KR20180105361A - Drink composition comprising a Black Galic and a Red Ginseng and method of making the same - Google Patents
Drink composition comprising a Black Galic and a Red Ginseng and method of making the same Download PDFInfo
- Publication number
- KR20180105361A KR20180105361A KR1020170032339A KR20170032339A KR20180105361A KR 20180105361 A KR20180105361 A KR 20180105361A KR 1020170032339 A KR1020170032339 A KR 1020170032339A KR 20170032339 A KR20170032339 A KR 20170032339A KR 20180105361 A KR20180105361 A KR 20180105361A
- Authority
- KR
- South Korea
- Prior art keywords
- concentrate
- red ginseng
- ginseng powder
- black garlic
- black
- Prior art date
Links
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Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/01—Instant products; Powders; Flakes; Granules
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
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- B01F15/02—
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
- B01F27/808—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with stirrers driven from the bottom of the receptacle
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/75—Discharge mechanisms
- B01F35/754—Discharge mechanisms characterised by the means for discharging the components from the mixer
- B01F35/75425—Discharge mechanisms characterised by the means for discharging the components from the mixer using pistons or plungers
- B01F35/754251—Discharge mechanisms characterised by the means for discharging the components from the mixer using pistons or plungers reciprocating in the mixing receptacle
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
Description
본 발명은 흑마늘 농축액과 홍삼분말을 포함하는 고점도 음료조성물 및 이의 제조방법에 관한 것으로, 보다 상세하게는 흑마늘 농축액과 홍삼분말을 이용하여 별도의 점증제를 사용하지 않고도 적절한 점성을 가지는 고점도 음료 조성물 및 이의 제조방법에 관한 것이다. The present invention relates to a high viscosity beverage composition comprising a black garlic concentrate and red ginseng powder and a method for preparing the same, and more particularly, to a high viscosity beverage composition having a proper viscosity without using a thickening agent using a black garlic concentrate and red ginseng powder, And a method for producing the same.
흑마늘은 생마늘을 40~100℃에서 온도를 변화시키면서 10~40일간 숙성시켜 만드는 마늘 가공품으로 숙성되는 동안 마늘 자체에 함유되어 있던 당과 아미노산이 반응하여 갈변물질을 생성함으로써 마늘의 내부까지 흑색으로 변화되어 생마늘의 흰 빛과 대조되는 개념으로 흑마늘로 불리게 되었다. 흑마늘로 변화되는 과정에서 생마늘 특유의 매운 맛과 향이 감소되고, 딱딱한 조직이 연화되어 젤리와 같은 식감을 가지며, 열처리 과정 중에 유기산이나 당이 유리되어 새콤달콤한 맛을 가지게 된다. Black garlic is a processed garlic product made by aging raw garlic at 40 ~ 100 ℃ for 10 ~ 40 days while changing temperature. It reacts with sugar and amino acid contained in garlic itself to produce browning substance, It is called the black garlic as a concept that contrasts with the white light of raw garlic. In the course of changing to black garlic, the pungent flavor and aroma unique to raw garlic are reduced, the hard tissue is softened, and it has a jelly-like texture and organic acids and sugars are liberated during the heat treatment process, resulting in a sweet and sour taste.
생마늘에 있던 매운 맛과 향을 내는 주체 물질인 황화합물이 제거되고, 페놀화합물이나 생마늘에는 소량 존재하던 S-allylcystein(SAC)의 함량은 증가된다. 페놀화합물은 식물성 식품에서 함유되어 있는 대표적인 기능성 성분으로 페놀기를 함유하는 화합물을 총칭하는데, 이들 화합물은 우수한 항산화 기능성을 가지고 있다. SAC는 유용물질과 갈변물질의 함량 증가로 생마늘보다 높은 항산화활성을 가지며, 혈액응고 억제, 혈중 중성지질 및 콜레스테롤 저하기능, 면역조절 기능 및 항암활성을 가지고 있다. 이러한 기능성으로 인해 체력 및 활력 보강, 피로회복, 면역강화, 혈중 지질농도 개선의 활성이 있는 식품으로 인지되고 있다. Sodium alginate (SAC), which is a minor substance in phenolic compounds and raw garlic, is removed. Phenol compounds are representative functional ingredients contained in plant foods, and collectively referred to as compounds containing phenol groups, and these compounds have excellent antioxidant functionality. SAC has higher antioxidant activity than raw garlic due to increased content of useful substances and browning substances, and has blood coagulation inhibition, blood lipid and cholesterol lowering function, immunoregulatory function and anticancer activity. Because of these functionalities, it is recognized as a food having activity of strengthening physical strength and vitality, restoring fatigue, strengthening immunity, and improving blood lipid concentration.
인삼은 예로부터 신체 허약을 회복시키고, 체내의 독소를 제거하며, 병에 대한 저항력을 증진시키는 것으로 알려져 있고, 여러 연구를 통해 피로회복, 스트레스 해소, 동맥경화증 예방, 혈압과 혈당 저하, 노화방지 및 성기능 회복의 기능성을 가지는 것으로 알려져 있다. 수삼을 쪄서 말리는 과정을 반복하여 얻어지는 홍삼은 배당체와 사포닌, 플라보노이드계화합물 등이 함유되어 있어 중추신경 진정 및 흥분작용, 조혈작용 및 간 보호, 항염, 항종양작용이 있어 건강기능성 식품 소재로 널리 이용되고 있다. Ginseng has been known to restore body weakness, eliminate toxins in the body, and increase resistance to diseases. Various studies have been conducted to find out the effects of ginseng on fatigue, stress relief, prevention of arteriosclerosis, blood pressure and hypoglycemia, It is known to have the functionality of sexual recovery. Red ginseng, obtained by repeating the process of steamed and dried ginseng, contains glycosides, saponins, and flavonoid compounds, and is widely used as a health functional food material because of its central nervous sedation and excitability, hematopoiesis and liver protection, anti-inflammation and anti- .
흑마늘의 경우 외피를 제거하지 않은 톨마늘 형태로 판매, 유통되거나 깐마늘 형태로 200~500 g 단위로 판매되고 있어 보다 용이하게 섭취하기 위한 방법으로 물과 함께 추출하여 음료로 이용되고 있다. 이 경우 8~15 brix 정도의 농도를 가지며, 80~100 mL 단위로 포장되고 있다. 홍삼의 경우는 수분이 15% 미만으로 건조되므로 직접 섭취에는 어려움이 있어 분말로 가공하거나 역시 물과 함께 추출하여 음료로 유통되고 있다. In the case of black garlic, it is sold in to garlic form which does not remove the outer coat, and it is sold in circulation or garlic form in 200 ~ 500 g unit, so it is used as a beverage by extracting it with water as a method for easier ingestion. In this case, it has a concentration of 8 ~ 15 brix and is packed in 80 ~ 100 mL unit. In case of red ginseng, the water is dried to less than 15%, so it is difficult to directly ingest, so it is processed into powder or extracted with water and distributed as a drink.
흑마늘과 홍삼 모두 추출액을 고농도로 농축한 제품이 개발되어 있으나 고가이며, 꿀과 유사한 점성을 가지므로 섭취시마다 떠서 먹기가 용이하지 않은 단점이 있다. 이러한 기존의 포장 방법들은 휴대하기에 부피가 크거나 덜어서 휴대하기가 용이하지 않아 꾸준히 섭취하여야 효과를 얻을 수 있는 건강 증진 또는 예방용 식품의 특성을 고려할 때 지속적인 섭취에 대한 한계를 가지고 있다. Although both black garlic and red ginseng have been developed to concentrate the extract at a high concentration, it is expensive and has a viscosity similar to that of honey, so it has drawbacks in that it is not easy to eat each time it is consumed. These conventional packaging methods have limitations on continuous ingestion considering the characteristics of health promotion or preventive food which can be effectively consumed because it is bulky or loose to carry and is not easy to carry.
