 0.5%씩 혼합하여 처리한 경우 무처리 군에 비해 증가하는 경향을 보였으나, 그 증가가 유의적이지는 않았다 (도 1). 역시 라이실 하이드록실라제(lysyl hydroxylase) 발현의 경우도 배 캘러스 추출물과 유자 캘러스 추출물을 세포 배양액을 기준으로 각각 단독으로 0.1%, 0.5%, 그리고 1.0% 또, 0.5%씩 혼합하여 처리한 경우 무처리 군에 비해 큰 변화를 유발하지 못하였다 (도 1).In the case of proline hydroxlase expression, the extracts of the calli extract and citron extract were mixed with 0.1%, 0.5%, 1.0%, and 0.5% , But the increase was not significant (Fig. 1). Also, in the case of lysyl hydroxylase expression, the extracts of calli and callus extracts were treated with a mixture of 0.1%, 0.5%, 1.0%, and 0.5% (Fig. 1). ≪ tb > < TABLE >
그러나, 배 캘러스 추출물과 유자 캘러스 추출물과 함께 0.1% 농도의 xanthan gum을 혼합 처리하는 경우 proline hydroxlase와 lysyl hydroxylase 발현들이 무처리구 대조군 혹은 배 캘러스 추출물과 유자 캘러스 추출물의 처리군에 비해 유의적으로 증가됨을 확인 할 수 있었다 (도 2).However, proline hydroxlase and lysyl hydroxylase expression were significantly increased in the mixed treatment of xanthan gum with 0.1% concentration of callus extract and citrus callus extract compared to the untreated control group or bacillus extract and citrate extract group (Fig. 2).
이러한 실험 결과를 통해 배 캘러스 추출물과 유자 캘러스 추출물은 시험된 농도 범위에서 단독 처리의 경우, 콜라겐을 합성하는데 중요한 두 효소의 세포내 유전자 발현에 큰 영향을 미치지 않았으나 0.1%의 잔탄검과 혼합 처리하는 경우, 유의적인 발현양의 증가가 확인되었다. 실험 결과 잔탄검만을 단독으로 처리하는 경우는 시험에 사용된 0.01%에서 0.5%까지 무처리군에 비해 유의적인 차이가 없었지만, proline hydroxylase 발현양은 배 캘러스 추출물 1.0%와 잔탄검 0.1%를 혼합 처리한 경우 무처리군에 비해 약 149%와 유자 캘러스 추출물 1.0%와 잔탄검 0.1%를 혼합 처리한 경우는 약 168% 증가한 결과를 보였으며, 이러한 잔탄검의 효과는 배 캘러스 추출물과 유자 캘러스 추출물을 0.5%씩 혼합 처리한 경우 약 237%로 크게 증가하였다.These results suggest that the extracts of Bacillus subtilis and Yuzu callus extract did not significantly affect intracellular gene expression of the two enzymes important for the synthesis of collagen in the concentration range tested but mixed with 0.1% , An increase in a significant expression level was confirmed. The results showed that the treatment of xanthan gum alone was not significantly different from that of the untreated group from 0.01% to 0.5%, but the amount of proline hydroxylase expressed was 1.0% of xylose extract and 0.1% of xanthan gum The extracts of Bacillus subtilis and Yuza callus extracts were found to be 0.5% and 0.8%, respectively, compared with the untreated group. %, Respectively, which was about 237%.
Lysyl hyroxylase의 발현양에 미치는 영향도 proline hydroylase의 발현양에 대한 효과와 유사하였다. 실험 결과 잔탄검만을 단독으로 처리하는 경우는 시험에 사용된 0.01%에서 0.5%까지 무처리군에 비해 유의적인 차이가 없었지만, lysyl hydroxylase 발현양은 배 캘러스 추출물 1.0%와 잔탄검 0.1%를 혼합 처리한 경우 무처리군에 비해 약 129%와 유자 캘러스 추출물 1.0%와 잔탄검 0.1%를 혼합 처리한 경우는 약 142% 증가한 결과를 보였으며, 이러한 잔탄검의 효과는 배 캘러스 추출물과 유자 캘러스 추출물을 0.5%씩 혼합 처리한 경우 약 248%로 크게 증가하였다.The effect of Lysyl hyroxylase on the expression level was similar to that of proline hydrolyase. As a result, the concentration of 0.01% to 0.5% of xanthan gum solely treated with xanthan gum alone was not significantly different from that of the untreated group, but the expression of lysyl hydroxylase was 1.0% and xanthan gum 0.1% The effect of xanthan gum was significantly higher than that of untreated group. The effects of xanthan gum extract and yuzu callus extract were found to be 0.5 %, Respectively, which was about 248%.