이와 유사한 관점에서 공개특허 제10-2016-0018999호에서는 예방학적 차원에서 식품을 섭취함에 있어 적정량을 장기간 섭취하는 데는 어려움이 있으며, 특히 마늘의 경우 기능성은 높지만 특유의 향취와 맛, 소화기관에 대한 자극성이 있으므로 이러한 단점을 보완하기 위하여 우유, 두유와 같은 유제품에 흑마늘진액 0.1~20중량부와 홍삼 또는 인삼 진액 0.001~10중량부 및 홍삼으로부터 분리한 당지질단백질인 진토닌을 0.001~10중량부 중 선택된 하나 이상의 성분을 포함하는 흑마늘 함유 홍삼 유음료의 제조방법을 제안하고 있다. 하지만 이 경우 흑마늘이나 홍삼의 첨가량이 상대적으로 낮고, 유제품은 품질의 유지 및 관리를 위하여 저온유통 하여야 하며, 액상으로 부피가 크고, 일반 식품에 비해 유통기한이 짧은 단점이 있어 이를 개선할 필요가 있다. In a similar viewpoint, in the patent application 10-2016-0018999, it is difficult to take a proper amount of food for a long period of time for the ingestion of food at the preventive level. Especially, the garlic has a high functionality but has a unique flavor and taste, In order to compensate for these disadvantages, 0.1 to 20 parts by weight of black ginseng extract, 0.001 to 10 parts by weight of red ginseng or ginseng essence, and 0.001 to 10 parts by weight of ginseng protein, which is a glycolipid protein isolated from red ginseng, are added to dairy products such as milk and soy milk A method for manufacturing a black gum containing red ginseng milk beverage containing at least one selected ingredient is proposed. However, in this case, the addition amount of black ginseng or red ginseng is relatively low, and dairy products must be circulated at low temperatures in order to maintain and manage quality, and it is bulky in liquid phase and has a short shelf life compared to general foods. .
홍삼과 흑마늘의 기능성과 맛이 조화된 음료 조성물을 제조하기 위한 공개특허 제10-2012-0019988호에서는 사포닌 성분의 체내 흡수율 및 체내 활성을 개선시킨 흑마늘과 발효홍삼을 포함하는 음료를 제조하고, 장기보관을 위해 살균, 냉각하는 단계를 포함하는 음료의 제조법을 제안하고 있다. 이 특허의 경우에서는 흑마늘 농축물이나 발효홍삼 농축물을 10 중량% 이하로 비교적 낮은 비율로 함유하고 있고, 점증제와 감미제, 보존료, 산미료 등의 식품첨가물을 다량 첨가하는 한계를 가지고 있다. In Patent Publication No. 10-2012-0019988 for preparing a beverage composition in which the functionality and taste of red ginseng and black garlic are harmonized, a beverage containing black garlic and fermented red gins improved in water absorption and body activity of saponin component was prepared, And a step of sterilizing and cooling for storage. In the case of this patent, the black gum concentrate or fermented red ginseng concentrate is contained at a relatively low ratio of 10% by weight or less, and it has a limitation to add a large amount of food additives such as thickening agent, sweetener, preservative, and acidulant.
상기한 문제를 해결하고자 하는 본 발명은 흑마늘 농축액과 홍삼 유효성분의 함량이 높은 미세한 홍삼분말을 배합하여 소량 섭취로 다량 섭취시와 동일한 효능을 얻을 수 있도록 하며, 별도의 첨가물 첨가 없이 물성을 개선하여 적절한 점성을 가지는 흑마늘 농축액과 홍삼분말을 포함하는 고점도 음료의 조성물 및 이의 제조방법을 제공하는 것을 목적으로 한다.In order to solve the above problem, the present invention is to solve the above-mentioned problems by mixing a black gum concentrate and a fine red ginseng powder having a high content of red ginseng active ingredient so as to obtain the same effect as when ingested in a small amount, It is an object of the present invention to provide a composition of a high viscosity beverage containing a black gum concentrate and red ginseng powder having an appropriate viscosity and a method for producing the same.
또한, 배 농축액, 도라지 농축액을 추가하여 기능성을 높인 흑마늘 농축액과 홍삼분말을 포함하는 고점도 음료의 조성물 및 이의 제조방법을 제공하는 것을 목적으로 한다. It is another object of the present invention to provide a composition of a highly viscous beverage containing a black gum concentrate and red ginseng powder having enhanced functionality by adding a brewing concentrate and a bellflower concentrate, and a process for producing the same.
본 발명은 흑마늘을 물 추출한 후 30~60 brix로 농축한 흑마늘 농축액에 800 mesh 미세 홍삼분말을 흑마늘 농축액 무게대비 5~7% 혼합한 것을 포함하는 특징으로 한 흑마늘 농축액과 홍삼분말을 포함하는 고점도 음료조성물을 제공한다. The present invention relates to a method for producing high-viscosity beverages containing black garlic concentrate and red ginseng powder, which comprises mixing a black garlic concentrate having a concentration of 30 to 60 brix after extracting water from black garlic with 800 mesh micro ginseng powder in an amount of 5 to 7% Lt; / RTI >
본 발명은 30~60 brix 흑마늘 농축액 20~40 중량%, 미세 홍삼분말 3~8 중량%, 68~75 brix 배 농축액 9~19 중량% 및 45~53 brix 도라지 농축액 0.5~1.5 중량%, 정제수 35~65 중량%로 이루어지는 흑마늘 농축액과 홍삼분말을 포함하는 고점도 음료조성물을 제공한다.The present invention relates to a pharmaceutical composition comprising 30 to 60 brix of
본 발명은 30~60 brix 흑마늘 농축액, 미세 홍삼분말, 68~75 brix 배 농축액, 도라지 농축액을 교반기에 넣어서 90℃에서 30분간 교반하면서 혼합하는 것을 포함하는 흑마늘 농축액과 홍삼분말을 포함하는 고점도 음료의 제조방법을 제공한다. The present invention relates to a high viscosity beverage containing black garlic concentrate and red ginseng powder, which comprises mixing 30 to 60 brix of black garlic concentrate, micro ginseng powder, 68 to 75 brix concentrate, and bellflower concentrate in a stirrer while stirring at 90 ° C for 30 minutes And a manufacturing method thereof.
상기 교반기는 교반조와 상기 교반조 내부로 위치되며 하단에 배출공이 형성된 실린더; 상기 실린더 내벽에 밀착되는 말착부를 구비한 피스톤; 모터의 회전력으로 회전되는 회전축의 끝단에 부착되어 교반작동하는 교반날개를 포함하여 구성된 교반장치로 이루어지는 것이 바람직하다. Wherein the agitator comprises a stirrer, a cylinder positioned inside the agitator, and a discharge hole formed at a lower end thereof; A piston having an anchor portion which is in close contact with the inner wall of the cylinder; And an agitating blade attached to an end of a rotating shaft rotated by the rotational force of the motor and stirring the agitating blade.
본 발명에 의해 제조된 음료는 액상스틱으로 포장하기에 적합하며, 고농도의 성분들이 혼합되어 흑마늘과 홍삼의 유효성분들을 모두 함유하고 있고, 검성분이나 점증제 등을 혼합하지 않고도 고점도를 가지므로 별도의 첨가물 없이 천연소재만으로 제품에 원하는 물성을 제공할 수 있는 장점이 있다. The beverage prepared according to the present invention is suitable for packing in a liquid stick, and contains high-concentration components and contains both effective components of black ginseng and red ginseng. Since it has a high viscosity without mixing gum components or thickeners, It is possible to provide desired physical properties to the product by using only natural materials without additives.
도 1은 미세 홍삼분말 혼합물의 혼합비율을 달리한 흑마늘 농축액 혼합물의 관능평가를 나타낸 것이다.
도 2는 도라지와 배 추출물의 RAW 264.7 세포에 대한 세포독성을 나타낸 것이다.
도 3은 도라지와 배 추출물의 RAW 264.7 세포에서 NO 생성율을 나타낸 것이다.
도 4는 도라지와 배 추출물의 RAW 264.7 세포에서 ROS 생성율을 나타낸 것이다.
도 5는 흑마늘 농축액, 홍삼 분말 및 배와 도라지 농축액을 함유하는 고농축 음료의 관능평가를 나타낸 것이다.
도 6(가),(나) 및(다)는 본 발명의 바람직한 일실시예에 따른 교반장치의 작동 상태도를 나타낸 것이다.Fig. 1 shows the sensory evaluation of a mixture of black gum concentrate with different mixing ratios of the micro ginseng powder mixture.
Figure 2 shows the cytotoxicity of bellflower roots and embryo extracts to RAW 264.7 cells.
Fig. 3 shows the NO production rate in RAW 264.7 cells of Platycodon grandiflorum and the pear extract.
Fig. 4 shows the ROS production rate in RAW 264.7 cells of Platycodon grandiflorum and Pigeon Extract.
Fig. 5 shows the sensory evaluation of highly concentrated beverages containing black garlic concentrate, red ginseng powder and pear and bellflower concentrate.
6 (a), 6 (b) and 6 (c) show operation states of an agitation apparatus according to a preferred embodiment of the present invention.
본 발명에서 제공하고자 하는 흑마늘 농축액과 홍삼분말을 포함하는 고점도 음료조성물 및 이의 제조방법에 대해 이하 실시예 및 실험예를 통하여 더욱 상세히 설명하고자 한다. 실시예와 실험예는 본 발명의 요지를 설명하기 위한 것으로 이에 따라 본 발명의 범위가 한정되는 것은 아니다.The high viscosity beverage composition comprising the black gum concentrate and the red ginseng powder to be provided in the present invention and the preparation method thereof will be described in more detail with reference to the following examples and experimental examples. The examples and experimental examples are intended to illustrate the gist of the present invention and thus the scope of the present invention is not limited thereto.
실시예Example 1 : One : 흑마늘Black garlic 농축액과 홍삼분말 혼합 고점도 음료제조를 위한 적정농도 설정 Mixing of concentrated and red ginseng powder Proper concentration setting for high viscosity beverage manufacturing
고농축 흑마늘 제품의 제조에 적합한 농도를 설정하기 위하여 흑마늘 추출액을 농축하여 각각 20, 30, 40, 50, 60 및 70 brix로 조절한 후 농축액의 무게 대비 5%에 해당하는 홍삼 분말을 혼합하였다. 이때 홍삼분말의 입도는 100±10 mesh (일반분말)와 800±10 mesh (미세분말)로 상이한 2종을 사용하였으며, 홍삼분말과 흑마늘 농축액을 혼합한 다음 상온에서 30분간 방치하여 홍삼분말이 흑마늘 농축액 중으로 완전히 퍼져 균질화되도록 한 다음 다시 교반하였다. 교반된 혼합 농축물이 음용에 적절한 점성을 가지는 지를 확인하기 위하여 20±3℃의 상온에서 보관하면서 점도를 측정하였으며, 농도별 흑마늘 농축액의 총 페놀화합물 함량을 분석하였다. The black garlic extracts were concentrated to 20, 30, 40, 50, 60 and 70 brix, respectively, and the red ginseng powder corresponding to 5% of the weight of the concentrate was mixed. The red ginseng powder and the black garlic concentrate were mixed and the mixture was allowed to stand at room temperature for 30 minutes. The red ginseng powder was mixed with black garlic (100 g) It was thoroughly spread into the concentrate to homogenize and then stirred again. To determine whether the mixed concentrates were suitable for drinking, the viscosity was measured at room temperature of 20 ± 3 ° C, and the content of total phenolic compounds in the concentrated black gum was analyzed by concentration.
실험예Experimental Example 1 : 점도 측정 1: Viscosity measurement
흑마늘 농축액의 농도와 홍삼 분말의 입자 크기를 달리하여 제조한 혼합 농축액의 점도는 디지털 점도계를 이용하여 측정하였다. 점도계의 L3 스핀들을 사용하였으며, 3분간 100 rpm의 속도로 교반하다가 1분이 지난 후부터 30초 간격으로 총 5회 점도를 측정한 값을 평균하여 해당 시료의 점도로 하였으며, 각각의 시료는 3회 반복 측정하였다. 이 때 흑마늘 농축액의 점도를 비교하기 위한 대상으로 동서벌꿀을 시료로 하여 동일한 조건에서 점도를 측정하였으며, 이 때 꿀의 무게 대비 물을 10~50 %까지 첨가하여 비교데이터를 확보하였다. The viscosity of the mixed concentrate prepared by varying the concentration of black garlic concentrate and the particle size of red ginseng powder was measured using a digital viscometer. The L3 spindle of the viscometer was used. The sample was stirred for 3 minutes at a speed of 100 rpm, and then the viscosity of the sample was measured by averaging the values obtained by measuring the viscosity five times at intervals of 30 seconds after 1 minute. Each sample was repeated three times Respectively. To compare the viscosity of black garlic concentrate, the viscosities were measured under the same conditions using East-West honey as a sample, and comparative data were obtained by adding 10 ~ 50% of water to the weight of honey.
농도별 흑마늘 농축액에 일반분말 및 미세분말을 각각 동일한 양으로 혼합한 후 보관 온도를 달리하여 저장하여 점도를 측정한 결과는 표 1과 같다. 흑마늘 농축액의 점도는 농도와 비례하여 높아졌으며 동일한 농도의 홍삼분말을 사용하였으나 일반 홍삼분말을 사용하였을 때보다 미세분말을 사용하였을 때 점도가 더 높았다. 즉, 50 brix의 흑마늘 농축액에 홍삼 일반분말을 첨가하였을 때 점도는 34.96 cPs 였는데, 미세분말을 첨가하였을 때는 30 brix 흑마늘 농축액에서 32.14 cPs로 유사한 범위의 점도를 나타내었다. 또한 흑마늘 농축액 60 brix를 사용하였을 때에 비하여 70 brix를 사용하였을 때 점도는 흑마늘 농축액 자체의 점도도 약 6.2배 더 증가하였으며, 일반분말 첨가시 약 4.6배, 미세 홍삼분말을 첨가하였을 때는 약 4.5배 더 점도가 증가하였다. Table 1 shows the results of measuring the viscosity by storing the same amount of common powder and fine powder in concentrated black gum concentrate at different storage temperatures. The viscosity of black garlic concentrate increased in proportion to the concentration and the same concentration of red ginseng powder was used. However, the viscosity of black ginseng powder was higher when using fine powder than that of normal red ginseng powder. That is, when the red ginseng powder of 50 brix was added to the red ginseng powder, the viscosity was 34.96 cPs. When the fine powder was added, the viscosity of the 30 brix black germ extract showed a similar range of 32.14 cPs. When 70 brix was used, the viscosity of the black garlic concentrate was increased about 6.2 times. The addition of normal powder and the addition of micro ginseng powder resulted in a 4.5 times increase Viscosity increased.
구분sample
division
A : 홍삼 분말을 첨가하지 않은 농도별 흑마늘 농축액 A: Concentrated black garlic concentrate with no added red ginseng powder
B : 농도별 흑마늘 농축액에 홍삼 일반분말을 5% 첨가한 시료 B: Samples in which 5% of red ginseng powder was added to black gum concentrate by concentration
C : 농도별 흑마늘 농축액에 홍삼 미세분말을 5% 첨가한 시료 C: A sample in which 5% of red ginseng fine powder was added to concentrated black garlic concentrate
점도에 대한 비교확인을 위하여 꿀에 무게대비 일정 비율의 물을 가하여 충분히 혼합한 후 점도를 측정한 결과는 표 2와 같다. 상기 표 1의 분석결과와 비교해 볼 때 40 brix의 흑마늘 농축액과 여기에 홍삼 일반분말을 혼합한 시료는 꿀과 물을 1:1로 혼합한 정도의 점성을 가졌으며, 홍삼 미세분말을 혼합하였을 때는 20 brix의 흑마늘 농축액과 혼합하였을 때도 30.08 cPs의 높은 점도를 가져 꿀과 물을 6:4의 비율로 혼합하였을 때와 유사한 점도를 나타내었다. For comparison of viscosities, a certain amount of water was added to honey and the mixture was thoroughly mixed. Viscosity was measured and the results are shown in Table 2. Compared with the results of Table 1, 40 brix of black garlic concentrate and red ginseng powder were mixed with honey and water at a ratio of 1: 1, and when red ginseng powder was mixed, 20 brix of black garlic concentrate showed high viscosity of 30.08 cPs, similar to that of honey and water mixed at a ratio of 6: 4.
이처럼 흑마늘 농축액을 단독으로 사용할 때에 비해 홍삼 분말이 첨가됨으로써 점도는 상승하며, 분말의 입자가 고울수록 점도를 더 상승시키는 효과가 있어 홍삼 일반분말보다는 미세분말을 사용하는 것이 고점도의 음료를 제조하는 데 유리함을 알 수 있다. 하지만 70 brix의 흑마늘 농축액에 미세분말을 첨가하였을 때는 높은 점성을 가지므로 음료의 점성으로는 부적합하였다. Thus, when the black gum concentrate alone is used, the viscosity is increased by adding the red ginseng powder, and the higher the particle size of the powder is, the more the viscosity is increased. Therefore, the use of the fine powder rather than the red ginseng general powder The advantage can be seen. However, when the fine powder was added to the black bell pepper concentrate of 70 brix, it was not suitable for beverage viscosity because it had high viscosity.