1.3 콜라겐 생성 촉진 활성 확인 시험1.3 Confirmation test for promoting collagen production
상기의 방법으로 개발된 유자 및 배 식물캘러스 상등액 및 추출액의 콜라겐 생성 촉진 활성은 다음과 같이 평가하였다. 인간섬유아세포 (human fibroblast cell, CCD-986sk)를 96 well plate에 1 × 104 cell/well씩 분주하고 10% FBS가 포함된 DMEM 배양액에서 24시간 배양한 후 serum free 배지로 단계적으로 희석한 유자 및 배 식물캘러스 상등액 및 추출액 혹은 유전자 재조합 인간 형질전환성장인자 (recombinant human transforming growth factor-beta, rhTGF-β)를 각 well에 100 μL씩 가하고 24시간 동안 CO2 incubator에서 배양하였다. 이후 세포 배양액을 취해 Procollagen type I C-peptide EIA kit (Takara, Japan)을 이용하여 콜라겐 생합성 양을 측정하였다. 먼저, anti-POD conjugate solution을 100 μL씩 각 well에 첨가하고 시료 혹은 표준액을 20 μL씩 넣은 후 교반한 다음 37oC에서 3시간 반응시켰다. 이후 내용물을 제거하고 인산염완충액 (pH 7.4) 400 μL로 4회 세척하였다. 세척 후 각 well 당 기질 용액을 100 μL씩 넣고 20~30oC에서 15분간 반응시킨 뒤 ELISA stop solution을 100 μL씩 첨가하여 발색을 중지시킨 후 교반하였다. 450 nm에서 흡광도를 측정한 후 표준농도곡선을 이용하여 콜라겐 생합성 양을 산정하였다.Collagen production promoting activity of the callus supernatant and extract of citron and pear plant developed by the above method was evaluated as follows. Human fibroblast cells (CCD-986sk) were inoculated in 96-well plates at a density of 1 × 10 4 cells / well, cultured in DMEM containing 10% FBS for 24 hours, And 100 μL of recombinant human transforming growth factor-beta (rhTGF-β) were added to each well and cultured in a CO 2 incubator for 24 hours. Then, the cell culture was taken and the amount of collagen biosynthesis was measured using Procollagen type I C-peptide EIA kit (Takara, Japan). First, 100 μL of anti-POD conjugate solution was added to each well, 20 μL of sample or standard solution was added, and the mixture was reacted at 37 ° C. for 3 hours. The contents were then removed and washed four times with 400 μL of phosphate buffer (pH 7.4). After washing, 100 μL of the substrate solution was added to each well, reacted at 20 to 30 ° C for 15 minutes, and 100 μL of ELISA stop solution was added to stop the color development. The absorbance at 450 nm was measured and the amount of collagen biosynthesis was calculated using a standard curve.
그 결과, 세포 내에서 proline hydroxylase와 lysyl hydroxylase의 발현의 증가가 실제 fibroblast의 collagen 함량의 증가로 연결되는 지를 확인하기 위하여 total collagen synthsis의 양을 무처리군 (Negative control, 음성대조군), 레티놀 0.1% 처리군(Positive control, 양성 대조군), 잔탄검 0.1% 처리군, 배 캘러스 추출물 1.0% + 잔탄검 0.1% 혼합 처리군, 유자 캘러스 추출물 1.0% + 잔탄검 0.1% 혼합 처리군, 그리고 배 캘러스 추출물 0.5% + 유자 캘러스 추출물 0.5% + 잔탄검 0.1% 혼합 처리군으로 비교하였다. 그 결과 잔탄검, 유자캘러스 추출물, 배 캘러스 추출물 혼합 처리군에서 매우 우수한 콜라겐 생산 증가 효능을 확인할 수 있었다 (도 3).As a result, the amount of total collagen synthase was negatively treated (negative control), retinol 0.1%, and the concentration of proline hydroxylase and lysyl hydroxylase was increased to increase the collagen content of fibroblast. , 0.1% of xanthan gum extract, 0.1% of xanthan gum extract, 0.1% of xanthan gum extract and 0.1% of xanthan gum extract, and 0.1% of xanthan gum extract and 0.1% of xanthan gum extract. % + Citron extract 0.5% + xanthan gum 0.1%. As a result, it was confirmed that the effect of increasing the production of collagen was remarkably improved in the group treated with xanthan gum, citron extract, and bacillus extract (FIG. 3).