실험예 2 : 총 폴리페놀 화합물의 함량 측정 Experimental Example 2: Determination of total polyphenol compound content
폴리 페놀성 화합물은 식물계에 널리 분포되어 있는 2차 대사 산물의 하나로 다양한 구조와 분자량은 가지며 이는 다양한 생리적 기능을 나타내는 것으로 보고된 바가 있다. 가장 대표적으로는 항산화활성은 페놀화합물의 함량과 비례하는 것으로 알려져 있다. 따라서 본 발명에서는 대표적인 기능성 성분으로 총 페놀화합물의 함량을 분석하였다. Polyphenolic compounds are one of the secondary metabolites widely distributed in the vegetable field, and have various structures and molecular weights, which have been reported to exhibit various physiological functions. Antioxidant activity is known to be proportional to the content of phenolic compounds. Therefore, in the present invention, the content of total phenol compounds was analyzed as a representative functional ingredient.
총 폴리페놀 화합물의 함량은 폴리페놀성 물질인 phosphomolybdic acid와 반응하여 청색을 나타내는 원리로 Folin-Denis법에 따라 측정하였다. 즉, 30 brix 흑마늘 농축액을 이용해 호화 온도와 시간을 달리하여 제조한 혼합농축액 각각에 증류수를 가하여 100배 희석한 것을 시료액을 사용하였다. 시료액 1 mL에 2 M Folin-Ciocalteau 시약(Sigma Co., St. Louis, MO, USA) 0.5 mL를 넣고 3분 후 10% Na2CO3 용액(Daejung, Siheung, Korea) 0.5 mL씩을 혼합하여 실온의 암실에서 1시간 정치한 다음 분광광도계(Libra S 35, Biochrom, Cambridge, England)를 이용하여 760 nm에서 흡광도를 측정하였다. 표준물질로는 gallic acid(Sigma Co., St. Louis, MO, USA)를 사용하여 시료와 동일한 방법으로 분석하여 얻은 검량선으로부터 총 페놀화합물의 함량을 계산하였다. The total polyphenol compound content was measured by the Folin-Denis method as a principle of reacting with a polyphenolic substance, phosphomolybdic acid, to show blue color. That is, each of the mixed concentrates prepared by varying the gelatinization temperature and time using 30 brix black garlic concentrate was diluted 100 times with distilled water, and the sample solution was used. 0.5 mL of 2 M Folin-Ciocalteau reagent (Sigma Co., St. Louis, MO, USA) was added to 1 mL of the sample solution, and after 3 minutes, 0.5 mL of 10% Na 2 CO 3 solution (Daejung, Siheung, After standing for 1 hour in a dark room at room temperature, the absorbance was measured at 760 nm using a spectrophotometer (Libra S 35, Biochrom, Cambridge, England). The content of total phenolic compounds was calculated from the calibration curve obtained by using gallic acid (Sigma Co., St. Louis, Mo., USA) as a reference material.
구분sample
division
A : 홍삼 분말을 첨가하지 않은 농도별 흑마늘 농축액 A: Concentrated black garlic concentrate with no added red ginseng powder
B : 농도별 흑마늘 농축액에 홍삼 일반분말을 5% 첨가한 시료 B: Samples in which 5% of red ginseng powder was added to black gum concentrate by concentration
C : 농도별 흑마늘 농축액에 홍삼 미세분말을 5% 첨가한 시료 C: A sample in which 5% of red ginseng fine powder was added to concentrated black garlic concentrate
홍삼 분말을 첨가하지 않은 흑마늘 농축액에 비해 홍삼분말을 첨가하였을 때, 그리고, 일반분말보다는 미세분말을 혼합하였을 때 총 페놀화합물의 함량은 더 증가하였다. 홍삼 미세분말은 일반 분말에 비하여 더 입자가 적고, 분쇄하는 동안이 세포막이 파괴되어 섭취시 체내에서 흡수력이 더 높은 특징을 가지므로 홍삼이 가지는 기능성 성분의 흡수율을 최대로 높일 수 있다. The content of total phenolic compounds was increased when red ginseng powder was added and fine powder was mixed than general powder compared to black ginseng concentrate without red ginseng powder. Since red ginseng powder has smaller particle size than general powder and has a high absorption capacity in body during ingestion of the cell membrane during pulverization, the absorption rate of functional ingredient of red ginseng can be maximized.
이상의 분석 결과를 종합하여 볼 때 흑마늘 농축액에 첨가하는 홍삼분말은 일반분말보다는 미세분말을 사용하는 것이 더 적절하며, 이들을 혼합할 때 점성이나 유효성분인 페놀화합물의 함량을 고려할 때 흑마늘 농축액은 60 brix 이하의 것을 사용하는 것이 적절하였다. Based on the above results, it is more appropriate to use the fine powder than the ordinary powder in the black gum concentrate. Considering the viscosity and the content of the phenol compound as the active ingredient, the concentration of the black garlic concentrate is 60 brix It was appropriate to use the following.
실시예 2 : 흑마늘 농축액과 미세홍삼 분말의 적정 혼합 비율 설정Example 2: Setting the proper mixing ratio of black gum concentrate and micro ginseng powder
흑마늘 농축액과 미세홍삼 분말을 혼합한 농축음료 제조를 위한 조건 설정의 일환으로 미세홍삼 분말의 적정 농도를 설정하기 위하여 상기 실시예 1에 따라 제조한 시료의 점도와 흐름성 분석 결과에 기초하여 흑마늘 농축액은 60 brix를 사용하되, 여타 부재료가 더 첨가될 경우를 고려하여 농축액을 활용한 음료 제조시 최저 농도로 설정한 30 brix로 조정한 것을 동시에 사용하여 비교하였다. 관능적 특성을 중심으로 예비 평가를 실시한 결과 홍삼 미세 분말은 10% 이내로 사용하는 것이 적절하였기에 일정량의 흑마늘 농축액에 홍삼 미세분말을 3, 5, 7 및 10%씩 첨가하여 시료를 제조하였다. Based on the viscosity and flowability analysis results of the sample prepared according to Example 1 in order to set the optimum concentration of the micro ginseng powder as a condition setting condition for the production of concentrated beverages by mixing the black garlic concentrate and the micro ginseng powder, Was used for 60 brix, but the concentration was adjusted to 30 brix, which is the lowest concentration for beverage production using concentrate, considering the addition of other ingredients. As a result of preliminary evaluation based on sensory characteristics, it was appropriate to use less than 10% of red ginseng powder. Therefore, samples were prepared by adding 3, 5, 7 and 10% red ginseng powder to a certain amount of black garlic concentrate.
실험예 3 : 점도 측정 Experimental Example 3: Viscosity Measurement
각각의 시료는 실험예 1에서와 동일한 방법으로 점도를 측정하였으며, 각각의 시료에 대한 관능평가를 실시하였다. 이 때 점도는 상기와 동일하게 L3 스핀들을 사용하였으며, 점도계의 회전속도는 100 rpm으로 조정하였다. The viscosity of each sample was measured in the same manner as in Experimental Example 1, and the sensory evaluation was performed on each sample. At this time, an L3 spindle was used in the same manner as described above, and the rotation speed of the viscometer was adjusted to 100 rpm.
표 4에서 보는 바와 같이 홍삼분말의 사용량이 증가할수록, 흑마늘 농축액의 농도가 높을수록 점도는 더 높았다. 30 brix 흑마늘 농축액에 홍삼 분말을 혼합하였을 때 점도는 12.08~278.34 cPs의 범위였고, 60 brix 농축액과 혼합하였을 때는 73.42~840.46 cPs 였다. As shown in Table 4, as the amount of red ginseng powder was increased and the concentration of black garlic concentrate was higher, the viscosity was higher. The viscosity of 30 brix black ginger concentrate was in the range of 12.08 ~ 278.34 cPs when mixed with red ginseng powder and 73.42 ~ 840.46 cPs when mixed with 60 brix concentrate.
실험예Experimental Example 4 : 관능평가 4: Sensory evaluation
관능평가는 각각의 시료를 12 mL 씩 스틱형 무지 포장지에 담아 제공하였으며, 인삼 향, 흑마늘 향, 쓴맛, 단맛, 음용시 흐름성, 입안에서의 질감, 전체적인 기호도를 평가항목으로 하여 20~40대 남녀 20명을 대상으로 5점 측도법으로 실시하였다. The sensory evaluation was carried out by placing 12 mL of each sample in a stick type plain wrapping paper. The ginseng flavor, black garlic flavor, bitter taste, sweetness, flowability at the time of drinking, texture in the mouth, The subjects were 20 male and 5 female.