화장료 조성물의 제조Preparation of cosmetic composition
본 발명의 배/유자 캘러스 배양 추출물 및 잔탄검을 유효성분으로 함유하는 화장품으로 영양화장수, 크림, 에센스 등의 유화 제형의 화장품 및 유연화장수 등의 가용화 제형의 화장품을 제조하였다.Cosmetics of emulsified form such as nutritional lotion, cream, essence, etc. and cosmetics of the solubilized form such as softened longevity were prepared from the culture / extract of callus of the present invention and xanthan gum as an active ingredient.
제조예 2-1: 화장수Production Example 2-1: Lotion
다음 처방에 따라 통상의 화장수 제조 방법에 따라 제조하였다.According to the following formulation, it was prepared according to the conventional lotion preparation method.
제조예 2-2: 에센스Production Example 2-2: Essence
다음 처방에 따라 통상의 에센스 제조 방법에 따라 제조하였다.According to the following prescription, it was prepared according to a conventional method for producing essence.
제조예 2-3: 로션Production Example 2-3: Lotion
다음 처방에 따라 통상의 로션 제조 방법에 따라 제조하였다.According to the following formulation, it was prepared according to a conventional lotion preparation method.
제조예 2-4: 크림Production Example 2-4: Cream
다음 처방에 따라 통상의 크림 제조 방법에 따라 제조하였다.According to the following formulation, it was prepared according to a conventional cream production method.
제조예 2-5: 젤Production Example 2-5: Gel
다음 처방에 따라 통상의 젤 제조 방법에 따라 제조하였다.According to the following prescription, it was prepared according to a conventional gel preparation method.
이상으로 본 발명의 내용의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다.While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.
Claims (6)
주름 방지 또는 개선을 위한 유효성분으로써 잔탄검을 포함하는 것을 특징으로 하는 피부 외용제 조성물.
A composition for prevention or improvement of wrinkles containing a pear or a Magnoliales callus culture or an extract thereof,
Wherein the composition contains xanthan gum as an active ingredient for preventing or improving wrinkles.
상기 배 캘러스 배양물 또는 그 추출물 100 중량부에 대하여 잔탄검은 5 내지 20중량부로 함유하는 것을 특징으로 하는 피부 외용제 조성물.
The method according to claim 1,
Wherein the composition contains 5 to 20 parts by weight of xanthan gum to 100 parts by weight of the culture of the callus or extract thereof.
상기 유자 캘러스 배양물 또는 그 추출물과, 잔탄검은 10:0.5 내지 2의 중량비로 함유하는 것을 특징으로 하는 피부 외용제 조성물.
The method according to claim 1,
Wherein the content of the citron callus or the extract thereof is in the range of 10: 0.5 to 2 by weight of black xanthan gum.
상기 배 캘러스 배양물 또는 그 추출물 100 중량부에 대하여, 상기 유자 캘러스 배양물 또는 그 추출물은 90 내지 110중량부, 잔탄검은 5 내지 20중량부 함유하는 것을 특징으로 하는 피부 외용제 조성물.
The method according to claim 1,
Wherein said citrus callus culture or its extract contains 90 to 110 parts by weight and 5 to 20 parts by weight of xanthan gum, based on 100 parts by weight of said culture or said extract.
상기 배 캘러스 배양물의 추출물은 배 캘러스 배양물을 건조하고, 분말화하여 정제수에 혼합시킨 다음, 원심분리하여 얻은 상등액이거나,
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 냉수 추출한 추출물이거나,
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 열수 추출한 추출물이거나, 또는
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 에탄올 추출한 추출물인 것을 특징으로 하는 피부 외용제 조성물.
The method according to claim 1,
The extract of the culture of the callus may be a supernatant obtained by drying the culture of the callus, mixing it with powdered water and mixing with purified water, and then centrifuging,
The culture is dried and powdered, mixed with purified water, and then extracted with cold water,
The culture is dried and pulverized, mixed with purified water and then extracted with hot water, or
The composition for external application for skin according to claim 1, wherein the culture is an extract obtained by drying and pulverizing and mixing with purified water followed by ethanol extraction.