30과 60 brix의 흑마늘 농축액에 홍삼 미세분말을 3~10% 범위로 첨가한 시료들의 관능평가 결과는 도 1과 같다. 홍삼 분말을 10% 첨가한 시료의 경우 흑마늘 농축액의 농도와 상관없이 쓴맛과 인삼 향이 강하며, 입안에서의 질감에 대한 기호도가 낮아 전체적인 선호도도 낮았다. 또한 홍삼 분말 3% 첨가군의 경우도 홍삼과의 혼합제품임에도 불구하고, 흑마늘의 특성이 강하여 전체적인 선호도는 낮았다. 홍삼분말을 5%와 7% 첨가한 시료에서는 음용시 흐름성, 단맛, 입안에서의 질감에 대해 높은 선호도를 보였고, 인삼 향이나 흑마늘 향은 중간정도의 점수를 얻어 전체적인 기호도에서는 이들 시료의 선호도가 높았다. 전체적인 기호도를 중심으로 볼 때 홍삼분말을 5% 첨가하였을 때 가장 선호도가 높았는데, 30 brix에 홍삼 분말을 5% 첨가하였을 때에 비해 60 brix에 첨가하였을 때 더 선호도가 높았다. 이상의 결과로부터 흑마늘 농축액과 홍삼 미세분말 혼합액의 제조시 홍삼 미세분말의 적정 혼합농도는 5~7%이며, 보다 바람직하게는 5% 정도 첨가하는 것이 좋다.Fig. 1 shows the sensory evaluation results of the samples in which the red ginseng concentrate of 30 and 60 brix was added in the range of 3 to 10% of red ginseng fine powder. In the case of 10% red ginseng powder, bitterness and ginseng were strong regardless of concentration of black garlic concentrate, and overall preference was low due to low preference for texture in mouth. In addition, the group with 3% red ginseng powder was also a mixed product with red ginseng, but the overall preference was low due to the strong characteristics of black ginseng. In the samples containing 5% and 7% of red ginseng powder, the preference for flowability, sweetness and texture in the mouth was high, while the ginseng and black garlic flavor scores were medium, and the preference Respectively. The highest preference was obtained when 5% of red ginseng powder was added to 30 brix, compared with 5% of red ginseng powder added to 30 brix. From the above results, the optimum mixing concentration of the red ginseng micropowder during the production of the mixture of the black garlic concentrate and the red ginseng micropowder is 5 to 7%, more preferably about 5%.
이상의 실시예 1과 2의 결과로부터 흑마늘 농축액과 홍삼 분말을 혼합하여 고점도의 액상 농축음료를 제조함에 있어 적절한 흑마늘 농축액의 농도는 30~60 brix 이며, 이 때 홍삼 분말은 800 mesh 정도의 미세분말을 5~7% 혼합하는 것이 적절하다. 이 두 소재의 혼합만으로도 음용에 적합한 선호도를 가지는 고점도 액상 농축음료를 제조할 수 있으나 흑마늘이나 홍삼이 가지는 기능성에 더하여 기능성과 관능적 특성이 더 향상된 음료를 제조하기 위해서는 또 다른 소재를 첨가하는 것도 가능하다. From the results of Examples 1 and 2 above, it can be seen that the concentrated concentration of black garlic concentrate is 30 to 60 brix in preparing a concentrated liquid beverage having a high viscosity by mixing the black gum concentrate with the red ginseng powder, wherein the red ginseng powder has about 800 mesh fine powder It is appropriate to mix 5 to 7%. It is also possible to prepare a high viscosity liquid concentrated drink having a preference for drinking by mixing only the two materials. However, it is also possible to add another material in order to produce drinks having improved functionality and sensory characteristics in addition to the functions of black ginseng and red ginseng .
하기의 실시예는 기관지 염증을 개선하고, 기관지 건강에 도움을 줄 수 있는 음료를 제조하기 위한 것으로 본 발명의 의도를 명확하게 하기 위한 것이며, 본 발명의 범위를 한정하고자 하는 것은 아니다. The following examples are intended to clarify the intention of the present invention for improving the bronchial inflammation and for producing beverages which can contribute to bronchial health, and are not intended to limit the scope of the present invention.
실시예 3 : 흑마늘 농축액과 미세홍삼 분말 혼합물을 주재로 한 음료의 제조를 위한 혼합용 소재의 항염증 활성 검증 Example 3: Anti-inflammatory activity of mixed materials for the manufacture of beverages based on mixture of black garlic concentrate and micro ginseng powder
흑마늘 농축액과 홍삼 미세분말을 주재료로 하고, 기관지 건강에 도움이 되는 고농축 음료의 제조를 위하여 배 농축액 및 도라지 농축액을 혼합할 수 있다. 배는 변비예방, 숙취해소, 소화촉진, 항암효과, 고혈압예방, 기침, 가래, 기관지염, 해열작용, 이뇨작용이 있으며, 도라지는 면역력강화, 항암작용, 혈관질환예방, 호흡기질환완화, 항염증작용, 통증완화, 항산화작용, 해열작용, 항염증작용이 있는 것으로 알려져 있다. 배와 도라지는 전통적으로 민간에서 목 건강이나 환절기 감기 등의 예방 및 증상 완화를 위하여 섭취하여온 것으로 이들을 혼합하여 항염증 활성 및 면역증진 활성이 있는 고농축 음료를 제조할 수 있다. It is possible to mix the pear concentrate and the bellflower concentrate for the production of the highly concentrated beverage which is beneficial for bronchial health, using the black garlic concentrate and the red ginseng fine powder as the main ingredients. Boats are used for preventing constipation, relieving hangovers, promoting digestion, preventing cancer, preventing hypertension, coughing, phlegm, bronchitis, fever, diuretic action, platycodon enhancing immunity, anticancer activity, vascular disease prevention, respiratory disease alleviation, , Pain relief, antioxidant, antipyretic, and anti-inflammatory effects. Traditionally, pears and bellflower have been consumed for prevention and symptom relief such as neck health and seasonal cold in the private sector, and they can be mixed to produce highly concentrated beverages having anti-inflammatory activity and immunity enhancing activity.
잘게 세절한 시료 300 g과 증류수 3 L를 혼합하여 70℃에서 교반하면서 3시간 동안 추출한 뒤 거즈로 여과하여 동결 건조하여 보관용기에 보관하며 시료로 사용하였다. 분석을 위한 추출물 제조는 동결건조 시료 0.5 g을 취해 증류수를 가하여 25 mL가 되도록 한 후 30분간 진탕 추출한 후 여과지(No. 2, Advantec, Tokyo, Japan)로 여과한 여액을 농도 조절하여 총 폴리페놀 화합물 정량과 항산화 및 항염증 활성 측정용 시료액으로 사용하였다.300 g of finely divided sample and 3 L of distilled water were mixed and stirred at 70 ° C. for 3 hours. The mixture was filtered with gauze, freeze-dried, and stored in a storage container. To prepare the extract, 0.5 g of the lyophilized sample was added and distilled water was added to make 25 mL. After shaking for 30 minutes, the filtrate was filtered through a filter paper (No. 2, Advantec, Tokyo, Japan) The compound was used as a sample solution for quantitative determination of antioxidant and anti-inflammatory activity.