상기 유자 캘러스 배양물의 추출물은 유자 캘러스 배양물을 건조하고, 분말화하여 정제수에 혼합시킨 다음, 원심분리하여 얻은 상등액이거나,
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 냉수 추출한 추출물이거나,
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 열수 추출한 추출물이거나, 또는
배양물을 건조하고 분말화하여 정제수에 혼합시킨 후 에탄올 추출한 추출물인 것을 특징으로 하는 피부 외용제 조성물.
The method according to claim 1,
The extract of the citron callus culture may be a supernatant obtained by drying the citron callus culture, mixing it into powder and mixing with purified water, and then centrifuging,
The culture is dried and powdered, mixed with purified water, and then extracted with cold water,
The culture is dried and pulverized, mixed with purified water and then extracted with hot water, or
The composition for external application for skin according to claim 1, wherein the culture is an extract obtained by drying and pulverizing and mixing with purified water followed by ethanol extraction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020170047598A KR101949937B1 (en) | 2017-04-12 | 2017-04-12 | Anti-aging Composition for Skin External Application Comprising Callus extracts from Citrus junos Siebold ex Tanaka and Pyrus pyrifolia Nakai and Xanthan Gum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020170047598A KR101949937B1 (en) | 2017-04-12 | 2017-04-12 | Anti-aging Composition for Skin External Application Comprising Callus extracts from Citrus junos Siebold ex Tanaka and Pyrus pyrifolia Nakai and Xanthan Gum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20180115397A true KR20180115397A (en) | 2018-10-23 |
| KR101949937B1 KR101949937B1 (en) | 2019-02-22 |
Family
ID=64101889
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020170047598A KR101949937B1 (en) | 2017-04-12 | 2017-04-12 | Anti-aging Composition for Skin External Application Comprising Callus extracts from Citrus junos Siebold ex Tanaka and Pyrus pyrifolia Nakai and Xanthan Gum |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR101949937B1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20240083701A (en) | 2022-12-05 | 2024-06-12 | 배장필 | Anti-odor composition |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001158728A (en) * | 1999-12-01 | 2001-06-12 | Shiseido Co Ltd | Agent for promoting production of hyaluronic acid and skin preparation for external use |
| KR20050041259A (en) * | 2003-10-30 | 2005-05-04 | 윤성용 | Use of a callus for development of natural cosmetics |
| KR20090013293A (en) * | 2007-08-01 | 2009-02-05 | 안성마춤농협조합공동사업법인 | Anti-wrinkle cosmetic composition containing pear extract |
| KR20120119797A (en) * | 2011-04-22 | 2012-10-31 | (주) 마마치 | Cosmetic composition that is effective against wrinkles |
| KR20150081201A (en) | 2014-01-03 | 2015-07-13 | 공감스토리 주식회사 | Cosmetic composition for whitening and manufacturing method thereof |
| KR20150139688A (en) | 2014-06-03 | 2015-12-14 | 조정희 | The Extract Of The Citrus grandis Osbeck Having Skin Whitening Activity And Cosmetic Composition Containing The Same |
-
2017
- 2017-04-12 KR KR1020170047598A patent/KR101949937B1/en active IP Right Grant
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001158728A (en) * | 1999-12-01 | 2001-06-12 | Shiseido Co Ltd | Agent for promoting production of hyaluronic acid and skin preparation for external use |
| KR20050041259A (en) * | 2003-10-30 | 2005-05-04 | 윤성용 | Use of a callus for development of natural cosmetics |
| KR20090013293A (en) * | 2007-08-01 | 2009-02-05 | 안성마춤농협조합공동사업법인 | Anti-wrinkle cosmetic composition containing pear extract |
| KR20120119797A (en) * | 2011-04-22 | 2012-10-31 | (주) 마마치 | Cosmetic composition that is effective against wrinkles |
| KR20150081201A (en) | 2014-01-03 | 2015-07-13 | 공감스토리 주식회사 | Cosmetic composition for whitening and manufacturing method thereof |
| KR20150139688A (en) | 2014-06-03 | 2015-12-14 | 조정희 | The Extract Of The Citrus grandis Osbeck Having Skin Whitening Activity And Cosmetic Composition Containing The Same |
Also Published As
| Publication number | Publication date |
|---|---|
| KR101949937B1 (en) | 2019-02-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101914157B1 (en) | Cosmetic composition comprising camellia sinensis constituents | |