실험예 5 : 배와 도라지 추출물의 RAW 264.7 세포에 대한 독성평가 Experimental Example 5: Assessment of toxicity of RAW 264.7 cells on embryo and bellflower extracts
RAW 264.7 대식세포 증식에 대한 세포독성 평가를 위하여 마우스의 대식세포주인 RAW 264.7 세포는 한국세포주은행 (KCLB)에서 분양받았으며, 세포 배양을 위해 10% fetal bovine serum (FBS)와 1% penicillin-streptomycin을 포함하는 DMEM 배지를 사용하였다. 세포는 CO2 incubator (37℃, 5% CO2)에서 배양하였다. 추출물의 세포에 대한 독성 측정은 3-(4,5-dimethylthiazole-2-yl)-2,5??diphenyltetrazolium bromide (MTT) 환원방법을 이용하여 측정하였다. 세포를 96 well-plate에 well당 5×104개가 되도록 분주하고 24시간 부착시킨 후, 추출물들을 각기 일정한 농도로 희석하여 세포에 처리한 다음 30분 후 1 μg/mL lipopolysaccharide (LPS, Sigma Co, USA)를 처리하여 24시간 동안 배양하였다. 이후 시료를 포함하는 배지를 제거한 후 serum-free 배지와 5 mg/mL MTT 용액을 첨가하여 37℃에서 2시간 더 배양하여 DMSO를 분주하여 sonication하고 10분간 교반하여 용출시킨 뒤 570 nm에서 흡광도를 측정해 세포생존율을 구하였다. 세포생존율은 무처리군에 대한 백분율로 나타내었다.RAW 264.7 RAW 264.7 cells were purchased from the Korean Cell Line Bank (KCLB) for the evaluation of cytotoxicity against macrophage proliferation, and 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin Were used. Cells were cultured in CO 2 incubator (37 ° C, 5% CO 2 ). The toxicity of the extract to cells was measured by 3- (4,5-dimethylthiazole-2-yl) -2,5-diphenyltetrazolium bromide (MTT) reduction method. Cells were plated on 96-well plates at a density of 5 × 10 4 cells per well and incubated for 24 hours. Cells were treated with 1 μg / mL lipopolysaccharide (LPS, Sigma Co, USA) and cultured for 24 hours. After the medium containing the samples was removed, serum-free medium and 5 mg / mL MTT solution were added and incubated at 37 ° C for 2 hours. DMSO was sonicated and sonicated for 10 minutes. After elution, the absorbance was measured at 570 nm And cell viability was determined. Cell viability was expressed as a percentage of the untreated group.
도 2는 도라지와 배 추출물을 200, 400 및 600 μg/mL의 농도로 RAW 264.7 대식세포에 처리하였을 때 세포독성을 나타내는지를 확인한 결과이다. 실험된 시료별 모든 농도에서 세포는 97~109%의 생존율을 나타내어 세포독성은 없었다. 따라서 200~600 μg/mL의 농도 범위에서 이하의 실험을 실시하였다. FIG. 2 shows the results of confirming whether or not the bellflower and the vinegar extracts were cytotoxic when treated with RAW 264.7 macrophages at the concentrations of 200, 400 and 600 μg / mL. At all concentrations of the tested samples, the cells showed 97 ~ 109% survival rate and were not cytotoxic. Therefore, the following experiments were carried out in the concentration range of 200 to 600 μg / mL.
실험예 6 : 배와 도라지 추출물의 RAW 264.7 세포에 대한 NO 생성 억제 활성평가 Experimental Example 6: Evaluation of inhibitory activity of NO production on RAW 264.7 cells of pear and bellflower extracts
NO는 염증세포에 세균 유래 성분인 LPS 처리로 염증자극이 일어났을 때 염증반응을 유도하는 신호전달물질로 이후의 체내 방어를 위해 염증성 cytokine을 생성시킨다. 과다한 NO의 생성을 저해함으로서 염증반응이 과다하게 일어나는 것을 예방할 수 있다. NO is a signaling substance that induces an inflammatory response when inflammatory stimuli are caused by LPS treatment, which is a bacterial component of inflammatory cells, and produces inflammatory cytokines for subsequent body defense. By inhibiting excessive NO production, excessive inflammation reaction can be prevented.
LPS 처리 염증세포로부터 생성된 NO의 양을 세포 배양액 중에 존재하는 NO2 -의 형태로서 Griess 시약을 이용하여 측정하였다. 마우스 대식세포 RAW264.7를 well당 5×105 세포수로 24-well plate에 분주하여 24시간 배양한 후 각각의 추출물을 농도별로 처리한 다음, 30분 배양한 후에 LPS (1 μg/mL)를 처리하여 20시간 또는 24시간 배양한 후 세포 상등액을 회수하였다. 회수한 세포 배양 상등액을 원심분리 (1000 rpm, 10min, 4℃) 후, 50 μL를 취해 sulfanilamide solution 50 μL와 혼합하여 5분간 빛을 차단하고 반응시킨 다음 NED solution 50 μL와 혼합하여 상온에서 10분간 반응시켜, ELISA reader를 이용하여 540 nm에서 흡광도를 측정하였고, LPS 처리세포군에 대한 상대적인 생성율로 나타내었다. The amount of NO produced from LPS - treated inflammatory cells was measured using Griess reagent as the form of NO 2 - present in the cell culture medium. Mouse macrophages RAW264.7 were seeded in 24-well plates at a density of 5 × 10 5 cells per well, cultured for 24 hours, treated with LPS (1 μg / mL) for 30 minutes, And cultured for 20 hours or 24 hours. Then, the cell supernatant was recovered. 50 μL of the recovered cell culture supernatant was centrifuged (1000 rpm, 10 min, 4 ° C) and 50 μL of sulfanilamide solution was mixed with 50 μL of light for 5 minutes. Then, the resultant was mixed with 50 μL of NED solution and incubated at room temperature for 10 minutes The absorbance was measured at 540 nm using an ELISA reader and expressed as a relative production rate relative to the LPS-treated cells.
도 3에서와 같이 도라지와 배 추출물은 대식세포에서 NO 생성을 유의적으로 억제하여 LPS 처리에 따른 염증자극을 효과적으로 방어하는 활성이 있음을 알 수 있다. 이러한 효과는 배 추출물에 비해서는 도라지 추출물에서 더 높았다. As shown in FIG. 3, platelets and embryo extracts significantly inhibited NO production in macrophages, indicating that LPS treatment effectively inhibited inflammation. This effect was higher in the bellflower extract than in the pear extract.
실험예 7 : 배와 도라지 추출물의 RAW 264.7 세포에 대한 intracellular ROS 측정Experimental Example 7: Measurement of intracellular ROS in RAW 264.7 cells of embryo and bellflower extracts
홍마늘 음료 단일시료 및 그 혼합물 추출물의 intracellular ROS 측정은 intracellular ROS assay kit (Cell biolabs, USA)을 이용하여 측정하였다. 96 well black plate에 5×104 cell/well의 RAW 264.7 cell을 black plate에 분주한 후 배양하여 세포를 well에 부착시켜 serum free DMEM 배지로 교환하였다. 세포를 24시간 배양한 후 각 시료를 농도별로 처리하여 24시간, 37℃, 5% CO2 조건에서 배양하였다. PBS (pH 7.4)로 3회 세척한 후 1XDCFH-DA를 배지에 100 μL 첨가하여 1시간, 37℃, 5% CO2에서 배양한 후 또 다시 PBS로 3회 씻어냈다. Lysis buffer 100 μL를 첨가하여 혼합한 후 fluorescence microplate reader(perkinElmer, USA)를 이용하여 excitation 485 nm, emisssion 535 nm에서 형광을 측정하여 무처리군과 대조군에 대한 ROS 생성을 비교하여 상대적인 값으로 나타내었다. Intracellular ROS measurement of single samples of red garlic beverage and its mixture extracts was performed using intracellular ROS assay kit (Cell biolabs, USA). 5 × 10 4 cells / well of RAW 264.7 cells were plated on a black plate on a 96-well black plate, and the cells were cultured and adhered to wells and replaced with serum-free DMEM medium. Cells were cultured for 24 hours, treated with each concentration, and incubated for 24 hours at 37 ° C in 5% CO 2 Lt; / RTI > After washing three times with PBS (pH 7.4), 100 μL of 1XDCFH-DA was added to the medium, and incubated for 1 hour at 37 ° C and 5% CO2. Then, the cells were washed three times with PBS. After addition of 100 μL of lysis buffer, fluorescence was measured at excitation 485 nm and emisssion 535 nm using a fluorescence microplate reader (PerkinElmer, USA), and the ROS production for the untreated group and the control group was compared as a relative value .
ROS는 호흡과정 중 생성되는 물질로 각종 미생물을 사멸시키거나, 대식세포와 접촉시, NF-κB를 경유하여 면역과 염증성 사이토카인을 생산하는데 일조한다고 알려져 있다. 도 4에서와 같이 도라지와 배 추출물은 모두 대조군에 비해 유의적으로 ROS의 생성을 억제함으로써 항염증 활성을 나타내는데 기여할 것으로 기대된다. ROS is a substance that is produced during the respiration process and is known to kill various microorganisms or, in contact with macrophages, to produce immunity and inflammatory cytokines via NF-κB. As shown in FIG. 4, both of the bellflower and the extract of Bombyx mori were found to contribute to anti-inflammatory activity by inhibiting the production of ROS significantly compared to the control group.
실시예 4 : 흑마늘 농축액과 미세홍삼 분말 혼합물을 주재로 한 음료의 배합 비율 설정 Example 4: Setting the mixing ratio of beverages based on a mixture of black garlic concentrate and micro ginseng powder
상기의 실험예 5~7을 통해 항염증 활성이 확인된 배와 도라지를 이용하여 추출, 농축액을 제조하였다. 배 농축액은 박피하지 않은 배를 깨끗이 씻어 8등분 이상으로 절단한 후 부직포에 넣고 배의 무게 대비 10배의 물을 가하여 85~100℃에서 3~5시간 추출한 후 여액만을 모두 모아 60~90℃에서 가열하여 72 brix로 농축하여 제조하였다. 도라지 농축액은 흐르는 물에서 충분히 세척한 후 초음파 추출기에서 20분간 세척하여 조직 사이에 끼여 있는 흙이나 부산물을 제거한 다음 다시 흐르는 물에서 세척하였다. 이것으로 길이대로 5 cm 정도로 절단한 다음 부직포에 넣어서 도라지 무게 대비 20~25배의 물을 가하여 85~100℃에서 3~5시간 추출한 후 여액만을 모두 모아 70~95℃에서 가열하여 49 brix로 농축하여 제조하였다.The extracts and concentrates were prepared by using the pears and bellflower bottles whose anti-inflammatory activity was confirmed through the above Experimental Examples 5 to 7. The pear concentrate is washed with undiluted pears, cut into 8 equal portions or more, put into nonwoven fabric, and 10 times water is added to the weight of the pear, and extracted at 85 to 100 ° C for 3 to 5 hours. Only the filtrate is collected and dried at 60 to 90 ° C And then concentrated by heating to 72 brix. The bellflower concentrate was thoroughly washed with running water and then washed with an ultrasonic extractor for 20 minutes to remove soil or by-products between tissues and then washed with running water. This is cut into lengths of about 5 cm and then put into a nonwoven fabric. Twenty to twenty times as much water as the bellflower is added and extracted at 85 to 100 ° C for 3 to 5 hours. The filtrate is collected and heated at 70 to 95 ° C to be concentrated to 49 brix .
배와 도라지 농축액과 흑마늘 농축액, 홍삼 미세분말과 혼합한 음료 제조를 위한 배합비 설정을 위하여 아래와 같이 시료를 제조하여 이화학적 특성을 확인하였다. The physicochemical properties of the red germ extract and red germ extract, red ginseng concentrate and red ginseng powder were investigated.
실험예 8 : 혼합음료의 이화학적 특성 분석 Experimental Example 8: Analysis of physicochemical properties of mixed beverages
흑마늘, 도라지, 배 각각의 농축액을 제조한 후 홍삼 미세분말을 혼합한 고농축 음료 제조의 이화학적 특성 분석을 위하여 점도, 가용성 고형분 함량 및 총 폴리페놀 화합물의 함량을 분석하였다. 점도와 총 폴리페놀 화합물의 함량은 상기와 동일한 방법에 의해 분석하였으며, 가용성 고형분은 디지털 당도계를 이용하여 측정하였다. The viscosity, solids content and total polyphenolic compound content of the red ginseng, bellflower, and pear were analyzed for the physicochemical properties of the red bean ginseng powder. The viscosity and total polyphenol compound content were analyzed by the same method as above, and soluble solids were measured using a digital sugar meter.
(brix)Soluble solids
(brix)
점도는 홍삼 분말의 첨가량이 높은 B, D 및 F 시료에서 상대적으로 더 높았고, 이들 중에서도 흑마늘 농축액과 도라지 농축액의 첨가량이 모두 높은 F 시료의 점도가 가장 높았다. 가용성 고형분은 첨가되는 재료의 농도가 모두 높은 E 시료에서 36.47 brix로 가장 높았고, 여타 시료들은 30.60~33.70 brix의 범위였고, 총 페놀화합물의 함량도 4.45~4.95% mg/g의 범위로 시료간에 큰 차이가 없었다. Viscosity was higher in B, D and F samples with high red ginseng powder content. Among them, F samples showed the highest viscosity of both high concentration of black garlic concentrate and high concentration of gypsum concentrate. The soluble solids content was the highest at 36.47 brix in the E samples with the highest concentration of added material. The other samples were in the range of 30.60 to 33.70 brix, and the content of total phenolic compounds ranged from 4.45 to 4.95% mg / g. There was no difference.
실험예 9 : 혼합음료의 관능평가 Experimental Example 9: Sensory evaluation of mixed drinks
상기 실시예 4에 따라 제조한 음료 6종에 대한 관능평가를 25~45세의 남녀 20명을 대상으로 실시하였으며, 5점 측도법에 따라 해당 항목에 대한 선호도가 높거나 향이나 맛이 강할 경우 더 높은 점수를 부여하도록 하였다. The sensory evaluation of six beverages manufactured according to Example 4 was conducted on 20 men and women aged 25 to 45 years. When the preference for the item was high or the flavor or taste was strong according to the 5-point scale method So that a higher score can be given.
관능평가 결과(도 5) 인삼 향이나 입안에서의 질감은 시료간에 큰 차이가 없었으며, 음용시 흐름성은 점도가 가장 높았던 F 시료에서 가장 낮았고 여타 시료간에는 큰 차이가 없었다. 흑마늘 향이나 쓴맛이 시료에 따라 차이를 나타내었는데, 동일한 농도의 흑마늘 농축액이 사용되었더라도 첨가되는 부재료와 비율에 따라 관능적으로 느껴지는 흑마늘 향에는 차이가 있음을 알 수 있다. 이는 쓴맛도 동일한 경향으로 도라지 농축액과 홍삼 분말 특유의 맛으로 인해 이들의 첨가 비율이 높을수록 쓴맛은 더 높아지는 경향이었다. 전체적인 기호도는 A 시료에서 가장 높았고, 다음으로 C 시료에서 높았다. 이는 흑마늘, 홍삼 분말의 비율이 조화를 이루고 배 농축액의 첨가 비율이 높아 적절한 단맛을 제공하기 때문이다. The sensory evaluation results (Fig. 5) The ginseng flavor and texture in the mouth were not significantly different between the samples, and the flowability at the time of drinking was the lowest in the F samples having the highest viscosity, and there was no significant difference among the other samples. The black ginger flavor and bitter taste differed according to the sample. Even if the same concentration of black garlic concentrate was used, it can be understood that there is a difference in the flavor of the black ginger which is sensually felt depending on the added ingredient and ratio. The bitter taste tended to be the same with bitter taste, and the bitter taste tended to be higher with the addition ratio of the red ginseng powder and the red ginseng powder. The overall acceptability was highest in the A sample and then higher in the C sample. This is because the ratio of black ginseng and red ginseng powder is in harmony and the addition ratio of the concentrate is high, thereby providing an appropriate sweet taste.
이상의 결과로부터 흑마늘 농축액, 홍삼 분말, 배와 도라지 농축액을 함유하는 음료의 제조시 적절한 혼합비는 관능적으로 가장 선도로가 높은 A 배합비에 따라 제조된 것으로 설정할 수 있으며, A 재료의 이화학적 분석 결과를 토대로 최종 제품의 품질 기준 범위를 설정할 수 있다. From the above results, it can be determined that the proper blending ratio for producing beverages containing black garlic concentrate, red ginseng powder, pear and bellflower concentrate can be set to be prepared according to the A-blend ratio, which is the most sensible and the highest, and based on the result of physicochemical analysis The quality standard range of the final product can be set.
실시예 5 : 흑마늘 농축액과 미세홍삼 분말 혼합물을 주재로 한 음료의 제조 Example 5: Preparation of a beverage based on a mixture of black garlic concentrate and micro ginseng powder
흑마늘 농축액, 홍삼 분말, 배와 도라지 농축액을 함유하는 음료의 대량생산을 위하여 모든 재료를 일시에 혼합한 후 90℃에서 30분간 교반하면서 혼합하는 것이 관능적으로 우수한 혼합음료의 제조에 유리하다. It is advantageous to mix all the ingredients at once at 90 ° C. for 30 minutes for mass production of beverages containing black garlic concentrate, red ginseng powder, pear and bellflower concentrate, and stir for 30 minutes.