| JP3415198B2 (en) | External preparation for skin | |
| KR101502687B1 (en) | Anti-aging and Anti-inflammatory and Anti-oxidant Cosmetic Composition including Beans Placenta Cell Cultures Extracts | |
| KR101838354B1 (en) | Anti-aging composition for skin external application comprising Camellia japonica Plant Cell Culture Extract and Method for Preparing the Same | |
| KR20150064515A (en) | Anti-aging Composition for Skin External Application Comprising Magnoliale Placenta Culture Extracts | |
| KR102016062B1 (en) | Callus extracts from Citrus junos Siebold ex Tanaka and Pyrus pyrifolia Nakai with Whitening and Anti-wrinkle effect | |
| JP3619185B2 (en) | Cosmetics | |
| KR101305698B1 (en) | Skin External Composition Containing Myrothamnus flabellifolia Callus Extract | |
| KR101949937B1 (en) | Anti-aging Composition for Skin External Application Comprising Callus extracts from Citrus junos Siebold ex Tanaka and Pyrus pyrifolia Nakai and Xanthan Gum | |
| KR101467033B1 (en) | Composition for Skin External Application Comprising Culture Extract of Soy Bean Placenta | |
| JP2006290829A (en) | Basement membrane stabilizer | |
| KR102441009B1 (en) | Methods for extracting compound from ginseng, ginseng extract comprising the compound and composition for enhancing skin barrier comprising the same | |
| KR102326086B1 (en) | Composition for Improving Skin Conditions Having Skin Whitening, Moisturizing, Anti-Wrinkle and Skin Barrier Property Comprising Complex Extracts of Broussonetia kazinoki as Active Ingredient | |
| KR101619710B1 (en) | Anti-aging Composition for Skin External Application Comprising Liriodendron tulipifera Placenta Culture Extracts | |
| KR101427027B1 (en) | A Skin External Composition Containing Callus Extract Derived from Chaenomeles Sinensis | |
| KR20230119344A (en) | Methods for manufacturing angelica gigas nakai extract using fermentation bacteria | |
| KR102157395B1 (en) | Cosmetic Composition including Rosa davurica Pall Callus Cultured in medicum containing mineral water | |
| KR20220141191A (en) | Composition for Skin Whitening, Moisturizing, Strengthening Skin Barrier, Skin Cell Regeneration, and Anti-Wrinkle Property Comprising Fermented Extract of Broussonetia kazinoki as Active Ingredient | |
| KR102121457B1 (en) | Cosmetic composition comprising the extract of fermented Sophora Japonica Fruits for skin anti-wrinkle effect and producing method thereof | |
| KR102605467B1 (en) | Cosmetic composition for skin improvement comprising an extract of alfalfa seed-derived glycoprotein | |
| KR101619711B1 (en) | Anti-aging Composition for Skin External Application Comprising Schizandra chinensis Placenta Culture Extracts | |
| KR102716045B1 (en) | Cosmetic composition using amino acid derivatives | |
| KR102643767B1 (en) | A cosmetic composition for improving skin barrier function containing enzyme treated molochia as an active ingredient | |
| KR102606380B1 (en) | Composition for enhancing skin barrier containing Bolboschoenus planiculmis extract | |
| KR20190047629A (en) | Skincare composition comprising safflower Ferment Extract fermented by Phellinus linteus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| PA0109 | Patent application |
Patent event code: PA01091R01D Comment text: Patent Application Patent event date: 20170412 |
|
| PA0201 | Request for examination | ||
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20180326 Patent event code: PE09021S01D |
|
| PG1501 | Laying open of application | ||
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 20181112 |
|
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 20190213 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 20190214 End annual number: 3 Start annual number: 1 |
|
| PG1601 | Publication of registration | ||
| PR1001 | Payment of annual fee |
Payment date: 20220303 Start annual number: 4 End annual number: 4 |
|
| PR1001 | Payment of annual fee |
Payment date: 20230131 Start annual number: 5 End annual number: 5 |
|
| PR1001 | Payment of annual fee |
Payment date: 20240104 Start annual number: 6 End annual number: 6 |
|
| PR1001 | Payment of annual fee |
Payment date: 20241224 Start annual number: 7 End annual number: 7 |