표 7은 재료의 혼합시 온도를 상온(25℃)과 고온(90℃)으로 달리하고, 온도에 따라 혼합방법을 달리하였을 때 이화학적 특성을 분석한 결과이다. 모든 시료에서 pH, 산도 및 가용성 고형분은 시료간에 차이가 없었다. 하지만 점도에서는 차이가 있었는데, 25℃와 90℃에서 각각 30분간 정치시킨 후 재료를 혼합한 경우와 재료를 혼합한 후 30분간 교반하면서 혼합한 경우를 비교하였을 때 온도에 따른 영향 보다는 교반의 실시여부가 더 영향을 미쳤다. 즉, 90℃에서 교반하면서 혼합하였을 때 관능평가 결과에서와 유사한 범위의 점도를 지니는 음료를 제조할 수 있다. 이 경우 25℃에서 교반하면서 혼합할 때에 비해 점도는 상대적으로 낮으며, 정치시키는 경우에 비해서는 더 높은 점성을 지니는 음료를 제조할 수 있다. 또한 90℃에서 30분 정도 교반하면서 혼합하는 과정을 거침으로써 혼합된 재료들이 서로 충분히 혼합되며, 살균되는 효과를 동시에 얻을 수 있다. Table 7 shows the results of analyzing the physicochemical properties of the materials when they were mixed at room temperature (25 ° C) and high temperature (90 ° C) and the mixing method was changed according to temperature. PH, acidity and soluble solids in all samples were not different between the samples. However, there was a difference in viscosity. When the mixture was allowed to stand at 25 ° C and 90 ° C for 30 minutes, and the materials were mixed and the materials were mixed and stirred for 30 minutes, Had more influence. That is, a beverage having a viscosity in a range similar to that of the sensory evaluation result can be produced when mixed at 90 DEG C with stirring. In this case, the viscosity is relatively low as compared with mixing with stirring at 25 DEG C, and a beverage having a higher viscosity than when it is allowed to stand can be produced. Also, by mixing the mixture at 90 DEG C for about 30 minutes, the mixed materials are sufficiently mixed with each other, and the effect of sterilizing can be obtained at the same time.
실시예 6 : 본 발명의 교반기에 따른 흑마늘 농축액과 미세홍삼 분말 혼합음료의 제조 Example 6: Preparation of mixed black bean gum concentrate and micro ginseng powder according to the stirrer of the present invention
도 6(가),(나) 및 (다)는 본 발명의 바람직한 일실시예에 따른 교반장치의 작동 상태도를 나타낸 것이다. 본 발명에 따른 교반기(100)는 혼합물을 교반하는 교반조(10)와 교반조(10) 내부로 위치되며 하단에 배출공(22)이 형성된 실린더(20), 상기 실린더(20) 내벽에 밀착되는 말착부(32)를 구비한 피스톤(30), 모터(42)의 회전력으로 회전되는 회전축(44)의 끝단에 부착되어 교반작동하는 교반날개(40)를 포함하여 구성된다. 6 (a), 6 (b) and 6 (c) show operation states of an agitation apparatus according to a preferred embodiment of the present invention. The
상기 교반기(100)에 혼합하고자 하는 혼합물의 분말은 교반조(10)에 넣고, 액상은 실린더(20)에 넣어서 교반날개(40)를 작동시켜서 혼합한다. 피스톤(30)의 작동에 의해 실린더(20)에 저장된 액이 배출공(22)으로 흘러내리면서 교반날개(40)의 작동으로 혼합시키고자 하는 분말이나 액이 잘 혼합되게 된다. 1차 교반만으로 혼합물이 잘 혼합될 수도 있지만 혼합물간의 균질화를 위해 2차 교반을 시키는 것이 바람직하다. The powder of the mixture to be mixed in the
2차 교반을 위해 다른 용기로 혼합물을 옮기지 않고 도 6(다)와 같이, 그대로 피스톤을 당기면 교반조에 교반된 혼합물이 실린더 내로 전부 흡입된다. 혼합물을 전부 흡입한 후에 다시 교반날개를 작동시키고, 피스톤을 눌러서 혼합물이 배출공을 통해 교반조로 배출되면서 교반날개에 의해 다시 교반된다. 이와 같은 교반기를 사용하게 되면 혼합시키고자 하는 분말이나 액이 충분히 균질화 된다.When the piston is pulled as it is, as shown in Fig. 6 (C) without transferring the mixture to another container for secondary agitation, the agitated mixture is completely sucked into the cylinder. After the mixture is completely inhaled, the stirring vane is operated again, and the mixture is stirred again by the stirring wing while the piston is pressed and the mixture is discharged through the discharge hole into the stirring tank. When such an agitator is used, the powder or liquid to be mixed is sufficiently homogenized.
이상의 설명은 본 발명의 기술 사상을 예시적으로 설명한 것에 불과한 것으로서, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자라면 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 다양한 수정 및 변형이 가능할 것이다. 따라서 본 발명에 개시된 실시예들은 본 발명의 기술 사상을 한정하기 위한 것이 아니라 설명하기 위한 것이고, 이러한 실시예에 의하여 본 발명의 기술 사상의 범위가 한정되는 것은 아니다. 본 발명의 보호 범위는 아래 청구범위에 의하여 해석되어야 하며, 그와 동등한 범위 내에 있는 모든 기술 사상은 본 발명의 권리범위에 포함되는 것으로 해석되어야 할 것이다.The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are not intended to limit the scope of the present invention but to limit the scope of the technical idea of the present invention. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.
Claims (4)
A high-viscosity beverage composition comprising a black garlic concentrate and red ginseng powder, characterized in that 800 g of micro ginseng powder is mixed with 5 ~ 7% of the weight of the germanium concentrate to the black garlic concentrate concentrated at 30 ~ 60 brix after extracting the black garlic.
30 to 60 brix of black garlic concentrate 20 to 40 wt.%, Micro ginseng powder 3 to 8 wt.%, 68 to 75 brix concentrate 9 to 19 wt.% And 45 to 53 brix bellflower concentrate 0.5 to 1.5 wt.%, Purified water 35 to 65 wt. % ≪ / RTI > concentrated red ginseng powder and red ginseng powder.
A method for producing a highly viscous beverage comprising a black garlic concentrate and red ginseng powder, which comprises mixing 30 to 60 brix of black garlic concentrate, micro ginseng powder, 68 to 75 brix concentrate, and bellflower concentrate in a stirrer while stirring at 90 ° C for 30 minutes
상기 교반기는 교반조와 상기 교반조 내부로 위치되며 하단에 배출공이 형성된 실린더; 상기 실린더 내벽에 밀착되는 말착부를 구비한 피스톤; 모터의 회전력으로 회전되는 회전축의 끝단에 부착되어 교반작동하는 교반날개를 포함하여 구성된 교반장치로 이루어진 것을 특징으로 한 흑마늘 농축액과 홍삼분말을 포함하는 고점도 음료의 제조방법.The method of claim 3,
Wherein the agitator comprises a stirrer, a cylinder positioned inside the agitator, and a discharge hole formed at a lower end thereof; A piston having an anchor portion which is in close contact with the inner wall of the cylinder; And a stirrer attached to an end of a rotating shaft rotated by a rotating force of the motor and stirring the mixed powder. The method of producing a highly viscous beverage comprising a black garlic concentrate and red ginseng powder.
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KR20210024726A (en) * | 2019-08-26 | 2021-03-08 | (재)남해마늘연구소 | Black garlic extract, black garlic drink comprising it and composition for improving male health comprising it |
KR20210091614A (en) * | 2020-01-14 | 2021-07-22 | (재)남해마늘연구소 | Beverage composition comprising active ingredients of black garlic and red ginseng, and manufacturing method thereof |
KR20220095077A (en) * | 2020-12-29 | 2022-07-06 | 재단법인 금산인삼약초산업진흥원 | A composite for health functional food hanving excellent anti-inflammatory and anti-oxidant, and health functional food |
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KR20210024726A (en) * | 2019-08-26 | 2021-03-08 | (재)남해마늘연구소 | Black garlic extract, black garlic drink comprising it and composition for improving male health comprising it |
KR20210091614A (en) * | 2020-01-14 | 2021-07-22 | (재)남해마늘연구소 | Beverage composition comprising active ingredients of black garlic and red ginseng, and manufacturing method thereof |
KR20220095077A (en) * | 2020-12-29 | 2022-07-06 | 재단법인 금산인삼약초산업진흥원 | A composite for health functional food hanving excellent anti-inflammatory and anti-oxidant, and health functional food |
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